AT5G57690

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011984
Physical Location & Seq
Reverse (-)
23371675 .. 23374676
3002 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57690.1

Sequence Viewer

Length: 1464 bp
ATGGAATCACCGTCGATTGGTGATTCATTGACGGCGAGGATGATTCCACGACATTCATCTCTCGACTCATTCGGAGCAATGAAAGTCTCTTTATTGGTCAATCTCGCGAGCATAAGAGTCTCTAAAGCAGAGCTTCGTCAAAGAGTTATGCTTCCTCAATATCTCCGCATTGCAATACGCGATTGCATTCTTCGTAAAGACGATTCCTTCGACGCTTCCTCCTCTGTAGCACCACCGTTAGAAAACAACGCGTTGACGCCTGAAGTTCCTTTGATGGTCTTCGTTAATCCCAAAAGCGGTGGTCGTCAAGGTCCACTCATTAAAGAACGTCTCCAGAATCTTATTAGCGAAGAACAGGTGTATGATTTAACGGAAGTGAAGCCTAATGAGTTTATTCGATATGGATTGGGATGCTTAGAGGCATTTGCGTCTCGAGGAGACGAATGTGCTAAAGAGATTCGTGAAAAGATGAGAATTGTGGTTGCAGGTGGAGATGGAACCGTTGGTTGGGTTCTAGGATGTCTCGGTGAACTTAATTTACAAAACCGGTTACCCGTTCCTCCGGTTTCGATAATGCCACTCGGAACGGGAAACGATCTTTCAAGAAGTTTCGGATGGGGAGGTTCATTCCCATTTGCGTGGAAATCTGCGATAAAACGAACACTTCATAGAGCAAGTGTTGCTCCTATAAGCCGTTTAGATAGTTGGAATATTCTGATTACAATGCCTTCTGGTGAAATTGTGGATCCTCCTTATTCTTTAAAGGCTACACAAGAATGTTACATTGACCAGAATTTGGAGATAGAGGGAGAGATCCCTCCATCGACGAATGGCTATGAAGGAGTCTTCTACAATTACTTTAGTATAGGTATGGATGCTCAAGTGGCTTACGGTTTTCACCATTTGCGCAACGAAAAGCCTTACCTTGCAAATGGCCCTATCGCCAATAAGATTATATACTCGGGATATGGTTGCTCTCAGGGTTGGTTCCTCACTCACTGCATAAATGACCCAGGTTTAAGGGGTCTCAAGAACATTATGACATTGCATATAAAAAAGTTGGACTCATCCGAGTGGGAGAAAGTACCAGTTCCCAAAAGTGTAAGAGCAGTTGTTGCATTGAATCTACATAGCTATGGAAGTGGAAGAAACCCTTGGGGAAATCTCAAACAAGATTATCTTGAAAAAAGAGGTTTTGTGGAGGCACAAGCCGATGATGGACTACTTGAGATCTTTGGGTTAAAGCAAGGATGGCATGCTTCCTTTGTTATGGTTGAGCTTATATCTGCCAAACACATTGCACAGGCGGCAGCGATAAGGCTGGAGATAAGAGGAGGAGACTGGAAAGATGCGTTTATGCAAATGGATGGAGAGCCATGGAAACAGCCTATGACCAGAGACTACTCTACCTTTGTTGACATTAAGAGAGTCCCTCACCAGTCTTTGGTCGTCAAAGGAGATTGA

Protein Analysis

487

Amino Acids

54.18

Weight (kDa)

7.14

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 90 - 228 5.9e-26 Diacylglycerol kinase catalytic domain
DAGK_acc PF00609 282 - 459 2.8e-44 Diacylglycerol kinase accessory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18730 AT4G30340 AT4G30340 AT5G57690
fragaria_vesca FvH4_4g08400 FvH4_4g08400
malus_domestica MD13G1262800.v1.1 MD16G1264500.v1.1
prunus_persica Prupe.1G094900_v2.0.a1
pyrus_communis pycom13g24340 pycom16g23310
rosa_chinensis RchiOBHm_Chr4g0404101
rosa_laevigata RLG00000008925
rosa_multiflora Rmu_sc0006579.1_g000001
rosa_roxburghii Rroxscaffold_5G00348640
rosa_rugosa Rorug04G0046900 Rorug04G0047000
rosa_samantha Rh4DG115900
rosa_wichuraiana Rw4G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
Acc16I TGCGCA 1 cut(s) 908
Acc36I ACCTGC 1 cut(s) 476
AccB7I CCANNNNNTGG 2 cut(s) 796, 1444
AccII CGCG 3 cut(s) 107, 180, 251
AciI CCGC 3 cut(s) 166, 297, 1307
AclWI GGATC 3 cut(s) 740, 753, 808
AcsI RAATTY 1 cut(s) 793
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 257
AfaI GTAC 1 cut(s) 1086
AfiI CCNNNNNNNGG 5 cut(s) 17, 296, 507, 796, 1444
AflIII ACRYGT 1 cut(s) 249
AgeI ACCGGT 1 cut(s) 546
AgsI TTSAA 3 cut(s) 603, 1123, 1184
AjnI CCWGG 1 cut(s) 1012
AjuI GAANNNNNNNTTGG 4 cut(s) 490, 522, 1138, 1170
AluBI AGCT 3 cut(s) 133, 1134, 1279
AluI AGCT 3 cut(s) 133, 1134, 1279
Alw26I GTCTC 9 cut(s) 91, 124, 335, 432, 435, 527, 1031, 1332, 1392
AlwI GGATC 3 cut(s) 740, 753, 808
Ama87I CYCGRG 2 cut(s) 432, 961
AoxI GGCC 1 cut(s) 934
ApeKI GCWGC 1 cut(s) 1310
ApoI RAATTY 1 cut(s) 793
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AsiGI ACCGGT 1 cut(s) 546
AspLEI GCGC 1 cut(s) 909
AspS9I GGNCC 2 cut(s) 311, 935
AsuHPI GGTGA 5 cut(s) 32, 539, 746, 890, 1427
AvaI CYCGRG 2 cut(s) 432, 961
AvaII GGWCC 1 cut(s) 311
BamHI GGATCC 1 cut(s) 745
BbsI GAAGAC 2 cut(s) 271, 838
BbvI GCAGC 1 cut(s) 1322
BccI CCATC 7 cut(s) 268, 488, 609, 829, 1211, 1245, 1361
BceAI ACGGC 2 cut(s) 48, 678
BciT130I CCWGG 1 cut(s) 1014
BcoDI GTCTC 9 cut(s) 91, 124, 335, 432, 435, 527, 1031, 1332, 1392
BfaI CTAG 1 cut(s) 515
BfmI CTRYAG 1 cut(s) 225
BfuAI ACCTGC 1 cut(s) 476
BglII AGATCT 1 cut(s) 1230
BisI GCNGC 2 cut(s) 1308, 1311
BlsI GCNGC 2 cut(s) 1309, 1312
Bme1390I CCNGG 1 cut(s) 1014
Bme18I GGWCC 1 cut(s) 311
BmeT110I CYCGRG 2 cut(s) 432, 961
BmgT120I GGNCC 2 cut(s) 311, 935
BmiI GGNNCC 3 cut(s) 499, 747, 989
BmrFI CCNGG 1 cut(s) 1014
BmsI GCATC 3 cut(s) 401, 865, 1339
BpiI GAAGAC 2 cut(s) 271, 838
BplI GAGNNNNNCTC 2 cut(s) 1417, 1449
BpmI CTGGAG 2 cut(s) 317, 1343
BpuEI CTTGAG 3 cut(s) 864, 1013, 1247
BsaHI GRCGYC 1 cut(s) 257
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 3 cut(s) 1012, 1154, 1376
BsaWI WCCGGW 2 cut(s) 546, 562
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 296, 507, 796, 1444
