AT1G75510

Transcription initiation factor IIF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0018284
Physical Location & Seq
Reverse (-)
28346998 .. 28348776
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G75510.1

Sequence Viewer

Length: 786 bp
ATGGAAGATATTCATAATCTCGATATAGAGAAAAGTGATAGATCGATTTGGTTGATGAAGTGTCCTGTAGTTGTAGACAAGGCATGGCACAAGATAGCAGCTTCGTCGTCTTCTTCTTTTGCTTCCTCGGATTCTCCTCCCGACATGGCAAAGATTGTTCGCGAAGTTGATCCTCTACGGGACGATTCTCCCCCCGAGTTCAAAATGTATATGGTTGGTGCTGAGTATGGGAACATGCCAAAGTGCTACGCTTTGAACATGTTCACAGACTTTGTTCCGATGGGGGGCTTTTCCGATGTTAATCAAGGTTGTGCAGCTGCTGAAGGAAAGGTTGATCACAAATTCGATATGAAGCCATATGGTGAAACCATTGAAGAATATGCTAGGCTTTGTCGTGAGAGAACGAGCAAAGCCATGGTTAAAAATCGACAGATTCAGGTTATTGATAATGACCGCGGAGTTCATATGAGGCCCATGCCTGGAATGCTTGGTTTAGTTTCCTCCAATTCCAAGGAAAAGAGGAAACCTCCCCCAGTCAAGCAAACCGAGGTGAAGAGGACGAGGAGAGATCGTGGTGAGCTTGAAGCTATAATGTTCAAGCTATTTGAAGGCCAGCCTAACTGGACATTGAAGCAGCTTGTACAAGAGACGGACCAGCCAGCTCAGTTCCTGAAAGAAATACTAAACGAGCTGTGCGTGTACAATAAGAGAGGATCGAACCAAGGGACATATGAGCTTAAACCTGAATACAAGAAATCTGCTGAAGATGATACAGGTGGGCAGTGA

Protein Analysis

261

Amino Acids

29.71

Weight (kDa)

6.47

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_beta PF02270 188 - 251 3.2e-19 TFIIF, beta subunit HTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018284)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75510
fragaria_vesca FvH4_2g38930
malus_domestica MD08G1022300.v1.1 MD15G1019500.v1.1 MD15G1019600.v1.1
prunus_persica Prupe.1G371700_v2.0.a1
pyrus_communis pycom08g01720 pycom15g01620 pycom15g01630
rosa_multiflora Rmu_sc0006892.1_g000007

