FvH4_6g26620

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011237
Physical Location & Seq
Forward (+)
20315198 .. 20317879
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26620.t2

Sequence Viewer

Length: 1305 bp
ATGGACTCCACCGCCTCACCGCCTCCCTATCGCCACCATTCTCGCTCCGAGCCTCCCTCCCGAACCAAGTCCGCGTCACGCCTCGCCCAATCGGAGAAGGTCCCACATCTCTCTCTCGCTCTCATCCAATCGCCTTCTCCTCCGTCTCGCAAATCTTCCGCTTCTTCTCTCCCTCTCCACGAGCTGCTGCTACTCTCGCCTTCGCCCAACCCAAGAAGGTCCAAGACCCGCCTCGCCGACCGGTTCGACATGCCGGATGAGCCCGCGGCGGAACCACGGCGGAGGTGCAAGAGCAGCAGAGCCTCCCAGATGGGCTCTCTGGGCTGTGCCTCGCCCAGGAACACCAGAAGGTCCAGGAGGAGATCGGAGGTTGAGAGTCGAGAAGATAAGGATTTGGGTTTGGGAGAAGAGGTTGTGAAACCAATCAGGAAAAGAAGGTCTAAGAAGGACAAGCTCGGTTCGGTCCCTTGTGCTCCTTCCCCAACTTCATCTGCAATGGATGATGAGGATGGGAGTGGTCTGGACCGAATTGGGCAGCTGTTGAATGATCTGATAATGTGGAGAGATGTAGCAAGATCAACCCTTTGGTTTGGTCTGGGCACTCTCTTCTTCTTCTCTTCTTGCTTTACCAAAGGAGTCCACTTCAGTGTCTTCTCGGCCGTTTCCCAACTAGGACTTCTGTTCTTGGGTGCTTCATTTGTCTCGAATTCAATTTCTCAGAGAAATGGTATCGAAAAGAAGCACGATTTCTTGCTCAAGGAAAGTGATATTCTTGGTGTGGCCAAAATGATACTTCCTGCAGCAAATCTTGCAATAACATGTACAAGGAAGCTTTTCTCAGGAGAACCATCTATGACTCTCAAGGTAGTTCCATTCTTTCTTCTAGGAGCAGAATATGGACATCATATAACATTGTGGAGGCTCTCTATGCTTGCCTTTTTTATCAGCTTCACAATCCCAAGGCTGTACTCATGCTACTCCATTCAAATGAACCAAAAAGCTGAGTGCTTGAAATGGAGGGTGTTGGAAGCATGGGGAGCATGCTCTCACAAAAAGATTGTAGCAGCATCAGCAGCCACAGCATTTTGGAATCTATCTTCAGTGAAAACCCGTATATTCACCGCATTCATATCCCTAGTAATTCTTCGTTACTGCCGACAACACATGGTGCAAGTTAGAGAAACAGATGCAGAACCAGAAGAGGAGGAGCAGCAGCAGGCCATGGTGGTGGCAGAAGCAGAAGGAAAACAGGAGCAGCCGCCGGAGCAGGCATTAGTTGTGGCCGAAGTGAGCAAGCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

435

Amino Acids

48.35

Weight (kDa)

9.55

Isoelectric Point (pI)

