MD16G1029200.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011848
Physical Location & Seq
Reverse (-)
2082095 .. 2084343
2249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1029200.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGGAAACAGCTTGGGAGGCAAAAAACGTGCAAAAGTTATGCAAGTTAATGGAGAGACATTCAAGTTGAAAACACCAGTGCAAGCTGGTGAGGTTGTCAAGGATTTCCCAGGTCATGTTTTGCTAAAATCATCGGATGTGAAGCATTTCGGGGTTCGGGCAAAGCCGTTGGAACCCTACCAACAGCTTGAGCCAAACAGGTTGTATTTTCTTGTGGAGTTGCCCAAGGTTGATGTTAAAAAAGAGAGTTTTCCGAGAAGGGTTCGTTCTGGAATAAACATGAGTGCTAAGGACAGGCTCGAAAGCCTAATGCTTTCTCGGAGGTCGGTTTCGGACCTTTCGGTCATGAAATCATCGGCATCGACAGCAAGCATTGTGCCCGAGGAGGAGGCGAATGGGGCGGTGCGCGTGAGGATGAGGCTTCCGAGGGCAGAAGTGGAGAAATTGATGCGGCAAAGTAGAGACGACGGGGAGGCAGCTCAGCAGATTATGGATCTTTGCATGGTGAACACCAACAACGTGAAGGGCAATGATCAAGGTTTGGGTTTGAACCCGCAAATGCATTGGAAGGATGGAGGAAGATTGCTTGGGTCGCAAGGAATCAAGGGGCGTGATCGGATTCAGAAACGAGTGAGTTTCCGACCAGCTAATGAAGGAGAGGTCTTAACCAGTAGAGATGAGCAGCGCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

231

Amino Acids

25.75

Weight (kDa)

