pycom13g24340

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011984
Physical Location & Seq
Reverse (-)
21501305 .. 21508976
7672 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g24340.10

Sequence Viewer

Length: 1386 bp
ATGACGATTGACAAGGAGGAGCTCAAGAAGAAGCTCTTGATGCCGCAGTACCTCCGCTTCGCTATGCGCGATTCTATACGGTTAAAGGACCCAAAAGCCGGCGAGAGCCGCCTTCCGGGAAGTCGAGACCTCGTCGCCACCGCCGCCGTCGAAAATGTGGAGGAAACGCCGCCGGAGTCGCCCATGGTTGTGTTTATAAATCCGCGAAGCGGTGGAAGATACGGGCCTATGCTCAAGGAGAGGCTCCAATTGCTGATGGGTGAAGAACAGGTTTTTGACCTCTCAGATGTGAAGCCTCATGAATTTGTTCAGTATGGGTTGGGTTGTCTAGAGTTATTGGCTGATGTTGGTGACGTTTGTGCCAAAGAGTGTCGTCAAAAGATCAGGGTTATGGTTGCAGGGGGTGATGGTACAGTCGGTTGGGTACTTGGTTGCCTATATGAACTCAACAGACAGGGTTTGGAACCAGTTCCTCCAGTAGGAGTTATTCCACTTGGCACAGGAAATGATCTTTCCAGGAGTTTTGGTTGGGGTGGTTCATTCCCTTTTGCATGGAAATCAGCCATTAAAAAAACTCTATTCAGGGCTACTGCAGGTCCAATATGCCGTTTGGATAGTTGGCATATTGTGCTGTCAATGCCAGCGGGTACAGAAATGGAGCCACCCCATTCTTTGAAGCTTACTGAAGAATGCGCTCTTGATGAGACTGGTTTGGAAAAAGGGGATTTGCCTGAGAAATCTACTTGCCACGAAGGAGTGTTTTATAATTACTTCAGCATAGGAATGGATGCCCAAGTTGCTTATGGATTCCACCATTTACGAAACGAAAAACCGTATCTTGCACAAGGTCCAATTTCAAATAAGTTGGTTTACTCAGGTTATAGTTGCACTCAAGGTTGGTTCTTCACACCTTGCATATTGATTGATCCTGGTTTAAGGGGACTAAAGAACATCTTAAGGATGCATGTCAAAAAGGTAAATTGCTCAGAGTGGGAGCAGATTTCAGTGCCTTCAAGCGTACGGGCAATTGTTGCACTAAATCTTCATAACTATGGGAGTGGAAGAAATCCGTGGGGTAATCTGAAGCCAGAGTACTTGGAAAAGAGAGGCTTCGTGGAGGCCCATGCTGATGATGGCCTTCTTGAAATTTTTGGCCTGAAACAAGGATGGCATGCATCTTTTGTTATGGTGGAACTCGTCTCTGCAAAACACATTGCTCAGGCGGCTGCAATTCGGTTGGAAGTTAGAGGTGGGGAATGGAGAAATGCATATATGCAGATGGATGGGGAACCATGGAAGCAGCCAATGCACGAGGAGTATTCAACGTTGGTGGAAATTACGAGGGTTCCGTTTCAATCACTGATGATTCATGGAGACAATCACTGA

Protein Analysis

462

Amino Acids

51.55

Weight (kDa)

6.19

Isoelectric Point (pI)

