MD17G1111800.v1.1

Protein of unknown function (DUF568)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012003
Physical Location & Seq
Forward (+)
9586391 .. 9587590
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1111800.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGCTCTTCTTCTTATCCCTCTTCCTTCTCTTTTCCTTTCCCCATGTCACATTTTCTGCCCATTGCACCACCACCGCCGCCGCCAAAACCTTTCAAAAATGCATCACACTCCCTACCCAACAAGCCTCCATAGCATGGACTTTCCATCGCCACAATGCCACCCTAGACCTTGTTTTCTTTGGTACTTTCATATCTCCTTCTGGGTGGGTTGGGTGGGGCATCAACCCGACCTCCCCGGAAATGACTGGCACACGTGCTCTAATTGCCTTTCCGGACCCTAGCACCGGTCAAATAGTTCTCCTCACTTACCTCTTAGACCCAACCACAAAGCTCCAAAAACGCCCTCTCCTCTCTCGCCCTCTTGACATCCACATTCTCTCCTCGTCCGCCACCCTATACGGCGGGAAATTAGCCACCGTCCACGGCGGTGCTGCAGTCCAAATCTTCGCTACATTAAAACTCGCGCCGAACAACACAAAGCTCCATCACGTGTGGAACCGCGGCCTCTACGTCCAAGGCTACTCGCCAACCATACACCCAACCACAGCCAACGACCTTTCATCCATTACGACATTCGATGTCATGTCAGGCACAACGGCCACACGTCACTCCAACATTGGCACACTCAGAACAGTGCATGGCATCATGAACGCCATTGCATGGGGCATCATGCTCCCCATTGGAGCAGTGCTTGCACGCTACCTCAGGCACGTACAATCATTAGGGCCCACATGGTTCTATGTGCACGCTGGGGTCCAACTGTTTGCTTTTTTCCTAGGGACTGTAGGGTTTGCTATTGGTATTAGGCTTGGGGATATGTCACCTGGGGTCCAGTATGGGCTCCACAGGAAGCTAGGGTTTGCAGCATTTTGCTTGGGAGGGTTGCAAACCCTAGCACTATTGTTTAGACCTAAAACTACAACCAAGTTCAGGAAGTACTGGAAATCTTACCACCATTTTGTTGGGTATGCATGTGTGGTGCTGGGTGTGGTGAATGTTTTCCAAGGTTTTGAGGTGATGGGAGAAGGAAGGTCTATTGCTAAACTGGTTTACTGTTTAGGATTGTCCACACTGATTGGGGTTTGTATTACTTTGGAGGTGAATTCTTGGGTGGTTTTTTGTAGAAAACCAAAGGAGGAGAAGCTGAGGAGGGAAGGTTTAGTTGGAGCGTCTGATAAAGGCAGTGGAATCCTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000293 GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0016723 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

400

Amino Acids

43.67

Weight (kDa)

9.86

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 81 - 193 1.5e-21 Protein of unknown function (DUF568)
Cytochrom_B561 PF03188 240 - 337 2e-07 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04850
fragaria_vesca FvH4_6g41290
malus_domestica MD09G1120800.v1.1 MD17G1111800.v1.1
prunus_persica Prupe.3G202700_v2.0.a1
pyrus_communis pycom09g04540 pycom17g10370
rosa_chinensis RchiOBHm_Chr2g0156851
rosa_laevigata RLG00000020946
rosa_multiflora Rmu_co8482377.1_g000001 Rmu_sc0008149.1_g000001 Rmu_ssc0000286.1_g000024
rosa_roxburghii Rroxscaffold_2G00092670
rosa_rugosa Rorug02G0456600
rosa_samantha Rh2AG522200 Rh2BG535600 Rh2CG507100 Rh2DG544600
rosa_wichuraiana Rw2G043120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 135, 660
AccII CGCG 2 cut(s) 464, 501
AccIII TCCGGA 1 cut(s) 271
AciI CCGC 8 cut(s) 75, 78, 81, 387, 402, 426, 499, 501
