AT2G24220

Purine permease 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012527
Physical Location & Seq
Forward (+)
10300032 .. 10301787
1756 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G24220.1

Sequence Viewer

Length: 1086 bp
ATGGATGAAACAACAGAACGCCCAACTGTTTCGTTTTCATATTCAAATTGGATATCAAACATCAAAAAATCAACACGAGAAGCTTACGAAGCAAAGCCCTTTTCACATTGGATTCTTTTGTTCTTCAGTGGTGCGGCAATGCTCATAGCTTTCCCAGCTTCTAGTCTACTCTCTCGTCTTTACTTCTCCAATGGTGGTAAAAGCAAATGGATCATTTCTTGGGTTGCTGTTGCTGGATGGCCAATCACTTGTCTTATTTTACTTCCTACATATATCTTTCAAAAGATCAAACCAACACCTCTCAATACAAAGCTTGTTCTCTCTTATGTTGTGCTCGGTTTCTTGAGTGCTGCAGACAACCTTATGTATGCTTATGCCTATGCGTACCTCCCTGCTTCGACTTCTTCTCTTCTGGCTTCTTCGTCGCTTGCCTTCTCTGCTTTGTTTGGCTATCTCATTGTTAAAAACCCGCTGAATGCTTCGGTTATCAACTCCATCGTGGTTATAACCGGTGCTATGGCTATAATAGCGCTGGATTCAAGCTCTGATCGGTACAGTTATATAAGCAACAGTCAATACTTTGCAGGTTTCTTTTGGGACATAATGGGATCTGCGCTTCACGGGCTCATTTTCGCGTTATCTGAGCTTCTCTTTGTGAAATTGCTTGGAAGAAGGTCTTTTCACGTTGCTTTGGAGCAGCAAGTCATGGTTTCTCTCACAGCTTTTGCGTTTACCACTATTGGGATGGTTGTGAGCAATGATTTCCAAGGGATGAGTCACGAAGCTAAGAGTTTCAAAGGCGGTGAGTCTCTGTACACTCAGGTTCTTGTCTGGAGTGCAGTCACGTTTCAACTGGGTGTTCTTGGGGCGACCGCGGTCTTGTTTCTGGCATCCACAGTCATGGCTGGAGTCCTTAACGCTGTGAGAGTTCCCATCACGAGCGTAGCCGCTGTTATATTGATGCATGATCCAATGAGTGGCTTCAAAATCCTGTCTTTGGTTTTGACGTTCTGGGGATTCAGCTCTTATATCTACGGGAGCTCGAGCTCGAACTCAAGTACTCAAGCTTCCTCTTCTTCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

361

Amino Acids

39.17

Weight (kDa)

9.13

Isoelectric Point (pI)