71.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 22 - 175 8e-28 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011237)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20590 AT2G20590 AT2G20590
fragaria_vesca FvH4_6g26620 FvH4_6g26620 FvH4_6g26620
malus_domestica MD09G1242900.v1.1
prunus_persica Prupe.3G132900_v2.0.a1 Prupe.3G133200_v2.0.a1
pyrus_communis pycom09g16120
rosa_chinensis RchiOBHm_Chr2g0127351
rosa_laevigata RLG00000018965
rosa_multiflora Rmu_ssc0000018.1_g000044
rosa_roxburghii Rroxscaffold_2G00116360
rosa_rugosa Rorug02G0275000
rosa_samantha Rh2AG326100 Rh2BG335600 Rh2CG313800 Rh2DG353300
rosa_wichuraiana Rw2G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 74, 266
AcoI YGGCCR 3 cut(s) 657, 780, 1281
AcsI RAATTY 1 cut(s) 706
AcuI CTGAAG 2 cut(s) 628, 1083
AdeI CACNNNGTG 1 cut(s) 1168
AfaI GTAC 2 cut(s) 823, 968
AfiI CCNNNNNNNGG 1 cut(s) 336
AflIII ACRYGT 1 cut(s) 818
AgeI ACCGGT 1 cut(s) 240
AgsI TTSAA 4 cut(s) 544, 711, 986, 1012
AjnI CCWGG 2 cut(s) 335, 353
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AleI CACNNNNGTG 1 cut(s) 645
AluBI AGCT 6 cut(s) 184, 454, 538, 832, 948, 1001
AluI AGCT 6 cut(s) 184, 454, 538, 832, 948, 1001
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 2 cut(s) 150, 706
AoxI GGCC 4 cut(s) 657, 780, 1218, 1281
ApoI RAATTY 1 cut(s) 706
AsiGI ACCGGT 1 cut(s) 240
Asp700I GAANNNNTTC 1 cut(s) 833
AspS9I GGNCC 5 cut(s) 100, 219, 351, 463, 523
AsuHPI GGTGA 2 cut(s) 9, 1111
AvaII GGWCC 5 cut(s) 100, 219, 351, 463, 523
BaeGI GKGCMC 1 cut(s) 602
BalI TGGCCA 1 cut(s) 782
BanII GRGCYC 2 cut(s) 264, 317
BauI CACGAG 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 643
Bbv12I GWGCWC 1 cut(s) 475
BccI CCATC 3 cut(s) 304, 503, 856
BceAI ACGGC 2 cut(s) 293, 644
BciT130I CCWGG 2 cut(s) 337, 355
BcoDI GTCTC 2 cut(s) 150, 706
BfaI CTAG 3 cut(s) 671, 884, 1136
BfmI CTRYAG 1 cut(s) 798
Bme1390I CCNGG 2 cut(s) 337, 355
Bme18I GGWCC 5 cut(s) 100, 219, 351, 463, 523
BmgT120I GGNCC 5 cut(s) 100, 219, 351, 463, 523
BmiI GGNNCC 3 cut(s) 102, 273, 465
BmrFI CCNGG 2 cut(s) 337, 355
BmsI GCATC 2 cut(s) 1076, 1177
BpiI GAAGAC 1 cut(s) 643
BplI GAGNNNNNCTC 2 cut(s) 41, 73
BpuEI CTTGAG 2 cut(s) 740, 845
BsaJI CCNNGG 5 cut(s) 264, 275, 335, 959, 1221
BsaWI WCCGGW 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bsc4I CCNNNNNNNGG 1 cut(s) 336
Bse118I RCCGGY 1 cut(s) 240
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 2 cut(s) 337, 355
