AT2G21720

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012209
Physical Location & Seq
Forward (+)
9273696 .. 9276961
3266 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21720.2

Sequence Viewer

Length: 2205 bp
ATGATTCTCAGTTTTCGGCTATTTGCATGGGAGTGCCACGTGGCATGTGGAGAAGTTGCCAGTGAACGAGTTATTCTCTCTACCTCAACGTCACTAAAACCGAATGCGAAAAGGAAAAAAAATAGCAAAAGCAAAAAACTAAAACAGAGGATTCTCAGAGATCACGAGAGCAAGAAAACGATGAGGAAGATGAAGCACTTGTCCTCCATTGCAAACGATGTCGTTCAGAGATGCTCACAGGAACTAGAGACAACCATTGATGATTTAGTGAAAGAATTTGAATGTCAATGGAAGCCAGGAAGCACGGGAGGTACTTACTCGAAGAAGTTTGTGGAATTTTGCAACTCAAAGGTGACGAGTCGGGTTTGTGAAAACATACTTGAGAGAATAAAAGATGGTTCCTTTACACGTCTCACGTTCGATATGATGCTTGCGTGGCAACAACCTGACGCAGATGATAATGAGTCTTATAAGGAAGCAGTAGGTAAAGAGAGTGAAGACAAGAGGATTCAAGCAACTTTATCCCCAGAGCAAGATGACATCTCGCTTTTTTATTCAGACATGATGCCGCTTCTCGTTGATCATGAACCAAGTGTGGGTGAAGATGCTTTTGTCTATTTAGGCTCAATCATTCCTCTTCCCGTGGATATCATCAACGGAAGATACACATTTGAGACTCTAACTGCTCCGACCGGTCACCAACTTCACTTCCCAGCTTATGATATGTTTGTCAAAGAAATACACAAGTGTATGAAGCATTTGCAAAAGCAATCGACGCCTAAAGGGATCGAATTAGCGGATGATGAAATTATATTGCACGTTGAAGGAACAATGGCTTCACAGAGAGTGATTCGCCACATTAAAGAAACAAGCTGGCCAGGTAGGCTTACTTTGACAAACTATGCACTATACTTCGAAGCCGCGGGAATTATAAACTATGAAGATGCTATAAAGATAGATCTTTCTAAGGACAATGAAAAGTCTACAAAGCCTATGTCCACTGGTCCATTGGGTGCTCCTCTTTTTGACAAGGCCATTGTCTACGAGTCTCCAGATTTTGAGGAGGGAATAGTCATAGAGTTTCCGGAGATGACAAGCTCAACGAGACGAGATCATTGGCTTATGCTTGTAAAAGAAATAACATTAATGCACAAGTTCTTGAGGAAATTCAATGTTGAGTCTCCCTTGCAATCATGGGAAATCCATTCAAGAACAATCTTGGGAATAATACGGTTGCACGCAGCCCGCGAGATGTTAAGAATTTCTCCCCCGGATCCAAAAAACTTTTTAATATTTAGTTTGTTTGAGGAAGTTCCAAAGGGAGACTATGTACTTGAGGAGCTCGCAGAGATTAGTCTAAAGATAGGCACTACCAGAAACCCGTGTAGTGCTAGCTCCATATTGAGGAATATGAACATGGATCAGCTTGGTGACATGATAAAAGAAGAGGGTGAAGATATATGCAAGGAGAAGGTGGTCAAAGTGACTGATAAAGAAGAAATGCTTGCGTCTCTTGAGAGTGCAGTGAATCAATCTAGAGAAGAGGGAAAGGTTATTGAAAAGGCTAGAGCCACGACAGCGGAACTCGAAGAAGAAGGGATTAGTGAAAGTGTAGCAGTTCTTATGGAGTTGTTGAGGCCGTTGCAAGACGTATTACCATGGTTCCAAGAAGTGATTTATTGGGAAAGGCCGTCTCGTACACTGTTTGTTTTAGCCATTACTATTTTAACCGTCTACAAGGAGTGGGTTGGTAAGGCTATAGCTGCTTGCTTAATATGGGTGGTGGCAAAAATGGCTCAAGCTAGAAATAAGATGGTCCATACAAAAAGTGAGGATGCGGTAACAGTGAGCACTGAATCTGACCAAACAGTGACTGAGAGCATTGTGTCTGCTCAATATGGCTTAATTAGACTTCACCAGTTGATGCAACATGTTAACGTCACCATCTTGAAATTGCGGTCTCTATATACCTCCAAGGCTAGCAAGCATGCAAGCATGGTGATGGCGTTGATGCTAGTGTTGGCGAGCTTCTTCGCAGTGGTGCCGTTCAAGTTGTTCATAATATTTGGTATAGTATATTGCTTTGTAATGACATCAAGCGTCGGAACATACATGAGCAACGATCAGAGTAACCGGAGAATGAAAGAGTGGTGGGATTCCATACCCATTGTTCCCGTTCGTGTACGTAACGCTTCGTCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

