Rorug04G0046900

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011984
Physical Location & Seq
Reverse (-)
7277285 .. 7278879
1595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0046900.1

Sequence Viewer

Length: 1266 bp
ATGCCTTTTGTTGAGGAAATTTTTACGAAGGCGGAGGTTGATATTATTGCTGGTATCCCGCTTAGTATTCGTGAACAGAGGGATAGATGGGTGTGGCATTATGATAAGAAGGGGATTTATAGTGTCAGAAGTGGTTACCATACTCTCAGACTCATGGAGGATTTTACTAATCGTGCATCGTCATCTGGAAGCTCCGTGAGGGCGTGTGATCTGCACTGGAAAGGATTATGTAAGGCCCGTGTTCCCCCAAAAGTCCGGGTCTTTATGTGGAGGTTGCACAAGGGTATCTTGCCCACTCGAAAGGCGTTGGCTCGTAGGTGTAATATCACGGATACTAGCTGTCCTTTCTGTGGGTTGCATGATGAAGATGACCTGCATGTGTTGACAGAGTGCAAAAGGGTAGAGGAGTTTTGGTTATGTGGTCCACTCAACCTAAAGGTACGCAACATTCAATGCATGACCATTTCTGCATGGATCTTGCATGCATGTGATTCTCTTCCTAAAGACAAATGGGATTTGTTTTATATGTTGTTATGGGCCCTATGGCATGAACGAAATGGTGTTGTATGGAAGGGCAATTCTTTTTGTCCTCTTAATGCTGCAGGCTGGGCTAGCAAACTTCTTGATGATTATCACAAGGCGCACCCAGTGTCTAGAAAGAATAAGTCCAGGACGCAAGTGAAATGGGAGCTGCCCCCGAGGGGTAGACTTAAACTGAACACGGACGGGGCTTTTCATGGAGCAACTGGTTATGGTGGGATCGGGGCCGTTATTAGAAATGAAGATGGTGAGTGTTTAGCTGCAATTGCACGGCCTTTTTCCCGGGTTAGATCAGCTTTCCAAATGGAGGTGGAGGCTATGAGAGCTGGTCTTCTTTTACTTATTCACCAAGGCATGGATAGTGTTGATATTGAGACCGACTGTGCTGCGGTGATTACGGCTCTTCATAGAAATACGGAAGATCTCTCTGAGGTAGGTTGTATAGTGGAGGATTGTAAGGCCTATTTGAGCTCACTTTCCTCCTTTACTCTACGATCTATTTATCGTGAAGCAAATGGTGTGGCCCATAGATTGGCGCATCTTGCTAGTGTTGAGTGGTTAGATGATTATTGGTTAGAGGAGACTCCTGTGATGATCCGGGATGTACTCTACGAGGATTCATGTATTAGTACTCGAGGTTCAGGTTATATGTCCCCCTCGCAGTATAATTATCTTTTAATTAATAATAATAATAATAACGGGCGTGGGGCTGAGCCTCCCAGTTAG

Protein Analysis

421

Amino Acids

47.85

Weight (kDa)

7.88

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 67 - 138 9.8e-13 zinc-binding in reverse transcriptase
RVT_3 PF13456 240 - 361 5.8e-24 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18730 AT4G30340 AT4G30340 AT5G57690
fragaria_vesca FvH4_4g08400 FvH4_4g08400
malus_domestica MD13G1262800.v1.1 MD16G1264500.v1.1
prunus_persica Prupe.1G094900_v2.0.a1
pyrus_communis pycom13g24340 pycom16g23310
rosa_chinensis RchiOBHm_Chr4g0404101
rosa_laevigata RLG00000008925
rosa_multiflora Rmu_sc0006579.1_g000001
rosa_roxburghii Rroxscaffold_5G00348640
rosa_rugosa Rorug04G0046900 Rorug04G0047000
