Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011984

Physical Location & Seq

Genomic Coordinates Reverse (-)

14838172 .. 14841147

Gene Structure 2976 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G30340.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGGAGGAGACGCCGAGATCAGTCGGCGAAGCATCGACGACTAATTTCGTGGCGGCGAGACCGTCGGCGAAGACCGATGATGCAGTGACGATGAGAGGTTGTGGTTTTGCTAATCTTGCCTTGGTTGGTATAGACAAAGAAGAGCTTCGAGGGAGACTAGCGATGCCTGAGTATCTTCGTATAGCTATGAGAGATTGTATCAAGAGGAAAGATTCAACAGAGATTTCTGATCATTTGTTACTTCCTGGAGGCGCCGCCGCTGATATGGCTCCTCACGCGCCAATGGTTGTGTTTATTAACCCGAAAAGTGGTGGTCGTCATGGTCCTGTTCTTAAAGAGAGGCTTCAACAATTGATGACTGAAGAACAGGTATTTGATCTCACAGAAGTGAAGCCTCATGAATTTGTACGGTACGGTTTGGGGTGCTTGGACACATTGGCTGCTAAAGGAGATGAATGTGCAAGAGAATGCAGAGAAAAGATACGGATCATGGTAGCAGGCGGTGATGGTACCGTTGGTTGGGTTCTTGGATGTCTCGGTGAGCTTCATAAAGATGGTAAATCACATATTCCTCCTGTTGGAGTTATACCTCTTGGTACAGGAAATGACCTCTCCAGGAGTTTTAGTTGGGGTGGTTCATTCCCTTTTGCTTGGAGATCTGCTATGAAAAGAACATTACATCGAGCAACTTTAGGTTCCATTGCTCGATTAGATAGCTGGAAGATTGTAGTGTCGATGCCATCTGGAGAAGTCGTTGATCCTCCTTATTCTTTGAAGCCTACAATAGAGGAAACTGCACTTGATCAGGCTTTGGATGCTGATGGAGATGTACCTCCTAAAGCAAAGTCTTATGAAGGAGTGTTCTACAACTACTTCAGCATAGGTATGGATGCTCAAGTTGCATATGGGTTCCACCATCTTCGCAATGAGAAACCGTATCTAGCTCAAGGCCCTGTTACAAATAAGATTATCTATTCGAGTTACAGTTGTACTCAAGGTTGGTTTTGCACGCCTTGTGTCAACAATCCAGCTTTAAGGGGACTAAGAAACATTATGAAAATCCATATTAAAAAGGCAAATTGTTCCGAGTGGGAAGAGATCCATGTTCCTAAGAGCGTAAGATCTATTGTGGTGTTGAATCTTTATAACTATGGAAGTGGAAGACATCCATGGGGTAATCTCAGACCAAAATATTTAGAGAAGAGAGGATTTGTGGAGGCGCATTGTGATGACGGACTGATAGAGATCTTCGGTTTAAAGCAAGGTTGGCATGCGTCATTTGTAATGGCTGAAATTATCTCAGCTAAGCACATTGCTCAGGCGGCTGCGATTAGATTTGAACTAAGAGGAGGTGATTGGAAAAATGCATTCTTGCAAATGGATGGTGAACCATGGAAACAACCGATGAAGAGTGATTATTCGACTTTTGTAGAGATAAAAAAGGTTCCTTTTCAATCGTTAATGATCAATGGCGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004143 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0022622 GO:0030054 GO:0032501 GO:0032502 GO:0044237 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055044 GO:0071944 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

492

Amino Acids

54.57

Weight (kDa)

7.94

Isoelectric Point (pI)

