MD14G1226800.v1.1

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008073
Physical Location & Seq
Reverse (-)
30808947 .. 30812257
3311 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1226800.v1.1.491

Sequence Viewer

Length: 1821 bp
ATGCCATGTTCAGCTGAGGAAGCACTTGTTAGGCTCTTCAACAACACTTCCTCCTCCTTCCTCCTCCTCCTCCTCTTCCTCTACGTCTCCTCCATTTTTCTTTTCAAACTCTTCTACTTTGTCGGCAGCAACCCTATTTTCCCAAGAAGCCAGAATGGTTGCGAAGACTACGTGTTTTCGGATGAAGAAGAGGAGGAGGAGGAAGAAGAAGAATTAGAAGAAGACGAAGAAGAAGAAGAAGACGAAGAAGAAGACGAAGAAAGGCAGTTTCATGTTCATAGTCACCAATACTGCAGAACCATAAATGATCATGATCTGGTGGCTGACATCATACGCGGCAACGAATCATTAAGGTTTTTGCCCAACCATAACCTTCAGCATCATCATCAAAAAAAAAATTCCGAAGACCAATTCATTAGCGCACGGGAGAGCTTAATAATTGAAGAACGCCAAGATGAATCTTTGCATGAGTCACCACAAGGCTCAGAATCTGAATATGAAGATCAAGGCGTGGAGGAAATCCCAGTGAAAGAAACAGTTTCAGTTCTCAATTCTGTCGCGAATGATCGAGTGTGGTCCACATCTCCGATAACTATAGAACTAGATAAAAGTGACAAAAATGGTGACGATAATTGTGATGAACACAATACTGAAATCAATAGGGCAGATCAGACGCATTTATCCGGTGACGAAAGTTTTGTTGTTTACGGGCCATCACAATTGAAGAACAAGAAGTTCCAAGTTCAAGACAATAAAGAGGATGAAATTTTTGGTGACTCAACTGTTGGATCAACTTCTAAGAGCTCTTCTGAATGGAGAAGCTCAATTAACTGCAGGGATTCTGCCACTGAAGATCCTTTTTCTTCCTCGTCCCGGAGAAGCTGCCCCAAATGGGAGTCTTACACAGTCTTCCAAAAGTATGATGAGGAAATGATGTTCTTAGACAGAATTAGTGCCCAAAAGCTCCATGAAACAGAATCACTTAAGTCTATTCAAGTTTGTCCAAGATCAATTTCGGAAAGGATAGTTCATAGAATCTCTATGGCAACAAAAAGGCCGCCTGATGTACGTCACAACCCTTATCATGAGCTGGAGGCTGCTTACGTTGCACAAATTTGCTTAACATGGGAAGCACTTAATTGGAACTACAAGAATTTTGAGCAAAAAAGGGCATCACGACGTGAAATTGATCCCGGCGTTCCTGGCGAATTAGCACAGCAATTTCAGCAATTTCAAGTGCTTCTACGAAGATATGTAGAGAATGAGCCTTATGAGCAAGGCAGGAGGCCAGAGATTTATGCTAGGATGAGGCTTTTGGCACCAAAGTTACTTCAAGTGCCAGAATATAGAGTTTCAGAAGATGGTGAGCATGAAAAGGACGAAGGTTTTGGCTCAAAAATTTCATCAGCTGCATTTTTAATGATAATGGAAGACGGAATCCGAACGTTCATGGATTTTCTCAAGGCTGATAAGGAAAAACCCTGCCAGAAATTAGTATCCATGTTCAAAAGAAAACGAAAAGGTTCAGTTGATCCCACTCTTCTCCATCTGATGAAGAAAGTCAATCAAAAAAAGAAGATGAAAATCAAAGATCTTCGACGTTCCCACAAATGCCTGAGAAAGCGAAGCTTGAAGGTGGAGGAAGAGATGGAGATACTGATGGGTCTAATTGACCTAAAATTGGTGTCTAGGGTTTTGAGAATGACCGACCTAAATGCAGAACAATTACATTGGTGCGAAGCAAAGATGAGCAAAGTGAGAGTTCTGGATGGGAAAAGATACCAAAGAGATTCGTCCCCACTTTTCTTCCCAGCACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

607

Amino Acids

70.86

Weight (kDa)

5.21

Isoelectric Point (pI)

