Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0018284

Physical Location & Seq

Genomic Coordinates Forward (+)

1134886 .. 1135730

Gene Structure 845 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1019500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 351 bp

ATGGGCGTCGAGAAGAGAGAGAGAGATTCGACGGAGGAGGAGCGCGGGAACAGTAGCAGCAGCACCCGGAACTTGGACATAGGGAGGGCGGAGGCTCCGGTGTGGCTGATGAAGTGCCCGGTGGTCGTCGCCAAGTCGTGGAGCTCCCACAACCCCGCCGATCCCCACCCAATCGCCAAAGTCGTCCTCTCCTTGGATCCCCTACGCGCCGACGACCCCACTGCCATGGAGTTCAAGATGGAGATGCCTGACTCTGCAGCTGCAAATTTGCCGAAAAGTTATTCATTGAATATGTACAAGGACTTTGTTCCCATGTGCGTTTTCTCCGAAACAAATCAAGGTGAGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 map03022

Pfam Domains

Protein Families

TFIIF_beta

Protein Analysis

117

Amino Acids

12.89

Weight (kDa)

4.97

Isoelectric Point (pI)

47.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TFIIF_beta_N	PF17683	32 - 114	4e-08	TFIIF, beta subunit N-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0018284)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75510
fragaria_vesca	FvH4_2g38930
malus_domestica	MD08G1022300.v1.1 MD15G1019500.v1.1 MD15G1019600.v1.1
prunus_persica	Prupe.1G371700_v2.0.a1
pyrus_communis	pycom08g01720 pycom15g01620 pycom15g01630
rosa_multiflora	Rmu_sc0006892.1_g000007

