RLG00000006114

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011848
Physical Location & Seq
Reverse (-)
4486520 .. 4488024
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006114

Sequence Viewer

Length: 636 bp
ATGGGCAACAGTTTGGGAAGCAAAAAGAAAGCAAAGGTTCTGCAAATTAGTGGGGAGACATTGAAGTTGAAGACGCCGGTCCAAGCTGGTGAAGTAGTGAAGGACTACCCTGGTCACGTTTTGCTTAAATCCCAAGAGGTAAAGCATTTGGGGATCCGAGCAAAGCCTTTGGAGCCACACCAACACCTTGAGGCCAAGGGAGTCTACTTCCTTGTGGAGTTGCCAAAGGCTAGTAGTACTAGTGACAATCCAAAAGAGAGAGTTCCGAGGAGAGTCCGGTCCGGAATACACATGACTGCCAAGGACAGGCTTGAGAGTTTAATGCTGTCTCGGAGGTCGGTTTCCGACCTCTCCACCATGAAATCGATACCCGAGAAATCGCAAGATGGGCCGGTGCGCGTGAGGATGAGGCTTCCCAAGGCTCAAGTTGAGATGCTAATGCAGCAGAGCAGAGATAAGGAAGAGGCAGCAGAGAAGATTATGGATATGTGCATGGTTAATGCCAACATTGGTGGTGAGACCAATGAGAAGTATGGTCATGGAAGAGTGGTTAGACAAGGCACCAAGACGCGTGAGAAGCGAGTGAGCTTCCGACCGGTCGATGAAGGAGAAATACAAATAGCTGTGCCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

212

Amino Acids

23.6

Weight (kDa)

