Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0011984

Physical Location & Seq

Genomic Coordinates Reverse (-)

20972805 .. 20980323

Gene Structure 7519 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00348640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGGAATCTCCGTCGTCATCGGCGACGCGCGTGACGGCGCGATCGTCGGTGATGGACTCGATCAGGGGGTGCAGCCTATCGGGGATTCGGATAGACAAGGAGGATTTGAAGAAGAAGCTGCTGATGCCGACTTACATACGGTTCGCCATGAGGGATTCGATAAGGTTGAAGGACCGGACCGCCGGAGAGAGCCGGCTTCCCGGAAGCAGAGACCTCGTCGCCGTTCCCTCCGACGGCAATGGCGAGGAGTCGTCGGCGCCGGAGATGCCTATGGTTGTGTTTATTAATCCCAAGAGCGGTGGCAGACACGGACCTATGCTTAAGGAGAGGCTCCAGCTGCTCATGGGCGAAGAGCAGGTTTTTGACCTCTCAGACGTGAAGCCTCATGAATTTGTTCAGTATGGATTGGGTTGTCTTGAGCGGTTTGCGGAACTTGGTGACAAGTGTGCAAAAGAGTGTCGTCAAAAGATCAGGGTTATGGTTGCAGGGGGTGATGGGACAGTTGGTTGGGTGCTAGGTTGTCTATCTGAGCTTCAGAAGCAGGGCAGGGAACCAGTTCCTCCAGTAGGAATTATTCCACTTGGAACGGGAAATGATCTATCTAGGAGTTTTGGTTGGGGTGGCTCATTCCCTTTCGCCTGGAAATCAGCCATAAAAAAAACTCTTTTCAGAGCTACTACAGGTCCTCTTTGTCGTTTGGATAGTTGGAATATTCTAATACAAATGCCAGATGGTGCAGTTTTGGAACCACCTCATTCTTTGAAGCTTGCCGACGAGTGCACTCTTGATGAGGTTGTAAATCCGGGTTTGGAAATTCAAGGGCGGTTGCCTGAGAAACGGACTTGCTACGAAGGAGTGTTTTATAATTACTTTAGCATAGGGATGGATGCCAAAGTAGCTTATGGATTCCACCATCTAAGAAACGAGAAACCCTATCTTGCACAAGGTCCAATTTCAAATAAGTTGGTTTACTCTGGTTACAGTTGCACACAAGGCTGGTTCTTCACACCTTGCACAAGTAATCCAAGTTTAAGGGGACTAAAGAATATCCTAAGGATGCATGTTAAAAAGGTTAATTGCTCAGAATGGGAGCAGATTCCAGTGCCGTCAAGTGTAAGGGCAATCGTTGCTCTGAATCTTCATAACTATGGGAGCGGAAGAAACCCATGGGGTAATCTGAAGCCTGATTACTTGGAAAAGAAAGGCTTTGTGGAGGCCCATGCTGATGATGGTCTTCTAGAAATTTTTGGCCTGAAACAAGGATGGCATGCATCATTTGTTATGGTTGAACTCATCTCTGCCAAACATATTGCCCAGGCTGCTGCAATTCGGTTGGAAGTTAGAGGTGGGGAATGGAGAAATGCATATATGCAGATGGATGGGGAACCATGGAAGCAGCCAATGGACGAGGAATTTTCAACGCTTGTGGAAATTAAGAGGGTTCCTTATCAATCACCGATGATTAGTGGAGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004143 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0022622 GO:0030054 GO:0032501 GO:0032502 GO:0044237 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055044 GO:0071944 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

491

Amino Acids

54.62

Weight (kDa)

8.19

Isoelectric Point (pI)

