AT2G04850

Cytochrome b-561 / ferric reductase transmembrane domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012003
Physical Location & Seq
Forward (+)
1704111 .. 1705778
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04850.1

Sequence Viewer

Length: 1215 bp
ATGGCGACTTTGATTCTATCATTCTTGCTTCTTCTTCTAGCAACAAAGCTTCCTGAATCACTAGCTGGTCACTGCACCACCACGACGGCGACGAAAAGCTTCGAGAAATGTATCTCTCTTCCAACACAACAAGCGTCAATCGCGTGGACATACCATCCACACAACGCTACTCTCGACCTTTGTTTCTTTGGAACTTTCATCTCACCTTCCGGGTGGGTTGGATGGGGGATTAACCCGGATTCCCCGGCGCAGATGACTGGTTCTCGTGTCCTCATCGCCTTTCCAGATCCGAATTCTGGTCAGCTCATCCTCCTTCCTTACGTTCTTGACTCTTCAGTCAAGCTTCAGAAAGGTCCTCTGCTTTCCCGTCCGCTTGACCTTGTCCGTCTCTCTTCCTCCTCTGCTTCTCTCTACGGTGGTAAAATGGCCACTATCCGTAATGGTGCTTCCGTTCAGATTTATGCCTCTGTTAAGCTCTCCTCGAACAATACTAAGATCCACCATGTTTGGAACAGAGGTCTTTATGTCCAAGGTTACTCTCCCACCATTCATCCTACTACTTCCACCGATCTCTCCTCCTTCTCCACCTTTGATGTCACTTCAGGATTTGCAACTGTTAATCAAAACAGCGGTTCCCGAGCTTTAAAAGTGACTCACGGAGTAGTCAACGCGATATCGTGGGGTTTTCTCCTCCCGGCGGGAGCAGTAACAGCTAGATACCTCCGCCAAATGCAATCAATAGGACCCACTTGGTTCTACATCCACGCAGCAATACAGCTAACCGGTTTCCTCCTAGGAACCATTGGCTTCTCCATTGGAATCGTGCTCGGCCATAATTCCCCTGGAGTGACCTATGGGTTACATCGGAGCCTCGGGATAGCTACTTTCACAGCAGCGGCGCTGCAAACACTGGCTTTGCTGTTCAGACCAAAGACGACCAATAAGTTCCGGAGGTACTGGAAGTCTTACCATCATTTTGTCGGGTACGCGTGTGTGGTGATGGGCGTGGTGAATGTGTTCCAAGGGTTTGAGGTTTTAAGGGAAGGAAGGTCGTACGCAAAGCTCGGTTACTGTCTATGTCTGTCGACGCTGGTTGGTGTCTGCGTGGCCATGGAAGTTAACTCGTGGGTTGTGTTTTGTCGAAAGGCTAAAGAGGAGAAGATGAAGAGAGATGGTTTGACCGGTGTCGATAGATGCAGTGGGAGTCATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000293 GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0016723 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

404

Amino Acids

43.88

Weight (kDa)

