RLG00000005186

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014174
Physical Location & Seq
Reverse (-)
65717924 .. 65719475
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005186

Sequence Viewer

Length: 1107 bp
ATGAAGATCAAGGCGGGAGTAGCAATGTTGGTTGTGACCATCTTTACCATCCTAATTGATGGTTACAGCTGCAAAGTGGTGCAGTTTATATTTGGCGACTCTCTCTCGGATGTTGGAAACAACAAGTACTTGAGCAAGAGCCTCGCCCAAGCAAGCTTGCCTTGGTATGGTATCGATTTTGGTAATGGATTGCCTAATGGGAGGTTCTCTAATGGTCGTACAGTTGCTGATATTATAGGTGATAACATGGGTCTCCCAAGGCCCCCTGCCTATCTAGACCCATCTTTAACCGAAGACATGATACTCGAAAATGGAGTGAACTATGCTTCTGGAGGAGGTGGAATCTTGAATGAAACTGGTGCCTTATTTATTCAAAGGCTTTCACTCTACAAGCAAATCGAGCTTTTTGAAGGGACACAAACATTGATAAGGAGCAAAATTGGCAAAGTGGCAGCAGACAAGTTCTTCCAAGGAGCTCGTTACGTGGTTGCTTTAGGCAGCAATGACTTCATCAACAATTACTTGATGCCGGTCTATAGCGATTCCTGGAATTACAACGATCAAAGCTTCATCGACTACTTGATGGAAACACTAAAGGCACAACTTCAGCTATTGCATACTTTAGGGGCAAGGCAGCTGATGGTGTTTGGTTTAGGACCAATGGGTTGTATTCCACTACAGAGGGTTCTAAGTACGTCTGGGGATTGCGTAGACAGAGCAAACAACTTAGCTCTCAGCTTCAACAAAGCTGGAAGCAAGCTAATGGACGATTTGAACGCCAAACTTCCCAACTCCAGCTATAGATTTGGTGATGCTTATGATGTGGTCAATAATATCATCATCAATCCAACCAGATATGGATTCAATAACTCAGACTCACCATGCTGCTCATTCGGAAAAATAAGACCGGCTCTAACATGTGTGCCTGCATCAGTACTGTGCAAAGACAGAAGCAAATATCTGTTCTGGGATGAGTACCACCCATCGGATAGCGCTAACCAATTTATTGCTAATGAGCTCATCAAGAAATTCGGTTTCACGCGTGTCGATCAAGGCAATGCTCCTTCCTCAGCACCTGCCATTGCTCCATCACCGGATCAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.3

Weight (kDa)

6.16

Isoelectric Point (pI)

