AT2G18730

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011984
Physical Location & Seq
Forward (+)
8118736 .. 8121947
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G18730.1

Sequence Viewer

Length: 1467 bp
ATGGACTCTCCGGTATCAAAAACCGATGCATCGAAAGAGAAATTCGTGGCTTCACGACCATCAACGGCTGATTCAAAGACGATGAGAGGTTGTGGACTCGCGAATCTGGCATGGGTTGGTGTAGATAAAGTAGAGCTTCGTCAGAGGTTGATGATGCCTGAGTATCTTCGTCTCGCTATGCGTGACTGTATCAAACGCAAAGATTCCTCGGCTATTCCTGATCATTTACTTCTTCCTGGTGGTGCTGTTGCTGATATGGCTCCTCATGCTCCTATGGTTGTTTTCATTAACCCCAATTCTGGTGGTCGTCATGGTCCTGTTCTTAAAGAGAGGCTTCAACAGTTGATGAGCGAGGAACAGGTGTTTGACCTCACGGAAGTGAAGCCGCATGAGTTTGTTCGATACGGGTTGGGGTGTTTGGAGAAAGTGGCAGCGGAAGGAGATGAATGTGCAAAAGAATGCAGAGCAAGGTTGAGGATTATGGTTGCAGGAGGTGATGGTACTGTTGGTTGGGTTCTTGGATGTCTTGGAGAGCTTAATAAAGATGGAAAGTCGCAAATTCCTCCTGTTGGAGTTATTCCTCTCGGTACAGGGAATGATCTTTCAAGGAGTTTTGGTTGGGGTGGTTCGTTTCCTTTTGCGTGGAGATCTGCTGTTAAAAGAACACTCCATCGCGCAAGTATGGGTCCAGTTGCTAGACTTGACAGCTGGAAGATTTTAGTGTCAATGCCATCTGGAGAAGTCGTAGATCCTCCTTATTCTTTAAAACCCGCTGAAGAAAACGAACTTGATCAGGGTTTGGACGCGGGAATTGATGCGCCTCCACTTGCAAAGGCTTATGAAGGTGTTTTCTACAATTACTTAAGCATAGGTATGGATGCTCAAGTTGCATATGGCTTCCACCATCTTCGCAACACTAAACCATATCTTGCTCAAGGCCCTATTTCAAATAAGATTATATATTCAAGTTTTGGTTGCAGTCAGGGTTGGTTTTGCACGCCTTGTGTCAACGATCCAGGTTTAAGGGGACTTAGGAACATAATGAAAATTCACATCAAAAAGGTTAACTGCTCTCAGTGGGAAGAGATCGCAGTCCCGAAAAATGTGAGATCAATTGTCGCATTGAATCTTCACAGCTATGGAAGTGGAAGCCATCCTTGGGGCAATCTAAAACCGGACTATCTAGAGAAGAGAGGCTTTGTGGAAGCACATTGTGATGATGGTTTGATAGAGATTTTCGGTTTTAAGCAAGGATGGCACGCGTCATTTGTCATGGCTGAGCTCATCTCTGCCAAGCACATAGCTCAGGCTGCTGCGGTAAGATTTGAGCTAAGAGGAGGTGACTGGAGAGACGCATTCTTGCAAATGGATGGTGAGCCATGGAAGCAACCAATGAGCACTGAGTACTCAACATTTGTGGAGATCAAGAAGGTTCCTTATCAATCTCTGATGATAAATAATGAATGA

Protein Analysis

488

Amino Acids

53.88

Weight (kDa)

7.58

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 92 - 230 9.4e-26 Diacylglycerol kinase catalytic domain
DAGK_acc PF00609 283 - 460 1.1e-41 Diacylglycerol kinase accessory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18730 AT4G30340 AT4G30340 AT5G57690
fragaria_vesca FvH4_4g08400 FvH4_4g08400
malus_domestica MD13G1262800.v1.1 MD16G1264500.v1.1
prunus_persica Prupe.1G094900_v2.0.a1
