pycom06g19280

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008073
Physical Location & Seq
Reverse (-)
22737651 .. 22739211
1561 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19280.1

Sequence Viewer

Length: 1215 bp
ATGCAAACTCATTTTTCGGAGAAGAAAATGCCATGTTCAGCTGAGGAAGCACTAGTTAGGCTCTTCTACAACACCTCCTCCGCCTTCCACCTCCTCCTCCTCTTCCTCTACCTCTCCTCCATTTTTCTTTTCAAACTCTTCTACTTTGTTGGTAGCAACCCTATTTTCTCAAGAAGCCAGAATGGATACGAAGACTACGTGTTTTCGGACGAAGAAGAAGAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGACGAACAAAGGCAGTTTCATGTTCCTGGTCACCAATACTGCAGTACCATAAATGATCATGATCTGGTGGCTGACATCATTCACGGCGGCGAATCACTGATGTTTTTGCCTAACAATAACCTTCAGCATCATCATCAAAAAAGTTTTTCGGAAGACCAATTCATTAGCGCTCGGGAGAGCTTAATAATTGAAGACCGCCCCGATGAATCTTTGCATGAGTCACCACAAGGTTTAGAATCTGAACATGACGATCAAGGTGCGGAGGAAATCCCCGTGAAAGATACAGTTTCAGTTCTCAATTCTGTTGCGAAGGATCGTGTGTGGCCCACATCTCCGATAACTATAGAGCTACATAAAAGTGACAAAAATGGTGACGGTAATTGTGATGAACATAATACTGAAATCAATAGGGCAGATCAGACGCATTTATCCAGTGACGAAAATTTTGTGGTTTTCGGGGCATCACAATTGAAGAACAAGAAGTTCCAAGTTCAAGACAATAAAGAGGATGAAATTTTTGGCGACTCAACTGTTGGATCAACTTCTAAGAGCTCTTCTGAATGGAGAAGCTCAATTAATTGCAGGGATTCTGTCACTGAAGATCCCTTTTCTTCCTCATCCCGGCGAAGCTGCCCCAAATGGGAGTCTTACACAGTCTTCCAAAAGTATGATGAAGAAATGATGTTCCTAGACAGAATTAGTGCCCAAAAGCTCCATGAAACAGAATCACTTAAGTCTATTCAAGTTTGTCCAAGATCAATTTCGGAAAGGATTGTTCATAGAATCTCTACGGCAACAAAAAGGCTGCCTGATGTACGTCACAACCCTTATCATGAGCTGGAGGCTGCTTATGTTGCACAAATTTGCTTAACATGGGAAGCACTTAATTGGAACTACAAGAATTTTGAACAAAAAAGGGCATCACGAAGTGAGTTTGATCCATCGGCGTTCCTGGTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

405

Amino Acids

46.74

Weight (kDa)

4.79

Isoelectric Point (pI)