29.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 340 1.2e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74460
fragaria_vesca FvH4_5g14860
malus_domestica MD06G1194000.v1.1 MD14G1201200.v1.1
prunus_persica Prupe.5G194400_v2.0.a1
pyrus_communis pycom06g17190 pycom14g16670
rosa_chinensis RchiOBHm_Chr7g0181521
rosa_laevigata RLG00000005186
rosa_multiflora Rmu_sc0004621.1_g000032
rosa_roxburghii Rroxscaffold_3G00271930
rosa_rugosa Rorug06G0442000
rosa_samantha Rh7AG044100 Rh7BG043200 Rh7CG045000 Rh7DG043700
rosa_wichuraiana Rw7G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1084
Acc36I ACCTGC 1 cut(s) 1084
AccB1I GGYRCC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 711
AccII CGCG 1 cut(s) 1042
AciI CCGC 1 cut(s) 14
AclWI GGATC 1 cut(s) 1104
AcsI RAATTY 1 cut(s) 1028
AcuI CTGAAG 1 cut(s) 590
AfaI GTAC 5 cut(s) 128, 220, 694, 936, 977
AfeI AGCGCT 1 cut(s) 994
AfiI CCNNNNNNNGG 2 cut(s) 167, 985
AflIII ACRYGT 2 cut(s) 917, 1040
AgsI TTSAA 6 cut(s) 349, 374, 410, 742, 775, 865
AjnI CCWGG 1 cut(s) 545
Alw21I GWGCWC 2 cut(s) 478, 1020
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 1 cut(s) 1104
AlwNI CAGNNNCTG 2 cut(s) 227, 1076
Aor51HI AGCGCT 1 cut(s) 994
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 5 cut(s) 69, 452, 498, 634, 885
ApoI RAATTY 1 cut(s) 1028
AspLEI GCGC 1 cut(s) 995
AspS9I GGNCC 2 cut(s) 261, 656
AsuHPI GGTGA 4 cut(s) 251, 821, 870, 1083
AvaII GGWCC 1 cut(s) 656
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 2 cut(s) 478, 1020
BarI GAAGNNNNNNTAC 2 cut(s) 285, 317
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 2 cut(s) 478, 1020
BbvCI CCTCAGC 1 cut(s) 1069
BbvI GCAGC 5 cut(s) 56, 464, 510, 646, 872
BccI CCATC 8 cut(s) 47, 53, 56, 289, 577, 634, 991, 1096
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 1 cut(s) 547
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 3 cut(s) 535, 677, 799
BfoI RGCGCY 1 cut(s) 996
BfuAI ACCTGC 1 cut(s) 1084
BisI GCNGC 5 cut(s) 70, 453, 499, 635, 886
BlsI GCNGC 5 cut(s) 71, 454, 500, 636, 887
BmcAI AGTACT 2 cut(s) 128, 936
Bme1390I CCNGG 1 cut(s) 547
Bme18I GGWCC 1 cut(s) 656
BmgT120I GGNCC 2 cut(s) 261, 656
BmiI GGNNCC 2 cut(s) 263, 361
BmrFI CCNGG 1 cut(s) 547
BmsI GCATC 3 cut(s) 516, 802, 938
BpiI GAAGAC 1 cut(s) 300
BpmI CTGGAG 2 cut(s) 351, 778
Bpu10I CCTNAGC 1 cut(s) 1069
BpuEI CTTGAG 1 cut(s) 151
Bsa29I ATCGAT 1 cut(s) 174
BsaAI YACGTR 1 cut(s) 484
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 161, 257, 469
BsaWI WCCGGW 1 cut(s) 1093
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 985
Bse118I RCCGGY 2 cut(s) 529, 907
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 4 cut(s) 30, 508, 1063, 1080
BseBI CCWGG 1 cut(s) 547
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 3 cut(s) 161, 257, 469
BseGI GGATG 3 cut(s) 48, 115, 976
BseLI CCNNNNNNNGG 2 cut(s) 167, 985
BseMI GCAATG 4 cut(s) 30, 508, 1063, 1080
BseMII CTCAG 3 cut(s) 748, 885, 1083
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 1 cut(s) 348
BseXI GCAGC 5 cut(s) 56, 464, 510, 646, 872
BsgI GTGCAG 1 cut(s) 101
Bsh1236I CGCG 1 cut(s) 1042
BshFI GGCC 1 cut(s) 262
BshNI GGYRCC 1 cut(s) 359
BshVI ATCGAT 1 cut(s) 174
BsiHKAI GWGCWC 2 cut(s) 478, 1020
BsiSI CCGG 3 cut(s) 530, 908, 1094
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 2 cut(s) 167, 985
