MD14G1006000.v1.1

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012209
Physical Location & Seq
Forward (+)
642710 .. 646756
4047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1006000.v1.1.491

Sequence Viewer

Length: 2187 bp
ATGCAGAAACACATCTCAGCCTCTGCCTCTCCATCTCCTTCTCCTTCCTCCCTCCTCCTTCCTGCTTCCTCCCTATTGAAAACCAAACAATACTCTGGTCTAATTTCTCTGCAGATCTGCAAAAAACCAACAATGTCCACAATGAGGTTGAAGCATCTTTCCTCCATTGCAAACGATGTCGTACAAAAATGCGCCTTGAAACTGGAAACTCCGGTGGATAAATTGGTGGAGGAATTCGAAACTACATGGAAGACGGATGAAACGGTGCCGGTTGGTGCTAATAACAACAGTAATAGTTATGGGAGGAAATTGGTAGAATTTTGCAGTGCCAAGGCTCTGCAGAGTAGTGACATGTGCAAAACCATTGAGGAAAAGATTGCCAATGGATTTTTTAGCCGCTTCACTTTCGACATGATGCTCGCTTGGGAGATGCCTACCTCTGCCGACGAAGAATCTTTTGAGGAGTGCGTAGCGAAGGAGAAAGAAGAGAAAAAATTACCTGGAAAATTTCGTTCGGAGCAAGATGACGTCCCTTTGTTCTATTCAGACCTCATGCCACTTCTTGTTGATAACGAGAAAGATGTTGGAGAAGATGCATTTGTTTGGATGGGGTCGCTGGTTCCACTAGTTACAGATGTTGTCAATGGGAGATTTACCTTTGAAACTTTAACGGCGTCTACAGGAAACCGGCTCCATTTTCCATCCTACAACAAATATTTAAAGGAAATTGATAGATGTATAAAACATTTGGTAAAACATGCAAAACCGAAGGGTGTGGAGCTTGCAGACGACGAATTCATTTTACACGTCGAGGGAACTGCTAGCACACAGCGAGTAGTGCGCCACATTGGAGGAACAAGTTGGCCTGGTAGGCTTACACTAACAAATTACGCACTCTATTTCGAGGCTTCTGGAGTATTAACGTATGAAGATGCTCTTAAAATAGACCTTACAAGGGATGTGGAGCAAACGGTGAAGCCAGCTTCAACAGGGCCATGGGGTGCTCCACTTTTTGACAAGGCTATAATTTACGAGTCTCCTGAATTATCAGAGGGGATTATTCTGGAGTTCCCTGAGATAACAAGCTCTACAAGGCGGGACCATTGGCTTGCCCTCTCCAAGGAGATTATGTTGATGCATCGTTTCCTAGCAAAGTATGAGATAAACTGTCCGATACAAGCATGGGAGATGCATTCAAGGACAATCCTAGCAATAACAAGGCTCCATGCAGCAAGAGAAATGCTGAGAATATCACCCCCTCCACCAACAAGGTTCTTAATTTTTTCTTTGTTCGATGAGATACCGAAGGGAGATTATGTCCTGGAGGAGCTTGCGGAGAGTCTTAAGAAGGGAAATAGCGGGCATCCATGTAGTGCTAGTTCAATCCTAAGGAGCATGAACTTGTTAGAGTCAATGATCATCTCAAGTTTAGTTCAAGAGACAGAAGTAGGCAGCAGCGAAAGTGCAACCCCAACTCCAAGCGGTCAAGTCGAAGACAGTTCCTTACTAGAAACAGCAATTAATCAAGCAAGAGAGGAAGAAAAGGAAATTGCTATTGCCAAAGCTACCACAAAGGAACTTAAAGAGGAGGGAATCAGTGAAAGTGCTACTACCTTTTTGGCAATGCCTTGGTTTGAAGAAGTACTCGCATGGGAAAGACCGTCAACCACTCTCACTGTGGTTTCTGTGGCTTTAATAGTCACCTATCAGGAGTGGGTTGGCAAAGCAATAGCTGCTTTCTTCATTTCGCTGGTTGCAAAGATGGTTAGAGCAAGACGGCAAAGACTTGATATAAAATGCAATGAGATAGTCGTGTGCACCGCCTCGGACCAGAGTACAATAGAAAGCATAGTGTCTGCTCAACATGGAATGCAAACTGTGCATGAGATGGTGCAGATAGCAAATGTTTCGATATTGAAGTTGTGGTCCATATATATTTCGAAGGCTCGCAAGCATGCAAATGCGGCGATGGTGTTGTTGAGTGCCATCGCAATATTTTTGGCGGTGGTTCCGGTGAAGTTCATTATAATGGCAGCCATAGTTTGTTGCTTCTCTTCGACATTGGCATCAAGAATAGGTACGAGTAAGGGGGAGAACCAAAGCAACAGACGACTAAGAGAATGGTGGGATTCAATTCCAGTCATTCCTGTCCGTGTTGTCCAGACTCGAGAGTCCAAGACAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

729

Amino Acids

81.25

Weight (kDa)

5.82

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 502 - 722 3.8e-56 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21720 AT2G21720
fragaria_vesca FvH4_1g28470 FvH4_1g28470 FvH4_1g28470
malus_domestica MD12G1008600.v1.1 MD14G1006000.v1.1
prunus_persica Prupe.7G010400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0496361 RchiOBHm_Chr3g0496371
rosa_laevigata RLG00000022528
rosa_multiflora Rmu_sc0002461.1_g000006
rosa_roxburghii Rroxscaffold_6G00389250
rosa_rugosa Rorug03G0282000
rosa_samantha Rh3AG328600 Rh3BG364700 Rh3CG361700 Rh3DG364700
rosa_wichuraiana Rw3G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2027
AasI GACNNNNNNGTC 1 cut(s) 2170
AatII GACGTC 1 cut(s) 531
AccB1I GGYRCC 1 cut(s) 265
AccI GTMKAC 1 cut(s) 677
AciI CCGC 8 cut(s) 397, 1096, 1334, 1359, 1482, 1821, 1964, 2003
AcsI RAATTY 4 cut(s) 233, 317, 506, 794
AcyI GRCGYC 2 cut(s) 528, 674
AfaI GTAC 4 cut(s) 183, 1644, 1837, 2080
AfiI CCNNNNNNNGG 3 cut(s) 144, 955, 1120
AflII CTTAAG 1 cut(s) 1343
AflIII ACRYGT 2 cut(s) 351, 805
AhlI ACTAGT 1 cut(s) 625
AjiI CACGTC 1 cut(s) 808
AjnI CCWGG 3 cut(s) 499, 865, 1320
AluBI AGCT 6 cut(s) 781, 983, 1086, 1330, 1565, 1733
AluI AGCT 6 cut(s) 781, 983, 1086, 1330, 1565, 1733
Alw21I GWGCWC 2 cut(s) 1006, 1820
Alw26I GTCTC 2 cut(s) 1041, 1433
Alw44I GTGCAC 1 cut(s) 1816
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 2166
AoxI GGCC 2 cut(s) 863, 992
ApaLI GTGCAC 1 cut(s) 1816
ApeKI GCWGC 5 cut(s) 1229, 1452, 1455, 1733, 2033
ApoI RAATTY 4 cut(s) 233, 317, 506, 794
AseI ATTAAT 1 cut(s) 1521
AspLEI GCGC 2 cut(s) 194, 843
AspS9I GGNCC 4 cut(s) 992, 1099, 1828, 1926
AsuHPI GGTGA 4 cut(s) 985, 1245, 1693, 2026
AsuII TTCGAA 2 cut(s) 237, 1940
AsuNHI GCTAGC 1 cut(s) 821
AvaI CYCGRG 1 cut(s) 2166
AvaII GGWCC 3 cut(s) 1099, 1828, 1926