9.65

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 85 - 198 1.3e-19 Protein of unknown function (DUF568)
Cytochrom_B561 PF03188 248 - 343 1.2e-08 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04850
fragaria_vesca FvH4_6g41290
malus_domestica MD09G1120800.v1.1 MD17G1111800.v1.1
prunus_persica Prupe.3G202700_v2.0.a1
pyrus_communis pycom09g04540 pycom17g10370
rosa_chinensis RchiOBHm_Chr2g0156851
rosa_laevigata RLG00000020946
rosa_multiflora Rmu_co8482377.1_g000001 Rmu_sc0008149.1_g000001 Rmu_ssc0000286.1_g000024
rosa_roxburghii Rroxscaffold_2G00092670
rosa_rugosa Rorug02G0456600
rosa_samantha Rh2AG522200 Rh2BG535600 Rh2CG507100 Rh2DG544600
rosa_wichuraiana Rw2G043120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
AccI GTMKAC 1 cut(s) 1085
AccII CGCG 3 cut(s) 143, 671, 989
AccIII TCCGGA 1 cut(s) 948
AciI CCGC 5 cut(s) 371, 630, 698, 724, 896
AclWI GGATC 2 cut(s) 281, 490
AcoI YGGCCR 3 cut(s) 426, 829, 1107
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 3 cut(s) 318, 329, 585
AfaI GTAC 3 cut(s) 956, 986, 1055
AfiI CCNNNNNNNGG 2 cut(s) 296, 697
AflIII ACRYGT 1 cut(s) 987
AgeI ACCGGT 2 cut(s) 782, 1181
AjnI CCWGG 1 cut(s) 841
Alw21I GWGCWC 1 cut(s) 828
Alw26I GTCTC 1 cut(s) 392
AlwI GGATC 2 cut(s) 281, 490
Ama87I CYCGRG 2 cut(s) 636, 872
Aor13HI TCCGGA 1 cut(s) 948
AoxI GGCC 3 cut(s) 426, 829, 1107
ApeKI GCWGC 3 cut(s) 767, 893, 901
ApoI RAATTY 1 cut(s) 292
AsiGI ACCGGT 2 cut(s) 782, 1181
Asp700I GAANNNNTTC 2 cut(s) 98, 1016
AspA2I CCTAGG 1 cut(s) 793
AspLEI GCGC 2 cut(s) 250, 901
AspS9I GGNCC 2 cut(s) 353, 743
AsuC2I CCSGG 4 cut(s) 211, 236, 245, 695
AsuHPI GGTGA 3 cut(s) 195, 1009, 1021
AvaI CYCGRG 2 cut(s) 636, 872
AvaII GGWCC 2 cut(s) 353, 743
AvrII CCTAGG 1 cut(s) 793
BaeI ACNNNNGTAYC 2 cut(s) 976, 1009
BalI TGGCCA 2 cut(s) 428, 1109
BarI GAAGNNNNNNTAC 2 cut(s) 430, 462
BauI CACGAG 2 cut(s) 264, 1123
Bbv12I GWGCWC 1 cut(s) 828
BbvI GCAGC 3 cut(s) 779, 888, 905
BccI CCATC 5 cut(s) 162, 216, 978, 994, 1166
BceAI ACGGC 1 cut(s) 102
BciT130I CCWGG 1 cut(s) 843
BcnI CCSGG 4 cut(s) 211, 236, 245, 695
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 4 cut(s) 38, 62, 714, 794
BfoI RGCGCY 1 cut(s) 902
BisI GCNGC 4 cut(s) 768, 894, 897, 902
BlnI CCTAGG 1 cut(s) 793
BlsI GCNGC 4 cut(s) 769, 895, 898, 903
Bme1390I CCNGG 5 cut(s) 211, 236, 245, 695, 843
Bme18I GGWCC 2 cut(s) 353, 743
BmeT110I CYCGRG 2 cut(s) 636, 872
BmgT120I GGNCC 2 cut(s) 353, 743
BmiI GGNNCC 4 cut(s) 634, 745, 799, 869
BmrFI CCNGG 5 cut(s) 211, 236, 245, 695, 843
BmsI GCATC 1 cut(s) 1184
BoxI GACNNNNGTC 1 cut(s) 1184
BpmI CTGGAG 1 cut(s) 864
BpuMI CCSGG 4 cut(s) 211, 236, 245, 695
BsaJI CCNNGG 7 cut(s) 243, 529, 793, 841, 871, 1021, 1110
BsaWI WCCGGW 3 cut(s) 782, 948, 1181
BsaXI ACNNNNNCTCC 1 cut(s) 1195
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 697
