MD13G1262800.v1.1

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011984
Physical Location & Seq
Forward (+)
33398697 .. 33407332
8636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1262800.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGGATTCTCCGTCGTCGTCGACGACGCGGATCACCGCGCGATCGTCGATGATCGACTCTTTCAGGGGCTGTACCCTATCGGGTATGACGATTGACAAGGAGGAGCTCAAGAAGAAGCTCTTGATGCCGCAGTACCTCCGCTTCGCTATGCGCGATTCTATACGGTTAAAGGACCCAAGAGCCGGCGAGAGCCGCCTTCCGGGAAGTCGAGACCTCGTCGCCACCGCCGCCGTCGAAAATGTGGAGGAAACGCCGCCGGAGTCGCCCATGGTTGTGTTTATAAATCCGCGAAGCGGTGGAAGATACGGGCCTATGCTCAAGGAGAGGCTCCAACTGCTGATGGGTGAAGAACAGGTTTTTGACCTCTCAGATGTGAAGCCTCATGAATTTGTTCAGTATGGGTTGGGTTGTCTAGAGTTATTGGCTGATCTTGGTGACCTTTGTGCCAAAGAGTGTCGTCAAAAGATCAGGGTTATGGTTGCAGGGGGTGATGGTACAGTGGGTTGGGTACTTGGTTGCCTATATGAACTCAACAGACAGGGTTTGGAACCGGTTCCTCCAGTAGGAGTTATTCCACTTGGCACGGGAAATGATCTTTCCAGGAGTTTTGGTTGGGGTGGTTCATTCCCTTTTGCATGGAAATCAGCCATTAAAAAAACTCTATTCAGGGCTACTGCAGGTCCAATATGCCGTTTGGATAGTTGGCATATTGTGCTGTCAATGCCAGCCGGTACAGAAATGGACCCACCCCATTCTTTGAAGCTTACTGAAGAATGCGCTCTTGATGAGACTGGTTTGGAAAAAGGAGATTTGCCTGAGAAATCTACTTGCCACGAAGGAGTGTTTTATAATTACTTCAGCATAGGAATGGATGCCCAAGTTGCGTATGGATTCCACCATTTACGAAATGAAAAACCGTATCTTGCACAAGGTCCAATTTCAAATAAGTTGGTTTACTCAGGTTATAGTTGCACTCAAGGTTGGTTCTTCACACCTTGCACAAGTGATCCTGGTTTAAGGGGACTAAAGAACATCTTAAGGATGCATGTCAAAAAGGTAAATTGCTCAGAGTGGGAGCAGATTCCAGTGCCTTCAAGCGTACGGGCAATTGTTGCTCTAAATCTTCATAACTATGGGAGTGGAAGAAATCCGTGGGGTAATCTGAAGCCAGAGTACTTGGAAAAGAGAGGCTTCGTGGAGGCCCATGCTGATGATGGCCTTCTTGAAGTTTTTGGCCTGAAACAAGGATGGCATGCATCTTTTGTTATGGTGGAACTCATCTCTGCAAAACACATTGCTCAGGCGGCTGCAATTCGGTTGGAAGTTAGAGGTGGGGAATGGAGAAATGCATATATGCAAATGGATGGGGAACCATGGAAGCAGCCAATGCACGAGGAGTATTCAACGCTGGTGGAAATTACGAGGGTTCCGTTTCAATCACTGATGATTCATGGAGACGATCACTGA

Protein Analysis

489

Amino Acids

54.38

Weight (kDa)

6.19

Isoelectric Point (pI)

48.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 90 - 221 3.5e-22 Diacylglycerol kinase catalytic domain
DAGK_acc PF00609 281 - 458 3.5e-42 Diacylglycerol kinase accessory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18730 AT4G30340 AT4G30340 AT5G57690
fragaria_vesca FvH4_4g08400 FvH4_4g08400
malus_domestica MD13G1262800.v1.1 MD16G1264500.v1.1
prunus_persica Prupe.1G094900_v2.0.a1
pyrus_communis pycom13g24340 pycom16g23310
rosa_chinensis RchiOBHm_Chr4g0404101
rosa_laevigata RLG00000008925
rosa_multiflora Rmu_sc0006579.1_g000001
rosa_roxburghii Rroxscaffold_5G00348640
rosa_rugosa Rorug04G0046900 Rorug04G0047000
rosa_samantha Rh4DG115900