62.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 362 - 394 2e-10 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 81, 339, 448, 512
AclWI GGATC 4 cut(s) 573, 796, 848, 1184
AcsI RAATTY 4 cut(s) 694, 765, 1113, 1153
AcuI CTGAAG 2 cut(s) 359, 870
AdeI CACNNNGTG 1 cut(s) 1181
AfaI GTAC 2 cut(s) 298, 1068
AfeI AGCGCT 1 cut(s) 421
AfiI CCNNNNNNNGG 2 cut(s) 873, 892
AflII CTTAAG 1 cut(s) 983
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 6 cut(s) 133, 443, 724, 746, 995, 1160
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 2 cut(s) 277, 1203
AjuI GAANNNNNNNTTGG 2 cut(s) 997, 1029
AluBI AGCT 8 cut(s) 41, 432, 601, 804, 822, 882, 964, 1090
AluI AGCT 8 cut(s) 41, 432, 601, 804, 822, 882, 964, 1090
Alw21I GWGCWC 1 cut(s) 806
AlwI GGATC 4 cut(s) 573, 796, 848, 1184
Ama87I CYCGRG 1 cut(s) 423
Aor51HI AGCGCT 1 cut(s) 421
AoxI GGCC 1 cut(s) 575
ApeKI GCWGC 3 cut(s) 882, 1057, 1097
ApoI RAATTY 4 cut(s) 694, 765, 1113, 1153
ArsI GACNNNNNNTTYG 2 cut(s) 680, 712
AseI ATTAAT 1 cut(s) 828
AspLEI GCGC 1 cut(s) 422
AspS9I GGNCC 1 cut(s) 576
AsuC2I CCSGG 1 cut(s) 874
AsuHPI GGTGA 3 cut(s) 275, 465, 635
AvaI CYCGRG 1 cut(s) 423
BaeGI GKGCMC 1 cut(s) 958
BaeI ACNNNNGTAYC 2 cut(s) 280, 313
BanII GRGCYC 1 cut(s) 806
BbsI GAAGAC 5 cut(s) 198, 258, 411, 450, 901
Bbv12I GWGCWC 1 cut(s) 806
BbvCI CCTCAGC 1 cut(s) 42
BbvI GCAGC 3 cut(s) 869, 1044, 1084
BccI CCATC 1 cut(s) 1201
BceAI ACGGC 2 cut(s) 352, 1059
BcgI CGANNNNNNTGC 2 cut(s) 491, 525
BciT130I CCWGG 2 cut(s) 279, 1205
BciVI GTATCC 1 cut(s) 179
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 874
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 2 cut(s) 53, 941
BfmI CTRYAG 2 cut(s) 292, 594
BfoI RGCGCY 1 cut(s) 423
BfrI CTTAAG 1 cut(s) 983
BfuI GTATCC 1 cut(s) 179
BisI GCNGC 4 cut(s) 340, 883, 1058, 1098
BlsI GCNGC 4 cut(s) 341, 884, 1059, 1099
Bme1390I CCNGG 3 cut(s) 279, 874, 1205
BmeT110I CYCGRG 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 576
BmrFI CCNGG 3 cut(s) 279, 874, 1205
BmsI GCATC 3 cut(s) 388, 722, 1181
BpiI GAAGAC 5 cut(s) 198, 258, 411, 450, 901
BpmI CTGGAG 1 cut(s) 1112
Bpu10I CCTNAGC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 874
BsaAI YACGTR 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 312
BsaXI ACNNNNNCTCC 6 cut(s) 59, 62, 89, 92, 101, 131
Bsc4I CCNNNNNNNGG 2 cut(s) 873, 892
Bse1I ACTGG 1 cut(s) 684
Bse8I GATNNNNATC 1 cut(s) 312
BseBI CCWGG 2 cut(s) 279, 1205
BseGI GGATG 2 cut(s) 766, 869
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 2 cut(s) 873, 892
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 684
BseSI GKGCMC 1 cut(s) 958
BseXI GCAGC 3 cut(s) 869, 1044, 1084
BshFI GGCC 1 cut(s) 577
BsiHKAI GWGCWC 1 cut(s) 806
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 1 cut(s) 874
BslI CCNNNNNNNGG 2 cut(s) 873, 892
BsnI GGCC 1 cut(s) 577
BsoBI CYCGRG 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 806, 958
Bsp143I GATC 9 cut(s) 307, 313, 502, 565, 667, 788, 853, 1007, 1189
BspACI CCGC 4 cut(s) 81, 339, 448, 512
BspANI GGCC 1 cut(s) 577
BspCNI CTCAG 1 cut(s) 34
BspHI TCATGA 2 cut(s) 310, 1084
BspMAI CTGCAG 1 cut(s) 296
BspPI GGATC 4 cut(s) 573, 796, 848, 1184