Bse118I RCCGGY 1 cut(s) 546
Bse1I ACTGG 3 cut(s) 1088, 1346, 1438
Bse3DI GCAATG 4 cut(s) 84, 168, 1043, 1296
BseBI CCWGG 1 cut(s) 1014
BseDI CCNNGG 3 cut(s) 1012, 1154, 1376
BseGI GGATG 8 cut(s) 45, 416, 524, 620, 880, 1067, 1256, 1372
BseLI CCNNNNNNNGG 5 cut(s) 17, 296, 507, 796, 1444
BseMI GCAATG 4 cut(s) 84, 168, 1043, 1296
BseMII CTCAG 1 cut(s) 992
BseNI ACTGG 3 cut(s) 1088, 1346, 1438
BseRI GAGGAG 4 cut(s) 211, 450, 1347, 1350
BseXI GCAGC 1 cut(s) 1322
Bsh1236I CGCG 3 cut(s) 107, 180, 251
BshFI GGCC 1 cut(s) 936
BshTI ACCGGT 1 cut(s) 546
BsiHKCI CYCGRG 2 cut(s) 432, 961
BsiSI CCGG 2 cut(s) 547, 563
BslFI GGGAC 1 cut(s) 1415
BslI CCNNNNNNNGG 5 cut(s) 17, 296, 507, 796, 1444
BsmAI GTCTC 9 cut(s) 91, 124, 335, 432, 435, 527, 1031, 1332, 1392
BsmBI CGTCTC 3 cut(s) 335, 432, 435
BsmFI GGGAC 1 cut(s) 1415
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 1 cut(s) 936
Bso31I GGTCTC 1 cut(s) 1031
BsoBI CYCGRG 2 cut(s) 432, 961
Bsp143I GATC 4 cut(s) 595, 745, 813, 1230
Bsp19I CCATGG 1 cut(s) 1376
Bsp68I TCGCGA 1 cut(s) 107
BspACI CCGC 3 cut(s) 166, 297, 1307
BspANI GGCC 1 cut(s) 936
BspCNI CTCAG 1 cut(s) 991
BspFNI CGCG 3 cut(s) 107, 180, 251
BspLI GGNNCC 3 cut(s) 499, 747, 989
BspMI ACCTGC 1 cut(s) 476
BspPI GGATC 3 cut(s) 740, 753, 808
BspTNI GGTCTC 1 cut(s) 1031
BsrDI GCAATG 4 cut(s) 84, 168, 1043, 1296
BsrFI RCCGGY 1 cut(s) 546
BsrI ACTGG 3 cut(s) 1088, 1346, 1438
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 3 cut(s) 1012, 1154, 1376
BssMI GATC 4 cut(s) 595, 745, 813, 1230
BssNI GRCGYC 1 cut(s) 257
BssT1I CCWWGG 2 cut(s) 1154, 1376
Bst2UI CCWGG 1 cut(s) 1014
Bst4CI ACNGT 4 cut(s) 12, 237, 502, 893
BstACI GRCGYC 1 cut(s) 257
BstAPI GCANNNNNTGC 2 cut(s) 680, 1115
BstC8I GCNNGC 2 cut(s) 109, 1257
BstDEI CTNAG 2 cut(s) 415, 978
BstDSI CCRYGG 1 cut(s) 1376
BstEII GGTNACC 1 cut(s) 549
BstF5I GGATG 8 cut(s) 45, 416, 524, 620, 880, 1067, 1256, 1372
BstFNI CGCG 3 cut(s) 107, 180, 251
BstHHI GCGC 1 cut(s) 909
BstKTI GATC 4 cut(s) 598, 748, 816, 1233
BstMAI GTCTC 9 cut(s) 91, 124, 335, 432, 435, 527, 1031, 1332, 1392
BstMBI GATC 4 cut(s) 595, 745, 813, 1230
BstMWI GCNNNNNNNGC 5 cut(s) 680, 884, 1115, 1252, 1307
BstNI CCWGG 1 cut(s) 1014
BstNSI RCATGY 1 cut(s) 1259
BstPI GGTNACC 1 cut(s) 549
BstSCI CCNGG 1 cut(s) 1012
BstSFI CTRYAG 1 cut(s) 225
BstUI CGCG 3 cut(s) 107, 180, 251
BstV1I GCAGC 1 cut(s) 1322
BstV2I GAAGAC 2 cut(s) 271, 838
BstX2I RGATCY 3 cut(s) 745, 813, 1230
BstXI CCANNNNNNTGG 1 cut(s) 639
BstYI RGATCY 3 cut(s) 745, 813, 1230
BsuRI GGCC 1 cut(s) 936
BtgI CCRYGG 1 cut(s) 1376
BtsCI GGATG 8 cut(s) 45, 416, 524, 620, 880, 1067, 1256, 1372
BtsI GCAGTG 1 cut(s) 997
BtsIMutI CAGTG 1 cut(s) 997
BtuMI TCGCGA 1 cut(s) 107