AcoI YGGCCR 1 cut(s) 597
AcsI RAATTY 1 cut(s) 1102
AcvI CACGTG 2 cut(s) 254, 490
AfaI GTAC 3 cut(s) 184, 714, 938
AfiI CCNNNNNNNGG 4 cut(s) 135, 284, 660, 930
AflIII ACRYGT 3 cut(s) 251, 489, 602
AgeI ACCGGT 1 cut(s) 284
AgsI TTSAA 1 cut(s) 95
AjiI CACGTC 1 cut(s) 605
AjnI CCWGG 1 cut(s) 823
AjuI GAANNNNNNNTTGG 2 cut(s) 1146, 1178
AleI CACNNNNGTG 1 cut(s) 426
AluBI AGCT 4 cut(s) 331, 481, 853, 1144
AluI AGCT 4 cut(s) 331, 481, 853, 1144
Alw21I GWGCWC 2 cut(s) 259, 747
Alw44I GTGCAC 1 cut(s) 743
Aor13HI TCCGGA 1 cut(s) 271
AoxI GGCC 3 cut(s) 502, 597, 725
ApaI GGGCCC 1 cut(s) 729
ApaLI GTGCAC 1 cut(s) 743
ApeKI GCWGC 2 cut(s) 431, 863
ApoI RAATTY 1 cut(s) 1102
AsiGI ACCGGT 1 cut(s) 284
Asp700I GAANNNNTTC 1 cut(s) 998
AspA2I CCTAGG 1 cut(s) 775
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 5 cut(s) 274, 725, 726, 754, 829
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 4 cut(s) 813, 1003, 1027, 1111
AvaII GGWCC 3 cut(s) 274, 754, 829
AvrII CCTAGG 1 cut(s) 775
AxyI CCTNAGG 1 cut(s) 704
BaeGI GKGCMC 2 cut(s) 729, 747
BanII GRGCYC 2 cut(s) 729, 843
BbrPI CACGTG 2 cut(s) 254, 490
Bbv12I GWGCWC 2 cut(s) 259, 747
BbvCI CCTCAGC 1 cut(s) 1145
BbvI GCAGC 2 cut(s) 418, 875
BccI CCATC 3 cut(s) 153, 492, 1012
BceAI ACGGC 3 cut(s) 415, 439, 612
BciT130I CCWGG 1 cut(s) 825
BcnI CCSGG 1 cut(s) 236
BfaI CTAG 5 cut(s) 164, 279, 776, 854, 893
BfmI CTRYAG 2 cut(s) 432, 783
BisI GCNGC 5 cut(s) 78, 81, 432, 502, 864
BlnI CCTAGG 1 cut(s) 775
BlsI GCNGC 5 cut(s) 79, 82, 433, 503, 865
BmcAI AGTACT 1 cut(s) 938
Bme1390I CCNGG 2 cut(s) 236, 825
Bme18I GGWCC 3 cut(s) 274, 754, 829
BmgBI CACGTC 1 cut(s) 605
BmgT120I GGNCC 5 cut(s) 274, 725, 726, 754, 829
BmiI GGNNCC 6 cut(s) 276, 497, 727, 755, 830, 842
BmrFI CCNGG 2 cut(s) 236, 825
BmsI GCATC 4 cut(s) 111, 228, 651, 675
Bpu10I CCTNAGC 1 cut(s) 1145
BpuMI CCSGG 1 cut(s) 236
BsaAI YACGTR 3 cut(s) 254, 490, 712
BsaJI CCNNGG 7 cut(s) 234, 421, 499, 514, 775, 824, 1003
BsaWI WCCGGW 2 cut(s) 271, 284
BsaXI ACNNNNNCTCC 8 cut(s) 215, 245, 330, 360, 362, 392, 1141, 1171
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 284, 660, 930
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 4 cut(s) 250, 832, 944, 1050
Bse21I CCTNAGG 1 cut(s) 704
Bse3DI GCAATG 2 cut(s) 61, 654
BseAI TCCGGA 1 cut(s) 271
BseBI CCWGG 1 cut(s) 825
BseDI CCNNGG 7 cut(s) 234, 421, 499, 514, 775, 824, 1003
BseGI GGATG 2 cut(s) 366, 560
BseLI CCNNNNNNNGG 4 cut(s) 135, 284, 660, 930
BseMI GCAATG 2 cut(s) 61, 654
BseMII CTCAG 3 cut(s) 640, 718, 1136
BseNI ACTGG 4 cut(s) 250, 832, 944, 1050
