AT2G20590

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011237
Physical Location & Seq
Reverse (-)
8867040 .. 8869329
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20590.2

Sequence Viewer

Length: 1296 bp
ATGGAGTCTACGCCTCCTTATCACCGATCCAACACTAAATCAGCTTCGCGTCTCCAAGATTCTTCGAATCCTCCAAATCTCTCTCTTGATTTGGTTCTGTCGTCTCCCAATACTCCAAACCCATCATCACCTGTTCCTCTTCGCGATATCCTCCTCTTACCTCCTTCTCCACTTCGAAAATCGAGGACCCGTTTATCCGATCGACTCGAAATGACTTCTGAAGATGCTATGGCGGTGGTTCGGAAACGGGGCAAAGGGAAAGGAGGGCAAAAGAGTCTTTTAGCATCTCCGAGGAATCCCCGGAGATCGAGGCGAAGATCTGAAGCTGTGGAAGAGAAAGAAGCTAATTTGGTTATCGAAGAAATCGTAAAGCTTCCTCCTAGAAAGCGGAAGACTAATGGTCGACCTAAGAAGGATAAGCAAAGTTCAGCTCCTCCTCTTTGTTCTTCCTCAGATCTTCCAAATACATGTCAGAGCGATTTGAATCTGATTGGAGAGATTATAAGTGATTTGGTAATGTGGAGAGATGTTGCGAAATCGACTCTCTGGTTTGGTTTTGGATGTTTGTCTTTCCTATCTTCTTGCTTTGCCAAAGGAGTCAACTTTAGCGTTTTCTCGGCGGTTTCCAATCTCGGACTTGTGTTACTTTGCGGGTCCTTCTTGTCAAACACTCTTTGTCAAAGGAAAAATGAAGACACAAAGAGAGCGTTCCATGTGAGTGAAGATGATGTCTTGCGCTCAGCTAGACGAGTGCTTCCTGCAACCAACTTTTTTATCTCGAAGACTAGTGAGCTTTTTTCAGGAGAACCATCAATGACACTCAAAGTGACACCATTTCTGCTACTTGGAGCTGAGTATGGTCATCTCATAACACTGTGGAGGCTATCTGCATTTGGTTTCTTCCTCAGTTTCACCATCCCAAAGCTGTATTCATGTTACACGCATCAGATTAGCCAAAAAGTTGAAAGAGTGAAAACGAGAATAGGTGAAGCATGGGGAGTCTGCTCACACAAGAAGATCTTGGCTGGCTCTGCGGTGACAGCGTTTTGGAACTTGACGAGCATAAGAACCCGAATATTTGCAGTTTTTATCATCCTGGTGATATTTCGGTATAGGAGACAAAATCTACAGCTAACTCCCGAGGAAGTTGAGCCTGTTGAGAATGAGCAAGAAGAAGAAACACTCCCGCAGGAAGAAGAAACAGTTCCACAAGAAGAAGAAACAGTTCCACAAGAAGAAGAACAGACACAGCCATCAGAAGAGCGAGCTTTAGTGGTGGTGGTTGCAGAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

431

Amino Acids

48.4

Weight (kDa)

8.93

Isoelectric Point (pI)