BspQI GCTCTTC 2 cut(s) 68, 811
BspTI CTTAAG 1 cut(s) 983
BsrI ACTGG 1 cut(s) 684
BssMI GATC 9 cut(s) 307, 313, 502, 565, 667, 788, 853, 1007, 1189
Bst2UI CCWGG 2 cut(s) 279, 1205
Bst4CI ACNGT 4 cut(s) 538, 629, 784, 907
Bst6I CTCTTC 5 cut(s) 68, 107, 143, 213, 811
BstAFI CTTAAG 1 cut(s) 983
BstBAI YACGTR 1 cut(s) 199
BstDEI CTNAG 2 cut(s) 42, 798
BstEII GGTNACC 1 cut(s) 281
BstF5I GGATG 2 cut(s) 766, 869
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 1 cut(s) 422
BstKTI GATC 9 cut(s) 310, 316, 505, 568, 670, 791, 856, 1010, 1192
BstMBI GATC 9 cut(s) 307, 313, 502, 565, 667, 788, 853, 1007, 1189
BstMWI GCNNNNNNNGC 2 cut(s) 47, 1106
BstNI CCWGG 2 cut(s) 279, 1205
BstPI GGTNACC 1 cut(s) 281
BstSCI CCNGG 3 cut(s) 277, 872, 1203
BstSFI CTRYAG 2 cut(s) 292, 594
BstSLI GKGCMC 1 cut(s) 958
BstV1I GCAGC 3 cut(s) 869, 1044, 1084
BstV2I GAAGAC 5 cut(s) 198, 258, 411, 450, 901
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 1 cut(s) 577
BtsCI GGATG 2 cut(s) 766, 869
BtsIMutI CAGTG 3 cut(s) 347, 691, 846
CciI TCATGA 2 cut(s) 310, 1084
CfoI GCGC 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 576
CseI GACGC 1 cut(s) 682
Csp6I GTAC 2 cut(s) 297, 1067
CviAII CATG 8 cut(s) 33, 272, 311, 467, 497, 968, 1085, 1125
CviQI GTAC 2 cut(s) 297, 1067
DdeI CTNAG 2 cut(s) 42, 798
DpnI GATC 9 cut(s) 309, 315, 504, 567, 669, 790, 855, 1009, 1191
DpnII GATC 9 cut(s) 307, 313, 502, 565, 667, 788, 853, 1007, 1189
DraIII CACNNNGTG 1 cut(s) 1181
Eam1104I CTCTTC 5 cut(s) 68, 107, 143, 213, 811
EarI CTCTTC 5 cut(s) 68, 107, 143, 213, 811
EciI GGCGGA 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 804
Eco24I GRGCYC 1 cut(s) 806
Eco47III AGCGCT 1 cut(s) 421
Eco53kI GAGCTC 1 cut(s) 804
Eco57I CTGAAG 2 cut(s) 359, 870
Eco88I CYCGRG 1 cut(s) 423
Eco91I GGTNACC 1 cut(s) 281
EcoICRI GAGCTC 1 cut(s) 804
EcoO65I GGTNACC 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 277, 1203
EcoT38I GRGCYC 1 cut(s) 806
FaeI CATG 8 cut(s) 36, 275, 314, 470, 500, 971, 1088, 1128
FatI CATG 8 cut(s) 32, 271, 310, 466, 496, 967, 1084, 1124
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 4 cut(s) 340, 883, 1058, 1098
FokI GGATG 2 cut(s) 773, 856
FriOI GRGCYC 1 cut(s) 806
Fsp4HI GCNGC 4 cut(s) 340, 883, 1058, 1098
FspBI CTAG 2 cut(s) 53, 941
GlaI GCGC 1 cut(s) 421
GluI GCNGC 4 cut(s) 340, 883, 1058, 1098
GsuI CTGGAG 1 cut(s) 1112
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 1 cut(s) 577
HapII CCGG 1 cut(s) 874
HgaI GACGC 1 cut(s) 682
HhaI GCGC 1 cut(s) 422
Hin1II CATG 8 cut(s) 36, 275, 314, 470, 500, 971, 1088, 1128
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HinfI GANTC 9 cut(s) 344, 458, 470, 488, 776, 839, 896, 977, 1035
HpaII CCGG 1 cut(s) 874
HphI GGTGA 3 cut(s) 275, 465, 635
Hpy188I TCNGA 8 cut(s) 19, 208, 403, 493, 588, 672, 811, 1018
Hpy188III TCNNGA 6 cut(s) 171, 311, 425, 746, 1085, 1176
HpyAV CCTTC 3 cut(s) 94, 383, 556
HpyCH4III ACNGT 4 cut(s) 538, 629, 784, 907
HpyCH4IV ACGT 2 cut(s) 198, 1069
HpyCH4V TGCA 5 cut(s) 4, 294, 466, 834, 1109
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 1106
HpyF3I CTNAG 2 cut(s) 42, 798
HpySE526I ACGT 2 cut(s) 198, 1069
Hsp92II CATG 8 cut(s) 36, 275, 314, 470, 500, 971, 1088, 1128
