MD12G1008600.v1.1

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012209
Physical Location & Seq
Forward (+)
927675 .. 932133
4459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1008600.v1.1.491

Sequence Viewer

Length: 2247 bp
ATGGCCACCCAAACGTGGCAGCTCCATGAAGAAACACATCTCAGCCTCTGCCGCTCCTTCAACTTCTCCTTTCTCCTTCATCCCTCCTTATTAAAAACCAAGCAATCTTCTGCTCTAATTTCTCTGCTGTTCTGCAACAATACAACAATGTCCACAATGAGGTTGAAGCATCTCTCCTCCATTGCAAACGACGTTGTACAAAAATGCGCTTTGAAACTGGATACCCCGGTGGACAAATTGGTGGAGGAATTTGAAACTATATGGAAGACGGATGAAACGGCGCCGGTTGGTGCTGCTACTATCAGTAGTAACAACAACAACAATAGGAATAGTTATGGGAGGAAATTCGTAGAATTTTGCAGTGCCAAGGTTATGCAGAGTTGTGACATGTGCAAAACCATTGAGGAAAAGATTGCCAATGGCTCTTTTAGCCGCTTCACCTTCGACATGATGCTAGCTTGGGAGATGCCTACCTCTGCTGACGAGGAATCTTTTACGGAGTGTGTGGCGAAGGAGAAAGAAGAGAAAAAATTGCCGCAAAAGTGTCTTTCAGAACAAGATGACATCTCTCTGTTCTATTCAGATCTTATGCCGCTTCTTGTTGACAATGAGAAAAATGTTGGAGAAGATGCATTTGTTTGGATGGGGTCGCTGGTTCCACTAGTTACAGATGTTGTTAATGGGAGATTTACCTTTGAAACTTTAACGGTGCCTACGGGAAACCGGCTCCATTTTCCTTCCTATGACAAATTTTTAAAGGAAATTGATAGATGTATAAAATATTTGGCAAAACATGCAAAACCGAAGGGTGTGGAGCTTGCAGACGACGAATTCATTTTACACGTCGAGGGAACTGCTAGCACACAGCGAGTAGTGCGCCACATTGGAGGAACAAGTTGGCCTGGTAGGCTTACGCTAACGAATTACGCACTCTATTTCGAGGCTTCTGGAGTATTAACATATGAAGATGCTCTTAAAATAGACCTTTCGAAGGATATGGAGCAAACGGTGAAACCAGCTTCAACAGGGCCATGGGGTGCTCCACTTTTTGACAAGGCTATAATTTACGAGTCTCCTGAATTATCAGAGGAGATTGTCCTGGAGTTCCCCGAGGTAACAAGCTCTACAAGGCGTGACCTTTGGCTTGCGCTCTCAAAGGAGATTATGTTGATGCATCGTTTCGTAGCAAAGTATGAAATAAATTGTCCGATACAAGCATGGGAGATGCATTCAAGGACAATCCTAGCAATAACAAGGCTCCATGCAGCAAGAGAGATGCTAAGAATATCACCCCCTCCACCAACAAGATTCTTAATTTTTTCTTTGTTCGATGAGATACCGAAGGGAGATTACGTACTGGAAGAGCTTGCAGAGAGTCTTAAGAAGAGCAATAGCGGGCATCCATGTAGTGCTAGTTCAATCCTAAGGAGCATGAACTTGTCAGAGTCGATGATCATCTCAGGTTTAGACCAAGAGATGGAAGTTGGCAGCAGCGAAAATGCAACCCCAACTCCAAGCGGTCAAGTCGAAAACAGTTCCTTATTAGAAACAGCAATTAATCAGTCAAGAGAGGAAGAAAAGGATATTGCTATTGCCAAAGCTACCACAAAGGAACTTAAAGAAGAGGGAATCAGTGAAAGCGCTACTATCTTTTTGGAGCTACTAAGGCCCCTTAGGAATTCAGTGCCTTGGTTTGAAGAAGTACTCATGTGGGAAAGACCGTCAACTACTCTCACTGTGGTTGCTGCGACTTTAGTAGTCACCTACCAGGAATGGGTTGGCATAGCACTTGCCGCTTTATTCATTTGGCTGGTTGCGAAGATGGTTAGAGCCAGGCTGCAAAGACTTGATATAAAATGCAATGAGATAGTCGTATGCACCGCCTCGGACCAGAGTACAATAGAAAGCATAGTGTCTGCTCAACGTGGAATGCAAACTGTGCAGGAGATGATGCAGATAGCAAATGTTTCGATATTGAAGTTGTGGTCCATATATATTTCGAAGGCACGCAAGCATGCAAATGTGGCGATGGTGTTGTTGAGTGCGACAGCAATATTTTTTACGGTGGTTCCGGTGAAGTTCGTTATAATGGCAGCCATAGTTTGTTGCTTCTCATCGACATTGGCATCGAGACTAGGTATGGGTAAGGGGGAGAACCAAAGCAGCAGACGACTAAGAGAATGGTGGGATTCAATTCCAGTCATTCCTGTCCGTGTTGTCGAGACTCGACAGTCCAAGGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