45.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	95 - 233	3.6e-26	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	287 - 369	3.5e-17	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011984)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18730 AT4G30340 AT4G30340 AT5G57690
fragaria_vesca	FvH4_4g08400 FvH4_4g08400
malus_domestica	MD13G1262800.v1.1 MD16G1264500.v1.1
prunus_persica	Prupe.1G094900_v2.0.a1
pyrus_communis	pycom13g24340 pycom16g23310
rosa_chinensis	RchiOBHm_Chr4g0404101
rosa_laevigata	RLG00000008925
rosa_multiflora	Rmu_sc0006579.1_g000001
rosa_roxburghii	Rroxscaffold_5G00348640
rosa_rugosa	Rorug04G0046900 Rorug04G0047000
rosa_samantha	Rh4DG115900
rosa_wichuraiana	Rw4G009980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1146
Acc65I	GGTACC	1 cut(s)	509
AccB1I	GGYRCC	2 cut(s)	251, 509
AccII	CGCG	1 cut(s)	278
AciI	CCGC	5 cut(s)	53, 255, 258, 501, 1322
AclWI	GGATC	3 cut(s)	494, 752, 1093
AcsI	RAATTY	1 cut(s)	401
AcuI	CTGAAG	2 cut(s)	381, 859
AcyI	GRCGYC	2 cut(s)	11, 252
AfaI	GTAC	6 cut(s)	408, 413, 511, 598, 831, 991
AfiI	CCNNNNNNNGG	3 cut(s)	308, 519, 578
AgsI	TTSAA	6 cut(s)	216, 347, 775, 1138, 1340, 1454
AjnI	CCWGG	2 cut(s)	244, 614
AleI	CACNNNNGTG	1 cut(s)	386
AluBI	AGCT	7 cut(s)	145, 185, 544, 717, 944, 1031, 1304
AluI	AGCT	7 cut(s)	145, 185, 544, 717, 944, 1031, 1304
Alw26I	GTCTC	4 cut(s)	2, 52, 148, 539
AlwI	GGATC	3 cut(s)	494, 752, 1093
AoxI	GGCC	1 cut(s)	949
ApeKI	GCWGC	2 cut(s)	440, 1325
ApoI	RAATTY	1 cut(s)	401
Asp700I	GAANNNNTTC	1 cut(s)	144
Asp718I	GGTACC	1 cut(s)	509
AspLEI	GCGC	3 cut(s)	254, 280, 1222
AspS9I	GGNCC	2 cut(s)	323, 950
AsuHPI	GGTGA	4 cut(s)	515, 551, 1364, 1397
AvaII	GGWCC	1 cut(s)	323
BanI	GGYRCC	2 cut(s)	251, 509
BbsI	GAAGAC	2 cut(s)	77, 1168
BbvI	GCAGC	2 cut(s)	427, 1312
BccI	CCATC	6 cut(s)	500, 548, 748, 815, 924, 1376
BciT130I	CCWGG	2 cut(s)	246, 616
BclI	TGATCA	3 cut(s)	229, 802, 1464
BcoDI	GTCTC	4 cut(s)	2, 52, 148, 539
BfaI	CTAG	2 cut(s)	158, 941
BfoI	RGCGCY	1 cut(s)	255
BglII	AGATCT	3 cut(s)	656, 1121, 1245
BisI	GCNGC	6 cut(s)	54, 255, 258, 441, 1323, 1326
BlpI	GCTNAGC	1 cut(s)	1305
BlsI	GCNGC	6 cut(s)	55, 256, 259, 442, 1324, 1327