rosa_wichuraiana Rw4G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 281, 849
Acc36I ACCTGC 1 cut(s) 668
AccI GTMKAC 1 cut(s) 20
AccII CGCG 5 cut(s) 28, 38, 40, 153, 289
AclWI GGATC 2 cut(s) 38, 1001
AcsI RAATTY 1 cut(s) 386
AcuI CTGAAG 3 cut(s) 789, 841, 1184
AfaI GTAC 7 cut(s) 73, 134, 496, 510, 733, 1101, 1175
AfiI CCNNNNNNNGG 4 cut(s) 182, 199, 293, 563
AflII CTTAAG 1 cut(s) 1036
AgeI ACCGGT 1 cut(s) 550
AgsI TTSAA 6 cut(s) 760, 942, 1095, 1226, 1404, 1436
AjnI CCWGG 2 cut(s) 599, 1009
AluBI AGCT 3 cut(s) 106, 118, 763
AluI AGCT 3 cut(s) 106, 118, 763
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 3 cut(s) 204, 782, 1449
AlwI GGATC 2 cut(s) 38, 1001
AlwNI CAGNNNCTG 1 cut(s) 69
AoxI GGCC 4 cut(s) 308, 1200, 1216, 1234
ApeKI GCWGC 2 cut(s) 1307, 1381
ApoI RAATTY 1 cut(s) 386
AsiGI ACCGGT 1 cut(s) 550
Asp700I GAANNNNTTC 2 cut(s) 390, 552
AspLEI GCGC 3 cut(s) 40, 153, 779
AspS9I GGNCC 6 cut(s) 172, 308, 680, 742, 932, 1201
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 4 cut(s) 25, 356, 446, 500
AvaII GGWCC 4 cut(s) 172, 680, 742, 932
BanII GRGCYC 1 cut(s) 108
BarI GAAGNNNNNNTAC 4 cut(s) 125, 157, 1157, 1189
BauI CACGAG 1 cut(s) 1391
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 1294, 1393
BccI CCATC 5 cut(s) 334, 485, 1208, 1242, 1358
BceAI ACGGC 2 cut(s) 215, 675
BciT130I CCWGG 2 cut(s) 601, 1011
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 3 cut(s) 204, 782, 1449
BfaI CTAG 1 cut(s) 413
BfmI CTRYAG 1 cut(s) 675
BfrI CTTAAG 1 cut(s) 1036
BfuAI ACCTGC 1 cut(s) 668
BisI GCNGC 7 cut(s) 128, 193, 228, 254, 1305, 1308, 1382
BlsI GCNGC 7 cut(s) 129, 194, 229, 255, 1306, 1309, 1383
BmcAI AGTACT 1 cut(s) 1175
Bme1390I CCNGG 3 cut(s) 201, 601, 1011
Bme18I GGWCC 4 cut(s) 172, 680, 742, 932
BmgT120I GGNCC 6 cut(s) 172, 308, 680, 742, 932, 1201
BmiI GGNNCC 7 cut(s) 174, 329, 549, 555, 744, 1371, 1428
BmrFI CCNGG 3 cut(s) 201, 601, 1011
BmsI GCATC 4 cut(s) 114, 862, 1032, 1265
BpmI CTGGAG 1 cut(s) 543
Bpu10I CCTNAGC 1 cut(s) 1299
BpuEI CTTGAG 3 cut(s) 92, 302, 960
BpuMI CCSGG 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 3 cut(s) 267, 1151, 1373
BsaWI WCCGGW 1 cut(s) 550
BsaXI ACNNNNNCTCC 2 cut(s) 595, 625
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 199, 293, 563
Bse118I RCCGGY 3 cut(s) 182, 550, 728
Bse1I ACTGG 3 cut(s) 560, 796, 1085
Bse3DI GCAATG 1 cut(s) 1293
BseBI CCWGG 2 cut(s) 601, 1011
BseDI CCNNGG 3 cut(s) 267, 1151, 1373
BseGI GGATG 4 cut(s) 877, 1047, 1253, 1369
