MD06G1216400.v1.1

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008073
Physical Location & Seq
Reverse (-)
34853098 .. 34856479
3382 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1216400.v1.1.491

Sequence Viewer

Length: 1782 bp
ATGCCATGTTCAGCTGAGGAAGCACTAGTTAGGCTCTTCTACAACACCTCCTCCTCCTTCCACCTCCTCCTCCTCTTCCTCTACCTCTCCTCCACTTTTCTTTTCAAACTCTTCTACTTTGTCGGCAGCAACCCTATTTTCCCAAGAAACCAGAATGGATACGAAGACTATGTGTTTTCGGACGAAGAAGAAGAGGAGGAGCAGGAGGAAGAAGAAGACAACGAACAAAGGCAGTTTCATGTTCCTGGTCACCAATACTGCAGTACCATAAATGATCATGATCTGGTGGCTGACATCATTCACGGCGGCGAATCACTGATGTTTTTGCCTAACAATAACCTTCAGCATCATCATCAAAAAAGTTTTTCCGAAGACCAATTCATTAGCGCACGGGAGAGCTTAATAATTGAAGACCGCCCCGATGAATCTTTGCATGAGTCATCACAAGGTTCAGAATCTGAACATGACGATCAAGGTGCGGAGGAAATCCCCGTGAAAGATACAGTTTCAGTTCTCAATTCTGTTGCGAAGGATCGAGTGTGGCCCACATCTCCGATAACTATAGAGCTACATAAAAGTGACAAAAATGGTGACGGTAATTGTGATGAACATAATACTGAAATCAATAGGGCAGATCAGACGCATTTATCCAGTGACGAAAATTTTGTGGTTTTCGGGCCATCACAATTGAAGAACAAGAAGTTCCAAGTTCAAGACAATAAAGAGGATGAAATTTTTGAGGACTCAACTGTTGGATCAACTTCTAAGAGCTCTTCTGATTGGAGAAGCTCAATTAATTGCAGGGATTCTGCCACTGAAGATCCTTTTTCTTCCTCGTCCCGGCGAAGCTGCCCCAAATGGGAGTCTTACACAGTCTTCCAAAAGTATGATGAAGAAATGATGTTCCTAGACAGAATTAGTGCCCAAAAGCTCCATGAAACAGAATCACTTAAGTCTATTCAAGTCTGTCCAAGATCAATTTCGGAAAGGATTGTTCATAGAATCTCTACGGCAACAAAAAGGCTGCCTGATGTACGTCACAACCCTTATCATGAGCTGGAGGCTGCTTATGTTGCACAAATTTGCTTAACATGGGAAGCACTTAATTGGAACTACAAGAATTTTGAGCAAAAAAGGGCATCACGCCATGAGTTTGATCCCGGCGTTCCTGGCGAATTAGCACAGAAATTTCAGCAATTTCAAGTGCTTCTACAAAGATATGTAGAGAATGAGCCTTATGAGCAAGGCAGGAGGCCAGAGATTTATGCTAGGATGAGGCTTTTGGCACCAAAGTTACTTCAAGTGCCAGAATATCGAGAAGATGATGACCATCAAAAGGATGAAGGTTTTGGCTCAAAAATTTCGTCAGCTGCATTTTTAATGATAATGGAAGACGGAATCCGAACGTTCATGGATTTTCTCAAGACTGATAAGGAAAAACCCTGCCAGAAATTAGCATCCATGTTCAAACGAAAACGAAAAGGTTCAGTTGATCCCACTCTTCTCCATCTGATGAAGAAAGTTAATCAAAAAAAGAAGATGAAAATCAAAGATCTTCGACGTTCCCACAAATGCCTGAGAAAGCGAAGGTTGAAGGTGGAGGAAGAGATGGAGATACTGATGGGTCTGATTGACCTAAAACTGGTATCTAGGGTTTTGAGAATGACCGACCTAAATGCAGAACAATTGCATTGGTGCGAAGCAAAGATGAGCAAAGTGCGAATTCTGGATGGGAGAAAATACCAAAGAGATTCCTCCCCACTTTTCTTCCCAGCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

