FvH4_4g32310

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011848
Physical Location & Seq
Forward (+)
31018295 .. 31020661
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32310.t1

Sequence Viewer

Length: 630 bp
ATGGGAAACAGTTTGGGAAGCAAAAAGAAAGCAAAGGTTATGCAAATTAGTGGGGAGACATTGAAGTTGAAGACGCCTGTCCAAGCTGGTCAGGTAATGAAGAACTACCCTGGTCACGTTTTGCTTAAATCCCAAGAGGTAAAGCATTTAGGGATACGAGCAAGGCCTTTGGAGCCACACCAACACCTTGAGGCCAAGGGAGTCTACTTCCTTGTGGAGTTGCCAAAGGCAGCTGCAGCTCATAGTTTTAGTGACAAAGTTCCGAGGAGAGTCCGGTCCGGAATAAACATGACTGCCAAGGACAGGCTTGAGAGTTTAATGCTGTCTCGGAGGTCGGTTTCCGACCTCTCCATGATGAGATCGATACCCGAGAAATCTCATCAAGATGGGCCGGTGAGGGTGAGGATGAGGCTTCCCAAGGCTCAAGTTGAGATGCTGATGCAGCAGAGCAGAAATGAGGAAGAGGCGGCAGAGAAGATTGTGGATCTCTGCATGGTTAATGCCAACATTGGTGGTGAGTTCAATGAGAAGGATGGTCATGGAAAAGTGGTTAAACAAGCCACCAAGATGCGTGAGAAGCGAGTGAGCTTCCGAAATGAAGGAGAAATACAAATAGCTGTGGGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

210

Amino Acids

23.36

Weight (kDa)