pyrus_communis pycom13g24340 pycom16g23310
rosa_chinensis RchiOBHm_Chr4g0404101
rosa_laevigata RLG00000008925
rosa_multiflora Rmu_sc0006579.1_g000001
rosa_roxburghii Rroxscaffold_5G00348640
rosa_rugosa Rorug04G0046900 Rorug04G0047000
rosa_samantha Rh4DG115900
rosa_wichuraiana Rw4G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 101, 675, 806, 1262
AciI CCGC 5 cut(s) 386, 434, 771, 806, 1316
AclWI GGATC 2 cut(s) 743, 1007
AcsI RAATTY 3 cut(s) 41, 558, 1047
AcuI CTGAAG 1 cut(s) 795
AdeI CACNNNGTG 1 cut(s) 1214
AfaI GTAC 3 cut(s) 502, 589, 1406
AfiI CCNNNNNNNGG 3 cut(s) 299, 569, 1159
AflII CTTAAG 1 cut(s) 862
AflIII ACRYGT 1 cut(s) 1260
AgsI TTSAA 6 cut(s) 75, 338, 606, 948, 966, 1126
AjnI CCWGG 2 cut(s) 235, 1015
AjuI GAANNNNNNNTTGG 2 cut(s) 1141, 1173
AleI CACNNNNGTG 1 cut(s) 377
AluBI AGCT 7 cut(s) 136, 535, 708, 1137, 1282, 1304, 1330
AluI AGCT 7 cut(s) 136, 535, 708, 1137, 1282, 1304, 1330
Alw21I GWGCWC 2 cut(s) 1284, 1400
Alw26I GTCTC 2 cut(s) 176, 1344
AlwI GGATC 2 cut(s) 743, 1007
AoxI GGCC 1 cut(s) 937
ApeKI GCWGC 3 cut(s) 431, 1310, 1313
ApoI RAATTY 3 cut(s) 41, 558, 1047
AspLEI GCGC 2 cut(s) 677, 820
AspS9I GGNCC 3 cut(s) 314, 686, 938
AsuHPI GGTGA 3 cut(s) 506, 1352, 1385
AvaII GGWCC 2 cut(s) 314, 686
BanII GRGCYC 1 cut(s) 1284
Bbv12I GWGCWC 2 cut(s) 1284, 1400
BbvI GCAGC 3 cut(s) 443, 1297, 1300
BceAI ACGGC 1 cut(s) 81
BciT130I CCWGG 2 cut(s) 237, 1017
BclI TGATCA 2 cut(s) 220, 790
BcoDI GTCTC 2 cut(s) 176, 1344
BfaI CTAG 2 cut(s) 696, 1184
BfrI CTTAAG 1 cut(s) 862
BglII AGATCT 1 cut(s) 647
BisI GCNGC 4 cut(s) 386, 432, 1311, 1314
BlpI GCTNAGC 1 cut(s) 1278
BlsI GCNGC 4 cut(s) 387, 433, 1312, 1315
BmcAI AGTACT 1 cut(s) 1406
Bme1390I CCNGG 2 cut(s) 237, 1017
Bme18I GGWCC 2 cut(s) 314, 686
BmgT120I GGNCC 3 cut(s) 314, 686, 938
BmiI GGNNCC 3 cut(s) 261, 687, 1434
BmrFI CCNGG 2 cut(s) 237, 1017
BmsI GCATC 5 cut(s) 16, 38, 144, 805, 868
BplI GAGNNNNNCTC 2 cut(s) 1271, 1303
BpmI CTGGAG 2 cut(s) 756, 1366
Bpu10I CCTNAGC 1 cut(s) 1305
Bpu1102I GCTNAGC 1 cut(s) 1278
BpuEI CTTGAG 2 cut(s) 867, 918
BsaJI CCNNGG 3 cut(s) 207, 1157, 1379
BsaWI WCCGGW 2 cut(s) 10, 1174
BsaXI ACNNNNNCTCC 4 cut(s) 601, 631, 729, 759
