AT1G74460

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014174
Physical Location & Seq
Reverse (-)
27987890 .. 27989865
1976 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74460.1

Sequence Viewer

Length: 1101 bp
ATGAAGTTCTGCGCGATATTCGTGTTATTCATCGTTCTTGCAATCAACGGCTACGATTGCAAGATAGTCCAATTTATATTTGGAGATTCATTGTCTGATGTAGGCAACAATAAGAATCTACCTAGGAGTTTAGCGACGGCGAATCTGCCCTTTTACGGTATTGATTTTGGCAATGGTTTGCCTAATGGAAGGTTCACTAATGGCCGCACTGTTTCAGACATTATAGGTGATAAAATTGGATTGCCAAGACCAGTAGCCTTCTTGGATCCATCAATGAATGAAGATGTAATACTTGAAAATGGAGTGAACTATGCTTCAGGAGGTGGTGGAATCTTGAACGAAACTGGTGGTTATTTCATCCAAAGATTTTCGCTGTGGAAGCAAATAGAATTATTCCAAGGGACACAAGATGTAGTTGTGGCAAAGATAGGGAAAAAAGAAGCAGACAAGTTCTTTCAAGATGCTCGATACGTCGTCGCTTTAGGCAGCAATGACTTCATTAACAACTATTTGATGCCTGTCTATAGCGACTCTTGGAAATACAATGATCAAACCTTCGTCGACTACTTAATGGAAACACTTGAATCTCAACTCAAGGTGTTACATAGTTTAGGAGCGAGGAAGCTAATGGTGTTTGGTTTAGGACCAATGGGTTGTATCCCACTGCAAAGAGCTCTAAGCCTGGATGGAAATTGTCAAAACAAAGCGAGTAATCTTGCTAAGAGATTCAACAAAGCTGCCACCACAATGCTCCTAGATCTCGAGACCAAGCTCCCTAACGCAAGCTACAGGTTTGGTGAAGCCTATGACCTCGTCAACGACGTCATCACTAACCCAAAAAAATACGGGTTTGATAACTCGGATTCGCCGTGTTGCTCGTTCTACAGAATCCGGCCTGCGCTAACGTGTATTCCAGCGTCGACATTGTGTAAAGACAGAAGTAAGTATGTGTTTTGGGATGAGTATCATCCCACCGATAAGGCTAACGAACTCGTCGCTAATATACTCATCAAAAGATTTGACTTCATGCGTGCCGATGACGGTATCTCCCATGCTCCATCTCCCGCGCCGGATATTTCTCCTTCTTCCGACAATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

40.7

Weight (kDa)

