MD06G1194000.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014174
Physical Location & Seq
Forward (+)
32892889 .. 32894585
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1194000.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGAAGTTCACAGCTCTAGCAGCAATGATGGCGGCCGTGACTGTCTTAGGCCTCCTCATTGATGGCCACGACTGCAAAATCGTGCAGTTTATATTCGGGGACTCCCTCTCCGACGTTGGAAACAACAAGCGCTTGAGCAGGAGTCTCGCCCAAGCAAGCCTGCCTTGGTATGGCATTGATATGGGCAATGGACTGCCCAATGGCAGGTTCACTAATGGCCGTACAGTTGCTGATATAATAGGTGATAACATGGGGCTCCCAAGGCCGCCGGCGGTTCTTGATCCGTCATTGACCGAAGACATGATACTTGAAAAAGGAGTAAACTATGCCTCTGGAGGTGGAGGGATTTTGAATGAAACCGGCGGCTACTTTATTCAGAAGCTCTCGCTCTACAGGCAGATCGATCTGTTTCAAGGGACACAAACACTGATCAAAAGCAAAATTGGCGCAAACGCAGCGGAGAAATTCTTCCAAGACGCTCGTTATGTGGTCGCTTTAGGAAGCAACGACTTCATTAACAACTACTTGATGCCTCTTTACAGAGATTCGTGGAGTTACAACGATCAAACATTCATCGAATACCTAATGGGAACTCTAGAAAGACAACTTAAGCTGTTGCACAGCCTAGGGGCGAGGCAGCTGATAGTGTTTGGGCTAGGACCAATGGGTTGTATTCCGCTCCAAAGGGTGCTAAGTACCTCTAACGGTTGCCAAGAGAGGTCAAACAAACTAGCTCTCAGCTTCAACAAAGCTGGAAACAAGCTAATGGAAGACTTGTCCAGTAAGCTTCCTAACGCAACCTATCGATTTGGTGATGCTTATGATGTCGTCAACGATGTGATCACCAATCCAATCCAATACGGATTCACCAATGCGGATTCACCGTGCTGCTCATTTGGAATAATTCGACCGGCTTTGACTTGTGTCCCTGCATCAGTACTGTGCAGTGACAGAAGCAAATACTTCTTTTGGGATGAGTACCATCCATCAGACAGTGCAAACGAGCTTATTGCCAAGGAACTCATAAAGAAATTCGGTTTGTCGCGCGTTGATGAAACGGATGGCCCTTCTCCTGCACCTGCTTTTGCTCCATCACCAGAGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.23

Weight (kDa)

5.52

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 341 3.6e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74460
fragaria_vesca FvH4_5g14860
malus_domestica MD06G1194000.v1.1 MD14G1201200.v1.1
prunus_persica Prupe.5G194400_v2.0.a1
pyrus_communis pycom06g17190 pycom14g16670
rosa_chinensis RchiOBHm_Chr7g0181521
rosa_laevigata RLG00000005186
rosa_multiflora Rmu_sc0004621.1_g000032
rosa_roxburghii Rroxscaffold_3G00271930
rosa_rugosa Rorug06G0442000
rosa_samantha Rh7AG044100 Rh7BG043200 Rh7CG045000 Rh7DG043700
rosa_wichuraiana Rw7G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1087
Acc36I ACCTGC 2 cut(s) 195, 1087
AccBSI CCGCTC 1 cut(s) 679
AccII CGCG 2 cut(s) 1045, 1047
AciI CCGC 7 cut(s) 32, 266, 272, 363, 458, 677, 875
AclWI GGATC 1 cut(s) 275
AcoI YGGCCR 3 cut(s) 33, 64, 217
AcsI RAATTY 2 cut(s) 464, 1031
AfaI GTAC 4 cut(s) 223, 697, 939, 980
AfeI AGCGCT 1 cut(s) 131
AfiI CCNNNNNNNGG 2 cut(s) 170, 204
AflII CTTAAG 1 cut(s) 608
AgsI TTSAA 4 cut(s) 311, 352, 413, 745