Bsc4I CCNNNNNNNGG 3 cut(s) 299, 569, 1159
Bse1I ACTGG 2 cut(s) 689, 1349
BseBI CCWGG 2 cut(s) 237, 1017
BseDI CCNNGG 3 cut(s) 207, 1157, 1379
BseGI GGATG 5 cut(s) 527, 883, 1153, 1259, 1375
BseLI CCNNNNNNNGG 3 cut(s) 299, 569, 1159
BseMII CTCAG 5 cut(s) 150, 1088, 1269, 1319, 1392
BseNI ACTGG 2 cut(s) 689, 1349
BseRI GAGGAG 2 cut(s) 252, 1350
BseXI GCAGC 3 cut(s) 443, 1297, 1300
Bsh1236I CGCG 4 cut(s) 101, 675, 806, 1262
BshFI GGCC 1 cut(s) 939
BsiHKAI GWGCWC 2 cut(s) 1284, 1400
BsiSI CCGG 2 cut(s) 11, 1175
BslFI GGGAC 2 cut(s) 1041, 1079
BslI CCNNNNNNNGG 3 cut(s) 299, 569, 1159
BsmAI GTCTC 2 cut(s) 176, 1344
BsmBI CGTCTC 2 cut(s) 176, 1344
BsmFI GGGAC 2 cut(s) 1041, 1079
BsmI GAATGC 2 cut(s) 464, 1355
BsnI GGCC 1 cut(s) 939
Bsp1286I GDGCHC 2 cut(s) 1284, 1400
Bsp143I GATC 9 cut(s) 220, 598, 647, 748, 790, 1012, 1086, 1109, 1422
Bsp1720I GCTNAGC 1 cut(s) 1278
Bsp19I CCATGG 1 cut(s) 1379
Bsp68I TCGCGA 1 cut(s) 101
BspACI CCGC 5 cut(s) 386, 434, 771, 806, 1316
BspANI GGCC 1 cut(s) 939
BspCNI CTCAG 5 cut(s) 151, 1087, 1270, 1318, 1393
BspFNI CGCG 4 cut(s) 101, 675, 806, 1262
BspLI GGNNCC 3 cut(s) 261, 687, 1434
BspPI GGATC 2 cut(s) 743, 1007
BspTI CTTAAG 1 cut(s) 862
BsrI ACTGG 2 cut(s) 689, 1349
BssECI CCNNGG 3 cut(s) 207, 1157, 1379
BssMI GATC 9 cut(s) 220, 598, 647, 748, 790, 1012, 1086, 1109, 1422
BssT1I CCWWGG 2 cut(s) 1157, 1379
Bst2UI CCWGG 2 cut(s) 237, 1017
Bst4CI ACNGT 3 cut(s) 188, 342, 505
Bst6I CTCTTC 2 cut(s) 1077, 1184
BstAFI CTTAAG 1 cut(s) 862
BstC8I GCNNGC 2 cut(s) 998, 1260
BstDEI CTNAG 7 cut(s) 159, 1031, 1074, 1278, 1305, 1331, 1401
BstDSI CCRYGG 1 cut(s) 1379
BstF5I GGATG 5 cut(s) 527, 883, 1153, 1259, 1375
BstFNI CGCG 4 cut(s) 101, 675, 806, 1262
BstHHI GCGC 2 cut(s) 677, 820
BstKTI GATC 9 cut(s) 223, 601, 650, 751, 793, 1015, 1089, 1112, 1425
BstMAI GTCTC 2 cut(s) 176, 1344
BstMBI GATC 9 cut(s) 220, 598, 647, 748, 790, 1012, 1086, 1109, 1422
BstMWI GCNNNNNNNGC 7 cut(s) 107, 257, 266, 887, 1255, 1310, 1384
BstNI CCWGG 2 cut(s) 237, 1017
BstSCI CCNGG 2 cut(s) 235, 1015
BstUI CGCG 4 cut(s) 101, 675, 806, 1262
BstV1I GCAGC 3 cut(s) 443, 1297, 1300
BstX2I RGATCY 2 cut(s) 647, 748
