Rh7DG043700

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0014174
Physical Location & Seq
Forward (+)
3327700 .. 3330102
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG043700.1

Sequence Viewer

Length: 861 bp
ATGGGTCTCCCAAGGCCCCCTGCCTATCTAGACCCATCTTTAACCGAAGACATGATACTCGAAAATGGAGTGAACTATGCTTCTGGAGGAGGTGGAATCTTGAATGAAACTGGTGCCTTATTTATTCAAAGGCTTTCACTCTACAAGCAAATCGAGCTTTTTGAAGGGACACAAACATTGATAAGGAGCAAAATTGGCAAAGTGGCAGCAGACAAGTTCTTCCAAGGAGCTCGTTACGTGGTTGCTTTAGGCAGCAATGACTTCATCAACAATTACTTGATGCCGGTCTATAGCGATTCCTGGAATTACAACGATCAAAGCTTCATCGACTACTTGATGGAAACACTAAAGGCACAACTTCAGCTATTGCATACTTTAGGGGCAAGGCAGCTGATGGTGTTTGGTTTAGGACCAATGGGTTGTATTCCACTACAGAGGGTTCTAAGTACGTCTGGGGATTGCGTAGACAGAGCAAACAACTTAGCTCTCAGCTTCAACAAAGCTGGAAGCAAGCTAATGGACAATTTGAACGCCAAACTTCCCAACTCCAGCTATAGATTTGGTGATGCTTATGATGTGGTCAATAATATCATCATCAATCCAAGCAAATATGGATTCAGTAACTCAGACTCACCATGCTGCTCATTCGGAAAAATAAGACCGGCTCTAACATGTGTGCCTGCATCAGTACTGTGCAAAGACAGAAGCAAATATCTGTTCTGGGATGAGTACCACCCATCGGATAGCGCTAACCAATTTATTGCTAATGAGCTCATCAAGAAATTCGGTTTCACGCGTGTCGATCAAGGCGATGCTCCTTCCTCAGCACCTGCCATTGCTCCATCACCAGATCAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

286

Amino Acids

31.45

Weight (kDa)