46.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	91 - 224	4.9e-24	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	286 - 463	1.7e-42	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011984)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18730 AT4G30340 AT4G30340 AT5G57690
fragaria_vesca	FvH4_4g08400 FvH4_4g08400
malus_domestica	MD13G1262800.v1.1 MD16G1264500.v1.1
prunus_persica	Prupe.1G094900_v2.0.a1
pyrus_communis	pycom13g24340 pycom16g23310
rosa_chinensis	RchiOBHm_Chr4g0404101
rosa_laevigata	RLG00000008925
rosa_multiflora	Rmu_sc0006579.1_g000001
rosa_roxburghii	Rroxscaffold_5G00348640
rosa_rugosa	Rorug04G0046900 Rorug04G0047000
rosa_samantha	Rh4DG115900
rosa_wichuraiana	Rw4G009980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	864
Acc36I	ACCTGC	1 cut(s)	346
AccB1I	GGYRCC	1 cut(s)	256
AccBSI	CCGCTC	3 cut(s)	297, 421, 1155
AccII	CGCG	3 cut(s)	28, 30, 40
AciI	CCGC	6 cut(s)	180, 297, 421, 428, 823, 1155
AcsI	RAATTY	4 cut(s)	389, 813, 1242, 1412
AcuI	CTGAAG	2 cut(s)	518, 1199
AcyI	GRCGYC	1 cut(s)	257
AfiI	CCNNNNNNNGG	3 cut(s)	233, 296, 566
AflII	CTTAAG	1 cut(s)	320
AgsI	TTSAA	7 cut(s)	109, 169, 763, 818, 957, 1289, 1419
AjiI	CACGTC	1 cut(s)	376
AjnI	CCWGG	2 cut(s)	638, 1314
AluBI	AGCT	6 cut(s)	118, 337, 532, 674, 766, 899
AluI	AGCT	6 cut(s)	118, 337, 532, 674, 766, 899
Alw21I	GWGCWC	1 cut(s)	782
Alw26I	GTCTC	2 cut(s)	204, 1464
Alw44I	GTGCAC	1 cut(s)	778
AoxI	GGCC	2 cut(s)	1215, 1249
ApaLI	GTGCAC	1 cut(s)	778
ApeKI	GCWGC	6 cut(s)	72, 118, 337, 1319, 1322, 1396
ApoI	RAATTY	4 cut(s)	389, 813, 1242, 1412
AseI	ATTAAT	1 cut(s)	285
Asp700I	GAANNNNTTC	2 cut(s)	393, 555
AspLEI	GCGC	3 cut(s)	30, 40, 259
AspS9I	GGNCC	6 cut(s)	172, 177, 311, 683, 947, 1216
AsuC2I	CCSGG	2 cut(s)	201, 804
AsuHPI	GGTGA	4 cut(s)	61, 449, 503, 1446
AvaII	GGWCC	5 cut(s)	172, 177, 311, 683, 947
AxyI	CCTNAGG	1 cut(s)	1052
BaeGI	GKGCMC	1 cut(s)	782
BanI	GGYRCC	1 cut(s)	256
BarI	GAAGNNNNNNTAC	2 cut(s)	1172, 1204
BbsI	GAAGAC	1 cut(s)	1226
Bbv12I	GWGCWC	1 cut(s)	782
BbvI	GCAGC	6 cut(s)	84, 105, 324, 1306, 1309, 1408
BccI	CCATC	9 cut(s)	46, 488, 725, 877, 921, 1223, 1257, 1369, 1373
BceAI	ACGGC	4 cut(s)	51, 206, 250, 1090
