pycom15g13680

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012527
Physical Location & Seq
Forward (+)
9279535 .. 9280470
936 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g13680.1

Sequence Viewer

Length: 936 bp
ATGCTTATAGCGTTTCCTGCATCGAGTCTTCTTTCTCGTGTGTATTATGCCAATGGAGGCACTAGCAAGTGGATCATTTCATGGGTTGCCGTAGCAGGGTGGCCTCTGACTGCTTTAATCTTATTGCCTACGTACATCGTCTGCAAAACGACCCCTACTCCCCTGAATATAAAACTTACTGTTTCTTATATTGTGCTAGGTCTACTAAGCGCTGCAGATAATCTCATGTATGCTTATGCATATGCTTACCTTCCAGCATCTACCGCTTCTCTTCTCGCATCATCATCTCTAGTATTTTCTGCATTATTCGGGCACCTACTTGTGAACAACAAACTTAATGCAGCGATAGTAAATGCTATTTTTATCATTACAGCTGCTATGACCATCATTGCTCTGGATTCAGATTCAGACAGATATGGAAATGTAACTGATAAGCAATACATTCTGGGATTTGTGTGGGATATTTTAGGATCAGCACTCCACGGGCTTATTTTCGCGCTTTCAGAGCGGGTTTTCGTGAAGCTACTTGGTAGAAGGTCCTTCCATGTTGTGTTGGAGCAGCAAGTCATGGTTTCTTTCTTTGCTTTCGTGTTCACCACTATCGGAGTAATCGTGAATAAGGACTTTCAGGGAATGAAAAACGAGGCCGGAATTTTCAAGGGCGGTAAGTCTGCTTATTATCTTGTTCTCATTTGGAGTGTCATCAGTTTCCAGTTAGGGGTATTAGGGGCTACTGCTGTGCTTTTCTTATCTTCCACCGTTCTCGCCGGTGTGCTGAACGCAGTAAGGGTTCCCCTCACCAGCATTGCAGCTGTCATATTGTTAAACGACCCGATGAGCGGGTTGAAGATCCTCTCCCTGATTGTTACATTTTGGGGCTTTGGCTGCTACATCTATGGCAGTTCCTCTGCATCTTCAAGTAGAAATTCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

312

Amino Acids

33.45

Weight (kDa)