AxyI CCTNAGG 1 cut(s) 1388
BaeGI GKGCMC 1 cut(s) 1820
BanI GGYRCC 1 cut(s) 265
BbsI GAAGAC 2 cut(s) 257, 1500
Bbv12I GWGCWC 2 cut(s) 1006, 1820
BbvI GCAGC 5 cut(s) 1241, 1464, 1467, 1720, 2045
BccI CCATC 7 cut(s) 40, 601, 709, 1756, 1882, 1963, 1994
BceAI ACGGC 2 cut(s) 687, 1793
BcgI CGANNNNNNTGC 4 cut(s) 800, 834, 1890, 1924
BciT130I CCWGG 3 cut(s) 501, 867, 1322
BclI TGATCA 1 cut(s) 1416
BcoDI GTCTC 2 cut(s) 1041, 1433
BcuI ACTAGT 1 cut(s) 625
BfaI CTAG 6 cut(s) 626, 822, 1148, 1208, 1377, 1508
BfmI CTRYAG 3 cut(s) 110, 338, 678
BfrI CTTAAG 1 cut(s) 1343
BglI GCCNNNNNGGC 1 cut(s) 871
BglII AGATCT 1 cut(s) 114
BisI GCNGC 7 cut(s) 397, 1230, 1453, 1456, 1734, 1965, 2034
BlsI GCNGC 7 cut(s) 398, 1231, 1454, 1457, 1735, 1966, 2035
BmcAI AGTACT 1 cut(s) 1644
Bme1390I CCNGG 3 cut(s) 501, 867, 1322
Bme18I GGWCC 3 cut(s) 1099, 1828, 1926
BmeT110I CYCGRG 1 cut(s) 2166
BmgBI CACGTC 1 cut(s) 808
BmgT120I GGNCC 4 cut(s) 992, 1099, 1828, 1926
BmiI GGNNCC 6 cut(s) 267, 621, 692, 1100, 1223, 2010
BmrFI CCNGG 3 cut(s) 501, 867, 1322
BmtI GCTAGC 1 cut(s) 825
BpiI GAAGAC 2 cut(s) 257, 1500
BpmI CTGGAG 3 cut(s) 933, 1085, 1343
Bpu14I TTCGAA 2 cut(s) 237, 1940
BpuEI CTTGAG 1 cut(s) 1408
BsaHI GRCGYC 2 cut(s) 528, 674
BsaJI CCNNGG 5 cut(s) 330, 995, 1119, 1628, 1824
BsaWI WCCGGW 2 cut(s) 211, 2011
BsaXI ACNNNNNCTCC 6 cut(s) 1115, 1145, 1302, 1332, 1459, 1489
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 955, 1120
Bse118I RCCGGY 2 cut(s) 268, 687
Bse1I ACTGG 2 cut(s) 207, 2138
Bse21I CCTNAGG 1 cut(s) 1388
Bse3DI GCAATG 3 cut(s) 165, 1629, 1807
BseBI CCWGG 3 cut(s) 501, 867, 1322
BseDI CCNNGG 5 cut(s) 330, 995, 1119, 1628, 1824
BseGI GGATG 5 cut(s) 262, 612, 701, 964, 1363
BseLI CCNNNNNNNGG 3 cut(s) 144, 955, 1120
BseMI GCAATG 3 cut(s) 165, 1629, 1807
BseMII CTCAG 3 cut(s) 30, 1065, 1235
BseNI ACTGG 2 cut(s) 207, 2138
BseRI GAGGAG 4 cut(s) 44, 476, 1340, 1601
BseSI GKGCMC 1 cut(s) 1820
BseXI GCAGC 5 cut(s) 1241, 1464, 1467, 1720, 2045
BsgI GTGCAG 1 cut(s) 1913
BshFI GGCC 2 cut(s) 865, 994
BshNI GGYRCC 1 cut(s) 265
BsiHKAI GWGCWC 2 cut(s) 1006, 1820
BsiHKCI CYCGRG 1 cut(s) 2166
BsiSI CCGG 4 cut(s) 212, 269, 688, 2012
