MD14G1201200.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014174
Physical Location & Seq
Forward (+)
29027913 .. 29029634
1722 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1201200.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGAAGTTCACAACGTTGGCAGCAATGATGGTGGCCGTGGCCGTCTTCGGCCTCCTCATCGATGGCCACGACTGCAAAATCGTGCAGTTTATATTCGGGGACTCCCTCTCCGACGTTGGAAACAACAAGTACTTGAGCAAGAGTCTCGCCCAAGCAAGCCTGCCTTGGTATGGCATTGATATGGGCAATGGACTGCCTAATGGAAGGTTCTCTAATGGCCGTACCGTTGCTGATATAATAGGTGATACCATGGGGCTCCCACGGCCCCCGGCCTTTCTCGATCCGTCTTTAAACGAGGACATGATACTCGAAAACGGAGTGAACTACGCCTCTGGAGGTGGTGGGATTTTGAATGAAACCGGTGGCTACTTTATTCAGAGGCTTTCGCTCTACAAGCAGATCGAGCTTTTTCAAGGAACACAAACACTTATCAGAAGCAAAATCGGTGAACAAGCAGCGGAGAAGTTCTTCCAAGACGCTCGTTATGTGGTTGCTTTAGGCAGTAACGACTTCATTAACAACTACTTGATGCCTGTTTACAGTGATTCGTGGAGTTACAACGATCAAACTTTCATGGAATACTTAATGGGAACTCTAGAGAAGCAACTTAAGCTGTTGCACAGCCTAGGGGCGAGGCAGCTGATGGTGTTCGGGCTAGGACCAATGGGTTGTATTCCACTTCAAAGAGTGCTAAGTTCATCTTCGGCTTGCCAAGATAGAACAAACAAACTAGCTCTCAGCTTCAACAAAGCTGGTAGCAAGCTTATGGAAGATTTGTCCAGCCAGCTTCCTAACGCGAGCTATCGATTTGGTGACGCGTATGATGTTGTCAACAACGTGATCAGCGATCCAAACAAATACGGATTCAGCAATGCGGACTCACCATGCTGCTCATTTGGAAGAATTCGACCGGCTTTGACGTGCGTCCCTGCATCAGTACTGTGCAAAGACAGAAGCAAATATGTGTTTTGGGACGAGTACCATCCATCGGACAGTGCAAACGAGCTTATTGCCACGGAACTCATAAAGAAATTCGGTTTCTCGCGCGTTGGTGAAACAGATGGTCCTTCCCCTGCACCTGCCATTGCTCTATCACCAGAGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.2

Weight (kDa)

5.23

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 340 3e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74460
fragaria_vesca FvH4_5g14860
malus_domestica MD06G1194000.v1.1 MD14G1201200.v1.1
prunus_persica Prupe.5G194400_v2.0.a1
pyrus_communis pycom06g17190 pycom14g16670
rosa_chinensis RchiOBHm_Chr7g0181521
rosa_laevigata RLG00000005186
rosa_multiflora Rmu_sc0004621.1_g000032
rosa_roxburghii Rroxscaffold_3G00271930
rosa_rugosa Rorug06G0442000
rosa_samantha Rh7AG044100 Rh7BG043200 Rh7CG045000 Rh7DG043700
rosa_wichuraiana Rw7G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1087
Acc36I ACCTGC 1 cut(s) 1087
AccII CGCG 4 cut(s) 797, 818, 1045, 1047
AciI CCGC 2 cut(s) 458, 875
AclI AACGTT 1 cut(s) 14
AclWI GGATC 2 cut(s) 275, 842
AcoI YGGCCR 4 cut(s) 33, 39, 64, 217
AcsI RAATTY 2 cut(s) 903, 1031
AfaI GTAC 4 cut(s) 131, 223, 939, 980
AfiI CCNNNNNNNGG 2 cut(s) 170, 988
AflII CTTAAG 1 cut(s) 608
AflIII ACRYGT 1 cut(s) 816
AgeI ACCGGT 1 cut(s) 359
