Rh2DG353300

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011237
Physical Location & Seq
Forward (+)
49504247 .. 49505794
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG353300.1

Sequence Viewer

Length: 642 bp
ATGGTTGTTTGCAGCGTTTTCTCGGCGATTTCCCAACTAGGACTTCTGTTCTTGGGTGCTTCATTTGTCTCGAATTCAATCTCCCAGAGAAATGGTGTCGAAAAGAAGCGGGATTACTTGCTTAAGGAAAATGATATCCTTGGTGTGGCCAAAATGATACTCCCTGCAGCAAATCTTGCAATATCAAGTACAAGGAAGCTTTTCTCAGGAGAGCCATCTACGACTCTCAAGGTAGCTCCATTCTTTCTTCTAGGAGCAGAGTATGGACATCATATAACAGTGTGGAGGCTCTCTGTCCTTGCATTCTTTATCAGCTTCACAATCCCAAGGCTGTACTCATGCTACTCCATTCAAATGAACCAAAGAGCTGAGTGCTTGAAATGGTGGGTGTTGGAAGCATGGGGAGCATGCTCTCACAAAAAGATTGTAGCAGCATCAGCAGCCACAGCATTCTGGAATCTGTCTACGGTGAAAACCCGCATATTCACCGCATTCATATCCCTTGTAATTCTTCGTTACTGCCGACAACACATGGTGCAAGTTAGGGAAGCCGATGCAGAAGCAGAACAGGAGGAGCAGCAGCGGGCCATAGTAGTGGCAGAAGCAGAAGGAAAACAGGAGCAGGCATTAGTGCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

213

Amino Acids

23.81

Weight (kDa)