BcgI	CGANNNNNNTGC	2 cut(s)	196, 230
BciT130I	CCWGG	2 cut(s)	640, 1316
BcnI	CCSGG	2 cut(s)	201, 804
BcoDI	GTCTC	2 cut(s)	204, 1464
BfaI	CTAG	3 cut(s)	515, 603, 1238
BfmI	CTRYAG	1 cut(s)	678
BfoI	RGCGCY	1 cut(s)	260
BfrI	CTTAAG	1 cut(s)	320
BfuAI	ACCTGC	1 cut(s)	346
BisI	GCNGC	6 cut(s)	73, 119, 338, 1320, 1323, 1397
BlsI	GCNGC	6 cut(s)	74, 120, 339, 1321, 1324, 1398
Bme1390I	CCNGG	4 cut(s)	201, 640, 804, 1316
Bme18I	GGWCC	5 cut(s)	172, 177, 311, 683, 947
BmgBI	CACGTC	1 cut(s)	376
BmgT120I	GGNCC	6 cut(s)	172, 177, 311, 683, 947, 1216
BmiI	GGNNCC	6 cut(s)	258, 332, 552, 747, 1386, 1443
BmrFI	CCNGG	4 cut(s)	201, 640, 804, 1316
BmsI	GCATC	5 cut(s)	114, 255, 877, 1047, 1280
BpiI	GAAGAC	1 cut(s)	1226
BpmI	CTGGAG	2 cut(s)	317, 546
BpuEI	CTTGAG	1 cut(s)	437
BpuMI	CCSGG	2 cut(s)	201, 804
BsaHI	GRCGYC	1 cut(s)	257
BsaI	GGTCTC	1 cut(s)	204
BsaJI	CCNNGG	3 cut(s)	1166, 1314, 1388
BsaWI	WCCGGW	1 cut(s)	174
BsaXI	ACNNNNNCTCC	2 cut(s)	598, 628
Bsc4I	CCNNNNNNNGG	3 cut(s)	233, 296, 566
Bse118I	RCCGGY	1 cut(s)	192
Bse1I	ACTGG	3 cut(s)	554, 563, 1100
Bse21I	CCTNAGG	1 cut(s)	1052
Bse3DI	GCAATG	1 cut(s)	244
BseBI	CCWGG	2 cut(s)	640, 1316
BseDI	CCNNGG	3 cut(s)	1166, 1314, 1388
BseGI	GGATG	5 cut(s)	888, 892, 1062, 1268, 1384
BseLI	CCNNNNNNNGG	3 cut(s)	233, 296, 566
BseMI	GCAATG	1 cut(s)	244
BseMII	CTCAG	4 cut(s)	384, 519, 822, 1095
BseNI	ACTGG	3 cut(s)	554, 563, 1100
BseRI	GAGGAG	1 cut(s)	260
BseSI	GKGCMC	1 cut(s)	782
BseXI	GCAGC	6 cut(s)	84, 105, 324, 1306, 1309, 1408
BsgI	GTGCAG	2 cut(s)	91, 756
Bsh1236I	CGCG	3 cut(s)	28, 30, 40
Bsh1285I	CGRYCG	1 cut(s)	44
BshFI	GGCC	2 cut(s)	1217, 1251
BshNI	GGYRCC	1 cut(s)	256
BsiEI	CGRYCG	1 cut(s)	44
BsiHKAI	GWGCWC	1 cut(s)	782
BsiSI	CCGG	6 cut(s)	175, 183, 193, 201, 260, 803
BslFI	GGGAC	2 cut(s)	511, 1050
BslI	CCNNNNNNNGG	3 cut(s)	233, 296, 566
BsmAI	GTCTC	2 cut(s)	204, 1464
BsmFI	GGGAC	2 cut(s)	511, 1050
BsnI	GGCC	2 cut(s)	1217, 1251
Bso31I	GGTCTC	1 cut(s)	204
Bsp1286I	GDGCHC	1 cut(s)	782
Bsp143I	GATC	4 cut(s)	41, 60, 468, 595
Bsp19I	CCATGG	2 cut(s)	1166, 1388
BspACI	CCGC	6 cut(s)	180, 297, 421, 428, 823, 1155
BspANI	GGCC	2 cut(s)	1217, 1251
BspCNI	CTCAG	4 cut(s)	383, 520, 823, 1094