Bse118I RCCGGY 2 cut(s) 782, 1181
Bse1I ACTGG 3 cut(s) 262, 915, 962
BseAI TCCGGA 1 cut(s) 948
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 7 cut(s) 243, 529, 793, 841, 871, 1021, 1110
BseGI GGATG 5 cut(s) 154, 227, 306, 550, 759
BseLI CCNNNNNNNGG 2 cut(s) 296, 697
BseNI ACTGG 3 cut(s) 262, 915, 962
BseRI GAGGAG 5 cut(s) 388, 469, 565, 680, 1169
BseXI GCAGC 3 cut(s) 779, 888, 905
BsgI GTGCAG 1 cut(s) 58
Bsh1236I CGCG 3 cut(s) 143, 671, 989
BshFI GGCC 3 cut(s) 428, 831, 1109
BshTI ACCGGT 2 cut(s) 782, 1181
BsiHKAI GWGCWC 1 cut(s) 828
BsiHKCI CYCGRG 2 cut(s) 636, 872
BsiSI CCGG 7 cut(s) 210, 236, 245, 695, 783, 949, 1182
BsiWI CGTACG 1 cut(s) 1053
BslI CCNNNNNNNGG 2 cut(s) 296, 697
BsmAI GTCTC 1 cut(s) 392
BsmBI CGTCTC 1 cut(s) 392
BsnI GGCC 3 cut(s) 428, 831, 1109
BsoBI CYCGRG 2 cut(s) 636, 872
Bsp1286I GDGCHC 1 cut(s) 828
Bsp13I TCCGGA 1 cut(s) 948
Bsp143I GATC 3 cut(s) 286, 495, 568
Bsp19I CCATGG 1 cut(s) 1110
BspACI CCGC 5 cut(s) 371, 630, 698, 724, 896
BspANI GGCC 3 cut(s) 428, 831, 1109
BspEI TCCGGA 1 cut(s) 948
BspFNI CGCG 3 cut(s) 143, 671, 989
BspLI GGNNCC 4 cut(s) 634, 745, 799, 869
BspPI GGATC 2 cut(s) 281, 490
BsrFI RCCGGY 2 cut(s) 782, 1181
BsrI ACTGG 3 cut(s) 262, 915, 962
BssAI RCCGGY 2 cut(s) 782, 1181
BssECI CCNNGG 7 cut(s) 243, 529, 793, 841, 871, 1021, 1110
BssMI GATC 3 cut(s) 286, 495, 568
BssSI CACGAG 2 cut(s) 264, 1123
BssT1I CCWWGG 4 cut(s) 529, 793, 1021, 1110
Bst2BI CACGAG 2 cut(s) 264, 1123
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 3 cut(s) 416, 616, 1073
Bst6I CTCTTC 4 cut(s) 123, 337, 397, 1160
BstDEI CTNAG 1 cut(s) 492
BstDSI CCRYGG 1 cut(s) 1110
BstF5I GGATG 5 cut(s) 154, 227, 306, 550, 759
BstFNI CGCG 3 cut(s) 143, 671, 989
BstH2I RGCGCY 1 cut(s) 902
BstHHI GCGC 2 cut(s) 250, 901
BstKTI GATC 3 cut(s) 289, 498, 571
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 3 cut(s) 286, 495, 568
BstMWI GCNNNNNNNGC 2 cut(s) 140, 710
BstNI CCWGG 1 cut(s) 843
BstPAI GACNNNNGTC 1 cut(s) 1184
BstSCI CCNGG 5 cut(s) 209, 234, 243, 693, 841
BstUI CGCG 3 cut(s) 143, 671, 989
BstV1I GCAGC 3 cut(s) 779, 888, 905
BstX2I RGATCY 2 cut(s) 286, 495
BstYI RGATCY 2 cut(s) 286, 495
BsuRI GGCC 3 cut(s) 428, 831, 1109
BtgI CCRYGG 1 cut(s) 1110
BtgZI GCGATG 1 cut(s) 259
BtsCI GGATG 5 cut(s) 154, 227, 306, 550, 759
BtsI GCAGTG 2 cut(s) 70, 1204
BtsIMutI CAGTG 3 cut(s) 70, 908, 1204
CfoI GCGC 2 cut(s) 250, 901
Cfr10I RCCGGY 2 cut(s) 782, 1181
Cfr13I GGNCC 2 cut(s) 353, 743
CseI GACGC 2 cut(s) 123, 1096
Csp6I GTAC 3 cut(s) 955, 985, 1054
CspAI ACCGGT 2 cut(s) 782, 1181
CspCI CAANNNNNGTGG 2 cut(s) 488, 523
CviAII CATG 2 cut(s) 503, 1111
CviQI GTAC 3 cut(s) 955, 985, 1054
DdeI CTNAG 1 cut(s) 492
DpnI GATC 3 cut(s) 288, 497, 570
DpnII GATC 3 cut(s) 286, 495, 568
DraI TTTAAA 1 cut(s) 645
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 3 cut(s) 426, 829, 1107