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 1 cut(s) 275
AlwNI CAGNNNCTG 1 cut(s) 230
Aor51HI AGCGCT 1 cut(s) 131
AoxI GGCC 6 cut(s) 33, 49, 64, 217, 263, 1063
ApeKI GCWGC 4 cut(s) 20, 455, 637, 888
ApoI RAATTY 2 cut(s) 464, 1031
Asp700I GAANNNNTTC 1 cut(s) 467
AspA2I CCTAGG 1 cut(s) 625
AspLEI GCGC 3 cut(s) 132, 449, 1047
AspS9I GGNCC 2 cut(s) 659, 1064
AsuHPI GGTGA 6 cut(s) 254, 824, 835, 859, 873, 1086
AvaII GGWCC 1 cut(s) 659
AvrII CCTAGG 1 cut(s) 625
BalI TGGCCA 1 cut(s) 66
BanII GRGCYC 1 cut(s) 258
BarI GAAGNNNNNNTAC 2 cut(s) 288, 320
BbsI GAAGAC 2 cut(s) 303, 777
BbvI GCAGC 4 cut(s) 32, 467, 649, 875
BccI CCATC 6 cut(s) 22, 56, 990, 994, 1055, 1099
BceAI ACGGC 2 cut(s) 20, 204
BcgI CGANNNNNNTGC 4 cut(s) 127, 161, 992, 1026
BclI TGATCA 2 cut(s) 429, 840
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 5 cut(s) 17, 596, 626, 656, 731
BfmI CTRYAG 1 cut(s) 391
BfoI RGCGCY 1 cut(s) 133
BfrI CTTAAG 1 cut(s) 608
BfuAI ACCTGC 2 cut(s) 195, 1087
BisI GCNGC 7 cut(s) 21, 33, 266, 364, 456, 638, 889
BlnI CCTAGG 1 cut(s) 625
BlsI GCNGC 7 cut(s) 22, 34, 267, 365, 457, 639, 890
BmcAI AGTACT 1 cut(s) 939
Bme18I GGWCC 1 cut(s) 659
BmgT120I GGNCC 2 cut(s) 659, 1064
BmiI GGNNCC 1 cut(s) 257
BmsI GCATC 3 cut(s) 519, 805, 941
BoxI GACNNNNGTC 1 cut(s) 923
BpiI GAAGAC 2 cut(s) 303, 777
BpmI CTGGAG 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 154
Bsa29I ATCGAT 2 cut(s) 402, 805
BsaJI CCNNGG 4 cut(s) 164, 260, 625, 1014
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 204
Bse118I RCCGGY 3 cut(s) 268, 359, 910
Bse1I ACTGG 1 cut(s) 780
Bse3DI GCAATG 2 cut(s) 30, 193
BseCI ATCGAT 2 cut(s) 402, 805
BseDI CCNNGG 4 cut(s) 164, 260, 625, 1014
BseGI GGATG 3 cut(s) 979, 982, 1066
BseLI CCNNNNNNNGG 2 cut(s) 170, 204
BseMI GCAATG 2 cut(s) 30, 193
BseMII CTCAG 1 cut(s) 751
BseNI ACTGG 1 cut(s) 780
BseRI GAGGAG 1 cut(s) 44
BseX3I CGGCCG 1 cut(s) 33
BseXI GCAGC 4 cut(s) 32, 467, 649, 875
BsgI GTGCAG 3 cut(s) 104, 964, 1059
Bsh1236I CGCG 2 cut(s) 1045, 1047
Bsh1285I CGRYCG 2 cut(s) 36, 911
BshFI GGCC 6 cut(s) 35, 51, 66, 219, 265, 1065
BshVI ATCGAT 2 cut(s) 402, 805
BsiEI CGRYCG 2 cut(s) 36, 911
BsiSI CCGG 3 cut(s) 269, 360, 911
BslFI GGGAC 3 cut(s) 113, 430, 911
BslI CCNNNNNNNGG 2 cut(s) 170, 204
BsmAI GTCTC 1 cut(s) 149
BsmFI GGGAC 3 cut(s) 113, 430, 911
BsnI GGCC 6 cut(s) 35, 51, 66, 219, 265, 1065
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 6 cut(s) 280, 399, 403, 429, 562, 840