734

Amino Acids

83.6

Weight (kDa)

6.2

Isoelectric Point (pI)

48.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 505 - 735 4.8e-112 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21720 AT2G21720
fragaria_vesca FvH4_1g28470 FvH4_1g28470 FvH4_1g28470
malus_domestica MD12G1008600.v1.1 MD14G1006000.v1.1
prunus_persica Prupe.7G010400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0496361 RchiOBHm_Chr3g0496371
rosa_laevigata RLG00000022528
rosa_multiflora Rmu_sc0002461.1_g000006
rosa_roxburghii Rroxscaffold_6G00389250
rosa_rugosa Rorug03G0282000
rosa_samantha Rh3AG328600 Rh3BG364700 Rh3CG361700 Rh3DG364700
rosa_wichuraiana Rw3G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 471, 932
AccB1I GGYRCC 1 cut(s) 2041
AccI GTMKAC 3 cut(s) 983, 1041, 1734
AccII CGCG 2 cut(s) 923, 1248
AccIII TCCGGA 1 cut(s) 1084
AciI CCGC 8 cut(s) 569, 797, 921, 923, 1246, 1580, 1838, 1957
AclWI GGATC 4 cut(s) 794, 1268, 1281, 1428
AcoI YGGCCR 1 cut(s) 875
AcsI RAATTY 4 cut(s) 275, 335, 1166, 1260
AcvI CACGTG 1 cut(s) 40
AcyI GRCGYC 1 cut(s) 776
AfaI GTAC 4 cut(s) 313, 1332, 1699, 2184
AfiI CCNNNNNNNGG 2 cut(s) 596, 1404
AflIII ACRYGT 2 cut(s) 407, 1930
AgeI ACCGGT 1 cut(s) 692
AgsI TTSAA 8 cut(s) 281, 512, 824, 1171, 1209, 1559, 1951, 2050
AjiI CACGTC 1 cut(s) 410
AjnI CCWGG 2 cut(s) 295, 877
AluBI AGCT 9 cut(s) 716, 873, 1098, 1342, 1395, 1426, 1763, 1802, 2028
AluI AGCT 9 cut(s) 716, 873, 1098, 1342, 1395, 1426, 1763, 1802, 2028
Alw21I GWGCWC 3 cut(s) 1018, 1344, 1853
AlwI GGATC 4 cut(s) 794, 1268, 1281, 1428
AlwNI CAGNNNCTG 1 cut(s) 1874
Aor13HI TCCGGA 1 cut(s) 1084
AoxI GGCC 4 cut(s) 875, 1032, 1637, 1688
ApeKI GCWGC 2 cut(s) 1241, 1763
ApoI RAATTY 4 cut(s) 275, 335, 1166, 1260
ArsI GACNNNNNNTTYG 2 cut(s) 980, 1012
AseI ATTAAT 1 cut(s) 1145
AsiGI ACCGGT 1 cut(s) 692
AspS9I GGNCC 2 cut(s) 1004, 1816
AsuC2I CCSGG 1 cut(s) 1271
AsuHPI GGTGA 8 cut(s) 364, 611, 689, 1442, 1463, 1907, 1933, 2011
AsuII TTCGAA 1 cut(s) 915
AsuNHI GCTAGC 2 cut(s) 1391, 1979
AvaII GGWCC 2 cut(s) 1004, 1816
BalI TGGCCA 1 cut(s) 877
BamHI GGATCC 1 cut(s) 1273
BanI GGYRCC 1 cut(s) 2041
BanII GRGCYC 1 cut(s) 1344
BauI CACGAG 1 cut(s) 164
BbrPI CACGTG 1 cut(s) 40
BbsI GAAGAC 1 cut(s) 504