32.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 38 - 352 5.7e-105 Purine nucleobase transmembrane transport
EamA PF00892 69 - 177 7.2e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 506
Acc36I ACCTGC 1 cut(s) 575
AccB7I CCANNNNNTGG 1 cut(s) 977
AccI GTMKAC 1 cut(s) 166
AccII CGCG 2 cut(s) 635, 875
AciI CCGC 6 cut(s) 134, 470, 801, 873, 875, 948
AclWI GGATC 3 cut(s) 218, 616, 962
AcoI YGGCCR 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 109
AfaI GTAC 4 cut(s) 386, 554, 815, 1060
AfeI AGCGCT 1 cut(s) 531
AfiI CCNNNNNNNGG 3 cut(s) 741, 977, 997
AgeI ACCGGT 1 cut(s) 509
AgsI TTSAA 6 cut(s) 45, 281, 540, 796, 851, 985
Alw21I GWGCWC 3 cut(s) 336, 1043, 1049
Alw26I GTCTC 1 cut(s) 813
AlwI GGATC 3 cut(s) 218, 616, 962
Ama87I CYCGRG 1 cut(s) 1042
Aor51HI AGCGCT 1 cut(s) 531
AoxI GGCC 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 350, 697
AsiGI ACCGGT 1 cut(s) 509
AspLEI GCGC 2 cut(s) 532, 616
AsuHPI GGTGA 1 cut(s) 815
AvaI CYCGRG 1 cut(s) 1042
BalI TGGCCA 1 cut(s) 241
BanII GRGCYC 3 cut(s) 627, 1043, 1049
BarI GAAGNNNNNNTAC 2 cut(s) 1051, 1083
BauI CACGAG 2 cut(s) 75, 937
Bbv12I GWGCWC 3 cut(s) 336, 1043, 1049
BbvI GCAGC 2 cut(s) 337, 709
BccI CCATC 4 cut(s) 231, 503, 739, 941
BcoDI GTCTC 1 cut(s) 813
BfaI CTAG 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 351
BfoI RGCGCY 1 cut(s) 533
BfuAI ACCTGC 1 cut(s) 575
BisI GCNGC 4 cut(s) 135, 351, 698, 948
BlsI GCNGC 4 cut(s) 136, 352, 699, 949
BmcAI AGTACT 1 cut(s) 1060
BmeT110I CYCGRG 1 cut(s) 1042
BmrI ACTGGG 1 cut(s) 863
BmsI GCATC 2 cut(s) 899, 951
BmuI ACTGGG 1 cut(s) 863
BoxI GACNNNNGTC 1 cut(s) 875
BpmI CTGGAG 2 cut(s) 853, 927
BpuEI CTTGAG 3 cut(s) 364, 1039, 1047
BsaJI CCNNGG 2 cut(s) 766, 873
BsaWI WCCGGW 1 cut(s) 509
Bsc4I CCNNNNNNNGG 3 cut(s) 741, 977, 997
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 1 cut(s) 858
Bse3DI GCAATG 2 cut(s) 144, 763
BseDI CCNNGG 2 cut(s) 766, 873
BseGI GGATG 6 cut(s) 10, 242, 750, 777, 890, 1079
BseLI CCNNNNNNNGG 3 cut(s) 741, 977, 997
BseMI GCAATG 2 cut(s) 144, 763
BseMII CTCAG 2 cut(s) 633, 833
BseNI ACTGG 1 cut(s) 858
BseXI GCAGC 2 cut(s) 337, 709
BseYI CCCAGC 1 cut(s) 154
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 2 cut(s) 635, 875
Bsh1285I CGRYCG 1 cut(s) 873
BshFI GGCC 1 cut(s) 241
BshTI ACCGGT 1 cut(s) 509
BsiEI CGRYCG 1 cut(s) 873
BsiHKAI GWGCWC 3 cut(s) 336, 1043, 1049
BsiHKCI CYCGRG 1 cut(s) 1042
BsiSI CCGG 1 cut(s) 510
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 3 cut(s) 741, 977, 997
BsmAI GTCTC 1 cut(s) 813
BsmFI GGGAC 1 cut(s) 611
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 1 cut(s) 241
BsoBI CYCGRG 1 cut(s) 1042
Bsp1286I GDGCHC 4 cut(s) 336, 627, 1043, 1049
Bsp1407I TGTACA 1 cut(s) 813
Bsp143I GATC 5 cut(s) 210, 285, 547, 608, 967
BspACI CCGC 6 cut(s) 134, 470, 801, 873, 875, 948
BspANI GGCC 1 cut(s) 241
BspCNI CTCAG 2 cut(s) 634, 832
BspFNI CGCG 2 cut(s) 635, 875
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 575
BspPI GGATC 3 cut(s) 218, 616, 962
BsrDI GCAATG 2 cut(s) 144, 763
BsrFI RCCGGY 1 cut(s) 509
BsrGI TGTACA 1 cut(s) 813
BsrI ACTGG 1 cut(s) 858
BssAI RCCGGY 1 cut(s) 509
BssECI CCNNGG 2 cut(s) 766, 873
BssMI GATC 5 cut(s) 210, 285, 547, 608, 967