66.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 169 - 320 6.7e-32 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011237)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20590 AT2G20590 AT2G20590
fragaria_vesca FvH4_6g26620 FvH4_6g26620 FvH4_6g26620
malus_domestica MD09G1242900.v1.1
prunus_persica Prupe.3G132900_v2.0.a1 Prupe.3G133200_v2.0.a1
pyrus_communis pycom09g16120
rosa_chinensis RchiOBHm_Chr2g0127351
rosa_laevigata RLG00000018965
rosa_multiflora Rmu_ssc0000018.1_g000044
rosa_roxburghii Rroxscaffold_2G00116360
rosa_rugosa Rorug02G0275000
rosa_samantha Rh2AG326100 Rh2BG335600 Rh2CG313800 Rh2DG353300
rosa_wichuraiana Rw2G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 503
AccI GTMKAC 2 cut(s) 8, 403
AccII CGCG 2 cut(s) 49, 144
AciI CCGC 6 cut(s) 233, 388, 620, 651, 1034, 1187
AclWI GGATC 1 cut(s) 21
AcuI CTGAAG 2 cut(s) 240, 342
AflIII ACRYGT 1 cut(s) 467
AgsI TTSAA 2 cut(s) 484, 965
AhdI GACNNNNNGTC 1 cut(s) 399
AhlI ACTAGT 1 cut(s) 785
AjnI CCWGG 1 cut(s) 1095
AjuI GAANNNNNNNTTGG 2 cut(s) 913, 945
Alw26I GTCTC 3 cut(s) 56, 108, 1111
AlwI GGATC 1 cut(s) 21
Ama87I CYCGRG 1 cut(s) 1139
Asp700I GAANNNNTTC 2 cut(s) 1203, 1224
AspLEI GCGC 1 cut(s) 738
AspS9I GGNCC 2 cut(s) 186, 654
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 6 cut(s) 14, 120, 904, 998, 1048, 1111
AsuII TTCGAA 2 cut(s) 65, 175
AvaI CYCGRG 1 cut(s) 1139
AvaII GGWCC 2 cut(s) 186, 654
BbsI GAAGAC 3 cut(s) 398, 699, 788
BccI CCATC 4 cut(s) 130, 817, 923, 1261
BciT130I CCWGG 1 cut(s) 1097
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 3 cut(s) 56, 108, 1111
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 3 cut(s) 381, 744, 786
BfmI CTRYAG 1 cut(s) 1127
BglII AGATCT 3 cut(s) 317, 454, 1017
BlpI GCTNAGC 1 cut(s) 739
Bme1390I CCNGG 2 cut(s) 301, 1097
Bme18I GGWCC 2 cut(s) 186, 654
BmeRI GACNNNNNGTC 1 cut(s) 399
BmeT110I CYCGRG 1 cut(s) 1139
BmgT120I GGNCC 2 cut(s) 186, 654
BmiI GGNNCC 2 cut(s) 188, 655
BmrFI CCNGG 2 cut(s) 301, 1097
BmsI GCATC 3 cut(s) 214, 293, 952
BpiI GAAGAC 3 cut(s) 398, 699, 788
Bpu1102I GCTNAGC 1 cut(s) 739
Bpu14I TTCGAA 2 cut(s) 65, 175
BpuMI CCSGG 1 cut(s) 301
BsaBI GATNNNNATC 1 cut(s) 483
BsaJI CCNNGG 3 cut(s) 290, 299, 1140
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 1 cut(s) 1097
BseDI CCNNGG 3 cut(s) 290, 299, 1140
BseGI GGATG 3 cut(s) 566, 915, 1092
BseJI GATNNNNATC 1 cut(s) 483
BseMII CTCAG 4 cut(s) 465, 753, 843, 919
BseRI GAGGAG 3 cut(s) 143, 423, 426
Bsh1236I CGCG 2 cut(s) 49, 144
Bsh1285I CGRYCG 1 cut(s) 202
BsiEI CGRYCG 1 cut(s) 202
BsiHKCI CYCGRG 1 cut(s) 1139
BsiSI CCGG 1 cut(s) 301
BsmAI GTCTC 3 cut(s) 56, 108, 1111
BsmBI CGTCTC 2 cut(s) 56, 108
BsoBI CYCGRG 1 cut(s) 1139
Bsp119I TTCGAA 2 cut(s) 65, 175
Bsp143I GATC 6 cut(s) 26, 199, 305, 317, 454, 1017
Bsp1720I GCTNAGC 1 cut(s) 739