Bbv12I GWGCWC 3 cut(s) 1018, 1344, 1853
BbvI GCAGC 2 cut(s) 1253, 1750
BccI CCATC 4 cut(s) 389, 1807, 1952, 1996
BceAI ACGGC 3 cut(s) 1624, 1675, 2029
BciT130I CCWGG 2 cut(s) 297, 879
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 1 cut(s) 1271
BfaI CTAG 7 cut(s) 245, 1392, 1536, 1566, 1803, 1980, 2015
BfmI CTRYAG 1 cut(s) 1758
BglI GCCNNNNNGGC 1 cut(s) 883
BglII AGATCT 1 cut(s) 958
BisI GCNGC 4 cut(s) 569, 921, 1242, 1764
BlsI GCNGC 4 cut(s) 570, 922, 1243, 1765
Bme1390I CCNGG 3 cut(s) 297, 879, 1271
Bme18I GGWCC 2 cut(s) 1004, 1816
BmgBI CACGTC 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 1004, 1816
BmiI GGNNCC 4 cut(s) 400, 1275, 1664, 2043
BmrFI CCNGG 3 cut(s) 297, 879, 1271
BmsI GCATC 8 cut(s) 221, 417, 555, 595, 934, 1825, 1914, 2001
BmtI GCTAGC 2 cut(s) 1395, 1983
BpiI GAAGAC 1 cut(s) 504
BplI GAGNNNNNCTC 2 cut(s) 60, 92
BpmI CTGGAG 1 cut(s) 1035
Bpu14I TTCGAA 1 cut(s) 915
BpuEI CTTGAG 5 cut(s) 401, 1180, 1355, 1535, 1782
BpuMI CCSGG 1 cut(s) 1271
BsaAI YACGTR 2 cut(s) 40, 2186
BsaHI GRCGYC 1 cut(s) 776
BsaI GGTCTC 1 cut(s) 1965
BsaJI CCNNGG 5 cut(s) 642, 921, 1269, 1658, 1974
BsaWI WCCGGW 3 cut(s) 692, 1084, 2133
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 1314, 1344
Bsc4I CCNNNNNNNGG 2 cut(s) 596, 1404
Bse118I RCCGGY 1 cut(s) 692
Bse1I ACTGG 3 cut(s) 60, 1006, 1918
Bse3DI GCAATG 1 cut(s) 207
BseAI TCCGGA 1 cut(s) 1084
BseBI CCWGG 2 cut(s) 297, 879
BseDI CCNNGG 5 cut(s) 642, 921, 1269, 1658, 1974
BseGI GGATG 2 cut(s) 805, 1840
BseLI CCNNNNNNNGG 2 cut(s) 596, 1404
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 3 cut(s) 22, 169, 1866
BseNI ACTGG 3 cut(s) 60, 1006, 1918
BseRI GAGGAG 3 cut(s) 1008, 1076, 1352
BseXI GCAGC 2 cut(s) 1253, 1750
BseYI CCCAGC 1 cut(s) 712
BsgI GTGCAG 1 cut(s) 1542
Bsh1236I CGCG 2 cut(s) 923, 1248
Bsh1285I CGRYCG 1 cut(s) 693
BshFI GGCC 4 cut(s) 877, 1034, 1639, 1690
BshNI GGYRCC 1 cut(s) 2041
BshTI ACCGGT 1 cut(s) 692
BsiEI CGRYCG 1 cut(s) 693
BsiHKAI GWGCWC 3 cut(s) 1018, 1344, 1853
BsiSI CCGG 4 cut(s) 693, 1085, 1271, 2134
BslI CCNNNNNNNGG 2 cut(s) 596, 1404
BsmBI CGTCTC 4 cut(s) 416, 1099, 1515, 1698
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 4 cut(s) 877, 1034, 1639, 1690
Bso31I GGTCTC 1 cut(s) 1965
Bsp119I TTCGAA 1 cut(s) 915
Bsp1286I GDGCHC 3 cut(s) 1018, 1344, 1853
Bsp13I TCCGGA 1 cut(s) 1084
Bsp143I GATC 8 cut(s) 160, 580, 786, 958, 1111, 1273, 1420, 2122