9.35

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 1 - 304 3.2e-92 Purine nucleobase transmembrane transport
EamA PF00892 36 - 131 5.9e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 312
AccBSI CCGCTC 2 cut(s) 508, 840
AccI GTMKAC 1 cut(s) 202
AccII CGCG 1 cut(s) 497
AciI CCGC 4 cut(s) 264, 508, 663, 840
AclWI GGATC 3 cut(s) 80, 478, 844
AcsI RAATTY 2 cut(s) 651, 925
AfaI GTAC 1 cut(s) 134
AfeI AGCGCT 1 cut(s) 211
AfiI CCNNNNNNNGG 3 cut(s) 96, 718, 839
AgsI TTSAA 3 cut(s) 658, 847, 918
AluBI AGCT 3 cut(s) 374, 523, 812
AluI AGCT 3 cut(s) 374, 523, 812
AlwI GGATC 3 cut(s) 80, 478, 844
Aor51HI AGCGCT 1 cut(s) 211
AoxI GGCC 2 cut(s) 101, 645
ApeKI GCWGC 6 cut(s) 212, 341, 374, 559, 809, 885
ApoI RAATTY 2 cut(s) 651, 925
AspLEI GCGC 2 cut(s) 212, 499
AspS9I GGNCC 1 cut(s) 537
AsuHPI GGTGA 2 cut(s) 586, 790
AvaII GGWCC 1 cut(s) 537
BaeGI GKGCMC 1 cut(s) 315
BanI GGYRCC 1 cut(s) 312
BauI CACGAG 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 20
BbvI GCAGC 6 cut(s) 199, 353, 361, 571, 821, 872
BccI CCATC 1 cut(s) 392
BceAI ACGGC 1 cut(s) 74
BfaI CTAG 3 cut(s) 63, 197, 290
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 1 cut(s) 213
BisI GCNGC 6 cut(s) 213, 342, 375, 560, 810, 886
BlsI GCNGC 6 cut(s) 214, 343, 376, 561, 811, 887
Bme18I GGWCC 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 537
BmiI GGNNCC 2 cut(s) 314, 792
BmsI GCATC 4 cut(s) 29, 266, 287, 920
BpiI GAAGAC 1 cut(s) 20
BsaAI YACGTR 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 481
BsaXI ACNNNNNCTCC 4 cut(s) 142, 172, 597, 627
Bsc4I CCNNNNNNNGG 3 cut(s) 96, 718, 839
Bse118I RCCGGY 1 cut(s) 767
Bse1I ACTGG 1 cut(s) 712
Bse3DI GCAATG 2 cut(s) 387, 804
BseDI CCNNGG 1 cut(s) 481
BseLI CCNNNNNNNGG 3 cut(s) 96, 718, 839
BseMI GCAATG 2 cut(s) 387, 804
BseNI ACTGG 1 cut(s) 712
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 6 cut(s) 199, 353, 361, 571, 821, 872
Bsh1236I CGCG 1 cut(s) 497
BshFI GGCC 2 cut(s) 103, 647
BshNI GGYRCC 1 cut(s) 312
BsiSI CCGG 2 cut(s) 648, 768
BslI CCNNNNNNNGG 3 cut(s) 96, 718, 839
BsnI GGCC 2 cut(s) 103, 647
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 3 cut(s) 72, 470, 849
BspACI CCGC 4 cut(s) 264, 508, 663, 840
BspANI GGCC 2 cut(s) 103, 647
BspFNI CGCG 1 cut(s) 497
BspLI GGNNCC 2 cut(s) 314, 792
BspMAI CTGCAG 1 cut(s) 217
BspPI GGATC 3 cut(s) 80, 478, 844
BspT107I GGYRCC 1 cut(s) 312
BsrBI CCGCTC 2 cut(s) 508, 840
BsrDI GCAATG 2 cut(s) 387, 804
BsrFI RCCGGY 1 cut(s) 767
BsrI ACTGG 1 cut(s) 712
BssAI RCCGGY 1 cut(s) 767
BssECI CCNNGG 1 cut(s) 481
BssMI GATC 3 cut(s) 72, 470, 849
BssSI CACGAG 1 cut(s) 36
Bst2BI CACGAG 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 181, 760
Bst6I CTCTTC 1 cut(s) 276
BstBAI YACGTR 1 cut(s) 132
BstDEI CTNAG 1 cut(s) 206
BstDSI CCRYGG 1 cut(s) 481
BstFNI CGCG 1 cut(s) 497
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 2 cut(s) 212, 499
BstKTI GATC 3 cut(s) 75, 473, 852
BstMBI GATC 3 cut(s) 72, 470, 849
BstMWI GCNNNNNNNGC 4 cut(s) 17, 263, 505, 885
BstSFI CTRYAG 1 cut(s) 213
BstSLI GKGCMC 1 cut(s) 315
BstSNI TACGTA 1 cut(s) 132
BstUI CGCG 1 cut(s) 497
BstV1I GCAGC 6 cut(s) 199, 353, 361, 571, 821, 872
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 849
BstYI RGATCY 1 cut(s) 849
BsuRI GGCC 2 cut(s) 103, 647
BtgI CCRYGG 1 cut(s) 481
CfoI GCGC 2 cut(s) 212, 499
Cfr10I RCCGGY 1 cut(s) 767
Cfr13I GGNCC 1 cut(s) 537
Csp6I GTAC 1 cut(s) 133
CviAII CATG 4 cut(s) 81, 226, 545, 568
CviJI RGCY 9 cut(s) 103, 374, 487, 523, 647, 731, 812, 879, 885
CviKI_1 RGCY 9 cut(s) 103, 374, 487, 523, 647, 731, 812, 879, 885
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 1 cut(s) 206
DpnI GATC 3 cut(s) 74, 472, 851
DpnII GATC 3 cut(s) 72, 470, 849
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco105I TACGTA 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 537