Bme1390I	CCNGG	2 cut(s)	246, 616
Bme18I	GGWCC	1 cut(s)	323
BmgT120I	GGNCC	2 cut(s)	323, 950
BmiI	GGNNCC	6 cut(s)	253, 270, 511, 697, 911, 1446
BmrFI	CCNGG	2 cut(s)	246, 616
BmsI	GCATC	6 cut(s)	41, 70, 153, 726, 805, 880
BpiI	GAAGAC	2 cut(s)	77, 1168
BpmI	CTGGAG	3 cut(s)	267, 598, 765
Bpu10I	CCTNAGC	1 cut(s)	1317
Bpu1102I	GCTNAGC	1 cut(s)	1305
BpuEI	CTTGAG	3 cut(s)	879, 930, 978
BsaBI	GATNNNNATC	2 cut(s)	485, 1244
BsaHI	GRCGYC	2 cut(s)	11, 252
BsaI	GGTCTC	1 cut(s)	52
BsaJI	CCNNGG	3 cut(s)	120, 1169, 1391
BsaXI	ACNNNNNCTCC	4 cut(s)	610, 640, 738, 768
Bsc4I	CCNNNNNNNGG	3 cut(s)	308, 519, 578
Bse3DI	GCAATG	3 cut(s)	699, 931, 1311
Bse8I	GATNNNNATC	2 cut(s)	485, 1244
BseBI	CCWGG	2 cut(s)	246, 616
BseDI	CCNNGG	3 cut(s)	120, 1169, 1391
BseGI	GGATG	5 cut(s)	536, 820, 895, 1165, 1387
BseJI	GATNNNNATC	2 cut(s)	485, 1244
BseLI	CCNNNNNNNGG	3 cut(s)	308, 519, 578
BseMI	GCAATG	3 cut(s)	699, 931, 1311
BseMII	CTCAG	4 cut(s)	159, 1195, 1314, 1331
BseRI	GAGGAG	3 cut(s)	20, 261, 1362
BseXI	GCAGC	2 cut(s)	427, 1312
BsgI	GTGCAG	1 cut(s)	780
Bsh1236I	CGCG	1 cut(s)	278
BshFI	GGCC	1 cut(s)	951
BshNI	GGYRCC	2 cut(s)	251, 509
BslFI	GGGAC	1 cut(s)	1053
BslI	CCNNNNNNNGG	3 cut(s)	308, 519, 578
BsmAI	GTCTC	4 cut(s)	2, 52, 148, 539
BsmBI	CGTCTC	1 cut(s)	2
BsmFI	GGGAC	1 cut(s)	1053
BsmI	GAATGC	2 cut(s)	473, 1367
BsnI	GGCC	1 cut(s)	951
Bso31I	GGTCTC	1 cut(s)	52
Bsp1720I	GCTNAGC	1 cut(s)	1305
Bsp19I	CCATGG	2 cut(s)	1169, 1391
BspACI	CCGC	5 cut(s)	53, 255, 258, 501, 1322
BspANI	GGCC	1 cut(s)	951
BspCNI	CTCAG	4 cut(s)	160, 1194, 1313, 1330
BspFNI	CGCG	1 cut(s)	278
BspHI	TCATGA	1 cut(s)	397
BspLI	GGNNCC	6 cut(s)	253, 270, 511, 697, 911, 1446
BspPI	GGATC	3 cut(s)	494, 752, 1093
BspQI	GCTCTTC	1 cut(s)	135
BspT107I	GGYRCC	2 cut(s)	251, 509
BspTNI	GGTCTC	1 cut(s)	52
BsrDI	GCAATG	3 cut(s)	699, 931, 1311
BssECI	CCNNGG	3 cut(s)	120, 1169, 1391
BssNI	GRCGYC	2 cut(s)	11, 252
BssT1I	CCWWGG	3 cut(s)	120, 1169, 1391
Bst2UI	CCWGG	2 cut(s)	246, 616
Bst4CI	ACNGT	6 cut(s)	63, 411, 416, 514, 936, 986
Bst6I	CTCTTC	4 cut(s)	135, 1089, 1196, 1403
BstACI	GRCGYC	2 cut(s)	11, 252
BstC8I	GCNNGC	3 cut(s)	499, 1010, 1272
BstDEI	CTNAG	8 cut(s)	168, 1043, 1110, 1181, 1300, 1305, 1317, 1343