5.74

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 21 - 258 3.9e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74460
fragaria_vesca FvH4_5g14860
malus_domestica MD06G1194000.v1.1 MD14G1201200.v1.1
prunus_persica Prupe.5G194400_v2.0.a1
pyrus_communis pycom06g17190 pycom14g16670
rosa_chinensis RchiOBHm_Chr7g0181521
rosa_laevigata RLG00000005186
rosa_multiflora Rmu_sc0004621.1_g000032
rosa_roxburghii Rroxscaffold_3G00271930
rosa_rugosa Rorug06G0442000
rosa_samantha Rh7AG044100 Rh7BG043200 Rh7CG045000 Rh7DG043700
rosa_wichuraiana Rw7G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 838
Acc36I ACCTGC 1 cut(s) 838
AccB1I GGYRCC 1 cut(s) 113
AccI GTMKAC 1 cut(s) 465
AccII CGCG 1 cut(s) 796
AcsI RAATTY 1 cut(s) 782
AcuI CTGAAG 1 cut(s) 344
AfaI GTAC 3 cut(s) 448, 690, 731
AfeI AGCGCT 1 cut(s) 748
AfiI CCNNNNNNNGG 1 cut(s) 739
AflIII ACRYGT 2 cut(s) 671, 794
AgsI TTSAA 5 cut(s) 103, 128, 164, 496, 529
AjnI CCWGG 1 cut(s) 299
Alw21I GWGCWC 2 cut(s) 232, 774
Alw26I GTCTC 1 cut(s) 11
AlwNI CAGNNNCTG 1 cut(s) 830
Aor51HI AGCGCT 1 cut(s) 748
AoxI GGCC 1 cut(s) 14
ApeKI GCWGC 4 cut(s) 206, 252, 388, 639
ApoI RAATTY 1 cut(s) 782
AspLEI GCGC 1 cut(s) 749
AspS9I GGNCC 2 cut(s) 15, 410
AsuHPI GGTGA 3 cut(s) 575, 624, 837
AvaII GGWCC 1 cut(s) 410
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 2 cut(s) 232, 774
BarI GAAGNNNNNNTAC 2 cut(s) 39, 71
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 2 cut(s) 232, 774
BbvCI CCTCAGC 1 cut(s) 823
BbvI GCAGC 4 cut(s) 218, 264, 400, 626
BccI CCATC 5 cut(s) 43, 331, 388, 745, 850
BciT130I CCWGG 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 3 cut(s) 289, 431, 553
BfoI RGCGCY 1 cut(s) 750
BfuAI ACCTGC 1 cut(s) 838
BisI GCNGC 4 cut(s) 207, 253, 389, 640
BlsI GCNGC 4 cut(s) 208, 254, 390, 641
BmcAI AGTACT 1 cut(s) 690
Bme1390I CCNGG 1 cut(s) 301
Bme18I GGWCC 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 15, 410
BmiI GGNNCC 2 cut(s) 17, 115
BmrFI CCNGG 1 cut(s) 301
BmsI GCATC 4 cut(s) 270, 556, 692, 802
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 2 cut(s) 105, 532
Bpu10I CCTNAGC 1 cut(s) 823
BsaAI YACGTR 1 cut(s) 238
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 11, 223
Bsc4I CCNNNNNNNGG 1 cut(s) 739
Bse118I RCCGGY 2 cut(s) 283, 661
Bse1I ACTGG 1 cut(s) 115
Bse3DI GCAATG 2 cut(s) 262, 834
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 2 cut(s) 11, 223
BseGI GGATG 1 cut(s) 730
BseLI CCNNNNNNNGG 1 cut(s) 739
BseMI GCAATG 2 cut(s) 262, 834
BseMII CTCAG 3 cut(s) 502, 639, 837
BseNI ACTGG 1 cut(s) 115
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 4 cut(s) 218, 264, 400, 626
Bsh1236I CGCG 1 cut(s) 796
BshFI GGCC 1 cut(s) 16
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 2 cut(s) 232, 774
BsiSI CCGG 2 cut(s) 284, 662
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 739
BsmAI GTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 1 cut(s) 16
