Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0018284

Physical Location & Seq

Genomic Coordinates Forward (+)

1615159 .. 1617664

Gene Structure 2506 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1022300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 813 bp

ATGGGCGTCGAGAAGAGAGAGAGAGATTCGACGGAGGAGGAGCGCGGGAACAGCAGCAGCAGCAGTCGGATGGATACGGGGAGGGCGGAGGCTCCGGTATGGCTGATGAAGTGCCCGGTGGTCGTCGCCAAGTCGTGGAGCTCCCACAACCCTTCCGATCCCCACCCGATCGCCAAAGTCGTCCTCTCTTTTGACCCCCTCCGCGCCGACGACCCCTCTGCCATGGAGTTCAAGATGGAGATGCCTGGCTCCGCGGCTGCGAATTTGCCACAAAGTTATTCCTTGAATATGTTTAAAGACTTCGTTCCCATGTGCGTTTTTTCGGAAACAAATCAAGGCAAAGTTTCCGTGGAAGGTAAAGTGGATCACAAGTTTGATATGAAGCCTCACGGTACTAACATTGAGGAGTATGGGAAGCTGTGTCGTGAAAGGACAAACAAATCCATGATCAAGAACAGACAAATACAGATGATCGACAATGACCGAGGAGTACTTATGAGGCCCATGCCTGGGATGATCGGTTTGGTTTCATCCACCTCAAAGGATAAGAAGAAAACAGCACCGGTTAAACAATCAGACATGAAAAGGACTAGAAGAGATCGTGGGGAACTGGAGGATATAGTGTTCAAGTTATTTGAACAAAAACAACCAAACTGGACCTTGAAGCAGCTGGTGCAAGAAACCAATCAACCTGCGCAATTCTTGAAAGAGATACTGAATGAGCTCTGCGTTTACAACAAAAGGGGAACGAACCAGGGTACTTATGAGCTCAAGCCGGAATACAAGAAATCTGTTGAGGATACTGAAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 map03022

Pfam Domains

Protein Families

TFIIF_beta

Protein Analysis

271

Amino Acids

30.83

Weight (kDa)

8.52

Isoelectric Point (pI)

43.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TFIIF_beta_N	PF17683	32 - 143	1.5e-07	TFIIF, beta subunit N-terminus
TFIIF_beta	PF02270	198 - 262	3.9e-20	TFIIF, beta subunit HTH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0018284)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75510
fragaria_vesca	FvH4_2g38930
malus_domestica	MD08G1022300.v1.1 MD15G1019500.v1.1 MD15G1019600.v1.1
prunus_persica	Prupe.1G371700_v2.0.a1
pyrus_communis	pycom08g01720 pycom15g01620 pycom15g01630
rosa_multiflora	Rmu_sc0006892.1_g000007