BstDSI	CCRYGG	2 cut(s)	1169, 1391
BstF5I	GGATG	5 cut(s)	536, 820, 895, 1165, 1387
BstFNI	CGCG	1 cut(s)	278
BstH2I	RGCGCY	1 cut(s)	255
BstHHI	GCGC	3 cut(s)	254, 280, 1222
BstMAI	GTCTC	4 cut(s)	2, 52, 148, 539
BstMWI	GCNNNNNNNGC	7 cut(s)	116, 266, 275, 815, 899, 1267, 1322
BstNI	CCWGG	2 cut(s)	246, 616
BstNSI	RCATGY	1 cut(s)	1274
BstSCI	CCNGG	2 cut(s)	244, 614
BstUI	CGCG	1 cut(s)	278
BstV1I	GCAGC	2 cut(s)	427, 1312
BstV2I	GAAGAC	2 cut(s)	77, 1168
BstX2I	RGATCY	4 cut(s)	656, 1098, 1121, 1245
BstYI	RGATCY	4 cut(s)	656, 1098, 1121, 1245
BsuRI	GGCC	1 cut(s)	951
BtgI	CCRYGG	2 cut(s)	1169, 1391
BtgZI	GCGATG	1 cut(s)	176
BtsCI	GGATG	5 cut(s)	536, 820, 895, 1165, 1387
BtsI	GCAGTG	1 cut(s)	90
BtsIMutI	CAGTG	1 cut(s)	90
Cac8I	GCNNGC	3 cut(s)	499, 1010, 1272
CciI	TCATGA	1 cut(s)	397
CfoI	GCGC	3 cut(s)	254, 280, 1222
Cfr13I	GGNCC	2 cut(s)	323, 950
CseI	GACGC	2 cut(s)	19, 1263
Csp6I	GTAC	6 cut(s)	407, 412, 510, 597, 830, 990
CviAII	CATG	7 cut(s)	320, 398, 490, 1103, 1170, 1271, 1392
CviQI	GTAC	6 cut(s)	407, 412, 510, 597, 830, 990
DdeI	CTNAG	8 cut(s)	168, 1043, 1110, 1181, 1300, 1305, 1317, 1343
DinI	GGCGCC	1 cut(s)	253
DraI	TTTAAA	1 cut(s)	1257
Eam1104I	CTCTTC	4 cut(s)	135, 1089, 1196, 1403
EarI	CTCTTC	4 cut(s)	135, 1089, 1196, 1403
Eco130I	CCWWGG	3 cut(s)	120, 1169, 1391
Eco31I	GGTCTC	1 cut(s)	52
Eco47I	GGWCC	1 cut(s)	323
Eco57I	CTGAAG	2 cut(s)	381, 859
EcoO109I	RGGNCCY	1 cut(s)	950
EcoRII	CCWGG	2 cut(s)	244, 614
EcoT14I	CCWWGG	3 cut(s)	120, 1169, 1391
EcoT22I	ATGCAT	1 cut(s)	1369
EgeI	GGCGCC	1 cut(s)	253
EheI	GGCGCC	1 cut(s)	253
ErhI	CCWWGG	3 cut(s)	120, 1169, 1391
Esp3I	CGTCTC	1 cut(s)	2
FaeI	CATG	7 cut(s)	323, 401, 493, 1106, 1173, 1274, 1395
FalI	AAGNNNNNCTT	4 cut(s)	129, 161, 327, 359
FaqI	GGGAC	1 cut(s)	1053
FatI	CATG	7 cut(s)	319, 397, 489, 1102, 1169, 1270, 1391
FauNDI	CATATG	1 cut(s)	904
FbaI	TGATCA	3 cut(s)	229, 802, 1464
Fnu4HI	GCNGC	6 cut(s)	54, 255, 258, 441, 1323, 1326
FokI	GGATG	5 cut(s)	543, 827, 902, 1152, 1394
Fsp4HI	GCNGC	6 cut(s)	54, 255, 258, 441, 1323, 1326
FspBI	CTAG	2 cut(s)	158, 941
GlaI	GCGC	3 cut(s)	253, 279, 1221
GluI	GCNGC	6 cut(s)	54, 255, 258, 441, 1323, 1326