Bso31I GGTCTC 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 232, 774
Bsp143I GATC 3 cut(s) 313, 802, 850
BspANI GGCC 1 cut(s) 16
BspCNI CTCAG 3 cut(s) 501, 638, 836
BspFNI CGCG 1 cut(s) 796
BspLI GGNNCC 2 cut(s) 17, 115
BspMI ACCTGC 1 cut(s) 838
BspT107I GGYRCC 1 cut(s) 113
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 2 cut(s) 262, 834
BsrFI RCCGGY 2 cut(s) 283, 661
BsrI ACTGG 1 cut(s) 115
BssAI RCCGGY 2 cut(s) 283, 661
BssECI CCNNGG 2 cut(s) 11, 223
BssMI GATC 3 cut(s) 313, 802, 850
BssT1I CCWWGG 2 cut(s) 11, 223
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 1 cut(s) 693
BstBAI YACGTR 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 512, 681
BstDEI CTNAG 5 cut(s) 443, 481, 488, 625, 823
BstF5I GGATG 1 cut(s) 730
BstFNI CGCG 1 cut(s) 796
BstH2I RGCGCY 1 cut(s) 750
BstHHI GCGC 1 cut(s) 749
BstKTI GATC 3 cut(s) 316, 805, 853
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 3 cut(s) 313, 802, 850
BstMWI GCNNNNNNNGC 2 cut(s) 154, 195
BstNI CCWGG 1 cut(s) 301
BstNSI RCATGY 1 cut(s) 675
BstSCI CCNGG 1 cut(s) 299
BstSFI CTRYAG 3 cut(s) 289, 431, 553
BstUI CGCG 1 cut(s) 796
BstV1I GCAGC 4 cut(s) 218, 264, 400, 626
BstV2I GAAGAC 1 cut(s) 54
BsuRI GGCC 1 cut(s) 16
BtgZI GCGATG 1 cut(s) 825
BtsCI GGATG 1 cut(s) 730
BveI ACCTGC 1 cut(s) 838
Cac8I GCNNGC 2 cut(s) 512, 681
CaiI CAGNNNCTG 1 cut(s) 830
CfoI GCGC 1 cut(s) 749
Cfr10I RCCGGY 2 cut(s) 283, 661
Cfr13I GGNCC 2 cut(s) 15, 410
Csp6I GTAC 3 cut(s) 447, 689, 730
CviAII CATG 3 cut(s) 52, 636, 672
CviQI GTAC 3 cut(s) 447, 689, 730
DdeI CTNAG 5 cut(s) 443, 481, 488, 625, 823
DpnI GATC 3 cut(s) 315, 804, 852
DpnII GATC 3 cut(s) 313, 802, 850
Ecl136II GAGCTC 2 cut(s) 230, 772
Eco130I CCWWGG 2 cut(s) 11, 223
Eco24I GRGCYC 2 cut(s) 232, 774
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 410
Eco47III AGCGCT 1 cut(s) 748
Eco53kI GAGCTC 2 cut(s) 230, 772
Eco57I CTGAAG 1 cut(s) 344
EcoICRI GAGCTC 2 cut(s) 230, 772
EcoO109I RGGNCCY 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 299
EcoT14I CCWWGG 2 cut(s) 11, 223
EcoT38I GRGCYC 2 cut(s) 232, 774
ErhI CCWWGG 2 cut(s) 11, 223
FaeI CATG 3 cut(s) 55, 639, 675
FaiI YATR 9 cut(s) 53, 78, 291, 372, 555, 573, 612, 637, 673
FaqI GGGAC 1 cut(s) 181
FatI CATG 3 cut(s) 51, 635, 671
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 4 cut(s) 207, 253, 389, 640
FokI GGATG 1 cut(s) 737
FriOI GRGCYC 2 cut(s) 232, 774
Fsp4HI GCNGC 4 cut(s) 207, 253, 389, 640
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 748
GluI GCNGC 4 cut(s) 207, 253, 389, 640
GsuI CTGGAG 2 cut(s) 105, 532
HaeII RGCGCY 1 cut(s) 750
HaeIII GGCC 1 cut(s) 16
HapII CCGG 2 cut(s) 284, 662
HhaI GCGC 1 cut(s) 749
Hin1II CATG 3 cut(s) 55, 639, 675
Hin6I GCGC 1 cut(s) 747
HinP1I GCGC 1 cut(s) 747
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 4 cut(s) 96, 296, 615, 629
HpaII CCGG 2 cut(s) 284, 662
HphI GGTGA 3 cut(s) 575, 624, 837
Hpy166II GTNNAC 2 cut(s) 73, 466