rosa_samantha Rh4DG115900
rosa_wichuraiana Rw4G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 381
AccB7I CCANNNNNTGG 2 cut(s) 895, 1072
AccI GTMKAC 1 cut(s) 706
AciI CCGC 3 cut(s) 32, 59, 929
AclWI GGATC 3 cut(s) 482, 767, 1129
AcsI RAATTY 1 cut(s) 18
AdeI CACNNNGTG 1 cut(s) 649
AfaI GTAC 3 cut(s) 441, 1146, 1171
AfiI CCNNNNNNNGG 5 cut(s) 350, 701, 847, 895, 1072
AgsI TTSAA 1 cut(s) 452
AjnI CCWGG 1 cut(s) 668
AluBI AGCT 7 cut(s) 192, 339, 691, 800, 836, 866, 1011
AluI AGCT 7 cut(s) 192, 339, 691, 800, 836, 866, 1011
Alw21I GWGCWC 1 cut(s) 1013
Alw26I GTCTC 2 cut(s) 908, 1115
AlwI GGATC 3 cut(s) 482, 767, 1129
Ama87I CYCGRG 3 cut(s) 697, 822, 1173
AoxI GGCC 6 cut(s) 234, 537, 765, 812, 999, 1062
ApaI GGGCCC 1 cut(s) 541
ApeKI GCWGC 4 cut(s) 599, 691, 800, 926
ApoI RAATTY 1 cut(s) 18
AseI ATTAAT 1 cut(s) 1221
AspLEI GCGC 2 cut(s) 643, 1078
AspS9I GGNCC 6 cut(s) 235, 422, 537, 538, 765, 1063
AsuC2I CCSGG 4 cut(s) 257, 823, 824, 1139
AsuHPI GGTGA 3 cut(s) 800, 878, 943
AsuNHI GCTAGC 1 cut(s) 611
AvaI CYCGRG 3 cut(s) 697, 822, 1173
AvaII GGWCC 1 cut(s) 422
BaeGI GKGCMC 1 cut(s) 541
BaeI ACNNNNGTAYC 2 cut(s) 268, 301
BanII GRGCYC 2 cut(s) 541, 1013
BbsI GAAGAC 1 cut(s) 863
Bbv12I GWGCWC 1 cut(s) 1013
BbvI GCAGC 4 cut(s) 586, 678, 787, 913
BccI CCATC 2 cut(s) 81, 779
BceAI ACGGC 3 cut(s) 752, 827, 954
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BciT130I CCWGG 1 cut(s) 670
BciVI GTATCC 2 cut(s) 65, 325
BcnI CCSGG 4 cut(s) 257, 823, 824, 1139
BcoDI GTCTC 2 cut(s) 908, 1115
BfaI CTAG 4 cut(s) 336, 612, 654, 1086
BfmI CTRYAG 1 cut(s) 600
BfuAI ACCTGC 1 cut(s) 381
BfuI GTATCC 2 cut(s) 65, 325
BglII AGATCT 1 cut(s) 961
BisI GCNGC 4 cut(s) 600, 692, 801, 927
BlpI GCTNAGC 1 cut(s) 1251
BlsI GCNGC 4 cut(s) 601, 693, 802, 928
BmcAI AGTACT 1 cut(s) 1171
Bme1390I CCNGG 5 cut(s) 257, 670, 823, 824, 1139
Bme18I GGWCC 1 cut(s) 422
BmeT110I CYCGRG 3 cut(s) 697, 822, 1173
BmgT120I GGNCC 6 cut(s) 235, 422, 537, 538, 765, 1063
BmiI GGNNCC 2 cut(s) 539, 766
BmrFI CCNGG 5 cut(s) 257, 670, 823, 824, 1139
BmrI ACTGGG 2 cut(s) 641, 1254
BmsI GCATC 2 cut(s) 185, 1087
BmtI GCTAGC 1 cut(s) 615
BmuI ACTGGG 2 cut(s) 641, 1254
BpiI GAAGAC 1 cut(s) 863