BslFI GGGAC 2 cut(s) 515, 1112
BslI CCNNNNNNNGG 3 cut(s) 144, 955, 1120
BsmAI GTCTC 2 cut(s) 1041, 1433
BsmFI GGGAC 2 cut(s) 515, 1112
BsmI GAATGC 2 cut(s) 1192, 1875
BsnI GGCC 2 cut(s) 865, 994
BsoBI CYCGRG 1 cut(s) 2166
Bsp119I TTCGAA 2 cut(s) 237, 1940
Bsp1286I GDGCHC 2 cut(s) 1006, 1820
Bsp143I GATC 2 cut(s) 114, 1416
Bsp19I CCATGG 1 cut(s) 995
BspACI CCGC 8 cut(s) 397, 1096, 1334, 1359, 1482, 1821, 1964, 2003
BspANI GGCC 2 cut(s) 865, 994
BspCNI CTCAG 3 cut(s) 29, 1066, 1236
BspLI GGNNCC 6 cut(s) 267, 621, 692, 1100, 1223, 2010
BspMAI CTGCAG 2 cut(s) 114, 342
BspOI GCTAGC 1 cut(s) 825
BspT104I TTCGAA 2 cut(s) 237, 1940
BspT107I GGYRCC 1 cut(s) 265
BspTI CTTAAG 1 cut(s) 1343
BsrDI GCAATG 3 cut(s) 165, 1629, 1807
BsrFI RCCGGY 2 cut(s) 268, 687
BsrI ACTGG 2 cut(s) 207, 2138
BssAI RCCGGY 2 cut(s) 268, 687
BssECI CCNNGG 5 cut(s) 330, 995, 1119, 1628, 1824
BssMI GATC 2 cut(s) 114, 1416
BssNI GRCGYC 2 cut(s) 528, 674
BssT1I CCWWGG 4 cut(s) 330, 995, 1119, 1628
Bst2UI CCWGG 3 cut(s) 501, 867, 1322
Bst4CI ACNGT 8 cut(s) 265, 290, 973, 1169, 1499, 1662, 1678, 1879
Bst6I CTCTTC 2 cut(s) 480, 2059
BstACI GRCGYC 2 cut(s) 528, 674
BstAFI CTTAAG 1 cut(s) 1343
BstAPI GCANNNNNTGC 2 cut(s) 1733, 1879
BstBI TTCGAA 2 cut(s) 237, 1940
BstDEI CTNAG 5 cut(s) 16, 1074, 1244, 1388, 2114
BstDSI CCRYGG 1 cut(s) 995
BstENI CCTNNNNNAGG 1 cut(s) 1118
BstF5I GGATG 5 cut(s) 262, 612, 701, 964, 1363
BstHHI GCGC 2 cut(s) 194, 843
BstKTI GATC 2 cut(s) 117, 1419
BstMAI GTCTC 2 cut(s) 1041, 1433
BstMBI GATC 2 cut(s) 114, 1416
BstMWI GCNNNNNNNGC 5 cut(s) 838, 871, 1733, 1879, 1964
BstNI CCWGG 3 cut(s) 501, 867, 1322
BstNSI RCATGY 3 cut(s) 355, 761, 1958
BstSCI CCNGG 3 cut(s) 499, 865, 1320
BstSFI CTRYAG 3 cut(s) 110, 338, 678
BstSLI GKGCMC 1 cut(s) 1820
BstV1I GCAGC 5 cut(s) 1241, 1464, 1467, 1720, 2045
BstV2I GAAGAC 2 cut(s) 257, 1500
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
Bsu36I CCTNAGG 1 cut(s) 1388
BsuRI GGCC 2 cut(s) 865, 994
BtgI CCRYGG 1 cut(s) 995
BtgZI GCGATG 2 cut(s) 1972, 1982
BtrI CACGTC 1 cut(s) 808
BtsCI GGATG 5 cut(s) 262, 612, 701, 964, 1363
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 3 cut(s) 331, 1603, 1674