BseRI GAGGAG 5 cut(s) 290, 338, 370, 1151, 1162
BseSI GKGCMC 2 cut(s) 729, 747
BseXI GCAGC 2 cut(s) 418, 875
BseYI CCCAGC 2 cut(s) 749, 982
Bsh1236I CGCG 2 cut(s) 464, 501
BshFI GGCC 3 cut(s) 504, 599, 727
BshTI ACCGGT 1 cut(s) 284
BsiHKAI GWGCWC 2 cut(s) 259, 747
BsiSI CCGG 3 cut(s) 236, 272, 285
BslFI GGGAC 1 cut(s) 793
BslI CCNNNNNNNGG 4 cut(s) 135, 284, 660, 930
BsmFI GGGAC 1 cut(s) 793
BsnI GGCC 3 cut(s) 504, 599, 727
Bsp120I GGGCCC 1 cut(s) 725
Bsp1286I GDGCHC 4 cut(s) 259, 729, 747, 843
Bsp13I TCCGGA 1 cut(s) 271
BspACI CCGC 8 cut(s) 75, 78, 81, 387, 402, 426, 499, 501
BspANI GGCC 3 cut(s) 504, 599, 727
BspCNI CTCAG 3 cut(s) 639, 717, 1137
BspEI TCCGGA 1 cut(s) 271
BspFNI CGCG 2 cut(s) 464, 501
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 6 cut(s) 276, 497, 727, 755, 830, 842
BspMAI CTGCAG 1 cut(s) 436
BspQI GCTCTTC 1 cut(s) 11
BsrDI GCAATG 2 cut(s) 61, 654
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 4 cut(s) 250, 832, 944, 1050
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 7 cut(s) 234, 421, 499, 514, 775, 824, 1003
BssT1I CCWWGG 3 cut(s) 514, 775, 1003
Bst2UI CCWGG 1 cut(s) 825
Bst4CI ACNGT 5 cut(s) 418, 634, 762, 784, 1055
Bst6I CTCTTC 2 cut(s) 11, 26
BstAPI GCANNNNNTGC 1 cut(s) 692
BstBAI YACGTR 3 cut(s) 254, 490, 712
BstC8I GCNNGC 3 cut(s) 693, 697, 747
BstDEI CTNAG 5 cut(s) 313, 626, 704, 1145, 1193
BstDSI CCRYGG 2 cut(s) 421, 499
BstF5I GGATG 2 cut(s) 366, 560
BstFNI CGCG 2 cut(s) 464, 501
BstHHI GCGC 1 cut(s) 466
BstMWI GCNNNNNNNGC 3 cut(s) 131, 263, 692
BstNI CCWGG 1 cut(s) 825
BstNSI RCATGY 1 cut(s) 975
BstSCI CCNGG 2 cut(s) 234, 823
BstSFI CTRYAG 2 cut(s) 432, 783
BstSLI GKGCMC 2 cut(s) 729, 747
BstUI CGCG 2 cut(s) 464, 501
BstV1I GCAGC 2 cut(s) 418, 875
BstXI CCANNNNNNTGG 1 cut(s) 962
Bsu36I CCTNAGG 1 cut(s) 704
BsuRI GGCC 3 cut(s) 504, 599, 727
BtgI CCRYGG 2 cut(s) 421, 499
BtgZI GCGATG 1 cut(s) 131
BtrI CACGTC 1 cut(s) 605
BtsCI GGATG 2 cut(s) 366, 560
BtsI GCAGTG 2 cut(s) 693, 1189
BtsIMutI CAGTG 4 cut(s) 639, 693, 1070, 1189
Cac8I GCNNGC 3 cut(s) 693, 697, 747
CciI TCATGA 1 cut(s) 645
CfoI GCGC 1 cut(s) 466
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 5 cut(s) 274, 725, 726, 754, 829
Cfr42I CCGCGG 1 cut(s) 502
CseI GACGC 1 cut(s) 1158
Csp6I GTAC 3 cut(s) 183, 713, 937
CspAI ACCGGT 1 cut(s) 284
CspCI CAANNNNNGTGG 2 cut(s) 1057, 1092
CviAII CATG 9 cut(s) 44, 135, 583, 638, 646, 660, 670, 732, 972
CviQI GTAC 3 cut(s) 183, 713, 937
DdeI CTNAG 5 cut(s) 313, 626, 704, 1145, 1193
EaeI YGGCCR 1 cut(s) 597
Eam1104I CTCTTC 2 cut(s) 11, 26
EarI CTCTTC 2 cut(s) 11, 26