64.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 362 - 604 1.1e-88 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1318
AccII CGCG 2 cut(s) 336, 560
AciI CCGC 2 cut(s) 336, 1058
AclI AACGTT 1 cut(s) 1445
AclWI GGATC 4 cut(s) 796, 848, 1184, 1526
AcsI RAATTY 5 cut(s) 397, 765, 1113, 1153, 1398
AcuI CTGAAG 2 cut(s) 359, 870
AdeI CACNNNGTG 1 cut(s) 1181
AfaI GTAC 1 cut(s) 1068
AfiI CCNNNNNNNGG 3 cut(s) 873, 892, 1681
AflII CTTAAG 1 cut(s) 983
AflIII ACRYGT 1 cut(s) 171
AjiI CACGTC 1 cut(s) 1181
AjnI CCWGG 1 cut(s) 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 997, 1029
AloI GAACNNNNNNTCC 2 cut(s) 920, 952
AluBI AGCT 9 cut(s) 14, 432, 804, 822, 882, 964, 1090, 1409, 1629
AluI AGCT 9 cut(s) 14, 432, 804, 822, 882, 964, 1090, 1409, 1629
Alw21I GWGCWC 1 cut(s) 806
Alw26I GTCTC 1 cut(s) 91
AlwI GGATC 4 cut(s) 796, 848, 1184, 1526
AlwNI CAGNNNCTG 1 cut(s) 491
AoxI GGCC 3 cut(s) 710, 1055, 1286
ApeKI GCWGC 4 cut(s) 126, 882, 1097, 1409
ApoI RAATTY 5 cut(s) 397, 765, 1113, 1153, 1398
ArsI GACNNNNNNTTYG 4 cut(s) 680, 712, 1370, 1402
Asp700I GAANNNNTTC 1 cut(s) 1522
AspLEI GCGC 1 cut(s) 422
AspS9I GGNCC 2 cut(s) 576, 710
AsuC2I CCSGG 2 cut(s) 874, 1194
AsuHPI GGTGA 6 cut(s) 275, 465, 635, 698, 785, 1376
AvaII GGWCC 1 cut(s) 576
BaeGI GKGCMC 1 cut(s) 958
BanI GGYRCC 1 cut(s) 1318
BanII GRGCYC 1 cut(s) 806
BbsI GAAGAC 7 cut(s) 171, 228, 246, 258, 411, 901, 1437
Bbv12I GWGCWC 1 cut(s) 806
BbvCI CCTCAGC 1 cut(s) 15
BbvI GCAGC 4 cut(s) 138, 869, 1084, 1396
BccI CCATC 6 cut(s) 721, 1355, 1554, 1642, 1654, 1763
BcgI CGANNNNNNTGC 2 cut(s) 1697, 1731
BciT130I CCWGG 1 cut(s) 1203
BciVI GTATCC 1 cut(s) 1507
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 2 cut(s) 874, 1194
BcoDI GTCTC 1 cut(s) 91
BfaI CTAG 3 cut(s) 602, 1302, 1689
BfmI CTRYAG 3 cut(s) 292, 594, 832
BfrI CTTAAG 1 cut(s) 983
BfuI GTATCC 1 cut(s) 1507
BglII AGATCT 1 cut(s) 1591
BisI GCNGC 6 cut(s) 127, 337, 883, 1058, 1098, 1410
BlsI GCNGC 6 cut(s) 128, 338, 884, 1059, 1099, 1411
Bme1390I CCNGG 3 cut(s) 874, 1194, 1203
Bme18I GGWCC 1 cut(s) 576
BmgBI CACGTC 1 cut(s) 1181
BmgT120I GGNCC 2 cut(s) 576, 710
BmiI GGNNCC 1 cut(s) 1320
BmrFI CCNGG 3 cut(s) 874, 1194, 1203
BmrI ACTGGG 1 cut(s) 518
BmsI GCATC 2 cut(s) 388, 1181
BmuI ACTGGG 1 cut(s) 518
BpiI GAAGAC 7 cut(s) 171, 228, 246, 258, 411, 901, 1437
BpmI CTGGAG 1 cut(s) 1112
Bpu10I CCTNAGC 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 1445
BpuMI CCSGG 2 cut(s) 874, 1194