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	696
Acc36I	ACCTGC	1 cut(s)	700
AccB7I	CCANNNNNTGG	1 cut(s)	135
AccII	CGCG	3 cut(s)	45, 204, 254
AciI	CCGC	5 cut(s)	45, 86, 202, 252, 254
AclWI	GGATC	2 cut(s)	152, 372
AcsI	RAATTY	1 cut(s)	262
AcyI	GRCGYC	1 cut(s)	6
AfaI	GTAC	3 cut(s)	394, 492, 760
AfiI	CCNNNNNNNGG	3 cut(s)	135, 509, 510
AgeI	ACCGGT	1 cut(s)	562
AgsI	TTSAA	6 cut(s)	232, 286, 628, 638, 664, 706
AjnI	CCWGG	3 cut(s)	244, 508, 753
AloI	GAACNNNNNNTCC	2 cut(s)	608, 640
AluBI	AGCT	5 cut(s)	141, 418, 670, 724, 769
AluI	AGCT	5 cut(s)	141, 418, 670, 724, 769
Alw21I	GWGCWC	3 cut(s)	143, 726, 771
AlwI	GGATC	2 cut(s)	152, 372
AoxI	GGCC	1 cut(s)	500
ApeKI	GCWGC	5 cut(s)	54, 57, 60, 257, 667
ApoI	RAATTY	1 cut(s)	262
AsiGI	ACCGGT	1 cut(s)	562
AspLEI	GCGC	3 cut(s)	45, 206, 697
AspS9I	GGNCC	2 cut(s)	501, 657
AsuC2I	CCSGG	1 cut(s)	116
AvaII	GGWCC	1 cut(s)	657
BaeGI	GKGCMC	1 cut(s)	116
BanII	GRGCYC	3 cut(s)	143, 726, 771
Bbv12I	GWGCWC	3 cut(s)	143, 726, 771
BbvI	GCAGC	5 cut(s)	66, 69, 72, 244, 679
BccI	CCATC	2 cut(s)	64, 229
BcgI	CGANNNNNNTGC	2 cut(s)	200, 234
BciT130I	CCWGG	3 cut(s)	246, 510, 755
BciVI	GTATCC	2 cut(s)	67, 793
BclI	TGATCA	1 cut(s)	447
BcnI	CCSGG	1 cut(s)	116
BfaI	CTAG	1 cut(s)	591
BfuAI	ACCTGC	1 cut(s)	700
BfuI	GTATCC	2 cut(s)	67, 793
BisI	GCNGC	6 cut(s)	55, 58, 61, 255, 258, 668
BlsI	GCNGC	6 cut(s)	56, 59, 62, 256, 259, 669
BmcAI	AGTACT	1 cut(s)	492
Bme1390I	CCNGG	4 cut(s)	116, 246, 510, 755
Bme18I	GGWCC	1 cut(s)	657
BmgT120I	GGNCC	2 cut(s)	501, 657
BmiI	GGNNCC	2 cut(s)	93, 250
BmrFI	CCNGG	4 cut(s)	116, 246, 510, 755
BmsI	GCATC	1 cut(s)	231
BpmI	CTGGAG	1 cut(s)	632
BpuEI	CTTGAG	1 cut(s)	755
BpuMI	CCSGG	1 cut(s)	116
BsaHI	GRCGYC	1 cut(s)	6
BsaJI	CCNNGG	6 cut(s)	222, 252, 348, 484, 509, 754
BsaWI	WCCGGW	2 cut(s)	94, 562
Bsc4I	CCNNNNNNNGG	3 cut(s)	135, 509, 510
Bse118I	RCCGGY	1 cut(s)	562
Bse1I	ACTGG	2 cut(s)	615, 659
BseBI	CCWGG	3 cut(s)	246, 510, 755
BseDI	CCNNGG	6 cut(s)	222, 252, 348, 484, 509, 754
BseGI	GGATG	3 cut(s)	75, 519, 530
BseLI	CCNNNNNNNGG	3 cut(s)	135, 509, 510
BseNI	ACTGG	2 cut(s)	615, 659
BseRI	GAGGAG	4 cut(s)	50, 53, 419, 501