Eam1104I CTCTTC 4 cut(s) 123, 337, 397, 1160
EarI CTCTTC 4 cut(s) 123, 337, 397, 1160
EciI GGCGGA 1 cut(s) 713
Eco130I CCWWGG 4 cut(s) 529, 793, 1021, 1110
Eco32I GATATC 1 cut(s) 675
Eco47I GGWCC 2 cut(s) 353, 743
Eco57I CTGAAG 3 cut(s) 318, 329, 585
Eco88I CYCGRG 2 cut(s) 636, 872
EcoO109I RGGNCCY 2 cut(s) 353, 743
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 841
EcoRV GATATC 1 cut(s) 675
EcoT14I CCWWGG 4 cut(s) 529, 793, 1021, 1110
ErhI CCWWGG 4 cut(s) 529, 793, 1021, 1110
Esp3I CGTCTC 1 cut(s) 392
FaeI CATG 2 cut(s) 506, 1114
FaiI YATR 9 cut(s) 151, 462, 504, 525, 834, 855, 1078, 1112, 1209
FatI CATG 2 cut(s) 502, 1110
FauI CCCGC 1 cut(s) 691
FblI GTMKAC 1 cut(s) 1085
Fnu4HI GCNGC 4 cut(s) 768, 894, 897, 902
FokI GGATG 5 cut(s) 141, 234, 293, 537, 746
Fsp4HI GCNGC 4 cut(s) 768, 894, 897, 902
FspBI CTAG 4 cut(s) 38, 62, 714, 794
GlaI GCGC 2 cut(s) 249, 900
GluI GCNGC 4 cut(s) 768, 894, 897, 902
GsuI CTGGAG 1 cut(s) 864
HaeII RGCGCY 1 cut(s) 902
HaeIII GGCC 3 cut(s) 428, 831, 1109
HapII CCGG 7 cut(s) 210, 236, 245, 695, 783, 949, 1182
HgaI GACGC 2 cut(s) 123, 1096
HhaI GCGC 2 cut(s) 250, 901
Hin1II CATG 2 cut(s) 506, 1114
Hin6I GCGC 2 cut(s) 248, 899
HinP1I GCGC 2 cut(s) 248, 899
HincII GTYRAC 3 cut(s) 667, 1086, 1120
HindII GTYRAC 3 cut(s) 667, 1086, 1120
HindIII AAGCTT 3 cut(s) 47, 97, 341
HinfI GANTC 7 cut(s) 13, 56, 239, 329, 652, 819, 1204
HpaI GTTAAC 1 cut(s) 1120
HpaII CCGG 7 cut(s) 210, 236, 245, 695, 783, 949, 1182
HphI GGTGA 3 cut(s) 195, 1009, 1021
Hpy166II GTNNAC 4 cut(s) 147, 667, 1086, 1120
Hpy188I TCNGA 5 cut(s) 291, 348, 456, 867, 926
Hpy188III TCNNGA 9 cut(s) 53, 103, 173, 284, 326, 603, 636, 874, 949
Hpy8I GTNNAC 4 cut(s) 147, 667, 1086, 1120
Hpy99I CGWCG 3 cut(s) 88, 94, 1090
HpyAV CCTTC 5 cut(s) 216, 323, 589, 1037, 1041
HpyCH4III ACNGT 3 cut(s) 416, 616, 1073
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 5 cut(s) 75, 611, 733, 904, 1197
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 710
HpyF3I CTNAG 1 cut(s) 492
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 2 cut(s) 506, 1114
HspAI GCGC 2 cut(s) 248, 899
Kpn2I TCCGGA 1 cut(s) 948
KspAI GTTAAC 1 cut(s) 1120
Kzo9I GATC 3 cut(s) 286, 495, 568
LmnI GCTCC 2 cut(s) 701, 867
Lsp1109I GCAGC 3 cut(s) 779, 888, 905
LweI GCATC 1 cut(s) 1184
MaeI CTAG 4 cut(s) 38, 62, 714, 794
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 8 cut(s) 68, 533, 595, 649, 706, 847, 858, 1067
MalI GATC 3 cut(s) 288, 497, 570
MboI GATC 3 cut(s) 286, 495, 568
MboII GAAGA 7 cut(s) 23, 26, 110, 324, 384, 1171, 1177
MflI RGATCY 2 cut(s) 286, 495
MhlI GDGCHC 1 cut(s) 828
MlsI TGGCCA 2 cut(s) 428, 1109
MluCI AATT 2 cut(s) 292, 835
MluI ACGCGT 1 cut(s) 987
MluNI TGGCCA 2 cut(s) 428, 1109
MlyI GAGTC 3 cut(s) 323, 646, 1213
MmeI TCCRAC 2 cut(s) 146, 199
