Rroxscaffold_2G00116360

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011237
Physical Location & Seq
Reverse (-)
43450523 .. 43458737
8215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116360.1

Sequence Viewer

Length: 1287 bp
ATGGATTCGACAGCTTCACTGCCTCCCTATCGTCACCATTCTCGTTCGGAGCCACCGTCCCGAACCAAAACGGCGTCACGGCTCGCCCAATCGGAGAAGGTCCCACATCTCTCTCTGGGTCTCATTCAATCACCTTCACCTCCGTCTCGAAAATCTTCAGCTTCTCTCCCTCTACACGAGCTGCTGCTACTCTCGCCTTCGCCTAATCCAAGAAGGTCCAAGACCCGTCTCGCCGATCGGTTTGAAATGCCCGATGAGCCGGTGGCGGAACCGGCAGGCCCACGACGGAGGTGCAAGAGCAGCAGAGGCTCCCAGATGGGCTCTCTGGGCTGTGCCTCGCCCAGGAACACCAGAAGGTCCAGGAGGAGATCGGAGGTTGAGATTCGAGAAGATAAGGATTTGGGTTTGGGGGAAGAGATTGTCAAGGTCAGGAAAAGAAGGTCCAAGAAGGACAAGCTGAGCTTGGTGCCTTGTGTGCTGCCGTCCCCAACTTCCTCTACGATGGATGATGACGATGGAGGTCGTCTGGAGCGAATTGGGCAGCTATTGAATGATCTGATCATGTGGAGAGATGTAGCAAGATCGACCCTTTGGTTTGGTTTGGGCTCTCTGTTCTTCTTGTCTTCTTGCTTTGCCAAAGGAGTGAACTTTAGCGTTTTCTCGGCGATTTCCCAACTAGGACTTCTGTTCTTGGGTGCTTCATTTGTCTCGAATTCAATCTCCCAAAGAAATGGTATCGAAAAGAAGCGGGATTACTTGCTTAAGGAAAATGATATCCTTGGTGCGGCCAAAATGATACTCCCTGCAGCAAATCTTGCAATATCAAGTACAAGGAAGCTTTTCTCAGGAGAGCCATCTATGACTCTCAAGGTAGCTCCATTCTTTCTTCTAGGAGCAGAGTATGGACATCATATAACATTGTGGAGGCTCTCTGTGCTTGCATTCTTTATCAGCTTCACAATCCCAAGGCTGTACTCATGCTACTCCATTCAAATGAACCAAAGAGCTGAGTGCTTGAAATGGTGGGTGTTGGAAGCATGGGGAGCATGCTCTCACAAAAAGATTGTAGCAGCATCAGCAGCCACAGCATTCTGGAATCTGTCTACGGTGAAAACCCGCATATTCACCGCATTCATATCCCTTGTAATTCTTCGTTACTGTCGACAACACATGGTGCAAGTTACGGAAGCAGAACAGGAGGAGCAGCAGCAGGCCATATTAGTGGCAGAAGCAGAAGGAAAACAAGAGCAGGCATTAGTTGTGGCCGAAGTGGGCAAGCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

428

Amino Acids

47.7

Weight (kDa)

9.81

Isoelectric Point (pI)