BstYI RGATCY 2 cut(s) 647, 748
BsuRI GGCC 1 cut(s) 939
BtgI CCRYGG 1 cut(s) 1379
BtgZI GCGATG 1 cut(s) 656
BtsCI GGATG 5 cut(s) 527, 883, 1153, 1259, 1375
BtsIMutI CAGTG 2 cut(s) 1082, 1398
BtuMI TCGCGA 1 cut(s) 101
Cac8I GCNNGC 2 cut(s) 998, 1260
CfoI GCGC 2 cut(s) 677, 820
Cfr13I GGNCC 3 cut(s) 314, 686, 938
CseI GACGC 3 cut(s) 812, 1251, 1361
Csp6I GTAC 3 cut(s) 501, 588, 1405
CspCI CAANNNNNGTGG 4 cut(s) 283, 318, 1398, 1433
CviAII CATG 6 cut(s) 111, 266, 311, 389, 1273, 1380
CviQI GTAC 3 cut(s) 501, 588, 1405
DdeI CTNAG 7 cut(s) 159, 1031, 1074, 1278, 1305, 1331, 1401
DpnI GATC 9 cut(s) 222, 600, 649, 750, 792, 1014, 1088, 1111, 1424
DpnII GATC 9 cut(s) 220, 598, 647, 748, 790, 1012, 1086, 1109, 1422
DraI TTTAAA 1 cut(s) 765
DraIII CACNNNGTG 1 cut(s) 1214
Eam1104I CTCTTC 2 cut(s) 1077, 1184
EarI CTCTTC 2 cut(s) 1077, 1184
Ecl136II GAGCTC 1 cut(s) 1282
Eco130I CCWWGG 2 cut(s) 1157, 1379
Eco24I GRGCYC 1 cut(s) 1284
Eco47I GGWCC 2 cut(s) 314, 686
Eco53kI GAGCTC 1 cut(s) 1282
Eco57I CTGAAG 1 cut(s) 795
EcoICRI GAGCTC 1 cut(s) 1282
EcoO109I RGGNCCY 1 cut(s) 938
EcoRII CCWGG 2 cut(s) 235, 1015
EcoT14I CCWWGG 2 cut(s) 1157, 1379
EcoT22I ATGCAT 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 1284
ErhI CCWWGG 2 cut(s) 1157, 1379
Esp3I CGTCTC 2 cut(s) 176, 1344
FaeI CATG 6 cut(s) 114, 269, 314, 392, 1276, 1383
FalI AAGNNNNNCTT 8 cut(s) 120, 152, 318, 350, 1141, 1173, 1181, 1213
FaqI GGGAC 2 cut(s) 1041, 1079
FatI CATG 6 cut(s) 110, 265, 310, 388, 1272, 1379
FauI CCCGC 2 cut(s) 778, 799
FauNDI CATATG 1 cut(s) 892
FbaI TGATCA 2 cut(s) 220, 790
Fnu4HI GCNGC 4 cut(s) 386, 432, 1311, 1314
FokI GGATG 5 cut(s) 534, 890, 1140, 1266, 1382
FriOI GRGCYC 1 cut(s) 1284
Fsp4HI GCNGC 4 cut(s) 386, 432, 1311, 1314
FspBI CTAG 2 cut(s) 696, 1184
GlaI GCGC 2 cut(s) 676, 819
GluI GCNGC 4 cut(s) 386, 432, 1311, 1314
GsuI CTGGAG 2 cut(s) 756, 1366
HaeIII GGCC 1 cut(s) 939
HapII CCGG 2 cut(s) 11, 1175
HgaI GACGC 3 cut(s) 812, 1251, 1361
HhaI GCGC 2 cut(s) 677, 820
Hin1II CATG 6 cut(s) 114, 269, 314, 392, 1276, 1383
Hin6I GCGC 2 cut(s) 675, 818
HinP1I GCGC 2 cut(s) 675, 818
HincII GTYRAC 2 cut(s) 1009, 1066
HindII GTYRAC 2 cut(s) 1009, 1066