GsuI	CTGGAG	3 cut(s)	267, 598, 765
HaeII	RGCGCY	1 cut(s)	255
HaeIII	GGCC	1 cut(s)	951
HgaI	GACGC	2 cut(s)	19, 1263
HhaI	GCGC	3 cut(s)	254, 280, 1222
Hin1I	GRCGYC	2 cut(s)	11, 252
Hin1II	CATG	7 cut(s)	323, 401, 493, 1106, 1173, 1274, 1395
Hin6I	GCGC	3 cut(s)	252, 278, 1220
HinP1I	GCGC	3 cut(s)	252, 278, 1220
HincII	GTYRAC	1 cut(s)	1021
HindII	GTYRAC	1 cut(s)	1021
HinfI	GANTC	2 cut(s)	212, 1138
HphI	GGTGA	4 cut(s)	515, 551, 1364, 1397
Hpy166II	GTNNAC	2 cut(s)	1021, 1388
Hpy188I	TCNGA	3 cut(s)	229, 1087, 1184
Hpy188III	TCNNGA	3 cut(s)	202, 398, 744
Hpy8I	GTNNAC	2 cut(s)	1021, 1388
Hpy99I	CGWCG	2 cut(s)	40, 67
HpyAV	CCTTC	1 cut(s)	848
HpyCH4III	ACNGT	6 cut(s)	63, 411, 416, 514, 936, 986
HpyCH4V	TGCA	8 cut(s)	83, 461, 471, 797, 902, 1008, 1367, 1375
HpyF10VI	GCNNNNNNNGC	7 cut(s)	116, 266, 275, 815, 899, 1267, 1322
HpyF3I	CTNAG	8 cut(s)	168, 1043, 1110, 1181, 1300, 1305, 1317, 1343
Hsp92I	GRCGYC	2 cut(s)	11, 252
Hsp92II	CATG	7 cut(s)	323, 401, 493, 1106, 1173, 1274, 1395
HspAI	GCGC	3 cut(s)	252, 278, 1220
KasI	GGCGCC	1 cut(s)	251
KpnI	GGTACC	1 cut(s)	513
Ksp22I	TGATCA	3 cut(s)	229, 802, 1464
LguI	GCTCTTC	1 cut(s)	135
LmnI	GCTCC	1 cut(s)	274
Lsp1109I	GCAGC	2 cut(s)	427, 1312
LweI	GCATC	6 cut(s)	41, 70, 153, 726, 805, 880
MaeI	CTAG	2 cut(s)	158, 941
MaeIII	GTNAC	4 cut(s)	85, 237, 955, 980
MfeI	CAATTG	1 cut(s)	350
MflI	RGATCY	4 cut(s)	656, 1098, 1121, 1245
MluCI	AATT	5 cut(s)	43, 350, 401, 1078, 1293
Mly113I	GGCGCC	1 cut(s)	252
MmeI	TCCRAC	1 cut(s)	559
Mph1103I	ATGCAT	1 cut(s)	1369
MroXI	GAANNNNTTC	1 cut(s)	144
MseI	TTAA	6 cut(s)	297, 333, 1034, 1068, 1256, 1460
MslI	CAYNNNNRTG	4 cut(s)	386, 552, 884, 1227
MspA1I	CMGCKG	1 cut(s)	260
MspR9I	CCNGG	2 cut(s)	246, 616
MunI	CAATTG	1 cut(s)	350
Mva1269I	GAATGC	2 cut(s)	473, 1367
MvaI	CCWGG	2 cut(s)	246, 616
MvnI	CGCG	1 cut(s)	278
MwoI	GCNNNNNNNGC	7 cut(s)	116, 266, 275, 815, 899, 1267, 1322
NarI	GGCGCC	1 cut(s)	252
NcoI	CCATGG	2 cut(s)	1169, 1391
NdeI	CATATG	1 cut(s)	904
NlaIII	CATG	7 cut(s)	323, 401, 493, 1106, 1173, 1274, 1395
NlaIV	GGNNCC	6 cut(s)	253, 270, 511, 697, 911, 1446
NmeAIII	GCCGAG	1 cut(s)	39
NmuCI	GTSAC	1 cut(s)	85
NsiI	ATGCAT	1 cut(s)	1369
NspI	RCATGY	1 cut(s)	1274