594

Amino Acids

69.22

Weight (kDa)

5.67

Isoelectric Point (pI)

57.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 351 - 591 1.9e-89 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1285
AciI CCGC 3 cut(s) 306, 415, 479
AclI AACGTT 1 cut(s) 1406
AclWI GGATC 5 cut(s) 540, 763, 815, 1151, 1487
AcsI RAATTY 7 cut(s) 661, 732, 1080, 1120, 1187, 1359, 1722
AcuI CTGAAG 2 cut(s) 326, 837
AfaI GTAC 2 cut(s) 265, 1035
AfiI CCNNNNNNNGG 4 cut(s) 840, 859, 1336, 1642
AflII CTTAAG 1 cut(s) 950
AgsI TTSAA 9 cut(s) 106, 410, 691, 713, 962, 1202, 1301, 1468, 1594
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 2 cut(s) 244, 1168
AjuI GAANNNNNNNTTGG 2 cut(s) 964, 996
AluBI AGCT 9 cut(s) 14, 399, 568, 771, 789, 849, 931, 1057, 1370
AluI AGCT 9 cut(s) 14, 399, 568, 771, 789, 849, 931, 1057, 1370
Alw21I GWGCWC 1 cut(s) 773
AlwI GGATC 5 cut(s) 540, 763, 815, 1151, 1487
AlwNI CAGNNNCTG 1 cut(s) 458
AoxI GGCC 3 cut(s) 542, 677, 1253
ApeKI GCWGC 5 cut(s) 126, 849, 1024, 1064, 1370
ApoI RAATTY 7 cut(s) 661, 732, 1080, 1120, 1187, 1359, 1722
ArsI GACNNNNNNTTYG 4 cut(s) 647, 679, 1349, 1381
AseI ATTAAT 1 cut(s) 795
Asp700I GAANNNNTTC 1 cut(s) 1483
AspLEI GCGC 1 cut(s) 389
AspS9I GGNCC 2 cut(s) 543, 677
AsuC2I CCSGG 2 cut(s) 841, 1161
AsuHPI GGTGA 2 cut(s) 242, 602
BaeGI GKGCMC 1 cut(s) 925
BaeI ACNNNNGTAYC 2 cut(s) 247, 280
BanI GGYRCC 1 cut(s) 1285
BanII GRGCYC 1 cut(s) 773
BbsI GAAGAC 6 cut(s) 171, 222, 378, 417, 868, 1398
Bbv12I GWGCWC 1 cut(s) 773
BbvCI CCTCAGC 1 cut(s) 15
BbvI GCAGC 5 cut(s) 138, 836, 1011, 1051, 1357
BccI CCATC 6 cut(s) 688, 1338, 1515, 1603, 1615, 1724
BceAI ACGGC 2 cut(s) 319, 1026
BcgI CGANNNNNNTGC 4 cut(s) 458, 492, 1658, 1692
BciT130I CCWGG 2 cut(s) 246, 1170
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 2 cut(s) 841, 1161
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 4 cut(s) 26, 908, 1269, 1650
BfmI CTRYAG 2 cut(s) 259, 561
BfrI CTTAAG 1 cut(s) 950
BfuI GTATCC 1 cut(s) 152
BglII AGATCT 1 cut(s) 1552
BisI GCNGC 6 cut(s) 127, 307, 850, 1025, 1065, 1371
BlsI GCNGC 6 cut(s) 128, 308, 851, 1026, 1066, 1372
Bme1390I CCNGG 4 cut(s) 246, 841, 1161, 1170
BmgT120I GGNCC 2 cut(s) 543, 677
BmiI GGNNCC 1 cut(s) 1287
BmrFI CCNGG 4 cut(s) 246, 841, 1161, 1170
BmsI GCATC 3 cut(s) 355, 1148, 1466
BpiI GAAGAC 6 cut(s) 171, 222, 378, 417, 868, 1398
BpmI CTGGAG 1 cut(s) 1079