BspFNI	CGCG	3 cut(s)	28, 30, 40
BspHI	TCATGA	1 cut(s)	385
BspLI	GGNNCC	6 cut(s)	258, 332, 552, 747, 1386, 1443
BspMI	ACCTGC	1 cut(s)	346
BspQI	GCTCTTC	1 cut(s)	345
BspT107I	GGYRCC	1 cut(s)	256
BspTI	CTTAAG	1 cut(s)	320
BspTNI	GGTCTC	1 cut(s)	204
BsrBI	CCGCTC	3 cut(s)	297, 421, 1155
BsrDI	GCAATG	1 cut(s)	244
BsrFI	RCCGGY	1 cut(s)	192
BsrI	ACTGG	3 cut(s)	554, 563, 1100
BssAI	RCCGGY	1 cut(s)	192
BssECI	CCNNGG	3 cut(s)	1166, 1314, 1388
BssMI	GATC	4 cut(s)	41, 60, 468, 595
BssNI	GRCGYC	1 cut(s)	257
BssT1I	CCWWGG	2 cut(s)	1166, 1388
Bst2UI	CCWGG	2 cut(s)	640, 1316
Bst4CI	ACNGT	3 cut(s)	141, 502, 983
Bst6I	CTCTTC	1 cut(s)	345
BstACI	GRCGYC	1 cut(s)	257
BstAFI	CTTAAG	1 cut(s)	320
BstAPI	GCANNNNNTGC	1 cut(s)	1127
BstC8I	GCNNGC	3 cut(s)	194, 768, 1269
BstDEI	CTNAG	6 cut(s)	370, 528, 831, 917, 1052, 1081
BstDSI	CCRYGG	2 cut(s)	1166, 1388
BstENI	CCTNNNNNAGG	1 cut(s)	564
BstF5I	GGATG	5 cut(s)	888, 892, 1062, 1268, 1384
BstFNI	CGCG	3 cut(s)	28, 30, 40
BstH2I	RGCGCY	1 cut(s)	260
BstHHI	GCGC	3 cut(s)	30, 40, 259
BstKTI	GATC	4 cut(s)	44, 63, 471, 598
BstMAI	GTCTC	2 cut(s)	204, 1464
BstMBI	GATC	4 cut(s)	41, 60, 468, 595
BstMCI	CGRYCG	1 cut(s)	44
BstMWI	GCNNNNNNNGC	8 cut(s)	124, 265, 337, 538, 896, 993, 1127, 1319
BstNI	CCWGG	2 cut(s)	640, 1316
BstNSI	RCATGY	2 cut(s)	1064, 1271
BstSCI	CCNGG	4 cut(s)	199, 638, 802, 1314
BstSFI	CTRYAG	1 cut(s)	678
BstSLI	GKGCMC	1 cut(s)	782
BstUI	CGCG	3 cut(s)	28, 30, 40
BstV1I	GCAGC	6 cut(s)	84, 105, 324, 1306, 1309, 1408
BstV2I	GAAGAC	1 cut(s)	1226
Bsu36I	CCTNAGG	1 cut(s)	1052
BsuRI	GGCC	2 cut(s)	1217, 1251
BtgI	CCRYGG	2 cut(s)	1166, 1388
BtrI	CACGTC	1 cut(s)	376
BtsCI	GGATG	5 cut(s)	888, 892, 1062, 1268, 1384
BtsIMutI	CAGTG	1 cut(s)	1107
BveI	ACCTGC	1 cut(s)	346
Cac8I	GCNNGC	3 cut(s)	194, 768, 1269
CciI	TCATGA	1 cut(s)	385
CfoI	GCGC	3 cut(s)	30, 40, 259
Cfr10I	RCCGGY	1 cut(s)	192
Cfr13I	GGNCC	6 cut(s)	172, 177, 311, 683, 947, 1216
CpoI	CGGWCCG	1 cut(s)	177
CseI	GACGC	1 cut(s)	34
CspCI	CAANNNNNGTGG	4 cut(s)	280, 315, 1407, 1442
CspI	CGGWCCG	1 cut(s)	177
CviAII	CATG	8 cut(s)	148, 343, 386, 1061, 1167, 1220, 1268, 1389
DdeI	CTNAG	6 cut(s)	370, 528, 831, 917, 1052, 1081