HspAI GCGC 1 cut(s) 420
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 9 cut(s) 307, 313, 502, 565, 667, 788, 853, 1007, 1189
LguI GCTCTTC 2 cut(s) 68, 811
LmnI GCTCC 1 cut(s) 969
Lsp1109I GCAGC 3 cut(s) 869, 1044, 1084
LweI GCATC 3 cut(s) 388, 722, 1181
MaeI CTAG 2 cut(s) 53, 941
MaeII ACGT 2 cut(s) 198, 1069
MaeIII GTNAC 7 cut(s) 281, 471, 611, 623, 686, 844, 1070
MalI GATC 9 cut(s) 309, 315, 504, 567, 669, 790, 855, 1009, 1191
MboI GATC 9 cut(s) 307, 313, 502, 565, 667, 788, 853, 1007, 1189
MfeI CAATTG 1 cut(s) 719
MflI RGATCY 1 cut(s) 853
MhlI GDGCHC 2 cut(s) 806, 958
MlyI GAGTC 3 cut(s) 479, 770, 905
MmeI TCCRAC 1 cut(s) 766
MseI TTAA 5 cut(s) 434, 828, 984, 1121, 1137
MslI CAYNNNNRTG 1 cut(s) 609
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 1 cut(s) 983
MspI CCGG 1 cut(s) 874
MspR9I CCNGG 3 cut(s) 279, 874, 1205
MunI CAATTG 1 cut(s) 719
MvaI CCWGG 2 cut(s) 279, 1205
MwoI GCNNNNNNNGC 2 cut(s) 47, 1106
NciI CCSGG 1 cut(s) 874
NdeII GATC 9 cut(s) 307, 313, 502, 565, 667, 788, 853, 1007, 1189
NlaIII CATG 8 cut(s) 36, 275, 314, 470, 500, 971, 1088, 1128
NmuCI GTSAC 7 cut(s) 281, 471, 611, 623, 686, 844, 1070
PagI TCATGA 2 cut(s) 310, 1084
PciSI GCTCTTC 2 cut(s) 68, 811
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 6 cut(s) 344, 458, 488, 839, 977, 1035
PkrI GCNGC 4 cut(s) 341, 884, 1059, 1099
PleI GAGTC 3 cut(s) 478, 770, 904
PpsI GAGTC 3 cut(s) 478, 770, 904
Ppu21I YACGTR 1 cut(s) 199
PshBI ATTAAT 1 cut(s) 828
Psp124BI GAGCTC 1 cut(s) 806
Psp6I CCWGG 2 cut(s) 277, 1203
PspEI GGTNACC 1 cut(s) 281
PspGI CCWGG 2 cut(s) 277, 1203
PspPI GGNCC 1 cut(s) 576
PstI CTGCAG 1 cut(s) 296
PsuI RGATCY 1 cut(s) 853
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 2 cut(s) 298, 1068
RsaNI GTAC 2 cut(s) 297, 1067
RseI CAYNNNNRTG 1 cut(s) 609
SacI GAGCTC 1 cut(s) 806
SapI GCTCTTC 2 cut(s) 68, 811
SaqAI TTAA 5 cut(s) 434, 828, 984, 1121, 1137
SatI GCNGC 4 cut(s) 340, 883, 1058, 1098
Sau3AI GATC 9 cut(s) 307, 313, 502, 565, 667, 788, 853, 1007, 1189
Sau96I GGNCC 1 cut(s) 576
SchI GAGTC 3 cut(s) 479, 770, 905
ScrFI CCNGG 3 cut(s) 279, 874, 1205
SduI GDGCHC 2 cut(s) 806, 958
SfaNI GCATC 3 cut(s) 388, 722, 1181
SfcI CTRYAG 2 cut(s) 292, 594
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 2 cut(s) 169, 983
SmoI CTYRAG 2 cut(s) 169, 983
SpeI ACTAGT 1 cut(s) 52
SsiI CCGC 4 cut(s) 81, 339, 448, 512
SspMI CTAG 2 cut(s) 53, 941
SstI GAGCTC 1 cut(s) 806
StyD4I CCNGG 3 cut(s) 277, 872, 1203
TaaI ACNGT 4 cut(s) 538, 629, 784, 907
TaiI ACGT 2 cut(s) 201, 1072
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 6 cut(s) 344, 458, 488, 839, 977, 1035
Tru1I TTAA 5 cut(s) 434, 828, 984, 1121, 1137
Tru9I TTAA 5 cut(s) 434, 828, 984, 1121, 1137
TscAI CASTG 3 cut(s) 354, 691, 853
TseFI GTSAC 7 cut(s) 281, 471, 611, 623, 686, 844, 1070
TseI GCWGC 3 cut(s) 882, 1057, 1097
Tsp45I GTSAC 7 cut(s) 281, 471, 611, 623, 686, 844, 1070
TspDTI ATGAA 8 cut(s) 260, 403, 471, 654, 777, 939, 984, 1019
TspRI CASTG 3 cut(s) 354, 691, 853
Vha464I CTTAAG 1 cut(s) 983
VspI ATTAAT 1 cut(s) 828
XapI RAATTY 4 cut(s) 694, 765, 1113, 1153
XspI CTAG 2 cut(s) 53, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.