MD09G1242900.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011237
Physical Location & Seq
Forward (+)
30993756 .. 30996545
2790 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1242900.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGGATTCAACGGCTATATCGACATCAACGACAACACCCCCTTATCATCTTCATTCTCGGTCCGAGCCGCCGTCCCGAGCCAAGTCAGCCTCACGCCTTGCCCAGTGCGAGCATGTCCCTCATCTCTCTCTCGGCCTCATAAAATCATCACCCAAGATGACTCCATCGCCTTCCACTCATTCCCTCAAATCTTCCGAGTCGCTACCTCTTCGCGAGCTACTGCTTCTTTCGCCTTCCCCTTTACGAAAAAGATCCAAGACCCGTCTCACGGATCGCCTTGAAATGGCTGACGATCCTGTAGTCGAGGTTCCAGGGCTGCGCCGTAAGTGCAAAAGCAGAGGCTCGCAGATGGGCCTACTGGGTGCCTCGCCGAGGAACCGGAGGTCGAGGAGGAGGTCGGAGAATGAGGTTCGAGAAGAGAAAGTCTCGGGCTTAGTGGAGGAGGTTGCGAAGCCAAGAAAAAGGCGGTCCAAGAAGGAAAAGCTGGACTTGGTTCTCAGTTTGCCATCCCCAAGTTCATCATCAATCGATGATGAGTATCGGGGTAGTCTTGATCGAATCGGGCAGCTCATGACTGATCTGATTATGTGGAGAGATGTTGCAAGGTCGAGCCTTTGGTTTGGTTTGGGGTCGCTCTTCTTCCTTTCCTCTTGCTTTGCCAAAGGAATCAACTTTAGCATTTTCTCAGCGATTTCCCAAATTGGACTTTTGTTTTTGGGTGCTTCATTTGTCTCCAATTCAATATGTCAAAGAAATAATACCGAAAAGAAGTGTGATCTATTACTCAAAGAAGGTGACATTCTTGGTGTGGCCAAAAGGATACTACCTGCTGTAAATCTTGCCCTATCAAAGACGAGAGCGCTTTTCTCCGGAGAGCCATCCATGACCCTCAAGGTAGCTCCATTCTTTCTTCTGGGAGCAGAGTATGGGCATCTTATAACATTTTGGAGGCTCTGTATGCTTGCTTTTTTTATCAGCTTCACAATCCCAAAACTATACTCATGCTACTCCATTCAAATCAACCAAAAAGTTGACTGCTTGAAATGGTGGGTGTTGGAAGCATGGGGGGCTTGCTCACACAAAAGGATTGTAGCAGCATCAGCAGCCACAGCTTTTTGGAATCTGTCCTCTGTCAAAACCCGTATTTTCACAGCATTTATATCTCTTGTAATTCTTCGATACTGCCGGCAACATATGGTGCAACCGATGGAGACTGAGGAAGCAGAAGTGGAGCAGGAGCAGCATCACAAGGCATTAGCAGTAGCTGGGGTGGTGGGAGAAGTGGACGCAGACAGAAAACAAGAGCAGAAACAGGCATTAGTAGTTGCAGAAGTGGCAAGCAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

450

Amino Acids

50.11

Weight (kDa)

9.68

Isoelectric Point (pI)

57.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 192 - 345 1.5e-30 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011237)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20590 AT2G20590 AT2G20590
fragaria_vesca FvH4_6g26620 FvH4_6g26620 FvH4_6g26620
malus_domestica MD09G1242900.v1.1
prunus_persica Prupe.3G132900_v2.0.a1 Prupe.3G133200_v2.0.a1
pyrus_communis pycom09g16120
rosa_chinensis RchiOBHm_Chr2g0127351
rosa_laevigata RLG00000018965
rosa_multiflora Rmu_ssc0000018.1_g000044
rosa_roxburghii Rroxscaffold_2G00116360
rosa_rugosa Rorug02G0275000
rosa_samantha Rh2AG326100 Rh2BG335600 Rh2CG313800 Rh2DG353300