BssSI CACGAG 2 cut(s) 75, 937
BssT1I CCWWGG 1 cut(s) 766
Bst2BI CACGAG 2 cut(s) 75, 937
Bst4CI ACNGT 4 cut(s) 28, 557, 572, 898
Bst6I CTCTTC 2 cut(s) 414, 1078
BstAUI TGTACA 1 cut(s) 813
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 3 cut(s) 642, 786, 819
BstDSI CCRYGG 1 cut(s) 873
BstF5I GGATG 6 cut(s) 10, 242, 750, 777, 890, 1079
BstFNI CGCG 2 cut(s) 635, 875
BstH2I RGCGCY 1 cut(s) 533
BstHHI GCGC 2 cut(s) 532, 616
BstKTI GATC 5 cut(s) 213, 288, 550, 611, 970
BstMAI GTCTC 1 cut(s) 813
BstMBI GATC 5 cut(s) 210, 285, 547, 608, 967
BstMCI CGRYCG 1 cut(s) 873
BstMWI GCNNNNNNNGC 5 cut(s) 89, 155, 437, 527, 622
BstPAI GACNNNNGTC 1 cut(s) 875
BstSFI CTRYAG 1 cut(s) 351
BstUI CGCG 2 cut(s) 635, 875
BstV1I GCAGC 2 cut(s) 337, 709
BstX2I RGATCY 1 cut(s) 608
BstXI CCANNNNNNTGG 1 cut(s) 901
BstYI RGATCY 1 cut(s) 608
BsuRI GGCC 1 cut(s) 241
BtgI CCRYGG 1 cut(s) 873
BtsCI GGATG 6 cut(s) 10, 242, 750, 777, 890, 1079
BtsIMutI CAGTG 1 cut(s) 133
BveI ACCTGC 1 cut(s) 575
Cac8I GCNNGC 1 cut(s) 429
CfoI GCGC 2 cut(s) 532, 616
Cfr10I RCCGGY 1 cut(s) 509
Cfr42I CCGCGG 1 cut(s) 876
Csp6I GTAC 4 cut(s) 385, 553, 814, 1059
CspAI ACCGGT 1 cut(s) 509
CviAII CATG 3 cut(s) 706, 901, 965
CviQI GTAC 4 cut(s) 385, 553, 814, 1059
DdeI CTNAG 3 cut(s) 642, 786, 819
DpnI GATC 5 cut(s) 212, 287, 549, 610, 969
DpnII GATC 5 cut(s) 210, 285, 547, 608, 967
EaeI YGGCCR 1 cut(s) 239
Eam1104I CTCTTC 2 cut(s) 414, 1078
EarI CTCTTC 2 cut(s) 414, 1078
Ecl136II GAGCTC 2 cut(s) 1041, 1047
Eco130I CCWWGG 1 cut(s) 766
Eco24I GRGCYC 3 cut(s) 627, 1043, 1049
Eco32I GATATC 1 cut(s) 54
Eco47III AGCGCT 1 cut(s) 531
Eco53kI GAGCTC 2 cut(s) 1041, 1047
Eco57I CTGAAG 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 1042
EcoICRI GAGCTC 2 cut(s) 1041, 1047
EcoRV GATATC 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 766
EcoT22I ATGCAT 1 cut(s) 966
EcoT38I GRGCYC 3 cut(s) 627, 1043, 1049
ErhI CCWWGG 1 cut(s) 766
FaeI CATG 3 cut(s) 709, 904, 968
FalI AAGNNNNNCTT 2 cut(s) 661, 693
FaqI GGGAC 1 cut(s) 611
FatI CATG 3 cut(s) 705, 900, 964
FauI CCCGC 1 cut(s) 477
FblI GTMKAC 1 cut(s) 166
Fnu4HI GCNGC 4 cut(s) 135, 351, 698, 948
FokI GGATG 6 cut(s) 17, 249, 757, 784, 877, 1066
FriOI GRGCYC 3 cut(s) 627, 1043, 1049
Fsp4HI GCNGC 4 cut(s) 135, 351, 698, 948
FspBI CTAG 1 cut(s) 162
GlaI GCGC 2 cut(s) 531, 615
GluI GCNGC 4 cut(s) 135, 351, 698, 948
GsaI CCCAGC 1 cut(s) 158
GsuI CTGGAG 2 cut(s) 853, 927
HaeII RGCGCY 1 cut(s) 533
HaeIII GGCC 1 cut(s) 241
HapII CCGG 1 cut(s) 510
HhaI GCGC 2 cut(s) 532, 616
Hin1II CATG 3 cut(s) 709, 904, 968
Hin6I GCGC 2 cut(s) 530, 614
HinP1I GCGC 2 cut(s) 530, 614
HindIII AAGCTT 3 cut(s) 81, 311, 1065
HinfI GANTC 6 cut(s) 112, 536, 775, 806, 909, 1017
HpaII CCGG 1 cut(s) 510
HphI GGTGA 1 cut(s) 815
Hpy166II GTNNAC 3 cut(s) 167, 732, 816
Hpy188I TCNGA 2 cut(s) 547, 643
Hpy188III TCNNGA 4 cut(s) 343, 779, 832, 937
Hpy8I GTNNAC 3 cut(s) 167, 732, 816
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 2 cut(s) 442, 666
HpyCH4III ACNGT 4 cut(s) 28, 557, 572, 898
HpyCH4IV ACGT 3 cut(s) 684, 845, 1007
HpyCH4V TGCA 4 cut(s) 353, 584, 839, 964
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 155, 437, 527, 622
HpyF3I CTNAG 3 cut(s) 642, 786, 819
