Rroxscaffold_6G00389250

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012209
Physical Location & Seq
Forward (+)
2653176 .. 2657853
4678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00389250.1

Sequence Viewer

Length: 2094 bp
ATGTCGACAATGAGAATGAAACATCTCTCCTCCGTTGCCAAGGATGTTGTACAATTCTGCGCTGTGAAACTGAACACATCGGTGGATAAGTTAACGGAGGAATTTGAAGCCACCAGATCATGGAACAACAAAACGGCGTCGTCACCCGGTGGCGGTGGTGCTGCTGCTGTTGGCACTTACGCGAGGAAATTTATAGAATTTTGCAGTGCAAAGGGTGTGAGTGAGTTATGCCAAAACAATATTGAGGAAAAGATTGCCGATGGAATCTTTAGTCGTTTCACATTCGACATGATGCTCGCCTGGGAGATTCCCGAATCTGCCCAAGAGGACTCTTTCACCGAGTGTGTGGCAAAGGAGAAAGAAGACAAAAATGTAGTTGCAAAGCTTCCTCCAGAACAAGATGATGTTTCTTTGTTCTATACAGACCTCATGCCACTTCTAGTTGATAATGAGCGGAACATTGGAGAAGATGCATTTGTGTGGTTGGGATCACTTGTTCCATTAGTCACAGATGTTGTTAATGGAAGATTTACCTTTGAAACTTTAACAGCACCTACAGGATACCGGCTCCATTTTCCTGCGTATGACAAATACCTCAAGGAAATCGACAAATGTATAAAACATCTGCAAAAACAAGCAAAGCCAAAGGGTGTGGAGCTTGCAGATGATGAATTCATTTTACATGTGGAGGGAACTGCTAGCACACAAAGAGTGGTACGCCACATCGGAGGAACAAGTTGGCCTGGTAAGCTCACCCTGACCAATTACGCACTCTATTTTGAGGCCTCTGGTGTGATAACATATGAAGATGCTCTTAAAATAGACCTTTCAAAGGATAAGGAGCAGAGTGTCAAACCAGCCTCTACAGGACCATGGGGTGCCCCACTTTTTGACAAGGCTATTGTTATCGAGTCTCCAGAGTTATCAGAGGGGATTGTGCTCGAGTTCCCCGAGGTGACAAGCTCCACAAGGCGTGACCATTGGCTTGCACTAACAAAGGAGATCATGTTGATGCACCAGTTTCTATCAAAATTCAAATTAGAATGTCCAATACAAGCATGGGAGATGCATGCAAGGACAATACTATCAATAATAAGGCTCCATGCAGCCAGGGAAATGCTAAGAATATCTCCCCCTCAACCGACACGATTCTTAATCTTTTCCTTGTTTGAGGAGTTACCTAAGGGGGATTACATTCTAGAAGAGCTTGCTGAGAGTCTAAAGAAGGTCAATAGTGGACATCCATGTAGTGCAAGTTCAATCCTAAGGAGTATGATCATTTCTGAGTCAATTCACTCAAGTGTAGAGGTAACAGAAGTGGGAAAGGAAATTGCAGGTCCAAGTGGTCCAGTTGAAGACACATCCTTATTAGAAAGTGTAATTAATCAAGCAAGAGAGGAAGAGAAGGAAATCGCTATTGCCAAAGCGACCACAGAGGGACTGAAAGAAGAAGGCATCACTGAAAGCACTACTATTTTTATGGTATTCCCCTTAGAAATGAGTGGTCTTGGTGAAGAAATCCTCACATGGGAAAGGCCAGCAACCACACTGATTGTGATTGGTGCGGCTCTAATTGTCACCTACAAGGAGTGGGTGGGCAAAGCCATAGCAGCTTTCTTGGTCTGGGTGGTTGCAAAGATGATTAGAGCAAGACAGGAAAGAATTGATGAAAAGTGCAATGAGCTAGTTGTGTACACTGCATCTGAACAGACTACAGTTGAAAACATAGTGTCAGCTCAACATGGGGTGCAAACCGTGCATGAGATAGTGAAGAAAGCAAATGTCTCGGTGTTGAAACTGTGGTCGATATTCATTTCAAAGGCCCACAAGCATGCAAATACAGCAATGCTGGTGCTAGGTGCAGCCTCAATATTTTTAGCGGTGGTTCCGATGAAGGTGTTTATAATGGGAGCCTTGTTGTATTACTTCACAATGACATTAGCATCAAAGTTCGGTAAGAGCGAGGGGAACAACCAAGGATTTAATAGACGACTGAAAGAATGGTGGGATTCAATTCCAGTTATTCCTGTGCATGTTGTTGATGAGGTTCCCAACAGTCCTAATCGAATGAAATGCATACAGGCGGAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

697

Amino Acids

77.63

Weight (kDa)

5.47

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 456 - 682 3.9e-55 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21720 AT2G21720
