RLG00000018965

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011237
Physical Location & Seq
Forward (+)
43720338 .. 43722555
2218 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018965

Sequence Viewer

Length: 1314 bp
ATGGATTCGACAGCTTCACTGCCTCCCTATCGTCACCATTCTCGTTCGGAGCCACCGTCCCGAACCAAAACGGCGTCACGGCTCGCCCAATCGGAGAAGGTCCCTCATCTCTCTCTGGGTCTCATTCAATCACCTTCACCTCCGTCTCGCAAATCTTCGGCTTCTCTCCCTCTACACGAGCTGCTGCTACTCTCGCCTTCGCCTAATCCAAGAAGGTCCAAGACCCGTCTCGCCGATCGGTTTGAAATGCCCGATGAGCCGGTGGCGGAACCGGCAGGCCCACGACGGAGGTGCAAGAGCAGCAGAGGCTCCCAGATGGGCTCTCTGGGATGTGCCTCGCCCAGGAACACCAGAAGGTCCAGGAGGAGATCGGAGGTTGAGATTCGAGAAGATAAGGATTTGGGTTTGGGGGAAGAGACTGTCAAGGTCAGGAAAAGAAGGTCCAAGAAGGACAGGCTGAGCTTGGTGCCTTGTGTGGTGCTGCCCTCCCCAACTGCCTCTACAATGGATGATGACGATGGGGGTCGTCTGGAGCGAATTGGGCAGCTATTGAATGATCTGATCATGTGGAGAGATGTAGCAAGATCGACCCTTTGGTTTGGTTTGGGCTCTCTGTTCTTCTTGTCTTCTTGCTTTGCCAAAGGAGTGAACTTTAGCGTTTTCTCGGCAATTTCCCAACTAGGACTTCTGTTCTTGGGTGCTTCATTTGTCTCGAATTCAATCTCCCAAAGAAATGGTATCGAAAAGAAGCGGGATTACTTGCTTAAGGAAAATGATATCCTTGGTGTGACCAAAATGATACTCCCTGCAGCAAATCTTGCAATATCAAGTACAAGGAAGCTTTTCTCAGGAGAACCATCTATGACTCTCAAGGTAGCTCCATTCTTTCTTCTAGGAGCCGAGTATGGACATCATATAACATTGTGGAGGCTCTCTGTGCTTGCATTCTTTATCAGCTTCACAATCCCAAGGCTGTACTCATGCTACTCCATTCAAATGAACCAAAGAGCTGAGTGCTTGAAATGGTGCGTGTTGGAAGCATGGGGAGCATGCTCTCACAAAAAGATTGTAGCAGCATCAGCAGCCACAGCATTCTGGAATCTGTCTACGGTGAAAACCCGCATATTCACCGCATTCATATCCCTCGTAATTCTTCGTTACTGCCGACAACACATGGTGCAAGATAGGGAAGCAGATGCAGAAGCAGAACAGGAGGAGCAGCAGCAGGCCATAGTAGTGGCAGAAGCAGAAGGAAAACAGGAGCAGCAGCAAGAGCAGGCATTAGTAGTGGCCGAAGTGGGCAAGCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

438

Amino Acids

48.74

Weight (kDa)

9.62

Isoelectric Point (pI)