BsaAI YACGTR 1 cut(s) 172
BsaBI GATNNNNATC 2 cut(s) 312, 1583
BsaWI WCCGGW 1 cut(s) 683
BsaXI ACNNNNNCTCC 4 cut(s) 35, 65, 74, 104
Bsc4I CCNNNNNNNGG 3 cut(s) 873, 892, 1681
Bse1I ACTGG 1 cut(s) 524
Bse8I GATNNNNATC 2 cut(s) 312, 1583
BseBI CCWGG 1 cut(s) 1203
BseGI GGATG 4 cut(s) 187, 766, 1311, 1774
BseJI GATNNNNATC 2 cut(s) 312, 1583
BseLI CCNNNNNNNGG 3 cut(s) 873, 892, 1681
BseMII CTCAG 3 cut(s) 6, 498, 1607
BseNI ACTGG 1 cut(s) 524
BseRI GAGGAG 9 cut(s) 43, 53, 56, 59, 62, 79, 206, 209, 212
BseSI GKGCMC 1 cut(s) 958
BseXI GCAGC 4 cut(s) 138, 869, 1084, 1396
BseYI CCCAGC 1 cut(s) 1810
Bsh1236I CGCG 2 cut(s) 336, 560
BshFI GGCC 3 cut(s) 712, 1057, 1288
BshNI GGYRCC 1 cut(s) 1318
BsiHKAI GWGCWC 1 cut(s) 806
BsiSI CCGG 3 cut(s) 684, 874, 1194
BslFI GGGAC 2 cut(s) 856, 1780
BslI CCNNNNNNNGG 3 cut(s) 873, 892, 1681
BsmAI GTCTC 1 cut(s) 91
BsmBI CGTCTC 1 cut(s) 91
BsmFI GGGAC 2 cut(s) 856, 1780
BsnI GGCC 3 cut(s) 712, 1057, 1288
Bsp1286I GDGCHC 2 cut(s) 806, 958
Bsp68I TCGCGA 1 cut(s) 560
BspACI CCGC 2 cut(s) 336, 1058
BspANI GGCC 3 cut(s) 712, 1057, 1288
BspCNI CTCAG 3 cut(s) 7, 497, 1608
BspFNI CGCG 2 cut(s) 336, 560
BspHI TCATGA 2 cut(s) 310, 1084
BspLI GGNNCC 1 cut(s) 1320
BspMAI CTGCAG 2 cut(s) 296, 836
BspPI GGATC 4 cut(s) 796, 848, 1184, 1526
BspQI GCTCTTC 2 cut(s) 41, 811
BspT107I GGYRCC 1 cut(s) 1318
BspTI CTTAAG 1 cut(s) 983
BsrI ACTGG 1 cut(s) 524
Bst2UI CCWGG 1 cut(s) 1203
Bst4CI ACNGT 3 cut(s) 538, 784, 907
Bst6I CTCTTC 7 cut(s) 41, 80, 116, 183, 811, 1545, 1638
BstAFI CTTAAG 1 cut(s) 983
BstBAI YACGTR 1 cut(s) 172
BstDEI CTNAG 5 cut(s) 15, 484, 798, 940, 1616
BstF5I GGATG 4 cut(s) 187, 766, 1311, 1774
BstFNI CGCG 2 cut(s) 336, 560
BstHHI GCGC 1 cut(s) 422
BstMAI GTCTC 1 cut(s) 91
BstMWI GCNNNNNNNGC 5 cut(s) 20, 1106, 1203, 1225, 1273
BstNI CCWGG 1 cut(s) 1203
BstSCI CCNGG 3 cut(s) 872, 1192, 1201
BstSFI CTRYAG 3 cut(s) 292, 594, 832
BstSLI GKGCMC 1 cut(s) 958
BstUI CGCG 2 cut(s) 336, 560
BstV1I GCAGC 4 cut(s) 138, 869, 1084, 1396
BstV2I GAAGAC 7 cut(s) 171, 228, 246, 258, 411, 901, 1437
BstX2I RGATCY 2 cut(s) 853, 1591
BstYI RGATCY 2 cut(s) 853, 1591
BsuI GTATCC 1 cut(s) 1507
BsuRI GGCC 3 cut(s) 712, 1057, 1288
BtrI CACGTC 1 cut(s) 1181
BtsCI GGATG 4 cut(s) 187, 766, 1311, 1774
BtsIMutI CAGTG 2 cut(s) 531, 846
BtuMI TCGCGA 1 cut(s) 560
CaiI CAGNNNCTG 1 cut(s) 491
CciI TCATGA 2 cut(s) 310, 1084
CfoI GCGC 1 cut(s) 422
Cfr13I GGNCC 2 cut(s) 576, 710
CseI GACGC 1 cut(s) 682