CaiI CAGNNNCTG 1 cut(s) 23
CfoI GCGC 2 cut(s) 194, 843
Cfr10I RCCGGY 2 cut(s) 268, 687
Cfr13I GGNCC 4 cut(s) 992, 1099, 1828, 1926
CseI GACGC 1 cut(s) 663
Csp6I GTAC 4 cut(s) 182, 1643, 1836, 2079
CspCI CAANNNNNGTGG 2 cut(s) 942, 977
CviQI GTAC 4 cut(s) 182, 1643, 1836, 2079
DdeI CTNAG 5 cut(s) 16, 1074, 1244, 1388, 2114
DpnI GATC 2 cut(s) 116, 1418
DpnII GATC 2 cut(s) 114, 1416
DraI TTTAAA 1 cut(s) 720
DrdI GACNNNNNNGTC 1 cut(s) 2170
DseDI GACNNNNNNGTC 1 cut(s) 2170
Eam1104I CTCTTC 2 cut(s) 480, 2059
EarI CTCTTC 2 cut(s) 480, 2059
Eco130I CCWWGG 4 cut(s) 330, 995, 1119, 1628
Eco47I GGWCC 3 cut(s) 1099, 1828, 1926
Eco81I CCTNAGG 1 cut(s) 1388
Eco88I CYCGRG 1 cut(s) 2166
EcoNI CCTNNNNNAGG 1 cut(s) 1118
EcoRI GAATTC 2 cut(s) 233, 794
EcoRII CCWGG 3 cut(s) 499, 865, 1320
EcoT14I CCWWGG 4 cut(s) 330, 995, 1119, 1628
EcoT22I ATGCAT 3 cut(s) 598, 1140, 1194
ErhI CCWWGG 4 cut(s) 330, 995, 1119, 1628
FalI AAGNNNNNCTT 2 cut(s) 921, 953
FaqI GGGAC 2 cut(s) 515, 1112
FauI CCCGC 2 cut(s) 1089, 1352
FbaI TGATCA 1 cut(s) 1416
FblI GTMKAC 1 cut(s) 677
Fnu4HI GCNGC 7 cut(s) 397, 1230, 1453, 1456, 1734, 1965, 2034
FokI GGATG 5 cut(s) 269, 619, 688, 971, 1350
Fsp4HI GCNGC 7 cut(s) 397, 1230, 1453, 1456, 1734, 1965, 2034
FspBI CTAG 6 cut(s) 626, 822, 1148, 1208, 1377, 1508
GlaI GCGC 2 cut(s) 193, 842
GluI GCNGC 7 cut(s) 397, 1230, 1453, 1456, 1734, 1965, 2034
GsuI CTGGAG 3 cut(s) 933, 1085, 1343
HaeIII GGCC 2 cut(s) 865, 994
HapII CCGG 4 cut(s) 212, 269, 688, 2012
HgaI GACGC 1 cut(s) 663
HhaI GCGC 2 cut(s) 194, 843
Hin1I GRCGYC 2 cut(s) 528, 674
Hin6I GCGC 2 cut(s) 192, 841
HinP1I GCGC 2 cut(s) 192, 841
HincII GTYRAC 1 cut(s) 1665
HindII GTYRAC 1 cut(s) 1665
HinfI GANTC 8 cut(s) 452, 1034, 1339, 1409, 1593, 2129, 2164, 2171
HpaII CCGG 4 cut(s) 212, 269, 688, 2012
HphI GGTGA 4 cut(s) 985, 1245, 1693, 2026
Hpy166II GTNNAC 4 cut(s) 138, 678, 1665, 1818
Hpy188I TCNGA 5 cut(s) 517, 547, 1051, 1173, 1828
Hpy188III TCNNGA 8 cut(s) 912, 1040, 1064, 1436, 1709, 2070, 2161, 2168
Hpy8I GTNNAC 4 cut(s) 138, 678, 1665, 1818
Hpy99I CGWCG 3 cut(s) 449, 794, 812
HpyAV CCTTC 8 cut(s) 48, 54, 68, 469, 763, 1300, 1342, 1936