fragaria_vesca FvH4_1g28470 FvH4_1g28470 FvH4_1g28470
malus_domestica MD12G1008600.v1.1 MD14G1006000.v1.1
prunus_persica Prupe.7G010400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0496361 RchiOBHm_Chr3g0496371
rosa_laevigata RLG00000022528
rosa_multiflora Rmu_sc0002461.1_g000006
rosa_roxburghii Rroxscaffold_6G00389250
rosa_rugosa Rorug03G0282000
rosa_samantha Rh3AG328600 Rh3BG364700 Rh3CG361700 Rh3DG364700
rosa_wichuraiana Rw3G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1904
Acc36I ACCTGC 1 cut(s) 1325
AccB1I GGYRCC 1 cut(s) 878
AccB7I CCANNNNNTGG 1 cut(s) 120
AccBSI CCGCTC 1 cut(s) 454
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 182
AciI CCGC 5 cut(s) 153, 454, 1565, 1880, 2084
AclWI GGATC 1 cut(s) 496
AcsI RAATTY 5 cut(s) 101, 188, 197, 671, 1031
AcyI GRCGYC 1 cut(s) 137
AdeI CACNNNGTG 2 cut(s) 149, 342
AfaI GTAC 3 cut(s) 51, 717, 1694
AfiI CCNNNNNNNGG 4 cut(s) 120, 152, 832, 1528
AflIII ACRYGT 1 cut(s) 682
AjnI CCWGG 3 cut(s) 299, 742, 1109
AleI CACNNNNGTG 2 cut(s) 80, 1299
AloI GAACNNNNNNTCC 2 cut(s) 480, 512
AluBI AGCT 8 cut(s) 385, 658, 751, 963, 1207, 1613, 1684, 1736
AluI AGCT 8 cut(s) 385, 658, 751, 963, 1207, 1613, 1684, 1736
Alw21I GWGCWC 1 cut(s) 942
Alw26I GTCTC 2 cut(s) 918, 1789
AlwI GGATC 1 cut(s) 496
Ama87I CYCGRG 2 cut(s) 941, 950
AoxI GGCC 4 cut(s) 740, 783, 1535, 1821
ApeKI GCWGC 5 cut(s) 161, 164, 1106, 1610, 1862
ApoI RAATTY 5 cut(s) 101, 188, 197, 671, 1031
AseI ATTAAT 1 cut(s) 1383
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 4 cut(s) 869, 1337, 1346, 1822
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 6 cut(s) 135, 328, 745, 967, 1523, 1570
AsuNHI GCTAGC 1 cut(s) 698
AvaI CYCGRG 2 cut(s) 941, 950
AvaII GGWCC 3 cut(s) 869, 1337, 1346
AxyI CCTNAGG 2 cut(s) 1182, 1265
BaeGI GKGCMC 1 cut(s) 883
BanI GGYRCC 1 cut(s) 878
BbsI GAAGAC 2 cut(s) 369, 1362
Bbv12I GWGCWC 1 cut(s) 942
BbvI GCAGC 5 cut(s) 148, 151, 1118, 1622, 1874
BccI CCATC 1 cut(s) 254
BceAI ACGGC 1 cut(s) 150
BcgI CGANNNNNNTGC 4 cut(s) 1767, 1801, 2055, 2089
BciT130I CCWGG 3 cut(s) 301, 744, 1111
BciVI GTATCC 1 cut(s) 554
BclI TGATCA 1 cut(s) 1275
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 918, 1789
BfaI CTAG 6 cut(s) 440, 699, 1199, 1685, 1856, 2092
BfmI CTRYAG 3 cut(s) 555, 864, 1713
BfuAI ACCTGC 1 cut(s) 1325
BfuI GTATCC 1 cut(s) 554
BisI GCNGC 6 cut(s) 162, 165, 1107, 1566, 1611, 1863
BlsI GCNGC 6 cut(s) 163, 166, 1108, 1567, 1612, 1864
Bme1390I CCNGG 4 cut(s) 147, 301, 744, 1111
Bme18I GGWCC 3 cut(s) 869, 1337, 1346
