AT2G01340

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011848
Physical Location & Seq
Forward (+)
163954 .. 165290
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G01340.1

Sequence Viewer

Length: 648 bp
ATGGGTAACAGTTTAGGCGGCAAGAAAACGACCAAAGTCATGAAGATAGACGGCGAGACTTTTAAACTGAAAACTCCGGTGACGGCTGAGGAGGTCTTAAAAGACTTTCCCGGCCACGTCTTGTTAGACTCCGAATCCGTCAAGCACTACGGTGCCCGAGCCAAACCACTTGAGGCGAAGCAGAGGTTGGAGGCGAAGCGGCTTTATTTTGTGGTGGAGCCGGTGAAAGAGTGTCCACCGAGAAGGGTAAGATCAGGAATTCACGTGAGTGCAAAGGAGAGGCTTGAGAGTCTCATGCTGGCCCGGAGATCGTCTTCCGATCTCTCCATACTCAAACCACCAGGAGGATGGACGACGGAGGAAGAAGAAGGAGCGGTGAGGAGGGTGAAGGTGAGGATTCCCAAAGCTGAGCTTGAAAAGCTCGTTAAAGAAGGAGCCACCGAAGCCGAAGCTACGCAGAAGATCGCTGCTCTCTTTATGGCCAAACAGAGGCAAGAGGAGGCATATCAGAACACACGACAGGACGAGCCCGCCACCACGGCCACCGCCACCGCCACCACCACCGCCACAAGAGGAGTCAAATCCCGTTTGAAACGAGTGAGTTTCATGGCGGAAAGAGGAGGAGGGAGCGAGATCACTGTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

215

Amino Acids

23.7

Weight (kDa)