66.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 185 - 338 1.2e-26 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011237)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20590 AT2G20590 AT2G20590
fragaria_vesca FvH4_6g26620 FvH4_6g26620 FvH4_6g26620
malus_domestica MD09G1242900.v1.1
prunus_persica Prupe.3G132900_v2.0.a1 Prupe.3G133200_v2.0.a1
pyrus_communis pycom09g16120
rosa_chinensis RchiOBHm_Chr2g0127351
rosa_laevigata RLG00000018965
rosa_multiflora Rmu_ssc0000018.1_g000044
rosa_roxburghii Rroxscaffold_2G00116360
rosa_rugosa Rorug02G0275000
rosa_samantha Rh2AG326100 Rh2BG335600 Rh2CG313800 Rh2DG353300
rosa_wichuraiana Rw2G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 466
AccI GTMKAC 1 cut(s) 1106
AciI CCGC 4 cut(s) 266, 751, 1120, 1131
AcoI YGGCCR 1 cut(s) 1290
AcsI RAATTY 1 cut(s) 715
AcyI GRCGYC 1 cut(s) 74
AdeI CACNNNGTG 1 cut(s) 1177
AfaI GTAC 2 cut(s) 832, 977
AfiI CCNNNNNNNGG 1 cut(s) 342
AflII CTTAAG 1 cut(s) 764
AgsI TTSAA 6 cut(s) 128, 245, 553, 720, 995, 1021
AjnI CCWGG 2 cut(s) 341, 359
AjuI GAANNNNNNNTTGG 2 cut(s) 669, 701
AluBI AGCT 8 cut(s) 14, 181, 462, 547, 841, 878, 957, 1010
AluI AGCT 8 cut(s) 14, 181, 462, 547, 841, 878, 957, 1010
Alw26I GTCTC 5 cut(s) 125, 150, 233, 410, 715
AoxI GGCC 3 cut(s) 277, 1227, 1290
ApoI RAATTY 1 cut(s) 715
Asp700I GAANNNNTTC 1 cut(s) 842
AspS9I GGNCC 5 cut(s) 100, 216, 278, 357, 441
AsuHPI GGTGA 5 cut(s) 26, 123, 129, 1120, 1123
AvaII GGWCC 4 cut(s) 100, 216, 357, 441
BanI GGYRCC 1 cut(s) 466
BanII GRGCYC 2 cut(s) 323, 611
BarI GAAGNNNNNNTAC 2 cut(s) 740, 772
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 618
BccI CCATC 3 cut(s) 310, 512, 865
BceAI ACGGC 2 cut(s) 87, 95
BcgI CGANNNNNNTGC 2 cut(s) 273, 307
BciT130I CCWGG 2 cut(s) 343, 361
BclI TGATCA 1 cut(s) 561
BcoDI GTCTC 5 cut(s) 125, 150, 233, 410, 715
BfaI CTAG 2 cut(s) 680, 893
BfmI CTRYAG 1 cut(s) 807
BfrI CTTAAG 1 cut(s) 764
BlpI GCTNAGC 1 cut(s) 458
Bme1390I CCNGG 2 cut(s) 343, 361
Bme18I GGWCC 4 cut(s) 100, 216, 357, 441
BmgT120I GGNCC 5 cut(s) 100, 216, 278, 357, 441
BmiI GGNNCC 6 cut(s) 51, 102, 270, 310, 468, 898
BmrFI CCNGG 2 cut(s) 343, 361
BmsI GCATC 2 cut(s) 1085, 1186
BpiI GAAGAC 1 cut(s) 618
BpmI CTGGAG 1 cut(s) 551
Bpu1102I GCTNAGC 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 854
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 3 cut(s) 341, 781, 968
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 1 cut(s) 342
Bse118I RCCGGY 2 cut(s) 259, 271
BseBI CCWGG 2 cut(s) 343, 361
BseDI CCNNGG 3 cut(s) 341, 781, 968
BseGI GGATG 2 cut(s) 335, 514
BseLI CCNNNNNNNGG 1 cut(s) 342
BseMII CTCAG 3 cut(s) 449, 861, 1002
BseRI GAGGAG 2 cut(s) 379, 1229
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 3 cut(s) 279, 1229, 1292
BshNI GGYRCC 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 238
BsiSI CCGG 2 cut(s) 260, 272
BslFI GGGAC 2 cut(s) 43, 86
BslI CCNNNNNNNGG 1 cut(s) 342
BsmAI GTCTC 5 cut(s) 125, 150, 233, 410, 715
BsmBI CGTCTC 2 cut(s) 150, 233
BsmFI GGGAC 2 cut(s) 43, 86
BsmI GAATGC 3 cut(s) 944, 1091, 1133
BsnI GGCC 3 cut(s) 279, 1229, 1292
Bso31I GGTCTC 1 cut(s) 125
Bsp1286I GDGCHC 2 cut(s) 323, 611
Bsp143I GATC 5 cut(s) 235, 368, 556, 561, 584