BseSI	GKGCMC	1 cut(s)	116
BseXI	GCAGC	5 cut(s)	66, 69, 72, 244, 679
Bsh1236I	CGCG	3 cut(s)	45, 204, 254
Bsh1285I	CGRYCG	1 cut(s)	171
BshFI	GGCC	1 cut(s)	502
BshTI	ACCGGT	1 cut(s)	562
BsiEI	CGRYCG	1 cut(s)	171
BsiHKAI	GWGCWC	3 cut(s)	143, 726, 771
BsiSI	CCGG	4 cut(s)	95, 116, 563, 776
BslI	CCNNNNNNNGG	3 cut(s)	135, 509, 510
BsnI	GGCC	1 cut(s)	502
Bsp1286I	GDGCHC	4 cut(s)	116, 143, 726, 771
Bsp143I	GATC	7 cut(s)	157, 168, 364, 447, 471, 516, 598
Bsp19I	CCATGG	1 cut(s)	222
BspACI	CCGC	5 cut(s)	45, 86, 202, 252, 254
BspANI	GGCC	1 cut(s)	502
BspFNI	CGCG	3 cut(s)	45, 204, 254
BspLI	GGNNCC	2 cut(s)	93, 250
BspMI	ACCTGC	1 cut(s)	700
BspPI	GGATC	2 cut(s)	152, 372
BsrFI	RCCGGY	1 cut(s)	562
BsrI	ACTGG	2 cut(s)	615, 659
BssAI	RCCGGY	1 cut(s)	562
BssECI	CCNNGG	6 cut(s)	222, 252, 348, 484, 509, 754
BssMI	GATC	7 cut(s)	157, 168, 364, 447, 471, 516, 598
BssNI	GRCGYC	1 cut(s)	6
BssT1I	CCWWGG	1 cut(s)	222
Bst2UI	CCWGG	3 cut(s)	246, 510, 755
Bst4CI	ACNGT	1 cut(s)	392
Bst6I	CTCTTC	2 cut(s)	8, 589
BstACI	GRCGYC	1 cut(s)	6
BstAPI	GCANNNNNTGC	1 cut(s)	673
BstDSI	CCRYGG	3 cut(s)	222, 252, 348
BstF5I	GGATG	3 cut(s)	75, 519, 530
BstFNI	CGCG	3 cut(s)	45, 204, 254
BstHHI	GCGC	3 cut(s)	45, 206, 697
BstKTI	GATC	7 cut(s)	160, 171, 367, 450, 474, 519, 601
BstMBI	GATC	7 cut(s)	157, 168, 364, 447, 471, 516, 598
BstMCI	CGRYCG	1 cut(s)	171
BstMWI	GCNNNNNNNGC	3 cut(s)	51, 60, 673
BstNI	CCWGG	3 cut(s)	246, 510, 755
BstSCI	CCNGG	4 cut(s)	114, 244, 508, 753
BstSLI	GKGCMC	1 cut(s)	116
BstUI	CGCG	3 cut(s)	45, 204, 254
BstV1I	GCAGC	5 cut(s)	66, 69, 72, 244, 679
BsuI	GTATCC	2 cut(s)	67, 793
BsuRI	GGCC	1 cut(s)	502
BtgI	CCRYGG	3 cut(s)	222, 252, 348
BtsCI	GGATG	3 cut(s)	75, 519, 530
BveI	ACCTGC	1 cut(s)	700
CfoI	GCGC	3 cut(s)	45, 206, 697
Cfr10I	RCCGGY	1 cut(s)	562
Cfr13I	GGNCC	2 cut(s)	501, 657
Cfr42I	CCGCGG	1 cut(s)	255
Csp6I	GTAC	3 cut(s)	393, 491, 759
CspAI	ACCGGT	1 cut(s)	562
CviAII	CATG	5 cut(s)	223, 310, 445, 505, 580
CviQI	GTAC	3 cut(s)	393, 491, 759
DpnI	GATC	7 cut(s)	159, 170, 366, 449, 473, 518, 600
DpnII	GATC	7 cut(s)	157, 168, 364, 447, 471, 516, 598
DraI	TTTAAA	1 cut(s)	295
Eam1104I	CTCTTC	2 cut(s)	8, 589