Mox20I TGGCCA 2 cut(s) 428, 1109
MroI TCCGGA 1 cut(s) 948
MroXI GAANNNNTTC 2 cut(s) 98, 1016
MscI TGGCCA 2 cut(s) 428, 1109
MseI TTAA 6 cut(s) 231, 471, 618, 644, 1037, 1119
Msp20I TGGCCA 2 cut(s) 428, 1109
MspA1I CMGCKG 2 cut(s) 630, 896
MspI CCGG 7 cut(s) 210, 236, 245, 695, 783, 949, 1182
MspR9I CCNGG 5 cut(s) 211, 236, 245, 695, 843
MvaI CCWGG 1 cut(s) 843
MvnI CGCG 3 cut(s) 143, 671, 989
MwoI GCNNNNNNNGC 2 cut(s) 140, 710
NciI CCSGG 4 cut(s) 211, 236, 245, 695
NcoI CCATGG 1 cut(s) 1110
NdeII GATC 3 cut(s) 286, 495, 568
NlaIII CATG 2 cut(s) 506, 1114
NlaIV GGNNCC 4 cut(s) 634, 745, 799, 869
NmeAIII GCCGAG 1 cut(s) 807
NmuCI GTSAC 4 cut(s) 68, 595, 649, 847
PcsI WCGNNNNNNNCGW 2 cut(s) 89, 171
PdmI GAANNNNTTC 2 cut(s) 98, 1016
PfeI GAWTC 4 cut(s) 13, 56, 239, 819
Pfl23II CGTACG 1 cut(s) 1053
PflFI GACNNNGTC 1 cut(s) 380
PinAI ACCGGT 2 cut(s) 782, 1181
PkrI GCNGC 4 cut(s) 769, 895, 898, 903
PleI GAGTC 3 cut(s) 323, 646, 1212
PpsI GAGTC 3 cut(s) 323, 646, 1212
PpuMI RGGWCCY 2 cut(s) 353, 743
PshAI GACNNNNGTC 1 cut(s) 1184
Psp5II RGGWCCY 2 cut(s) 353, 743
Psp6I CCWGG 1 cut(s) 841
PspGI CCWGG 1 cut(s) 841
PspLI CGTACG 1 cut(s) 1053
PspN4I GGNNCC 4 cut(s) 634, 745, 799, 869
PspPI GGNCC 2 cut(s) 353, 743
PspPPI RGGWCCY 2 cut(s) 353, 743
PsuI RGATCY 2 cut(s) 286, 495
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 3 cut(s) 956, 986, 1055
RsaNI GTAC 3 cut(s) 955, 985, 1054
SalI GTCGAC 1 cut(s) 1084
SaqAI TTAA 6 cut(s) 231, 471, 618, 644, 1037, 1119
SatI GCNGC 4 cut(s) 768, 894, 897, 902
Sau3AI GATC 3 cut(s) 286, 495, 568
Sau96I GGNCC 2 cut(s) 353, 743
SchI GAGTC 3 cut(s) 323, 646, 1213
ScrFI CCNGG 5 cut(s) 211, 236, 245, 695, 843
SduI GDGCHC 1 cut(s) 828
SfaNI GCATC 1 cut(s) 1184
SinI GGWCC 2 cut(s) 353, 743
Sse9I AATT 2 cut(s) 292, 835
SsiI CCGC 5 cut(s) 371, 630, 698, 724, 896
SspMI CTAG 4 cut(s) 38, 62, 714, 794
StyD4I CCNGG 5 cut(s) 209, 234, 243, 693, 841
StyI CCWWGG 4 cut(s) 529, 793, 1021, 1110
TaaI ACNGT 3 cut(s) 416, 616, 1073
TaiI ACGT 1 cut(s) 324
TaqI TCGA 6 cut(s) 102, 174, 482, 1085, 1141, 1188
TasI AATT 2 cut(s) 292, 835
TauI GCSGC 1 cut(s) 899
TfiI GAWTC 4 cut(s) 13, 56, 239, 819
Tru1I TTAA 6 cut(s) 231, 471, 618, 644, 1037, 1119
Tru9I TTAA 6 cut(s) 231, 471, 618, 644, 1037, 1119
TscAI CASTG 3 cut(s) 77, 915, 1204
TseFI GTSAC 4 cut(s) 68, 595, 649, 847
TseI GCWGC 3 cut(s) 767, 893, 901
Tsp45I GTSAC 4 cut(s) 68, 595, 649, 847
TspDTI ATGAA 3 cut(s) 187, 539, 1178
TspGWI ACGGA 4 cut(s) 374, 425, 439, 672
TspRI CASTG 3 cut(s) 77, 915, 1204
Tth111I GACNNNGTC 1 cut(s) 380
VpaK11BI GGWCC 2 cut(s) 353, 743
XapI RAATTY 1 cut(s) 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 839
XmaJI CCTAGG 1 cut(s) 793
XmiI GTMKAC 1 cut(s) 1085
XmnI GAANNNNTTC 2 cut(s) 98, 1016
XspI CTAG 4 cut(s) 38, 62, 714, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.