FvH4_5g14860

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014174
Physical Location & Seq
Reverse (-)
8427767 .. 8430000
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14860.t1

Sequence Viewer

Length: 1107 bp
ATGAAGCTCAATGTGGGAGTAGCAGTGTTGGTTGTGACCATCTTTGCCATCCTGATCGATGGTTACAGCTGCAAAGTGGTGCAGTTCATATTTGGCGATTCTCTCTCTGATGTTGGAAACAACAAGTACTTGAGCAAGAGCCTCGCCCAAGCAAGCCTCCCCTGGTATGGTATTGATTTTGGCAATGGTTTGCCTAATGGGAGATTCTCTAATGGTCGAACAGTTGCTGATATTATAGGTGATAACATGGGTCTCCCGAGGCCACCTGCATTTCTAGACCCATCTTTAACCGAAGATATGATACTCCAAAATGGAGTGAACTATGCTTCTGGAGGTGGTGGAATCTTGAATGAAACTGGTGGCTTATTTATTCAAAGGTTTTCACTCTCCAAGCAGATCGAGCTGTTTATAGGGACACAAACATTGATAAAGAGCAAAATTGGCAAAGAGGCAGCAGACAAGTATTTCCAAGGAGCTCGTTACGTTGTCGCTTTAGGCAGCAATGACTTCATCAACAATTATTTGATGCCAGTCTATAGCGATTCTTGGAACTACAACGATCAAAGCTTCATCGACTACTTGATGGAAACACTGAAGGCACAACTTCAGCTGTTGCATGCTTTAGGGGCAAGGCAGCTGATGGTGTTTGGTTTAGGACCAATGGGTTGTATCCCACTACAGAGGGTCCTAAGTACATCTGGGGATTGTGTAGATAGAACAAACAAATTAGCTCTCAGCTTCAACAAAGCTGGAAGCAAATTAATGGACGATTTGAACGCCAAACTTCCTAACGCCAGCTACAGATTTGGTGATGCTTATGATGTTGTCAATAATGTGATCAGCAATCCAAGCAAATATGGATTCAGTAATTCAGACTCACCATGCTGCTCATTCGGAAGAATAAGACCGGCTCTTACATGTGTGCCTGCATCAGTACTATGCAAAGACAGAAGTAAATATGTATTCTGGGATGAGTACCACCCGTCAGATAGCGCTAACCAGCTTATTGCCACTGAGCTCATCAAGAAATTCGGTTTCACGCGTGTCGATCAGGGCAATGCTCCTTCCTCAGCACCAGCAATTGCTCCATCCCCAGATCAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.05

Weight (kDa)

6.18

Isoelectric Point (pI)