9.01

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 4 - 124 5.1e-19 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011237)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20590 AT2G20590 AT2G20590
fragaria_vesca FvH4_6g26620 FvH4_6g26620 FvH4_6g26620
malus_domestica MD09G1242900.v1.1
prunus_persica Prupe.3G132900_v2.0.a1 Prupe.3G133200_v2.0.a1
pyrus_communis pycom09g16120
rosa_chinensis RchiOBHm_Chr2g0127351
rosa_laevigata RLG00000018965
rosa_multiflora Rmu_ssc0000018.1_g000044
rosa_roxburghii Rroxscaffold_2G00116360
rosa_rugosa Rorug02G0275000
rosa_samantha Rh2AG326100 Rh2BG335600 Rh2CG313800 Rh2DG353300
rosa_wichuraiana Rw2G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 464
AciI CCGC 4 cut(s) 109, 478, 489, 583
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 1 cut(s) 73
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 2 cut(s) 190, 335
AfiI CCNNNNNNNGG 1 cut(s) 145
AflII CTTAAG 1 cut(s) 122
AgsI TTSAA 3 cut(s) 78, 353, 379
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 4 cut(s) 199, 236, 315, 368
AluI AGCT 4 cut(s) 199, 236, 315, 368
Alw26I GTCTC 1 cut(s) 73
AoxI GGCC 2 cut(s) 147, 585
ApeKI GCWGC 6 cut(s) 12, 167, 431, 440, 577, 580
ApoI RAATTY 1 cut(s) 73
Asp700I GAANNNNTTC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 585
AsuHPI GGTGA 2 cut(s) 478, 481
BalI TGGCCA 1 cut(s) 149
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BbvI GCAGC 6 cut(s) 24, 179, 443, 452, 589, 592
BccI CCATC 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 73
BfaI CTAG 2 cut(s) 38, 251
BfmI CTRYAG 1 cut(s) 165
BfrI CTTAAG 1 cut(s) 122
BisI GCNGC 6 cut(s) 13, 168, 432, 441, 578, 581
BlsI GCNGC 6 cut(s) 14, 169, 433, 442, 579, 582
BmgT120I GGNCC 1 cut(s) 585
BmsI GCATC 2 cut(s) 443, 544
BpuEI CTTGAG 1 cut(s) 212
BsaJI CCNNGG 2 cut(s) 139, 326
Bsc4I CCNNNNNNNGG 1 cut(s) 145
BseDI CCNNGG 2 cut(s) 139, 326
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 2 cut(s) 219, 360
BseRI GAGGAG 1 cut(s) 587
BseXI GCAGC 6 cut(s) 24, 179, 443, 452, 589, 592
BshFI GGCC 2 cut(s) 149, 587
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 73
BsmI GAATGC 3 cut(s) 302, 449, 491
BsnI GGCC 2 cut(s) 149, 587
BspACI CCGC 4 cut(s) 109, 478, 489, 583
BspANI GGCC 2 cut(s) 149, 587
BspCNI CTCAG 2 cut(s) 218, 361
BspMAI CTGCAG 1 cut(s) 169
BspTI CTTAAG 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 139, 326
BssT1I CCWWGG 2 cut(s) 139, 326
Bst4CI ACNGT 2 cut(s) 280, 469
BstAFI CTTAAG 1 cut(s) 122
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 3 cut(s) 409, 585, 624
BstDEI CTNAG 2 cut(s) 205, 369
BstMAI GTCTC 1 cut(s) 73
BstMWI GCNNNNNNNGC 5 cut(s) 176, 404, 437, 440, 446
BstNSI RCATGY 1 cut(s) 411
BstSFI CTRYAG 1 cut(s) 165
BstV1I GCAGC 6 cut(s) 24, 179, 443, 452, 589, 592
BstXI CCANNNNNNTGG 2 cut(s) 92, 595
BsuRI GGCC 2 cut(s) 149, 587
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 3 cut(s) 409, 585, 624
Cfr13I GGNCC 1 cut(s) 585
Csp6I GTAC 2 cut(s) 189, 334
CviAII CATG 4 cut(s) 339, 399, 408, 532
CviQI GTAC 2 cut(s) 189, 334
DdeI CTNAG 2 cut(s) 205, 369
DraIII CACNNNGTG 1 cut(s) 535
EaeI YGGCCR 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 139, 326
Eco32I GATATC 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 73
EcoRV GATATC 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 139, 326
ErhI CCWWGG 2 cut(s) 139, 326
FaeI CATG 4 cut(s) 342, 402, 411, 535
FatI CATG 4 cut(s) 338, 398, 407, 531
FauI CCCGC 3 cut(s) 102, 485, 576
FblI GTMKAC 1 cut(s) 464
Fnu4HI GCNGC 6 cut(s) 13, 168, 432, 441, 578, 581
Fsp4HI GCNGC 6 cut(s) 13, 168, 432, 441, 578, 581
FspBI CTAG 2 cut(s) 38, 251
GluI GCNGC 6 cut(s) 13, 168, 432, 441, 578, 581
HaeIII GGCC 2 cut(s) 149, 587
Hin1II CATG 4 cut(s) 342, 402, 411, 535
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 2 cut(s) 223, 457
HphI GGTGA 2 cut(s) 478, 481
Hpy166II GTNNAC 1 cut(s) 465
Hpy188III TCNNGA 3 cut(s) 70, 207, 454
Hpy8I GTNNAC 1 cut(s) 465
HpyAV CCTTC 1 cut(s) 602
HpyCH4III ACNGT 2 cut(s) 280, 469
HpyCH4V TGCA 7 cut(s) 12, 167, 179, 302, 538, 557, 634
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 404, 437, 440, 446
HpyF3I CTNAG 2 cut(s) 205, 369
Hsp92II CATG 4 cut(s) 342, 402, 411, 535
LmnI GCTCC 5 cut(s) 241, 254, 404, 574, 619
LpnPI CCDG 7 cut(s) 98, 177, 192, 439, 554, 602, 608
Lsp1109I GCAGC 6 cut(s) 24, 179, 443, 452, 589, 592
LweI GCATC 2 cut(s) 443, 544
MaeI CTAG 2 cut(s) 38, 251
MaeIII GTNAC 1 cut(s) 515
MboII GAAGA 2 cut(s) 239, 503
MlsI TGGCCA 1 cut(s) 149
MluCI AATT 2 cut(s) 73, 507
MluNI TGGCCA 1 cut(s) 149
MlyI GAGTC 1 cut(s) 217
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 2 cut(s) 279, 565
Mox20I TGGCCA 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 200
MscI TGGCCA 1 cut(s) 149
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 2 cut(s) 353, 593
Msp20I TGGCCA 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 583
MspCI CTTAAG 1 cut(s) 122
Mva1269I GAATGC 3 cut(s) 302, 449, 491
MwoI GCNNNNNNNGC 5 cut(s) 176, 404, 437, 440, 446
NlaIII CATG 4 cut(s) 342, 402, 411, 535
NspI RCATGY 1 cut(s) 411
PaeI GCATGC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 520
PctI GAATGC 3 cut(s) 302, 449, 491
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 1 cut(s) 457
PkrI GCNGC 6 cut(s) 14, 169, 433, 442, 579, 582
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 585
PstI CTGCAG 1 cut(s) 169
RsaI GTAC 2 cut(s) 190, 335
RsaNI GTAC 2 cut(s) 189, 334
RseI CAYNNNNRTG 2 cut(s) 353, 593
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 6 cut(s) 13, 168, 432, 441, 578, 581
Sau96I GGNCC 1 cut(s) 585
SchI GAGTC 1 cut(s) 217
SetI ASST 5 cut(s) 201, 234, 238, 317, 370
SfaNI GCATC 2 cut(s) 443, 544
SfcI CTRYAG 1 cut(s) 165
SmiMI CAYNNNNRTG 2 cut(s) 353, 593
SmlI CTYRAG 2 cut(s) 122, 227
SmoI CTYRAG 2 cut(s) 122, 227
SphI GCATGC 1 cut(s) 411
Sse9I AATT 2 cut(s) 73, 507
SsiI CCGC 4 cut(s) 109, 478, 489, 583
SspMI CTAG 2 cut(s) 38, 251
StyI CCWWGG 2 cut(s) 139, 326
TaaI ACNGT 2 cut(s) 280, 469
TaqI TCGA 2 cut(s) 71, 99
TasI AATT 2 cut(s) 73, 507
TatI WGTACW 2 cut(s) 188, 333
TfiI GAWTC 1 cut(s) 457
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TscAI CASTG 1 cut(s) 285
TseI GCWGC 6 cut(s) 12, 167, 431, 440, 577, 580
TspDTI ATGAA 3 cut(s) 51, 371, 484
TspRI CASTG 1 cut(s) 285
Vha464I CTTAAG 1 cut(s) 122
XapI RAATTY 1 cut(s) 73
XceI RCATGY 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 464
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 2 cut(s) 38, 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.