5.73

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 25 - 335 3.5e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74460
fragaria_vesca FvH4_5g14860
malus_domestica MD06G1194000.v1.1 MD14G1201200.v1.1
prunus_persica Prupe.5G194400_v2.0.a1
pyrus_communis pycom06g17190 pycom14g16670
rosa_chinensis RchiOBHm_Chr7g0181521
rosa_laevigata RLG00000005186
rosa_multiflora Rmu_sc0004621.1_g000032
rosa_roxburghii Rroxscaffold_3G00271930
rosa_rugosa Rorug06G0442000
rosa_samantha Rh7AG044100 Rh7BG043200 Rh7CG045000 Rh7DG043700
rosa_wichuraiana Rw7G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 825
AccI GTMKAC 2 cut(s) 561, 920
AccII CGCG 2 cut(s) 14, 1067
AciI CCGC 2 cut(s) 205, 1065
AclWI GGATC 2 cut(s) 260, 273
AcoI YGGCCR 1 cut(s) 202
AcuI CTGAAG 1 cut(s) 300
AcyI GRCGYC 1 cut(s) 822
AfiI CCNNNNNNNGG 1 cut(s) 155
AflIII ACRYGT 1 cut(s) 905
AgsI TTSAA 5 cut(s) 296, 337, 458, 584, 730
AjnI CCWGG 1 cut(s) 681
AluBI AGCT 5 cut(s) 625, 674, 737, 772, 786
AluI AGCT 5 cut(s) 625, 674, 737, 772, 786
Alw21I GWGCWC 1 cut(s) 676
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 2 cut(s) 260, 273
Ama87I CYCGRG 1 cut(s) 761
AoxI GGCC 2 cut(s) 202, 893
ApeKI GCWGC 2 cut(s) 486, 737
AspA2I CCTAGG 1 cut(s) 122
AspLEI GCGC 3 cut(s) 14, 901, 1069
AspS9I GGNCC 1 cut(s) 644
AsuHPI GGTGA 2 cut(s) 239, 809
AvaI CYCGRG 1 cut(s) 761
AvaII GGWCC 1 cut(s) 644
AvrII CCTAGG 1 cut(s) 122
BamHI GGATCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 676
BarI GAAGNNNNNNTAC 2 cut(s) 273, 305
Bbv12I GWGCWC 1 cut(s) 676
BbvI GCAGC 2 cut(s) 498, 724
BccI CCATC 3 cut(s) 277, 680, 1066
BceAI ACGGC 3 cut(s) 64, 153, 853
BciT130I CCWGG 1 cut(s) 683
BciVI GTATCC 1 cut(s) 668
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 1 cut(s) 758
BfaI CTAG 2 cut(s) 123, 755
BfmI CTRYAG 3 cut(s) 523, 787, 883
BfuI GTATCC 1 cut(s) 668
BglII AGATCT 1 cut(s) 757
BisI GCNGC 3 cut(s) 205, 487, 738
BlnI CCTAGG 1 cut(s) 122
BlsI GCNGC 3 cut(s) 206, 488, 739
Bme1390I CCNGG 1 cut(s) 683
Bme18I GGWCC 1 cut(s) 644
BmeT110I CYCGRG 1 cut(s) 761
BmgT120I GGNCC 1 cut(s) 644
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 683
BmsI GCATC 2 cut(s) 451, 504
BpuEI CTTGAG 1 cut(s) 578
BsaBI GATNNNNATC 1 cut(s) 963
BsaHI GRCGYC 1 cut(s) 822
BsaI GGTCTC 1 cut(s) 758
BsaJI CCNNGG 2 cut(s) 122, 397
BsaXI ACNNNNNCTCC 2 cut(s) 1031, 1061
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse1I ACTGG 2 cut(s) 251, 349
Bse3DI GCAATG 2 cut(s) 178, 496
Bse8I GATNNNNATC 1 cut(s) 963
BseBI CCWGG 1 cut(s) 683
BseDI CCNNGG 2 cut(s) 122, 397
BseGI GGATG 4 cut(s) 357, 691, 964, 967
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 2 cut(s) 178, 496
BseNI ACTGG 2 cut(s) 251, 349
BseXI GCAGC 2 cut(s) 498, 724
Bsh1236I CGCG 2 cut(s) 14, 1067
BshFI GGCC 2 cut(s) 204, 895
BsiHKAI GWGCWC 1 cut(s) 676
BsiHKCI CYCGRG 1 cut(s) 761
BsiSI CCGG 2 cut(s) 892, 1070
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 758
BsmFI GGGAC 1 cut(s) 415
BsnI GGCC 2 cut(s) 204, 895
Bso31I GGTCTC 1 cut(s) 758
BsoBI CYCGRG 1 cut(s) 761