BseLI CCNNNNNNNGG 4 cut(s) 182, 199, 293, 563
BseMI GCAATG 1 cut(s) 1293
BseMII CTCAG 5 cut(s) 381, 807, 972, 1080, 1313
BseNI ACTGG 3 cut(s) 560, 796, 1085
BseRI GAGGAG 2 cut(s) 116, 1409
BseXI GCAGC 2 cut(s) 1294, 1393
Bsh1236I CGCG 5 cut(s) 28, 38, 40, 153, 289
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 4 cut(s) 310, 1202, 1218, 1236
BshTI ACCGGT 1 cut(s) 550
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 108
BsiSI CCGG 5 cut(s) 183, 200, 257, 551, 729
BsiWI CGTACG 1 cut(s) 1099
BslFI GGGAC 1 cut(s) 1035
BslI CCNNNNNNNGG 4 cut(s) 182, 199, 293, 563
BsmAI GTCTC 3 cut(s) 204, 782, 1449
BsmBI CGTCTC 1 cut(s) 1449
BsmFI GGGAC 1 cut(s) 1035
BsmI GAATGC 1 cut(s) 779
BsnI GGCC 4 cut(s) 310, 1202, 1218, 1236
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 8 cut(s) 30, 41, 51, 427, 465, 592, 1006, 1459
Bsp19I CCATGG 2 cut(s) 267, 1373
BspANI GGCC 4 cut(s) 310, 1202, 1218, 1236
BspCNI CTCAG 5 cut(s) 380, 808, 971, 1079, 1312
BspFNI CGCG 5 cut(s) 28, 38, 40, 153, 289
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 7 cut(s) 174, 329, 549, 555, 744, 1371, 1428
BspMAI CTGCAG 1 cut(s) 679
BspMI ACCTGC 1 cut(s) 668
BspPI GGATC 2 cut(s) 38, 1001
BspTI CTTAAG 1 cut(s) 1036
BspTNI GGTCTC 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 1293
BsrFI RCCGGY 3 cut(s) 182, 550, 728
BsrI ACTGG 3 cut(s) 560, 796, 1085
BssAI RCCGGY 3 cut(s) 182, 550, 728
BssECI CCNNGG 3 cut(s) 267, 1151, 1373
BssMI GATC 8 cut(s) 30, 41, 51, 427, 465, 592, 1006, 1459
BssSI CACGAG 1 cut(s) 1391
BssT1I CCWWGG 2 cut(s) 267, 1373
Bst2BI CACGAG 1 cut(s) 1391
Bst2UI CCWGG 2 cut(s) 601, 1011
Bst4CI ACNGT 3 cut(s) 165, 499, 918
BstAFI CTTAAG 1 cut(s) 1036
BstAPI GCANNNNNTGC 3 cut(s) 712, 1112, 1387
BstC8I GCNNGC 3 cut(s) 184, 726, 1254
BstDEI CTNAG 5 cut(s) 367, 816, 958, 1066, 1299
BstDSI CCRYGG 3 cut(s) 267, 1151, 1373
BstEII GGTNACC 1 cut(s) 434
BstENI CCTNNNNNAGG 1 cut(s) 561
BstF5I GGATG 4 cut(s) 877, 1047, 1253, 1369
BstFNI CGCG 5 cut(s) 28, 38, 40, 153, 289
BstHHI GCGC 3 cut(s) 40, 153, 779
BstKTI GATC 8 cut(s) 33, 44, 54, 430, 468, 595, 1009, 1462
BstMAI GTCTC 3 cut(s) 204, 782, 1449
BstMBI GATC 8 cut(s) 30, 41, 51, 427, 465, 592, 1006, 1459
BstMCI CGRYCG 1 cut(s) 44
BstNI CCWGG 2 cut(s) 601, 1011
BstNSI RCATGY 2 cut(s) 1049, 1256
BstPI GGTNACC 1 cut(s) 434
BstSCI CCNGG 3 cut(s) 199, 599, 1009
BstSFI CTRYAG 1 cut(s) 675
BstUI CGCG 5 cut(s) 28, 38, 40, 153, 289
BstV1I GCAGC 2 cut(s) 1294, 1393