9.75

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 161 6.1e-21 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71015 AT1G71015 AT2G01340
fragaria_vesca FvH4_4g32310
malus_domestica MD16G1029200.v1.1
prunus_persica Prupe.1G323600_v2.0.a1
pyrus_communis pycom13g02480 pycom16g02510
rosa_chinensis RchiOBHm_Chr4g0441101
rosa_laevigata RLG00000006114
rosa_multiflora Rmu_sc0006317.1_g000016 Rmu_sc0030601.1_g000001
rosa_roxburghii Rroxscaffold_5G00382070
rosa_rugosa Rorug04G0331100
rosa_samantha Rh4AG382700 Rh4BG396500 Rh4CG411600 Rh4DG389300
rosa_wichuraiana Rw4G033030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 152
AccBSI CCGCTC 1 cut(s) 374
AciI CCGC 8 cut(s) 18, 199, 374, 531, 546, 552, 564, 611
AcoI YGGCCR 3 cut(s) 112, 480, 540
AcsI RAATTY 1 cut(s) 258
AcvI CACGTG 1 cut(s) 265
AfiI CCNNNNNNNGG 2 cut(s) 344, 489
AgsI TTSAA 2 cut(s) 416, 592
AjiI CACGTC 1 cut(s) 118
AjnI CCWGG 1 cut(s) 340
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AleI CACNNNNGTG 2 cut(s) 150, 267
AluBI AGCT 5 cut(s) 407, 412, 421, 452, 644
AluI AGCT 5 cut(s) 407, 412, 421, 452, 644
Alw26I GTCTC 2 cut(s) 50, 296
Ama87I CYCGRG 1 cut(s) 156
AoxI GGCC 4 cut(s) 112, 300, 480, 540
ApeKI GCWGC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 301
AsuC2I CCSGG 2 cut(s) 111, 304
AsuHPI GGTGA 5 cut(s) 91, 235, 388, 397, 403
AvaI CYCGRG 1 cut(s) 156
BaeGI GKGCMC 1 cut(s) 157
BalI TGGCCA 1 cut(s) 482
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 531
BbrPI CACGTG 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 87
BbvI GCAGC 1 cut(s) 454
BccI CCATC 1 cut(s) 342
BceAI ACGGC 3 cut(s) 67, 99, 555
BciT130I CCWGG 1 cut(s) 342
BcnI CCSGG 2 cut(s) 111, 304
BcoDI GTCTC 2 cut(s) 50, 296
BfmI CTRYAG 1 cut(s) 639
BglI GCCNNNNNGGC 1 cut(s) 539
BisI GCNGC 3 cut(s) 19, 200, 468
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 3 cut(s) 20, 201, 469
Bme1390I CCNGG 3 cut(s) 111, 304, 342
BmeT110I CYCGRG 1 cut(s) 156
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 3 cut(s) 154, 219, 436
BmrFI CCNGG 3 cut(s) 111, 304, 342
BoxI GACNNNNGTC 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 87
Bpu1102I GCTNAGC 1 cut(s) 408
BpuEI CTTGAG 2 cut(s) 191, 305
BpuMI CCSGG 2 cut(s) 111, 304
BsaAI YACGTR 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 537
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 2 cut(s) 344, 489
Bse118I RCCGGY 1 cut(s) 220
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 537
BseGI GGATG 1 cut(s) 353
BseLI CCNNNNNNNGG 2 cut(s) 344, 489
BseMII CTCAG 2 cut(s) 78, 399
BseRI GAGGAG 6 cut(s) 104, 394, 512, 588, 633, 636
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 1 cut(s) 454
BshFI GGCC 4 cut(s) 114, 302, 482, 542
BshNI GGYRCC 1 cut(s) 152
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 4 cut(s) 77, 111, 221, 304
BslI CCNNNNNNNGG 2 cut(s) 344, 489
BsmAI GTCTC 2 cut(s) 50, 296
BsnI GGCC 4 cut(s) 114, 302, 482, 542
BsoBI CYCGRG 1 cut(s) 156
Bsp1286I GDGCHC 2 cut(s) 157, 531
Bsp143I GATC 5 cut(s) 251, 308, 319, 462, 633
Bsp1720I GCTNAGC 1 cut(s) 408
BspACI CCGC 8 cut(s) 18, 199, 374, 531, 546, 552, 564, 611
BspANI GGCC 4 cut(s) 114, 302, 482, 542
BspCNI CTCAG 2 cut(s) 79, 400
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 3 cut(s) 154, 219, 436
BspT107I GGYRCC 1 cut(s) 152
BsrBI CCGCTC 1 cut(s) 374
BsrFI RCCGGY 1 cut(s) 220
BssAI RCCGGY 1 cut(s) 220
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 5 cut(s) 251, 308, 319, 462, 633
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 3 cut(s) 11, 152, 640
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 2 cut(s) 300, 531
BstDEI CTNAG 2 cut(s) 87, 408
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 1 cut(s) 353
BstKTI GATC 5 cut(s) 254, 311, 322, 465, 636
BstMAI GTCTC 2 cut(s) 50, 296
BstMBI GATC 5 cut(s) 251, 308, 319, 462, 633
BstMWI GCNNNNNNNGC 3 cut(s) 418, 443, 539
BstNI CCWGG 1 cut(s) 342
BstPAI GACNNNNGTC 1 cut(s) 35
BstSCI CCNGG 3 cut(s) 109, 302, 340
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 454
BstV2I GAAGAC 1 cut(s) 306
BstXI CCANNNNNNTGG 1 cut(s) 348
BsuRI GGCC 4 cut(s) 114, 302, 482, 542
BtgI CCRYGG 1 cut(s) 537