HpyCH4III ACNGT 8 cut(s) 265, 290, 973, 1169, 1499, 1662, 1678, 1879
HpyCH4IV ACGT 3 cut(s) 528, 807, 923
HpyF10VI GCNNNNNNNGC 5 cut(s) 838, 871, 1733, 1879, 1964
HpyF3I CTNAG 5 cut(s) 16, 1074, 1244, 1388, 2114
HpySE526I ACGT 3 cut(s) 528, 807, 923
Hsp92I GRCGYC 2 cut(s) 528, 674
HspAI GCGC 2 cut(s) 192, 841
Ksp22I TGATCA 1 cut(s) 1416
Kzo9I GATC 2 cut(s) 114, 1416
LmnI GCTCC 8 cut(s) 517, 696, 778, 964, 1009, 1227, 1327, 1392
Lsp1109I GCAGC 5 cut(s) 1241, 1464, 1467, 1720, 2045
MaeI CTAG 6 cut(s) 626, 822, 1148, 1208, 1377, 1508
MaeII ACGT 3 cut(s) 528, 807, 923
MaeIII GTNAC 3 cut(s) 347, 628, 1699
MalI GATC 2 cut(s) 116, 1418
MboI GATC 2 cut(s) 114, 1416
MflI RGATCY 1 cut(s) 114
MhlI GDGCHC 2 cut(s) 1006, 1820
MlyI GAGTC 5 cut(s) 1043, 1348, 1418, 2158, 2180
MmeI TCCRAC 1 cut(s) 565
Mph1103I ATGCAT 3 cut(s) 598, 1140, 1194
MseI TTAA 9 cut(s) 668, 719, 920, 939, 1277, 1344, 1521, 1581, 1694
MslI CAYNNNNRTG 2 cut(s) 1959, 2027
MspCI CTTAAG 1 cut(s) 1343
MspI CCGG 4 cut(s) 212, 269, 688, 2012
MspR9I CCNGG 3 cut(s) 501, 867, 1322
Mva1269I GAATGC 2 cut(s) 1192, 1875
MvaI CCWGG 3 cut(s) 501, 867, 1322
MwoI GCNNNNNNNGC 5 cut(s) 838, 871, 1733, 1879, 1964
NcoI CCATGG 1 cut(s) 995
NdeII GATC 2 cut(s) 114, 1416
NheI GCTAGC 1 cut(s) 821
NlaIV GGNNCC 6 cut(s) 267, 621, 692, 1100, 1223, 2010
NmuCI GTSAC 2 cut(s) 347, 1699
NsiI ATGCAT 3 cut(s) 598, 1140, 1194
NspI RCATGY 3 cut(s) 355, 761, 1958
NspV TTCGAA 2 cut(s) 237, 1940
PaeI GCATGC 1 cut(s) 1958
PaeR7I CTCGAG 1 cut(s) 2166
PciI ACATGT 1 cut(s) 351
PctI GAATGC 2 cut(s) 1192, 1875
PfeI GAWTC 3 cut(s) 452, 1593, 2129
PfoI TCCNGGA 1 cut(s) 1320
PkrI GCNGC 7 cut(s) 398, 1231, 1454, 1457, 1735, 1966, 2035
PleI GAGTC 5 cut(s) 1042, 1347, 1417, 2158, 2179
PpsI GAGTC 5 cut(s) 1042, 1347, 1417, 2158, 2179
PscI ACATGT 1 cut(s) 351
PshBI ATTAAT 1 cut(s) 1521
PsiI TTATAA 1 cut(s) 2027
Psp6I CCWGG 3 cut(s) 499, 865, 1320
PspGI CCWGG 3 cut(s) 499, 865, 1320
PspN4I GGNNCC 6 cut(s) 267, 621, 692, 1100, 1223, 2010
PspPI GGNCC 4 cut(s) 992, 1099, 1828, 1926
PsrI GAACNNNNNNTAC 2 cut(s) 1363, 1395
PstI CTGCAG 2 cut(s) 114, 342
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 1 cut(s) 114