Eco47III AGCGCT 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 537
EcoT22I ATGCAT 1 cut(s) 241
FaeI CATG 4 cut(s) 84, 229, 548, 571
FatI CATG 4 cut(s) 80, 225, 544, 567
FauI CCCGC 2 cut(s) 501, 833
FauNDI CATATG 1 cut(s) 241
FblI GTMKAC 1 cut(s) 202
Fnu4HI GCNGC 6 cut(s) 213, 342, 375, 560, 810, 886
Fsp4HI GCNGC 6 cut(s) 213, 342, 375, 560, 810, 886
FspBI CTAG 3 cut(s) 63, 197, 290
GlaI GCGC 2 cut(s) 211, 498
GluI GCNGC 6 cut(s) 213, 342, 375, 560, 810, 886
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 2 cut(s) 103, 647
HapII CCGG 2 cut(s) 648, 768
HhaI GCGC 2 cut(s) 212, 499
Hin1II CATG 4 cut(s) 84, 229, 548, 571
Hin6I GCGC 2 cut(s) 210, 497
HinP1I GCGC 2 cut(s) 210, 497
HinfI GANTC 3 cut(s) 25, 398, 404
HpaII CCGG 2 cut(s) 648, 768
HphI GGTGA 2 cut(s) 586, 790
Hpy166II GTNNAC 3 cut(s) 203, 325, 594
Hpy188I TCNGA 5 cut(s) 108, 403, 409, 505, 605
Hpy188III TCNNGA 4 cut(s) 395, 517, 613, 933
Hpy8I GTNNAC 3 cut(s) 203, 325, 594
HpyAV CCTTC 3 cut(s) 260, 528, 550
HpyCH4III ACNGT 2 cut(s) 181, 760
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 8 cut(s) 20, 144, 215, 239, 302, 341, 809, 911
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 263, 505, 885
HpyF3I CTNAG 1 cut(s) 206
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 4 cut(s) 84, 229, 548, 571
HspAI GCGC 2 cut(s) 210, 497
Kzo9I GATC 3 cut(s) 72, 470, 849
LmnI GCTCC 1 cut(s) 556
Lsp1109I GCAGC 6 cut(s) 199, 353, 361, 571, 821, 872
LweI GCATC 4 cut(s) 29, 266, 287, 920
MaeI CTAG 3 cut(s) 63, 197, 290
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 2 cut(s) 424, 865
MalI GATC 3 cut(s) 74, 472, 851
MbiI CCGCTC 2 cut(s) 508, 840
MboI GATC 3 cut(s) 72, 470, 849
MboII GAAGA 5 cut(s) 20, 263, 744, 859, 906
MflI RGATCY 1 cut(s) 849
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 2 cut(s) 651, 925
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 6 cut(s) 50, 114, 637, 806, 863, 916
Mph1103I ATGCAT 1 cut(s) 241
MseI TTAA 3 cut(s) 116, 336, 824
MspA1I CMGCKG 2 cut(s) 374, 812
MspI CCGG 2 cut(s) 648, 768
MvnI CGCG 1 cut(s) 497
MwoI GCNNNNNNNGC 4 cut(s) 17, 263, 505, 885
NdeI CATATG 1 cut(s) 241
NdeII GATC 3 cut(s) 72, 470, 849
NlaIII CATG 4 cut(s) 84, 229, 548, 571
NlaIV GGNNCC 2 cut(s) 314, 792
NsiI ATGCAT 1 cut(s) 241
PcsI WCGNNNNNNNCGW 1 cut(s) 609
PfeI GAWTC 2 cut(s) 398, 404
PkrI GCNGC 6 cut(s) 214, 343, 376, 561, 811, 887
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
Ppu21I YACGTR 1 cut(s) 132
PpuMI RGGWCCY 1 cut(s) 537
Psp5II RGGWCCY 1 cut(s) 537
PspN4I GGNNCC 2 cut(s) 314, 792
PspPI GGNCC 1 cut(s) 537
PspPPI RGGWCCY 1 cut(s) 537
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 849
PvuII CAGCTG 2 cut(s) 374, 812
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
SaqAI TTAA 3 cut(s) 116, 336, 824
SatI GCNGC 6 cut(s) 213, 342, 375, 560, 810, 886
Sau3AI GATC 3 cut(s) 72, 470, 849
Sau96I GGNCC 1 cut(s) 537
SchI GAGTC 1 cut(s) 34
SduI GDGCHC 1 cut(s) 315
SetI ASST 8 cut(s) 134, 202, 252, 318, 376, 525, 539, 814
SfaNI GCATC 4 cut(s) 29, 266, 287, 920
SfcI CTRYAG 1 cut(s) 213
SgrAI CRCCGGYG 1 cut(s) 767
SinI GGWCC 1 cut(s) 537
SnaBI TACGTA 1 cut(s) 132
Sse9I AATT 2 cut(s) 651, 925
SsiI CCGC 4 cut(s) 264, 508, 663, 840
SspMI CTAG 3 cut(s) 63, 197, 290
TaaI ACNGT 2 cut(s) 181, 760
TaiI ACGT 1 cut(s) 134
TaqI TCGA 1 cut(s) 23
TasI AATT 2 cut(s) 651, 925
TfiI GAWTC 2 cut(s) 398, 404
Tru1I TTAA 3 cut(s) 116, 336, 824
Tru9I TTAA 3 cut(s) 116, 336, 824
TseI GCWGC 6 cut(s) 212, 341, 374, 559, 809, 885
TspDTI ATGAA 2 cut(s) 69, 650
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 2 cut(s) 651, 925
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 202
XspI CTAG 3 cut(s) 63, 197, 290
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.