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	138
AccII	CGCG	2 cut(s)	45, 207
AciI	CCGC	3 cut(s)	45, 89, 156
AclWI	GGATC	3 cut(s)	155, 191, 204
AcsI	RAATTY	1 cut(s)	265
AcyI	GRCGYC	1 cut(s)	6
AfaI	GTAC	1 cut(s)	296
AfiI	CCNNNNNNNGG	3 cut(s)	73, 138, 193
AgsI	TTSAA	2 cut(s)	235, 289
AluBI	AGCT	3 cut(s)	144, 260, 346
AluI	AGCT	3 cut(s)	144, 260, 346
Alw21I	GWGCWC	1 cut(s)	146
AlwI	GGATC	3 cut(s)	155, 191, 204
ApeKI	GCWGC	4 cut(s)	57, 60, 257, 260
ApoI	RAATTY	1 cut(s)	265
AspLEI	GCGC	2 cut(s)	45, 209
AsuC2I	CCSGG	2 cut(s)	67, 119
BaeGI	GKGCMC	1 cut(s)	119
BamHI	GGATCC	1 cut(s)	196
BanII	GRGCYC	1 cut(s)	146
Bbv12I	GWGCWC	1 cut(s)	146
BbvI	GCAGC	4 cut(s)	69, 72, 247, 269
BccI	CCATC	1 cut(s)	232
BcgI	CGANNNNNNTGC	2 cut(s)	203, 237
BcnI	CCSGG	2 cut(s)	67, 119
BfmI	CTRYAG	1 cut(s)	255
BisI	GCNGC	4 cut(s)	58, 61, 258, 261
BlsI	GCNGC	4 cut(s)	59, 62, 259, 262
Bme1390I	CCNGG	2 cut(s)	67, 119
BmiI	GGNNCC	2 cut(s)	96, 198
BmrFI	CCNGG	2 cut(s)	67, 119
BmsI	GCATC	1 cut(s)	234
BpuMI	CCSGG	2 cut(s)	67, 119
BsaHI	GRCGYC	1 cut(s)	6
BsaJI	CCNNGG	2 cut(s)	192, 225
BsaWI	WCCGGW	1 cut(s)	97
Bsc4I	CCNNNNNNNGG	3 cut(s)	73, 138, 193
BseDI	CCNNGG	2 cut(s)	192, 225
BseLI	CCNNNNNNNGG	3 cut(s)	73, 138, 193
BseRI	GAGGAG	2 cut(s)	50, 53
BseSI	GKGCMC	1 cut(s)	119
BseXI	GCAGC	4 cut(s)	69, 72, 247, 269
Bsh1236I	CGCG	2 cut(s)	45, 207
BsiHKAI	GWGCWC	1 cut(s)	146
BsiSI	CCGG	3 cut(s)	67, 98, 119
BslI	CCNNNNNNNGG	3 cut(s)	73, 138, 193
Bsp1286I	GDGCHC	2 cut(s)	119, 146
Bsp1407I	TGTACA	1 cut(s)	294
Bsp143I	GATC	2 cut(s)	160, 196
Bsp19I	CCATGG	1 cut(s)	225
BspACI	CCGC	3 cut(s)	45, 89, 156
BspFNI	CGCG	2 cut(s)	45, 207
BspLI	GGNNCC	2 cut(s)	96, 198
BspMAI	CTGCAG	1 cut(s)	259
BspPI	GGATC	3 cut(s)	155, 191, 204
BsrGI	TGTACA	1 cut(s)	294
BssECI	CCNNGG	2 cut(s)	192, 225
BssMI	GATC	2 cut(s)	160, 196
BssNI	GRCGYC	1 cut(s)	6
BssT1I	CCWWGG	2 cut(s)	192, 225
Bst4CI	ACNGT	1 cut(s)	53
Bst6I	CTCTTC	1 cut(s)	8
BstACI	GRCGYC	1 cut(s)	6
BstAUI	TGTACA	1 cut(s)	294
BstDSI	CCRYGG	1 cut(s)	225
BstFNI	CGCG	2 cut(s)	45, 207
BstHHI	GCGC	2 cut(s)	45, 209
BstKTI	GATC	2 cut(s)	163, 199
BstMBI	GATC	2 cut(s)	160, 196
BstSCI	CCNGG	2 cut(s)	65, 117
BstSFI	CTRYAG	1 cut(s)	255
BstSLI	GKGCMC	1 cut(s)	119
BstUI	CGCG	2 cut(s)	45, 207
BstV1I	GCAGC	4 cut(s)	69, 72, 247, 269
BstX2I	RGATCY	1 cut(s)	196
BstXI	CCANNNNNNTGG	1 cut(s)	226
BstYI	RGATCY	1 cut(s)	196
BtgI	CCRYGG	1 cut(s)	225
BtsI	GCAGTG	1 cut(s)	219
BtsIMutI	CAGTG	1 cut(s)	219
CfoI	GCGC	2 cut(s)	45, 209
Csp6I	GTAC	1 cut(s)	295
CviAII	CATG	2 cut(s)	226, 313
CviJI	RGCY	5 cut(s)	95, 106, 144, 260, 346
CviKI_1	RGCY	5 cut(s)	95, 106, 144, 260, 346
CviQI	GTAC	1 cut(s)	295
DpnI	GATC	2 cut(s)	162, 198
DpnII	GATC	2 cut(s)	160, 196
Eam1104I	CTCTTC	1 cut(s)	8
EarI	CTCTTC	1 cut(s)	8
EciI	GGCGGA	1 cut(s)	104
Ecl136II	GAGCTC	1 cut(s)	144
Eco130I	CCWWGG	2 cut(s)	192, 225
Eco24I	GRGCYC	1 cut(s)	146