749

Amino Acids

84.13

Weight (kDa)

5.51

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 514 - 741 1.1e-64 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21720 AT2G21720
fragaria_vesca FvH4_1g28470 FvH4_1g28470 FvH4_1g28470
malus_domestica MD12G1008600.v1.1 MD14G1006000.v1.1
prunus_persica Prupe.7G010400_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0496361 RchiOBHm_Chr3g0496371
rosa_laevigata RLG00000022528
rosa_multiflora Rmu_sc0002461.1_g000006
rosa_roxburghii Rroxscaffold_6G00389250
rosa_rugosa Rorug03G0282000
rosa_samantha Rh3AG328600 Rh3BG364700 Rh3CG361700 Rh3DG364700
rosa_wichuraiana Rw3G028770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2087
AasI GACNNNNNNGTC 1 cut(s) 2230
AccB1I GGYRCC 2 cut(s) 280, 709
AccB7I CCANNNNNTGG 1 cut(s) 1477
AccBSI CCGCTC 1 cut(s) 54
AciI CCGC 8 cut(s) 52, 433, 536, 593, 1395, 1518, 1794, 1881
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 6 cut(s) 248, 344, 353, 749, 830, 1678
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 4 cut(s) 198, 1356, 1704, 1897
AfeI AGCGCT 1 cut(s) 1642
AfiI CCNNNNNNNGG 5 cut(s) 15, 159, 991, 1477, 1774
AflII CTTAAG 1 cut(s) 1379
AflIII ACRYGT 2 cut(s) 387, 841
AhlI ACTAGT 1 cut(s) 661
AjiI CACGTC 1 cut(s) 844
AjnI CCWGG 4 cut(s) 901, 1098, 1767, 1832
AluBI AGCT 8 cut(s) 22, 458, 817, 1019, 1122, 1366, 1601, 1660
AluI AGCT 8 cut(s) 22, 458, 817, 1019, 1122, 1366, 1601, 1660
Alw21I GWGCWC 1 cut(s) 1042
Alw26I GTCTC 3 cut(s) 1077, 2125, 2216
AlwNI CAGNNNCTG 1 cut(s) 48
Ama87I CYCGRG 1 cut(s) 1109
Aor51HI AGCGCT 1 cut(s) 1642
AoxI GGCC 4 cut(s) 3, 899, 1028, 1667
ApeKI GCWGC 9 cut(s) 19, 293, 1265, 1488, 1491, 1745, 1837, 2093, 2163
ApoI RAATTY 6 cut(s) 248, 344, 353, 749, 830, 1678
AseI ATTAAT 1 cut(s) 1557
AspLEI GCGC 5 cut(s) 209, 283, 879, 1150, 1643
AspS9I GGNCC 4 cut(s) 1028, 1668, 1888, 1986
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 5 cut(s) 430, 1021, 1281, 1753, 2086
AsuII TTCGAA 2 cut(s) 989, 2000
AsuNHI GCTAGC 2 cut(s) 454, 857
AvaI CYCGRG 1 cut(s) 1109
AvaII GGWCC 2 cut(s) 1888, 1986
AxyI CCTNAGG 2 cut(s) 1424, 1673