rosa_wichuraiana Rw2G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 938
Acc36I ACCTGC 1 cut(s) 835
AccB1I GGYRCC 1 cut(s) 362
AccII CGCG 1 cut(s) 213
AccIII TCCGGA 1 cut(s) 869
AciI CCGC 2 cut(s) 68, 466
AclWI GGATC 3 cut(s) 246, 279, 287
AcoI YGGCCR 1 cut(s) 810
AfeI AGCGCT 1 cut(s) 861
AfiI CCNNNNNNNGG 3 cut(s) 268, 283, 372
AgsI TTSAA 5 cut(s) 9, 281, 741, 1016, 1042
AjnI CCWGG 1 cut(s) 310
AluBI AGCT 7 cut(s) 217, 484, 568, 899, 978, 1112, 1265
AluI AGCT 7 cut(s) 217, 484, 568, 899, 978, 1112, 1265
Alw26I GTCTC 4 cut(s) 269, 430, 736, 1205
AlwI GGATC 3 cut(s) 246, 279, 287
AlwNI CAGNNNCTG 1 cut(s) 1265
Ama87I CYCGRG 2 cut(s) 75, 427
Aor13HI TCCGGA 1 cut(s) 869
Aor51HI AGCGCT 1 cut(s) 861
AoxI GGCC 3 cut(s) 133, 352, 810
ApeKI GCWGC 5 cut(s) 316, 565, 1094, 1103, 1240
AspLEI GCGC 2 cut(s) 321, 862
AspS9I GGNCC 3 cut(s) 60, 352, 468
AsuHPI GGTGA 2 cut(s) 141, 806
AvaI CYCGRG 2 cut(s) 75, 427
AvaII GGWCC 2 cut(s) 60, 468
BalI TGGCCA 1 cut(s) 812
BanI GGYRCC 1 cut(s) 362
BbvI GCAGC 5 cut(s) 303, 577, 1106, 1115, 1252
BccI CCATC 5 cut(s) 172, 343, 514, 886, 1201
BceAI ACGGC 3 cut(s) 27, 55, 306
BciT130I CCWGG 1 cut(s) 312
BciVI GTATCC 1 cut(s) 813
BcoDI GTCTC 4 cut(s) 269, 430, 736, 1205
BfmI CTRYAG 1 cut(s) 297
BfoI RGCGCY 1 cut(s) 863
BfuAI ACCTGC 1 cut(s) 835
BfuI GTATCC 1 cut(s) 813
BisI GCNGC 6 cut(s) 68, 317, 566, 1095, 1104, 1241
BlsI GCNGC 6 cut(s) 69, 318, 567, 1096, 1105, 1242
Bme1390I CCNGG 1 cut(s) 312
Bme18I GGWCC 2 cut(s) 60, 468
BmeT110I CYCGRG 2 cut(s) 75, 427
BmgT120I GGNCC 3 cut(s) 60, 352, 468
BmiI GGNNCC 3 cut(s) 309, 364, 377
BmrFI CCNGG 1 cut(s) 312
BmrI ACTGGG 2 cut(s) 97, 368
BmsI GCATC 3 cut(s) 940, 1106, 1252
BmuI ACTGGG 2 cut(s) 97, 368
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BpuEI CTTGAG 1 cut(s) 875
Bsa29I ATCGAT 1 cut(s) 528
BsaBI GATNNNNATC 1 cut(s) 537
BsaJI CCNNGG 2 cut(s) 311, 371
BsaWI WCCGGW 2 cut(s) 378, 869
BsaXI ACNNNNNCTCC 2 cut(s) 940, 970
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 283, 372
Bse118I RCCGGY 1 cut(s) 1185
Bse1I ACTGG 2 cut(s) 103, 363
Bse8I GATNNNNATC 1 cut(s) 537
BseAI TCCGGA 1 cut(s) 869
BseBI CCWGG 1 cut(s) 312
BseCI ATCGAT 1 cut(s) 528
BseDI CCNNGG 2 cut(s) 311, 371
BseGI GGATG 2 cut(s) 506, 878
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 3 cut(s) 268, 283, 372
BseMII CTCAG 3 cut(s) 511, 699, 1206