Bsp19I CCATGG 1 cut(s) 1658
BspACI CCGC 8 cut(s) 569, 797, 921, 923, 1246, 1580, 1838, 1957
BspANI GGCC 4 cut(s) 877, 1034, 1639, 1690
BspCNI CTCAG 3 cut(s) 21, 168, 1867
BspEI TCCGGA 1 cut(s) 1084
BspFNI CGCG 2 cut(s) 923, 1248
BspHI TCATGA 1 cut(s) 583
BspLI GGNNCC 4 cut(s) 400, 1275, 1664, 2043
BspOI GCTAGC 2 cut(s) 1395, 1983
BspPI GGATC 4 cut(s) 794, 1268, 1281, 1428
BspT104I TTCGAA 1 cut(s) 915
BspT107I GGYRCC 1 cut(s) 2041
BspTNI GGTCTC 1 cut(s) 1965
BsrDI GCAATG 1 cut(s) 207
BsrFI RCCGGY 1 cut(s) 692
BsrI ACTGG 3 cut(s) 60, 1006, 1918
BssAI RCCGGY 1 cut(s) 692
BssECI CCNNGG 5 cut(s) 642, 921, 1269, 1658, 1974
BssMI GATC 8 cut(s) 160, 580, 786, 958, 1111, 1273, 1420, 2122
BssNI GRCGYC 1 cut(s) 776
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 2 cut(s) 1658, 1974
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 2 cut(s) 297, 879
Bst4CI ACNGT 5 cut(s) 1233, 1704, 1732, 1846, 1870
Bst6I CTCTTC 3 cut(s) 642, 1440, 1536
BstACI GRCGYC 1 cut(s) 776
BstBAI YACGTR 2 cut(s) 40, 2186
BstBI TTCGAA 1 cut(s) 915
BstDEI CTNAG 4 cut(s) 8, 155, 966, 1875
BstDSI CCRYGG 3 cut(s) 642, 921, 1658
BstEII GGTNACC 1 cut(s) 695
BstF5I GGATG 2 cut(s) 805, 1840
BstFNI CGCG 2 cut(s) 923, 1248
BstKTI GATC 8 cut(s) 163, 583, 789, 961, 1114, 1276, 1423, 2125
BstMBI GATC 8 cut(s) 160, 580, 786, 958, 1111, 1273, 1420, 2122
BstMCI CGRYCG 1 cut(s) 693
BstMWI GCNNNNNNNGC 6 cut(s) 436, 775, 883, 1577, 1763, 1793
BstNI CCWGG 2 cut(s) 297, 879
BstNSI RCATGY 3 cut(s) 48, 1934, 1991
BstPI GGTNACC 1 cut(s) 695
BstSCI CCNGG 3 cut(s) 295, 877, 1269
BstSFI CTRYAG 1 cut(s) 1758
BstSNI TACGTA 1 cut(s) 2186
BstUI CGCG 2 cut(s) 923, 1248
BstV1I GCAGC 2 cut(s) 1253, 1750
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 2 cut(s) 958, 1273
BstYI RGATCY 2 cut(s) 958, 1273
BsuRI GGCC 4 cut(s) 877, 1034, 1639, 1690
BtgI CCRYGG 3 cut(s) 642, 921, 1658
BtrI CACGTC 1 cut(s) 410
BtsCI GGATG 2 cut(s) 805, 1840
BtsI GCAGTG 2 cut(s) 1530, 2043
BtsIMutI CAGTG 8 cut(s) 67, 999, 1530, 1700, 1851, 1851, 1875, 2043
CaiI CAGNNNCTG 1 cut(s) 1874
CciI TCATGA 1 cut(s) 583
Cfr10I RCCGGY 1 cut(s) 692
Cfr13I GGNCC 2 cut(s) 1004, 1816
Cfr42I CCGCGG 1 cut(s) 924
CseI GACGC 4 cut(s) 458, 784, 1497, 2089
Csp6I GTAC 4 cut(s) 312, 1331, 1698, 2183
CspAI ACCGGT 1 cut(s) 692
CviQI GTAC 4 cut(s) 312, 1331, 1698, 2183