BsuRI GGCC 4 cut(s) 310, 1202, 1218, 1236
BtgI CCRYGG 3 cut(s) 267, 1151, 1373
BtsCI GGATG 4 cut(s) 877, 1047, 1253, 1369
BtsIMutI CAGTG 4 cut(s) 504, 1092, 1439, 1462
BveI ACCTGC 1 cut(s) 668
Cac8I GCNNGC 3 cut(s) 184, 726, 1254
CaiI CAGNNNCTG 1 cut(s) 69
CciI TCATGA 1 cut(s) 382
CfoI GCGC 3 cut(s) 40, 153, 779
Cfr10I RCCGGY 3 cut(s) 182, 550, 728
Cfr13I GGNCC 6 cut(s) 172, 308, 680, 742, 932, 1201
CseI GACGC 1 cut(s) 34
Csp6I GTAC 7 cut(s) 72, 133, 495, 509, 732, 1100, 1174
CspAI ACCGGT 1 cut(s) 550
CspCI CAANNNNNGTGG 2 cut(s) 1392, 1427
CviAII CATG 8 cut(s) 268, 383, 636, 1046, 1205, 1253, 1374, 1451
CviQI GTAC 7 cut(s) 72, 133, 495, 509, 732, 1100, 1174
DdeI CTNAG 5 cut(s) 367, 816, 958, 1066, 1299
DpnI GATC 8 cut(s) 32, 43, 53, 429, 467, 594, 1008, 1461
DpnII GATC 8 cut(s) 30, 41, 51, 427, 465, 592, 1006, 1459
Ecl136II GAGCTC 1 cut(s) 106
Eco130I CCWWGG 2 cut(s) 267, 1373
Eco24I GRGCYC 1 cut(s) 108
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 4 cut(s) 172, 680, 742, 932
Eco53kI GAGCTC 1 cut(s) 106
Eco57I CTGAAG 3 cut(s) 789, 841, 1184
Eco91I GGTNACC 1 cut(s) 434
EcoICRI GAGCTC 1 cut(s) 106
EcoNI CCTNNNNNAGG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 172
EcoO65I GGTNACC 1 cut(s) 434
EcoRII CCWGG 2 cut(s) 599, 1009
EcoT14I CCWWGG 2 cut(s) 267, 1373
EcoT22I ATGCAT 3 cut(s) 1047, 1258, 1351
EcoT38I GRGCYC 1 cut(s) 108
ErhI CCWWGG 2 cut(s) 267, 1373
Esp3I CGTCTC 1 cut(s) 1449
FaeI CATG 8 cut(s) 271, 386, 639, 1049, 1208, 1256, 1377, 1454
FalI AAGNNNNNCTT 6 cut(s) 104, 136, 1019, 1051, 1175, 1207
FaqI GGGAC 1 cut(s) 1035
FatI CATG 8 cut(s) 267, 382, 635, 1045, 1204, 1252, 1373, 1450
FblI GTMKAC 1 cut(s) 20
Fnu4HI GCNGC 7 cut(s) 128, 193, 228, 254, 1305, 1308, 1382
FokI GGATG 4 cut(s) 884, 1054, 1260, 1376
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 7 cut(s) 128, 193, 228, 254, 1305, 1308, 1382
FspBI CTAG 1 cut(s) 413
GlaI GCGC 3 cut(s) 39, 152, 778
GluI GCNGC 7 cut(s) 128, 193, 228, 254, 1305, 1308, 1382
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 4 cut(s) 310, 1202, 1218, 1236
HapII CCGG 5 cut(s) 183, 200, 257, 551, 729
HgaI GACGC 1 cut(s) 34
HhaI GCGC 3 cut(s) 40, 153, 779
Hin1II CATG 8 cut(s) 271, 386, 639, 1049, 1208, 1256, 1377, 1454
Hin6I GCGC 3 cut(s) 38, 151, 777
HinP1I GCGC 3 cut(s) 38, 151, 777
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 7 cut(s) 5, 56, 155, 260, 891, 1081, 1447
HpaII CCGG 5 cut(s) 183, 200, 257, 551, 729
HphI GGTGA 4 cut(s) 25, 356, 446, 500
Hpy166II GTNNAC 2 cut(s) 21, 955