10.03

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 200 7.5e-23 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71015 AT1G71015 AT2G01340
fragaria_vesca FvH4_4g32310
malus_domestica MD16G1029200.v1.1
prunus_persica Prupe.1G323600_v2.0.a1
pyrus_communis pycom13g02480 pycom16g02510
rosa_chinensis RchiOBHm_Chr4g0441101
rosa_laevigata RLG00000006114
rosa_multiflora Rmu_sc0006317.1_g000016 Rmu_sc0030601.1_g000001
rosa_roxburghii Rroxscaffold_5G00382070
rosa_rugosa Rorug04G0331100
rosa_samantha Rh4AG382700 Rh4BG396500 Rh4CG411600 Rh4DG389300
rosa_wichuraiana Rw4G033030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AccII CGCG 1 cut(s) 408
AciI CCGC 3 cut(s) 401, 451, 554
AclWI GGATC 1 cut(s) 501
AgsI TTSAA 3 cut(s) 64, 70, 550
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 5 cut(s) 12, 86, 187, 479, 647
AluI AGCT 5 cut(s) 12, 86, 187, 479, 647
Alw26I GTCTC 2 cut(s) 50, 456
AlwI GGATC 1 cut(s) 501
AlwNI CAGNNNCTG 1 cut(s) 688
Ama87I CYCGRG 1 cut(s) 380
ApeKI GCWGC 2 cut(s) 476, 682
Asp700I GAANNNNTTC 1 cut(s) 146
AspLEI GCGC 2 cut(s) 408, 687
AspS9I GGNCC 1 cut(s) 334
AsuHPI GGTGA 2 cut(s) 101, 517
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 1 cut(s) 334
BaeGI GKGCMC 1 cut(s) 381
BbvI GCAGC 1 cut(s) 488
BccI CCATC 1 cut(s) 566
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 111
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 2 cut(s) 50, 456
BfmI CTRYAG 1 cut(s) 689
BfoI RGCGCY 1 cut(s) 688
BisI GCNGC 3 cut(s) 452, 477, 683
BlpI GCTNAGC 1 cut(s) 480
BlsI GCNGC 3 cut(s) 453, 478, 684
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 334
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 334
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 2 cut(s) 368, 438
Bpu10I CCTNAGC 1 cut(s) 288
Bpu1102I GCTNAGC 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 209
BsaJI CCNNGG 4 cut(s) 109, 225, 381, 425
Bse1I ACTGG 2 cut(s) 77, 669
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 4 cut(s) 109, 225, 381, 425
BseGI GGATG 3 cut(s) 142, 420, 577
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 2 cut(s) 77, 669
BseRI GAGGAG 2 cut(s) 398, 401
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 1 cut(s) 488
Bsh1236I CGCG 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 380
BsmAI GTCTC 2 cut(s) 50, 456
BsmBI CGTCTC 1 cut(s) 456
BsoBI CYCGRG 1 cut(s) 380
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 3 cut(s) 493, 532, 613
Bsp1720I GCTNAGC 1 cut(s) 480
BspACI CCGC 3 cut(s) 401, 451, 554
BspCNI CTCAG 1 cut(s) 493
BspFNI CGCG 1 cut(s) 408
BspHI TCATGA 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 174
BspPI GGATC 1 cut(s) 501
BsrDI GCAATG 1 cut(s) 535
BsrI ACTGG 2 cut(s) 77, 669
BssECI CCNNGG 4 cut(s) 109, 225, 381, 425
BssMI GATC 3 cut(s) 493, 532, 613
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 84, 370
BstDEI CTNAG 2 cut(s) 288, 480
BstF5I GGATG 3 cut(s) 142, 420, 577
BstFNI CGCG 1 cut(s) 408
BstH2I RGCGCY 1 cut(s) 688
BstHHI GCGC 2 cut(s) 408, 687
BstKTI GATC 3 cut(s) 496, 535, 616
BstMAI GTCTC 2 cut(s) 50, 456
BstMBI GATC 3 cut(s) 493, 532, 613
BstMWI GCNNNNNNNGC 4 cut(s) 18, 365, 398, 592
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 109
BstSFI CTRYAG 1 cut(s) 689
BstSLI GKGCMC 1 cut(s) 381
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 488
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BtsCI GGATG 3 cut(s) 142, 420, 577
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 84, 370
CaiI CAGNNNCTG 1 cut(s) 688
CciI TCATGA 1 cut(s) 345
CfoI GCGC 2 cut(s) 408, 687
Cfr13I GGNCC 1 cut(s) 334
CviAII CATG 4 cut(s) 116, 280, 346, 502
DdeI CTNAG 2 cut(s) 288, 480
DpnI GATC 3 cut(s) 495, 534, 615
DpnII GATC 3 cut(s) 493, 532, 613
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 334
Eco88I CYCGRG 1 cut(s) 380
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 225
EcoT22I ATGCAT 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 456
FaeI CATG 4 cut(s) 119, 283, 349, 505
FaiI YATR 6 cut(s) 41, 117, 281, 347, 491, 503
FatI CATG 4 cut(s) 115, 279, 345, 501
FauI CCCGC 1 cut(s) 561
FbaI TGATCA 1 cut(s) 532
Fnu4HI GCNGC 3 cut(s) 452, 477, 683
FokI GGATG 3 cut(s) 149, 427, 584
Fsp4HI GCNGC 3 cut(s) 452, 477, 683
GlaI GCGC 2 cut(s) 407, 686
GluI GCNGC 3 cut(s) 452, 477, 683
HaeII RGCGCY 1 cut(s) 688
HhaI GCGC 2 cut(s) 408, 687
Hin1II CATG 4 cut(s) 119, 283, 349, 505
Hin6I GCGC 2 cut(s) 406, 685
HinP1I GCGC 2 cut(s) 406, 685
HinfI GANTC 2 cut(s) 600, 619
HphI GGTGA 2 cut(s) 101, 517
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 8 cut(s) 136, 255, 321, 334, 426, 618, 624, 641
Hpy188III TCNNGA 2 cut(s) 270, 346
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 4 cut(s) 252, 517, 562, 647
HpyCH4IV ACGT 2 cut(s) 28, 519
HpyCH4V TGCA 5 cut(s) 32, 43, 82, 501, 562
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 365, 398, 592
HpyF3I CTNAG 2 cut(s) 288, 480
HpySE526I ACGT 2 cut(s) 28, 519
Hsp92II CATG 4 cut(s) 119, 283, 349, 505
HspAI GCGC 2 cut(s) 406, 685
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 3 cut(s) 493, 532, 613
LpnPI CCDG 9 cut(s) 72, 90, 96, 123, 184, 255, 280, 657, 682
Lsp1109I GCAGC 1 cut(s) 488
LweI GCATC 2 cut(s) 368, 438
MaeII ACGT 2 cut(s) 28, 519
MalI GATC 3 cut(s) 495, 534, 615
MboI GATC 3 cut(s) 493, 532, 613
MboII GAAGA 1 cut(s) 591
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 1 cut(s) 443
MmeI TCCRAC 2 cut(s) 150, 664
Mph1103I ATGCAT 1 cut(s) 564
MroXI GAANNNNTTC 1 cut(s) 146
MseI TTAA 3 cut(s) 48, 237, 665
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 4 cut(s) 18, 365, 398, 592
NdeII GATC 3 cut(s) 493, 532, 613
NlaIII CATG 4 cut(s) 119, 283, 349, 505
NlaIV GGNNCC 1 cut(s) 174
NsiI ATGCAT 1 cut(s) 564
PagI TCATGA 1 cut(s) 345
PdmI GAANNNNTTC 1 cut(s) 146
PfeI GAWTC 2 cut(s) 600, 619
PkrI GCNGC 3 cut(s) 453, 478, 684
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 1 cut(s) 334
PstNI CAGNNNCTG 1 cut(s) 688
PsuI RGATCY 1 cut(s) 493
SaqAI TTAA 3 cut(s) 48, 237, 665
SatI GCNGC 3 cut(s) 452, 477, 683
Sau3AI GATC 3 cut(s) 493, 532, 613
Sau96I GGNCC 1 cut(s) 334
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 1 cut(s) 381
SfaNI GCATC 2 cut(s) 368, 438
SfcI CTRYAG 1 cut(s) 689
SinI GGWCC 1 cut(s) 334
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 1 cut(s) 443
SsiI CCGC 3 cut(s) 401, 451, 554
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 1 cut(s) 225
TaiI ACGT 2 cut(s) 31, 522
TaqI TCGA 2 cut(s) 300, 362
TaqII GACCGA 1 cut(s) 331
TasI AATT 1 cut(s) 443
TauI GCSGC 1 cut(s) 454
TfiI GAWTC 2 cut(s) 600, 619
Tru1I TTAA 3 cut(s) 48, 237, 665
Tru9I TTAA 3 cut(s) 48, 237, 665
TscAI CASTG 1 cut(s) 84
TseI GCWGC 2 cut(s) 476, 682
TspDTI ATGAA 2 cut(s) 362, 666
TspRI CASTG 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 334
XmnI GAANNNNTTC 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.