Rw2G001040

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012527
Physical Location & Seq
Forward (+)
867251 .. 870497
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001040.1

Sequence Viewer

Length: 930 bp
ATGCTTGTAGCGTTTCCTGCATCTAGCCTTCTATCTCGTATCTATTTTGCCAATGGCGGCACTAGCAAGTGGATCATTTCATGGGTGGCTGTTGCAGGGTGGCCTCTGACTGCTTTGATCTTACTGCCTACATACTTCTTCTGCAAAACCTTTCCTACCCCTCTGAATTTAAAACTTACATTATCTTATGCTTTCCTAGGTTTCCTAAGTGCTGCTGACAATCTTATGTATGCATATGCATATGCATACCTCCCAGCATCTACTGCTTCTCTTCTTGCATCATCATCTCTTGTATTTTCTGCATTGTTTGGATATTTACTGGTGAAGAACAAACTCAATGCTTCAATAGTAAATGCTATTGTGATCATTACTGCTGCTATGACCATCATTGCATTGGATTCTGATTCAGACAGATATGACTATGTCACTGAGAAGCAATACATTCTAGGTTTCGTGTGGGATATTTTGGGATCTGCTCTCCATGGGCTCATTTTTGCTCTCTCAGAGCTAGTTTTTGTGAAGCTACTTGGTAGAAGGTCCTTCCATGTTGTGCTGGAGCAACAACTCGGGGTTTCCCTATTTGCGTTTCTGTTTACCACGATCGGGGTCATTTGGAATAAGGATTTTCAGGGAATGGCATCTGAGGCAAGAAGTTTCGAGGGTGGTAAATCTTCTTATTACCTTGTTCTCATTTGGAGTACAATCACTTTCCAGTTGGGGGTGTTAGGAGCTACTGCTGTGCTTTTTCTGTCTTCCACCGTTCTTGCTGGTGTTCTCAATGCAGTAAGGGTACCACTCACAAGCATTGCAGCTGTGATATTGTTAAACGATCCCATGAGCGGCTTAAAGATCCTCTCTTTGATAGTTACATTTTGGGGATTTGGCTGCTACATCTATGGCAGCTCCTCTGGAAGCAGAGATCCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

309

Amino Acids

33.55

Weight (kDa)