Bpu1102I GCTNAGC 1 cut(s) 1251
BpuMI CCSGG 4 cut(s) 257, 823, 824, 1139
BsaBI GATNNNNATC 1 cut(s) 630
BsaI GGTCTC 1 cut(s) 908
BsaJI CCNNGG 3 cut(s) 698, 822, 889
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 5 cut(s) 350, 701, 847, 895, 1072
Bse1I ACTGG 4 cut(s) 221, 647, 751, 1260
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 1 cut(s) 670
BseDI CCNNGG 3 cut(s) 698, 822, 889
BseGI GGATG 1 cut(s) 1147
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 5 cut(s) 350, 701, 847, 895, 1072
BseMII CTCAG 3 cut(s) 160, 960, 1242
BseNI ACTGG 4 cut(s) 221, 647, 751, 1260
BseRI GAGGAG 2 cut(s) 419, 1133
BseSI GKGCMC 1 cut(s) 541
BseXI GCAGC 4 cut(s) 586, 678, 787, 913
BseYI CCCAGC 1 cut(s) 606
BsgI GTGCAG 1 cut(s) 197
BshFI GGCC 6 cut(s) 236, 539, 767, 814, 1001, 1064
BsiHKAI GWGCWC 1 cut(s) 1013
BsiHKCI CYCGRG 3 cut(s) 697, 822, 1173
BsiSI CCGG 3 cut(s) 256, 823, 1138
BslFI GGGAC 1 cut(s) 1177
BslI CCNNNNNNNGG 5 cut(s) 350, 701, 847, 895, 1072
BsmAI GTCTC 2 cut(s) 908, 1115
BsmFI GGGAC 1 cut(s) 1177
BsnI GGCC 6 cut(s) 236, 539, 767, 814, 1001, 1064
Bso31I GGTCTC 1 cut(s) 908
BsoBI CYCGRG 3 cut(s) 697, 822, 1173
Bsp120I GGGCCC 1 cut(s) 537
Bsp1286I GDGCHC 2 cut(s) 541, 1013
Bsp143I GATC 7 cut(s) 208, 474, 759, 830, 961, 1034, 1134
Bsp1720I GCTNAGC 1 cut(s) 1251
BspACI CCGC 3 cut(s) 32, 59, 929
BspANI GGCC 6 cut(s) 236, 539, 767, 814, 1001, 1064
BspCNI CTCAG 3 cut(s) 159, 961, 1243
BspLI GGNNCC 2 cut(s) 539, 766
BspMAI CTGCAG 1 cut(s) 604
BspMI ACCTGC 1 cut(s) 381
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 3 cut(s) 482, 767, 1129
BspQI GCTCTTC 1 cut(s) 948
BspTNI GGTCTC 1 cut(s) 908
BsrI ACTGG 4 cut(s) 221, 647, 751, 1260
BssECI CCNNGG 3 cut(s) 698, 822, 889
BssMI GATC 7 cut(s) 208, 474, 759, 830, 961, 1034, 1134
BssT1I CCWWGG 1 cut(s) 889
Bst2UI CCWGG 1 cut(s) 670
Bst4CI ACNGT 1 cut(s) 923
Bst6I CTCTTC 2 cut(s) 501, 948
BstC8I GCNNGC 3 cut(s) 483, 604, 613
BstDEI CTNAG 4 cut(s) 62, 146, 969, 1251
BstEII GGTNACC 1 cut(s) 134
BstF5I GGATG 1 cut(s) 1147
BstHHI GCGC 2 cut(s) 643, 1078
BstKTI GATC 7 cut(s) 211, 477, 762, 833, 964, 1037, 1137
BstMAI GTCTC 2 cut(s) 908, 1115
BstMBI GATC 7 cut(s) 208, 474, 759, 830, 961, 1034, 1134