BseNI ACTGG 2 cut(s) 103, 363
BseRI GAGGAG 3 cut(s) 403, 406, 455
BseXI GCAGC 5 cut(s) 303, 577, 1106, 1115, 1252
BseYI CCCAGC 1 cut(s) 1265
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 3 cut(s) 135, 354, 812
BshNI GGYRCC 1 cut(s) 362
BshVI ATCGAT 1 cut(s) 528
BsiHKCI CYCGRG 2 cut(s) 75, 427
BsiSI CCGG 3 cut(s) 379, 870, 1186
BslFI GGGAC 2 cut(s) 58, 101
BslI CCNNNNNNNGG 3 cut(s) 268, 283, 372
BsmAI GTCTC 4 cut(s) 269, 430, 736, 1205
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 2 cut(s) 58, 101
BsnI GGCC 3 cut(s) 135, 354, 812
BsoBI CYCGRG 2 cut(s) 75, 427
Bsp13I TCCGGA 1 cut(s) 869
Bsp143I GATC 6 cut(s) 251, 271, 292, 553, 577, 775
Bsp68I TCGCGA 1 cut(s) 213
BspACI CCGC 2 cut(s) 68, 466
BspANI GGCC 3 cut(s) 135, 354, 812
BspCNI CTCAG 3 cut(s) 510, 698, 1207
BspDI ATCGAT 1 cut(s) 528
BspEI TCCGGA 1 cut(s) 869
BspFNI CGCG 1 cut(s) 213
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 3 cut(s) 309, 364, 377
BspMI ACCTGC 1 cut(s) 835
BspPI GGATC 3 cut(s) 246, 279, 287
BspQI GCTCTTC 1 cut(s) 641
BspT107I GGYRCC 1 cut(s) 362
BsrFI RCCGGY 1 cut(s) 1185
BsrI ACTGG 2 cut(s) 103, 363
BssAI RCCGGY 1 cut(s) 1185
BssECI CCNNGG 2 cut(s) 311, 371
BssMI GATC 6 cut(s) 251, 271, 292, 553, 577, 775
Bst2UI CCWGG 1 cut(s) 312
Bst6I CTCTTC 3 cut(s) 213, 411, 641
BstC8I GCNNGC 7 cut(s) 110, 215, 344, 963, 1072, 1187, 1339
BstDEI CTNAG 4 cut(s) 433, 497, 685, 1215
BstENI CCTNNNNNAGG 1 cut(s) 370
BstF5I GGATG 2 cut(s) 506, 878
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 863
BstHHI GCGC 2 cut(s) 321, 862
BstKTI GATC 6 cut(s) 254, 274, 295, 556, 580, 778
BstMAI GTCTC 4 cut(s) 269, 430, 736, 1205
BstMBI GATC 6 cut(s) 251, 271, 292, 553, 577, 775
BstNI CCWGG 1 cut(s) 312
BstNSI RCATGY 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 297
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 5 cut(s) 303, 577, 1106, 1115, 1252
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
Bsu15I ATCGAT 1 cut(s) 528
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 3 cut(s) 135, 354, 812
BsuTUI ATCGAT 1 cut(s) 528
BtgZI GCGATG 1 cut(s) 150
BtsCI GGATG 2 cut(s) 506, 878
BtsIMutI CAGTG 1 cut(s) 110
BtuMI TCGCGA 1 cut(s) 213
BveI ACCTGC 1 cut(s) 835
Cac8I GCNNGC 7 cut(s) 110, 215, 344, 963, 1072, 1187, 1339
CaiI CAGNNNCTG 1 cut(s) 1265
CciI TCATGA 1 cut(s) 570
CfoI GCGC 2 cut(s) 321, 862
Cfr10I RCCGGY 1 cut(s) 1185