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 75
AccII CGCG 2 cut(s) 162, 456
AciI CCGC 2 cut(s) 454, 456
AclWI GGATC 2 cut(s) 164, 721
AcsI RAATTY 1 cut(s) 341
AcuI CTGAAG 2 cut(s) 342, 783
AfaI GTAC 2 cut(s) 642, 701
AfiI CCNNNNNNNGG 2 cut(s) 284, 479
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 7 cut(s) 202, 256, 374, 584, 598, 608, 631
AjnI CCWGG 1 cut(s) 478
AluBI AGCT 9 cut(s) 101, 317, 580, 587, 601, 637, 662, 691, 736
AluI AGCT 9 cut(s) 101, 317, 580, 587, 601, 637, 662, 691, 736
Alw26I GTCTC 1 cut(s) 641
AlwI GGATC 2 cut(s) 164, 721
AlwNI CAGNNNCTG 2 cut(s) 320, 670
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 2 cut(s) 470, 610
ApeKI GCWGC 4 cut(s) 98, 314, 317, 634
ApoI RAATTY 1 cut(s) 341
Asp700I GAANNNNTTC 2 cut(s) 9, 260
AspS9I GGNCC 2 cut(s) 471, 652
AsuHPI GGTGA 3 cut(s) 374, 562, 587
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 1 cut(s) 652
BbsI GAAGAC 1 cut(s) 102
BbvI GCAGC 4 cut(s) 110, 304, 326, 646
BccI CCATC 1 cut(s) 274
BcgI CGANNNNNNTGC 2 cut(s) 87, 121
BciT130I CCWGG 1 cut(s) 480
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 641
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 1 cut(s) 66
BisI GCNGC 4 cut(s) 99, 315, 318, 635
BlsI GCNGC 4 cut(s) 100, 316, 319, 636
Bme1390I CCNGG 1 cut(s) 480
Bme18I GGWCC 1 cut(s) 652
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 2 cut(s) 471, 652
BmrFI CCNGG 1 cut(s) 480
BmrI ACTGGG 1 cut(s) 527
BmuI ACTGGG 1 cut(s) 527
BpiI GAAGAC 1 cut(s) 102
BplI GAGNNNNNCTC 2 cut(s) 511, 543
Bsa29I ATCGAT 1 cut(s) 44
BsaBI GATNNNNATC 1 cut(s) 300
BsaJI CCNNGG 6 cut(s) 126, 414, 454, 510, 546, 721
BsaXI ACNNNNNCTCC 2 cut(s) 556, 586
Bsc4I CCNNNNNNNGG 2 cut(s) 284, 479
Bse1I ACTGG 2 cut(s) 533, 626
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 1 cut(s) 480
BseCI ATCGAT 1 cut(s) 44
BseDI CCNNGG 6 cut(s) 126, 414, 454, 510, 546, 721
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 2 cut(s) 284, 479
BseMII CTCAG 2 cut(s) 213, 677
BseNI ACTGG 2 cut(s) 533, 626
BseRI GAGGAG 2 cut(s) 126, 577
BseXI GCAGC 4 cut(s) 110, 304, 326, 646
BsgI GTGCAG 1 cut(s) 333
Bsh1236I CGCG 2 cut(s) 162, 456
BshFI GGCC 2 cut(s) 472, 612
BshVI ATCGAT 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 194
BslFI GGGAC 2 cut(s) 194, 739
BslI CCNNNNNNNGG 2 cut(s) 284, 479
BsmAI GTCTC 1 cut(s) 641
BsmBI CGTCTC 1 cut(s) 641
BsmFI GGGAC 2 cut(s) 194, 739
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 2 cut(s) 472, 612
BsoBI CYCGRG 1 cut(s) 194
Bsp1407I TGTACA 2 cut(s) 640, 699
Bsp143I GATC 5 cut(s) 41, 169, 334, 568, 713
Bsp19I CCATGG 1 cut(s) 414
Bsp68I TCGCGA 1 cut(s) 162
BspACI CCGC 2 cut(s) 454, 456
BspANI GGCC 2 cut(s) 472, 612
BspCNI CTCAG 2 cut(s) 214, 676
BspDI ATCGAT 1 cut(s) 44
BspFNI CGCG 2 cut(s) 162, 456
BspPI GGATC 2 cut(s) 164, 721
BsrGI TGTACA 2 cut(s) 640, 699
BsrI ACTGG 2 cut(s) 533, 626
BssECI CCNNGG 6 cut(s) 126, 414, 454, 510, 546, 721
BssMI GATC 5 cut(s) 41, 169, 334, 568, 713
BssT1I CCWWGG 3 cut(s) 414, 510, 721
Bst2UI CCWGG 1 cut(s) 480
Bst6I CTCTTC 1 cut(s) 548
BstAUI TGTACA 2 cut(s) 640, 699
BstC8I GCNNGC 2 cut(s) 614, 660
BstDEI CTNAG 2 cut(s) 222, 663
BstDSI CCRYGG 2 cut(s) 414, 454
BstFNI CGCG 2 cut(s) 162, 456
BstKTI GATC 5 cut(s) 44, 172, 337, 571, 716
BstMAI GTCTC 1 cut(s) 641
BstMBI GATC 5 cut(s) 41, 169, 334, 568, 713
BstMWI GCNNNNNNNGC 1 cut(s) 484
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 2 cut(s) 238, 262
BstSCI CCNGG 1 cut(s) 478
BstSFI CTRYAG 1 cut(s) 66
BstUI CGCG 2 cut(s) 162, 456
BstV1I GCAGC 4 cut(s) 110, 304, 326, 646
BstV2I GAAGAC 1 cut(s) 102
Bsu15I ATCGAT 1 cut(s) 44
BsuRI GGCC 2 cut(s) 472, 612
BsuTUI ATCGAT 1 cut(s) 44
BtgI CCRYGG 2 cut(s) 414, 454
BtuMI TCGCGA 1 cut(s) 162
Cac8I GCNNGC 2 cut(s) 614, 660