Csp6I GTAC 1 cut(s) 1067
CviQI GTAC 1 cut(s) 1067
DdeI CTNAG 5 cut(s) 15, 484, 798, 940, 1616
DraIII CACNNNGTG 1 cut(s) 1181
Eam1104I CTCTTC 7 cut(s) 41, 80, 116, 183, 811, 1545, 1638
EarI CTCTTC 7 cut(s) 41, 80, 116, 183, 811, 1545, 1638
Ecl136II GAGCTC 1 cut(s) 804
Eco24I GRGCYC 1 cut(s) 806
Eco47I GGWCC 1 cut(s) 576
Eco53kI GAGCTC 1 cut(s) 804
Eco57I CTGAAG 2 cut(s) 359, 870
EcoICRI GAGCTC 1 cut(s) 804
EcoRII CCWGG 1 cut(s) 1201
EcoT38I GRGCYC 1 cut(s) 806
Esp3I CGTCTC 1 cut(s) 91
FalI AAGNNNNNCTT 2 cut(s) 1613, 1645
FaqI GGGAC 2 cut(s) 856, 1780
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 6 cut(s) 127, 337, 883, 1058, 1098, 1410
FokI GGATG 4 cut(s) 194, 773, 1318, 1781
FriOI GRGCYC 1 cut(s) 806
Fsp4HI GCNGC 6 cut(s) 127, 337, 883, 1058, 1098, 1410
FspBI CTAG 3 cut(s) 602, 1302, 1689
GlaI GCGC 1 cut(s) 421
GluI GCNGC 6 cut(s) 127, 337, 883, 1058, 1098, 1410
GsaI CCCAGC 1 cut(s) 1814
GsuI CTGGAG 1 cut(s) 1112
HaeIII GGCC 3 cut(s) 712, 1057, 1288
HapII CCGG 3 cut(s) 684, 874, 1194
HgaI GACGC 1 cut(s) 682
HhaI GCGC 1 cut(s) 422
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HindIII AAGCTT 1 cut(s) 1627
HpaII CCGG 3 cut(s) 684, 874, 1194
HphI GGTGA 6 cut(s) 275, 465, 635, 698, 785, 1376
Hpy166II GTNNAC 2 cut(s) 579, 706
Hpy188III TCNNGA 6 cut(s) 311, 559, 746, 1085, 1176, 1766
Hpy8I GTNNAC 2 cut(s) 579, 706
Hpy99I CGWCG 2 cut(s) 1182, 1602
HpyAV CCTTC 4 cut(s) 67, 383, 1376, 1627
HpyCH4III ACNGT 3 cut(s) 538, 784, 907
HpyCH4IV ACGT 7 cut(s) 84, 171, 1069, 1104, 1180, 1445, 1600
HpyCH4V TGCA 6 cut(s) 294, 466, 834, 1109, 1412, 1718
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 1106, 1203, 1225, 1273
HpyF3I CTNAG 5 cut(s) 15, 484, 798, 940, 1616
HpySE526I ACGT 7 cut(s) 84, 171, 1069, 1104, 1180, 1445, 1600
HspAI GCGC 1 cut(s) 420
Ksp22I TGATCA 1 cut(s) 307
LguI GCTCTTC 2 cut(s) 41, 811
LmnI GCTCC 1 cut(s) 969
Lsp1109I GCAGC 4 cut(s) 138, 869, 1084, 1396
LweI GCATC 2 cut(s) 388, 1181
MaeI CTAG 3 cut(s) 602, 1302, 1689
MaeII ACGT 7 cut(s) 84, 171, 1069, 1104, 1180, 1445, 1600
MaeIII GTNAC 8 cut(s) 281, 471, 611, 623, 686, 773, 1070, 1326
MfeI CAATTG 1 cut(s) 719
MflI RGATCY 2 cut(s) 853, 1591
MhlI GDGCHC 2 cut(s) 806, 958
MlyI GAGTC 3 cut(s) 479, 770, 905
MmeI TCCRAC 1 cut(s) 766
MroXI GAANNNNTTC 1 cut(s) 1522
MseI TTAA 7 cut(s) 350, 434, 828, 984, 1121, 1137, 1418
MspA1I CMGCKG 2 cut(s) 14, 1409
MspCI CTTAAG 1 cut(s) 983
MspI CCGG 3 cut(s) 684, 874, 1194