DinI	GGCGCC	1 cut(s)	258
DpnI	GATC	4 cut(s)	43, 62, 470, 597
DpnII	GATC	4 cut(s)	41, 60, 468, 595
Eam1104I	CTCTTC	1 cut(s)	345
EarI	CTCTTC	1 cut(s)	345
Eco130I	CCWWGG	2 cut(s)	1166, 1388
Eco31I	GGTCTC	1 cut(s)	204
Eco47I	GGWCC	5 cut(s)	172, 177, 311, 683, 947
Eco57I	CTGAAG	2 cut(s)	518, 1199
Eco81I	CCTNAGG	1 cut(s)	1052
EcoNI	CCTNNNNNAGG	1 cut(s)	564
EcoO109I	RGGNCCY	1 cut(s)	683
EcoRII	CCWGG	2 cut(s)	638, 1314
EcoT14I	CCWWGG	2 cut(s)	1166, 1388
EcoT22I	ATGCAT	3 cut(s)	1062, 1273, 1366
EgeI	GGCGCC	1 cut(s)	258
EheI	GGCGCC	1 cut(s)	258
ErhI	CCWWGG	2 cut(s)	1166, 1388
FaeI	CATG	8 cut(s)	151, 346, 389, 1064, 1170, 1223, 1271, 1392
FalI	AAGNNNNNCTT	2 cut(s)	1190, 1222
FaqI	GGGAC	2 cut(s)	511, 1050
FatI	CATG	8 cut(s)	147, 342, 385, 1060, 1166, 1219, 1267, 1388
Fnu4HI	GCNGC	6 cut(s)	73, 119, 338, 1320, 1323, 1397
FokI	GGATG	5 cut(s)	895, 899, 1069, 1275, 1391
Fsp4HI	GCNGC	6 cut(s)	73, 119, 338, 1320, 1323, 1397
FspBI	CTAG	3 cut(s)	515, 603, 1238
GlaI	GCGC	3 cut(s)	29, 39, 258
GluI	GCNGC	6 cut(s)	73, 119, 338, 1320, 1323, 1397
GsuI	CTGGAG	2 cut(s)	317, 546
HaeII	RGCGCY	1 cut(s)	260
HaeIII	GGCC	2 cut(s)	1217, 1251
HapII	CCGG	6 cut(s)	175, 183, 193, 201, 260, 803
HgaI	GACGC	1 cut(s)	34
HhaI	GCGC	3 cut(s)	30, 40, 259
Hin1I	GRCGYC	1 cut(s)	257
Hin1II	CATG	8 cut(s)	151, 346, 389, 1064, 1170, 1223, 1271, 1392
Hin6I	GCGC	3 cut(s)	28, 38, 257
HinP1I	GCGC	3 cut(s)	28, 38, 257
HindIII	AAGCTT	1 cut(s)	764
HinfI	GANTC	8 cut(s)	5, 56, 85, 155, 248, 906, 1096, 1135
HpaII	CCGG	6 cut(s)	175, 183, 193, 201, 260, 803
HphI	GGTGA	4 cut(s)	61, 449, 503, 1446
Hpy166II	GTNNAC	2 cut(s)	780, 970
Hpy188I	TCNGA	9 cut(s)	90, 232, 373, 529, 537, 671, 1084, 1134, 1179
Hpy188III	TCNNGA	4 cut(s)	386, 416, 785, 1238
Hpy8I	GTNNAC	2 cut(s)	780, 970
Hpy99I	CGWCG	7 cut(s)	16, 28, 49, 221, 236, 256, 776
HpyAV	CCTTC	2 cut(s)	163, 845
HpyCH4III	ACNGT	3 cut(s)	141, 502, 983
HpyCH4IV	ACGT	1 cut(s)	375
HpyF10VI	GCNNNNNNNGC	8 cut(s)	124, 265, 337, 538, 896, 993, 1127, 1319
HpyF3I	CTNAG	6 cut(s)	370, 528, 831, 917, 1052, 1081
HpySE526I	ACGT	1 cut(s)	375
Hsp92I	GRCGYC	1 cut(s)	257
Hsp92II	CATG	8 cut(s)	151, 346, 389, 1064, 1170, 1223, 1271, 1392