8.74

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 2 - 303 8.4e-93 Purine nucleobase transmembrane transport
EamA PF00892 36 - 131 3.4e-08 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 790
AccB1I GGYRCC 1 cut(s) 790
AccBSI CCGCTC 1 cut(s) 840
AciI CCGC 2 cut(s) 57, 840
AclWI GGATC 5 cut(s) 80, 478, 824, 844, 914
AcsI RAATTY 1 cut(s) 166
AfaI GTAC 2 cut(s) 700, 792
AfiI CCNNNNNNNGG 3 cut(s) 603, 718, 839
AgsI TTSAA 1 cut(s) 345
AluBI AGCT 5 cut(s) 508, 523, 731, 812, 903
AluI AGCT 5 cut(s) 508, 523, 731, 812, 903
AlwI GGATC 5 cut(s) 80, 478, 824, 844, 914
Ama87I CYCGRG 1 cut(s) 566
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 5 cut(s) 212, 374, 809, 885, 900
ApoI RAATTY 1 cut(s) 166
Asp718I GGTACC 1 cut(s) 790
AspA2I CCTAGG 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 537
AsuHPI GGTGA 1 cut(s) 334
AvaI CYCGRG 1 cut(s) 566
AvaII GGWCC 1 cut(s) 537
AvrII CCTAGG 1 cut(s) 196
BanI GGYRCC 1 cut(s) 790
BanII GRGCYC 1 cut(s) 489
BbsI GAAGAC 1 cut(s) 744
BbvI GCAGC 5 cut(s) 199, 361, 821, 872, 912
BccI CCATC 1 cut(s) 392
BclI TGATCA 1 cut(s) 363
BfaI CTAG 5 cut(s) 24, 63, 197, 446, 509
BisI GCNGC 7 cut(s) 58, 213, 375, 810, 841, 886, 901
BlnI CCTAGG 1 cut(s) 196
BlsI GCNGC 7 cut(s) 59, 214, 376, 811, 842, 887, 902
Bme18I GGWCC 1 cut(s) 537
BmeT110I CYCGRG 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 537
BmiI GGNNCC 1 cut(s) 792
BmsI GCATC 4 cut(s) 29, 266, 287, 647
BpiI GAAGAC 1 cut(s) 744
BpmI CTGGAG 1 cut(s) 575
BsaJI CCNNGG 2 cut(s) 196, 481
Bsc4I CCNNNNNNNGG 3 cut(s) 603, 718, 839
Bse1I ACTGG 2 cut(s) 324, 712
Bse3DI GCAATG 2 cut(s) 387, 804
BseDI CCNNGG 2 cut(s) 196, 481
BseLI CCNNNNNNNGG 3 cut(s) 603, 718, 839
BseMI GCAATG 2 cut(s) 387, 804
BseMII CTCAG 3 cut(s) 420, 516, 633
BseNI ACTGG 2 cut(s) 324, 712
BseRI GAGGAG 1 cut(s) 895
BseXI GCAGC 5 cut(s) 199, 361, 821, 872, 912
BseYI CCCAGC 1 cut(s) 253
Bsh1285I CGRYCG 1 cut(s) 603
BshFI GGCC 1 cut(s) 103
BshNI GGYRCC 1 cut(s) 790
BsiEI CGRYCG 1 cut(s) 603
BsiHKCI CYCGRG 1 cut(s) 566
BslI CCNNNNNNNGG 3 cut(s) 603, 718, 839
BsnI GGCC 1 cut(s) 103
BsoBI CYCGRG 1 cut(s) 566
Bsp1286I GDGCHC 1 cut(s) 489
Bsp143I GATC 8 cut(s) 72, 117, 363, 470, 600, 829, 849, 919
Bsp19I CCATGG 1 cut(s) 481
BspACI CCGC 2 cut(s) 57, 840
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 3 cut(s) 421, 515, 634
BspLI GGNNCC 1 cut(s) 792
BspPI GGATC 5 cut(s) 80, 478, 824, 844, 914
BspT107I GGYRCC 1 cut(s) 790
BsrBI CCGCTC 1 cut(s) 840
BsrDI GCAATG 2 cut(s) 387, 804
BsrI ACTGG 2 cut(s) 324, 712
BssECI CCNNGG 2 cut(s) 196, 481
BssMI GATC 8 cut(s) 72, 117, 363, 470, 600, 829, 849, 919
BssT1I CCWWGG 2 cut(s) 196, 481
Bst4CI ACNGT 1 cut(s) 760
Bst6I CTCTTC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 263
BstDEI CTNAG 4 cut(s) 206, 429, 502, 642
BstDSI CCRYGG 1 cut(s) 481
BstKTI GATC 8 cut(s) 75, 120, 366, 473, 603, 832, 852, 922
BstMBI GATC 8 cut(s) 72, 117, 363, 470, 600, 829, 849, 919
BstMCI CGRYCG 1 cut(s) 603
BstMWI GCNNNNNNNGC 4 cut(s) 17, 63, 263, 644
BstV1I GCAGC 5 cut(s) 199, 361, 821, 872, 912
BstV2I GAAGAC 1 cut(s) 744
BstX2I RGATCY 3 cut(s) 470, 849, 919
BstYI RGATCY 3 cut(s) 470, 849, 919
BsuRI GGCC 1 cut(s) 103
BtgI CCRYGG 1 cut(s) 481
BtsIMutI CAGTG 1 cut(s) 426
Cfr13I GGNCC 1 cut(s) 537
Csp6I GTAC 2 cut(s) 699, 791
CspCI CAANNNNNGTGG 2 cut(s) 745, 780
CviAII CATG 4 cut(s) 81, 482, 545, 835
CviQI GTAC 2 cut(s) 699, 791
DdeI CTNAG 4 cut(s) 206, 429, 502, 642
DpnI GATC 8 cut(s) 74, 119, 365, 472, 602, 831, 851, 921
DpnII GATC 8 cut(s) 72, 117, 363, 470, 600, 829, 849, 919
DraI TTTAAA 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco130I CCWWGG 2 cut(s) 196, 481
Eco24I GRGCYC 1 cut(s) 489
Eco47I GGWCC 1 cut(s) 537
Eco88I CYCGRG 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 537