Hpy188I TCNGA 3 cut(s) 370, 1069, 1164
Hpy188III TCNNGA 7 cut(s) 109, 121, 209, 383, 413, 782, 1223
Hpy8I GTNNAC 2 cut(s) 21, 955
Hpy99I CGWCG 8 cut(s) 16, 19, 22, 25, 28, 49, 221, 236
HpyAV CCTTC 4 cut(s) 206, 830, 1101, 1229
HpyCH4III ACNGT 3 cut(s) 165, 499, 918
HpyF3I CTNAG 5 cut(s) 367, 816, 958, 1066, 1299
Hsp92II CATG 8 cut(s) 271, 386, 639, 1049, 1208, 1256, 1377, 1454
HspAI GCGC 3 cut(s) 38, 151, 777
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Kzo9I GATC 8 cut(s) 30, 41, 51, 427, 465, 592, 1006, 1459
LmnI GCTCC 3 cut(s) 103, 333, 1075
Lsp1109I GCAGC 2 cut(s) 1294, 1393
LweI GCATC 4 cut(s) 114, 862, 1032, 1265
MaeI CTAG 1 cut(s) 413
MaeIII GTNAC 1 cut(s) 434
MalI GATC 8 cut(s) 32, 43, 53, 429, 467, 594, 1008, 1461
MboI GATC 8 cut(s) 30, 41, 51, 427, 465, 592, 1006, 1459
MboII GAAGA 7 cut(s) 124, 312, 359, 782, 979, 1115, 1155
MfeI CAATTG 1 cut(s) 1107
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 7 cut(s) 386, 850, 936, 1060, 1107, 1311, 1416
MlyI GAGTC 2 cut(s) 50, 269
MmeI TCCRAC 2 cut(s) 355, 1299
Mph1103I ATGCAT 3 cut(s) 1047, 1258, 1351
MroNI GCCGGC 1 cut(s) 182
MroXI GAANNNNTTC 2 cut(s) 390, 552
MseI TTAA 4 cut(s) 167, 651, 1016, 1037
MslI CAYNNNNRTG 4 cut(s) 272, 866, 1131, 1209
MspCI CTTAAG 1 cut(s) 1036
MspI CCGG 5 cut(s) 183, 200, 257, 551, 729
MspR9I CCNGG 3 cut(s) 201, 601, 1011
MunI CAATTG 1 cut(s) 1107
Mva1269I GAATGC 1 cut(s) 779
MvaI CCWGG 2 cut(s) 601, 1011
MvnI CGCG 5 cut(s) 28, 38, 40, 153, 289
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 2 cut(s) 267, 1373
NdeII GATC 8 cut(s) 30, 41, 51, 427, 465, 592, 1006, 1459
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 8 cut(s) 271, 386, 639, 1049, 1208, 1256, 1377, 1454
NlaIV GGNNCC 7 cut(s) 174, 329, 549, 555, 744, 1371, 1428
NmuCI GTSAC 1 cut(s) 434
NsiI ATGCAT 3 cut(s) 1047, 1258, 1351
NspI RCATGY 2 cut(s) 1049, 1256
PaeI GCATGC 1 cut(s) 1256
PagI TCATGA 1 cut(s) 382
PcsI WCGNNNNNNNCGW 4 cut(s) 20, 86, 150, 1427
PctI GAATGC 1 cut(s) 779
PdiI GCCGGC 1 cut(s) 184
PdmI GAANNNNTTC 2 cut(s) 390, 552
PfeI GAWTC 5 cut(s) 5, 155, 891, 1081, 1447
Pfl23II CGTACG 1 cut(s) 1099
PflFI GACNNNGTC 1 cut(s) 215
PfoI TCCNGGA 2 cut(s) 199, 599
PinAI ACCGGT 1 cut(s) 550
PkrI GCNGC 7 cut(s) 129, 194, 229, 255, 1306, 1309, 1383
Ple19I CGATCG 1 cut(s) 44
PleI GAGTC 2 cut(s) 50, 268
PpsI GAGTC 2 cut(s) 50, 268
PpuMI RGGWCCY 1 cut(s) 172
PsiI TTATAA 2 cut(s) 281, 849
Psp124BI GAGCTC 1 cut(s) 108