AgsI TTSAA 4 cut(s) 352, 413, 683, 745
AjiI CACGTC 1 cut(s) 921
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 2 cut(s) 275, 842
AoxI GGCC 7 cut(s) 33, 39, 49, 64, 217, 263, 270
ApeKI GCWGC 4 cut(s) 20, 455, 637, 888
ApoI RAATTY 2 cut(s) 903, 1031
AsiGI ACCGGT 1 cut(s) 359
Asp700I GAANNNNTTC 1 cut(s) 467
AspA2I CCTAGG 1 cut(s) 625
AspLEI GCGC 1 cut(s) 1047
AspS9I GGNCC 3 cut(s) 264, 659, 1064
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 6 cut(s) 254, 458, 824, 873, 1064, 1086
AvaII GGWCC 2 cut(s) 659, 1064
AvrII CCTAGG 1 cut(s) 625
BalI TGGCCA 1 cut(s) 66
BanII GRGCYC 1 cut(s) 258
BbsI GAAGAC 1 cut(s) 37
BbvI GCAGC 4 cut(s) 32, 467, 649, 875
BccI CCATC 6 cut(s) 22, 56, 637, 990, 994, 1055
BceAI ACGGC 4 cut(s) 20, 26, 204, 278
BcgI CGANNNNNNTGC 4 cut(s) 127, 161, 992, 1026
BclI TGATCA 1 cut(s) 840
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 4 cut(s) 596, 626, 656, 731
BfrI CTTAAG 1 cut(s) 608
BfuAI ACCTGC 1 cut(s) 1087
BisI GCNGC 4 cut(s) 21, 456, 638, 889
BlnI CCTAGG 1 cut(s) 625
BlsI GCNGC 4 cut(s) 22, 457, 639, 890
BmcAI AGTACT 2 cut(s) 131, 939
Bme1390I CCNGG 1 cut(s) 269
Bme18I GGWCC 2 cut(s) 659, 1064
BmgBI CACGTC 1 cut(s) 921
BmgT120I GGNCC 3 cut(s) 264, 659, 1064
BmiI GGNNCC 2 cut(s) 257, 266
BmrFI CCNGG 1 cut(s) 269
BmsI GCATC 2 cut(s) 519, 941
BoxI GACNNNNGTC 1 cut(s) 923
BpiI GAAGAC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 269
Bsa29I ATCGAT 2 cut(s) 60, 805
BsaJI CCNNGG 7 cut(s) 36, 164, 249, 260, 267, 625, 1014
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 988
Bse118I RCCGGY 2 cut(s) 359, 910
Bse3DI GCAATG 4 cut(s) 30, 193, 877, 1083
BseCI ATCGAT 2 cut(s) 60, 805
BseDI CCNNGG 7 cut(s) 36, 164, 249, 260, 267, 625, 1014
BseGI GGATG 1 cut(s) 982
BseLI CCNNNNNNNGG 2 cut(s) 170, 988
BseMI GCAATG 4 cut(s) 30, 193, 877, 1083
BseMII CTCAG 1 cut(s) 751
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 4 cut(s) 32, 467, 649, 875
BsgI GTGCAG 2 cut(s) 104, 1059
Bsh1236I CGCG 4 cut(s) 797, 818, 1045, 1047
Bsh1285I CGRYCG 1 cut(s) 911
BshFI GGCC 7 cut(s) 35, 41, 51, 66, 219, 265, 272
BshTI ACCGGT 1 cut(s) 359
BshVI ATCGAT 2 cut(s) 60, 805
BsiEI CGRYCG 1 cut(s) 911
BsiSI CCGG 3 cut(s) 269, 360, 911
BslFI GGGAC 3 cut(s) 113, 911, 986
BslI CCNNNNNNNGG 2 cut(s) 170, 988
BsmAI GTCTC 1 cut(s) 149
BsmFI GGGAC 3 cut(s) 113, 911, 986
BsnI GGCC 7 cut(s) 35, 41, 51, 66, 219, 265, 272