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 1 cut(s) 353
BtsIMutI CAGTG 1 cut(s) 636
Cac8I GCNNGC 2 cut(s) 300, 531
CciI TCATGA 1 cut(s) 39
Cfr10I RCCGGY 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 301
CviAII CATG 3 cut(s) 40, 295, 607
DdeI CTNAG 2 cut(s) 87, 408
DpnI GATC 5 cut(s) 253, 310, 321, 464, 635
DpnII GATC 5 cut(s) 251, 308, 319, 462, 633
DraI TTTAAA 1 cut(s) 64
EaeI YGGCCR 3 cut(s) 112, 480, 540
EciI GGCGGA 1 cut(s) 626
Eco24I GRGCYC 1 cut(s) 531
Eco72I CACGTG 1 cut(s) 265
Eco88I CYCGRG 1 cut(s) 156
EcoRI GAATTC 1 cut(s) 258
EcoRII CCWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 531
FaeI CATG 3 cut(s) 43, 298, 610
FaiI YATR 6 cut(s) 41, 296, 329, 479, 505, 608
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FatI CATG 3 cut(s) 39, 294, 606
FauI CCCGC 1 cut(s) 538
Fnu4HI GCNGC 3 cut(s) 19, 200, 468
FokI GGATG 1 cut(s) 360
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 3 cut(s) 19, 200, 468
GluI GCNGC 3 cut(s) 19, 200, 468
HaeIII GGCC 4 cut(s) 114, 302, 482, 542
HapII CCGG 4 cut(s) 77, 111, 221, 304
Hin1II CATG 3 cut(s) 43, 298, 610
HinfI GANTC 5 cut(s) 128, 134, 289, 397, 576
HpaII CCGG 4 cut(s) 77, 111, 221, 304
HphI GGTGA 5 cut(s) 91, 235, 388, 397, 403
Hpy166II GTNNAC 1 cut(s) 236
Hpy188I TCNGA 3 cut(s) 133, 319, 510
Hpy188III TCNNGA 2 cut(s) 40, 255
Hpy8I GTNNAC 1 cut(s) 236
Hpy99I CGWCG 1 cut(s) 358
HpyAV CCTTC 4 cut(s) 237, 362, 382, 425
HpyCH4III ACNGT 3 cut(s) 11, 152, 640
HpyCH4IV ACGT 2 cut(s) 117, 264
HpyCH4V TGCA 1 cut(s) 272
HpyF10VI GCNNNNNNNGC 3 cut(s) 418, 443, 539
HpyF3I CTNAG 2 cut(s) 87, 408
HpySE526I ACGT 2 cut(s) 117, 264
Hsp92II CATG 3 cut(s) 43, 298, 610
Kzo9I GATC 5 cut(s) 251, 308, 319, 462, 633
LmnI GCTCC 4 cut(s) 217, 371, 434, 627
LpnPI CCDG 9 cut(s) 90, 124, 234, 240, 284, 317, 327, 354, 506
Lsp1109I GCAGC 1 cut(s) 454
MaeII ACGT 2 cut(s) 117, 264
MaeIII GTNAC 2 cut(s) 5, 79
MalI GATC 5 cut(s) 253, 310, 321, 464, 635
MbiI CCGCTC 1 cut(s) 374
MboI GATC 5 cut(s) 251, 308, 319, 462, 633
MboII GAAGA 5 cut(s) 55, 306, 374, 377, 472
MhlI GDGCHC 2 cut(s) 157, 531
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 1 cut(s) 258
MluNI TGGCCA 1 cut(s) 482
MlyI GAGTC 3 cut(s) 122, 298, 585
MmeI TCCRAC 1 cut(s) 168
Mox20I TGGCCA 1 cut(s) 482
MscI TGGCCA 1 cut(s) 482
MseI TTAA 3 cut(s) 63, 98, 426
MslI CAYNNNNRTG 2 cut(s) 150, 267
Msp20I TGGCCA 1 cut(s) 482
MspI CCGG 4 cut(s) 77, 111, 221, 304
MspR9I CCNGG 3 cut(s) 111, 304, 342
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 3 cut(s) 418, 443, 539
NciI CCSGG 2 cut(s) 111, 304
NdeII GATC 5 cut(s) 251, 308, 319, 462, 633
NlaIII CATG 3 cut(s) 43, 298, 610
NlaIV GGNNCC 3 cut(s) 154, 219, 436
NmuCI GTSAC 1 cut(s) 79
OliI CACNNNNGTG 2 cut(s) 150, 267
PagI TCATGA 1 cut(s) 39
PfeI GAWTC 2 cut(s) 134, 397
PkrI GCNGC 3 cut(s) 20, 201, 469
PleI GAGTC 3 cut(s) 122, 297, 584
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 3 cut(s) 122, 297, 584
Ppu21I YACGTR 1 cut(s) 265
PshAI GACNNNNGTC 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 340
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 3 cut(s) 154, 219, 436
PspPI GGNCC 1 cut(s) 301
RseI CAYNNNNRTG 2 cut(s) 150, 267
SaqAI TTAA 3 cut(s) 63, 98, 426
SatI GCNGC 3 cut(s) 19, 200, 468
Sau3AI GATC 5 cut(s) 251, 308, 319, 462, 633
Sau96I GGNCC 1 cut(s) 301
SchI GAGTC 3 cut(s) 122, 298, 585
ScrFI CCNGG 3 cut(s) 111, 304, 342
SduI GDGCHC 2 cut(s) 157, 531
SfcI CTRYAG 1 cut(s) 639
SmiMI CAYNNNNRTG 2 cut(s) 150, 267
SmlI CTYRAG 2 cut(s) 170, 284
SmoI CTYRAG 2 cut(s) 170, 284
Sse9I AATT 1 cut(s) 258
SsiI CCGC 8 cut(s) 18, 199, 374, 531, 546, 552, 564, 611
StyD4I CCNGG 3 cut(s) 109, 302, 340
TaaI ACNGT 3 cut(s) 11, 152, 640
TaiI ACGT 2 cut(s) 120, 267
TasI AATT 1 cut(s) 258
TauI GCSGC 2 cut(s) 21, 202
TfiI GAWTC 2 cut(s) 134, 397
Tru1I TTAA 3 cut(s) 63, 98, 426
Tru9I TTAA 3 cut(s) 63, 98, 426
TscAI CASTG 1 cut(s) 643
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 1 cut(s) 467
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 56, 595
TspGWI ACGGA 2 cut(s) 127, 371
TspRI CASTG 1 cut(s) 643
XapI RAATTY 1 cut(s) 258
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.