MspR9I CCNGG 3 cut(s) 874, 1194, 1203
MunI CAATTG 1 cut(s) 719
MvaI CCWGG 1 cut(s) 1203
MvnI CGCG 2 cut(s) 336, 560
MwoI GCNNNNNNNGC 5 cut(s) 20, 1106, 1203, 1225, 1273
NciI CCSGG 2 cut(s) 874, 1194
NlaIV GGNNCC 1 cut(s) 1320
NmuCI GTSAC 7 cut(s) 281, 471, 611, 623, 686, 773, 1070
NruI TCGCGA 1 cut(s) 560
PagI TCATGA 2 cut(s) 310, 1084
PciSI GCTCTTC 2 cut(s) 41, 811
PdmI GAANNNNTTC 1 cut(s) 1522
PfeI GAWTC 8 cut(s) 344, 458, 488, 839, 977, 1035, 1437, 1790
PfoI TCCNGGA 1 cut(s) 872
PkrI GCNGC 6 cut(s) 128, 338, 884, 1059, 1099, 1411
PleI GAGTC 3 cut(s) 478, 770, 904
PpsI GAGTC 3 cut(s) 478, 770, 904
Ppu21I YACGTR 1 cut(s) 172
Psp124BI GAGCTC 1 cut(s) 806
Psp1406I AACGTT 1 cut(s) 1445
Psp6I CCWGG 1 cut(s) 1201
PspFI CCCAGC 1 cut(s) 1810
PspGI CCWGG 1 cut(s) 1201
PspN4I GGNNCC 1 cut(s) 1320
PspPI GGNCC 2 cut(s) 576, 710
PstI CTGCAG 2 cut(s) 296, 836
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 2 cut(s) 853, 1591
PvuII CAGCTG 2 cut(s) 14, 1409
RruI TCGCGA 1 cut(s) 560
RsaI GTAC 1 cut(s) 1068
RsaNI GTAC 1 cut(s) 1067
SacI GAGCTC 1 cut(s) 806
SapI GCTCTTC 2 cut(s) 41, 811
SaqAI TTAA 7 cut(s) 350, 434, 828, 984, 1121, 1137, 1418
SatI GCNGC 6 cut(s) 127, 337, 883, 1058, 1098, 1410
Sau96I GGNCC 2 cut(s) 576, 710
SchI GAGTC 3 cut(s) 479, 770, 905
ScrFI CCNGG 3 cut(s) 874, 1194, 1203
SduI GDGCHC 2 cut(s) 806, 958
SfaNI GCATC 2 cut(s) 388, 1181
SfcI CTRYAG 3 cut(s) 292, 594, 832
SinI GGWCC 1 cut(s) 576
SmlI CTYRAG 2 cut(s) 983, 1460
SmoI CTYRAG 2 cut(s) 983, 1460
SsiI CCGC 2 cut(s) 336, 1058
SspMI CTAG 3 cut(s) 602, 1302, 1689
SstI GAGCTC 1 cut(s) 806
StyD4I CCNGG 3 cut(s) 872, 1192, 1201
TaaI ACNGT 3 cut(s) 538, 784, 907
TaiI ACGT 7 cut(s) 87, 174, 1072, 1107, 1183, 1448, 1603
TaqI TCGA 2 cut(s) 568, 1597
TaqII GACCGA 1 cut(s) 1721
TauI GCSGC 2 cut(s) 339, 1060
TfiI GAWTC 8 cut(s) 344, 458, 488, 839, 977, 1035, 1437, 1790
Tru1I TTAA 7 cut(s) 350, 434, 828, 984, 1121, 1137, 1418
Tru9I TTAA 7 cut(s) 350, 434, 828, 984, 1121, 1137, 1418
TscAI CASTG 2 cut(s) 531, 853
TseFI GTSAC 7 cut(s) 281, 471, 611, 623, 686, 773, 1070
TseI GCWGC 4 cut(s) 126, 882, 1097, 1409
Tsp45I GTSAC 7 cut(s) 281, 471, 611, 623, 686, 773, 1070
TspGWI ACGGA 1 cut(s) 1449
TspRI CASTG 2 cut(s) 531, 853
Vha464I CTTAAG 1 cut(s) 983
VpaK11BI GGWCC 1 cut(s) 576
XapI RAATTY 5 cut(s) 397, 765, 1113, 1153, 1398
XmnI GAANNNNTTC 1 cut(s) 1522
XspI CTAG 3 cut(s) 602, 1302, 1689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.