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 427
BsnI GGCC 1 cut(s) 262
Bso31I GGTCTC 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 478, 1020
Bsp143I GATC 4 cut(s) 6, 559, 1048, 1096
BspACI CCGC 1 cut(s) 14
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 3 cut(s) 747, 884, 1082
BspDI ATCGAT 1 cut(s) 174
BspFNI CGCG 1 cut(s) 1042
BspLI GGNNCC 2 cut(s) 263, 361
BspMI ACCTGC 1 cut(s) 1084
BspPI GGATC 1 cut(s) 1104
BspT107I GGYRCC 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 4 cut(s) 30, 508, 1063, 1080
BsrFI RCCGGY 2 cut(s) 529, 907
BsrI ACTGG 1 cut(s) 361
BssAI RCCGGY 2 cut(s) 529, 907
BssECI CCNNGG 3 cut(s) 161, 257, 469
BssMI GATC 4 cut(s) 6, 559, 1048, 1096
BssT1I CCWWGG 3 cut(s) 161, 257, 469
Bst2UI CCWGG 1 cut(s) 547
Bst4CI ACNGT 2 cut(s) 223, 939
BstBAI YACGTR 1 cut(s) 484
BstC8I GCNNGC 4 cut(s) 154, 158, 758, 927
BstDEI CTNAG 5 cut(s) 689, 727, 734, 871, 1069
BstF5I GGATG 3 cut(s) 48, 115, 976
BstFNI CGCG 1 cut(s) 1042
BstH2I RGCGCY 1 cut(s) 996
BstHHI GCGC 1 cut(s) 995
BstKTI GATC 4 cut(s) 9, 562, 1051, 1099
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 4 cut(s) 6, 559, 1048, 1096
BstMWI GCNNNNNNNGC 3 cut(s) 20, 400, 441
BstNI CCWGG 1 cut(s) 547
BstNSI RCATGY 1 cut(s) 921
BstSCI CCNGG 1 cut(s) 545
BstSFI CTRYAG 3 cut(s) 535, 677, 799
BstUI CGCG 1 cut(s) 1042
BstV1I GCAGC 5 cut(s) 56, 464, 510, 646, 872
BstV2I GAAGAC 1 cut(s) 300
Bsu15I ATCGAT 1 cut(s) 174
BsuRI GGCC 1 cut(s) 262
BsuTUI ATCGAT 1 cut(s) 174
BtsCI GGATG 3 cut(s) 48, 115, 976
BveI ACCTGC 1 cut(s) 1084
Cac8I GCNNGC 4 cut(s) 154, 158, 758, 927
CaiI CAGNNNCTG 2 cut(s) 227, 1076
CfoI GCGC 1 cut(s) 995
Cfr10I RCCGGY 2 cut(s) 529, 907
Cfr13I GGNCC 2 cut(s) 261, 656
ClaI ATCGAT 1 cut(s) 174
Csp6I GTAC 5 cut(s) 127, 219, 693, 935, 976
CviAII CATG 4 cut(s) 247, 298, 882, 918
CviQI GTAC 5 cut(s) 127, 219, 693, 935, 976
DdeI CTNAG 5 cut(s) 689, 727, 734, 871, 1069
DpnI GATC 4 cut(s) 8, 561, 1050, 1098
DpnII GATC 4 cut(s) 6, 559, 1048, 1096
Ecl136II GAGCTC 2 cut(s) 476, 1018
Eco130I CCWWGG 3 cut(s) 161, 257, 469
Eco24I GRGCYC 2 cut(s) 478, 1020
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 656
Eco47III AGCGCT 1 cut(s) 994
Eco53kI GAGCTC 2 cut(s) 476, 1018
Eco57I CTGAAG 1 cut(s) 590
EcoICRI GAGCTC 2 cut(s) 476, 1018
EcoO109I RGGNCCY 1 cut(s) 261
EcoRII CCWGG 1 cut(s) 545
EcoT14I CCWWGG 3 cut(s) 161, 257, 469
EcoT38I GRGCYC 2 cut(s) 478, 1020
ErhI CCWWGG 3 cut(s) 161, 257, 469
FaeI CATG 4 cut(s) 250, 301, 885, 921
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FaqI GGGAC 1 cut(s) 427
FatI CATG 4 cut(s) 246, 297, 881, 917
FauI CCCGC 1 cut(s) 7
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 5 cut(s) 70, 453, 499, 635, 886
FokI GGATG 3 cut(s) 35, 122, 983
FriOI GRGCYC 2 cut(s) 478, 1020
Fsp4HI GCNGC 5 cut(s) 70, 453, 499, 635, 886
FspBI CTAG 1 cut(s) 275
GlaI GCGC 1 cut(s) 994
GluI GCNGC 5 cut(s) 70, 453, 499, 635, 886
GsuI CTGGAG 2 cut(s) 351, 778
HaeII RGCGCY 1 cut(s) 996
HaeIII GGCC 1 cut(s) 262
HapII CCGG 3 cut(s) 530, 908, 1094
HhaI GCGC 1 cut(s) 995
Hin1II CATG 4 cut(s) 250, 301, 885, 921
Hin6I GCGC 1 cut(s) 993
HinP1I GCGC 1 cut(s) 993
HindIII AAGCTT 2 cut(s) 154, 565
HinfI GANTC 5 cut(s) 98, 342, 542, 861, 875
HpaII CCGG 3 cut(s) 530, 908, 1094
HphI GGTGA 4 cut(s) 251, 821, 870, 1083
Hpy166II GTNNAC 2 cut(s) 319, 712