Bsp1286I GDGCHC 1 cut(s) 676
Bsp143I GATC 3 cut(s) 265, 547, 757
BspACI CCGC 2 cut(s) 205, 1065
BspANI GGCC 2 cut(s) 204, 895
BspFNI CGCG 2 cut(s) 14, 1067
BspLI GGNNCC 1 cut(s) 267
BspPI GGATC 2 cut(s) 260, 273
BspTNI GGTCTC 1 cut(s) 758
BsrDI GCAATG 2 cut(s) 178, 496
BsrI ACTGG 2 cut(s) 251, 349
BssECI CCNNGG 2 cut(s) 122, 397
BssMI GATC 3 cut(s) 265, 547, 757
BssNI GRCGYC 1 cut(s) 822
BssT1I CCWWGG 2 cut(s) 122, 397
Bst2UI CCWGG 1 cut(s) 683
Bst4CI ACNGT 3 cut(s) 158, 211, 1043
BstACI GRCGYC 1 cut(s) 822
BstC8I GCNNGC 3 cut(s) 784, 897, 1032
BstDEI CTNAG 2 cut(s) 677, 720
BstF5I GGATG 4 cut(s) 357, 691, 964, 967
BstFNI CGCG 2 cut(s) 14, 1067
BstHHI GCGC 3 cut(s) 14, 901, 1069
BstKTI GATC 3 cut(s) 268, 550, 760
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 3 cut(s) 265, 547, 757
BstMWI GCNNNNNNNGC 2 cut(s) 57, 379
BstNI CCWGG 1 cut(s) 683
BstSCI CCNGG 1 cut(s) 681
BstSFI CTRYAG 3 cut(s) 523, 787, 883
BstUI CGCG 2 cut(s) 14, 1067
BstV1I GCAGC 2 cut(s) 498, 724
BstX2I RGATCY 2 cut(s) 265, 757
BstYI RGATCY 2 cut(s) 265, 757
BsuI GTATCC 1 cut(s) 668
BsuRI GGCC 2 cut(s) 204, 895
BtsCI GGATG 4 cut(s) 357, 691, 964, 967
BtsI GCAGTG 1 cut(s) 662
BtsIMutI CAGTG 2 cut(s) 207, 662
Cac8I GCNNGC 3 cut(s) 784, 897, 1032
CfoI GCGC 3 cut(s) 14, 901, 1069
Cfr13I GGNCC 1 cut(s) 644
CseI GACGC 1 cut(s) 906
CviAII CATG 2 cut(s) 1027, 1052
DdeI CTNAG 2 cut(s) 677, 720
DpnI GATC 3 cut(s) 267, 549, 759
DpnII GATC 3 cut(s) 265, 547, 757
EaeI YGGCCR 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 674
Eco130I CCWWGG 2 cut(s) 122, 397
Eco24I GRGCYC 1 cut(s) 676
Eco31I GGTCTC 1 cut(s) 758
Eco47I GGWCC 1 cut(s) 644
Eco53kI GAGCTC 1 cut(s) 674
Eco57I CTGAAG 1 cut(s) 300
Eco88I CYCGRG 1 cut(s) 761
EcoICRI GAGCTC 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 681
EcoT14I CCWWGG 2 cut(s) 122, 397
EcoT38I GRGCYC 1 cut(s) 676
ErhI CCWWGG 2 cut(s) 122, 397
FaeI CATG 2 cut(s) 1030, 1055
FaqI GGGAC 1 cut(s) 415
FatI CATG 2 cut(s) 1026, 1051
FauI CCCGC 1 cut(s) 1072
FbaI TGATCA 1 cut(s) 547
FblI GTMKAC 2 cut(s) 561, 920
Fnu4HI GCNGC 3 cut(s) 205, 487, 738
FokI GGATG 4 cut(s) 344, 698, 954, 971
FriOI GRGCYC 1 cut(s) 676
Fsp4HI GCNGC 3 cut(s) 205, 487, 738
FspBI CTAG 2 cut(s) 123, 755
GlaI GCGC 3 cut(s) 13, 900, 1068
GluI GCNGC 3 cut(s) 205, 487, 738
HaeIII GGCC 2 cut(s) 204, 895
HapII CCGG 2 cut(s) 892, 1070
HgaI GACGC 1 cut(s) 906
HhaI GCGC 3 cut(s) 14, 901, 1069
Hin1I GRCGYC 1 cut(s) 822
Hin1II CATG 2 cut(s) 1030, 1055
Hin6I GCGC 3 cut(s) 12, 899, 1067
HinP1I GCGC 3 cut(s) 12, 899, 1067
HincII GTYRAC 3 cut(s) 562, 817, 921
HindII GTYRAC 3 cut(s) 562, 817, 921
HinfI GANTC 9 cut(s) 86, 115, 142, 330, 530, 584, 726, 863, 888
HpaII CCGG 2 cut(s) 892, 1070
HphI GGTGA 2 cut(s) 239, 809
Hpy166II GTNNAC 5 cut(s) 195, 307, 562, 817, 921
Hpy188I TCNGA 4 cut(s) 97, 217, 862, 1090
Hpy188III TCNNGA 5 cut(s) 318, 334, 458, 761, 763
Hpy8I GTNNAC 5 cut(s) 195, 307, 562, 817, 921
Hpy99I CGWCG 7 cut(s) 139, 476, 479, 563, 824, 922, 998
HpyAV CCTTC 4 cut(s) 183, 268, 565, 1092