BstMWI GCNNNNNNNGC 5 cut(s) 608, 612, 806, 863, 1082
BstNI CCWGG 1 cut(s) 670
BstNSI RCATGY 3 cut(s) 380, 485, 489
BstPI GGTNACC 1 cut(s) 134
BstSCI CCNGG 5 cut(s) 255, 668, 821, 822, 1137
BstSFI CTRYAG 1 cut(s) 600
BstSLI GKGCMC 1 cut(s) 541
BstV1I GCAGC 4 cut(s) 586, 678, 787, 913
BstV2I GAAGAC 1 cut(s) 863
BstX2I RGATCY 2 cut(s) 474, 961
BstYI RGATCY 2 cut(s) 474, 961
BsuI GTATCC 2 cut(s) 65, 325
BsuRI GGCC 6 cut(s) 236, 539, 767, 814, 1001, 1064
BtsCI GGATG 1 cut(s) 1147
BtsIMutI CAGTG 2 cut(s) 214, 654
BveI ACCTGC 1 cut(s) 381
Cac8I GCNNGC 3 cut(s) 483, 604, 613
CfoI GCGC 2 cut(s) 643, 1078
Cfr13I GGNCC 6 cut(s) 235, 422, 537, 538, 765, 1063
Cfr9I CCCGGG 1 cut(s) 822
CseI GACGC 1 cut(s) 682
Csp6I GTAC 3 cut(s) 440, 1145, 1170
CspCI CAANNNNNGTGG 2 cut(s) 1041, 1076
CviQI GTAC 3 cut(s) 440, 1145, 1170
DdeI CTNAG 4 cut(s) 62, 146, 969, 1251
DpnI GATC 7 cut(s) 210, 476, 761, 832, 963, 1036, 1136
DpnII GATC 7 cut(s) 208, 474, 759, 830, 961, 1034, 1134
DraIII CACNNNGTG 1 cut(s) 649
Eam1104I CTCTTC 2 cut(s) 501, 948
EarI CTCTTC 2 cut(s) 501, 948
EciI GGCGGA 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 1011
Eco130I CCWWGG 1 cut(s) 889
Eco147I AGGCCT 1 cut(s) 1001
Eco24I GRGCYC 2 cut(s) 541, 1013
Eco31I GGTCTC 1 cut(s) 908
Eco47I GGWCC 1 cut(s) 422
Eco53kI GAGCTC 1 cut(s) 1011
Eco88I CYCGRG 3 cut(s) 697, 822, 1173
Eco91I GGTNACC 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 1011
EcoO109I RGGNCCY 1 cut(s) 538
EcoO65I GGTNACC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 668
EcoT14I CCWWGG 1 cut(s) 889
EcoT22I ATGCAT 2 cut(s) 458, 487
EcoT38I GRGCYC 2 cut(s) 541, 1013
ErhI CCWWGG 1 cut(s) 889
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 1 cut(s) 1177
FauI CCCGC 1 cut(s) 66
FblI GTMKAC 1 cut(s) 706
Fnu4HI GCNGC 4 cut(s) 600, 692, 801, 927
FokI GGATG 1 cut(s) 1154
FriOI GRGCYC 2 cut(s) 541, 1013
Fsp4HI GCNGC 4 cut(s) 600, 692, 801, 927
FspBI CTAG 4 cut(s) 336, 612, 654, 1086
GlaI GCGC 2 cut(s) 642, 1077
GluI GCNGC 4 cut(s) 600, 692, 801, 927
GsaI CCCAGC 1 cut(s) 610
HaeIII GGCC 6 cut(s) 236, 539, 767, 814, 1001, 1064
HapII CCGG 3 cut(s) 256, 823, 1138
HgaI GACGC 1 cut(s) 682