Bsp1720I GCTNAGC 1 cut(s) 458
BspACI CCGC 4 cut(s) 266, 751, 1120, 1131
BspANI GGCC 3 cut(s) 279, 1229, 1292
BspCNI CTCAG 3 cut(s) 450, 860, 1003
BspLI GGNNCC 6 cut(s) 51, 102, 270, 310, 468, 898
BspMAI CTGCAG 1 cut(s) 811
BspT107I GGYRCC 1 cut(s) 466
BspTI CTTAAG 1 cut(s) 764
BspTNI GGTCTC 1 cut(s) 125
BsrFI RCCGGY 2 cut(s) 259, 271
BssAI RCCGGY 2 cut(s) 259, 271
BssECI CCNNGG 3 cut(s) 341, 781, 968
BssMI GATC 5 cut(s) 235, 368, 556, 561, 584
BssNI GRCGYC 1 cut(s) 74
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 2 cut(s) 781, 968
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 2 cut(s) 343, 361
Bst4CI ACNGT 3 cut(s) 57, 421, 1111
Bst6I CTCTTC 1 cut(s) 408
BstACI GRCGYC 1 cut(s) 74
BstAFI CTTAAG 1 cut(s) 764
BstAPI GCANNNNNTGC 1 cut(s) 818
BstC8I GCNNGC 7 cut(s) 84, 277, 942, 1051, 1227, 1278, 1304
BstDEI CTNAG 3 cut(s) 458, 847, 1011
BstENI CCTNNNNNAGG 1 cut(s) 340
BstF5I GGATG 2 cut(s) 335, 514
BstKTI GATC 5 cut(s) 238, 371, 559, 564, 587
BstMAI GTCTC 5 cut(s) 125, 150, 233, 410, 715
BstMBI GATC 5 cut(s) 235, 368, 556, 561, 584
BstMCI CGRYCG 1 cut(s) 238
BstNI CCWGG 2 cut(s) 343, 361
BstNSI RCATGY 1 cut(s) 1053
BstSCI CCNGG 2 cut(s) 341, 359
BstSFI CTRYAG 1 cut(s) 807
BstV2I GAAGAC 1 cut(s) 618
BstXI CCANNNNNNTGG 2 cut(s) 734, 1237
BsuRI GGCC 3 cut(s) 279, 1229, 1292
BtsCI GGATG 2 cut(s) 335, 514
BtsI GCAGTG 1 cut(s) 17
BtsIMutI CAGTG 1 cut(s) 17
Cac8I GCNNGC 7 cut(s) 84, 277, 942, 1051, 1227, 1278, 1304
Cfr10I RCCGGY 2 cut(s) 259, 271
Cfr13I GGNCC 5 cut(s) 100, 216, 278, 357, 441
CseI GACGC 1 cut(s) 63
Csp6I GTAC 2 cut(s) 831, 976
CviAII CATG 5 cut(s) 565, 981, 1041, 1050, 1174
CviQI GTAC 2 cut(s) 831, 976
DdeI CTNAG 3 cut(s) 458, 847, 1011
DpnI GATC 5 cut(s) 237, 370, 558, 563, 586
DpnII GATC 5 cut(s) 235, 368, 556, 561, 584
DraIII CACNNNGTG 1 cut(s) 1177
EaeI YGGCCR 1 cut(s) 1290
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 781, 968
Eco24I GRGCYC 2 cut(s) 323, 611
Eco31I GGTCTC 1 cut(s) 125
Eco32I GATATC 1 cut(s) 778
Eco47I GGWCC 4 cut(s) 100, 216, 357, 441
EcoNI CCTNNNNNAGG 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 715
EcoRII CCWGG 2 cut(s) 341, 359
EcoRV GATATC 1 cut(s) 778
EcoT14I CCWWGG 2 cut(s) 781, 968
EcoT38I GRGCYC 2 cut(s) 323, 611
ErhI CCWWGG 2 cut(s) 781, 968
Esp3I CGTCTC 2 cut(s) 150, 233
FaeI CATG 5 cut(s) 568, 984, 1044, 1053, 1177
FaqI GGGAC 2 cut(s) 43, 86
FatI CATG 5 cut(s) 564, 980, 1040, 1049, 1173
FauI CCCGC 2 cut(s) 744, 1127
FbaI TGATCA 1 cut(s) 561
FblI GTMKAC 1 cut(s) 1106
FokI GGATG 2 cut(s) 342, 521
FriOI GRGCYC 2 cut(s) 323, 611
FspBI CTAG 2 cut(s) 680, 893
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 3 cut(s) 279, 1229, 1292
HapII CCGG 2 cut(s) 260, 272
HgaI GACGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 5 cut(s) 568, 984, 1044, 1053, 1177
HindIII AAGCTT 1 cut(s) 839
HinfI GANTC 4 cut(s) 5, 382, 865, 1099
HpaII CCGG 2 cut(s) 260, 272
HphI GGTGA 5 cut(s) 26, 123, 129, 1120, 1123
Hpy166II GTNNAC 2 cut(s) 649, 1107
Hpy188I TCNGA 4 cut(s) 49, 94, 373, 561
Hpy188III TCNNGA 7 cut(s) 60, 386, 430, 530, 712, 849, 1096
Hpy8I GTNNAC 2 cut(s) 649, 1107
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 8 cut(s) 91, 144, 207, 207, 348, 432, 442, 1244