Bpu10I CCTNAGC 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 1406
BpuMI CCSGG 2 cut(s) 841, 1161
BsaBI GATNNNNATC 3 cut(s) 279, 1329, 1544
BsaXI ACNNNNNCTCC 6 cut(s) 32, 35, 62, 65, 74, 104
Bsc4I CCNNNNNNNGG 4 cut(s) 840, 859, 1336, 1642
Bse1I ACTGG 2 cut(s) 651, 1647
Bse8I GATNNNNATC 3 cut(s) 279, 1329, 1544
BseBI CCWGG 2 cut(s) 246, 1170
BseGI GGATG 5 cut(s) 733, 1278, 1345, 1457, 1735
BseJI GATNNNNATC 3 cut(s) 279, 1329, 1544
BseLI CCNNNNNNNGG 4 cut(s) 840, 859, 1336, 1642
BseMII CTCAG 2 cut(s) 6, 1568
BseNI ACTGG 2 cut(s) 651, 1647
BseRI GAGGAG 8 cut(s) 40, 43, 56, 59, 62, 79, 209, 212
BseSI GKGCMC 1 cut(s) 925
BseXI GCAGC 5 cut(s) 138, 836, 1011, 1051, 1357
BseYI CCCAGC 1 cut(s) 1771
BshFI GGCC 3 cut(s) 544, 679, 1255
BshNI GGYRCC 1 cut(s) 1285
BsiHKAI GWGCWC 1 cut(s) 773
BsiSI CCGG 2 cut(s) 841, 1161
BslFI GGGAC 1 cut(s) 823
BslI CCNNNNNNNGG 4 cut(s) 840, 859, 1336, 1642
BsmFI GGGAC 1 cut(s) 823
BsnI GGCC 3 cut(s) 544, 679, 1255
Bsp1286I GDGCHC 2 cut(s) 773, 925
BspACI CCGC 3 cut(s) 306, 415, 479
BspANI GGCC 3 cut(s) 544, 679, 1255
BspCNI CTCAG 2 cut(s) 7, 1569
BspHI TCATGA 2 cut(s) 277, 1051
BspLI GGNNCC 1 cut(s) 1287
BspMAI CTGCAG 1 cut(s) 263
BspPI GGATC 5 cut(s) 540, 763, 815, 1151, 1487
BspQI GCTCTTC 2 cut(s) 41, 778
BspT107I GGYRCC 1 cut(s) 1285
BspTI CTTAAG 1 cut(s) 950
BsrI ACTGG 2 cut(s) 651, 1647
Bst2UI CCWGG 2 cut(s) 246, 1170
Bst4CI ACNGT 4 cut(s) 505, 596, 751, 874
Bst6I CTCTTC 7 cut(s) 41, 80, 116, 186, 778, 1506, 1599
BstAFI CTTAAG 1 cut(s) 950
BstDEI CTNAG 3 cut(s) 15, 765, 1577
BstEII GGTNACC 1 cut(s) 248
BstF5I GGATG 5 cut(s) 733, 1278, 1345, 1457, 1735
BstHHI GCGC 1 cut(s) 389
BstMWI GCNNNNNNNGC 4 cut(s) 20, 1073, 1170, 1240
BstNI CCWGG 2 cut(s) 246, 1170
BstPI GGTNACC 1 cut(s) 248
BstSCI CCNGG 4 cut(s) 244, 839, 1159, 1168
BstSFI CTRYAG 2 cut(s) 259, 561
BstSLI GKGCMC 1 cut(s) 925
BstV1I GCAGC 5 cut(s) 138, 836, 1011, 1051, 1357
BstV2I GAAGAC 6 cut(s) 171, 222, 378, 417, 868, 1398
BstX2I RGATCY 2 cut(s) 820, 1552
BstYI RGATCY 2 cut(s) 820, 1552
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 3 cut(s) 544, 679, 1255
BtsCI GGATG 5 cut(s) 733, 1278, 1345, 1457, 1735
BtsIMutI CAGTG 3 cut(s) 314, 658, 813
CaiI CAGNNNCTG 1 cut(s) 458
CciI TCATGA 2 cut(s) 277, 1051
CfoI GCGC 1 cut(s) 389
Cfr13I GGNCC 2 cut(s) 543, 677
CseI GACGC 1 cut(s) 649
Csp6I GTAC 2 cut(s) 264, 1034
CviQI GTAC 2 cut(s) 264, 1034