Hpy188I TCNGA 5 cut(s) 109, 874, 896, 988, 1101
Hpy188III TCNNGA 4 cut(s) 275, 330, 346, 1024
Hpy8I GTNNAC 2 cut(s) 319, 712
HpyAV CCTTC 2 cut(s) 404, 1074
HpyCH4III ACNGT 2 cut(s) 223, 939
HpyCH4IV ACGT 2 cut(s) 483, 695
HpyCH4V TGCA 5 cut(s) 72, 82, 616, 929, 942
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 400, 441
HpyF3I CTNAG 5 cut(s) 689, 727, 734, 871, 1069
HpySE526I ACGT 2 cut(s) 483, 695
Hsp92II CATG 4 cut(s) 250, 301, 885, 921
HspAI GCGC 1 cut(s) 993
Kzo9I GATC 4 cut(s) 6, 559, 1048, 1096
LmnI GCTCC 4 cut(s) 432, 473, 1066, 1090
Lsp1109I GCAGC 5 cut(s) 56, 464, 510, 646, 872
LweI GCATC 3 cut(s) 516, 802, 938
MaeI CTAG 1 cut(s) 275
MaeII ACGT 2 cut(s) 483, 695
MaeIII GTNAC 3 cut(s) 34, 62, 479
MalI GATC 4 cut(s) 8, 561, 1050, 1098
MboI GATC 4 cut(s) 6, 559, 1048, 1096
MboII GAAGA 3 cut(s) 16, 305, 457
MhlI GDGCHC 2 cut(s) 478, 1020
MluCI AATT 7 cut(s) 54, 438, 517, 550, 1001, 1028, 1102
MluI ACGCGT 1 cut(s) 1040
MlyI GAGTC 2 cut(s) 92, 869
MmeI TCCRAC 2 cut(s) 94, 872
MnlI CCTC 6 cut(s) 152, 195, 326, 329, 675, 1078
MseI TTAA 2 cut(s) 287, 1105
MspA1I CMGCKG 2 cut(s) 69, 637
MspI CCGG 3 cut(s) 530, 908, 1094
MspR9I CCNGG 1 cut(s) 547
MvaI CCWGG 1 cut(s) 547
MvnI CGCG 1 cut(s) 1042
MwoI GCNNNNNNNGC 3 cut(s) 20, 400, 441
NdeII GATC 4 cut(s) 6, 559, 1048, 1096
NlaIII CATG 4 cut(s) 250, 301, 885, 921
NlaIV GGNNCC 2 cut(s) 263, 361
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 921
PaqCI CACCTGC 1 cut(s) 1084
PciI ACATGT 1 cut(s) 917
PfeI GAWTC 3 cut(s) 342, 542, 861
PfoI TCCNGGA 1 cut(s) 545
PkrI GCNGC 5 cut(s) 71, 454, 500, 636, 887
PleI GAGTC 2 cut(s) 92, 869
PpsI GAGTC 2 cut(s) 92, 869
Ppu21I YACGTR 1 cut(s) 484
PscI ACATGT 1 cut(s) 917
Psp124BI GAGCTC 2 cut(s) 478, 1020
Psp6I CCWGG 1 cut(s) 545
PspGI CCWGG 1 cut(s) 545
PspN4I GGNNCC 2 cut(s) 263, 361
PspPI GGNCC 2 cut(s) 261, 656
PstNI CAGNNNCTG 2 cut(s) 227, 1076
PvuII CAGCTG 2 cut(s) 69, 637
RsaI GTAC 5 cut(s) 128, 220, 694, 936, 977
RsaNI GTAC 5 cut(s) 127, 219, 693, 935, 976
SacI GAGCTC 2 cut(s) 478, 1020
SaqAI TTAA 2 cut(s) 287, 1105
SatI GCNGC 5 cut(s) 70, 453, 499, 635, 886
Sau3AI GATC 4 cut(s) 6, 559, 1048, 1096
Sau96I GGNCC 2 cut(s) 261, 656
ScaI AGTACT 2 cut(s) 128, 936
SchI GAGTC 2 cut(s) 92, 869
ScrFI CCNGG 1 cut(s) 547
SduI GDGCHC 2 cut(s) 478, 1020
SfaNI GCATC 3 cut(s) 516, 802, 938
SfcI CTRYAG 3 cut(s) 535, 677, 799
SinI GGWCC 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 130
SmoI CTYRAG 1 cut(s) 130
Sse9I AATT 7 cut(s) 54, 438, 517, 550, 1001, 1028, 1102
SsiI CCGC 1 cut(s) 14
SspMI CTAG 1 cut(s) 275
SstI GAGCTC 2 cut(s) 478, 1020
StyD4I CCNGG 1 cut(s) 545
StyI CCWWGG 3 cut(s) 161, 257, 469
TaaI ACNGT 2 cut(s) 223, 939
TaiI ACGT 2 cut(s) 486, 698
TaqI TCGA 5 cut(s) 174, 306, 399, 573, 1047
TasI AATT 7 cut(s) 54, 438, 517, 550, 1001, 1028, 1102
TatI WGTACW 2 cut(s) 126, 934
TfiI GAWTC 3 cut(s) 342, 542, 861
Tru1I TTAA 2 cut(s) 287, 1105
Tru9I TTAA 2 cut(s) 287, 1105
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 5 cut(s) 69, 452, 498, 634, 885
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 4 cut(s) 17, 366, 499, 559
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 1 cut(s) 1028
XbaI TCTAGA 1 cut(s) 274
XceI RCATGY 1 cut(s) 921
XmiI GTMKAC 1 cut(s) 711
XspI CTAG 1 cut(s) 275
ZrmI AGTACT 2 cut(s) 128, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.