HhaI GCGC 2 cut(s) 643, 1078
Hin6I GCGC 2 cut(s) 641, 1076
HinP1I GCGC 2 cut(s) 641, 1076
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 4 cut(s) 150, 491, 1123, 1157
HpaII CCGG 3 cut(s) 256, 823, 1138
HphI GGTGA 3 cut(s) 800, 878, 943
Hpy166II GTNNAC 4 cut(s) 74, 384, 425, 707
Hpy188I TCNGA 3 cut(s) 128, 149, 970
Hpy188III TCNNGA 5 cut(s) 71, 186, 623, 654, 1046
Hpy8I GTNNAC 4 cut(s) 74, 384, 425, 707
HpyAV CCTTC 3 cut(s) 22, 103, 565
HpyCH4III ACNGT 1 cut(s) 923
HpyF10VI GCNNNNNNNGC 5 cut(s) 608, 612, 806, 863, 1082
HpyF3I CTNAG 4 cut(s) 62, 146, 969, 1251
HspAI GCGC 2 cut(s) 641, 1076
Kzo9I GATC 7 cut(s) 208, 474, 759, 830, 961, 1034, 1134
LguI GCTCTTC 1 cut(s) 948
LmnI GCTCC 3 cut(s) 197, 688, 740
Lsp1109I GCAGC 4 cut(s) 586, 678, 787, 913
LweI GCATC 2 cut(s) 185, 1087
MaeI CTAG 4 cut(s) 336, 612, 654, 1086
MaeIII GTNAC 1 cut(s) 134
MalI GATC 7 cut(s) 210, 476, 761, 832, 963, 1036, 1136
MboI GATC 7 cut(s) 208, 474, 759, 830, 961, 1034, 1134
MboII GAAGA 6 cut(s) 377, 488, 794, 863, 935, 971
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 2 cut(s) 474, 961
MhlI GDGCHC 2 cut(s) 541, 1013
MluCI AATT 5 cut(s) 18, 577, 804, 1207, 1218
MlyI GAGTC 2 cut(s) 144, 1117
Mph1103I ATGCAT 2 cut(s) 458, 487
MseI TTAA 4 cut(s) 594, 711, 1217, 1221
MslI CAYNNNNRTG 1 cut(s) 486
MspI CCGG 3 cut(s) 256, 823, 1138
MspR9I CCNGG 5 cut(s) 257, 670, 823, 824, 1139
MunI CAATTG 1 cut(s) 804
MvaI CCWGG 1 cut(s) 670
MwoI GCNNNNNNNGC 5 cut(s) 608, 612, 806, 863, 1082
NciI CCSGG 4 cut(s) 257, 823, 824, 1139
NdeII GATC 7 cut(s) 208, 474, 759, 830, 961, 1034, 1134
NheI GCTAGC 1 cut(s) 611
NlaIV GGNNCC 2 cut(s) 539, 766
NsiI ATGCAT 2 cut(s) 458, 487
NspI RCATGY 3 cut(s) 380, 485, 489
PacI TTAATTAA 1 cut(s) 1221
PaeI GCATGC 1 cut(s) 485
PaeR7I CTCGAG 1 cut(s) 1173
PceI AGGCCT 1 cut(s) 1001
PciSI GCTCTTC 1 cut(s) 948
PfeI GAWTC 2 cut(s) 491, 1157
PflMI CCANNNNNTGG 2 cut(s) 895, 1072
PfoI TCCNGGA 2 cut(s) 668, 1137
PkrI GCNGC 4 cut(s) 601, 693, 802, 928
PleI GAGTC 2 cut(s) 144, 1117
PpsI GAGTC 2 cut(s) 144, 1117
PshBI ATTAAT 1 cut(s) 1221
Psp124BI GAGCTC 1 cut(s) 1013
Psp6I CCWGG 1 cut(s) 668
PspEI GGTNACC 1 cut(s) 134
PspFI CCCAGC 1 cut(s) 606