RsaI GTAC 4 cut(s) 183, 1644, 1837, 2080
RsaNI GTAC 4 cut(s) 182, 1643, 1836, 2079
RseI CAYNNNNRTG 2 cut(s) 1959, 2027
SaqAI TTAA 9 cut(s) 668, 719, 920, 939, 1277, 1344, 1521, 1581, 1694
SatI GCNGC 7 cut(s) 397, 1230, 1453, 1456, 1734, 1965, 2034
Sau3AI GATC 2 cut(s) 114, 1416
Sau96I GGNCC 4 cut(s) 992, 1099, 1828, 1926
ScaI AGTACT 1 cut(s) 1644
SchI GAGTC 5 cut(s) 1043, 1348, 1418, 2158, 2180
ScrFI CCNGG 3 cut(s) 501, 867, 1322
SduI GDGCHC 2 cut(s) 1006, 1820
SfcI CTRYAG 3 cut(s) 110, 338, 678
Sfr274I CTCGAG 1 cut(s) 2166
SfuI TTCGAA 2 cut(s) 237, 1940
SinI GGWCC 3 cut(s) 1099, 1828, 1926
SlaI CTCGAG 1 cut(s) 2166
SmiMI CAYNNNNRTG 2 cut(s) 1959, 2027
SmlI CTYRAG 3 cut(s) 1343, 1423, 2166
SmoI CTYRAG 3 cut(s) 1343, 1423, 2166
SpeI ACTAGT 1 cut(s) 625
SphI GCATGC 1 cut(s) 1958
SsiI CCGC 8 cut(s) 397, 1096, 1334, 1359, 1482, 1821, 1964, 2003
SspI AATATT 2 cut(s) 716, 1995
SspMI CTAG 6 cut(s) 626, 822, 1148, 1208, 1377, 1508
StyD4I CCNGG 3 cut(s) 499, 865, 1320
StyI CCWWGG 4 cut(s) 330, 995, 1119, 1628
TaaI ACNGT 8 cut(s) 265, 290, 973, 1169, 1499, 1662, 1678, 1879
TaiI ACGT 3 cut(s) 531, 810, 926
TatI WGTACW 2 cut(s) 1642, 1835
TauI GCSGC 2 cut(s) 399, 1967
TfiI GAWTC 3 cut(s) 452, 1593, 2129
Tru1I TTAA 9 cut(s) 668, 719, 920, 939, 1277, 1344, 1521, 1581, 1694
Tru9I TTAA 9 cut(s) 668, 719, 920, 939, 1277, 1344, 1521, 1581, 1694
TscAI CASTG 3 cut(s) 331, 1603, 1681
TseFI GTSAC 2 cut(s) 347, 1699
TseI GCWGC 5 cut(s) 1229, 1452, 1455, 1733, 2033
Tsp45I GTSAC 2 cut(s) 347, 1699
TspDTI ATGAA 6 cut(s) 273, 787, 942, 1412, 1732, 2011
TspGWI ACGGA 2 cut(s) 269, 2141
TspRI CASTG 3 cut(s) 331, 1603, 1681
Vha464I CTTAAG 1 cut(s) 1343
VneI GTGCAC 1 cut(s) 1816
VpaK11BI GGWCC 3 cut(s) 1099, 1828, 1926
VspI ATTAAT 1 cut(s) 1521
XagI CCTNNNNNAGG 1 cut(s) 1118
XapI RAATTY 4 cut(s) 233, 317, 506, 794
XceI RCATGY 3 cut(s) 355, 761, 1958
XcmI CCANNNNNNNNNTGG 1 cut(s) 1675
XhoI CTCGAG 1 cut(s) 2166
XmiI GTMKAC 1 cut(s) 677
XspI CTAG 6 cut(s) 626, 822, 1148, 1208, 1377, 1508
ZraI GACGTC 1 cut(s) 529
ZrmI AGTACT 1 cut(s) 1644
Zsp2I ATGCAT 3 cut(s) 598, 1140, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.