67.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 184 - 337 7.6e-27 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011237)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20590 AT2G20590 AT2G20590
fragaria_vesca FvH4_6g26620 FvH4_6g26620 FvH4_6g26620
malus_domestica MD09G1242900.v1.1
prunus_persica Prupe.3G132900_v2.0.a1 Prupe.3G133200_v2.0.a1
pyrus_communis pycom09g16120
rosa_chinensis RchiOBHm_Chr2g0127351
rosa_laevigata RLG00000018965
rosa_multiflora Rmu_ssc0000018.1_g000044
rosa_roxburghii Rroxscaffold_2G00116360
rosa_rugosa Rorug02G0275000
rosa_samantha Rh2AG326100 Rh2BG335600 Rh2CG313800 Rh2DG353300
rosa_wichuraiana Rw2G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 466
AccI GTMKAC 2 cut(s) 1103, 1162
AciI CCGC 5 cut(s) 266, 748, 785, 1117, 1128
AcoI YGGCCR 2 cut(s) 786, 1263
AcsI RAATTY 1 cut(s) 712
AcuI CTGAAG 1 cut(s) 141
AcyI GRCGYC 1 cut(s) 74
AdeI CACNNNGTG 1 cut(s) 1174
AfaI GTAC 2 cut(s) 829, 974
AfiI CCNNNNNNNGG 2 cut(s) 342, 784
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 6 cut(s) 128, 245, 550, 717, 992, 1018
AjnI CCWGG 2 cut(s) 341, 359
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
Alw26I GTCTC 4 cut(s) 125, 150, 233, 712
AoxI GGCC 4 cut(s) 277, 786, 1212, 1263
ApoI RAATTY 1 cut(s) 712
Asp700I GAANNNNTTC 2 cut(s) 154, 839
AspS9I GGNCC 5 cut(s) 100, 216, 278, 357, 441
AsuHPI GGTGA 5 cut(s) 26, 123, 129, 1117, 1120
AvaII GGWCC 4 cut(s) 100, 216, 357, 441
BanI GGYRCC 1 cut(s) 466
BanII GRGCYC 2 cut(s) 323, 608
BarI GAAGNNNNNNTAC 2 cut(s) 737, 769
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 615
BccI CCATC 4 cut(s) 310, 496, 509, 862
BceAI ACGGC 3 cut(s) 87, 95, 466
BcgI CGANNNNNNTGC 2 cut(s) 273, 307
BciT130I CCWGG 2 cut(s) 343, 361
BclI TGATCA 1 cut(s) 558
BcoDI GTCTC 4 cut(s) 125, 150, 233, 712
BfaI CTAG 2 cut(s) 677, 890
BfmI CTRYAG 1 cut(s) 804
BfrI CTTAAG 1 cut(s) 761
BlpI GCTNAGC 1 cut(s) 458
Bme1390I CCNGG 2 cut(s) 343, 361
Bme18I GGWCC 4 cut(s) 100, 216, 357, 441
BmgT120I GGNCC 5 cut(s) 100, 216, 278, 357, 441
BmiI GGNNCC 5 cut(s) 51, 102, 270, 310, 468
BmrFI CCNGG 2 cut(s) 343, 361
BmsI GCATC 1 cut(s) 1082
BpiI GAAGAC 1 cut(s) 615
BpmI CTGGAG 1 cut(s) 548
Bpu1102I GCTNAGC 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 851
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 3 cut(s) 341, 778, 965
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 784
Bse118I RCCGGY 2 cut(s) 259, 271
BseBI CCWGG 2 cut(s) 343, 361
BseDI CCNNGG 3 cut(s) 341, 778, 965
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 2 cut(s) 342, 784
BseMII CTCAG 3 cut(s) 449, 858, 999
BseRI GAGGAG 2 cut(s) 379, 1214
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 4 cut(s) 279, 788, 1214, 1265
BshNI GGYRCC 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 238
BsiSI CCGG 2 cut(s) 260, 272
BslFI GGGAC 3 cut(s) 43, 86, 469
BslI CCNNNNNNNGG 2 cut(s) 342, 784
BsmAI GTCTC 4 cut(s) 125, 150, 233, 712
BsmBI CGTCTC 2 cut(s) 150, 233
BsmFI GGGAC 3 cut(s) 43, 86, 469
BsmI GAATGC 3 cut(s) 941, 1088, 1130
BsnI GGCC 4 cut(s) 279, 788, 1214, 1265
Bso31I GGTCTC 1 cut(s) 125
Bsp1286I GDGCHC 2 cut(s) 323, 608
Bsp143I GATC 5 cut(s) 235, 368, 553, 558, 581
Bsp1720I GCTNAGC 1 cut(s) 458
BspACI CCGC 5 cut(s) 266, 748, 785, 1117, 1128