DdeI CTNAG 3 cut(s) 15, 765, 1577
Eam1104I CTCTTC 7 cut(s) 41, 80, 116, 186, 778, 1506, 1599
EarI CTCTTC 7 cut(s) 41, 80, 116, 186, 778, 1506, 1599
Ecl136II GAGCTC 1 cut(s) 771
Eco24I GRGCYC 1 cut(s) 773
Eco53kI GAGCTC 1 cut(s) 771
Eco57I CTGAAG 2 cut(s) 326, 837
Eco91I GGTNACC 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 771
EcoO65I GGTNACC 1 cut(s) 248
EcoRI GAATTC 1 cut(s) 1722
EcoRII CCWGG 2 cut(s) 244, 1168
EcoT38I GRGCYC 1 cut(s) 773
FaqI GGGAC 1 cut(s) 823
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 6 cut(s) 127, 307, 850, 1025, 1065, 1371
FokI GGATG 5 cut(s) 740, 1285, 1352, 1444, 1742
FriOI GRGCYC 1 cut(s) 773
Fsp4HI GCNGC 6 cut(s) 127, 307, 850, 1025, 1065, 1371
FspBI CTAG 4 cut(s) 26, 908, 1269, 1650
GlaI GCGC 1 cut(s) 388
GluI GCNGC 6 cut(s) 127, 307, 850, 1025, 1065, 1371
GsaI CCCAGC 1 cut(s) 1775
GsuI CTGGAG 1 cut(s) 1079
HaeIII GGCC 3 cut(s) 544, 679, 1255
HapII CCGG 2 cut(s) 841, 1161
HgaI GACGC 1 cut(s) 649
HhaI GCGC 1 cut(s) 389
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HpaII CCGG 2 cut(s) 841, 1161
HphI GGTGA 2 cut(s) 242, 602
Hpy188III TCNNGA 6 cut(s) 278, 713, 1052, 1316, 1423, 1727
Hpy99I CGWCG 1 cut(s) 1563
HpyAV CCTTC 6 cut(s) 67, 350, 523, 1337, 1581, 1588
HpyCH4III ACNGT 4 cut(s) 505, 596, 751, 874
HpyCH4IV ACGT 3 cut(s) 1036, 1406, 1561
HpyCH4V TGCA 7 cut(s) 261, 433, 801, 1076, 1373, 1679, 1690
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 1073, 1170, 1240
HpyF3I CTNAG 3 cut(s) 15, 765, 1577
HpySE526I ACGT 3 cut(s) 1036, 1406, 1561
HspAI GCGC 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 274
LguI GCTCTTC 2 cut(s) 41, 778
LmnI GCTCC 2 cut(s) 199, 936
Lsp1109I GCAGC 5 cut(s) 138, 836, 1011, 1051, 1357
LweI GCATC 3 cut(s) 355, 1148, 1466
MaeI CTAG 4 cut(s) 26, 908, 1269, 1650
MaeII ACGT 3 cut(s) 1036, 1406, 1561
MaeIII GTNAC 6 cut(s) 248, 578, 590, 653, 1037, 1293
MfeI CAATTG 2 cut(s) 686, 1685
MflI RGATCY 2 cut(s) 820, 1552
MhlI GDGCHC 2 cut(s) 773, 925
MlyI GAGTC 3 cut(s) 446, 737, 872
MmeI TCCRAC 1 cut(s) 733
MroXI GAANNNNTTC 1 cut(s) 1483
MseI TTAA 7 cut(s) 401, 795, 951, 1088, 1104, 1379, 1524
MslI CAYNNNNRTG 1 cut(s) 576
MspA1I CMGCKG 2 cut(s) 14, 1370
MspCI CTTAAG 1 cut(s) 950
MspI CCGG 2 cut(s) 841, 1161
MspR9I CCNGG 4 cut(s) 246, 841, 1161, 1170
MunI CAATTG 2 cut(s) 686, 1685
MvaI CCWGG 2 cut(s) 246, 1170
MwoI GCNNNNNNNGC 4 cut(s) 20, 1073, 1170, 1240