BaeI ACNNNNGTAYC 2 cut(s) 213, 246
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 280, 709
BarI GAAGNNNNNNTAC 2 cut(s) 1334, 1366
BbsI GAAGAC 1 cut(s) 272
Bbv12I GWGCWC 1 cut(s) 1042
BbvI GCAGC 9 cut(s) 31, 280, 1277, 1500, 1503, 1732, 1824, 2105, 2175
BccI CCATC 4 cut(s) 637, 1471, 1816, 2023
BceAI ACGGC 1 cut(s) 294
BcgI CGANNNNNNTGC 4 cut(s) 836, 870, 1950, 1984
BciT130I CCWGG 4 cut(s) 903, 1100, 1769, 1834
BciVI GTATCC 1 cut(s) 214
BclI TGATCA 1 cut(s) 1452
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 3 cut(s) 1077, 2125, 2216
BcuI ACTAGT 1 cut(s) 661
BfaI CTAG 7 cut(s) 455, 662, 858, 1244, 1413, 2135, 2245
BfoI RGCGCY 2 cut(s) 284, 1644
BfrI CTTAAG 1 cut(s) 1379
BfuI GTATCC 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 907
BglII AGATCT 1 cut(s) 583
BmcAI AGTACT 1 cut(s) 1704
Bme1390I CCNGG 5 cut(s) 227, 903, 1100, 1769, 1834
Bme18I GGWCC 2 cut(s) 1888, 1986
BmeT110I CYCGRG 1 cut(s) 1109
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 4 cut(s) 1028, 1668, 1888, 1986
BmiI GGNNCC 7 cut(s) 282, 657, 711, 728, 1259, 1670, 2070
BmrFI CCNGG 5 cut(s) 227, 903, 1100, 1769, 1834
BmtI GCTAGC 2 cut(s) 458, 861
BpiI GAAGAC 1 cut(s) 272
BpmI CTGGAG 2 cut(s) 969, 1121
Bpu14I TTCGAA 2 cut(s) 989, 2000
BpuMI CCSGG 1 cut(s) 227
BsaAI YACGTR 1 cut(s) 1354
BsaBI GATNNNNATC 1 cut(s) 1454
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 7 cut(s) 225, 366, 1031, 1110, 1688, 1884, 2235
BsaWI WCCGGW 1 cut(s) 2071
BsaXI ACNNNNNCTCC 6 cut(s) 1151, 1181, 1338, 1368, 1495, 1525
Bsc4I CCNNNNNNNGG 5 cut(s) 15, 159, 991, 1477, 1774
Bse118I RCCGGY 2 cut(s) 283, 723
Bse1I ACTGG 3 cut(s) 222, 1362, 2198
Bse21I CCTNAGG 2 cut(s) 1424, 1673
Bse3DI GCAATG 2 cut(s) 180, 1867
Bse8I GATNNNNATC 1 cut(s) 1454
BseBI CCWGG 4 cut(s) 903, 1100, 1769, 1834
BseDI CCNNGG 7 cut(s) 225, 366, 1031, 1110, 1688, 1884, 2235
BseGI GGATG 4 cut(s) 79, 277, 648, 1399
BseJI GATNNNNATC 1 cut(s) 1454
BseLI CCNNNNNNNGG 5 cut(s) 15, 159, 991, 1477, 1774
BseMI GCAATG 2 cut(s) 180, 1867
BseMII CTCAG 2 cut(s) 55, 1473
BseNI ACTGG 3 cut(s) 222, 1362, 2198
BseRI GAGGAG 2 cut(s) 166, 1103
BseXI GCAGC 9 cut(s) 31, 280, 1277, 1500, 1503, 1732, 1824, 2105, 2175
BsgI GTGCAG 1 cut(s) 1961
BshFI GGCC 4 cut(s) 5, 901, 1030, 1669
BshNI GGYRCC 2 cut(s) 280, 709
BsiHKAI GWGCWC 1 cut(s) 1042
BsiHKCI CYCGRG 1 cut(s) 1109