HpyCH4III ACNGT 3 cut(s) 57, 421, 1111
HpyCH4V TGCA 6 cut(s) 294, 809, 821, 944, 1180, 1199
HpyF3I CTNAG 3 cut(s) 458, 847, 1011
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 5 cut(s) 568, 984, 1044, 1053, 1177
Ksp22I TGATCA 1 cut(s) 561
Kzo9I GATC 5 cut(s) 235, 368, 556, 561, 584
LmnI GCTCC 8 cut(s) 49, 314, 532, 883, 896, 1046, 1216, 1261
LweI GCATC 2 cut(s) 1085, 1186
MaeI CTAG 2 cut(s) 680, 893
MaeIII GTNAC 4 cut(s) 32, 75, 787, 1157
MalI GATC 5 cut(s) 237, 370, 558, 563, 586
MboI GATC 5 cut(s) 235, 368, 556, 561, 584
MboII GAAGA 7 cut(s) 147, 401, 425, 610, 618, 881, 1145
MhlI GDGCHC 2 cut(s) 323, 611
MluCI AATT 4 cut(s) 537, 669, 715, 1149
MlyI GAGTC 1 cut(s) 859
MmeI TCCRAC 1 cut(s) 1014
MroXI GAANNNNTTC 1 cut(s) 842
MseI TTAA 1 cut(s) 765
MslI CAYNNNNRTG 2 cut(s) 995, 1235
MspCI CTTAAG 1 cut(s) 764
MspI CCGG 2 cut(s) 260, 272
MspR9I CCNGG 2 cut(s) 343, 361
Mva1269I GAATGC 3 cut(s) 944, 1091, 1133
MvaI CCWGG 2 cut(s) 343, 361
NdeII GATC 5 cut(s) 235, 368, 556, 561, 584
NlaIII CATG 5 cut(s) 568, 984, 1044, 1053, 1177
NlaIV GGNNCC 6 cut(s) 51, 102, 270, 310, 468, 898
NmeAIII GCCGAG 2 cut(s) 644, 925
NmuCI GTSAC 3 cut(s) 32, 75, 787
NspI RCATGY 1 cut(s) 1053
PaeI GCATGC 1 cut(s) 1053
PcsI WCGNNNNNNNCGW 3 cut(s) 53, 532, 1162
PctI GAATGC 3 cut(s) 944, 1091, 1133
PdmI GAANNNNTTC 1 cut(s) 842
PfeI GAWTC 3 cut(s) 5, 382, 1099
PfoI TCCNGGA 1 cut(s) 359
Ple19I CGATCG 1 cut(s) 238
PleI GAGTC 1 cut(s) 859
PpsI GAGTC 1 cut(s) 859
PpuMI RGGWCCY 1 cut(s) 100
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 341, 359
PspGI CCWGG 2 cut(s) 341, 359
PspN4I GGNNCC 6 cut(s) 51, 102, 270, 310, 468, 898
PspPI GGNCC 5 cut(s) 100, 216, 278, 357, 441
PspPPI RGGWCCY 1 cut(s) 100
PstI CTGCAG 1 cut(s) 811
PvuI CGATCG 1 cut(s) 238
RsaI GTAC 2 cut(s) 832, 977
RsaNI GTAC 2 cut(s) 831, 976
RseI CAYNNNNRTG 2 cut(s) 995, 1235
SaqAI TTAA 1 cut(s) 765
Sau3AI GATC 5 cut(s) 235, 368, 556, 561, 584
Sau96I GGNCC 5 cut(s) 100, 216, 278, 357, 441
SchI GAGTC 1 cut(s) 859
ScrFI CCNGG 2 cut(s) 343, 361
SduI GDGCHC 2 cut(s) 323, 611
SfaNI GCATC 2 cut(s) 1085, 1186
SfcI CTRYAG 1 cut(s) 807
SinI GGWCC 4 cut(s) 100, 216, 357, 441
SmiMI CAYNNNNRTG 2 cut(s) 995, 1235
SmlI CTYRAG 2 cut(s) 764, 869
SmoI CTYRAG 2 cut(s) 764, 869
SphI GCATGC 1 cut(s) 1053
Sse9I AATT 4 cut(s) 537, 669, 715, 1149
SsiI CCGC 4 cut(s) 266, 751, 1120, 1131
SspMI CTAG 2 cut(s) 680, 893
StyD4I CCNGG 2 cut(s) 341, 359
StyI CCWWGG 2 cut(s) 781, 968
TaaI ACNGT 3 cut(s) 57, 421, 1111
TaqI TCGA 5 cut(s) 8, 385, 587, 713, 741
TasI AATT 4 cut(s) 537, 669, 715, 1149
TatI WGTACW 2 cut(s) 830, 975
TfiI GAWTC 3 cut(s) 5, 382, 1099
Tru1I TTAA 1 cut(s) 765
Tru9I TTAA 1 cut(s) 765
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 3 cut(s) 32, 75, 787
Tsp45I GTSAC 3 cut(s) 32, 75, 787
TspDTI ATGAA 3 cut(s) 693, 1013, 1126
TspGWI ACGGA 2 cut(s) 132, 301
TspRI CASTG 1 cut(s) 24
Vha464I CTTAAG 1 cut(s) 764
VpaK11BI GGWCC 4 cut(s) 100, 216, 357, 441
XagI CCTNNNNNAGG 1 cut(s) 340
XapI RAATTY 1 cut(s) 715
XceI RCATGY 1 cut(s) 1053
XmiI GTMKAC 1 cut(s) 1106
XmnI GAANNNNTTC 1 cut(s) 842
XspI CTAG 2 cut(s) 680, 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.