Cfr13I GGNCC 3 cut(s) 60, 352, 468
ClaI ATCGAT 1 cut(s) 528
CpoI CGGWCCG 1 cut(s) 60
CseI GACGC 1 cut(s) 1295
CspI CGGWCCG 1 cut(s) 60
CviAII CATG 5 cut(s) 113, 571, 883, 1002, 1062
DdeI CTNAG 4 cut(s) 433, 497, 685, 1215
DpnI GATC 6 cut(s) 253, 273, 294, 555, 579, 777
DpnII GATC 6 cut(s) 251, 271, 292, 553, 577, 775
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 3 cut(s) 213, 411, 641
EarI CTCTTC 3 cut(s) 213, 411, 641
Eco47I GGWCC 2 cut(s) 60, 468
Eco47III AGCGCT 1 cut(s) 861
Eco88I CYCGRG 2 cut(s) 75, 427
EcoNI CCTNNNNNAGG 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 5 cut(s) 116, 574, 886, 1005, 1065
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FaqI GGGAC 2 cut(s) 58, 101
FatI CATG 5 cut(s) 112, 570, 882, 1001, 1061
FauNDI CATATG 1 cut(s) 1194
Fnu4HI GCNGC 6 cut(s) 68, 317, 566, 1095, 1104, 1241
FokI GGATG 2 cut(s) 493, 865
Fsp4HI GCNGC 6 cut(s) 68, 317, 566, 1095, 1104, 1241
GlaI GCGC 2 cut(s) 320, 861
GluI GCNGC 6 cut(s) 68, 317, 566, 1095, 1104, 1241
GsaI CCCAGC 1 cut(s) 1269
HaeII RGCGCY 1 cut(s) 863
HaeIII GGCC 3 cut(s) 135, 354, 812
HapII CCGG 3 cut(s) 379, 870, 1186
HgaI GACGC 1 cut(s) 1295
HhaI GCGC 2 cut(s) 321, 862
Hin1II CATG 5 cut(s) 116, 574, 886, 1005, 1065
Hin6I GCGC 2 cut(s) 319, 860
HinP1I GCGC 2 cut(s) 319, 860
HincII GTYRAC 1 cut(s) 1033
HindII GTYRAC 1 cut(s) 1033
HinfI GANTC 6 cut(s) 5, 160, 197, 558, 666, 1120
HpaII CCGG 3 cut(s) 379, 870, 1186
HphI GGTGA 2 cut(s) 141, 806
Hpy166II GTNNAC 2 cut(s) 1033, 1285
Hpy188I TCNGA 4 cut(s) 64, 196, 400, 582
Hpy188III TCNNGA 6 cut(s) 75, 212, 413, 551, 571, 870
Hpy8I GTNNAC 2 cut(s) 1033, 1285
HpyAV CCTTC 4 cut(s) 180, 243, 469, 785
HpyCH4V TGCA 4 cut(s) 330, 602, 1201, 1328
HpyF3I CTNAG 4 cut(s) 433, 497, 685, 1215
Hsp92II CATG 5 cut(s) 116, 574, 886, 1005, 1065
HspAI GCGC 2 cut(s) 319, 860
Kpn2I TCCGGA 1 cut(s) 869
KroI GCCGGC 1 cut(s) 1185
KroNI GCCGGC 1 cut(s) 1187
Kzo9I GATC 6 cut(s) 251, 271, 292, 553, 577, 775
LguI GCTCTTC 1 cut(s) 641
LmnI GCTCC 4 cut(s) 904, 917, 1231, 1237
Lsp1109I GCAGC 5 cut(s) 303, 577, 1106, 1115, 1252
LweI GCATC 3 cut(s) 940, 1106, 1252
MaeIII GTNAC 1 cut(s) 794
MalI GATC 6 cut(s) 253, 273, 294, 555, 579, 777
MboI GATC 6 cut(s) 251, 271, 292, 553, 577, 775
MboII GAAGA 8 cut(s) 41, 183, 200, 428, 628, 631, 902, 1166
MflI RGATCY 1 cut(s) 251
MlsI TGGCCA 1 cut(s) 812