BveI ACCTGC 1 cut(s) 476
Cac8I GCNNGC 2 cut(s) 109, 1257
CfoI GCGC 1 cut(s) 909
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 2 cut(s) 311, 935
CseI GACGC 3 cut(s) 221, 265, 417
Csp6I GTAC 1 cut(s) 1085
CspAI ACCGGT 1 cut(s) 546
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CviAII CATG 2 cut(s) 1256, 1377
CviQI GTAC 1 cut(s) 1085
DdeI CTNAG 2 cut(s) 415, 978
DpnI GATC 4 cut(s) 597, 747, 815, 1232
DpnII GATC 4 cut(s) 595, 745, 813, 1230
DraI TTTAAA 1 cut(s) 762
Eco130I CCWWGG 2 cut(s) 1154, 1376
Eco31I GGTCTC 1 cut(s) 1031
Eco47I GGWCC 1 cut(s) 311
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 2 cut(s) 432, 961
Eco91I GGTNACC 1 cut(s) 549
EcoO65I GGTNACC 1 cut(s) 549
EcoRII CCWGG 1 cut(s) 1012
EcoT14I CCWWGG 2 cut(s) 1154, 1376
ErhI CCWWGG 2 cut(s) 1154, 1376
Esp3I CGTCTC 3 cut(s) 335, 432, 435
FaeI CATG 2 cut(s) 1259, 1380
FalI AAGNNNNNCTT 6 cut(s) 117, 149, 1138, 1170, 1164, 1196
FaqI GGGAC 1 cut(s) 1415
FatI CATG 2 cut(s) 1255, 1376
Fnu4HI GCNGC 2 cut(s) 1308, 1311
FokI GGATG 8 cut(s) 52, 423, 531, 627, 887, 1054, 1263, 1379
Fsp4HI GCNGC 2 cut(s) 1308, 1311
FspBI CTAG 1 cut(s) 515
FspI TGCGCA 1 cut(s) 908
GlaI GCGC 1 cut(s) 908
GluI GCNGC 2 cut(s) 1308, 1311
GsuI CTGGAG 2 cut(s) 317, 1343
HaeIII GGCC 1 cut(s) 936
HapII CCGG 2 cut(s) 547, 563
HgaI GACGC 3 cut(s) 221, 265, 417
HhaI GCGC 1 cut(s) 909
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 2 cut(s) 1259, 1380
Hin6I GCGC 1 cut(s) 907
HinP1I GCGC 1 cut(s) 907
HincII GTYRAC 2 cut(s) 255, 1417
HindII GTYRAC 2 cut(s) 255, 1417
HpaII CCGG 2 cut(s) 547, 563
HphI GGTGA 5 cut(s) 32, 539, 746, 890, 1427
Hpy166II GTNNAC 4 cut(s) 255, 314, 530, 1417
Hpy188I TCNGA 5 cut(s) 74, 584, 614, 717, 1072
Hpy188III TCNNGA 9 cut(s) 62, 106, 334, 432, 461, 603, 963, 1030, 1181
Hpy8I GTNNAC 4 cut(s) 255, 314, 530, 1417
Hpy99I CGWCG 3 cut(s) 16, 215, 829
HpyAV CCTTC 3 cut(s) 217, 738, 833
HpyCH4III ACNGT 4 cut(s) 12, 237, 502, 893
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 9 cut(s) 173, 186, 485, 929, 1002, 1048, 1118, 1301, 1360
HpyF10VI GCNNNNNNNGC 5 cut(s) 680, 884, 1115, 1252, 1307
HpyF3I CTNAG 2 cut(s) 415, 978
HpySE526I ACGT 1 cut(s) 328
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 2 cut(s) 1259, 1380
HspAI GCGC 1 cut(s) 907
Kzo9I GATC 4 cut(s) 595, 745, 813, 1230
LmnI GCTCC 2 cut(s) 74, 688
Lsp1109I GCAGC 1 cut(s) 1322
LweI GCATC 3 cut(s) 401, 865, 1339
MaeI CTAG 1 cut(s) 515
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 2 cut(s) 549, 779
MalI GATC 4 cut(s) 597, 747, 815, 1232
MboI GATC 4 cut(s) 595, 745, 813, 1230
MboII GAAGA 5 cut(s) 182, 271, 362, 838, 1158
MflI RGATCY 3 cut(s) 745, 813, 1230
MluCI AATT 5 cut(s) 474, 535, 738, 793, 853
MluI ACGCGT 1 cut(s) 249
MlyI GAGTC 5 cut(s) 59, 126, 852, 1058, 1437