10.03

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 183 3.2e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71015 AT1G71015 AT2G01340
fragaria_vesca FvH4_4g32310
malus_domestica MD16G1029200.v1.1
prunus_persica Prupe.1G323600_v2.0.a1
pyrus_communis pycom13g02480 pycom16g02510
rosa_chinensis RchiOBHm_Chr4g0441101
rosa_laevigata RLG00000006114
rosa_multiflora Rmu_sc0006317.1_g000016 Rmu_sc0030601.1_g000001
rosa_roxburghii Rroxscaffold_5G00382070
rosa_rugosa Rorug04G0331100
rosa_samantha Rh4AG382700 Rh4BG396500 Rh4CG411600 Rh4DG389300
rosa_wichuraiana Rw4G033030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 204
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 1 cut(s) 467
AclWI GGATC 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 303
AgsI TTSAA 3 cut(s) 64, 70, 523
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 5 cut(s) 86, 233, 239, 588, 617
AluI AGCT 5 cut(s) 86, 233, 239, 588, 617
Alw26I GTCTC 2 cut(s) 50, 330
AlwI GGATC 1 cut(s) 492
Ama87I CYCGRG 1 cut(s) 368
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 3 cut(s) 164, 192, 389
ApeKI GCWGC 4 cut(s) 230, 233, 236, 442
AspS9I GGNCC 2 cut(s) 276, 389
AsuHPI GGTGA 3 cut(s) 406, 412, 527
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 276
BarI GAAGNNNNNNTAC 2 cut(s) 591, 623
BbsI GAAGAC 1 cut(s) 77
BbvI GCAGC 4 cut(s) 220, 242, 248, 454
BccI CCATC 2 cut(s) 380, 527
BciT130I CCWGG 1 cut(s) 111
BciVI GTATCC 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 50, 330
BfmI CTRYAG 1 cut(s) 234
BfuI GTATCC 1 cut(s) 147
BisI GCNGC 5 cut(s) 231, 234, 237, 443, 468
BlsI GCNGC 5 cut(s) 232, 235, 238, 444, 469
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 276
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 2 cut(s) 276, 389
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 3 cut(s) 423, 429, 558
BoxI GACNNNNGTC 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 77
BpuEI CTTGAG 3 cut(s) 209, 329, 408
Bsa29I ATCGAT 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 74
BsaJI CCNNGG 5 cut(s) 109, 195, 263, 297, 417
BsaWI WCCGGW 2 cut(s) 273, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse118I RCCGGY 1 cut(s) 391
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 362
BseDI CCNNGG 5 cut(s) 109, 195, 263, 297, 417
BseGI GGATG 2 cut(s) 411, 538
BseLI CCNNNNNNNGG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 280
BseXI GCAGC 4 cut(s) 220, 242, 248, 454
BshFI GGCC 3 cut(s) 166, 194, 391
BshVI ATCGAT 1 cut(s) 362
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 3 cut(s) 274, 279, 392
BslI CCNNNNNNNGG 1 cut(s) 303
BsmAI GTCTC 2 cut(s) 50, 330
BsnI GGCC 3 cut(s) 166, 194, 391
BsoBI CYCGRG 1 cut(s) 368
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 2 cut(s) 359, 484
BspACI CCGC 1 cut(s) 467
BspANI GGCC 3 cut(s) 166, 194, 391
BspDI ATCGAT 1 cut(s) 362
BspEI TCCGGA 1 cut(s) 278
BspLI GGNNCC 1 cut(s) 174
BspMAI CTGCAG 1 cut(s) 238
BspPI GGATC 1 cut(s) 492
BsrFI RCCGGY 1 cut(s) 391
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 5 cut(s) 109, 195, 263, 297, 417
BssMI GATC 2 cut(s) 359, 484
BssNI GRCGYC 1 cut(s) 74
BssT1I CCWWGG 3 cut(s) 195, 297, 417
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 11
Bst6I CTCTTC 1 cut(s) 456
BstACI GRCGYC 1 cut(s) 74
BstF5I GGATG 2 cut(s) 411, 538
BstKTI GATC 2 cut(s) 362, 487
BstMAI GTCTC 2 cut(s) 50, 330
BstMBI GATC 2 cut(s) 359, 484
BstMWI GCNNNNNNNGC 4 cut(s) 172, 236, 442, 577
BstNI CCWGG 1 cut(s) 111
BstPAI GACNNNNGTC 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 109
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 4 cut(s) 220, 242, 248, 454
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 362
BsuI GTATCC 1 cut(s) 147
BsuRI GGCC 3 cut(s) 166, 194, 391
BsuTUI ATCGAT 1 cut(s) 362
BtsCI GGATG 2 cut(s) 411, 538