EciI GGCGGA 1 cut(s) 376
Eco130I CCWWGG 3 cut(s) 514, 775, 1003
Eco24I GRGCYC 2 cut(s) 729, 843
Eco47I GGWCC 3 cut(s) 274, 754, 829
Eco72I CACGTG 2 cut(s) 254, 490
Eco81I CCTNAGG 1 cut(s) 704
EcoO109I RGGNCCY 1 cut(s) 725
EcoRI GAATTC 1 cut(s) 1102
EcoRII CCWGG 1 cut(s) 823
EcoT14I CCWWGG 3 cut(s) 514, 775, 1003
EcoT22I ATGCAT 2 cut(s) 104, 973
EcoT38I GRGCYC 2 cut(s) 729, 843
ErhI CCWWGG 3 cut(s) 514, 775, 1003
FaeI CATG 9 cut(s) 47, 138, 586, 641, 649, 663, 673, 735, 975
FaqI GGGAC 1 cut(s) 793
FatI CATG 9 cut(s) 43, 134, 582, 637, 645, 659, 669, 731, 971
FauI CCCGC 1 cut(s) 395
Fnu4HI GCNGC 5 cut(s) 78, 81, 432, 502, 864
FokI GGATG 2 cut(s) 353, 547
FriOI GRGCYC 2 cut(s) 729, 843
Fsp4HI GCNGC 5 cut(s) 78, 81, 432, 502, 864
FspBI CTAG 5 cut(s) 164, 279, 776, 854, 893
GlaI GCGC 1 cut(s) 465
GluI GCNGC 5 cut(s) 78, 81, 432, 502, 864
GsaI CCCAGC 2 cut(s) 753, 986
HaeIII GGCC 3 cut(s) 504, 599, 727
HapII CCGG 3 cut(s) 236, 272, 285
HgaI GACGC 1 cut(s) 1158
HhaI GCGC 1 cut(s) 466
Hin1II CATG 9 cut(s) 47, 138, 586, 641, 649, 663, 673, 735, 975
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HinfI GANTC 1 cut(s) 1188
HpaII CCGG 3 cut(s) 236, 272, 285
HphI GGTGA 4 cut(s) 813, 1003, 1027, 1111
Hpy166II GTNNAC 4 cut(s) 421, 745, 1051, 1068
Hpy188I TCNGA 2 cut(s) 629, 1174
Hpy188III TCNNGA 4 cut(s) 272, 362, 646, 931
Hpy8I GTNNAC 4 cut(s) 421, 745, 1051, 1068
HpyAV CCTTC 5 cut(s) 35, 207, 1019, 1023, 1148
HpyCH4III ACNGT 5 cut(s) 418, 634, 762, 784, 1055
HpyCH4IV ACGT 5 cut(s) 253, 489, 510, 604, 711
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 263, 692
HpyF3I CTNAG 5 cut(s) 313, 626, 704, 1145, 1193
HpySE526I ACGT 5 cut(s) 253, 489, 510, 604, 711
Hsp92II CATG 9 cut(s) 47, 138, 586, 641, 649, 663, 673, 735, 975
HspAI GCGC 1 cut(s) 464
Kpn2I TCCGGA 1 cut(s) 271
KspI CCGCGG 1 cut(s) 502
LguI GCTCTTC 1 cut(s) 11
LmnI GCTCC 6 cut(s) 336, 486, 678, 683, 846, 1166
Lsp1109I GCAGC 2 cut(s) 418, 875
LweI GCATC 4 cut(s) 111, 228, 651, 675
MaeI CTAG 5 cut(s) 164, 279, 776, 854, 893
MaeII ACGT 5 cut(s) 253, 489, 510, 604, 711
MaeIII GTNAC 3 cut(s) 46, 605, 819
MboII GAAGA 2 cut(s) 13, 436
MhlI GDGCHC 4 cut(s) 259, 729, 747, 843
MluCI AATT 3 cut(s) 261, 407, 1102
MmeI TCCRAC 3 cut(s) 636, 781, 1144
Mph1103I ATGCAT 2 cut(s) 104, 973
MroI TCCGGA 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 998
MseI TTAA 1 cut(s) 455
MslI CAYNNNNRTG 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 501
MspI CCGG 3 cut(s) 236, 272, 285
MspR9I CCNGG 2 cut(s) 236, 825
MvaI CCWGG 1 cut(s) 825