BsiSI CCGG 4 cut(s) 227, 284, 724, 2072
BslI CCNNNNNNNGG 5 cut(s) 15, 159, 991, 1477, 1774
BsmAI GTCTC 3 cut(s) 1077, 2125, 2216
BsmI GAATGC 2 cut(s) 1228, 1935
BsnI GGCC 4 cut(s) 5, 901, 1030, 1669
BsoBI CYCGRG 1 cut(s) 1109
Bsp119I TTCGAA 2 cut(s) 989, 2000
Bsp1286I GDGCHC 1 cut(s) 1042
Bsp1407I TGTACA 1 cut(s) 196
Bsp143I GATC 2 cut(s) 583, 1452
Bsp19I CCATGG 1 cut(s) 1031
BspACI CCGC 8 cut(s) 52, 433, 536, 593, 1395, 1518, 1794, 1881
BspANI GGCC 4 cut(s) 5, 901, 1030, 1669
BspCNI CTCAG 2 cut(s) 54, 1472
BspLI GGNNCC 7 cut(s) 282, 657, 711, 728, 1259, 1670, 2070
BspOI GCTAGC 2 cut(s) 458, 861
BspQI GCTCTTC 2 cut(s) 1356, 1379
BspT104I TTCGAA 2 cut(s) 989, 2000
BspT107I GGYRCC 2 cut(s) 280, 709
BspTI CTTAAG 1 cut(s) 1379
BsrBI CCGCTC 1 cut(s) 54
BsrDI GCAATG 2 cut(s) 180, 1867
BsrFI RCCGGY 2 cut(s) 283, 723
BsrGI TGTACA 1 cut(s) 196
BsrI ACTGG 3 cut(s) 222, 1362, 2198
BssAI RCCGGY 2 cut(s) 283, 723
BssECI CCNNGG 7 cut(s) 225, 366, 1031, 1110, 1688, 1884, 2235
BssMI GATC 2 cut(s) 583, 1452
BssNI GRCGYC 1 cut(s) 281
BssT1I CCWWGG 4 cut(s) 366, 1031, 1688, 2235
Bst2UI CCWGG 4 cut(s) 903, 1100, 1769, 1834
Bst4CI ACNGT 8 cut(s) 709, 1009, 1535, 1722, 1738, 1939, 2065, 2232
Bst6I CTCTTC 4 cut(s) 516, 1356, 1379, 1617
BstACI GRCGYC 1 cut(s) 281
BstAFI CTTAAG 1 cut(s) 1379
BstAPI GCANNNNNTGC 2 cut(s) 794, 1939
BstAUI TGTACA 1 cut(s) 196
BstBAI YACGTR 1 cut(s) 1354
BstBI TTCGAA 2 cut(s) 989, 2000
BstC8I GCNNGC 9 cut(s) 456, 819, 859, 1146, 1368, 1397, 2008, 2012, 2016
BstDEI CTNAG 7 cut(s) 41, 1280, 1424, 1459, 1664, 1673, 2174
BstDSI CCRYGG 1 cut(s) 1031
BstENI CCTNNNNNAGG 1 cut(s) 989
BstF5I GGATG 4 cut(s) 79, 277, 648, 1399
BstH2I RGCGCY 2 cut(s) 284, 1644
BstHHI GCGC 5 cut(s) 209, 283, 879, 1150, 1643
BstKTI GATC 2 cut(s) 586, 1455
BstMAI GTCTC 3 cut(s) 1077, 2125, 2216
BstMBI GATC 2 cut(s) 583, 1452
BstMWI GCNNNNNNNGC 9 cut(s) 51, 429, 794, 874, 907, 1666, 1793, 1939, 2024
BstNI CCWGG 4 cut(s) 903, 1100, 1769, 1834
BstNSI RCATGY 3 cut(s) 391, 797, 2018
BstSCI CCNGG 5 cut(s) 225, 901, 1098, 1767, 1832
BstSNI TACGTA 1 cut(s) 1354
BstV1I GCAGC 9 cut(s) 31, 280, 1277, 1500, 1503, 1732, 1824, 2105, 2175
BstV2I GAAGAC 1 cut(s) 272
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
Bsu36I CCTNAGG 2 cut(s) 1424, 1673
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 4 cut(s) 5, 901, 1030, 1669