NciI CCSGG 2 cut(s) 841, 1161
NlaIV GGNNCC 1 cut(s) 1287
NmuCI GTSAC 5 cut(s) 248, 578, 590, 653, 1037
PagI TCATGA 2 cut(s) 277, 1051
PciSI GCTCTTC 2 cut(s) 41, 778
PdmI GAANNNNTTC 1 cut(s) 1483
PfeI GAWTC 8 cut(s) 311, 425, 455, 806, 944, 1002, 1398, 1751
PkrI GCNGC 6 cut(s) 128, 308, 851, 1026, 1066, 1372
PleI GAGTC 3 cut(s) 445, 737, 871
PpsI GAGTC 3 cut(s) 445, 737, 871
PshBI ATTAAT 1 cut(s) 795
Psp124BI GAGCTC 1 cut(s) 773
Psp1406I AACGTT 1 cut(s) 1406
Psp6I CCWGG 2 cut(s) 244, 1168
PspEI GGTNACC 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 1771
PspGI CCWGG 2 cut(s) 244, 1168
PspN4I GGNNCC 1 cut(s) 1287
PspPI GGNCC 2 cut(s) 543, 677
PstI CTGCAG 1 cut(s) 263
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 2 cut(s) 820, 1552
PvuII CAGCTG 2 cut(s) 14, 1370
RsaI GTAC 2 cut(s) 265, 1035
RsaNI GTAC 2 cut(s) 264, 1034
RseI CAYNNNNRTG 1 cut(s) 576
SacI GAGCTC 1 cut(s) 773
SapI GCTCTTC 2 cut(s) 41, 778
SaqAI TTAA 7 cut(s) 401, 795, 951, 1088, 1104, 1379, 1524
SatI GCNGC 6 cut(s) 127, 307, 850, 1025, 1065, 1371
Sau96I GGNCC 2 cut(s) 543, 677
SchI GAGTC 3 cut(s) 446, 737, 872
ScrFI CCNGG 4 cut(s) 246, 841, 1161, 1170
SduI GDGCHC 2 cut(s) 773, 925
SfaNI GCATC 3 cut(s) 355, 1148, 1466
SfcI CTRYAG 2 cut(s) 259, 561
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 2 cut(s) 950, 1421
SmoI CTYRAG 2 cut(s) 950, 1421
SpeI ACTAGT 1 cut(s) 25
SsiI CCGC 3 cut(s) 306, 415, 479
SspMI CTAG 4 cut(s) 26, 908, 1269, 1650
SstI GAGCTC 1 cut(s) 773
StyD4I CCNGG 4 cut(s) 244, 839, 1159, 1168
TaaI ACNGT 4 cut(s) 505, 596, 751, 874
TaiI ACGT 3 cut(s) 1039, 1409, 1564
TaqI TCGA 3 cut(s) 535, 1315, 1558
TaqII GACCGA 1 cut(s) 1682
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 8 cut(s) 311, 425, 455, 806, 944, 1002, 1398, 1751
Tru1I TTAA 7 cut(s) 401, 795, 951, 1088, 1104, 1379, 1524
Tru9I TTAA 7 cut(s) 401, 795, 951, 1088, 1104, 1379, 1524
TscAI CASTG 3 cut(s) 321, 658, 820
TseFI GTSAC 5 cut(s) 248, 578, 590, 653, 1037
TseI GCWGC 5 cut(s) 126, 849, 1024, 1064, 1370
Tsp45I GTSAC 5 cut(s) 248, 578, 590, 653, 1037
TspGWI ACGGA 1 cut(s) 1410
TspRI CASTG 3 cut(s) 321, 658, 820
Vha464I CTTAAG 1 cut(s) 950
VspI ATTAAT 1 cut(s) 795
XapI RAATTY 7 cut(s) 661, 732, 1080, 1120, 1187, 1359, 1722
XmnI GAANNNNTTC 1 cut(s) 1483
XspI CTAG 4 cut(s) 26, 908, 1269, 1650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.