HinfI GANTC 6 cut(s) 5, 71, 96, 103, 203, 1126
HpaI GTTAAC 1 cut(s) 1066
HpaII CCGG 2 cut(s) 11, 1175
HphI GGTGA 3 cut(s) 506, 1352, 1385
Hpy166II GTNNAC 3 cut(s) 95, 1009, 1066
Hpy188I TCNGA 2 cut(s) 144, 1449
Hpy188III TCNNGA 7 cut(s) 54, 100, 218, 735, 1096, 1184, 1426
Hpy8I GTNNAC 3 cut(s) 95, 1009, 1066
HpyAV CCTTC 3 cut(s) 431, 836, 1423
HpyCH4III ACNGT 3 cut(s) 188, 342, 505
HpyCH4V TGCA 9 cut(s) 29, 452, 462, 488, 830, 890, 978, 996, 1363
HpyF10VI GCNNNNNNNGC 7 cut(s) 107, 257, 266, 887, 1255, 1310, 1384
HpyF3I CTNAG 7 cut(s) 159, 1031, 1074, 1278, 1305, 1331, 1401
Hsp92II CATG 6 cut(s) 114, 269, 314, 392, 1276, 1383
HspAI GCGC 2 cut(s) 675, 818
Ksp22I TGATCA 2 cut(s) 220, 790
KspAI GTTAAC 1 cut(s) 1066
Kzo9I GATC 9 cut(s) 220, 598, 647, 748, 790, 1012, 1086, 1109, 1422
LmnI GCTCC 2 cut(s) 265, 274
Lsp1109I GCAGC 3 cut(s) 443, 1297, 1300
LweI GCATC 5 cut(s) 16, 38, 144, 805, 868
MaeI CTAG 2 cut(s) 696, 1184
MaeIII GTNAC 2 cut(s) 182, 1340
MalI GATC 9 cut(s) 222, 600, 649, 750, 792, 1014, 1088, 1111, 1424
MboI GATC 9 cut(s) 220, 598, 647, 748, 790, 1012, 1086, 1109, 1422
MboII GAAGA 8 cut(s) 158, 224, 724, 788, 899, 1094, 1121, 1201
MfeI CAATTG 1 cut(s) 1113
MflI RGATCY 2 cut(s) 647, 748
MhlI GDGCHC 2 cut(s) 1284, 1400
MluCI AATT 7 cut(s) 41, 295, 558, 810, 856, 1047, 1113
MluI ACGCGT 1 cut(s) 1260
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 550
Mph1103I ATGCAT 1 cut(s) 31
MseI TTAA 9 cut(s) 288, 324, 537, 657, 764, 863, 1022, 1065, 1245
MslI CAYNNNNRTG 4 cut(s) 377, 872, 1137, 1215
MspA1I CMGCKG 3 cut(s) 434, 708, 773
MspCI CTTAAG 1 cut(s) 862
MspI CCGG 2 cut(s) 11, 1175
MspR9I CCNGG 2 cut(s) 237, 1017
MunI CAATTG 1 cut(s) 1113
Mva1269I GAATGC 2 cut(s) 464, 1355
MvaI CCWGG 2 cut(s) 237, 1017
MvnI CGCG 4 cut(s) 101, 675, 806, 1262
MwoI GCNNNNNNNGC 7 cut(s) 107, 257, 266, 887, 1255, 1310, 1384
NcoI CCATGG 1 cut(s) 1379
NdeI CATATG 1 cut(s) 892
NdeII GATC 9 cut(s) 220, 598, 647, 748, 790, 1012, 1086, 1109, 1422
NlaIII CATG 6 cut(s) 114, 269, 314, 392, 1276, 1383
NlaIV GGNNCC 3 cut(s) 261, 687, 1434
NmeAIII GCCGAG 1 cut(s) 188
NmuCI GTSAC 2 cut(s) 182, 1340
NruI TCGCGA 1 cut(s) 101