BspACI CCGC 7 cut(s) 32, 266, 272, 363, 458, 677, 875
BspANI GGCC 6 cut(s) 35, 51, 66, 219, 265, 1065
BspCNI CTCAG 1 cut(s) 750
BspDI ATCGAT 2 cut(s) 402, 805
BspFNI CGCG 2 cut(s) 1045, 1047
BspLI GGNNCC 1 cut(s) 257
BspMI ACCTGC 2 cut(s) 195, 1087
BspPI GGATC 1 cut(s) 275
BspTI CTTAAG 1 cut(s) 608
BsrBI CCGCTC 1 cut(s) 679
BsrDI GCAATG 2 cut(s) 30, 193
BsrFI RCCGGY 3 cut(s) 268, 359, 910
BsrI ACTGG 1 cut(s) 780
BssAI RCCGGY 3 cut(s) 268, 359, 910
BssECI CCNNGG 4 cut(s) 164, 260, 625, 1014
BssMI GATC 6 cut(s) 280, 399, 403, 429, 562, 840
BssT1I CCWWGG 4 cut(s) 164, 260, 625, 1014
Bst4CI ACNGT 6 cut(s) 43, 226, 707, 885, 942, 995
BstAFI CTTAAG 1 cut(s) 608
BstC8I GCNNGC 3 cut(s) 157, 161, 270
BstDEI CTNAG 3 cut(s) 46, 692, 737
BstF5I GGATG 3 cut(s) 979, 982, 1066
BstFNI CGCG 2 cut(s) 1045, 1047
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 3 cut(s) 132, 449, 1047
BstKTI GATC 6 cut(s) 283, 402, 406, 432, 565, 843
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 6 cut(s) 280, 399, 403, 429, 562, 840
BstMCI CGRYCG 2 cut(s) 36, 911
BstMWI GCNNNNNNNGC 7 cut(s) 20, 29, 72, 262, 394, 444, 455
BstPAI GACNNNNGTC 1 cut(s) 923
BstSFI CTRYAG 1 cut(s) 391
BstUI CGCG 2 cut(s) 1045, 1047
BstV1I GCAGC 4 cut(s) 32, 467, 649, 875
BstV2I GAAGAC 2 cut(s) 303, 777
BstZI CGGCCG 1 cut(s) 33
Bsu15I ATCGAT 2 cut(s) 402, 805
BsuRI GGCC 6 cut(s) 35, 51, 66, 219, 265, 1065
BsuTUI ATCGAT 2 cut(s) 402, 805
BtsCI GGATG 3 cut(s) 979, 982, 1066
BtsI GCAGTG 1 cut(s) 952
BtsIMutI CAGTG 3 cut(s) 425, 952, 1000
BveI ACCTGC 2 cut(s) 195, 1087
Cac8I GCNNGC 3 cut(s) 157, 161, 270
CaiI CAGNNNCTG 1 cut(s) 230
CfoI GCGC 3 cut(s) 132, 449, 1047
Cfr10I RCCGGY 3 cut(s) 268, 359, 910
Cfr13I GGNCC 2 cut(s) 659, 1064
ClaI ATCGAT 2 cut(s) 402, 805
CseI GACGC 1 cut(s) 485
Csp6I GTAC 4 cut(s) 222, 696, 938, 979
CviAII CATG 2 cut(s) 250, 301
CviQI GTAC 4 cut(s) 222, 696, 938, 979
DdeI CTNAG 3 cut(s) 46, 692, 737
DpnI GATC 6 cut(s) 282, 401, 405, 431, 564, 842
DpnII GATC 6 cut(s) 280, 399, 403, 429, 562, 840
EaeI YGGCCR 3 cut(s) 33, 64, 217
EagI CGGCCG 1 cut(s) 33
EclXI CGGCCG 1 cut(s) 33
Eco130I CCWWGG 4 cut(s) 164, 260, 625, 1014
Eco147I AGGCCT 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 659
Eco47III AGCGCT 1 cut(s) 131
Eco52I CGGCCG 1 cut(s) 33
EcoT14I CCWWGG 4 cut(s) 164, 260, 625, 1014
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 4 cut(s) 164, 260, 625, 1014
FaeI CATG 2 cut(s) 253, 304