DdeI CTNAG 4 cut(s) 8, 155, 966, 1875
DpnI GATC 8 cut(s) 162, 582, 788, 960, 1113, 1275, 1422, 2124
DpnII GATC 8 cut(s) 160, 580, 786, 958, 1111, 1273, 1420, 2122
EaeI YGGCCR 1 cut(s) 875
Eam1104I CTCTTC 3 cut(s) 642, 1440, 1536
EarI CTCTTC 3 cut(s) 642, 1440, 1536
Ecl136II GAGCTC 1 cut(s) 1342
Eco105I TACGTA 1 cut(s) 2186
Eco130I CCWWGG 2 cut(s) 1658, 1974
Eco24I GRGCYC 1 cut(s) 1344
Eco31I GGTCTC 1 cut(s) 1965
Eco32I GATATC 1 cut(s) 649
Eco47I GGWCC 2 cut(s) 1004, 1816
Eco53kI GAGCTC 1 cut(s) 1342
Eco72I CACGTG 1 cut(s) 40
Eco91I GGTNACC 1 cut(s) 695
EcoICRI GAGCTC 1 cut(s) 1342
EcoO65I GGTNACC 1 cut(s) 695
EcoRII CCWGG 2 cut(s) 295, 877
EcoRV GATATC 1 cut(s) 649
EcoT14I CCWWGG 2 cut(s) 1658, 1974
EcoT38I GRGCYC 1 cut(s) 1344
ErhI CCWWGG 2 cut(s) 1658, 1974
Esp3I CGTCTC 4 cut(s) 416, 1099, 1515, 1698
FalI AAGNNNNNCTT 2 cut(s) 1488, 1520
FauI CCCGC 2 cut(s) 916, 1253
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 3 cut(s) 983, 1041, 1734
Fnu4HI GCNGC 4 cut(s) 569, 921, 1242, 1764
FokI GGATG 2 cut(s) 812, 1847
FriOI GRGCYC 1 cut(s) 1344
Fsp4HI GCNGC 4 cut(s) 569, 921, 1242, 1764
FspBI CTAG 7 cut(s) 245, 1392, 1536, 1566, 1803, 1980, 2015
GluI GCNGC 4 cut(s) 569, 921, 1242, 1764
GsaI CCCAGC 1 cut(s) 716
GsuI CTGGAG 1 cut(s) 1035
HaeIII GGCC 4 cut(s) 877, 1034, 1639, 1690
HapII CCGG 4 cut(s) 693, 1085, 1271, 2134
HgaI GACGC 4 cut(s) 458, 784, 1497, 2089
Hin1I GRCGYC 1 cut(s) 776
HincII GTYRAC 1 cut(s) 1936
HindII GTYRAC 1 cut(s) 1936
HpaI GTTAAC 1 cut(s) 1936
HpaII CCGG 4 cut(s) 693, 1085, 1271, 2134
HphI GGTGA 8 cut(s) 364, 611, 689, 1442, 1463, 1907, 1933, 2011
Hpy166II GTNNAC 8 cut(s) 65, 984, 999, 1042, 1700, 1735, 1936, 2183
Hpy188I TCNGA 7 cut(s) 158, 228, 559, 690, 1861, 2105, 2127
Hpy188III TCNNGA 9 cut(s) 164, 584, 1052, 1085, 1159, 1209, 1514, 1536, 1948
Hpy8I GTNNAC 8 cut(s) 65, 984, 999, 1042, 1700, 1735, 1936, 2183
Hpy99I CGWCG 2 cut(s) 778, 2105
HpyAV CCTTC 3 cut(s) 818, 1465, 1589
HpyCH4III ACNGT 5 cut(s) 1233, 1704, 1732, 1846, 1870
HpyCH4IV ACGT 8 cut(s) 39, 89, 409, 416, 819, 1650, 1938, 2185
HpyF10VI GCNNNNNNNGC 6 cut(s) 436, 775, 883, 1577, 1763, 1793
HpyF3I CTNAG 4 cut(s) 8, 155, 966, 1875
HpySE526I ACGT 8 cut(s) 39, 89, 409, 416, 819, 1650, 1938, 2185
Hsp92I GRCGYC 1 cut(s) 776
Kpn2I TCCGGA 1 cut(s) 1084
Ksp22I TGATCA 1 cut(s) 580
KspAI GTTAAC 1 cut(s) 1936
KspI CCGCGG 1 cut(s) 924