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 5 cut(s) 280, 399, 562, 840, 847
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 2 cut(s) 458, 875
BspANI GGCC 7 cut(s) 35, 41, 51, 66, 219, 265, 272
BspCNI CTCAG 1 cut(s) 750
BspDI ATCGAT 2 cut(s) 60, 805
BspFNI CGCG 4 cut(s) 797, 818, 1045, 1047
BspLI GGNNCC 2 cut(s) 257, 266
BspMI ACCTGC 1 cut(s) 1087
BspPI GGATC 2 cut(s) 275, 842
BspTI CTTAAG 1 cut(s) 608
BsrDI GCAATG 4 cut(s) 30, 193, 877, 1083
BsrFI RCCGGY 2 cut(s) 359, 910
BssAI RCCGGY 2 cut(s) 359, 910
BssECI CCNNGG 7 cut(s) 36, 164, 249, 260, 267, 625, 1014
BssMI GATC 5 cut(s) 280, 399, 562, 840, 847
BssT1I CCWWGG 3 cut(s) 164, 249, 625
Bst4CI ACNGT 4 cut(s) 226, 542, 942, 995
BstAFI CTTAAG 1 cut(s) 608
BstC8I GCNNGC 6 cut(s) 157, 161, 709, 761, 785, 799
BstDEI CTNAG 2 cut(s) 692, 737
BstDSI CCRYGG 4 cut(s) 36, 249, 260, 1014
BstF5I GGATG 1 cut(s) 982
BstFNI CGCG 4 cut(s) 797, 818, 1045, 1047
BstHHI GCGC 1 cut(s) 1047
BstKTI GATC 5 cut(s) 283, 402, 565, 843, 850
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 5 cut(s) 280, 399, 562, 840, 847
BstMCI CGRYCG 1 cut(s) 911
BstMWI GCNNNNNNNGC 5 cut(s) 72, 262, 394, 403, 610
BstPAI GACNNNNGTC 1 cut(s) 923
BstSCI CCNGG 1 cut(s) 267
BstUI CGCG 4 cut(s) 797, 818, 1045, 1047
BstV1I GCAGC 4 cut(s) 32, 467, 649, 875
BstV2I GAAGAC 1 cut(s) 37
Bsu15I ATCGAT 2 cut(s) 60, 805
BsuRI GGCC 7 cut(s) 35, 41, 51, 66, 219, 265, 272
BsuTUI ATCGAT 2 cut(s) 60, 805
BtgI CCRYGG 4 cut(s) 36, 249, 260, 1014
BtrI CACGTC 1 cut(s) 921
BtsCI GGATG 1 cut(s) 982
BtsIMutI CAGTG 2 cut(s) 547, 1000
BveI ACCTGC 1 cut(s) 1087
Cac8I GCNNGC 6 cut(s) 157, 161, 709, 761, 785, 799
CfoI GCGC 1 cut(s) 1047
Cfr10I RCCGGY 2 cut(s) 359, 910
Cfr13I GGNCC 3 cut(s) 264, 659, 1064
ClaI ATCGAT 2 cut(s) 60, 805
CseI GACGC 3 cut(s) 485, 824, 913
Csp6I GTAC 4 cut(s) 130, 222, 938, 979
CspAI ACCGGT 1 cut(s) 359
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 4 cut(s) 250, 301, 574, 885
CviQI GTAC 4 cut(s) 130, 222, 938, 979
DdeI CTNAG 2 cut(s) 692, 737
DpnI GATC 5 cut(s) 282, 401, 564, 842, 849
DpnII GATC 5 cut(s) 280, 399, 562, 840, 847
DraI TTTAAA 1 cut(s) 291
EaeI YGGCCR 4 cut(s) 33, 39, 64, 217
Eco130I CCWWGG 3 cut(s) 164, 249, 625
Eco24I GRGCYC 1 cut(s) 258
Eco47I GGWCC 2 cut(s) 659, 1064
EcoRI GAATTC 1 cut(s) 903
EcoT14I CCWWGG 3 cut(s) 164, 249, 625
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 3 cut(s) 164, 249, 625