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 276, 389
ClaI ATCGAT 1 cut(s) 362
CpoI CGGWCCG 1 cut(s) 276
CseI GACGC 1 cut(s) 82
CspCI CAANNNNNGTGG 2 cut(s) 493, 528
CspI CGGWCCG 1 cut(s) 276
CviAII CATG 4 cut(s) 289, 352, 493, 539
DpnI GATC 2 cut(s) 361, 486
DpnII GATC 2 cut(s) 359, 484
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco130I CCWWGG 3 cut(s) 195, 297, 417
Eco147I AGGCCT 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 3 cut(s) 195, 297, 417
ErhI CCWWGG 3 cut(s) 195, 297, 417
FaeI CATG 4 cut(s) 292, 355, 496, 542
FaiI YATR 6 cut(s) 41, 243, 290, 353, 494, 540
FatI CATG 4 cut(s) 288, 351, 492, 538
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 5 cut(s) 231, 234, 237, 443, 468
FokI GGATG 2 cut(s) 418, 545
Fsp4HI GCNGC 5 cut(s) 231, 234, 237, 443, 468
GluI GCNGC 5 cut(s) 231, 234, 237, 443, 468
HaeIII GGCC 3 cut(s) 166, 194, 391
HapII CCGG 3 cut(s) 274, 279, 392
HgaI GACGC 1 cut(s) 82
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 4 cut(s) 292, 355, 496, 542
HinfI GANTC 2 cut(s) 201, 270
HpaII CCGG 3 cut(s) 274, 279, 392
HphI GGTGA 3 cut(s) 406, 412, 527
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 4 cut(s) 264, 330, 343, 593
Hpy188III TCNNGA 2 cut(s) 279, 383
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 2 cut(s) 523, 593
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 4 cut(s) 43, 236, 442, 492
HpyF10VI GCNNNNNNNGC 4 cut(s) 172, 236, 442, 577
HpySE526I ACGT 1 cut(s) 117
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 4 cut(s) 292, 355, 496, 542
Kpn2I TCCGGA 1 cut(s) 278
Kzo9I GATC 2 cut(s) 359, 484
LmnI GCTCC 1 cut(s) 172
LpnPI CCDG 9 cut(s) 72, 77, 90, 96, 123, 287, 289, 292, 405
Lsp1109I GCAGC 4 cut(s) 220, 242, 248, 454
LweI GCATC 3 cut(s) 423, 429, 558
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 113, 251
MalI GATC 2 cut(s) 361, 486
MboI GATC 2 cut(s) 359, 484
MboII GAAGA 4 cut(s) 82, 112, 473, 487
MflI RGATCY 1 cut(s) 484
MluCI AATT 1 cut(s) 45
MlyI GAGTC 2 cut(s) 210, 279
MmeI TCCRAC 1 cut(s) 366
MroI TCCGGA 1 cut(s) 278
MseI TTAA 5 cut(s) 126, 317, 498, 552, 628
MslI CAYNNNNRTG 2 cut(s) 384, 566
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 3 cut(s) 274, 279, 392
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MwoI GCNNNNNNNGC 4 cut(s) 172, 236, 442, 577
NdeII GATC 2 cut(s) 359, 484
NlaIII CATG 4 cut(s) 292, 355, 496, 542
NlaIV GGNNCC 1 cut(s) 174
NmuCI GTSAC 2 cut(s) 113, 251
PceI AGGCCT 1 cut(s) 166
PkrI GCNGC 5 cut(s) 232, 235, 238, 444, 469
PleI GAGTC 2 cut(s) 209, 278
PpsI GAGTC 2 cut(s) 209, 278
PshAI GACNNNNGTC 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 2 cut(s) 276, 389
PstI CTGCAG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 484
PvuII CAGCTG 1 cut(s) 233
RseI CAYNNNNRTG 2 cut(s) 384, 566
Rsr2I CGGWCCG 1 cut(s) 276
RsrII CGGWCCG 1 cut(s) 276
SaqAI TTAA 5 cut(s) 126, 317, 498, 552, 628
SatI GCNGC 5 cut(s) 231, 234, 237, 443, 468
Sau3AI GATC 2 cut(s) 359, 484
Sau96I GGNCC 2 cut(s) 276, 389
SchI GAGTC 2 cut(s) 210, 279
ScrFI CCNGG 1 cut(s) 111
SfaNI GCATC 3 cut(s) 423, 429, 558
SfcI CTRYAG 1 cut(s) 234
SinI GGWCC 1 cut(s) 276
SmiMI CAYNNNNRTG 2 cut(s) 384, 566
SmlI CTYRAG 3 cut(s) 188, 308, 423
SmoI CTYRAG 3 cut(s) 188, 308, 423
Sse9I AATT 1 cut(s) 45
SseBI AGGCCT 1 cut(s) 166
SsiI CCGC 1 cut(s) 467
StuI AGGCCT 1 cut(s) 166
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 3 cut(s) 195, 297, 417
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 1 cut(s) 120
TaqI TCGA 1 cut(s) 362
TasI AATT 1 cut(s) 45
TauI GCSGC 1 cut(s) 470
Tru1I TTAA 5 cut(s) 126, 317, 498, 552, 628
Tru9I TTAA 5 cut(s) 126, 317, 498, 552, 628
TseFI GTSAC 2 cut(s) 113, 251
TseI GCWGC 4 cut(s) 230, 233, 236, 442
Tsp45I GTSAC 2 cut(s) 113, 251
TspDTI ATGAA 2 cut(s) 113, 612
VpaK11BI GGWCC 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.