BtgI CCRYGG 1 cut(s) 1031
BtgZI GCGATG 1 cut(s) 2042
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 4 cut(s) 79, 277, 648, 1399
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 4 cut(s) 367, 1639, 1689, 1734
Cac8I GCNNGC 9 cut(s) 456, 819, 859, 1146, 1368, 1397, 2008, 2012, 2016
CaiI CAGNNNCTG 1 cut(s) 48
CfoI GCGC 5 cut(s) 209, 283, 879, 1150, 1643
Cfr10I RCCGGY 2 cut(s) 283, 723
Cfr13I GGNCC 4 cut(s) 1028, 1668, 1888, 1986
Csp6I GTAC 4 cut(s) 197, 1355, 1703, 1896
CviQI GTAC 4 cut(s) 197, 1355, 1703, 1896
DdeI CTNAG 7 cut(s) 41, 1280, 1424, 1459, 1664, 1673, 2174
DinI GGCGCC 1 cut(s) 282
DpnI GATC 2 cut(s) 585, 1454
DpnII GATC 2 cut(s) 583, 1452
DraI TTTAAA 1 cut(s) 756
DrdI GACNNNNNNGTC 1 cut(s) 2230
DseDI GACNNNNNNGTC 1 cut(s) 2230
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 516, 1356, 1379, 1617
EarI CTCTTC 4 cut(s) 516, 1356, 1379, 1617
Eco105I TACGTA 1 cut(s) 1354
Eco130I CCWWGG 4 cut(s) 366, 1031, 1688, 2235
Eco47I GGWCC 2 cut(s) 1888, 1986
Eco47III AGCGCT 1 cut(s) 1642
Eco81I CCTNAGG 2 cut(s) 1424, 1673
Eco88I CYCGRG 1 cut(s) 1109
EcoNI CCTNNNNNAGG 1 cut(s) 989
EcoO109I RGGNCCY 1 cut(s) 1668
EcoRI GAATTC 2 cut(s) 830, 1678
EcoRII CCWGG 4 cut(s) 901, 1098, 1767, 1832
EcoT14I CCWWGG 4 cut(s) 366, 1031, 1688, 2235
EcoT22I ATGCAT 3 cut(s) 634, 1176, 1230
EgeI GGCGCC 1 cut(s) 282
EheI GGCGCC 1 cut(s) 282
ErhI CCWWGG 4 cut(s) 366, 1031, 1688, 2235
FalI AAGNNNNNCTT 2 cut(s) 957, 989
FauI CCCGC 1 cut(s) 1388
FauNDI CATATG 1 cut(s) 961
FbaI TGATCA 1 cut(s) 1452
FokI GGATG 4 cut(s) 66, 284, 655, 1386
FspBI CTAG 7 cut(s) 455, 662, 858, 1244, 1413, 2135, 2245
GlaI GCGC 5 cut(s) 208, 282, 878, 1149, 1642
GsuI CTGGAG 2 cut(s) 969, 1121
HaeII RGCGCY 2 cut(s) 284, 1644
HaeIII GGCC 4 cut(s) 5, 901, 1030, 1669
HapII CCGG 4 cut(s) 227, 284, 724, 2072
HhaI GCGC 5 cut(s) 209, 283, 879, 1150, 1643
Hin1I GRCGYC 1 cut(s) 281
Hin6I GCGC 5 cut(s) 207, 281, 877, 1148, 1641
HinP1I GCGC 5 cut(s) 207, 281, 877, 1148, 1641
HincII GTYRAC 2 cut(s) 604, 1725
HindII GTYRAC 2 cut(s) 604, 1725
HinfI GANTC 8 cut(s) 488, 1070, 1308, 1375, 1445, 1629, 2189, 2224
HpaII CCGG 4 cut(s) 227, 284, 724, 2072
HphI GGTGA 5 cut(s) 430, 1021, 1281, 1753, 2086
Hpy166II GTNNAC 4 cut(s) 153, 232, 604, 1725
Hpy188I TCNGA 6 cut(s) 553, 583, 1087, 1209, 1444, 1888
Hpy188III TCNNGA 5 cut(s) 948, 1076, 1566, 2130, 2221