31.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 339 7.3e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74460
fragaria_vesca FvH4_5g14860
malus_domestica MD06G1194000.v1.1 MD14G1201200.v1.1
prunus_persica Prupe.5G194400_v2.0.a1
pyrus_communis pycom06g17190 pycom14g16670
rosa_chinensis RchiOBHm_Chr7g0181521
rosa_laevigata RLG00000005186
rosa_multiflora Rmu_sc0004621.1_g000032
rosa_roxburghii Rroxscaffold_3G00271930
rosa_rugosa Rorug06G0442000
rosa_samantha Rh7AG044100 Rh7BG043200 Rh7CG045000 Rh7DG043700
rosa_wichuraiana Rw7G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 274
Acc36I ACCTGC 1 cut(s) 274
AccII CGCG 1 cut(s) 1042
AcsI RAATTY 1 cut(s) 1028
AcuI CTGAAG 2 cut(s) 590, 614
AfaI GTAC 4 cut(s) 128, 694, 936, 977
AfeI AGCGCT 1 cut(s) 994
AfiI CCNNNNNNNGG 2 cut(s) 167, 1099
AflIII ACRYGT 2 cut(s) 917, 1040
AgsI TTSAA 4 cut(s) 349, 374, 742, 775
AjnI CCWGG 1 cut(s) 161
Alw21I GWGCWC 2 cut(s) 478, 1020
Alw26I GTCTC 1 cut(s) 257
AlwNI CAGNNNCTG 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 256
Aor51HI AGCGCT 1 cut(s) 994
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 5 cut(s) 69, 452, 498, 634, 885
ApoI RAATTY 1 cut(s) 1028
AseI ATTAAT 1 cut(s) 761
AspLEI GCGC 1 cut(s) 995
AspS9I GGNCC 2 cut(s) 656, 685
AsuHPI GGTGA 3 cut(s) 251, 821, 870
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 2 cut(s) 656, 685
BanII GRGCYC 2 cut(s) 478, 1020
BarI GAAGNNNNNNTAC 2 cut(s) 285, 317
Bbv12I GWGCWC 2 cut(s) 478, 1020
BbvCI CCTCAGC 1 cut(s) 1069
BbvI GCAGC 5 cut(s) 56, 464, 510, 646, 872
BccI CCATC 7 cut(s) 47, 53, 56, 289, 577, 634, 1096
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 1 cut(s) 163
BciVI GTATCC 1 cut(s) 680
BclI TGATCA 1 cut(s) 837
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 3 cut(s) 535, 677, 799
BfoI RGCGCY 1 cut(s) 996
BfuAI ACCTGC 1 cut(s) 274
BfuI GTATCC 1 cut(s) 680
BisI GCNGC 5 cut(s) 70, 453, 499, 635, 886
BlsI GCNGC 5 cut(s) 71, 454, 500, 636, 887
BmcAI AGTACT 2 cut(s) 128, 936
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 2 cut(s) 656, 685
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 2 cut(s) 656, 685
BmiI GGNNCC 1 cut(s) 686
BmrFI CCNGG 1 cut(s) 163
BmsI GCATC 3 cut(s) 516, 802, 938
BpmI CTGGAG 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 1069
BpuEI CTTGAG 1 cut(s) 151
Bsa29I ATCGAT 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 161, 257, 469
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 1099
Bse118I RCCGGY 1 cut(s) 907
Bse1I ACTGG 2 cut(s) 361, 530
Bse3DI GCAATG 3 cut(s) 190, 508, 1063
BseBI CCWGG 1 cut(s) 163
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 3 cut(s) 161, 257, 469
BseGI GGATG 3 cut(s) 48, 976, 1088
BseLI CCNNNNNNNGG 2 cut(s) 167, 1099
BseMI GCAATG 3 cut(s) 190, 508, 1063
BseMII CTCAG 3 cut(s) 748, 1005, 1083
BseNI ACTGG 2 cut(s) 361, 530
BseXI GCAGC 5 cut(s) 56, 464, 510, 646, 872
BsgI GTGCAG 1 cut(s) 101
Bsh1236I CGCG 1 cut(s) 1042
BshFI GGCC 1 cut(s) 262
BshVI ATCGAT 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 478, 1020
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 1 cut(s) 908
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 2 cut(s) 167, 1099
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 427
BsnI GGCC 1 cut(s) 262
Bso31I GGTCTC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 478, 1020