MluCI AATT 3 cut(s) 699, 736, 1170
MluNI TGGCCA 1 cut(s) 812
MlyI GAGTC 2 cut(s) 154, 206
MmeI TCCRAC 2 cut(s) 378, 1035
Mox20I TGGCCA 1 cut(s) 812
MroI TCCGGA 1 cut(s) 869
MroNI GCCGGC 1 cut(s) 1185
MscI TGGCCA 1 cut(s) 812
Msp20I TGGCCA 1 cut(s) 812
MspI CCGG 3 cut(s) 379, 870, 1186
MspR9I CCNGG 1 cut(s) 312
MvaI CCWGG 1 cut(s) 312
MvnI CGCG 1 cut(s) 213
NaeI GCCGGC 1 cut(s) 1187
NdeI CATATG 1 cut(s) 1194
NdeII GATC 6 cut(s) 251, 271, 292, 553, 577, 775
NgoMIV GCCGGC 1 cut(s) 1185
NlaIII CATG 5 cut(s) 116, 574, 886, 1005, 1065
NlaIV GGNNCC 3 cut(s) 309, 364, 377
NmeAIII GCCGAG 2 cut(s) 111, 396
NmuCI GTSAC 1 cut(s) 794
NruI TCGCGA 1 cut(s) 213
NspI RCATGY 1 cut(s) 116
PagI TCATGA 1 cut(s) 570
PciSI GCTCTTC 1 cut(s) 641
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 26
PdiI GCCGGC 1 cut(s) 1187
PfeI GAWTC 4 cut(s) 5, 558, 666, 1120
PkrI GCNGC 6 cut(s) 69, 318, 567, 1096, 1105, 1242
PleI GAGTC 2 cut(s) 154, 205
PpsI GAGTC 2 cut(s) 154, 205
PsiI TTATAA 1 cut(s) 938
Psp6I CCWGG 1 cut(s) 310
PspFI CCCAGC 1 cut(s) 1265
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 3 cut(s) 309, 364, 377
PspPI GGNCC 3 cut(s) 60, 352, 468
PsrI GAACNNNNNNTAC 2 cut(s) 291, 323
PstNI CAGNNNCTG 1 cut(s) 1265
PsuI RGATCY 1 cut(s) 251
RruI TCGCGA 1 cut(s) 213
Rsr2I CGGWCCG 1 cut(s) 60
RsrII CGGWCCG 1 cut(s) 60
SapI GCTCTTC 1 cut(s) 641
SatI GCNGC 6 cut(s) 68, 317, 566, 1095, 1104, 1241
Sau3AI GATC 6 cut(s) 251, 271, 292, 553, 577, 775
Sau96I GGNCC 3 cut(s) 60, 352, 468
SchI GAGTC 2 cut(s) 154, 206
ScrFI CCNGG 1 cut(s) 312
SfaNI GCATC 3 cut(s) 940, 1106, 1252
SfcI CTRYAG 1 cut(s) 297
SinI GGWCC 2 cut(s) 60, 468
SmlI CTYRAG 1 cut(s) 890
SmoI CTYRAG 1 cut(s) 890
Sse9I AATT 3 cut(s) 699, 736, 1170
SsiI CCGC 2 cut(s) 68, 466
StyD4I CCNGG 1 cut(s) 310
TaqI TCGA 8 cut(s) 20, 303, 386, 412, 528, 556, 608, 1177
TaqII GACCGA 1 cut(s) 48
TasI AATT 3 cut(s) 699, 736, 1170
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 4 cut(s) 5, 558, 666, 1120
TscAI CASTG 1 cut(s) 110
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 5 cut(s) 316, 565, 1094, 1103, 1240
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 3 cut(s) 41, 507, 714
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 1 cut(s) 110
VpaK11BI GGWCC 2 cut(s) 60, 468
XagI CCTNNNNNAGG 1 cut(s) 370
XceI RCATGY 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.