BseDI CCNNGG 5 cut(s) 264, 275, 335, 959, 1221
BseGI GGATG 4 cut(s) 123, 262, 505, 514
BseLI CCNNNNNNNGG 1 cut(s) 336
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 3 cut(s) 731, 852, 993
BseRI GAGGAG 4 cut(s) 129, 373, 1217, 1220
BseSI GKGCMC 1 cut(s) 602
BseX3I CGGCCG 1 cut(s) 657
Bsh1236I CGCG 2 cut(s) 74, 266
Bsh1285I CGRYCG 2 cut(s) 241, 660
BshFI GGCC 4 cut(s) 659, 782, 1220, 1283
BshTI ACCGGT 1 cut(s) 240
BsiEI CGRYCG 2 cut(s) 241, 660
BsiHKAI GWGCWC 1 cut(s) 475
BsiSI CCGG 3 cut(s) 241, 254, 1262
BslFI GGGAC 2 cut(s) 86, 449
BslI CCNNNNNNNGG 1 cut(s) 336
BsmAI GTCTC 2 cut(s) 150, 706
BsmBI CGTCTC 1 cut(s) 150
BsmFI GGGAC 2 cut(s) 86, 449
BsmI GAATGC 1 cut(s) 1124
BsnI GGCC 4 cut(s) 659, 782, 1220, 1283
Bsp1286I GDGCHC 4 cut(s) 264, 317, 475, 602
Bsp1407I TGTACA 1 cut(s) 821
Bsp143I GATC 3 cut(s) 362, 547, 575
Bsp19I CCATGG 1 cut(s) 1221
BspANI GGCC 4 cut(s) 659, 782, 1220, 1283
BspCNI CTCAG 3 cut(s) 730, 851, 994
BspFNI CGCG 2 cut(s) 74, 266
BspLI GGNNCC 3 cut(s) 102, 273, 465
BspMAI CTGCAG 1 cut(s) 802
BsrDI GCAATG 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 240
BsrGI TGTACA 1 cut(s) 821
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 5 cut(s) 264, 275, 335, 959, 1221
BssMI GATC 3 cut(s) 362, 547, 575
BssSI CACGAG 1 cut(s) 179
BssT1I CCWWGG 2 cut(s) 959, 1221
Bst2BI CACGAG 1 cut(s) 179
Bst2UI CCWGG 2 cut(s) 337, 355
Bst6I CTCTTC 4 cut(s) 402, 611, 622, 1194
BstAPI GCANNNNNTGC 1 cut(s) 809
BstAUI TGTACA 1 cut(s) 821
BstC8I GCNNGC 6 cut(s) 264, 933, 1042, 1218, 1269, 1295
BstDEI CTNAG 4 cut(s) 441, 717, 838, 1002
BstDSI CCRYGG 3 cut(s) 264, 275, 1221
BstENI CCTNNNNNAGG 1 cut(s) 334
BstF5I GGATG 4 cut(s) 123, 262, 505, 514
BstFNI CGCG 2 cut(s) 74, 266
BstKTI GATC 3 cut(s) 365, 550, 578
BstMAI GTCTC 2 cut(s) 150, 706
BstMBI GATC 3 cut(s) 362, 547, 575
BstMCI CGRYCG 2 cut(s) 241, 660
BstNI CCWGG 2 cut(s) 337, 355
BstNSI RCATGY 3 cut(s) 253, 822, 1044
BstSCI CCNGG 2 cut(s) 335, 353
BstSFI CTRYAG 1 cut(s) 798
BstSLI GKGCMC 1 cut(s) 602
BstUI CGCG 2 cut(s) 74, 266
BstV2I GAAGAC 1 cut(s) 643
BstXI CCANNNNNNTGG 1 cut(s) 1228
BstZI CGGCCG 1 cut(s) 657
BsuRI GGCC 4 cut(s) 659, 782, 1220, 1283
BtgI CCRYGG 3 cut(s) 264, 275, 1221
BtsCI GGATG 4 cut(s) 123, 262, 505, 514
BtsIMutI CAGTG 2 cut(s) 652, 1107
Cac8I GCNNGC 6 cut(s) 264, 933, 1042, 1218, 1269, 1295
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 5 cut(s) 100, 219, 351, 463, 523
Cfr42I CCGCGG 1 cut(s) 267
CseI GACGC 1 cut(s) 63