9.89

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 199 5.3e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71015 AT1G71015 AT2G01340
fragaria_vesca FvH4_4g32310
malus_domestica MD16G1029200.v1.1
prunus_persica Prupe.1G323600_v2.0.a1
pyrus_communis pycom13g02480 pycom16g02510
rosa_chinensis RchiOBHm_Chr4g0441101
rosa_laevigata RLG00000006114
rosa_multiflora Rmu_sc0006317.1_g000016 Rmu_sc0030601.1_g000001
rosa_roxburghii Rroxscaffold_5G00382070
rosa_rugosa Rorug04G0331100
rosa_samantha Rh4AG382700 Rh4BG396500 Rh4CG411600 Rh4DG389300
rosa_wichuraiana Rw4G033030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 560
AccI GTMKAC 1 cut(s) 204
AccII CGCG 2 cut(s) 399, 571
AccIII TCCGGA 1 cut(s) 281
AclWI GGATC 2 cut(s) 148, 161
AcyI GRCGYC 1 cut(s) 74
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 306
AflIII ACRYGT 1 cut(s) 569
AgeI ACCGGT 1 cut(s) 595
AgsI TTSAA 2 cut(s) 64, 70
AhlI ACTAGT 1 cut(s) 239
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 3 cut(s) 86, 588, 623
AluI AGCT 3 cut(s) 86, 588, 623
Alw26I GTCTC 3 cut(s) 50, 333, 512
AlwI GGATC 2 cut(s) 148, 161
Ama87I CYCGRG 1 cut(s) 371
Aor13HI TCCGGA 1 cut(s) 281
AoxI GGCC 2 cut(s) 192, 389
ApeKI GCWGC 2 cut(s) 442, 467
AsiGI ACCGGT 1 cut(s) 595
AspLEI GCGC 1 cut(s) 399
AspS9I GGNCC 3 cut(s) 79, 279, 389
AsuHPI GGTGA 2 cut(s) 101, 527
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 2 cut(s) 79, 279
BamHI GGATCC 1 cut(s) 153
BanI GGYRCC 1 cut(s) 560
BarI GAAGNNNNNNTAC 2 cut(s) 597, 629
BbsI GAAGAC 1 cut(s) 77
BbvI GCAGC 2 cut(s) 454, 479
BccI CCATC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 111
BcoDI GTCTC 3 cut(s) 50, 333, 512
BcuI ACTAGT 1 cut(s) 239
BfaI CTAG 2 cut(s) 231, 240
BisI GCNGC 2 cut(s) 443, 468
BlsI GCNGC 2 cut(s) 444, 469
BmcAI AGTACT 1 cut(s) 238
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 2 cut(s) 79, 279
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 3 cut(s) 79, 279, 389
BmiI GGNNCC 3 cut(s) 155, 174, 562
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 423
BoxI GACNNNNGTC 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 77
BpuEI CTTGAG 3 cut(s) 209, 332, 408
Bsa29I ATCGAT 1 cut(s) 365
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 5 cut(s) 109, 195, 266, 300, 417
BsaWI WCCGGW 3 cut(s) 276, 281, 595
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse118I RCCGGY 3 cut(s) 76, 391, 595
BseAI TCCGGA 1 cut(s) 281
BseBI CCWGG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 365
BseDI CCNNGG 5 cut(s) 109, 195, 266, 300, 417
BseGI GGATG 1 cut(s) 411
BseLI CCNNNNNNNGG 1 cut(s) 306
BseRI GAGGAG 1 cut(s) 283
BseXI GCAGC 2 cut(s) 454, 479
Bsh1236I CGCG 2 cut(s) 399, 571
Bsh1285I CGRYCG 2 cut(s) 596, 600
BshFI GGCC 2 cut(s) 194, 391
BshNI GGYRCC 1 cut(s) 560
BshTI ACCGGT 1 cut(s) 595
BshVI ATCGAT 1 cut(s) 365
BsiEI CGRYCG 2 cut(s) 596, 600
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 5 cut(s) 77, 277, 282, 392, 596
BslI CCNNNNNNNGG 1 cut(s) 306
BsmAI GTCTC 3 cut(s) 50, 333, 512
BsnI GGCC 2 cut(s) 194, 391
Bso31I GGTCTC 1 cut(s) 512
BsoBI CYCGRG 1 cut(s) 371
Bsp13I TCCGGA 1 cut(s) 281
Bsp143I GATC 1 cut(s) 153
BspANI GGCC 2 cut(s) 194, 391
BspDI ATCGAT 1 cut(s) 365
BspEI TCCGGA 1 cut(s) 281
BspFNI CGCG 2 cut(s) 399, 571
BspLI GGNNCC 3 cut(s) 155, 174, 562
BspPI GGATC 2 cut(s) 148, 161
BspT107I GGYRCC 1 cut(s) 560
BspTNI GGTCTC 1 cut(s) 512
BsrFI RCCGGY 3 cut(s) 76, 391, 595
BssAI RCCGGY 3 cut(s) 76, 391, 595
BssECI CCNNGG 5 cut(s) 109, 195, 266, 300, 417
BssMI GATC 1 cut(s) 153
BssNI GRCGYC 1 cut(s) 74
BssT1I CCWWGG 3 cut(s) 195, 300, 417
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 11
Bst6I CTCTTC 2 cut(s) 456, 538
BstACI GRCGYC 1 cut(s) 74
BstF5I GGATG 1 cut(s) 411
BstFNI CGCG 2 cut(s) 399, 571
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 3 cut(s) 50, 333, 512
BstMBI GATC 1 cut(s) 153
BstMCI CGRYCG 2 cut(s) 596, 600
BstMWI GCNNNNNNNGC 4 cut(s) 172, 388, 442, 577
BstNI CCWGG 1 cut(s) 111
BstPAI GACNNNNGTC 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 109
BstUI CGCG 2 cut(s) 399, 571
BstV1I GCAGC 2 cut(s) 454, 479
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
Bsu15I ATCGAT 1 cut(s) 365
BsuRI GGCC 2 cut(s) 194, 391
BsuTUI ATCGAT 1 cut(s) 365