45.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18730 AT4G30340 AT4G30340 AT5G57690
fragaria_vesca FvH4_4g08400 FvH4_4g08400
malus_domestica MD13G1262800.v1.1 MD16G1264500.v1.1
prunus_persica Prupe.1G094900_v2.0.a1
pyrus_communis pycom13g24340 pycom16g23310
rosa_chinensis RchiOBHm_Chr4g0404101
rosa_laevigata RLG00000008925
rosa_multiflora Rmu_sc0006579.1_g000001
rosa_roxburghii Rroxscaffold_5G00348640
rosa_rugosa Rorug04G0046900 Rorug04G0047000
rosa_samantha Rh4DG115900
rosa_wichuraiana Rw4G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 197, 765
Acc36I ACCTGC 1 cut(s) 584
AccII CGCG 2 cut(s) 69, 205
AclI AACGTT 1 cut(s) 1325
AclWI GGATC 1 cut(s) 920
AcsI RAATTY 2 cut(s) 302, 1146
AcuI CTGAAG 3 cut(s) 705, 757, 1103
AfaI GTAC 6 cut(s) 50, 412, 426, 649, 1020, 1094
AfiI CCNNNNNNNGG 4 cut(s) 98, 115, 209, 479
AflII CTTAAG 1 cut(s) 955
AgsI TTSAA 6 cut(s) 676, 858, 1014, 1145, 1323, 1355
AjnI CCWGG 2 cut(s) 515, 928
AluBI AGCT 3 cut(s) 22, 34, 679
AluI AGCT 3 cut(s) 22, 34, 679
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 4 cut(s) 120, 698, 1204, 1368
AlwI GGATC 1 cut(s) 920
AoxI GGCC 4 cut(s) 224, 1119, 1135, 1153
ApeKI GCWGC 2 cut(s) 1226, 1300
ApoI RAATTY 2 cut(s) 302, 1146
Asp700I GAANNNNTTC 2 cut(s) 306, 468
AspLEI GCGC 2 cut(s) 69, 695
AspS9I GGNCC 5 cut(s) 88, 224, 596, 848, 1120
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 3 cut(s) 272, 362, 416
AvaII GGWCC 3 cut(s) 88, 596, 848
BanII GRGCYC 1 cut(s) 24
BarI GAAGNNNNNNTAC 4 cut(s) 41, 73, 1076, 1108
BauI CACGAG 1 cut(s) 1310
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 1213, 1312
BccI CCATC 6 cut(s) 250, 401, 1127, 1161, 1273, 1277
BceAI ACGGC 2 cut(s) 131, 591
BciT130I CCWGG 2 cut(s) 517, 930
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 4 cut(s) 120, 698, 1204, 1368
BfaI CTAG 1 cut(s) 329
BfmI CTRYAG 1 cut(s) 591
BfrI CTTAAG 1 cut(s) 955
BfuAI ACCTGC 1 cut(s) 584
BisI GCNGC 7 cut(s) 44, 109, 144, 170, 1224, 1227, 1301
BlsI GCNGC 7 cut(s) 45, 110, 145, 171, 1225, 1228, 1302
BmcAI AGTACT 1 cut(s) 1094
Bme1390I CCNGG 3 cut(s) 117, 517, 930
Bme18I GGWCC 3 cut(s) 88, 596, 848
BmgT120I GGNCC 5 cut(s) 88, 224, 596, 848, 1120
BmiI GGNNCC 6 cut(s) 90, 245, 465, 660, 1290, 1347
BmrFI CCNGG 3 cut(s) 117, 517, 930
BmsI GCATC 4 cut(s) 30, 778, 951, 1184
BpmI CTGGAG 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 1218
BpuEI CTTGAG 3 cut(s) 8, 218, 876
BpuMI CCSGG 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 183, 1070, 1292
BsaXI ACNNNNNCTCC 2 cut(s) 511, 541
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 115, 209, 479
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 3 cut(s) 467, 476, 712
Bse3DI GCAATG 1 cut(s) 1212
BseBI CCWGG 2 cut(s) 517, 930
BseDI CCNNGG 3 cut(s) 183, 1070, 1292
BseGI GGATG 4 cut(s) 793, 966, 1172, 1288
BseLI CCNNNNNNNGG 4 cut(s) 98, 115, 209, 479
BseMI GCAATG 1 cut(s) 1212
BseMII CTCAG 5 cut(s) 297, 723, 888, 999, 1232