Bsp143I GATC 6 cut(s) 54, 396, 559, 837, 1048, 1096
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 3 cut(s) 747, 1006, 1082
BspDI ATCGAT 1 cut(s) 57
BspFNI CGCG 1 cut(s) 1042
BspLI GGNNCC 1 cut(s) 686
BspMI ACCTGC 1 cut(s) 274
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 3 cut(s) 190, 508, 1063
BsrFI RCCGGY 1 cut(s) 907
BsrI ACTGG 2 cut(s) 361, 530
BssAI RCCGGY 1 cut(s) 907
BssECI CCNNGG 3 cut(s) 161, 257, 469
BssMI GATC 6 cut(s) 54, 396, 559, 837, 1048, 1096
BssT1I CCWWGG 1 cut(s) 469
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 1 cut(s) 223
BstC8I GCNNGC 4 cut(s) 154, 618, 796, 927
BstDEI CTNAG 4 cut(s) 689, 734, 1014, 1069
BstF5I GGATG 3 cut(s) 48, 976, 1088
BstFNI CGCG 1 cut(s) 1042
BstH2I RGCGCY 1 cut(s) 996
BstHHI GCGC 1 cut(s) 995
BstKTI GATC 6 cut(s) 57, 399, 562, 840, 1051, 1099
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 6 cut(s) 54, 396, 559, 837, 1048, 1096
BstMWI GCNNNNNNNGC 4 cut(s) 400, 441, 626, 849
BstNI CCWGG 1 cut(s) 163
BstNSI RCATGY 2 cut(s) 620, 921
BstSCI CCNGG 1 cut(s) 161
BstSFI CTRYAG 3 cut(s) 535, 677, 799
BstUI CGCG 1 cut(s) 1042
BstV1I GCAGC 5 cut(s) 56, 464, 510, 646, 872
Bsu15I ATCGAT 1 cut(s) 57
BsuI GTATCC 1 cut(s) 680
BsuRI GGCC 1 cut(s) 262
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 3 cut(s) 48, 976, 1088
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 3 cut(s) 30, 590, 1011
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 4 cut(s) 154, 618, 796, 927
CaiI CAGNNNCTG 1 cut(s) 227
CfoI GCGC 1 cut(s) 995
Cfr10I RCCGGY 1 cut(s) 907
Cfr13I GGNCC 2 cut(s) 656, 685
ClaI ATCGAT 1 cut(s) 57
Csp6I GTAC 4 cut(s) 127, 693, 935, 976
CviAII CATG 4 cut(s) 247, 617, 882, 918
CviQI GTAC 4 cut(s) 127, 693, 935, 976
DdeI CTNAG 4 cut(s) 689, 734, 1014, 1069
DpnI GATC 6 cut(s) 56, 398, 561, 839, 1050, 1098
DpnII GATC 6 cut(s) 54, 396, 559, 837, 1048, 1096
Ecl136II GAGCTC 2 cut(s) 476, 1018
Eco130I CCWWGG 1 cut(s) 469
Eco24I GRGCYC 2 cut(s) 478, 1020
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 2 cut(s) 656, 685
Eco47III AGCGCT 1 cut(s) 994
Eco53kI GAGCTC 2 cut(s) 476, 1018
Eco57I CTGAAG 2 cut(s) 590, 614
Eco88I CYCGRG 1 cut(s) 256
EcoICRI GAGCTC 2 cut(s) 476, 1018
EcoO109I RGGNCCY 1 cut(s) 685
EcoRII CCWGG 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 469
EcoT38I GRGCYC 2 cut(s) 478, 1020
ErhI CCWWGG 1 cut(s) 469
FaeI CATG 4 cut(s) 250, 620, 885, 921
FaqI GGGAC 1 cut(s) 427
FatI CATG 4 cut(s) 246, 616, 881, 917
FbaI TGATCA 1 cut(s) 837
Fnu4HI GCNGC 5 cut(s) 70, 453, 499, 635, 886
FokI GGATG 3 cut(s) 35, 983, 1075
FriOI GRGCYC 2 cut(s) 478, 1020
Fsp4HI GCNGC 5 cut(s) 70, 453, 499, 635, 886
FspBI CTAG 1 cut(s) 275
GlaI GCGC 1 cut(s) 994
GluI GCNGC 5 cut(s) 70, 453, 499, 635, 886
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 996
HaeIII GGCC 1 cut(s) 262
HapII CCGG 1 cut(s) 908
HhaI GCGC 1 cut(s) 995
Hin1II CATG 4 cut(s) 250, 620, 885, 921
Hin6I GCGC 1 cut(s) 993
HinP1I GCGC 1 cut(s) 993
HindIII AAGCTT 1 cut(s) 565
HinfI GANTC 6 cut(s) 98, 204, 342, 542, 861, 875
HpaII CCGG 1 cut(s) 908
HphI GGTGA 3 cut(s) 251, 821, 870
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 4 cut(s) 109, 874, 896, 988
Hpy188III TCNNGA 7 cut(s) 52, 256, 275, 330, 346, 1024, 1100
Hpy8I GTNNAC 1 cut(s) 319
HpyAV CCTTC 2 cut(s) 589, 1074
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 6 cut(s) 72, 82, 269, 616, 929, 942