Bsp68I TCGCGA 1 cut(s) 144
BspACI CCGC 6 cut(s) 233, 388, 620, 651, 1034, 1187
BspCNI CTCAG 4 cut(s) 464, 752, 844, 918
BspFNI CGCG 2 cut(s) 49, 144
BspLI GGNNCC 2 cut(s) 188, 655
BspPI GGATC 1 cut(s) 21
BspQI GCTCTTC 1 cut(s) 1254
BspT104I TTCGAA 2 cut(s) 65, 175
BssECI CCNNGG 3 cut(s) 290, 299, 1140
BssMI GATC 6 cut(s) 26, 199, 305, 317, 454, 1017
Bst2UI CCWGG 1 cut(s) 1097
Bst4CI ACNGT 3 cut(s) 876, 1204, 1225
Bst6I CTCTTC 3 cut(s) 144, 327, 1254
BstBI TTCGAA 2 cut(s) 65, 175
BstC8I GCNNGC 2 cut(s) 1027, 1266
BstDEI CTNAG 5 cut(s) 408, 451, 739, 852, 905
BstF5I GGATG 3 cut(s) 566, 915, 1092
BstFNI CGCG 2 cut(s) 49, 144
BstHHI GCGC 1 cut(s) 738
BstKTI GATC 6 cut(s) 29, 202, 308, 320, 457, 1020
BstMAI GTCTC 3 cut(s) 56, 108, 1111
BstMBI GATC 6 cut(s) 26, 199, 305, 317, 454, 1017
BstMCI CGRYCG 1 cut(s) 202
BstMWI GCNNNNNNNGC 2 cut(s) 1031, 1040
BstNI CCWGG 1 cut(s) 1097
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 299, 1095
BstSFI CTRYAG 1 cut(s) 1127
BstUI CGCG 2 cut(s) 49, 144
BstV2I GAAGAC 3 cut(s) 398, 699, 788
BstX2I RGATCY 3 cut(s) 317, 454, 1017
BstYI RGATCY 3 cut(s) 317, 454, 1017
BtsCI GGATG 3 cut(s) 566, 915, 1092
BtsIMutI CAGTG 1 cut(s) 872
BtuMI TCGCGA 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 1027, 1266
CfoI GCGC 1 cut(s) 738
Cfr13I GGNCC 2 cut(s) 186, 654
CseI GACGC 1 cut(s) 38
CviAII CATG 4 cut(s) 468, 713, 933, 993
DdeI CTNAG 5 cut(s) 408, 451, 739, 852, 905
DpnI GATC 6 cut(s) 28, 201, 307, 319, 456, 1019
DpnII GATC 6 cut(s) 26, 199, 305, 317, 454, 1017
DriI GACNNNNNGTC 1 cut(s) 399
Eam1104I CTCTTC 3 cut(s) 144, 327, 1254
Eam1105I GACNNNNNGTC 1 cut(s) 399
EarI CTCTTC 3 cut(s) 144, 327, 1254
Eco32I GATATC 1 cut(s) 148
Eco47I GGWCC 2 cut(s) 186, 654
Eco57I CTGAAG 2 cut(s) 240, 342
Eco88I CYCGRG 1 cut(s) 1139
EcoO109I RGGNCCY 2 cut(s) 186, 654
EcoRII CCWGG 1 cut(s) 1095
EcoRV GATATC 1 cut(s) 148
Esp3I CGTCTC 2 cut(s) 56, 108
FaeI CATG 4 cut(s) 471, 716, 936, 996
FalI AAGNNNNNCTT 2 cut(s) 1004, 1036
FatI CATG 4 cut(s) 467, 712, 932, 992
FauI CCCGC 2 cut(s) 644, 1194
FblI GTMKAC 2 cut(s) 8, 403
FokI GGATG 3 cut(s) 573, 902, 1079
FspBI CTAG 3 cut(s) 381, 744, 786
GlaI GCGC 1 cut(s) 737
HapII CCGG 1 cut(s) 301
HgaI GACGC 1 cut(s) 38
HhaI GCGC 1 cut(s) 738
Hin1II CATG 4 cut(s) 471, 716, 936, 996
Hin6I GCGC 1 cut(s) 736
HinP1I GCGC 1 cut(s) 736
HincII GTYRAC 2 cut(s) 404, 601
HindII GTYRAC 2 cut(s) 404, 601
HindIII AAGCTT 1 cut(s) 371
HpaII CCGG 1 cut(s) 301
HphI GGTGA 6 cut(s) 14, 120, 904, 998, 1048, 1111
Hpy166II GTNNAC 3 cut(s) 9, 404, 601
Hpy188III TCNNGA 5 cut(s) 86, 143, 778, 801, 1139
Hpy8I GTNNAC 3 cut(s) 9, 404, 601
HpyAV CCTTC 3 cut(s) 174, 406, 667
HpyCH4III ACNGT 3 cut(s) 876, 1204, 1225
HpyCH4V TGCA 4 cut(s) 761, 890, 1082, 1286
HpyF10VI GCNNNNNNNGC 2 cut(s) 1031, 1040
HpyF3I CTNAG 5 cut(s) 408, 451, 739, 852, 905