BspANI GGCC 4 cut(s) 279, 788, 1214, 1265
BspCNI CTCAG 3 cut(s) 450, 857, 1000
BspLI GGNNCC 5 cut(s) 51, 102, 270, 310, 468
BspMAI CTGCAG 1 cut(s) 808
BspT107I GGYRCC 1 cut(s) 466
BspTI CTTAAG 1 cut(s) 761
BspTNI GGTCTC 1 cut(s) 125
BsrFI RCCGGY 2 cut(s) 259, 271
BssAI RCCGGY 2 cut(s) 259, 271
BssECI CCNNGG 3 cut(s) 341, 778, 965
BssMI GATC 5 cut(s) 235, 368, 553, 558, 581
BssNI GRCGYC 1 cut(s) 74
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 2 cut(s) 778, 965
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 2 cut(s) 343, 361
Bst4CI ACNGT 3 cut(s) 57, 1108, 1160
Bst6I CTCTTC 1 cut(s) 408
BstACI GRCGYC 1 cut(s) 74
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 1 cut(s) 815
BstC8I GCNNGC 7 cut(s) 84, 277, 939, 1048, 1212, 1251, 1277
BstDEI CTNAG 3 cut(s) 458, 844, 1008
BstENI CCTNNNNNAGG 1 cut(s) 340
BstF5I GGATG 1 cut(s) 511
BstKTI GATC 5 cut(s) 238, 371, 556, 561, 584
BstMAI GTCTC 4 cut(s) 125, 150, 233, 712
BstMBI GATC 5 cut(s) 235, 368, 553, 558, 581
BstMCI CGRYCG 1 cut(s) 238
BstNI CCWGG 2 cut(s) 343, 361
BstNSI RCATGY 1 cut(s) 1050
BstSCI CCNGG 2 cut(s) 341, 359
BstSFI CTRYAG 1 cut(s) 804
BstV2I GAAGAC 1 cut(s) 615
BstXI CCANNNNNNTGG 2 cut(s) 731, 1222
BsuRI GGCC 4 cut(s) 279, 788, 1214, 1265
BtsCI GGATG 1 cut(s) 511
BtsI GCAGTG 1 cut(s) 17
BtsIMutI CAGTG 1 cut(s) 17
Cac8I GCNNGC 7 cut(s) 84, 277, 939, 1048, 1212, 1251, 1277
Cfr10I RCCGGY 2 cut(s) 259, 271
Cfr13I GGNCC 5 cut(s) 100, 216, 278, 357, 441
CseI GACGC 1 cut(s) 63
Csp6I GTAC 2 cut(s) 828, 973
CviAII CATG 5 cut(s) 562, 978, 1038, 1047, 1171
CviQI GTAC 2 cut(s) 828, 973
DdeI CTNAG 3 cut(s) 458, 844, 1008
DpnI GATC 5 cut(s) 237, 370, 555, 560, 583
DpnII GATC 5 cut(s) 235, 368, 553, 558, 581
DraIII CACNNNGTG 1 cut(s) 1174
EaeI YGGCCR 2 cut(s) 786, 1263
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 778, 965
Eco24I GRGCYC 2 cut(s) 323, 608
Eco31I GGTCTC 1 cut(s) 125
Eco32I GATATC 1 cut(s) 775
Eco47I GGWCC 4 cut(s) 100, 216, 357, 441
Eco57I CTGAAG 1 cut(s) 141
EcoNI CCTNNNNNAGG 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 712
EcoRII CCWGG 2 cut(s) 341, 359
EcoRV GATATC 1 cut(s) 775
EcoT14I CCWWGG 2 cut(s) 778, 965
EcoT38I GRGCYC 2 cut(s) 323, 608
ErhI CCWWGG 2 cut(s) 778, 965
Esp3I CGTCTC 2 cut(s) 150, 233
FaeI CATG 5 cut(s) 565, 981, 1041, 1050, 1174
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 3 cut(s) 43, 86, 469
FatI CATG 5 cut(s) 561, 977, 1037, 1046, 1170
FauI CCCGC 2 cut(s) 741, 1124
FbaI TGATCA 1 cut(s) 558
FblI GTMKAC 2 cut(s) 1103, 1162
FokI GGATG 1 cut(s) 518
FriOI GRGCYC 2 cut(s) 323, 608
FspBI CTAG 2 cut(s) 677, 890
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 4 cut(s) 279, 788, 1214, 1265
HapII CCGG 2 cut(s) 260, 272
HgaI GACGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 5 cut(s) 565, 981, 1041, 1050, 1174
HincII GTYRAC 1 cut(s) 1163
HindII GTYRAC 1 cut(s) 1163
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 4 cut(s) 5, 382, 862, 1096
HpaII CCGG 2 cut(s) 260, 272
HphI GGTGA 5 cut(s) 26, 123, 129, 1117, 1120
Hpy166II GTNNAC 3 cut(s) 646, 1104, 1163
Hpy188I TCNGA 4 cut(s) 49, 94, 373, 558
Hpy188III TCNNGA 8 cut(s) 60, 147, 386, 430, 527, 709, 846, 1093
Hpy8I GTNNAC 3 cut(s) 646, 1104, 1163
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 8 cut(s) 91, 144, 207, 207, 348, 432, 442, 1229