Csp6I GTAC 2 cut(s) 822, 967
CspAI ACCGGT 1 cut(s) 240
CviAII CATG 7 cut(s) 250, 819, 972, 1032, 1041, 1165, 1222
CviQI GTAC 2 cut(s) 822, 967
DdeI CTNAG 4 cut(s) 441, 717, 838, 1002
DpnI GATC 3 cut(s) 364, 549, 577
DpnII GATC 3 cut(s) 362, 547, 575
DraIII CACNNNGTG 1 cut(s) 1168
EaeI YGGCCR 3 cut(s) 657, 780, 1281
EagI CGGCCG 1 cut(s) 657
Eam1104I CTCTTC 4 cut(s) 402, 611, 622, 1194
EarI CTCTTC 4 cut(s) 402, 611, 622, 1194
EciI GGCGGA 2 cut(s) 284, 295
EclXI CGGCCG 1 cut(s) 657
Eco130I CCWWGG 2 cut(s) 959, 1221
Eco24I GRGCYC 2 cut(s) 264, 317
Eco47I GGWCC 5 cut(s) 100, 219, 351, 463, 523
Eco52I CGGCCG 1 cut(s) 657
Eco57I CTGAAG 2 cut(s) 628, 1083
EcoNI CCTNNNNNAGG 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 706
EcoRII CCWGG 2 cut(s) 335, 353
EcoT14I CCWWGG 2 cut(s) 959, 1221
EcoT38I GRGCYC 2 cut(s) 264, 317
ErhI CCWWGG 2 cut(s) 959, 1221
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 7 cut(s) 253, 822, 975, 1035, 1044, 1168, 1225
FaqI GGGAC 2 cut(s) 86, 449
FatI CATG 7 cut(s) 249, 818, 971, 1031, 1040, 1164, 1221
FauI CCCGC 2 cut(s) 236, 271
FokI GGATG 4 cut(s) 110, 269, 512, 521
FriOI GRGCYC 2 cut(s) 264, 317
FspBI CTAG 3 cut(s) 671, 884, 1136
HaeIII GGCC 4 cut(s) 659, 782, 1220, 1283
HapII CCGG 3 cut(s) 241, 254, 1262
HgaI GACGC 1 cut(s) 63
Hin1II CATG 7 cut(s) 253, 822, 975, 1035, 1044, 1168, 1225
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 5 cut(s) 5, 376, 636, 856, 1090
HpaII CCGG 3 cut(s) 241, 254, 1262
HphI GGTGA 2 cut(s) 9, 1111
Hpy166II GTNNAC 1 cut(s) 640
Hpy188I TCNGA 5 cut(s) 49, 94, 367, 552, 720
Hpy188III TCNNGA 6 cut(s) 60, 380, 427, 521, 703, 840
Hpy8I GTNNAC 1 cut(s) 640
HpyAV CCTTC 9 cut(s) 91, 144, 210, 210, 342, 429, 439, 486, 1235
HpyCH4V TGCA 6 cut(s) 288, 494, 800, 812, 1171, 1190
HpyF3I CTNAG 4 cut(s) 441, 717, 838, 1002
Hsp92II CATG 7 cut(s) 253, 822, 975, 1035, 1044, 1168, 1225
KspI CCGCGG 1 cut(s) 267
Kzo9I GATC 3 cut(s) 362, 547, 575
LmnI GCTCC 7 cut(s) 50, 478, 887, 1037, 1207, 1252, 1264
LweI GCATC 2 cut(s) 1076, 1177
MaeI CTAG 3 cut(s) 671, 884, 1136
MaeIII GTNAC 2 cut(s) 75, 1148
MalI GATC 3 cut(s) 364, 549, 577
MboI GATC 3 cut(s) 362, 547, 575
MhlI GDGCHC 4 cut(s) 264, 317, 475, 602
MlsI TGGCCA 1 cut(s) 782
MluCI AATT 4 cut(s) 528, 706, 711, 1140
MluNI TGGCCA 1 cut(s) 782
MlyI GAGTC 3 cut(s) 385, 645, 850
MmeI TCCRAC 1 cut(s) 1005
Mox20I TGGCCA 1 cut(s) 782
MroXI GAANNNNTTC 1 cut(s) 833
MscI TGGCCA 1 cut(s) 782
MslI CAYNNNNRTG 3 cut(s) 645, 986, 1226