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 2 cut(s) 599, 1009
PspEI GGTNACC 1 cut(s) 434
PspGI CCWGG 2 cut(s) 599, 1009
PspLI CGTACG 1 cut(s) 1099
PspN4I GGNNCC 7 cut(s) 174, 329, 549, 555, 744, 1371, 1428
PspPI GGNCC 6 cut(s) 172, 308, 680, 742, 932, 1201
PspPPI RGGWCCY 1 cut(s) 172
PstI CTGCAG 1 cut(s) 679
PstNI CAGNNNCTG 1 cut(s) 69
PsyI GACNNNGTC 1 cut(s) 215
PvuI CGATCG 1 cut(s) 44
RsaI GTAC 7 cut(s) 73, 134, 496, 510, 733, 1101, 1175
RsaNI GTAC 7 cut(s) 72, 133, 495, 509, 732, 1100, 1174
RseI CAYNNNNRTG 4 cut(s) 272, 866, 1131, 1209
SacI GAGCTC 1 cut(s) 108
SalI GTCGAC 1 cut(s) 19
SaqAI TTAA 4 cut(s) 167, 651, 1016, 1037
SatI GCNGC 7 cut(s) 128, 193, 228, 254, 1305, 1308, 1382
Sau3AI GATC 8 cut(s) 30, 41, 51, 427, 465, 592, 1006, 1459
Sau96I GGNCC 6 cut(s) 172, 308, 680, 742, 932, 1201
ScaI AGTACT 1 cut(s) 1175
SchI GAGTC 2 cut(s) 50, 269
ScrFI CCNGG 3 cut(s) 201, 601, 1011
SduI GDGCHC 1 cut(s) 108
SfaNI GCATC 4 cut(s) 114, 862, 1032, 1265
SfcI CTRYAG 1 cut(s) 675
SgrDI CGTCGACG 1 cut(s) 19
SinI GGWCC 4 cut(s) 172, 680, 742, 932
SmiMI CAYNNNNRTG 4 cut(s) 272, 866, 1131, 1209
SmlI CTYRAG 4 cut(s) 107, 317, 975, 1036
SmoI CTYRAG 4 cut(s) 107, 317, 975, 1036
SphI GCATGC 1 cut(s) 1256
Sse9I AATT 7 cut(s) 386, 850, 936, 1060, 1107, 1311, 1416
SspMI CTAG 1 cut(s) 413
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 3 cut(s) 199, 599, 1009
StyI CCWWGG 2 cut(s) 267, 1373
TaaI ACNGT 3 cut(s) 165, 499, 918
TaqI TCGA 5 cut(s) 20, 47, 54, 208, 234
TasI AATT 7 cut(s) 386, 850, 936, 1060, 1107, 1311, 1416
TatI WGTACW 1 cut(s) 1173
TauI GCSGC 5 cut(s) 130, 195, 230, 256, 1307
TfiI GAWTC 5 cut(s) 5, 155, 891, 1081, 1447
Tru1I TTAA 4 cut(s) 167, 651, 1016, 1037
Tru9I TTAA 4 cut(s) 167, 651, 1016, 1037
TscAI CASTG 3 cut(s) 504, 1092, 1446
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 2 cut(s) 1307, 1381
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 6 cut(s) 399, 540, 612, 924, 1115, 1439
TspGWI ACGGA 2 cut(s) 1140, 1419
TspRI CASTG 3 cut(s) 504, 1092, 1446
Tth111I GACNNNGTC 1 cut(s) 215
Vha464I CTTAAG 1 cut(s) 1036
VpaK11BI GGWCC 4 cut(s) 172, 680, 742, 932
XagI CCTNNNNNAGG 1 cut(s) 561
XapI RAATTY 1 cut(s) 386
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 2 cut(s) 1049, 1256
XcmI CCANNNNNNNNNTGG 2 cut(s) 884, 1211
XmiI GTMKAC 1 cut(s) 20
XmnI GAANNNNTTC 2 cut(s) 390, 552
XspI CTAG 1 cut(s) 413
ZrmI AGTACT 1 cut(s) 1175
Zsp2I ATGCAT 3 cut(s) 1047, 1258, 1351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.