pycom13g02480

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011848
Physical Location & Seq
Reverse (-)
1713398 .. 1715084
1687 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g02480.1

Sequence Viewer

Length: 687 bp
ATGGGAAACAGCTTGGGAGGCAAAAAACGTGCAAAAGTTATGCAAGTTAATGGAGAGACATTCAAGTTGAAAACACCAGTGCAAGCTGGTGAGGTTGTCAAGGATTTCCCAGGTCATGTTTTGCTAAAATCATCGGATGTGAAGCATTTCGGGGTTCGGGCAAAGCCGTTGGAACTCTACCAACAGCTTGAGCCAAACAGGTTGTATTTTCTTGTGGAGTTGCCAAAGGTTGATGTTAAAAAAGAGAGTTTTCCGAGAAGGGTTCGTTCGGGAATAAACATGAGTGCTAAGGACAGGCTCGAAAGCCTAATGCTTTCTCGGAGGTCGGTTTCGGACCTTACGGTCATGAAATCATCGGCATCGACAGCAAGCATTGTACCCGAGGAGGAGGCGAATGGGGCGGTGCGCGTGAGGATGAGGCTTCCGAGGGCAGAAGTGGAGAAATTGATGCGGCAAAGTGGAGACGACGCGGAGGCAGCTCAGAAGATTATGGATCTTTGCATGGTGAACACCAACAATGTGAAGAGCAATGATCAATGTTTGGGTTTGAACCCGCAAATACATTGGAAGGATGAAGGAAGATTGCTCGAGTCGCAAGGAATCAAGGGGCGTCATCGGGTTCAGAAACGAGTGAGTTTCCGACCAGCCAATGAAGGAGAGATACAGCAAATAGTGGCGGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

229

Amino Acids

25.52

Weight (kDa)