BseNI ACTGG 3 cut(s) 467, 476, 712
BseRI GAGGAG 2 cut(s) 32, 1328
BseXI GCAGC 2 cut(s) 1213, 1312
Bsh1236I CGCG 2 cut(s) 69, 205
BshFI GGCC 4 cut(s) 226, 1121, 1137, 1155
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 3 cut(s) 99, 116, 173
BsiWI CGTACG 1 cut(s) 1018
BslFI GGGAC 1 cut(s) 954
BslI CCNNNNNNNGG 4 cut(s) 98, 115, 209, 479
BsmAI GTCTC 4 cut(s) 120, 698, 1204, 1368
BsmBI CGTCTC 1 cut(s) 1204
BsmFI GGGAC 1 cut(s) 954
BsmI GAATGC 1 cut(s) 695
BsnI GGCC 4 cut(s) 226, 1121, 1137, 1155
Bso31I GGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 24
Bsp143I GATC 3 cut(s) 381, 508, 925
Bsp19I CCATGG 2 cut(s) 183, 1292
BspANI GGCC 4 cut(s) 226, 1121, 1137, 1155
BspCNI CTCAG 5 cut(s) 296, 724, 887, 998, 1231
BspFNI CGCG 2 cut(s) 69, 205
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 6 cut(s) 90, 245, 465, 660, 1290, 1347
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 584
BspPI GGATC 1 cut(s) 920
BspTI CTTAAG 1 cut(s) 955
BspTNI GGTCTC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 1212
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 3 cut(s) 467, 476, 712
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 3 cut(s) 183, 1070, 1292
BssMI GATC 3 cut(s) 381, 508, 925
BssSI CACGAG 1 cut(s) 1310
BssT1I CCWWGG 2 cut(s) 183, 1292
Bst2BI CACGAG 1 cut(s) 1310
Bst2UI CCWGG 2 cut(s) 517, 930
Bst4CI ACNGT 3 cut(s) 81, 415, 834
BstAFI CTTAAG 1 cut(s) 955
BstAPI GCANNNNNTGC 3 cut(s) 628, 1031, 1306
BstC8I GCNNGC 3 cut(s) 100, 642, 1173
BstDEI CTNAG 5 cut(s) 283, 732, 874, 985, 1218
BstDSI CCRYGG 3 cut(s) 183, 1070, 1292
BstENI CCTNNNNNAGG 1 cut(s) 477
BstF5I GGATG 4 cut(s) 793, 966, 1172, 1288
BstFNI CGCG 2 cut(s) 69, 205
BstHHI GCGC 2 cut(s) 69, 695
BstKTI GATC 3 cut(s) 384, 511, 928
BstMAI GTCTC 4 cut(s) 120, 698, 1204, 1368
BstMBI GATC 3 cut(s) 381, 508, 925
BstNI CCWGG 2 cut(s) 517, 930
BstNSI RCATGY 2 cut(s) 968, 1175
BstSCI CCNGG 3 cut(s) 115, 515, 928
BstSFI CTRYAG 1 cut(s) 591
BstUI CGCG 2 cut(s) 69, 205
BstV1I GCAGC 2 cut(s) 1213, 1312
BsuRI GGCC 4 cut(s) 226, 1121, 1137, 1155
BtgI CCRYGG 3 cut(s) 183, 1070, 1292
BtsCI GGATG 4 cut(s) 793, 966, 1172, 1288
BtsIMutI CAGTG 3 cut(s) 1011, 1358, 1381
BveI ACCTGC 1 cut(s) 584
Cac8I GCNNGC 3 cut(s) 100, 642, 1173
CciI TCATGA 1 cut(s) 298
CfoI GCGC 2 cut(s) 69, 695
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 5 cut(s) 88, 224, 596, 848, 1120
Csp6I GTAC 6 cut(s) 49, 411, 425, 648, 1019, 1093
CspCI CAANNNNNGTGG 2 cut(s) 1311, 1346
CviAII CATG 8 cut(s) 184, 299, 552, 965, 1124, 1172, 1293, 1370
CviQI GTAC 6 cut(s) 49, 411, 425, 648, 1019, 1093
DdeI CTNAG 5 cut(s) 283, 732, 874, 985, 1218
DpnI GATC 3 cut(s) 383, 510, 927
DpnII GATC 3 cut(s) 381, 508, 925
Ecl136II GAGCTC 1 cut(s) 22
Eco130I CCWWGG 2 cut(s) 183, 1292
Eco24I GRGCYC 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 120
Eco47I GGWCC 3 cut(s) 88, 596, 848
Eco53kI GAGCTC 1 cut(s) 22
Eco57I CTGAAG 3 cut(s) 705, 757, 1103