BmeT110I CYCGRG 2 cut(s) 941, 950
BmgT120I GGNCC 4 cut(s) 869, 1337, 1346, 1822
BmiI GGNNCC 6 cut(s) 569, 880, 1100, 1887, 1912, 2049
BmrFI CCNGG 4 cut(s) 147, 301, 744, 1111
BmsI GCATC 8 cut(s) 282, 460, 799, 1002, 1056, 1464, 1709, 1952
BmtI GCTAGC 1 cut(s) 702
BpiI GAAGAC 2 cut(s) 369, 1362
BpmI CTGGAG 2 cut(s) 375, 900
BpuEI CTTGAG 2 cut(s) 581, 1282
BpuMI CCSGG 1 cut(s) 147
BsaHI GRCGYC 1 cut(s) 137
BsaJI CCNNGG 6 cut(s) 39, 300, 872, 951, 1110, 1975
BsaXI ACNNNNNCTCC 2 cut(s) 992, 1022
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 152, 832, 1528
Bse118I RCCGGY 1 cut(s) 564
Bse1I ACTGG 3 cut(s) 1018, 1349, 2018
Bse21I CCTNAGG 2 cut(s) 1182, 1265
Bse3DI GCAATG 2 cut(s) 1684, 1851
BseBI CCWGG 3 cut(s) 301, 744, 1111
BseDI CCNNGG 6 cut(s) 39, 300, 872, 951, 1110, 1975
BseGI GGATG 3 cut(s) 49, 1240, 1361
BseLI CCNNNNNNNGG 4 cut(s) 120, 152, 832, 1528
BseMI GCAATG 2 cut(s) 1684, 1851
BseMII CTCAG 2 cut(s) 1203, 1275
BseNI ACTGG 3 cut(s) 1018, 1349, 2018
BseRI GAGGAG 2 cut(s) 19, 1187
BseSI GKGCMC 1 cut(s) 883
BseXI GCAGC 5 cut(s) 148, 151, 1118, 1622, 1874
BsgI GTGCAG 1 cut(s) 1881
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 4 cut(s) 742, 785, 1537, 1823
BshNI GGYRCC 1 cut(s) 878
BsiHKAI GWGCWC 1 cut(s) 942
BsiHKCI CYCGRG 2 cut(s) 941, 950
BsiSI CCGG 2 cut(s) 147, 565
BslFI GGGAC 1 cut(s) 1452
BslI CCNNNNNNNGG 4 cut(s) 120, 152, 832, 1528
BsmAI GTCTC 2 cut(s) 918, 1789
BsmFI GGGAC 1 cut(s) 1452
BsnI GGCC 4 cut(s) 742, 785, 1537, 1823
BsoBI CYCGRG 2 cut(s) 941, 950
Bsp1286I GDGCHC 2 cut(s) 883, 942
Bsp1407I TGTACA 2 cut(s) 49, 1692
Bsp143I GATC 4 cut(s) 116, 488, 1002, 1275
Bsp19I CCATGG 1 cut(s) 872
BspACI CCGC 5 cut(s) 153, 454, 1565, 1880, 2084
BspANI GGCC 4 cut(s) 742, 785, 1537, 1823
BspCNI CTCAG 2 cut(s) 1204, 1276
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 6 cut(s) 569, 880, 1100, 1887, 1912, 2049
BspMI ACCTGC 1 cut(s) 1325
BspOI GCTAGC 1 cut(s) 702
BspPI GGATC 1 cut(s) 496
BspQI GCTCTTC 1 cut(s) 1197
BspT107I GGYRCC 1 cut(s) 878
BsrBI CCGCTC 1 cut(s) 454
BsrDI GCAATG 2 cut(s) 1684, 1851
BsrFI RCCGGY 1 cut(s) 564
BsrGI TGTACA 2 cut(s) 49, 1692
BsrI ACTGG 3 cut(s) 1018, 1349, 2018
BssAI RCCGGY 1 cut(s) 564
BssECI CCNNGG 6 cut(s) 39, 300, 872, 951, 1110, 1975
BssMI GATC 4 cut(s) 116, 488, 1002, 1275
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 3 cut(s) 39, 872, 1975
Bst2UI CCWGG 3 cut(s) 301, 744, 1111
Bst4CI ACNGT 4 cut(s) 1717, 1756, 1800, 2057
Bst6I CTCTTC 2 cut(s) 1197, 1395
BstACI GRCGYC 1 cut(s) 137
BstAPI GCANNNNNTGC 1 cut(s) 1756