HspAI	GCGC	3 cut(s)	28, 38, 257
KasI	GGCGCC	1 cut(s)	256
KroI	GCCGGC	1 cut(s)	192
KroNI	GCCGGC	1 cut(s)	194
Kzo9I	GATC	4 cut(s)	41, 60, 468, 595
LguI	GCTCTTC	1 cut(s)	345
LmnI	GCTCC	3 cut(s)	336, 1090, 1152
Lsp1109I	GCAGC	6 cut(s)	84, 105, 324, 1306, 1309, 1408
LweI	GCATC	5 cut(s)	114, 255, 877, 1047, 1280
MaeI	CTAG	3 cut(s)	515, 603, 1238
MaeII	ACGT	1 cut(s)	375
MaeIII	GTNAC	3 cut(s)	31, 437, 977
MalI	GATC	4 cut(s)	43, 62, 470, 597
MbiI	CCGCTC	3 cut(s)	297, 421, 1155
MboI	GATC	4 cut(s)	41, 60, 468, 595
MboII	GAAGA	7 cut(s)	121, 124, 362, 994, 1130, 1170, 1226
MhlI	GDGCHC	1 cut(s)	782
Mly113I	GGCGCC	1 cut(s)	257
MlyI	GAGTC	2 cut(s)	50, 257
MmeI	TCCRAC	3 cut(s)	255, 686, 1314
Mph1103I	ATGCAT	3 cut(s)	1062, 1273, 1366
MroNI	GCCGGC	1 cut(s)	192
MroXI	GAANNNNTTC	2 cut(s)	393, 555
MseI	TTAA	6 cut(s)	285, 321, 1031, 1065, 1074, 1434
MslI	CAYNNNNRTG	3 cut(s)	881, 1146, 1224
MspA1I	CMGCKG	1 cut(s)	337
MspCI	CTTAAG	1 cut(s)	320
MspI	CCGG	6 cut(s)	175, 183, 193, 201, 260, 803
MspR9I	CCNGG	4 cut(s)	201, 640, 804, 1316
MvaI	CCWGG	2 cut(s)	640, 1316
MvnI	CGCG	3 cut(s)	28, 30, 40
MwoI	GCNNNNNNNGC	8 cut(s)	124, 265, 337, 538, 896, 993, 1127, 1319
NaeI	GCCGGC	1 cut(s)	194
NarI	GGCGCC	1 cut(s)	257
NciI	CCSGG	2 cut(s)	201, 804
NcoI	CCATGG	2 cut(s)	1166, 1388
NdeII	GATC	4 cut(s)	41, 60, 468, 595
NgoMIV	GCCGGC	1 cut(s)	192
NlaIII	CATG	8 cut(s)	151, 346, 389, 1064, 1170, 1223, 1271, 1392
NlaIV	GGNNCC	6 cut(s)	258, 332, 552, 747, 1386, 1443
NmuCI	GTSAC	2 cut(s)	31, 437
NsiI	ATGCAT	3 cut(s)	1062, 1273, 1366
NspI	RCATGY	2 cut(s)	1064, 1271
PaeI	GCATGC	1 cut(s)	1271
PagI	TCATGA	1 cut(s)	385
PciSI	GCTCTTC	1 cut(s)	345
PcsI	WCGNNNNNNNCGW	3 cut(s)	20, 41, 240
PdiI	GCCGGC	1 cut(s)	194
PdmI	GAANNNNTTC	2 cut(s)	393, 555
PfeI	GAWTC	6 cut(s)	5, 85, 155, 906, 1096, 1135
PflFI	GACNNNGTC	1 cut(s)	215
PfoI	TCCNGGA	1 cut(s)	199
PkrI	GCNGC	6 cut(s)	74, 120, 339, 1321, 1324, 1398
Ple19I	CGATCG	1 cut(s)	44
PleI	GAGTC	2 cut(s)	50, 256
PluTI	GGCGCC	1 cut(s)	260
PpsI	GAGTC	2 cut(s)	50, 256
PpuMI	RGGWCCY	1 cut(s)	683
PshBI	ATTAAT	1 cut(s)	285
PsiI	TTATAA	1 cut(s)	864
Psp5II	RGGWCCY	1 cut(s)	683