EcoICRI GAGCTC 1 cut(s) 22
EcoNI CCTNNNNNAGG 1 cut(s) 477
EcoO109I RGGNCCY 1 cut(s) 88
EcoRII CCWGG 2 cut(s) 515, 928
EcoT14I CCWWGG 2 cut(s) 183, 1292
EcoT22I ATGCAT 3 cut(s) 966, 1177, 1270
EcoT38I GRGCYC 1 cut(s) 24
ErhI CCWWGG 2 cut(s) 183, 1292
Esp3I CGTCTC 1 cut(s) 1204
FaeI CATG 8 cut(s) 187, 302, 555, 968, 1127, 1175, 1296, 1373
FalI AAGNNNNNCTT 6 cut(s) 20, 52, 938, 970, 1094, 1126
FaqI GGGAC 1 cut(s) 954
FatI CATG 8 cut(s) 183, 298, 551, 964, 1123, 1171, 1292, 1369
FauI CCCGC 1 cut(s) 637
Fnu4HI GCNGC 7 cut(s) 44, 109, 144, 170, 1224, 1227, 1301
FokI GGATG 4 cut(s) 800, 973, 1179, 1295
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 7 cut(s) 44, 109, 144, 170, 1224, 1227, 1301
FspBI CTAG 1 cut(s) 329
GlaI GCGC 2 cut(s) 68, 694
GluI GCNGC 7 cut(s) 44, 109, 144, 170, 1224, 1227, 1301
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 4 cut(s) 226, 1121, 1137, 1155
HapII CCGG 3 cut(s) 99, 116, 173
HhaI GCGC 2 cut(s) 69, 695
Hin1II CATG 8 cut(s) 187, 302, 555, 968, 1127, 1175, 1296, 1373
Hin6I GCGC 2 cut(s) 67, 693
HinP1I GCGC 2 cut(s) 67, 693
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 4 cut(s) 71, 176, 807, 1366
HpaII CCGG 3 cut(s) 99, 116, 173
HphI GGTGA 3 cut(s) 272, 362, 416
Hpy166II GTNNAC 1 cut(s) 871
Hpy188I TCNGA 3 cut(s) 286, 988, 1083
Hpy188III TCNNGA 7 cut(s) 25, 37, 125, 299, 329, 698, 1142
Hpy8I GTNNAC 1 cut(s) 871
Hpy99I CGWCG 2 cut(s) 137, 152
HpyAV CCTTC 4 cut(s) 122, 746, 1020, 1148
HpyCH4III ACNGT 3 cut(s) 81, 415, 834
HpyCH4IV ACGT 2 cut(s) 354, 1325
HpyF3I CTNAG 5 cut(s) 283, 732, 874, 985, 1218
HpySE526I ACGT 2 cut(s) 354, 1325
Hsp92II CATG 8 cut(s) 187, 302, 555, 968, 1127, 1175, 1296, 1373
HspAI GCGC 2 cut(s) 67, 693
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Kzo9I GATC 3 cut(s) 381, 508, 925
LmnI GCTCC 4 cut(s) 19, 249, 658, 994
Lsp1109I GCAGC 2 cut(s) 1213, 1312
LweI GCATC 4 cut(s) 30, 778, 951, 1184
MaeI CTAG 1 cut(s) 329
MaeII ACGT 2 cut(s) 354, 1325
MaeIII GTNAC 1 cut(s) 350
MalI GATC 3 cut(s) 383, 510, 927
MboI GATC 3 cut(s) 381, 508, 925
MboII GAAGA 7 cut(s) 40, 228, 275, 698, 895, 1034, 1074
MfeI CAATTG 2 cut(s) 248, 1026
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 9 cut(s) 248, 302, 766, 852, 979, 1026, 1146, 1230, 1335
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 1218
Mph1103I ATGCAT 3 cut(s) 966, 1177, 1270
MroNI GCCGGC 1 cut(s) 98
MroXI GAANNNNTTC 2 cut(s) 306, 468
MseI TTAA 4 cut(s) 83, 567, 935, 956
MslI CAYNNNNRTG 4 cut(s) 188, 782, 1050, 1128
MspA1I CMGCKG 1 cut(s) 644
MspCI CTTAAG 1 cut(s) 955
MspI CCGG 3 cut(s) 99, 116, 173
MspR9I CCNGG 3 cut(s) 117, 517, 930
MunI CAATTG 2 cut(s) 248, 1026
Mva1269I GAATGC 1 cut(s) 695
MvaI CCWGG 2 cut(s) 517, 930
MvnI CGCG 2 cut(s) 69, 205
NaeI GCCGGC 1 cut(s) 100
NciI CCSGG 1 cut(s) 117
NcoI CCATGG 2 cut(s) 183, 1292
NdeII GATC 3 cut(s) 381, 508, 925
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 8 cut(s) 187, 302, 555, 968, 1127, 1175, 1296, 1373