PspGI CCWGG 1 cut(s) 668
PspN4I GGNNCC 2 cut(s) 539, 766
PspOMI GGGCCC 1 cut(s) 537
PspPI GGNCC 6 cut(s) 235, 422, 537, 538, 765, 1063
PspXI VCTCGAGB 1 cut(s) 1173
PsrI GAACNNNNNNTAC 2 cut(s) 1162, 1194
PstI CTGCAG 1 cut(s) 604
PsuI RGATCY 2 cut(s) 474, 961
RsaI GTAC 3 cut(s) 441, 1146, 1171
RsaNI GTAC 3 cut(s) 440, 1145, 1170
RseI CAYNNNNRTG 1 cut(s) 486
SacI GAGCTC 1 cut(s) 1013
SapI GCTCTTC 1 cut(s) 948
SaqAI TTAA 4 cut(s) 594, 711, 1217, 1221
SatI GCNGC 4 cut(s) 600, 692, 801, 927
Sau3AI GATC 7 cut(s) 208, 474, 759, 830, 961, 1034, 1134
Sau96I GGNCC 6 cut(s) 235, 422, 537, 538, 765, 1063
ScaI AGTACT 1 cut(s) 1171
SchI GAGTC 2 cut(s) 144, 1117
ScrFI CCNGG 5 cut(s) 257, 670, 823, 824, 1139
SduI GDGCHC 2 cut(s) 541, 1013
SfaNI GCATC 2 cut(s) 185, 1087
SfcI CTRYAG 1 cut(s) 600
Sfr274I CTCGAG 1 cut(s) 1173
SinI GGWCC 1 cut(s) 422
SlaI CTCGAG 1 cut(s) 1173
SmaI CCCGGG 1 cut(s) 824
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 1 cut(s) 1173
SmoI CTYRAG 1 cut(s) 1173
SphI GCATGC 1 cut(s) 485
Sse9I AATT 5 cut(s) 18, 577, 804, 1207, 1218
SseBI AGGCCT 1 cut(s) 1001
SsiI CCGC 3 cut(s) 32, 59, 929
SspMI CTAG 4 cut(s) 336, 612, 654, 1086
SstI GAGCTC 1 cut(s) 1013
StuI AGGCCT 1 cut(s) 1001
StyD4I CCNGG 5 cut(s) 255, 668, 821, 822, 1137
StyI CCWWGG 1 cut(s) 889
TaaI ACNGT 1 cut(s) 923
TaqI TCGA 2 cut(s) 298, 1174
TaqII GACCGA 1 cut(s) 932
TasI AATT 5 cut(s) 18, 577, 804, 1207, 1218
TatI WGTACW 2 cut(s) 1144, 1169
TfiI GAWTC 2 cut(s) 491, 1157
Tru1I TTAA 4 cut(s) 594, 711, 1217, 1221
Tru9I TTAA 4 cut(s) 594, 711, 1217, 1221
TscAI CASTG 2 cut(s) 221, 654
TseI GCWGC 4 cut(s) 599, 691, 800, 926
TspDTI ATGAA 6 cut(s) 378, 564, 725, 795, 935, 1149
TspGWI ACGGA 4 cut(s) 184, 344, 737, 971
TspMI CCCGGG 1 cut(s) 822
TspRI CASTG 2 cut(s) 221, 654
Van91I CCANNNNNTGG 2 cut(s) 895, 1072
VpaK11BI GGWCC 1 cut(s) 422
VspI ATTAAT 1 cut(s) 1221
XapI RAATTY 1 cut(s) 18
XbaI TCTAGA 1 cut(s) 653
XceI RCATGY 3 cut(s) 380, 485, 489
XhoI CTCGAG 1 cut(s) 1173
XmaI CCCGGG 1 cut(s) 822
XmiI GTMKAC 1 cut(s) 706
XspI CTAG 4 cut(s) 336, 612, 654, 1086
ZrmI AGTACT 1 cut(s) 1171
Zsp2I ATGCAT 2 cut(s) 458, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.