EcoT14I CCWWGG 2 cut(s) 196, 481
EcoT22I ATGCAT 3 cut(s) 235, 241, 247
EcoT38I GRGCYC 1 cut(s) 489
ErhI CCWWGG 2 cut(s) 196, 481
FaeI CATG 4 cut(s) 84, 485, 548, 838
FatI CATG 4 cut(s) 80, 481, 544, 834
FauNDI CATATG 2 cut(s) 235, 241
FbaI TGATCA 1 cut(s) 363
Fnu4HI GCNGC 7 cut(s) 58, 213, 375, 810, 841, 886, 901
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 7 cut(s) 58, 213, 375, 810, 841, 886, 901
FspBI CTAG 5 cut(s) 24, 63, 197, 446, 509
GluI GCNGC 7 cut(s) 58, 213, 375, 810, 841, 886, 901
GsaI CCCAGC 1 cut(s) 257
GsuI CTGGAG 1 cut(s) 575
HaeIII GGCC 1 cut(s) 103
Hin1II CATG 4 cut(s) 84, 485, 548, 838
HinfI GANTC 2 cut(s) 398, 404
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 594
Hpy188I TCNGA 6 cut(s) 108, 165, 403, 409, 505, 643
Hpy188III TCNNGA 1 cut(s) 909
Hpy8I GTNNAC 1 cut(s) 594
HpyAV CCTTC 3 cut(s) 38, 528, 550
HpyCH4III ACNGT 1 cut(s) 760
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 63, 263, 644
HpyF3I CTNAG 4 cut(s) 206, 429, 502, 642
Hsp92II CATG 4 cut(s) 84, 485, 548, 838
KpnI GGTACC 1 cut(s) 794
Ksp22I TGATCA 1 cut(s) 363
Kzo9I GATC 8 cut(s) 72, 117, 363, 470, 600, 829, 849, 919
LmnI GCTCC 3 cut(s) 556, 728, 908
LpnPI CCDG 9 cut(s) 30, 81, 267, 305, 539, 614, 725, 753, 894
Lsp1109I GCAGC 5 cut(s) 199, 361, 821, 872, 912
LweI GCATC 4 cut(s) 29, 266, 287, 647
MaeI CTAG 5 cut(s) 24, 63, 197, 446, 509
MaeIII GTNAC 2 cut(s) 424, 865
MalI GATC 8 cut(s) 74, 119, 365, 472, 602, 831, 851, 921
MbiI CCGCTC 1 cut(s) 840
MboI GATC 8 cut(s) 72, 117, 363, 470, 600, 829, 849, 919
MboII GAAGA 5 cut(s) 130, 263, 337, 663, 744
MflI RGATCY 3 cut(s) 470, 849, 919
MhlI GDGCHC 1 cut(s) 489
MluCI AATT 1 cut(s) 166
MnlI CCTC 7 cut(s) 114, 171, 260, 637, 652, 863, 916
Mph1103I ATGCAT 3 cut(s) 235, 241, 247
MseI TTAA 3 cut(s) 170, 824, 845
MspA1I CMGCKG 1 cut(s) 812
MwoI GCNNNNNNNGC 4 cut(s) 17, 63, 263, 644
NcoI CCATGG 1 cut(s) 481
NdeI CATATG 2 cut(s) 235, 241
NdeII GATC 8 cut(s) 72, 117, 363, 470, 600, 829, 849, 919
NlaIII CATG 4 cut(s) 84, 485, 548, 838
NlaIV GGNNCC 1 cut(s) 792
NmuCI GTSAC 1 cut(s) 424
NsiI ATGCAT 3 cut(s) 235, 241, 247
PfeI GAWTC 2 cut(s) 398, 404
PflFI GACNNNGTC 1 cut(s) 422
PkrI GCNGC 7 cut(s) 59, 214, 376, 811, 842, 887, 902
Ple19I CGATCG 1 cut(s) 603
PpuMI RGGWCCY 1 cut(s) 537
Psp5II RGGWCCY 1 cut(s) 537
PspFI CCCAGC 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 792
PspPI GGNCC 1 cut(s) 537
PspPPI RGGWCCY 1 cut(s) 537
PsuI RGATCY 3 cut(s) 470, 849, 919
PsyI GACNNNGTC 1 cut(s) 422
PvuI CGATCG 1 cut(s) 603
PvuII CAGCTG 1 cut(s) 812
RsaI GTAC 2 cut(s) 700, 792
RsaNI GTAC 2 cut(s) 699, 791
SaqAI TTAA 3 cut(s) 170, 824, 845
SatI GCNGC 7 cut(s) 58, 213, 375, 810, 841, 886, 901
Sau3AI GATC 8 cut(s) 72, 117, 363, 470, 600, 829, 849, 919
Sau96I GGNCC 1 cut(s) 537
SduI GDGCHC 1 cut(s) 489
SfaNI GCATC 4 cut(s) 29, 266, 287, 647
SinI GGWCC 1 cut(s) 537
Sse9I AATT 1 cut(s) 166
SsiI CCGC 2 cut(s) 57, 840
SspMI CTAG 5 cut(s) 24, 63, 197, 446, 509
StyI CCWWGG 2 cut(s) 196, 481
TaaI ACNGT 1 cut(s) 760
TaqI TCGA 1 cut(s) 657
TasI AATT 1 cut(s) 166
TatI WGTACW 1 cut(s) 698
TauI GCSGC 2 cut(s) 60, 843
TfiI GAWTC 2 cut(s) 398, 404
Tru1I TTAA 3 cut(s) 170, 824, 845
Tru9I TTAA 3 cut(s) 170, 824, 845
TscAI CASTG 1 cut(s) 433
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 5 cut(s) 212, 374, 809, 885, 900
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 69, 915
TspRI CASTG 1 cut(s) 433
Tth111I GACNNNGTC 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 1 cut(s) 166
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmaJI CCTAGG 1 cut(s) 196
XspI CTAG 5 cut(s) 24, 63, 197, 446, 509
Zsp2I ATGCAT 3 cut(s) 235, 241, 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.