EarI	CTCTTC	2 cut(s)	8, 589
EciI	GGCGGA	1 cut(s)	101
Ecl136II	GAGCTC	3 cut(s)	141, 724, 769
Eco130I	CCWWGG	1 cut(s)	222
Eco24I	GRGCYC	3 cut(s)	143, 726, 771
Eco47I	GGWCC	1 cut(s)	657
Eco53kI	GAGCTC	3 cut(s)	141, 724, 769
EcoICRI	GAGCTC	3 cut(s)	141, 724, 769
EcoRII	CCWGG	3 cut(s)	244, 508, 753
EcoT14I	CCWWGG	1 cut(s)	222
EcoT38I	GRGCYC	3 cut(s)	143, 726, 771
ErhI	CCWWGG	1 cut(s)	222
FaeI	CATG	5 cut(s)	226, 313, 448, 508, 583
FatI	CATG	5 cut(s)	222, 309, 444, 504, 579
FauI	CCCGC	1 cut(s)	38
FbaI	TGATCA	1 cut(s)	447
Fnu4HI	GCNGC	6 cut(s)	55, 58, 61, 255, 258, 668
FokI	GGATG	3 cut(s)	82, 517, 526
FriOI	GRGCYC	3 cut(s)	143, 726, 771
Fsp4HI	GCNGC	6 cut(s)	55, 58, 61, 255, 258, 668
FspBI	CTAG	1 cut(s)	591
FspI	TGCGCA	1 cut(s)	696
GlaI	GCGC	3 cut(s)	44, 205, 696
GluI	GCNGC	6 cut(s)	55, 58, 61, 255, 258, 668
GsuI	CTGGAG	1 cut(s)	632
HaeIII	GGCC	1 cut(s)	502
HapII	CCGG	4 cut(s)	95, 116, 563, 776
HhaI	GCGC	3 cut(s)	45, 206, 697
Hin1I	GRCGYC	1 cut(s)	6
Hin1II	CATG	5 cut(s)	226, 313, 448, 508, 583
Hin6I	GCGC	3 cut(s)	43, 204, 695
HinP1I	GCGC	3 cut(s)	43, 204, 695
HinfI	GANTC	1 cut(s)	26
HpaII	CCGG	4 cut(s)	95, 116, 563, 776
Hpy166II	GTNNAC	1 cut(s)	733
Hpy188I	TCNGA	4 cut(s)	69, 157, 325, 577
Hpy188III	TCNNGA	5 cut(s)	10, 232, 425, 451, 703
Hpy8I	GTNNAC	1 cut(s)	733
Hpy99I	CGWCG	4 cut(s)	11, 34, 128, 212
HpyAV	CCTTC	2 cut(s)	162, 347
HpyCH4III	ACNGT	1 cut(s)	392
HpyCH4V	TGCA	1 cut(s)	676
HpyF10VI	GCNNNNNNNGC	3 cut(s)	51, 60, 673
Hsp92I	GRCGYC	1 cut(s)	6
Hsp92II	CATG	5 cut(s)	226, 313, 448, 508, 583
HspAI	GCGC	3 cut(s)	43, 204, 695
Ksp22I	TGATCA	1 cut(s)	447
KspI	CCGCGG	1 cut(s)	255
Kzo9I	GATC	7 cut(s)	157, 168, 364, 447, 471, 516, 598
LmnI	GCTCC	5 cut(s)	40, 97, 138, 146, 254
Lsp1109I	GCAGC	5 cut(s)	66, 69, 72, 244, 679
LweI	GCATC	1 cut(s)	231
MaeI	CTAG	1 cut(s)	591
MalI	GATC	7 cut(s)	159, 170, 366, 449, 473, 518, 600
MboI	GATC	7 cut(s)	157, 168, 364, 447, 471, 516, 598
MboII	GAAGA	3 cut(s)	25, 562, 606
MhlI	GDGCHC	4 cut(s)	116, 143, 726, 771
MluCI	AATT	2 cut(s)	262, 698
MmeI	TCCRAC	1 cut(s)	47
MseI	TTAA	2 cut(s)	294, 567
MspA1I	CMGCKG	2 cut(s)	254, 670
MspI	CCGG	4 cut(s)	95, 116, 563, 776