HpyCH4III ACNGT 3 cut(s) 158, 211, 1043
HpyCH4IV ACGT 3 cut(s) 471, 822, 905
HpyCH4V TGCA 3 cut(s) 41, 60, 667
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 379
HpyF3I CTNAG 2 cut(s) 677, 720
HpySE526I ACGT 3 cut(s) 471, 822, 905
Hsp92I GRCGYC 1 cut(s) 822
Hsp92II CATG 2 cut(s) 1030, 1055
HspAI GCGC 3 cut(s) 12, 899, 1067
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 3 cut(s) 265, 547, 757
LmnI GCTCC 4 cut(s) 614, 756, 777, 1060
Lsp1109I GCAGC 2 cut(s) 498, 724
LweI GCATC 2 cut(s) 451, 504
MaeI CTAG 2 cut(s) 123, 755
MaeII ACGT 3 cut(s) 471, 822, 905
MaeIII GTNAC 1 cut(s) 600
MalI GATC 3 cut(s) 267, 549, 759
MboI GATC 3 cut(s) 265, 547, 757
MboII GAAGA 2 cut(s) 293, 1077
MflI RGATCY 2 cut(s) 265, 757
MhlI GDGCHC 1 cut(s) 676
MluCI AATT 4 cut(s) 71, 234, 389, 691
MlyI GAGTC 1 cut(s) 524
MnlI CCTC 3 cut(s) 314, 612, 821
MseI TTAA 2 cut(s) 501, 569
MslI CAYNNNNRTG 1 cut(s) 746
MspI CCGG 2 cut(s) 892, 1070
MspR9I CCNGG 1 cut(s) 683
MvaI CCWGG 1 cut(s) 683
MvnI CGCG 2 cut(s) 14, 1067
MwoI GCNNNNNNNGC 2 cut(s) 57, 379
NdeII GATC 3 cut(s) 265, 547, 757
NlaIII CATG 2 cut(s) 1030, 1055
NlaIV GGNNCC 1 cut(s) 267
PaeR7I CTCGAG 1 cut(s) 761
PcsI WCGNNNNNNNCGW 2 cut(s) 819, 866
PfeI GAWTC 8 cut(s) 86, 115, 142, 330, 584, 726, 863, 888
PflFI GACNNNGTC 1 cut(s) 812
PkrI GCNGC 3 cut(s) 206, 488, 739
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
Psp124BI GAGCTC 1 cut(s) 676
Psp6I CCWGG 1 cut(s) 681
PspGI CCWGG 1 cut(s) 681
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 1 cut(s) 644
PsuI RGATCY 2 cut(s) 265, 757
PsyI GACNNNGTC 1 cut(s) 812
RseI CAYNNNNRTG 1 cut(s) 746
SacI GAGCTC 1 cut(s) 676
SalI GTCGAC 2 cut(s) 560, 919
SaqAI TTAA 2 cut(s) 501, 569
SatI GCNGC 3 cut(s) 205, 487, 738
Sau3AI GATC 3 cut(s) 265, 547, 757
Sau96I GGNCC 1 cut(s) 644
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 1 cut(s) 683
SduI GDGCHC 1 cut(s) 676
SfaNI GCATC 2 cut(s) 451, 504
SfcI CTRYAG 3 cut(s) 523, 787, 883
Sfr274I CTCGAG 1 cut(s) 761
SinI GGWCC 1 cut(s) 644
SlaI CTCGAG 1 cut(s) 761
SmiMI CAYNNNNRTG 1 cut(s) 746
SmlI CTYRAG 2 cut(s) 593, 761
SmoI CTYRAG 2 cut(s) 593, 761
Sse9I AATT 4 cut(s) 71, 234, 389, 691
SsiI CCGC 2 cut(s) 205, 1065
SspMI CTAG 2 cut(s) 123, 755
SstI GAGCTC 1 cut(s) 676
StyD4I CCNGG 1 cut(s) 681
StyI CCWWGG 2 cut(s) 122, 397
TaaI ACNGT 3 cut(s) 158, 211, 1043
TaiI ACGT 3 cut(s) 474, 825, 908
TaqI TCGA 4 cut(s) 466, 561, 762, 920
TasI AATT 4 cut(s) 71, 234, 389, 691
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 8 cut(s) 86, 115, 142, 330, 584, 726, 863, 888
Tru1I TTAA 2 cut(s) 501, 569
Tru9I TTAA 2 cut(s) 501, 569
TscAI CASTG 2 cut(s) 214, 669
TseI GCWGC 2 cut(s) 486, 737
TspDTI ATGAA 8 cut(s) 17, 19, 78, 290, 294, 346, 487, 1015
TspRI CASTG 2 cut(s) 214, 669
Tth111I GACNNNGTC 1 cut(s) 812
VpaK11BI GGWCC 1 cut(s) 644
XcmI CCANNNNNNNNNTGG 1 cut(s) 77
XhoI CTCGAG 1 cut(s) 761
XmaJI CCTAGG 1 cut(s) 122
XmiI GTMKAC 2 cut(s) 561, 920
XspI CTAG 2 cut(s) 123, 755
ZraI GACGTC 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.