CaiI CAGNNNCTG 2 cut(s) 320, 670
Cfr13I GGNCC 2 cut(s) 471, 652
Cfr42I CCGCGG 1 cut(s) 457
ClaI ATCGAT 1 cut(s) 44
Csp6I GTAC 2 cut(s) 641, 700
CviAII CATG 6 cut(s) 84, 145, 235, 259, 415, 475
CviQI GTAC 2 cut(s) 641, 700
DdeI CTNAG 2 cut(s) 222, 663
DpnI GATC 5 cut(s) 43, 171, 336, 570, 715
DpnII GATC 5 cut(s) 41, 169, 334, 568, 713
Eam1104I CTCTTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 548
Eco130I CCWWGG 3 cut(s) 414, 510, 721
Eco47I GGWCC 1 cut(s) 652
Eco57I CTGAAG 2 cut(s) 342, 783
Eco88I CYCGRG 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 3 cut(s) 414, 510, 721
ErhI CCWWGG 3 cut(s) 414, 510, 721
Esp3I CGTCTC 1 cut(s) 641
FaeI CATG 6 cut(s) 87, 148, 238, 262, 418, 478
FaqI GGGAC 2 cut(s) 194, 739
FatI CATG 6 cut(s) 83, 144, 234, 258, 414, 474
FauNDI CATATG 3 cut(s) 358, 465, 730
FbaI TGATCA 1 cut(s) 334
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 4 cut(s) 99, 315, 318, 635
Fsp4HI GCNGC 4 cut(s) 99, 315, 318, 635
FspBI CTAG 1 cut(s) 384
GluI GCNGC 4 cut(s) 99, 315, 318, 635
HaeIII GGCC 2 cut(s) 472, 612
Hin1II CATG 6 cut(s) 87, 148, 238, 262, 418, 478
HinfI GANTC 3 cut(s) 131, 185, 433
HphI GGTGA 3 cut(s) 374, 562, 587
Hpy166II GTNNAC 3 cut(s) 76, 264, 700
Hpy188I TCNGA 3 cut(s) 130, 279, 295
Hpy188III TCNNGA 5 cut(s) 20, 140, 161, 395, 670
Hpy8I GTNNAC 3 cut(s) 76, 264, 700
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 2 cut(s) 317, 602
HpyCH4V TGCA 1 cut(s) 314
HpyF10VI GCNNNNNNNGC 1 cut(s) 484
HpyF3I CTNAG 2 cut(s) 222, 663
Hsp92II CATG 6 cut(s) 87, 148, 238, 262, 418, 478
Ksp22I TGATCA 1 cut(s) 334
KspI CCGCGG 1 cut(s) 457
Kzo9I GATC 5 cut(s) 41, 169, 334, 568, 713
Lsp1109I GCAGC 4 cut(s) 110, 304, 326, 646
MaeI CTAG 1 cut(s) 384
MalI GATC 5 cut(s) 43, 171, 336, 570, 715
MboI GATC 5 cut(s) 41, 169, 334, 568, 713
MboII GAAGA 6 cut(s) 17, 102, 105, 386, 565, 776
MluCI AATT 2 cut(s) 341, 505
MroXI GAANNNNTTC 2 cut(s) 9, 260
MseI TTAA 3 cut(s) 300, 420, 738
MspA1I CMGCKG 2 cut(s) 317, 456
MspR9I CCNGG 1 cut(s) 480
Mva1269I GAATGC 1 cut(s) 489
MvaI CCWGG 1 cut(s) 480
MvnI CGCG 2 cut(s) 162, 456
MwoI GCNNNNNNNGC 1 cut(s) 484
NcoI CCATGG 1 cut(s) 414
NdeI CATATG 3 cut(s) 358, 465, 730
NdeII GATC 5 cut(s) 41, 169, 334, 568, 713
NlaIII CATG 6 cut(s) 87, 148, 238, 262, 418, 478
NruI TCGCGA 1 cut(s) 162
NspI RCATGY 2 cut(s) 238, 262
PciI ACATGT 1 cut(s) 258
PcsI WCGNNNNNNNCGW 1 cut(s) 693
PctI GAATGC 1 cut(s) 489
PdmI GAANNNNTTC 2 cut(s) 9, 260
PfeI GAWTC 3 cut(s) 131, 185, 433
PkrI GCNGC 4 cut(s) 100, 316, 319, 636
PscI ACATGT 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 478
PspGI CCWGG 1 cut(s) 478
PspPI GGNCC 2 cut(s) 471, 652
PstNI CAGNNNCTG 2 cut(s) 320, 670
PvuII CAGCTG 1 cut(s) 317
RruI TCGCGA 1 cut(s) 162
RsaI GTAC 2 cut(s) 642, 701
RsaNI GTAC 2 cut(s) 641, 700
SacII CCGCGG 1 cut(s) 457
SaqAI TTAA 3 cut(s) 300, 420, 738
SatI GCNGC 4 cut(s) 99, 315, 318, 635
Sau3AI GATC 5 cut(s) 41, 169, 334, 568, 713
Sau96I GGNCC 2 cut(s) 471, 652
ScrFI CCNGG 1 cut(s) 480
SfcI CTRYAG 1 cut(s) 66
Sfr303I CCGCGG 1 cut(s) 457
SgrBI CCGCGG 1 cut(s) 457
SinI GGWCC 1 cut(s) 652
Sse9I AATT 2 cut(s) 341, 505
SsiI CCGC 2 cut(s) 454, 456
SspMI CTAG 1 cut(s) 384
StyD4I CCNGG 1 cut(s) 478
StyI CCWWGG 3 cut(s) 414, 510, 721
TaqI TCGA 5 cut(s) 21, 44, 345, 427, 716
TasI AATT 2 cut(s) 341, 505
TatI WGTACW 2 cut(s) 640, 699
TfiI GAWTC 3 cut(s) 131, 185, 433
Tru1I TTAA 3 cut(s) 300, 420, 738
Tru9I TTAA 3 cut(s) 300, 420, 738
TseI GCWGC 4 cut(s) 98, 314, 317, 634
TspDTI ATGAA 3 cut(s) 71, 365, 452
TspGWI ACGGA 1 cut(s) 665
VpaK11BI GGWCC 1 cut(s) 652
XapI RAATTY 1 cut(s) 341
XceI RCATGY 2 cut(s) 238, 262
XmiI GTMKAC 1 cut(s) 75
XmnI GAANNNNTTC 2 cut(s) 9, 260
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.