MmeI TCCRAC 2 cut(s) 686, 1041
MseI TTAA 8 cut(s) 285, 321, 368, 534, 761, 1019, 1241, 1422
MslI CAYNNNNRTG 4 cut(s) 637, 775, 1072, 1134
MspI CCGG 2 cut(s) 547, 563
MspR9I CCNGG 1 cut(s) 1014
Mva1269I GAATGC 1 cut(s) 186
MvaI CCWGG 1 cut(s) 1014
MvnI CGCG 3 cut(s) 107, 180, 251
MwoI GCNNNNNNNGC 5 cut(s) 680, 884, 1115, 1252, 1307
NcoI CCATGG 1 cut(s) 1376
NdeII GATC 4 cut(s) 595, 745, 813, 1230
NlaIII CATG 2 cut(s) 1259, 1380
NlaIV GGNNCC 3 cut(s) 499, 747, 989
NruI TCGCGA 1 cut(s) 107
NsbI TGCGCA 1 cut(s) 908
NspI RCATGY 1 cut(s) 1259
PaeI GCATGC 1 cut(s) 1259
PaeR7I CTCGAG 1 cut(s) 432
PaqCI CACCTGC 1 cut(s) 476
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 1 cut(s) 186
PfeI GAWTC 7 cut(s) 5, 23, 43, 203, 337, 457, 1123
PflMI CCANNNNNTGG 2 cut(s) 796, 1444
PinAI ACCGGT 1 cut(s) 546
PkrI GCNGC 2 cut(s) 1309, 1312
PleI GAGTC 5 cut(s) 59, 125, 851, 1058, 1436
PpsI GAGTC 5 cut(s) 59, 125, 851, 1058, 1436
Psp6I CCWGG 1 cut(s) 1012
PspEI GGTNACC 1 cut(s) 549
PspGI CCWGG 1 cut(s) 1012
PspN4I GGNNCC 3 cut(s) 499, 747, 989
PspPI GGNCC 2 cut(s) 311, 935
PsrI GAACNNNNNNTAC 2 cut(s) 522, 554
PsuI RGATCY 3 cut(s) 745, 813, 1230
RruI TCGCGA 1 cut(s) 107
RsaI GTAC 1 cut(s) 1086
RsaNI GTAC 1 cut(s) 1085
RseI CAYNNNNRTG 4 cut(s) 637, 775, 1072, 1134
SaqAI TTAA 8 cut(s) 285, 321, 368, 534, 761, 1019, 1241, 1422
SatI GCNGC 2 cut(s) 1308, 1311
Sau3AI GATC 4 cut(s) 595, 745, 813, 1230
Sau96I GGNCC 2 cut(s) 311, 935
SchI GAGTC 5 cut(s) 59, 126, 852, 1058, 1437
ScrFI CCNGG 1 cut(s) 1014
SfaNI GCATC 3 cut(s) 401, 865, 1339
SfcI CTRYAG 1 cut(s) 225
Sfr274I CTCGAG 1 cut(s) 432
SinI GGWCC 1 cut(s) 311
SlaI CTCGAG 1 cut(s) 432
SmiMI CAYNNNNRTG 4 cut(s) 637, 775, 1072, 1134
SmlI CTYRAG 4 cut(s) 432, 879, 1028, 1226
SmoI CTYRAG 4 cut(s) 432, 879, 1028, 1226
SphI GCATGC 1 cut(s) 1259
Sse9I AATT 5 cut(s) 474, 535, 738, 793, 853
SsiI CCGC 3 cut(s) 166, 297, 1307
SspI AATATT 1 cut(s) 712
SspMI CTAG 1 cut(s) 515
StyD4I CCNGG 1 cut(s) 1012
StyI CCWWGG 2 cut(s) 1154, 1376
TaaI ACNGT 4 cut(s) 12, 237, 502, 893
TaiI ACGT 1 cut(s) 331
TaqI TCGA 7 cut(s) 14, 63, 210, 397, 433, 569, 824
TasI AATT 5 cut(s) 474, 535, 738, 793, 853
TauI GCSGC 1 cut(s) 1310
TfiI GAWTC 7 cut(s) 5, 23, 43, 203, 337, 457, 1123
Tru1I TTAA 8 cut(s) 285, 321, 368, 534, 761, 1019, 1241, 1422
Tru9I TTAA 8 cut(s) 285, 321, 368, 534, 761, 1019, 1241, 1422
TscAI CASTG 1 cut(s) 1004
TseI GCWGC 1 cut(s) 1310
TspDTI ATGAA 6 cut(s) 15, 45, 95, 615, 656, 852
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 1 cut(s) 1004
Van91I CCANNNNNTGG 2 cut(s) 796, 1444
VpaK11BI GGWCC 1 cut(s) 311
XapI RAATTY 1 cut(s) 793
XceI RCATGY 1 cut(s) 1259
XhoI CTCGAG 1 cut(s) 432
XspI CTAG 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.