NlaIV GGNNCC 6 cut(s) 90, 245, 465, 660, 1290, 1347
NmuCI GTSAC 1 cut(s) 350
NsiI ATGCAT 3 cut(s) 966, 1177, 1270
NspI RCATGY 2 cut(s) 968, 1175
PaeI GCATGC 1 cut(s) 1175
PagI TCATGA 1 cut(s) 298
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 1346
PctI GAATGC 1 cut(s) 695
PdiI GCCGGC 1 cut(s) 100
PdmI GAANNNNTTC 2 cut(s) 306, 468
PfeI GAWTC 3 cut(s) 71, 807, 1366
Pfl23II CGTACG 1 cut(s) 1018
PflFI GACNNNGTC 1 cut(s) 131
PfoI TCCNGGA 2 cut(s) 115, 515
PkrI GCNGC 7 cut(s) 45, 110, 145, 171, 1225, 1228, 1302
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 88
PsiI TTATAA 2 cut(s) 197, 765
Psp124BI GAGCTC 1 cut(s) 24
Psp1406I AACGTT 1 cut(s) 1325
Psp5II RGGWCCY 1 cut(s) 88
Psp6I CCWGG 2 cut(s) 515, 928
PspGI CCWGG 2 cut(s) 515, 928
PspLI CGTACG 1 cut(s) 1018
PspN4I GGNNCC 6 cut(s) 90, 245, 465, 660, 1290, 1347
PspPI GGNCC 5 cut(s) 88, 224, 596, 848, 1120
PspPPI RGGWCCY 1 cut(s) 88
PstI CTGCAG 1 cut(s) 595
PsyI GACNNNGTC 1 cut(s) 131
RsaI GTAC 6 cut(s) 50, 412, 426, 649, 1020, 1094
RsaNI GTAC 6 cut(s) 49, 411, 425, 648, 1019, 1093
RseI CAYNNNNRTG 4 cut(s) 188, 782, 1050, 1128
SacI GAGCTC 1 cut(s) 24
SaqAI TTAA 4 cut(s) 83, 567, 935, 956
SatI GCNGC 7 cut(s) 44, 109, 144, 170, 1224, 1227, 1301
Sau3AI GATC 3 cut(s) 381, 508, 925
Sau96I GGNCC 5 cut(s) 88, 224, 596, 848, 1120
ScaI AGTACT 1 cut(s) 1094
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 3 cut(s) 117, 517, 930
SduI GDGCHC 1 cut(s) 24
SfaNI GCATC 4 cut(s) 30, 778, 951, 1184
SfcI CTRYAG 1 cut(s) 591
SinI GGWCC 3 cut(s) 88, 596, 848
SmiMI CAYNNNNRTG 4 cut(s) 188, 782, 1050, 1128
SmlI CTYRAG 4 cut(s) 23, 233, 891, 955
SmoI CTYRAG 4 cut(s) 23, 233, 891, 955
SphI GCATGC 1 cut(s) 1175
Sse9I AATT 9 cut(s) 248, 302, 766, 852, 979, 1026, 1146, 1230, 1335
SspMI CTAG 1 cut(s) 329
SstI GAGCTC 1 cut(s) 24
StyD4I CCNGG 3 cut(s) 115, 515, 928
StyI CCWWGG 2 cut(s) 183, 1292
TaaI ACNGT 3 cut(s) 81, 415, 834
TaiI ACGT 2 cut(s) 357, 1328
TaqI TCGA 2 cut(s) 124, 150
TasI AATT 9 cut(s) 248, 302, 766, 852, 979, 1026, 1146, 1230, 1335
TatI WGTACW 1 cut(s) 1092
TauI GCSGC 5 cut(s) 46, 111, 146, 172, 1226
TfiI GAWTC 3 cut(s) 71, 807, 1366
Tru1I TTAA 4 cut(s) 83, 567, 935, 956
Tru9I TTAA 4 cut(s) 83, 567, 935, 956
TscAI CASTG 2 cut(s) 1011, 1365
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 2 cut(s) 1226, 1300
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 5 cut(s) 315, 456, 528, 1034, 1358
TspGWI ACGGA 2 cut(s) 1059, 1338
TspRI CASTG 2 cut(s) 1011, 1365
Tth111I GACNNNGTC 1 cut(s) 131
Vha464I CTTAAG 1 cut(s) 955
VpaK11BI GGWCC 3 cut(s) 88, 596, 848
XagI CCTNNNNNAGG 1 cut(s) 477
XapI RAATTY 2 cut(s) 302, 1146
XbaI TCTAGA 1 cut(s) 328
XceI RCATGY 2 cut(s) 968, 1175
XcmI CCANNNNNNNNNTGG 2 cut(s) 800, 1130
XmnI GAANNNNTTC 2 cut(s) 306, 468
XspI CTAG 1 cut(s) 329
ZrmI AGTACT 1 cut(s) 1094
Zsp2I ATGCAT 3 cut(s) 966, 1177, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.