Kzo9I GATC 8 cut(s) 160, 580, 786, 958, 1111, 1273, 1420, 2122
LmnI GCTCC 4 cut(s) 691, 1021, 1339, 1400
Lsp1109I GCAGC 2 cut(s) 1253, 1750
LweI GCATC 8 cut(s) 221, 417, 555, 595, 934, 1825, 1914, 2001
MaeI CTAG 7 cut(s) 245, 1392, 1536, 1566, 1803, 1980, 2015
MaeII ACGT 8 cut(s) 39, 89, 409, 416, 819, 1650, 1938, 2185
MalI GATC 8 cut(s) 162, 582, 788, 960, 1113, 1275, 1422, 2124
MboI GATC 8 cut(s) 160, 580, 786, 958, 1111, 1273, 1420, 2122
MflI RGATCY 2 cut(s) 958, 1273
MhlI GDGCHC 3 cut(s) 1018, 1344, 1853
MlsI TGGCCA 1 cut(s) 877
MluCI AATT 9 cut(s) 275, 335, 791, 807, 927, 1166, 1260, 1905, 1952
MluNI TGGCCA 1 cut(s) 877
MlyI GAGTC 5 cut(s) 367, 473, 670, 1055, 1187
MmeI TCCRAC 2 cut(s) 713, 2083
Mox20I TGGCCA 1 cut(s) 877
MroI TCCGGA 1 cut(s) 1084
MscI TGGCCA 1 cut(s) 877
MseI TTAA 8 cut(s) 861, 1145, 1256, 1289, 1727, 1772, 1904, 1935
MslI CAYNNNNRTG 2 cut(s) 31, 2000
Msp20I TGGCCA 1 cut(s) 877
MspA1I CMGCKG 2 cut(s) 923, 1580
MspI CCGG 4 cut(s) 693, 1085, 1271, 2134
MspR9I CCNGG 3 cut(s) 297, 879, 1271
Mva1269I GAATGC 1 cut(s) 109
MvaI CCWGG 2 cut(s) 297, 879
MvnI CGCG 2 cut(s) 923, 1248
MwoI GCNNNNNNNGC 6 cut(s) 436, 775, 883, 1577, 1763, 1793
NciI CCSGG 1 cut(s) 1271
NcoI CCATGG 1 cut(s) 1658
NdeII GATC 8 cut(s) 160, 580, 786, 958, 1111, 1273, 1420, 2122
NheI GCTAGC 2 cut(s) 1391, 1979
NlaIV GGNNCC 4 cut(s) 400, 1275, 1664, 2043
NmuCI GTSAC 7 cut(s) 90, 352, 695, 1430, 1483, 1870, 1939
NspI RCATGY 3 cut(s) 48, 1934, 1991
NspV TTCGAA 1 cut(s) 915
PaeI GCATGC 1 cut(s) 1991
PagI TCATGA 1 cut(s) 583
PciI ACATGT 1 cut(s) 1930
PcsI WCGNNNNNNNCGW 1 cut(s) 1245
PctI GAATGC 1 cut(s) 109
PfeI GAWTC 7 cut(s) 4, 151, 508, 850, 1528, 1856, 2156
PinAI ACCGGT 1 cut(s) 692
PkrI GCNGC 4 cut(s) 570, 922, 1243, 1765
PleI GAGTC 5 cut(s) 366, 472, 670, 1054, 1186
PmaCI CACGTG 1 cut(s) 40
PmlI CACGTG 1 cut(s) 40
PpsI GAGTC 5 cut(s) 366, 472, 670, 1054, 1186
Ppu21I YACGTR 2 cut(s) 40, 2186
PscI ACATGT 1 cut(s) 1930
PshBI ATTAAT 1 cut(s) 1145
PsiI TTATAA 2 cut(s) 471, 932
Psp124BI GAGCTC 1 cut(s) 1344
Psp6I CCWGG 2 cut(s) 295, 877
PspCI CACGTG 1 cut(s) 40
PspEI GGTNACC 1 cut(s) 695
PspFI CCCAGC 1 cut(s) 712
PspGI CCWGG 2 cut(s) 295, 877
PspN4I GGNNCC 4 cut(s) 400, 1275, 1664, 2043
PspPI GGNCC 2 cut(s) 1004, 1816
PstNI CAGNNNCTG 1 cut(s) 1874
PsuI RGATCY 2 cut(s) 958, 1273
RsaI GTAC 4 cut(s) 313, 1332, 1699, 2184