BstAUI TGTACA 2 cut(s) 49, 1692
BstC8I GCNNGC 8 cut(s) 297, 660, 700, 987, 1071, 1209, 1539, 1833
BstDEI CTNAG 6 cut(s) 1121, 1182, 1212, 1265, 1284, 1492
BstDSI CCRYGG 1 cut(s) 872
BstENI CCTNNNNNAGG 1 cut(s) 830
BstF5I GGATG 3 cut(s) 49, 1240, 1361
BstFNI CGCG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 4 cut(s) 119, 491, 1005, 1278
BstMAI GTCTC 2 cut(s) 918, 1789
BstMBI GATC 4 cut(s) 116, 488, 1002, 1275
BstMWI GCNNNNNNNGC 4 cut(s) 748, 1610, 1756, 1841
BstNI CCWGG 3 cut(s) 301, 744, 1111
BstNSI RCATGY 4 cut(s) 686, 1073, 1835, 2036
BstSCI CCNGG 4 cut(s) 145, 299, 742, 1109
BstSFI CTRYAG 3 cut(s) 555, 864, 1713
BstSLI GKGCMC 1 cut(s) 883
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 5 cut(s) 148, 151, 1118, 1622, 1874
BstV2I GAAGAC 2 cut(s) 369, 1362
Bsu36I CCTNAGG 2 cut(s) 1182, 1265
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 4 cut(s) 742, 785, 1537, 1823
BtgI CCRYGG 1 cut(s) 872
BtsCI GGATG 3 cut(s) 49, 1240, 1361
BtsI GCAGTG 2 cut(s) 211, 1695
BtsIMutI CAGTG 4 cut(s) 211, 1458, 1547, 1695
BveI ACCTGC 1 cut(s) 1325
Cac8I GCNNGC 8 cut(s) 297, 660, 700, 987, 1071, 1209, 1539, 1833
CfoI GCGC 1 cut(s) 62
Cfr10I RCCGGY 1 cut(s) 564
Cfr13I GGNCC 4 cut(s) 869, 1337, 1346, 1822
CseI GACGC 1 cut(s) 126
Csp6I GTAC 3 cut(s) 50, 716, 1693
CspCI CAANNNNNGTGG 4 cut(s) 633, 668, 1534, 1569
CviQI GTAC 3 cut(s) 50, 716, 1693
DdeI CTNAG 6 cut(s) 1121, 1182, 1212, 1265, 1284, 1492
DpnI GATC 4 cut(s) 118, 490, 1004, 1277
DpnII GATC 4 cut(s) 116, 488, 1002, 1275
DraIII CACNNNGTG 2 cut(s) 149, 342
Eam1104I CTCTTC 2 cut(s) 1197, 1395
EarI CTCTTC 2 cut(s) 1197, 1395
Eco130I CCWWGG 3 cut(s) 39, 872, 1975
Eco147I AGGCCT 1 cut(s) 785
Eco47I GGWCC 3 cut(s) 869, 1337, 1346
Eco81I CCTNAGG 2 cut(s) 1182, 1265
Eco88I CYCGRG 2 cut(s) 941, 950
EcoNI CCTNNNNNAGG 1 cut(s) 830
EcoRI GAATTC 1 cut(s) 671
EcoRII CCWGG 3 cut(s) 299, 742, 1109
EcoT14I CCWWGG 3 cut(s) 39, 872, 1975
EcoT22I ATGCAT 3 cut(s) 475, 1071, 2078
ErhI CCWWGG 3 cut(s) 39, 872, 1975
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FaqI GGGAC 1 cut(s) 1452
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 1 cut(s) 1275
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 162, 165, 1107, 1566, 1611, 1863
FokI GGATG 3 cut(s) 56, 1227, 1348
Fsp4HI GCNGC 6 cut(s) 162, 165, 1107, 1566, 1611, 1863
FspBI CTAG 6 cut(s) 440, 699, 1199, 1685, 1856, 2092
GlaI GCGC 1 cut(s) 61
GluI GCNGC 6 cut(s) 162, 165, 1107, 1566, 1611, 1863
GsuI CTGGAG 2 cut(s) 375, 900
HaeIII GGCC 4 cut(s) 742, 785, 1537, 1823
HapII CCGG 2 cut(s) 147, 565