9.85

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 215 1.2e-21 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71015 AT1G71015 AT2G01340
fragaria_vesca FvH4_4g32310
malus_domestica MD16G1029200.v1.1
prunus_persica Prupe.1G323600_v2.0.a1
pyrus_communis pycom13g02480 pycom16g02510
rosa_chinensis RchiOBHm_Chr4g0441101
rosa_laevigata RLG00000006114
rosa_multiflora Rmu_sc0006317.1_g000016 Rmu_sc0030601.1_g000001
rosa_roxburghii Rroxscaffold_5G00382070
rosa_rugosa Rorug04G0331100
rosa_samantha Rh4AG382700 Rh4BG396500 Rh4CG411600 Rh4DG389300
rosa_wichuraiana Rw4G033030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AccII CGCG 2 cut(s) 408, 470
AciI CCGC 5 cut(s) 401, 451, 470, 554, 677
AclWI GGATC 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 610
AfaI GTAC 1 cut(s) 378
AgsI TTSAA 3 cut(s) 64, 70, 550
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 4 cut(s) 12, 86, 187, 479
AluI AGCT 4 cut(s) 12, 86, 187, 479
Alw26I GTCTC 2 cut(s) 50, 456
AlwI GGATC 1 cut(s) 501
Ama87I CYCGRG 2 cut(s) 380, 587
ApeKI GCWGC 1 cut(s) 476
Asp700I GAANNNNTTC 1 cut(s) 146
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 1 cut(s) 334
AsuHPI GGTGA 2 cut(s) 101, 517
AvaI CYCGRG 2 cut(s) 380, 587
AvaII GGWCC 1 cut(s) 334
BarI GAAGNNNNNNTAC 2 cut(s) 645, 677
BbvI GCAGC 1 cut(s) 488
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 111
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 2 cut(s) 50, 456
BisI GCNGC 3 cut(s) 452, 477, 678
BlsI GCNGC 3 cut(s) 453, 478, 679
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 334
BmeT110I CYCGRG 2 cut(s) 380, 587
BmgT120I GGNCC 1 cut(s) 334
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 2 cut(s) 368, 438
Bpu10I CCTNAGC 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 610
BsaJI CCNNGG 3 cut(s) 109, 381, 425
Bse1I ACTGG 1 cut(s) 77
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 3 cut(s) 109, 381, 425
BseGI GGATG 3 cut(s) 142, 420, 577
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 2 cut(s) 398, 401
BseXI GCAGC 1 cut(s) 488
Bsh1236I CGCG 2 cut(s) 408, 470
BsiHKCI CYCGRG 2 cut(s) 380, 587
BsmAI GTCTC 2 cut(s) 50, 456
BsmBI CGTCTC 1 cut(s) 456
BsoBI CYCGRG 2 cut(s) 380, 587
Bsp143I GATC 2 cut(s) 493, 532
BspACI CCGC 5 cut(s) 401, 451, 470, 554, 677
BspCNI CTCAG 1 cut(s) 493
BspFNI CGCG 2 cut(s) 408, 470
BspHI TCATGA 1 cut(s) 345
BspPI GGATC 1 cut(s) 501
BspQI GCTCTTC 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 535
BsrI ACTGG 1 cut(s) 77
BssECI CCNNGG 3 cut(s) 109, 381, 425
BssMI GATC 2 cut(s) 493, 532
BssNI GRCGYC 1 cut(s) 610
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 343
Bst6I CTCTTC 1 cut(s) 518
BstACI GRCGYC 1 cut(s) 610
BstC8I GCNNGC 2 cut(s) 84, 370
BstDEI CTNAG 2 cut(s) 288, 480
BstF5I GGATG 3 cut(s) 142, 420, 577
BstFNI CGCG 2 cut(s) 408, 470
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 2 cut(s) 496, 535
BstMAI GTCTC 2 cut(s) 50, 456
BstMBI GATC 2 cut(s) 493, 532
BstMWI GCNNNNNNNGC 5 cut(s) 18, 365, 398, 476, 592
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 109
BstUI CGCG 2 cut(s) 408, 470
BstV1I GCAGC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BtsCI GGATG 3 cut(s) 142, 420, 577
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 84, 370
CciI TCATGA 1 cut(s) 345
CfoI GCGC 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 334
CseI GACGC 2 cut(s) 476, 599
Csp6I GTAC 1 cut(s) 377
CviAII CATG 4 cut(s) 116, 280, 346, 502
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 2 cut(s) 288, 480
DpnI GATC 2 cut(s) 495, 534
DpnII GATC 2 cut(s) 493, 532
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
Eam1104I CTCTTC 1 cut(s) 518
EarI CTCTTC 1 cut(s) 518
Eco47I GGWCC 1 cut(s) 334
Eco88I CYCGRG 2 cut(s) 380, 587
EcoRII CCWGG 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 456
FaeI CATG 4 cut(s) 119, 283, 349, 505
FaiI YATR 6 cut(s) 41, 117, 281, 347, 491, 503
FatI CATG 4 cut(s) 115, 279, 345, 501
FauI CCCGC 1 cut(s) 561
FbaI TGATCA 1 cut(s) 532
Fnu4HI GCNGC 3 cut(s) 452, 477, 678
FokI GGATG 3 cut(s) 149, 427, 584
Fsp4HI GCNGC 3 cut(s) 452, 477, 678
GlaI GCGC 1 cut(s) 407
GluI GCNGC 3 cut(s) 452, 477, 678
HgaI GACGC 2 cut(s) 476, 599
HhaI GCGC 1 cut(s) 408
Hin1I GRCGYC 1 cut(s) 610
Hin1II CATG 4 cut(s) 119, 283, 349, 505
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HinfI GANTC 2 cut(s) 590, 600
HphI GGTGA 2 cut(s) 101, 517
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 8 cut(s) 136, 255, 321, 334, 426, 483, 624, 641
Hpy188III TCNNGA 2 cut(s) 270, 346
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 4 cut(s) 252, 562, 569, 647
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 4 cut(s) 32, 43, 82, 501
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 365, 398, 476, 592
HpyF3I CTNAG 2 cut(s) 288, 480
HpySE526I ACGT 1 cut(s) 28
Hsp92I GRCGYC 1 cut(s) 610
Hsp92II CATG 4 cut(s) 119, 283, 349, 505
HspAI GCGC 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 2 cut(s) 493, 532
LguI GCTCTTC 1 cut(s) 518
LpnPI CCDG 7 cut(s) 72, 90, 96, 123, 184, 280, 657
Lsp1109I GCAGC 1 cut(s) 488
LweI GCATC 2 cut(s) 368, 438
MaeII ACGT 1 cut(s) 28
MalI GATC 2 cut(s) 495, 534
MboI GATC 2 cut(s) 493, 532
MboII GAAGA 3 cut(s) 496, 535, 591
MflI RGATCY 1 cut(s) 493
MluCI AATT 1 cut(s) 443
MlyI GAGTC 1 cut(s) 599
MmeI TCCRAC 2 cut(s) 150, 664
MroXI GAANNNNTTC 1 cut(s) 146
MseI TTAA 3 cut(s) 48, 237, 685
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 2 cut(s) 408, 470
MwoI GCNNNNNNNGC 5 cut(s) 18, 365, 398, 476, 592
NdeII GATC 2 cut(s) 493, 532
NlaIII CATG 4 cut(s) 119, 283, 349, 505
PaeR7I CTCGAG 1 cut(s) 587
PagI TCATGA 1 cut(s) 345
PciSI GCTCTTC 1 cut(s) 518
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 1 cut(s) 600
PkrI GCNGC 3 cut(s) 453, 478, 679
PleI GAGTC 1 cut(s) 598
PpsI GAGTC 1 cut(s) 598
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspPI GGNCC 1 cut(s) 334
PspXI VCTCGAGB 1 cut(s) 587
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SapI GCTCTTC 1 cut(s) 518
SaqAI TTAA 3 cut(s) 48, 237, 685
SatI GCNGC 3 cut(s) 452, 477, 678
Sau3AI GATC 2 cut(s) 493, 532
Sau96I GGNCC 1 cut(s) 334
SchI GAGTC 1 cut(s) 599
ScrFI CCNGG 1 cut(s) 111
SfaNI GCATC 2 cut(s) 368, 438
Sfr274I CTCGAG 1 cut(s) 587
SinI GGWCC 1 cut(s) 334
SlaI CTCGAG 1 cut(s) 587
SmlI CTYRAG 2 cut(s) 188, 587
SmoI CTYRAG 2 cut(s) 188, 587
Sse9I AATT 1 cut(s) 443
SsiI CCGC 5 cut(s) 401, 451, 470, 554, 677
StyD4I CCNGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 343
TaiI ACGT 1 cut(s) 31
TaqI TCGA 3 cut(s) 300, 362, 588
TasI AATT 1 cut(s) 443
TauI GCSGC 2 cut(s) 454, 680
TfiI GAWTC 1 cut(s) 600
Tru1I TTAA 3 cut(s) 48, 237, 685
Tru9I TTAA 3 cut(s) 48, 237, 685
TscAI CASTG 1 cut(s) 84
TseI GCWGC 1 cut(s) 476
TspDTI ATGAA 3 cut(s) 362, 588, 666
TspRI CASTG 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 334
XhoI CTCGAG 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.