Psp6I	CCWGG	2 cut(s)	638, 1314
PspGI	CCWGG	2 cut(s)	638, 1314
PspN4I	GGNNCC	6 cut(s)	258, 332, 552, 747, 1386, 1443
PspPI	GGNCC	6 cut(s)	172, 177, 311, 683, 947, 1216
PspPPI	RGGWCCY	1 cut(s)	683
PsyI	GACNNNGTC	1 cut(s)	215
PvuI	CGATCG	1 cut(s)	44
PvuII	CAGCTG	1 cut(s)	337
RseI	CAYNNNNRTG	3 cut(s)	881, 1146, 1224
Rsr2I	CGGWCCG	1 cut(s)	177
RsrII	CGGWCCG	1 cut(s)	177
SapI	GCTCTTC	1 cut(s)	345
SaqAI	TTAA	6 cut(s)	285, 321, 1031, 1065, 1074, 1434
SatI	GCNGC	6 cut(s)	73, 119, 338, 1320, 1323, 1397
Sau3AI	GATC	4 cut(s)	41, 60, 468, 595
Sau96I	GGNCC	6 cut(s)	172, 177, 311, 683, 947, 1216
SchI	GAGTC	2 cut(s)	50, 257
ScrFI	CCNGG	4 cut(s)	201, 640, 804, 1316
SduI	GDGCHC	1 cut(s)	782
SfaNI	GCATC	5 cut(s)	114, 255, 877, 1047, 1280
SfcI	CTRYAG	1 cut(s)	678
SfoI	GGCGCC	1 cut(s)	258
SinI	GGWCC	5 cut(s)	172, 177, 311, 683, 947
SmiMI	CAYNNNNRTG	3 cut(s)	881, 1146, 1224
SmlI	CTYRAG	2 cut(s)	320, 416
SmoI	CTYRAG	2 cut(s)	320, 416
SphI	GCATGC	1 cut(s)	1271
SsiI	CCGC	6 cut(s)	180, 297, 421, 428, 823, 1155
SspDI	GGCGCC	1 cut(s)	256
SspI	AATATT	1 cut(s)	712
SspMI	CTAG	3 cut(s)	515, 603, 1238
StyD4I	CCNGG	4 cut(s)	199, 638, 802, 1314
StyI	CCWWGG	2 cut(s)	1166, 1388
TaaI	ACNGT	3 cut(s)	141, 502, 983
TaiI	ACGT	1 cut(s)	378
TaqI	TCGA	2 cut(s)	59, 158
TfiI	GAWTC	6 cut(s)	5, 85, 155, 906, 1096, 1135
Tru1I	TTAA	6 cut(s)	285, 321, 1031, 1065, 1074, 1434
Tru9I	TTAA	6 cut(s)	285, 321, 1031, 1065, 1074, 1434
TscAI	CASTG	1 cut(s)	1107
TseFI	GTSAC	2 cut(s)	31, 437
TseI	GCWGC	6 cut(s)	72, 118, 337, 1319, 1322, 1396
Tsp45I	GTSAC	2 cut(s)	31, 437
TspDTI	ATGAA	2 cut(s)	402, 1130
TspGWI	ACGGA	2 cut(s)	324, 853
TspRI	CASTG	1 cut(s)	1107
Tth111I	GACNNNGTC	1 cut(s)	215
Vha464I	CTTAAG	1 cut(s)	320
VneI	GTGCAC	1 cut(s)	778
VpaK11BI	GGWCC	5 cut(s)	172, 177, 311, 683, 947
VspI	ATTAAT	1 cut(s)	285
XagI	CCTNNNNNAGG	1 cut(s)	564
XapI	RAATTY	4 cut(s)	389, 813, 1242, 1412
XbaI	TCTAGA	1 cut(s)	1237
XceI	RCATGY	2 cut(s)	1064, 1271
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1226
XmnI	GAANNNNTTC	2 cut(s)	393, 555
XspI	CTAG	3 cut(s)	515, 603, 1238
Zsp2I	ATGCAT	3 cut(s)	1062, 1273, 1366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.