FalI AAGNNNNNCTT 2 cut(s) 148, 180
FaqI GGGAC 3 cut(s) 113, 430, 911
FatI CATG 2 cut(s) 249, 300
FbaI TGATCA 2 cut(s) 429, 840
Fnu4HI GCNGC 7 cut(s) 21, 33, 266, 364, 456, 638, 889
FokI GGATG 3 cut(s) 969, 986, 1073
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 7 cut(s) 21, 33, 266, 364, 456, 638, 889
FspBI CTAG 5 cut(s) 17, 596, 626, 656, 731
GlaI GCGC 3 cut(s) 131, 448, 1046
GluI GCNGC 7 cut(s) 21, 33, 266, 364, 456, 638, 889
GsuI CTGGAG 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 6 cut(s) 35, 51, 66, 219, 265, 1065
HapII CCGG 3 cut(s) 269, 360, 911
HgaI GACGC 1 cut(s) 485
HhaI GCGC 3 cut(s) 132, 449, 1047
Hin1II CATG 2 cut(s) 253, 304
Hin6I GCGC 3 cut(s) 130, 447, 1045
HinP1I GCGC 3 cut(s) 130, 447, 1045
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 5 cut(s) 101, 142, 545, 864, 878
HpaII CCGG 3 cut(s) 269, 360, 911
HphI GGTGA 6 cut(s) 254, 824, 835, 859, 873, 1086
Hpy166II GTNNAC 4 cut(s) 9, 210, 322, 832
Hpy188I TCNGA 3 cut(s) 112, 378, 991
Hpy188III TCNNGA 3 cut(s) 278, 333, 596
Hpy8I GTNNAC 4 cut(s) 9, 210, 322, 832
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 1 cut(s) 1077
HpyCH4III ACNGT 6 cut(s) 43, 226, 707, 885, 942, 995
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 7 cut(s) 75, 85, 619, 932, 945, 998, 1076
HpyF10VI GCNNNNNNNGC 7 cut(s) 20, 29, 72, 262, 394, 444, 455
HpyF3I CTNAG 3 cut(s) 46, 692, 737
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 2 cut(s) 253, 304
HspAI GCGC 3 cut(s) 130, 447, 1045
KroI GCCGGC 1 cut(s) 268
KroNI GCCGGC 1 cut(s) 270
Ksp22I TGATCA 2 cut(s) 429, 840
Kzo9I GATC 6 cut(s) 280, 399, 403, 429, 562, 840
LmnI GCTCC 3 cut(s) 261, 684, 1093
Lsp1109I GCAGC 4 cut(s) 32, 467, 649, 875
LweI GCATC 3 cut(s) 519, 805, 941
MaeI CTAG 5 cut(s) 17, 596, 626, 656, 731
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 3 cut(s) 37, 554, 947
MalI GATC 6 cut(s) 282, 401, 405, 431, 564, 842
MbiI CCGCTC 1 cut(s) 679
MboI GATC 6 cut(s) 280, 399, 403, 429, 562, 840
MboII GAAGA 3 cut(s) 308, 460, 782
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 66
MluCI AATT 4 cut(s) 441, 464, 903, 1031
MluNI TGGCCA 1 cut(s) 66
MlyI GAGTC 2 cut(s) 95, 151
MmeI TCCRAC 2 cut(s) 97, 135
Mox20I TGGCCA 1 cut(s) 66
MreI CGCCGGCG 1 cut(s) 268
MroNI GCCGGC 1 cut(s) 268
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 1 cut(s) 66
MseI TTAA 2 cut(s) 516, 609
MslI CAYNNNNRTG 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 66