MspR9I	CCNGG	4 cut(s)	116, 246, 510, 755
MvaI	CCWGG	3 cut(s)	246, 510, 755
MvnI	CGCG	3 cut(s)	45, 204, 254
MwoI	GCNNNNNNNGC	3 cut(s)	51, 60, 673
NciI	CCSGG	1 cut(s)	116
NcoI	CCATGG	1 cut(s)	222
NdeII	GATC	7 cut(s)	157, 168, 364, 447, 471, 516, 598
NlaIII	CATG	5 cut(s)	226, 313, 448, 508, 583
NlaIV	GGNNCC	2 cut(s)	93, 250
NsbI	TGCGCA	1 cut(s)	696
PcsI	WCGNNNNNNNCGW	1 cut(s)	177
PfeI	GAWTC	1 cut(s)	26
PflMI	CCANNNNNTGG	1 cut(s)	135
PinAI	ACCGGT	1 cut(s)	562
PkrI	GCNGC	6 cut(s)	56, 59, 62, 256, 259, 669
Ple19I	CGATCG	1 cut(s)	171
Psp124BI	GAGCTC	3 cut(s)	143, 726, 771
Psp6I	CCWGG	3 cut(s)	244, 508, 753
PspGI	CCWGG	3 cut(s)	244, 508, 753
PspN4I	GGNNCC	2 cut(s)	93, 250
PspPI	GGNCC	2 cut(s)	501, 657
PvuI	CGATCG	1 cut(s)	171
PvuII	CAGCTG	1 cut(s)	670
RsaI	GTAC	3 cut(s)	394, 492, 760
RsaNI	GTAC	3 cut(s)	393, 491, 759
SacI	GAGCTC	3 cut(s)	143, 726, 771
SacII	CCGCGG	1 cut(s)	255
SaqAI	TTAA	2 cut(s)	294, 567
SatI	GCNGC	6 cut(s)	55, 58, 61, 255, 258, 668
Sau3AI	GATC	7 cut(s)	157, 168, 364, 447, 471, 516, 598
Sau96I	GGNCC	2 cut(s)	501, 657
ScaI	AGTACT	1 cut(s)	492
ScrFI	CCNGG	4 cut(s)	116, 246, 510, 755
SduI	GDGCHC	4 cut(s)	116, 143, 726, 771
SetI	ASST	9 cut(s)	143, 358, 420, 539, 662, 672, 694, 726, 771
SfaNI	GCATC	1 cut(s)	231
Sfr303I	CCGCGG	1 cut(s)	255
SgrBI	CCGCGG	1 cut(s)	255
SinI	GGWCC	1 cut(s)	657
SmlI	CTYRAG	1 cut(s)	770
SmoI	CTYRAG	1 cut(s)	770
Sse9I	AATT	2 cut(s)	262, 698
SsiI	CCGC	5 cut(s)	45, 86, 202, 252, 254
SspMI	CTAG	1 cut(s)	591
SstI	GAGCTC	3 cut(s)	143, 726, 771
StyD4I	CCNGG	4 cut(s)	114, 244, 508, 753
StyI	CCWWGG	1 cut(s)	222
TaaI	ACNGT	1 cut(s)	392
TaqI	TCGA	3 cut(s)	9, 29, 474
TaqII	GACCGA	1 cut(s)	498
TasI	AATT	2 cut(s)	262, 698
TatI	WGTACW	1 cut(s)	490
TauI	GCSGC	1 cut(s)	257
TfiI	GAWTC	1 cut(s)	26
Tru1I	TTAA	2 cut(s)	294, 567
Tru9I	TTAA	2 cut(s)	294, 567
TseI	GCWGC	5 cut(s)	54, 57, 60, 257, 667
TspDTI	ATGAA	4 cut(s)	122, 395, 519, 596
TspGWI	ACGGA	2 cut(s)	47, 337
Van91I	CCANNNNNTGG	1 cut(s)	135
VpaK11BI	GGWCC	1 cut(s)	657
XapI	RAATTY	1 cut(s)	262
XspI	CTAG	1 cut(s)	591
ZrmI	AGTACT	1 cut(s)	492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.