HpySE526I ACGT 3 cut(s) 684, 845, 1007
Hsp92II CATG 3 cut(s) 709, 904, 968
HspAI GCGC 2 cut(s) 530, 614
KspI CCGCGG 1 cut(s) 876
Kzo9I GATC 5 cut(s) 210, 285, 547, 608, 967
LmnI GCTCC 2 cut(s) 694, 1038
Lsp1109I GCAGC 2 cut(s) 337, 709
LweI GCATC 2 cut(s) 899, 951
MaeI CTAG 1 cut(s) 162
MaeII ACGT 3 cut(s) 684, 845, 1007
MaeIII GTNAC 2 cut(s) 776, 841
MalI GATC 5 cut(s) 212, 287, 549, 610, 969
MboI GATC 5 cut(s) 210, 285, 547, 608, 967
MboII GAAGA 7 cut(s) 115, 396, 401, 411, 681, 1065, 1068
MflI RGATCY 1 cut(s) 608
MhlI GDGCHC 4 cut(s) 336, 627, 1043, 1049
MlsI TGGCCA 1 cut(s) 241
MluCI AATT 2 cut(s) 46, 659
MluNI TGGCCA 1 cut(s) 241
MlyI GAGTC 3 cut(s) 784, 815, 918
MnlI CCTC 3 cut(s) 309, 398, 1081
Mox20I TGGCCA 1 cut(s) 241
Mph1103I ATGCAT 1 cut(s) 966
MscI TGGCCA 1 cut(s) 241
MseI TTAA 2 cut(s) 462, 915
MslI CAYNNNNRTG 1 cut(s) 899
Msp20I TGGCCA 1 cut(s) 241
MspA1I CMGCKG 3 cut(s) 472, 875, 950
MspI CCGG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 481
MvnI CGCG 2 cut(s) 635, 875
MwoI GCNNNNNNNGC 5 cut(s) 89, 155, 437, 527, 622
NdeII GATC 5 cut(s) 210, 285, 547, 608, 967
NlaIII CATG 3 cut(s) 709, 904, 968
NmuCI GTSAC 2 cut(s) 776, 841
NsiI ATGCAT 1 cut(s) 966
PaeR7I CTCGAG 1 cut(s) 1042
PctI GAATGC 1 cut(s) 481
PfeI GAWTC 3 cut(s) 112, 536, 1017
PflMI CCANNNNNTGG 1 cut(s) 977
PinAI ACCGGT 1 cut(s) 509
PkrI GCNGC 4 cut(s) 136, 352, 699, 949
PleI GAGTC 3 cut(s) 783, 814, 917
PpsI GAGTC 3 cut(s) 783, 814, 917
PshAI GACNNNNGTC 1 cut(s) 875
PsiI TTATAA 1 cut(s) 506
Psp124BI GAGCTC 2 cut(s) 1043, 1049
PspFI CCCAGC 1 cut(s) 154
PspXI VCTCGAGB 1 cut(s) 1042
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 608
RsaI GTAC 4 cut(s) 386, 554, 815, 1060
RsaNI GTAC 4 cut(s) 385, 553, 814, 1059
RseI CAYNNNNRTG 1 cut(s) 899
SacI GAGCTC 2 cut(s) 1043, 1049
SacII CCGCGG 1 cut(s) 876
SaqAI TTAA 2 cut(s) 462, 915
SatI GCNGC 4 cut(s) 135, 351, 698, 948
Sau3AI GATC 5 cut(s) 210, 285, 547, 608, 967
ScaI AGTACT 1 cut(s) 1060
SchI GAGTC 3 cut(s) 784, 815, 918
SduI GDGCHC 4 cut(s) 336, 627, 1043, 1049
SfaNI GCATC 2 cut(s) 899, 951
SfcI CTRYAG 1 cut(s) 351
Sfr274I CTCGAG 1 cut(s) 1042
Sfr303I CCGCGG 1 cut(s) 876
SgrBI CCGCGG 1 cut(s) 876
SlaI CTCGAG 1 cut(s) 1042
SmiMI CAYNNNNRTG 1 cut(s) 899
SmlI CTYRAG 4 cut(s) 343, 1042, 1054, 1062
SmoI CTYRAG 4 cut(s) 343, 1042, 1054, 1062
Sse9I AATT 2 cut(s) 46, 659
SsiI CCGC 6 cut(s) 134, 470, 801, 873, 875, 948
SspMI CTAG 1 cut(s) 162
SstI GAGCTC 2 cut(s) 1043, 1049
StyI CCWWGG 1 cut(s) 766
TaaI ACNGT 4 cut(s) 28, 557, 572, 898
TaiI ACGT 3 cut(s) 687, 848, 1010
TaqI TCGA 3 cut(s) 398, 1043, 1049
TasI AATT 2 cut(s) 46, 659
TatI WGTACW 2 cut(s) 813, 1058
TauI GCSGC 2 cut(s) 137, 950
TfiI GAWTC 3 cut(s) 112, 536, 1017
Tru1I TTAA 2 cut(s) 462, 915
Tru9I TTAA 2 cut(s) 462, 915
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 2 cut(s) 776, 841
TseI GCWGC 2 cut(s) 350, 697
Tsp45I GTSAC 2 cut(s) 776, 841
TspDTI ATGAA 3 cut(s) 21, 27, 1068
TspRI CASTG 1 cut(s) 133
Van91I CCANNNNNTGG 1 cut(s) 977
XcmI CCANNNNNNNNNTGG 1 cut(s) 742
XhoI CTCGAG 1 cut(s) 1042
XmiI GTMKAC 1 cut(s) 166
XspI CTAG 1 cut(s) 162
ZrmI AGTACT 1 cut(s) 1060
Zsp2I ATGCAT 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.