MvnI CGCG 2 cut(s) 464, 501
MwoI GCNNNNNNNGC 3 cut(s) 131, 263, 692
NciI CCSGG 1 cut(s) 236
NlaIII CATG 9 cut(s) 47, 138, 586, 641, 649, 663, 673, 735, 975
NlaIV GGNNCC 6 cut(s) 276, 497, 727, 755, 830, 842
NmuCI GTSAC 3 cut(s) 46, 605, 819
NsiI ATGCAT 2 cut(s) 104, 973
NspI RCATGY 1 cut(s) 975
OliI CACNNNNGTG 1 cut(s) 426
PagI TCATGA 1 cut(s) 645
PciSI GCTCTTC 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 998
PfeI GAWTC 1 cut(s) 1188
PflMI CCANNNNNTGG 2 cut(s) 135, 660
PinAI ACCGGT 1 cut(s) 284
PkrI GCNGC 5 cut(s) 79, 82, 433, 503, 865
PmaCI CACGTG 2 cut(s) 254, 490
PmlI CACGTG 2 cut(s) 254, 490
Ppu21I YACGTR 3 cut(s) 254, 490, 712
Psp6I CCWGG 1 cut(s) 823
PspCI CACGTG 2 cut(s) 254, 490
PspFI CCCAGC 2 cut(s) 749, 982
PspGI CCWGG 1 cut(s) 823
PspN4I GGNNCC 6 cut(s) 276, 497, 727, 755, 830, 842
PspOMI GGGCCC 1 cut(s) 725
PspPI GGNCC 5 cut(s) 274, 725, 726, 754, 829
PstI CTGCAG 1 cut(s) 436
RsaI GTAC 3 cut(s) 184, 714, 938
RsaNI GTAC 3 cut(s) 183, 713, 937
RseI CAYNNNNRTG 1 cut(s) 426
SacII CCGCGG 1 cut(s) 502
SapI GCTCTTC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 455
SatI GCNGC 5 cut(s) 78, 81, 432, 502, 864
Sau96I GGNCC 5 cut(s) 274, 725, 726, 754, 829
ScaI AGTACT 1 cut(s) 938
ScrFI CCNGG 2 cut(s) 236, 825
SduI GDGCHC 4 cut(s) 259, 729, 747, 843
SfaNI GCATC 4 cut(s) 111, 228, 651, 675
SfcI CTRYAG 2 cut(s) 432, 783
Sfr303I CCGCGG 1 cut(s) 502
SgrBI CCGCGG 1 cut(s) 502
SinI GGWCC 3 cut(s) 274, 754, 829
SmiMI CAYNNNNRTG 1 cut(s) 426
Sse9I AATT 3 cut(s) 261, 407, 1102
SsiI CCGC 8 cut(s) 75, 78, 81, 387, 402, 426, 499, 501
SspMI CTAG 5 cut(s) 164, 279, 776, 854, 893
StyD4I CCNGG 2 cut(s) 234, 823
StyI CCWWGG 3 cut(s) 514, 775, 1003
TaaI ACNGT 5 cut(s) 418, 634, 762, 784, 1055
TaiI ACGT 5 cut(s) 256, 492, 513, 607, 714
TaqI TCGA 1 cut(s) 576
TasI AATT 3 cut(s) 261, 407, 1102
TatI WGTACW 1 cut(s) 936
TauI GCSGC 3 cut(s) 80, 83, 504
TfiI GAWTC 1 cut(s) 1188
Tru1I TTAA 1 cut(s) 455
Tru9I TTAA 1 cut(s) 455
TscAI CASTG 4 cut(s) 639, 693, 1077, 1189
TseFI GTSAC 3 cut(s) 46, 605, 819
TseI GCWGC 2 cut(s) 431, 863
Tsp45I GTSAC 3 cut(s) 46, 605, 819
TspDTI ATGAA 3 cut(s) 178, 549, 662
TspRI CASTG 4 cut(s) 639, 693, 1077, 1189
Van91I CCANNNNNTGG 2 cut(s) 135, 660
VneI GTGCAC 1 cut(s) 743
VpaK11BI GGWCC 3 cut(s) 274, 754, 829
XapI RAATTY 1 cut(s) 1102
XceI RCATGY 1 cut(s) 975
XcmI CCANNNNNNNNNTGG 1 cut(s) 959
XmaJI CCTAGG 1 cut(s) 775
XmnI GAANNNNTTC 1 cut(s) 998
XspI CTAG 5 cut(s) 164, 279, 776, 854, 893
ZrmI AGTACT 1 cut(s) 938
Zsp2I ATGCAT 2 cut(s) 104, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.