HgaI GACGC 1 cut(s) 126
HhaI GCGC 1 cut(s) 62
Hin1I GRCGYC 1 cut(s) 137
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HincII GTYRAC 2 cut(s) 6, 93
HindII GTYRAC 2 cut(s) 6, 93
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 9 cut(s) 264, 307, 314, 329, 911, 1149, 1216, 1286, 2009
HpaI GTTAAC 1 cut(s) 93
HpaII CCGG 2 cut(s) 147, 565
HphI GGTGA 6 cut(s) 135, 328, 745, 967, 1523, 1570
Hpy166II GTNNAC 5 cut(s) 6, 93, 1238, 1693, 1695
Hpy188I TCNGA 5 cut(s) 728, 928, 1285, 1705, 1890
Hpy188III TCNNGA 4 cut(s) 311, 392, 917, 1199
Hpy8I GTNNAC 5 cut(s) 6, 93, 1238, 1693, 1695
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 5 cut(s) 1219, 1399, 1445, 1888, 2081
HpyCH4III ACNGT 4 cut(s) 1717, 1756, 1800, 2057
HpyF10VI GCNNNNNNNGC 4 cut(s) 748, 1610, 1756, 1841
HpyF3I CTNAG 6 cut(s) 1121, 1182, 1212, 1265, 1284, 1492
Hsp92I GRCGYC 1 cut(s) 137
HspAI GCGC 1 cut(s) 60
Ksp22I TGATCA 1 cut(s) 1275
KspAI GTTAAC 1 cut(s) 93
Kzo9I GATC 4 cut(s) 116, 488, 1002, 1275
LguI GCTCTTC 1 cut(s) 1197
LmnI GCTCC 6 cut(s) 573, 655, 841, 968, 1104, 1910
Lsp1109I GCAGC 5 cut(s) 148, 151, 1118, 1622, 1874
LweI GCATC 8 cut(s) 282, 460, 799, 1002, 1056, 1464, 1709, 1952
MaeI CTAG 6 cut(s) 440, 699, 1199, 1685, 1856, 2092
MaeIII GTNAC 7 cut(s) 141, 505, 955, 974, 1176, 1309, 1576
MalI GATC 4 cut(s) 118, 490, 1004, 1277
MbiI CCGCTC 1 cut(s) 454
MboI GATC 4 cut(s) 116, 488, 1002, 1275
MhlI GDGCHC 2 cut(s) 883, 942
MlyI GAGTC 4 cut(s) 323, 920, 1225, 1295
Mph1103I ATGCAT 3 cut(s) 475, 1071, 2078
MseI TTAA 7 cut(s) 92, 519, 545, 816, 1154, 1383, 1983
MslI CAYNNNNRTG 5 cut(s) 80, 478, 1010, 1299, 1830
MspI CCGG 2 cut(s) 147, 565
MspR9I CCNGG 4 cut(s) 147, 301, 744, 1111
MvaI CCWGG 3 cut(s) 301, 744, 1111
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 4 cut(s) 748, 1610, 1756, 1841
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 872
NdeI CATATG 1 cut(s) 802
NdeII GATC 4 cut(s) 116, 488, 1002, 1275
NheI GCTAGC 1 cut(s) 698
NlaIV GGNNCC 6 cut(s) 569, 880, 1100, 1887, 1912, 2049
NmuCI GTSAC 5 cut(s) 141, 505, 955, 974, 1576
NsiI ATGCAT 3 cut(s) 475, 1071, 2078
NspI RCATGY 4 cut(s) 686, 1073, 1835, 2036
OliI CACNNNNGTG 2 cut(s) 80, 1299
PaeI GCATGC 2 cut(s) 1073, 1835
PaeR7I CTCGAG 1 cut(s) 941
PceI AGGCCT 1 cut(s) 785
PciI ACATGT 1 cut(s) 682
PciSI GCTCTTC 1 cut(s) 1197
PcsI WCGNNNNNNNCGW 2 cut(s) 948, 1959
PfeI GAWTC 5 cut(s) 264, 307, 314, 1149, 2009
PflMI CCANNNNNTGG 1 cut(s) 120
PkrI GCNGC 6 cut(s) 163, 166, 1108, 1567, 1612, 1864
PleI GAGTC 4 cut(s) 323, 919, 1224, 1294
PpsI GAGTC 4 cut(s) 323, 919, 1224, 1294