Msp20I TGGCCA 1 cut(s) 782
MspA1I CMGCKG 2 cut(s) 266, 538
MspI CCGG 3 cut(s) 241, 254, 1262
MspR9I CCNGG 2 cut(s) 337, 355
Mva1269I GAATGC 1 cut(s) 1124
MvaI CCWGG 2 cut(s) 337, 355
MvnI CGCG 2 cut(s) 74, 266
NcoI CCATGG 1 cut(s) 1221
NdeII GATC 3 cut(s) 362, 547, 575
NlaIII CATG 7 cut(s) 253, 822, 975, 1035, 1044, 1168, 1225
NlaIV GGNNCC 3 cut(s) 102, 273, 465
NmeAIII GCCGAG 1 cut(s) 635
NmuCI GTSAC 1 cut(s) 75
NspI RCATGY 3 cut(s) 253, 822, 1044
OliI CACNNNNGTG 1 cut(s) 645
PaeI GCATGC 1 cut(s) 1044
PciI ACATGT 1 cut(s) 818
PcsI WCGNNNNNNNCGW 1 cut(s) 1153
PctI GAATGC 1 cut(s) 1124
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 1 cut(s) 1090
PfoI TCCNGGA 1 cut(s) 353
PinAI ACCGGT 1 cut(s) 240
PleI GAGTC 3 cut(s) 384, 644, 850
PpsI GAGTC 3 cut(s) 384, 644, 850
PpuMI RGGWCCY 1 cut(s) 100
PscI ACATGT 1 cut(s) 818
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 335, 353
PspGI CCWGG 2 cut(s) 335, 353
PspN4I GGNNCC 3 cut(s) 102, 273, 465
PspPI GGNCC 5 cut(s) 100, 219, 351, 463, 523
PspPPI RGGWCCY 1 cut(s) 100
PstI CTGCAG 1 cut(s) 802
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 2 cut(s) 823, 968
RsaNI GTAC 2 cut(s) 822, 967
RseI CAYNNNNRTG 3 cut(s) 645, 986, 1226
SacII CCGCGG 1 cut(s) 267
Sau3AI GATC 3 cut(s) 362, 547, 575
Sau96I GGNCC 5 cut(s) 100, 219, 351, 463, 523
SchI GAGTC 3 cut(s) 385, 645, 850
ScrFI CCNGG 2 cut(s) 337, 355
SduI GDGCHC 4 cut(s) 264, 317, 475, 602
SfaNI GCATC 2 cut(s) 1076, 1177
SfcI CTRYAG 1 cut(s) 798
Sfr303I CCGCGG 1 cut(s) 267
SgrBI CCGCGG 1 cut(s) 267
SinI GGWCC 5 cut(s) 100, 219, 351, 463, 523
SmiMI CAYNNNNRTG 3 cut(s) 645, 986, 1226
SmlI CTYRAG 2 cut(s) 755, 860
SmoI CTYRAG 2 cut(s) 755, 860
SphI GCATGC 1 cut(s) 1044
Sse9I AATT 4 cut(s) 528, 706, 711, 1140
SspMI CTAG 3 cut(s) 671, 884, 1136
StyD4I CCNGG 2 cut(s) 335, 353
StyI CCWWGG 2 cut(s) 959, 1221
TaqI TCGA 4 cut(s) 246, 379, 704, 732
TaqII GACCGA 2 cut(s) 451, 540
TasI AATT 4 cut(s) 528, 706, 711, 1140
TatI WGTACW 2 cut(s) 821, 966
TauI GCSGC 2 cut(s) 269, 1261
TfiI GAWTC 1 cut(s) 1090
TscAI CASTG 2 cut(s) 652, 1107
TseFI GTSAC 1 cut(s) 75
Tsp45I GTSAC 1 cut(s) 75
TspDTI ATGAA 4 cut(s) 477, 684, 1004, 1117
TspGWI ACGGA 1 cut(s) 132
TspRI CASTG 2 cut(s) 652, 1107
VpaK11BI GGWCC 5 cut(s) 100, 219, 351, 463, 523
XagI CCTNNNNNAGG 1 cut(s) 334
XapI RAATTY 1 cut(s) 706
XceI RCATGY 3 cut(s) 253, 822, 1044
XmnI GAANNNNTTC 1 cut(s) 833
XspI CTAG 3 cut(s) 671, 884, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.