Hpy8I GTNNAC 4 cut(s) 153, 232, 604, 1725
Hpy99I CGWCG 3 cut(s) 194, 830, 848
HpyAV CCTTC 9 cut(s) 67, 86, 451, 505, 747, 799, 985, 1336, 1996
HpyCH4III ACNGT 8 cut(s) 709, 1009, 1535, 1722, 1738, 1939, 2065, 2232
HpyCH4IV ACGT 5 cut(s) 14, 192, 843, 1353, 1924
HpyF10VI GCNNNNNNNGC 9 cut(s) 51, 429, 794, 874, 907, 1666, 1793, 1939, 2024
HpyF3I CTNAG 7 cut(s) 41, 1280, 1424, 1459, 1664, 1673, 2174
HpySE526I ACGT 5 cut(s) 14, 192, 843, 1353, 1924
Hsp92I GRCGYC 1 cut(s) 281
HspAI GCGC 5 cut(s) 207, 281, 877, 1148, 1641
KasI GGCGCC 1 cut(s) 280
Ksp22I TGATCA 1 cut(s) 1452
Kzo9I GATC 2 cut(s) 583, 1452
LguI GCTCTTC 2 cut(s) 1356, 1379
LmnI GCTCC 9 cut(s) 27, 59, 732, 814, 1000, 1045, 1263, 1428, 1657
Lsp1109I GCAGC 9 cut(s) 31, 280, 1277, 1500, 1503, 1732, 1824, 2105, 2175
MaeI CTAG 7 cut(s) 455, 662, 858, 1244, 1413, 2135, 2245
MaeII ACGT 5 cut(s) 14, 192, 843, 1353, 1924
MaeIII GTNAC 6 cut(s) 308, 383, 664, 1114, 1133, 1759
MalI GATC 2 cut(s) 585, 1454
MbiI CCGCTC 1 cut(s) 54
MboI GATC 2 cut(s) 583, 1452
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 1 cut(s) 1042
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 281
MlyI GAGTC 4 cut(s) 1079, 1384, 1454, 2218
MmeI TCCRAC 1 cut(s) 601
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 3 cut(s) 634, 1176, 1230
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 2019
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 1379
MspI CCGG 4 cut(s) 227, 284, 724, 2072
MspR9I CCNGG 5 cut(s) 227, 903, 1100, 1769, 1834
Mva1269I GAATGC 2 cut(s) 1228, 1935
MvaI CCWGG 4 cut(s) 903, 1100, 1769, 1834
MwoI GCNNNNNNNGC 9 cut(s) 51, 429, 794, 874, 907, 1666, 1793, 1939, 2024
NarI GGCGCC 1 cut(s) 281
NciI CCSGG 1 cut(s) 227
NcoI CCATGG 1 cut(s) 1031
NdeI CATATG 1 cut(s) 961
NdeII GATC 2 cut(s) 583, 1452
NheI GCTAGC 2 cut(s) 454, 857
NlaIV GGNNCC 7 cut(s) 282, 657, 711, 728, 1259, 1670, 2070
NmuCI GTSAC 3 cut(s) 383, 1133, 1759
NsiI ATGCAT 3 cut(s) 634, 1176, 1230
NspI RCATGY 3 cut(s) 391, 797, 2018
NspV TTCGAA 2 cut(s) 989, 2000
PaeI GCATGC 1 cut(s) 2018
PciI ACATGT 1 cut(s) 387
PciSI GCTCTTC 2 cut(s) 1356, 1379
PctI GAATGC 2 cut(s) 1228, 1935
PfeI GAWTC 4 cut(s) 488, 1308, 1629, 2189
PflMI CCANNNNNTGG 1 cut(s) 1477
PfoI TCCNGGA 1 cut(s) 1098
PleI GAGTC 4 cut(s) 1078, 1383, 1453, 2218
PluTI GGCGCC 1 cut(s) 284