FaeI CATG 4 cut(s) 253, 304, 577, 888
FalI AAGNNNNNCTT 4 cut(s) 148, 180, 685, 717
FaqI GGGAC 3 cut(s) 113, 911, 986
FatI CATG 4 cut(s) 249, 300, 573, 884
FbaI TGATCA 1 cut(s) 840
Fnu4HI GCNGC 4 cut(s) 21, 456, 638, 889
FokI GGATG 1 cut(s) 969
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 4 cut(s) 21, 456, 638, 889
FspBI CTAG 4 cut(s) 596, 626, 656, 731
GlaI GCGC 1 cut(s) 1046
GluI GCNGC 4 cut(s) 21, 456, 638, 889
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 7 cut(s) 35, 41, 51, 66, 219, 265, 272
HapII CCGG 3 cut(s) 269, 360, 911
HgaI GACGC 3 cut(s) 485, 824, 913
HhaI GCGC 1 cut(s) 1047
Hin1II CATG 4 cut(s) 253, 304, 577, 888
Hin6I GCGC 1 cut(s) 1045
HinP1I GCGC 1 cut(s) 1045
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 5 cut(s) 101, 142, 545, 864, 878
HpaII CCGG 3 cut(s) 269, 360, 911
HphI GGTGA 6 cut(s) 254, 458, 824, 873, 1064, 1086
Hpy166II GTNNAC 5 cut(s) 9, 322, 449, 538, 832
Hpy188I TCNGA 4 cut(s) 112, 378, 434, 991
Hpy188III TCNNGA 3 cut(s) 278, 333, 596
Hpy8I GTNNAC 5 cut(s) 9, 322, 449, 538, 832
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 2 cut(s) 198, 1077
HpyCH4III ACNGT 4 cut(s) 226, 542, 942, 995
HpyCH4IV ACGT 4 cut(s) 14, 114, 837, 920
HpyCH4V TGCA 7 cut(s) 75, 85, 619, 932, 945, 998, 1076
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 262, 394, 403, 610
HpyF3I CTNAG 2 cut(s) 692, 737
HpySE526I ACGT 4 cut(s) 14, 114, 837, 920
Hsp92II CATG 4 cut(s) 253, 304, 577, 888
HspAI GCGC 1 cut(s) 1045
Ksp22I TGATCA 1 cut(s) 840
Kzo9I GATC 5 cut(s) 280, 399, 562, 840, 847
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 4 cut(s) 32, 467, 649, 875
LweI GCATC 2 cut(s) 519, 941
MaeI CTAG 4 cut(s) 596, 626, 656, 731
MaeII ACGT 4 cut(s) 14, 114, 837, 920
MaeIII GTNAC 3 cut(s) 503, 554, 812
MalI GATC 5 cut(s) 282, 401, 564, 842, 849
MboI GATC 5 cut(s) 280, 399, 562, 840, 847
MboII GAAGA 5 cut(s) 37, 460, 693, 782, 912
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 66
MluCI AATT 2 cut(s) 903, 1031
MluI ACGCGT 1 cut(s) 816
MluNI TGGCCA 1 cut(s) 66
MlyI GAGTC 3 cut(s) 95, 151, 872
MmeI TCCRAC 2 cut(s) 97, 135
MnlI CCTC 9 cut(s) 62, 65, 116, 289, 329, 340, 372, 627, 1093
Mox20I TGGCCA 1 cut(s) 66
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 1 cut(s) 66
MseI TTAA 4 cut(s) 290, 516, 584, 609
MslI CAYNNNNRTG 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 66
MspA1I CMGCKG 2 cut(s) 458, 640
MspCI CTTAAG 1 cut(s) 608