OliI	CACNNNNGTG	1 cut(s)	386
PaeI	GCATGC	1 cut(s)	1274
PagI	TCATGA	1 cut(s)	397
PciSI	GCTCTTC	1 cut(s)	135
PctI	GAATGC	2 cut(s)	473, 1367
PdmI	GAANNNNTTC	1 cut(s)	144
PfeI	GAWTC	2 cut(s)	212, 1138
PfoI	TCCNGGA	2 cut(s)	244, 614
PkrI	GCNGC	6 cut(s)	55, 256, 259, 442, 1324, 1327
PluTI	GGCGCC	1 cut(s)	255
PsiI	TTATAA	1 cut(s)	1146
Psp6I	CCWGG	2 cut(s)	244, 614
PspGI	CCWGG	2 cut(s)	244, 614
PspN4I	GGNNCC	6 cut(s)	253, 270, 511, 697, 911, 1446
PspPI	GGNCC	2 cut(s)	323, 950
PsuI	RGATCY	4 cut(s)	656, 1098, 1121, 1245
RsaI	GTAC	6 cut(s)	408, 413, 511, 598, 831, 991
RsaNI	GTAC	6 cut(s)	407, 412, 510, 597, 830, 990
RseI	CAYNNNNRTG	4 cut(s)	386, 552, 884, 1227
SapI	GCTCTTC	1 cut(s)	135
SaqAI	TTAA	6 cut(s)	297, 333, 1034, 1068, 1256, 1460
SatI	GCNGC	6 cut(s)	54, 255, 258, 441, 1323, 1326
Sau96I	GGNCC	2 cut(s)	323, 950
ScrFI	CCNGG	2 cut(s)	246, 616
SfaNI	GCATC	6 cut(s)	41, 70, 153, 726, 805, 880
SfoI	GGCGCC	1 cut(s)	253
SinI	GGWCC	1 cut(s)	323
SmiMI	CAYNNNNRTG	4 cut(s)	386, 552, 884, 1227
SmlI	CTYRAG	3 cut(s)	894, 945, 993
SmoI	CTYRAG	3 cut(s)	894, 945, 993
SphI	GCATGC	1 cut(s)	1274
Sse9I	AATT	5 cut(s)	43, 350, 401, 1078, 1293
SsiI	CCGC	5 cut(s)	53, 255, 258, 501, 1322
SspDI	GGCGCC	1 cut(s)	251
SspI	AATATT	1 cut(s)	1193
SspMI	CTAG	2 cut(s)	158, 941
StyD4I	CCNGG	2 cut(s)	244, 614
StyI	CCWWGG	3 cut(s)	120, 1169, 1391
TaaI	ACNGT	6 cut(s)	63, 411, 416, 514, 936, 986
TaqI	TCGA	7 cut(s)	35, 148, 682, 706, 734, 977, 1421
TaqII	GACCGA	1 cut(s)	89
TasI	AATT	5 cut(s)	43, 350, 401, 1078, 1293
TatI	WGTACW	1 cut(s)	989
TauI	GCSGC	4 cut(s)	56, 257, 260, 1325
TfiI	GAWTC	2 cut(s)	212, 1138
Tru1I	TTAA	6 cut(s)	297, 333, 1034, 1068, 1256, 1460
Tru9I	TTAA	6 cut(s)	297, 333, 1034, 1068, 1256, 1460
TscAI	CASTG	1 cut(s)	90
TseFI	GTSAC	1 cut(s)	85
TseI	GCWGC	2 cut(s)	440, 1325
Tsp45I	GTSAC	1 cut(s)	85
TspDTI	ATGAA	8 cut(s)	414, 468, 536, 627, 680, 867, 1070, 1421
TspGWI	ACGGA	2 cut(s)	499, 1248
TspRI	CASTG	1 cut(s)	90
VpaK11BI	GGWCC	1 cut(s)	323
XapI	RAATTY	1 cut(s)	401
XceI	RCATGY	1 cut(s)	1274
XmnI	GAANNNNTTC	1 cut(s)	144
XspI	CTAG	2 cut(s)	158, 941
Zsp2I	ATGCAT	1 cut(s)	1369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.