PpsI GAGTC 4 cut(s) 1078, 1383, 1453, 2218
Ppu21I YACGTR 1 cut(s) 1354
PscI ACATGT 1 cut(s) 387
PshBI ATTAAT 1 cut(s) 1557
PsiI TTATAA 1 cut(s) 2087
Psp6I CCWGG 4 cut(s) 901, 1098, 1767, 1832
PspGI CCWGG 4 cut(s) 901, 1098, 1767, 1832
PspN4I GGNNCC 7 cut(s) 282, 657, 711, 728, 1259, 1670, 2070
PspPI GGNCC 4 cut(s) 1028, 1668, 1888, 1986
PsrI GAACNNNNNNTAC 2 cut(s) 1399, 1431
PstNI CAGNNNCTG 1 cut(s) 48
PsuI RGATCY 1 cut(s) 583
RsaI GTAC 4 cut(s) 198, 1356, 1704, 1897
RsaNI GTAC 4 cut(s) 197, 1355, 1703, 1896
RseI CAYNNNNRTG 1 cut(s) 2019
SapI GCTCTTC 2 cut(s) 1356, 1379
Sau3AI GATC 2 cut(s) 583, 1452
Sau96I GGNCC 4 cut(s) 1028, 1668, 1888, 1986
ScaI AGTACT 1 cut(s) 1704
SchI GAGTC 4 cut(s) 1079, 1384, 1454, 2218
ScrFI CCNGG 5 cut(s) 227, 903, 1100, 1769, 1834
SduI GDGCHC 1 cut(s) 1042
SfoI GGCGCC 1 cut(s) 282
SfuI TTCGAA 2 cut(s) 989, 2000
SinI GGWCC 2 cut(s) 1888, 1986
SmiMI CAYNNNNRTG 1 cut(s) 2019
SmlI CTYRAG 1 cut(s) 1379
SmoI CTYRAG 1 cut(s) 1379
SnaBI TACGTA 1 cut(s) 1354
SpeI ACTAGT 1 cut(s) 661
SphI GCATGC 1 cut(s) 2018
SsiI CCGC 8 cut(s) 52, 433, 536, 593, 1395, 1518, 1794, 1881
SspDI GGCGCC 1 cut(s) 280
SspI AATATT 2 cut(s) 782, 2055
SspMI CTAG 7 cut(s) 455, 662, 858, 1244, 1413, 2135, 2245
StyD4I CCNGG 5 cut(s) 225, 901, 1098, 1767, 1832
StyI CCWWGG 4 cut(s) 366, 1031, 1688, 2235
TaaI ACNGT 8 cut(s) 709, 1009, 1535, 1722, 1738, 1939, 2065, 2232
TaiI ACGT 5 cut(s) 17, 195, 846, 1356, 1927
TatI WGTACW 3 cut(s) 196, 1702, 1895
TauI GCSGC 5 cut(s) 54, 435, 538, 595, 1796
TfiI GAWTC 4 cut(s) 488, 1308, 1629, 2189
TscAI CASTG 4 cut(s) 367, 1639, 1689, 1741
TseFI GTSAC 3 cut(s) 383, 1133, 1759
TseI GCWGC 9 cut(s) 19, 293, 1265, 1488, 1491, 1745, 1837, 2093, 2163
Tsp45I GTSAC 3 cut(s) 383, 1133, 1759
TspDTI ATGAA 8 cut(s) 42, 68, 288, 823, 978, 1209, 1448, 1792
TspGWI ACGGA 3 cut(s) 284, 512, 2201
TspRI CASTG 4 cut(s) 367, 1639, 1689, 1741
Van91I CCANNNNNTGG 1 cut(s) 1477
Vha464I CTTAAG 1 cut(s) 1379
VpaK11BI GGWCC 2 cut(s) 1888, 1986
VspI ATTAAT 1 cut(s) 1557
XagI CCTNNNNNAGG 1 cut(s) 989
XapI RAATTY 6 cut(s) 248, 344, 353, 749, 830, 1678
XceI RCATGY 3 cut(s) 391, 797, 2018
XcmI CCANNNNNNNNNTGG 1 cut(s) 1775
XspI CTAG 7 cut(s) 455, 662, 858, 1244, 1413, 2135, 2245
ZrmI AGTACT 1 cut(s) 1704
Zsp2I ATGCAT 3 cut(s) 634, 1176, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.