BtsCI GGATG 1 cut(s) 411
CfoI GCGC 1 cut(s) 399
Cfr10I RCCGGY 3 cut(s) 76, 391, 595
Cfr13I GGNCC 3 cut(s) 79, 279, 389
ClaI ATCGAT 1 cut(s) 365
CpoI CGGWCCG 1 cut(s) 279
CseI GACGC 2 cut(s) 82, 577
Csp6I GTAC 1 cut(s) 237
CspAI ACCGGT 1 cut(s) 595
CspCI CAANNNNNGTGG 2 cut(s) 493, 528
CspI CGGWCCG 1 cut(s) 279
CviAII CATG 4 cut(s) 292, 358, 493, 539
CviQI GTAC 1 cut(s) 237
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 456, 538
EarI CTCTTC 2 cut(s) 456, 538
Eco130I CCWWGG 3 cut(s) 195, 300, 417
Eco31I GGTCTC 1 cut(s) 512
Eco47I GGWCC 2 cut(s) 79, 279
Eco88I CYCGRG 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 3 cut(s) 195, 300, 417
ErhI CCWWGG 3 cut(s) 195, 300, 417
FaeI CATG 4 cut(s) 295, 361, 496, 542
FaiI YATR 7 cut(s) 293, 359, 482, 488, 494, 534, 540
FatI CATG 4 cut(s) 291, 357, 492, 538
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 443, 468
FokI GGATG 1 cut(s) 418
Fsp4HI GCNGC 2 cut(s) 443, 468
FspBI CTAG 2 cut(s) 231, 240
GlaI GCGC 1 cut(s) 398
GluI GCNGC 2 cut(s) 443, 468
HaeIII GGCC 2 cut(s) 194, 391
HapII CCGG 5 cut(s) 77, 277, 282, 392, 596
HgaI GACGC 2 cut(s) 82, 577
HhaI GCGC 1 cut(s) 399
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 4 cut(s) 295, 361, 496, 542
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HinfI GANTC 2 cut(s) 201, 273
HpaII CCGG 5 cut(s) 77, 277, 282, 392, 596
HphI GGTGA 2 cut(s) 101, 527
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 5 cut(s) 158, 267, 333, 346, 593
Hpy188III TCNNGA 1 cut(s) 282
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 2 cut(s) 94, 599
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 3 cut(s) 43, 442, 492
HpyF10VI GCNNNNNNNGC 4 cut(s) 172, 388, 442, 577
HpySE526I ACGT 1 cut(s) 117
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 4 cut(s) 295, 361, 496, 542
HspAI GCGC 1 cut(s) 397
Kpn2I TCCGGA 1 cut(s) 281
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 172
LpnPI CCDG 9 cut(s) 72, 90, 96, 123, 290, 292, 295, 405, 609
Lsp1109I GCAGC 2 cut(s) 454, 479
LweI GCATC 1 cut(s) 423
MaeI CTAG 2 cut(s) 231, 240
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 113, 242
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 4 cut(s) 82, 473, 487, 555
MflI RGATCY 1 cut(s) 153
MluCI AATT 1 cut(s) 45
MluI ACGCGT 1 cut(s) 569
MlyI GAGTC 2 cut(s) 210, 282
MmeI TCCRAC 2 cut(s) 369, 616
MnlI CCTC 8 cut(s) 130, 184, 261, 327, 359, 396, 402, 457
MroI TCCGGA 1 cut(s) 281
MseI TTAA 4 cut(s) 126, 320, 498, 634
MspI CCGG 5 cut(s) 77, 277, 282, 392, 596
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 2 cut(s) 399, 571
MwoI GCNNNNNNNGC 4 cut(s) 172, 388, 442, 577
NdeII GATC 1 cut(s) 153
NlaIII CATG 4 cut(s) 295, 361, 496, 542
NlaIV GGNNCC 3 cut(s) 155, 174, 562
NmuCI GTSAC 2 cut(s) 113, 242
PinAI ACCGGT 1 cut(s) 595
PkrI GCNGC 2 cut(s) 444, 469
PleI GAGTC 2 cut(s) 209, 281
PpsI GAGTC 2 cut(s) 209, 281
PshAI GACNNNNGTC 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 3 cut(s) 155, 174, 562
PspPI GGNCC 3 cut(s) 79, 279, 389
PsuI RGATCY 1 cut(s) 153
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
Rsr2I CGGWCCG 1 cut(s) 279
RsrII CGGWCCG 1 cut(s) 279
SaqAI TTAA 4 cut(s) 126, 320, 498, 634
SatI GCNGC 2 cut(s) 443, 468
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 3 cut(s) 79, 279, 389
ScaI AGTACT 1 cut(s) 238
SchI GAGTC 2 cut(s) 210, 282
ScrFI CCNGG 1 cut(s) 111
SetI ASST 9 cut(s) 39, 88, 120, 141, 189, 338, 351, 590, 625
SfaNI GCATC 1 cut(s) 423
SinI GGWCC 2 cut(s) 79, 279
SmlI CTYRAG 3 cut(s) 188, 311, 423
SmoI CTYRAG 3 cut(s) 188, 311, 423
SpeI ACTAGT 1 cut(s) 239
Sse9I AATT 1 cut(s) 45
SspMI CTAG 2 cut(s) 231, 240
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 3 cut(s) 195, 300, 417
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 1 cut(s) 120
TaqI TCGA 2 cut(s) 365, 600
TasI AATT 1 cut(s) 45
TatI WGTACW 1 cut(s) 236
Tru1I TTAA 4 cut(s) 126, 320, 498, 634
Tru9I TTAA 4 cut(s) 126, 320, 498, 634
TseFI GTSAC 2 cut(s) 113, 242
TseI GCWGC 2 cut(s) 442, 467
Tsp45I GTSAC 2 cut(s) 113, 242
TspDTI ATGAA 2 cut(s) 374, 618
VpaK11BI GGWCC 2 cut(s) 79, 279
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 2 cut(s) 231, 240
ZrmI AGTACT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.