HpyCH4III ACNGT 3 cut(s) 57, 1108, 1160
HpyCH4V TGCA 5 cut(s) 294, 806, 818, 941, 1177
HpyF3I CTNAG 3 cut(s) 458, 844, 1008
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 5 cut(s) 565, 981, 1041, 1050, 1174
Ksp22I TGATCA 1 cut(s) 558
Kzo9I GATC 5 cut(s) 235, 368, 553, 558, 581
LmnI GCTCC 7 cut(s) 49, 314, 529, 880, 893, 1043, 1201
LweI GCATC 1 cut(s) 1082
MaeI CTAG 2 cut(s) 677, 890
MaeIII GTNAC 4 cut(s) 32, 75, 1154, 1180
MalI GATC 5 cut(s) 237, 370, 555, 560, 583
MboI GATC 5 cut(s) 235, 368, 553, 558, 581
MboII GAAGA 7 cut(s) 147, 401, 425, 607, 615, 878, 1142
MhlI GDGCHC 2 cut(s) 323, 608
MluCI AATT 3 cut(s) 534, 712, 1146
MlyI GAGTC 1 cut(s) 856
MmeI TCCRAC 1 cut(s) 1011
MroXI GAANNNNTTC 2 cut(s) 154, 839
MseI TTAA 1 cut(s) 762
MslI CAYNNNNRTG 2 cut(s) 992, 1220
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 2 cut(s) 260, 272
MspR9I CCNGG 2 cut(s) 343, 361
Mva1269I GAATGC 3 cut(s) 941, 1088, 1130
MvaI CCWGG 2 cut(s) 343, 361
NdeII GATC 5 cut(s) 235, 368, 553, 558, 581
NlaIII CATG 5 cut(s) 565, 981, 1041, 1050, 1174
NlaIV GGNNCC 5 cut(s) 51, 102, 270, 310, 468
NmeAIII GCCGAG 1 cut(s) 641
NmuCI GTSAC 2 cut(s) 32, 75
NspI RCATGY 1 cut(s) 1050
PaeI GCATGC 1 cut(s) 1050
PcsI WCGNNNNNNNCGW 3 cut(s) 53, 529, 1159
PctI GAATGC 3 cut(s) 941, 1088, 1130
PdmI GAANNNNTTC 2 cut(s) 154, 839
PfeI GAWTC 3 cut(s) 5, 382, 1096
PfoI TCCNGGA 1 cut(s) 359
Ple19I CGATCG 1 cut(s) 238
PleI GAGTC 1 cut(s) 856
PpsI GAGTC 1 cut(s) 856
PpuMI RGGWCCY 1 cut(s) 100
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 341, 359
PspGI CCWGG 2 cut(s) 341, 359
PspN4I GGNNCC 5 cut(s) 51, 102, 270, 310, 468
PspPI GGNCC 5 cut(s) 100, 216, 278, 357, 441
PspPPI RGGWCCY 1 cut(s) 100
PstI CTGCAG 1 cut(s) 808
PvuI CGATCG 1 cut(s) 238
RsaI GTAC 2 cut(s) 829, 974
RsaNI GTAC 2 cut(s) 828, 973
RseI CAYNNNNRTG 2 cut(s) 992, 1220
SalI GTCGAC 1 cut(s) 1161
SaqAI TTAA 1 cut(s) 762
Sau3AI GATC 5 cut(s) 235, 368, 553, 558, 581
Sau96I GGNCC 5 cut(s) 100, 216, 278, 357, 441
SchI GAGTC 1 cut(s) 856
ScrFI CCNGG 2 cut(s) 343, 361
SduI GDGCHC 2 cut(s) 323, 608
SfaNI GCATC 1 cut(s) 1082
SfcI CTRYAG 1 cut(s) 804
SinI GGWCC 4 cut(s) 100, 216, 357, 441
SmiMI CAYNNNNRTG 2 cut(s) 992, 1220
SmlI CTYRAG 2 cut(s) 761, 866
SmoI CTYRAG 2 cut(s) 761, 866
SphI GCATGC 1 cut(s) 1050
Sse9I AATT 3 cut(s) 534, 712, 1146
SsiI CCGC 5 cut(s) 266, 748, 785, 1117, 1128
SspMI CTAG 2 cut(s) 677, 890
StyD4I CCNGG 2 cut(s) 341, 359
StyI CCWWGG 2 cut(s) 778, 965
TaaI ACNGT 3 cut(s) 57, 1108, 1160
TaqI TCGA 7 cut(s) 8, 148, 385, 584, 710, 738, 1162
TasI AATT 3 cut(s) 534, 712, 1146
TatI WGTACW 2 cut(s) 827, 972
TauI GCSGC 1 cut(s) 788
TfiI GAWTC 3 cut(s) 5, 382, 1096
Tru1I TTAA 1 cut(s) 762
Tru9I TTAA 1 cut(s) 762
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 2 cut(s) 32, 75
Tsp45I GTSAC 2 cut(s) 32, 75
TspDTI ATGAA 3 cut(s) 690, 1010, 1123
TspGWI ACGGA 3 cut(s) 132, 301, 1199
TspRI CASTG 1 cut(s) 24
Vha464I CTTAAG 1 cut(s) 761
VpaK11BI GGWCC 4 cut(s) 100, 216, 357, 441
XagI CCTNNNNNAGG 1 cut(s) 340
XapI RAATTY 1 cut(s) 712
XceI RCATGY 1 cut(s) 1050
XmiI GTMKAC 2 cut(s) 1103, 1162
XmnI GAANNNNTTC 2 cut(s) 154, 839
XspI CTAG 2 cut(s) 677, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.