MspA1I CMGCKG 2 cut(s) 458, 640
MspCI CTTAAG 1 cut(s) 608
MspI CCGG 3 cut(s) 269, 360, 911
MvnI CGCG 2 cut(s) 1045, 1047
MwoI GCNNNNNNNGC 7 cut(s) 20, 29, 72, 262, 394, 444, 455
NaeI GCCGGC 1 cut(s) 270
NdeII GATC 6 cut(s) 280, 399, 403, 429, 562, 840
NgoMIV GCCGGC 1 cut(s) 268
NlaIII CATG 2 cut(s) 253, 304
NlaIV GGNNCC 1 cut(s) 257
NmuCI GTSAC 2 cut(s) 37, 947
PaqCI CACCTGC 1 cut(s) 1087
PceI AGGCCT 1 cut(s) 51
PdiI GCCGGC 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 3 cut(s) 545, 864, 878
PkrI GCNGC 7 cut(s) 22, 34, 267, 365, 457, 639, 890
PleI GAGTC 2 cut(s) 95, 150
PpsI GAGTC 2 cut(s) 95, 150
PshAI GACNNNNGTC 1 cut(s) 923
PspN4I GGNNCC 1 cut(s) 257
PspPI GGNCC 2 cut(s) 659, 1064
PstNI CAGNNNCTG 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 4 cut(s) 223, 697, 939, 980
RsaNI GTAC 4 cut(s) 222, 696, 938, 979
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 2 cut(s) 516, 609
SatI GCNGC 7 cut(s) 21, 33, 266, 364, 456, 638, 889
Sau3AI GATC 6 cut(s) 280, 399, 403, 429, 562, 840
Sau96I GGNCC 2 cut(s) 659, 1064
ScaI AGTACT 1 cut(s) 939
SchI GAGTC 2 cut(s) 95, 151
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 3 cut(s) 519, 805, 941
SfcI CTRYAG 1 cut(s) 391
SgrAI CRCCGGYG 1 cut(s) 268
SinI GGWCC 1 cut(s) 659
SmiMI CAYNNNNRTG 1 cut(s) 179
SmlI CTYRAG 2 cut(s) 133, 608
SmoI CTYRAG 2 cut(s) 133, 608
Sse9I AATT 4 cut(s) 441, 464, 903, 1031
SseBI AGGCCT 1 cut(s) 51
SsiI CCGC 7 cut(s) 32, 266, 272, 363, 458, 677, 875
SspMI CTAG 5 cut(s) 17, 596, 626, 656, 731
StuI AGGCCT 1 cut(s) 51
StyI CCWWGG 4 cut(s) 164, 260, 625, 1014
TaaI ACNGT 6 cut(s) 43, 226, 707, 885, 942, 995
TaiI ACGT 1 cut(s) 117
TaqI TCGA 4 cut(s) 402, 576, 805, 907
TaqII GACCGA 1 cut(s) 308
TasI AATT 4 cut(s) 441, 464, 903, 1031
TatI WGTACW 1 cut(s) 937
TauI GCSGC 3 cut(s) 35, 268, 366
TfiI GAWTC 3 cut(s) 545, 864, 878
Tru1I TTAA 2 cut(s) 516, 609
Tru9I TTAA 2 cut(s) 516, 609
TscAI CASTG 3 cut(s) 432, 952, 1000
TseFI GTSAC 2 cut(s) 37, 947
TseI GCWGC 4 cut(s) 20, 455, 637, 888
Tsp45I GTSAC 2 cut(s) 37, 947
TspDTI ATGAA 5 cut(s) 17, 369, 502, 562, 1068
TspGWI ACGGA 3 cut(s) 273, 876, 1073
TspRI CASTG 3 cut(s) 432, 952, 1000
Vha464I CTTAAG 1 cut(s) 608
VpaK11BI GGWCC 1 cut(s) 659
XapI RAATTY 2 cut(s) 464, 1031
XbaI TCTAGA 1 cut(s) 595
XmaJI CCTAGG 1 cut(s) 625
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 5 cut(s) 17, 596, 626, 656, 731
ZrmI AGTACT 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.