Hpy188I TCNGA 4 cut(s) 628, 650, 742, 855
Hpy188III TCNNGA 4 cut(s) 29, 84, 100, 778
Hpy8I GTNNAC 2 cut(s) 73, 466
HpyAV CCTTC 2 cut(s) 158, 828
HpyCH4III ACNGT 1 cut(s) 693
HpyCH4IV ACGT 2 cut(s) 237, 449
HpyCH4V TGCA 3 cut(s) 370, 683, 696
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 195
HpyF3I CTNAG 5 cut(s) 443, 481, 488, 625, 823
HpySE526I ACGT 2 cut(s) 237, 449
Hsp92II CATG 3 cut(s) 55, 639, 675
HspAI GCGC 1 cut(s) 747
Kzo9I GATC 3 cut(s) 313, 802, 850
LmnI GCTCC 4 cut(s) 186, 227, 820, 844
Lsp1109I GCAGC 4 cut(s) 218, 264, 400, 626
LweI GCATC 4 cut(s) 270, 556, 692, 802
MaeI CTAG 1 cut(s) 29
MaeII ACGT 2 cut(s) 237, 449
MaeIII GTNAC 2 cut(s) 233, 620
MalI GATC 3 cut(s) 315, 804, 852
MboI GATC 3 cut(s) 313, 802, 850
MboII GAAGA 2 cut(s) 59, 211
MhlI GDGCHC 2 cut(s) 232, 774
MluCI AATT 7 cut(s) 192, 271, 304, 523, 755, 782, 856
MluI ACGCGT 1 cut(s) 794
MlyI GAGTC 1 cut(s) 623
MnlI CCTC 4 cut(s) 80, 83, 429, 832
MseI TTAA 2 cut(s) 41, 859
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 2 cut(s) 284, 662
MspR9I CCNGG 1 cut(s) 301
MvaI CCWGG 1 cut(s) 301
MvnI CGCG 1 cut(s) 796
MwoI GCNNNNNNNGC 2 cut(s) 154, 195
NdeII GATC 3 cut(s) 313, 802, 850
NlaIII CATG 3 cut(s) 55, 639, 675
NlaIV GGNNCC 2 cut(s) 17, 115
NspI RCATGY 1 cut(s) 675
PaqCI CACCTGC 1 cut(s) 838
PciI ACATGT 1 cut(s) 671
PcsI WCGNNNNNNNCGW 1 cut(s) 807
PfeI GAWTC 3 cut(s) 96, 296, 615
PfoI TCCNGGA 1 cut(s) 299
PkrI GCNGC 4 cut(s) 208, 254, 390, 641
PleI GAGTC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 623
Ppu21I YACGTR 1 cut(s) 238
PscI ACATGT 1 cut(s) 671
Psp124BI GAGCTC 2 cut(s) 232, 774
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 2 cut(s) 17, 115
PspPI GGNCC 2 cut(s) 15, 410
PstNI CAGNNNCTG 1 cut(s) 830
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 3 cut(s) 448, 690, 731
RsaNI GTAC 3 cut(s) 447, 689, 730
SacI GAGCTC 2 cut(s) 232, 774
SaqAI TTAA 2 cut(s) 41, 859
SatI GCNGC 4 cut(s) 207, 253, 389, 640
Sau3AI GATC 3 cut(s) 313, 802, 850
Sau96I GGNCC 2 cut(s) 15, 410
ScaI AGTACT 1 cut(s) 690
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 2 cut(s) 232, 774
SfaNI GCATC 4 cut(s) 270, 556, 692, 802
SfcI CTRYAG 3 cut(s) 289, 431, 553
SinI GGWCC 1 cut(s) 410
Sse9I AATT 7 cut(s) 192, 271, 304, 523, 755, 782, 856
SspMI CTAG 1 cut(s) 29
SstI GAGCTC 2 cut(s) 232, 774
StyD4I CCNGG 1 cut(s) 299
StyI CCWWGG 2 cut(s) 11, 223
TaaI ACNGT 1 cut(s) 693
TaiI ACGT 2 cut(s) 240, 452
TaqI TCGA 4 cut(s) 60, 153, 327, 801
TasI AATT 7 cut(s) 192, 271, 304, 523, 755, 782, 856
TatI WGTACW 1 cut(s) 688
TfiI GAWTC 3 cut(s) 96, 296, 615
Tru1I TTAA 2 cut(s) 41, 859
Tru9I TTAA 2 cut(s) 41, 859
TseI GCWGC 4 cut(s) 206, 252, 388, 639
TspDTI ATGAA 3 cut(s) 120, 253, 313
VpaK11BI GGWCC 1 cut(s) 410
XapI RAATTY 1 cut(s) 782
XbaI TCTAGA 1 cut(s) 28
XceI RCATGY 1 cut(s) 675
XmiI GTMKAC 1 cut(s) 465
XspI CTAG 1 cut(s) 29
ZrmI AGTACT 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.