PscI ACATGT 1 cut(s) 682
PshBI ATTAAT 1 cut(s) 1383
PsiI TTATAA 1 cut(s) 1904
Psp6I CCWGG 3 cut(s) 299, 742, 1109
PspGI CCWGG 3 cut(s) 299, 742, 1109
PspN4I GGNNCC 6 cut(s) 569, 880, 1100, 1887, 1912, 2049
PspPI GGNCC 4 cut(s) 869, 1337, 1346, 1822
PspXI VCTCGAGB 1 cut(s) 941
PsrI GAACNNNNNNTAC 2 cut(s) 1240, 1272
RsaI GTAC 3 cut(s) 51, 717, 1694
RsaNI GTAC 3 cut(s) 50, 716, 1693
RseI CAYNNNNRTG 5 cut(s) 80, 478, 1010, 1299, 1830
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 1197
SaqAI TTAA 7 cut(s) 92, 519, 545, 816, 1154, 1383, 1983
SatI GCNGC 6 cut(s) 162, 165, 1107, 1566, 1611, 1863
Sau3AI GATC 4 cut(s) 116, 488, 1002, 1275
Sau96I GGNCC 4 cut(s) 869, 1337, 1346, 1822
SchI GAGTC 4 cut(s) 323, 920, 1225, 1295
ScrFI CCNGG 4 cut(s) 147, 301, 744, 1111
SduI GDGCHC 2 cut(s) 883, 942
SfaNI GCATC 8 cut(s) 282, 460, 799, 1002, 1056, 1464, 1709, 1952
SfcI CTRYAG 3 cut(s) 555, 864, 1713
Sfr274I CTCGAG 1 cut(s) 941
SinI GGWCC 3 cut(s) 869, 1337, 1346
SlaI CTCGAG 1 cut(s) 941
SmiMI CAYNNNNRTG 5 cut(s) 80, 478, 1010, 1299, 1830
SmlI CTYRAG 3 cut(s) 596, 941, 1297
SmoI CTYRAG 3 cut(s) 596, 941, 1297
SphI GCATGC 2 cut(s) 1073, 1835
SseBI AGGCCT 1 cut(s) 785
SsiI CCGC 5 cut(s) 153, 454, 1565, 1880, 2084
SspI AATATT 2 cut(s) 241, 1872
SspMI CTAG 6 cut(s) 440, 699, 1199, 1685, 1856, 2092
StuI AGGCCT 1 cut(s) 785
StyD4I CCNGG 4 cut(s) 145, 299, 742, 1109
StyI CCWWGG 3 cut(s) 39, 872, 1975
TaaI ACNGT 4 cut(s) 1717, 1756, 1800, 2057
TaqI TCGA 7 cut(s) 5, 285, 606, 909, 942, 1805, 2065
TatI WGTACW 2 cut(s) 49, 1692
TauI GCSGC 1 cut(s) 1568
TfiI GAWTC 5 cut(s) 264, 307, 314, 1149, 2009
Tru1I TTAA 7 cut(s) 92, 519, 545, 816, 1154, 1383, 1983
Tru9I TTAA 7 cut(s) 92, 519, 545, 816, 1154, 1383, 1983
TscAI CASTG 4 cut(s) 211, 1465, 1554, 1702
TseFI GTSAC 5 cut(s) 141, 505, 955, 974, 1576
TseI GCWGC 5 cut(s) 161, 164, 1106, 1610, 1862
Tsp45I GTSAC 5 cut(s) 141, 505, 955, 974, 1576
TspDTI ATGAA 8 cut(s) 32, 664, 684, 819, 1683, 1801, 1907, 2084
TspGWI ACGGA 2 cut(s) 22, 110
TspRI CASTG 4 cut(s) 211, 1465, 1554, 1702
Van91I CCANNNNNTGG 1 cut(s) 120
VpaK11BI GGWCC 3 cut(s) 869, 1337, 1346
VspI ATTAAT 1 cut(s) 1383
XagI CCTNNNNNAGG 1 cut(s) 830
XapI RAATTY 5 cut(s) 101, 188, 197, 671, 1031
XbaI TCTAGA 1 cut(s) 1198
XceI RCATGY 4 cut(s) 686, 1073, 1835, 2036
XcmI CCANNNNNNNNNTGG 1 cut(s) 1056
XhoI CTCGAG 1 cut(s) 941
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 6 cut(s) 440, 699, 1199, 1685, 1856, 2092
Zsp2I ATGCAT 3 cut(s) 475, 1071, 2078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.