Eco53kI	GAGCTC	1 cut(s)	144
EcoICRI	GAGCTC	1 cut(s)	144
EcoT14I	CCWWGG	2 cut(s)	192, 225
EcoT38I	GRGCYC	1 cut(s)	146
ErhI	CCWWGG	2 cut(s)	192, 225
FaeI	CATG	2 cut(s)	229, 316
FaiI	YATR	4 cut(s)	80, 227, 293, 314
FalI	AAGNNNNNCTT	1 cut(s)	330
FatI	CATG	2 cut(s)	225, 312
FauI	CCCGC	2 cut(s)	38, 163
Fnu4HI	GCNGC	4 cut(s)	58, 61, 258, 261
FriOI	GRGCYC	1 cut(s)	146
Fsp4HI	GCNGC	4 cut(s)	58, 61, 258, 261
GlaI	GCGC	2 cut(s)	44, 208
GluI	GCNGC	4 cut(s)	58, 61, 258, 261
HapII	CCGG	3 cut(s)	67, 98, 119
HhaI	GCGC	2 cut(s)	45, 209
Hin1I	GRCGYC	1 cut(s)	6
Hin1II	CATG	2 cut(s)	229, 316
Hin6I	GCGC	2 cut(s)	43, 207
HinP1I	GCGC	2 cut(s)	43, 207
HinfI	GANTC	2 cut(s)	26, 251
HpaII	CCGG	3 cut(s)	67, 98, 119
Hpy188I	TCNGA	1 cut(s)	328
Hpy188III	TCNNGA	2 cut(s)	10, 235
Hpy99I	CGWCG	4 cut(s)	11, 34, 131, 215
HpyCH4III	ACNGT	1 cut(s)	53
HpyCH4V	TGCA	2 cut(s)	257, 263
Hsp92I	GRCGYC	1 cut(s)	6
Hsp92II	CATG	2 cut(s)	229, 316
HspAI	GCGC	2 cut(s)	43, 207
Kzo9I	GATC	2 cut(s)	160, 196
LmnI	GCTCC	4 cut(s)	40, 100, 141, 149
LpnPI	CCDG	4 cut(s)	80, 111, 132, 261
Lsp1109I	GCAGC	4 cut(s)	69, 72, 247, 269
LweI	GCATC	1 cut(s)	234
MalI	GATC	2 cut(s)	162, 198
MboI	GATC	2 cut(s)	160, 196
MboII	GAAGA	1 cut(s)	25
MflI	RGATCY	1 cut(s)	196
MhlI	GDGCHC	2 cut(s)	119, 146
MluCI	AATT	1 cut(s)	265
MlyI	GAGTC	1 cut(s)	245
MnlI	CCTC	5 cut(s)	28, 31, 78, 85, 197
MslI	CAYNNNNRTG	1 cut(s)	224
MspA1I	CMGCKG	1 cut(s)	260
MspI	CCGG	3 cut(s)	67, 98, 119
MspR9I	CCNGG	2 cut(s)	67, 119
MvnI	CGCG	2 cut(s)	45, 207
NciI	CCSGG	2 cut(s)	67, 119
NcoI	CCATGG	1 cut(s)	225
NdeII	GATC	2 cut(s)	160, 196
NlaIII	CATG	2 cut(s)	229, 316
NlaIV	GGNNCC	2 cut(s)	96, 198
PcsI	WCGNNNNNNNCGW	1 cut(s)	180
PfeI	GAWTC	1 cut(s)	26
PflMI	CCANNNNNTGG	1 cut(s)	138
PkrI	GCNGC	4 cut(s)	59, 62, 259, 262
PleI	GAGTC	1 cut(s)	245
PpsI	GAGTC	1 cut(s)	245
Psp124BI	GAGCTC	1 cut(s)	146
PspN4I	GGNNCC	2 cut(s)	96, 198
PstI	CTGCAG	1 cut(s)	259
PsuI	RGATCY	1 cut(s)	196
PvuII	CAGCTG	1 cut(s)	260
RsaI	GTAC	1 cut(s)	296
RsaNI	GTAC	1 cut(s)	295
RseI	CAYNNNNRTG	1 cut(s)	224
SacI	GAGCTC	1 cut(s)	146
SatI	GCNGC	4 cut(s)	58, 61, 258, 261
Sau3AI	GATC	2 cut(s)	160, 196
SchI	GAGTC	1 cut(s)	245
ScrFI	CCNGG	2 cut(s)	67, 119
SduI	GDGCHC	2 cut(s)	119, 146
SetI	ASST	4 cut(s)	146, 262, 343, 348
SfaNI	GCATC	1 cut(s)	234
SfcI	CTRYAG	1 cut(s)	255
SmiMI	CAYNNNNRTG	1 cut(s)	224
Sse9I	AATT	1 cut(s)	265
SsiI	CCGC	3 cut(s)	45, 89, 156
SstI	GAGCTC	1 cut(s)	146
StyD4I	CCNGG	2 cut(s)	65, 117
StyI	CCWWGG	2 cut(s)	192, 225
TaaI	ACNGT	1 cut(s)	53
TaqI	TCGA	2 cut(s)	9, 29
TasI	AATT	1 cut(s)	265
TatI	WGTACW	1 cut(s)	294
TfiI	GAWTC	1 cut(s)	26
TscAI	CASTG	1 cut(s)	226
TseI	GCWGC	4 cut(s)	57, 60, 257, 260
TspDTI	ATGAA	2 cut(s)	125, 273
TspGWI	ACGGA	1 cut(s)	47
TspRI	CASTG	1 cut(s)	226
Van91I	CCANNNNNTGG	1 cut(s)	138
XapI	RAATTY	1 cut(s)	265

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.