NsiI ATGCAT 1 cut(s) 31
OliI CACNNNNGTG 1 cut(s) 377
PctI GAATGC 2 cut(s) 464, 1355
PfeI GAWTC 4 cut(s) 71, 103, 203, 1126
PkrI GCNGC 4 cut(s) 387, 433, 1312, 1315
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 1284
Psp6I CCWGG 2 cut(s) 235, 1015
PspGI CCWGG 2 cut(s) 235, 1015
PspN4I GGNNCC 3 cut(s) 261, 687, 1434
PspPI GGNCC 3 cut(s) 314, 686, 938
PsuI RGATCY 2 cut(s) 647, 748
PvuII CAGCTG 1 cut(s) 708
RruI TCGCGA 1 cut(s) 101
RsaI GTAC 3 cut(s) 502, 589, 1406
RsaNI GTAC 3 cut(s) 501, 588, 1405
RseI CAYNNNNRTG 4 cut(s) 377, 872, 1137, 1215
SacI GAGCTC 1 cut(s) 1284
SaqAI TTAA 9 cut(s) 288, 324, 537, 657, 764, 863, 1022, 1065, 1245
SatI GCNGC 4 cut(s) 386, 432, 1311, 1314
Sau3AI GATC 9 cut(s) 220, 598, 647, 748, 790, 1012, 1086, 1109, 1422
Sau96I GGNCC 3 cut(s) 314, 686, 938
ScaI AGTACT 1 cut(s) 1406
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 2 cut(s) 237, 1017
SduI GDGCHC 2 cut(s) 1284, 1400
SfaNI GCATC 5 cut(s) 16, 38, 144, 805, 868
SinI GGWCC 2 cut(s) 314, 686
SmiMI CAYNNNNRTG 4 cut(s) 377, 872, 1137, 1215
SmlI CTYRAG 3 cut(s) 862, 882, 933
SmoI CTYRAG 3 cut(s) 862, 882, 933
Sse9I AATT 7 cut(s) 41, 295, 558, 810, 856, 1047, 1113
SsiI CCGC 5 cut(s) 386, 434, 771, 806, 1316
SspMI CTAG 2 cut(s) 696, 1184
SstI GAGCTC 1 cut(s) 1284
StyD4I CCNGG 2 cut(s) 235, 1015
StyI CCWWGG 2 cut(s) 1157, 1379
TaaI ACNGT 3 cut(s) 188, 342, 505
TaqI TCGA 2 cut(s) 32, 400
TasI AATT 7 cut(s) 41, 295, 558, 810, 856, 1047, 1113
TatI WGTACW 1 cut(s) 1404
TauI GCSGC 1 cut(s) 388
TfiI GAWTC 4 cut(s) 71, 103, 203, 1126
Tru1I TTAA 9 cut(s) 288, 324, 537, 657, 764, 863, 1022, 1065, 1245
Tru9I TTAA 9 cut(s) 288, 324, 537, 657, 764, 863, 1022, 1065, 1245
TscAI CASTG 2 cut(s) 1082, 1405
TseFI GTSAC 2 cut(s) 182, 1340
TseI GCWGC 3 cut(s) 431, 1310, 1313
Tsp45I GTSAC 2 cut(s) 182, 1340
TspDTI ATGAA 4 cut(s) 274, 459, 855, 1058
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 2 cut(s) 1082, 1405
Vha464I CTTAAG 1 cut(s) 862
VpaK11BI GGWCC 2 cut(s) 314, 686
XapI RAATTY 3 cut(s) 41, 558, 1047
XbaI TCTAGA 1 cut(s) 1183
XspI CTAG 2 cut(s) 696, 1184
ZrmI AGTACT 1 cut(s) 1406
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.