RsaNI GTAC 4 cut(s) 312, 1331, 1698, 2183
RseI CAYNNNNRTG 2 cut(s) 31, 2000
SacI GAGCTC 1 cut(s) 1344
SacII CCGCGG 1 cut(s) 924
SaqAI TTAA 8 cut(s) 861, 1145, 1256, 1289, 1727, 1772, 1904, 1935
SatI GCNGC 4 cut(s) 569, 921, 1242, 1764
Sau3AI GATC 8 cut(s) 160, 580, 786, 958, 1111, 1273, 1420, 2122
Sau96I GGNCC 2 cut(s) 1004, 1816
SchI GAGTC 5 cut(s) 367, 473, 670, 1055, 1187
ScrFI CCNGG 3 cut(s) 297, 879, 1271
SduI GDGCHC 3 cut(s) 1018, 1344, 1853
SfaNI GCATC 8 cut(s) 221, 417, 555, 595, 934, 1825, 1914, 2001
SfcI CTRYAG 1 cut(s) 1758
Sfr303I CCGCGG 1 cut(s) 924
SfuI TTCGAA 1 cut(s) 915
SgrBI CCGCGG 1 cut(s) 924
SinI GGWCC 2 cut(s) 1004, 1816
SmiMI CAYNNNNRTG 2 cut(s) 31, 2000
SmlI CTYRAG 5 cut(s) 380, 1159, 1334, 1514, 1797
SmoI CTYRAG 5 cut(s) 380, 1159, 1334, 1514, 1797
SnaBI TACGTA 1 cut(s) 2186
SphI GCATGC 1 cut(s) 1991
Sse9I AATT 9 cut(s) 275, 335, 791, 807, 927, 1166, 1260, 1905, 1952
SsiI CCGC 8 cut(s) 569, 797, 921, 923, 1246, 1580, 1838, 1957
SspI AATATT 2 cut(s) 1293, 2064
SspMI CTAG 7 cut(s) 245, 1392, 1536, 1566, 1803, 1980, 2015
SstI GAGCTC 1 cut(s) 1344
StyD4I CCNGG 3 cut(s) 295, 877, 1269
StyI CCWWGG 2 cut(s) 1658, 1974
TaaI ACNGT 5 cut(s) 1233, 1704, 1732, 1846, 1870
TaiI ACGT 8 cut(s) 42, 92, 412, 419, 822, 1653, 1941, 2188
TaqI TCGA 6 cut(s) 320, 420, 773, 789, 915, 1587
TasI AATT 9 cut(s) 275, 335, 791, 807, 927, 1166, 1260, 1905, 1952
TatI WGTACW 1 cut(s) 1330
TauI GCSGC 2 cut(s) 571, 923
TfiI GAWTC 7 cut(s) 4, 151, 508, 850, 1528, 1856, 2156
Tru1I TTAA 8 cut(s) 861, 1145, 1256, 1289, 1727, 1772, 1904, 1935
Tru9I TTAA 8 cut(s) 861, 1145, 1256, 1289, 1727, 1772, 1904, 1935
TscAI CASTG 8 cut(s) 67, 1006, 1530, 1707, 1851, 1858, 1875, 2043
TseFI GTSAC 7 cut(s) 90, 352, 695, 1430, 1483, 1870, 1939
TseI GCWGC 2 cut(s) 1241, 1763
Tsp45I GTSAC 7 cut(s) 90, 352, 695, 1430, 1483, 1870, 1939
TspDTI ATGAA 9 cut(s) 206, 600, 767, 819, 954, 990, 1427, 2047, 2156
TspGWI ACGGA 1 cut(s) 672
TspRI CASTG 8 cut(s) 67, 1006, 1530, 1707, 1851, 1858, 1875, 2043
VpaK11BI GGWCC 2 cut(s) 1004, 1816
VspI ATTAAT 1 cut(s) 1145
XapI RAATTY 4 cut(s) 275, 335, 1166, 1260
XbaI TCTAGA 1 cut(s) 1535
XceI RCATGY 3 cut(s) 48, 1934, 1991
XcmI CCANNNNNNNNNTGG 2 cut(s) 44, 1006
XmiI GTMKAC 3 cut(s) 983, 1041, 1734
XspI CTAG 7 cut(s) 245, 1392, 1536, 1566, 1803, 1980, 2015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.