Hsp92II CATG 4 cut(s) 471, 716, 936, 996
HspAI GCGC 1 cut(s) 736
Kzo9I GATC 6 cut(s) 26, 199, 305, 317, 454, 1017
LguI GCTCTTC 1 cut(s) 1254
LmnI GCTCC 2 cut(s) 436, 848
LweI GCATC 3 cut(s) 214, 293, 952
MaeI CTAG 3 cut(s) 381, 744, 786
MaeIII GTNAC 4 cut(s) 642, 826, 935, 1036
MalI GATC 6 cut(s) 28, 201, 307, 319, 456, 1019
MboI GATC 6 cut(s) 26, 199, 305, 317, 454, 1017
MflI RGATCY 3 cut(s) 317, 454, 1017
MluCI AATT 1 cut(s) 346
MlyI GAGTC 6 cut(s) 14, 198, 283, 535, 606, 1008
MmeI TCCRAC 1 cut(s) 54
MroXI GAANNNNTTC 2 cut(s) 1203, 1224
MseI TTAA 1 cut(s) 1294
MslI CAYNNNNRTG 2 cut(s) 717, 1097
MspI CCGG 1 cut(s) 301
MspR9I CCNGG 2 cut(s) 301, 1097
MvaI CCWGG 1 cut(s) 1097
MvnI CGCG 2 cut(s) 49, 144
MwoI GCNNNNNNNGC 2 cut(s) 1031, 1040
NciI CCSGG 1 cut(s) 301
NdeII GATC 6 cut(s) 26, 199, 305, 317, 454, 1017
NlaIII CATG 4 cut(s) 471, 716, 936, 996
NlaIV GGNNCC 2 cut(s) 188, 655
NmeAIII GCCGAG 1 cut(s) 596
NmuCI GTSAC 2 cut(s) 826, 1036
NruI TCGCGA 1 cut(s) 144
NspI RCATGY 1 cut(s) 471
NspV TTCGAA 2 cut(s) 65, 175
PciI ACATGT 1 cut(s) 467
PciSI GCTCTTC 1 cut(s) 1254
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PdmI GAANNNNTTC 2 cut(s) 1203, 1224
PfeI GAWTC 4 cut(s) 59, 67, 295, 484
Ple19I CGATCG 1 cut(s) 202
PleI GAGTC 6 cut(s) 13, 198, 282, 535, 605, 1007
PpsI GAGTC 6 cut(s) 13, 198, 282, 535, 605, 1007
PpuMI RGGWCCY 2 cut(s) 186, 654
PscI ACATGT 1 cut(s) 467
PsiI TTATAA 1 cut(s) 503
Psp5II RGGWCCY 2 cut(s) 186, 654
Psp6I CCWGG 1 cut(s) 1095
PspGI CCWGG 1 cut(s) 1095
PspN4I GGNNCC 2 cut(s) 188, 655
PspPI GGNCC 2 cut(s) 186, 654
PspPPI RGGWCCY 2 cut(s) 186, 654
PsuI RGATCY 3 cut(s) 317, 454, 1017
PvuI CGATCG 1 cut(s) 202
RruI TCGCGA 1 cut(s) 144
RseI CAYNNNNRTG 2 cut(s) 717, 1097
SalI GTCGAC 1 cut(s) 402
SapI GCTCTTC 1 cut(s) 1254
SaqAI TTAA 1 cut(s) 1294
Sau3AI GATC 6 cut(s) 26, 199, 305, 317, 454, 1017
Sau96I GGNCC 2 cut(s) 186, 654
SchI GAGTC 6 cut(s) 14, 198, 283, 535, 606, 1008
ScrFI CCNGG 2 cut(s) 301, 1097
SfaNI GCATC 3 cut(s) 214, 293, 952
SfcI CTRYAG 1 cut(s) 1127
SfuI TTCGAA 2 cut(s) 65, 175
SinI GGWCC 2 cut(s) 186, 654
SmiMI CAYNNNNRTG 2 cut(s) 717, 1097
SpeI ACTAGT 1 cut(s) 785
Sse9I AATT 1 cut(s) 346
SsiI CCGC 6 cut(s) 233, 388, 620, 651, 1034, 1187
SspI AATATT 1 cut(s) 1077
SspMI CTAG 3 cut(s) 381, 744, 786
StyD4I CCNGG 2 cut(s) 299, 1095
TaaI ACNGT 3 cut(s) 876, 1204, 1225
TasI AATT 1 cut(s) 346
TfiI GAWTC 4 cut(s) 59, 67, 295, 484
Tru1I TTAA 1 cut(s) 1294
Tru9I TTAA 1 cut(s) 1294
TscAI CASTG 1 cut(s) 879
TseFI GTSAC 2 cut(s) 826, 1036
Tsp45I GTSAC 2 cut(s) 826, 1036
TspDTI ATGAA 2 cut(s) 705, 921
TspRI CASTG 1 cut(s) 879
VpaK11BI GGWCC 2 cut(s) 186, 654
XceI RCATGY 1 cut(s) 471
XmiI GTMKAC 2 cut(s) 8, 403
XmnI GAANNNNTTC 2 cut(s) 1203, 1224
XspI CTAG 3 cut(s) 381, 744, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.