MspI CCGG 3 cut(s) 269, 360, 911
MspR9I CCNGG 1 cut(s) 269
MvnI CGCG 4 cut(s) 797, 818, 1045, 1047
MwoI GCNNNNNNNGC 5 cut(s) 72, 262, 394, 403, 610
NciI CCSGG 1 cut(s) 269
NcoI CCATGG 1 cut(s) 249
NdeII GATC 5 cut(s) 280, 399, 562, 840, 847
NlaIII CATG 4 cut(s) 253, 304, 577, 888
NlaIV GGNNCC 2 cut(s) 257, 266
NmuCI GTSAC 1 cut(s) 812
PaqCI CACCTGC 1 cut(s) 1087
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 843
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 2 cut(s) 545, 864
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 4 cut(s) 22, 457, 639, 890
PleI GAGTC 3 cut(s) 95, 150, 872
PpsI GAGTC 3 cut(s) 95, 150, 872
PshAI GACNNNNGTC 1 cut(s) 923
Psp1406I AACGTT 1 cut(s) 14
PspN4I GGNNCC 2 cut(s) 257, 266
PspPI GGNCC 3 cut(s) 264, 659, 1064
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 4 cut(s) 131, 223, 939, 980
RsaNI GTAC 4 cut(s) 130, 222, 938, 979
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 4 cut(s) 290, 516, 584, 609
SatI GCNGC 4 cut(s) 21, 456, 638, 889
Sau3AI GATC 5 cut(s) 280, 399, 562, 840, 847
Sau96I GGNCC 3 cut(s) 264, 659, 1064
ScaI AGTACT 2 cut(s) 131, 939
SchI GAGTC 3 cut(s) 95, 151, 872
ScrFI CCNGG 1 cut(s) 269
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 2 cut(s) 519, 941
SinI GGWCC 2 cut(s) 659, 1064
SmiMI CAYNNNNRTG 1 cut(s) 179
SmlI CTYRAG 2 cut(s) 133, 608
SmoI CTYRAG 2 cut(s) 133, 608
Sse9I AATT 2 cut(s) 903, 1031
SsiI CCGC 2 cut(s) 458, 875
SspMI CTAG 4 cut(s) 596, 626, 656, 731
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 3 cut(s) 164, 249, 625
TaaI ACNGT 4 cut(s) 226, 542, 942, 995
TaiI ACGT 4 cut(s) 17, 117, 840, 923
TaqI TCGA 6 cut(s) 60, 279, 309, 402, 805, 907
TasI AATT 2 cut(s) 903, 1031
TatI WGTACW 2 cut(s) 129, 937
TfiI GAWTC 2 cut(s) 545, 864
Tru1I TTAA 4 cut(s) 290, 516, 584, 609
Tru9I TTAA 4 cut(s) 290, 516, 584, 609
TscAI CASTG 2 cut(s) 547, 1000
TseFI GTSAC 1 cut(s) 812
TseI GCWGC 4 cut(s) 20, 455, 637, 888
Tsp45I GTSAC 1 cut(s) 812
TspDTI ATGAA 5 cut(s) 17, 369, 502, 562, 687
TspGWI ACGGA 4 cut(s) 273, 330, 876, 1031
TspRI CASTG 2 cut(s) 547, 1000
Vha464I CTTAAG 1 cut(s) 608
VpaK11BI GGWCC 2 cut(s) 659, 1064
XapI RAATTY 2 cut(s) 903, 1031
XbaI TCTAGA 1 cut(s) 595
XmaJI CCTAGG 1 cut(s) 625
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 4 cut(s) 596, 626, 656, 731
ZrmI AGTACT 2 cut(s) 131, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.