HpyF10VI GCNNNNNNNGC 4 cut(s) 400, 441, 626, 849
HpyF3I CTNAG 4 cut(s) 689, 734, 1014, 1069
HpySE526I ACGT 1 cut(s) 483
Hsp92II CATG 4 cut(s) 250, 620, 885, 921
HspAI GCGC 1 cut(s) 993
Ksp22I TGATCA 1 cut(s) 837
Kzo9I GATC 6 cut(s) 54, 396, 559, 837, 1048, 1096
LmnI GCTCC 3 cut(s) 473, 1066, 1090
Lsp1109I GCAGC 5 cut(s) 56, 464, 510, 646, 872
LweI GCATC 3 cut(s) 516, 802, 938
MaeI CTAG 1 cut(s) 275
MaeII ACGT 1 cut(s) 483
MaeIII GTNAC 3 cut(s) 34, 62, 479
MalI GATC 6 cut(s) 56, 398, 561, 839, 1050, 1098
MboI GATC 6 cut(s) 54, 396, 559, 837, 1048, 1096
MboII GAAGA 2 cut(s) 305, 909
MfeI CAATTG 1 cut(s) 1080
MhlI GDGCHC 2 cut(s) 478, 1020
MluCI AATT 7 cut(s) 438, 517, 725, 758, 868, 1028, 1080
MluI ACGCGT 1 cut(s) 1040
MlyI GAGTC 1 cut(s) 869
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 7 cut(s) 152, 167, 252, 326, 442, 675, 1078
MseI TTAA 2 cut(s) 287, 761
MspA1I CMGCKG 3 cut(s) 69, 610, 637
MspI CCGG 1 cut(s) 908
MspR9I CCNGG 1 cut(s) 163
MunI CAATTG 1 cut(s) 1080
MvaI CCWGG 1 cut(s) 163
MvnI CGCG 1 cut(s) 1042
MwoI GCNNNNNNNGC 4 cut(s) 400, 441, 626, 849
NdeII GATC 6 cut(s) 54, 396, 559, 837, 1048, 1096
NlaIII CATG 4 cut(s) 250, 620, 885, 921
NlaIV GGNNCC 1 cut(s) 686
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 2 cut(s) 620, 921
PaeI GCATGC 1 cut(s) 620
PaqCI CACCTGC 1 cut(s) 274
PciI ACATGT 1 cut(s) 917
PfeI GAWTC 5 cut(s) 98, 204, 342, 542, 861
PkrI GCNGC 5 cut(s) 71, 454, 500, 636, 887
PleI GAGTC 1 cut(s) 869
PpsI GAGTC 1 cut(s) 869
PpuMI RGGWCCY 1 cut(s) 685
PscI ACATGT 1 cut(s) 917
PshBI ATTAAT 1 cut(s) 761
Psp124BI GAGCTC 2 cut(s) 478, 1020
Psp5II RGGWCCY 1 cut(s) 685
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 1 cut(s) 686
PspPI GGNCC 2 cut(s) 656, 685
PspPPI RGGWCCY 1 cut(s) 685
PstNI CAGNNNCTG 1 cut(s) 227
PvuII CAGCTG 3 cut(s) 69, 610, 637
RsaI GTAC 4 cut(s) 128, 694, 936, 977
RsaNI GTAC 4 cut(s) 127, 693, 935, 976
SacI GAGCTC 2 cut(s) 478, 1020
SaqAI TTAA 2 cut(s) 287, 761
SatI GCNGC 5 cut(s) 70, 453, 499, 635, 886
Sau3AI GATC 6 cut(s) 54, 396, 559, 837, 1048, 1096
Sau96I GGNCC 2 cut(s) 656, 685
ScaI AGTACT 2 cut(s) 128, 936
SchI GAGTC 1 cut(s) 869
ScrFI CCNGG 1 cut(s) 163
SduI GDGCHC 2 cut(s) 478, 1020
SfaNI GCATC 3 cut(s) 516, 802, 938
SfcI CTRYAG 3 cut(s) 535, 677, 799
SinI GGWCC 2 cut(s) 656, 685
SmlI CTYRAG 1 cut(s) 130
SmoI CTYRAG 1 cut(s) 130
SphI GCATGC 1 cut(s) 620
Sse9I AATT 7 cut(s) 438, 517, 725, 758, 868, 1028, 1080
SspMI CTAG 1 cut(s) 275
SstI GAGCTC 2 cut(s) 478, 1020
StyD4I CCNGG 1 cut(s) 161
StyI CCWWGG 1 cut(s) 469
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 486
TaqI TCGA 5 cut(s) 57, 217, 399, 573, 1047
TasI AATT 7 cut(s) 438, 517, 725, 758, 868, 1028, 1080
TatI WGTACW 3 cut(s) 126, 692, 934
TfiI GAWTC 5 cut(s) 98, 204, 342, 542, 861
Tru1I TTAA 2 cut(s) 287, 761
Tru9I TTAA 2 cut(s) 287, 761
TscAI CASTG 3 cut(s) 30, 597, 1018
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 5 cut(s) 69, 452, 498, 634, 885
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 5 cut(s) 17, 76, 366, 499, 559
TspRI CASTG 3 cut(s) 30, 597, 1018
VpaK11BI GGWCC 2 cut(s) 656, 685
VspI ATTAAT 1 cut(s) 761
XapI RAATTY 1 cut(s) 1028
XbaI TCTAGA 1 cut(s) 274
XceI RCATGY 2 cut(s) 620, 921
XspI CTAG 1 cut(s) 275
ZrmI AGTACT 2 cut(s) 128, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.