AT2G24320

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009872
Physical Location & Seq
Reverse (-)
10345421 .. 10347053
1633 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G24320.1

Sequence Viewer

Length: 861 bp
ATGGCTGCAAAGTTCGCTTTCTTCCCACCGCCACCAACATACGACGTGGGTAAAGACGAGGAGACCGGAAAGCTTATGTTCACCGGGATAACACCAGAAAAAAGCATGGATGTTCATCAGCTTACCACCAAATCTGGCAACAAGGTCATTGCCACGTTTTGGAAACACCCTTTCTCTAGATTCACTCTTCTTTATTCACATGGCAATGCCGCGGATCTCGGCCAAATGGTTGATCTCTTCATCGAGCTTCGAGCTCATCTCCGCGTCAACATTATGAGCTATGATTATTCAGGCTATGGAGCTTCAACAGGCAAGCCAACTGAGCTCAATACGTACTATGATATCGAGGCTGTGTACAATTGCTTGAGGACCGAGTACGGGATCATGCAAGAGGAAATGATTCTATATGGTCAATCCGTTGGAAGCGGACCAACATTGCACTTAGCATCTCGAGTAAAGAGACTAAGAGGGATTGTTCTTCATAGCGCAATTCTCTCCGGCCTCAGAGTTCTGTATCCTGTCAAAATGACATTCTGGTTCGATATGTACAAAAACATTGACAAGATACGCCATGTAACATGCCCTGTTCTTGTCATACATGGAACAAAAGATGATATTGTGAATATGTCACATGGGAAGCGATTGTGGGAACTAGCAAAGGACAAGTATGATCCATTGTGGGTAAAAGGAGGAGGGCATTGCAACTTGGAAACATACCCCGAGTACATCAAGCATATGCGAAAGTTCATGAACGCCATGGAGAAACTCGCTCTCAACAATCCACCAAACAAACAACAAAACGACGAGCCAAGCATCAAGGAAACTAAACAAAATCGGTGCTTGAGATTCAGCAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4 Peptidase_S9

Protein Analysis

286

Amino Acids

32.85

Weight (kDa)

9.22

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 62 - 166 5.2e-06 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 63 - 180 2.4e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009872)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24320 AT2G24320 AT4G31020 AT4G31020
fragaria_vesca FvH4_1g01310 FvH4_1g01310
malus_domestica MD02G1010900.v1.1 MD15G1155900.v1.1
prunus_persica Prupe.7G259200_v2.0.a1
pyrus_communis pycom02g00980 pycom15g13990
rosa_chinensis RchiOBHm_Chr2g0086141
rosa_laevigata RLG00000015747
rosa_multiflora Rmu_sc0004510.1_g000007
rosa_roxburghii Rroxscaffold_2G00154770
rosa_rugosa Rorug01G0466100
rosa_samantha Rh2AG015900 Rh2BG015900 Rh2CG016700 Rh2DG017900
rosa_wichuraiana Rw2G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 159
AccII CGCG 2 cut(s) 212, 264
AciI CCGC 5 cut(s) 29, 210, 212, 262, 426
AclWI GGATC 3 cut(s) 222, 389, 665
AcoI YGGCCR 1 cut(s) 220
AfaI GTAC 5 cut(s) 335, 356, 377, 548, 725
AfiI CCNNNNNNNGG 2 cut(s) 159, 378
AgsI TTSAA 1 cut(s) 306
AjiI CACGTC 1 cut(s) 46
AluBI AGCT 7 cut(s) 73, 121, 247, 254, 279, 302, 325
AluI AGCT 7 cut(s) 73, 121, 247, 254, 279, 302, 325
Alw21I GWGCWC 2 cut(s) 256, 327
Alw26I GTCTC 2 cut(s) 56, 454
AlwI GGATC 3 cut(s) 222, 389, 665
Ama87I CYCGRG 2 cut(s) 450, 719
AoxI GGCC 2 cut(s) 220, 499
ApeKI GCWGC 1 cut(s) 5
Asp700I GAANNNNTTC 1 cut(s) 399
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 369, 428
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 1 cut(s) 73
AvaI CYCGRG 2 cut(s) 450, 719
AvaII GGWCC 2 cut(s) 369, 428
BanII GRGCYC 2 cut(s) 256, 327
Bbv12I GWGCWC 2 cut(s) 256, 327
BciVI GTATCC 1 cut(s) 525
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 2 cut(s) 56, 454
BfaI CTAG 2 cut(s) 177, 653
BfuI GTATCC 1 cut(s) 525
BisI GCNGC 2 cut(s) 6, 210
BlsI GCNGC 2 cut(s) 7, 211
Bme1390I CCNGG 1 cut(s) 85
Bme18I GGWCC 2 cut(s) 369, 428
BmeT110I CYCGRG 2 cut(s) 450, 719
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 369, 428
BmrFI CCNGG 1 cut(s) 85
BmsI GCATC 2 cut(s) 455, 822
BplI GAGNNNNNCTC 2 cut(s) 243, 275
BpuEI CTTGAG 1 cut(s) 385
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 210, 756
BsaWI WCCGGW 1 cut(s) 65
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 378
Bse3DI GCAATG 4 cut(s) 147, 211, 434, 697
Bse8I GATNNNNATC 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 210, 756
BseGI GGATG 1 cut(s) 115
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 2 cut(s) 159, 378
BseMI GCAATG 4 cut(s) 147, 211, 434, 697
BseMII CTCAG 2 cut(s) 312, 517
BseRI GAGGAG 2 cut(s) 74, 705
Bsh1236I CGCG 2 cut(s) 212, 264
BshFI GGCC 2 cut(s) 222, 501
BsiHKAI GWGCWC 2 cut(s) 256, 327
BsiHKCI CYCGRG 2 cut(s) 450, 719
BsiSI CCGG 3 cut(s) 66, 84, 498
BslI CCNNNNNNNGG 2 cut(s) 159, 378
BsmAI GTCTC 2 cut(s) 56, 454
BsnI GGCC 2 cut(s) 222, 501
Bso31I GGTCTC 1 cut(s) 56
BsoBI CYCGRG 2 cut(s) 450, 719
Bsp1286I GDGCHC 2 cut(s) 256, 327
Bsp1407I TGTACA 2 cut(s) 354, 546
Bsp143I GATC 4 cut(s) 214, 232, 381, 670
Bsp19I CCATGG 1 cut(s) 756
BspACI CCGC 5 cut(s) 29, 210, 212, 262, 426
BspANI GGCC 2 cut(s) 222, 501
BspCNI CTCAG 2 cut(s) 313, 516
BspFNI CGCG 2 cut(s) 212, 264
BspHI TCATGA 1 cut(s) 747
BspPI GGATC 3 cut(s) 222, 389, 665
BspTNI GGTCTC 1 cut(s) 56
BsrDI GCAATG 4 cut(s) 147, 211, 434, 697
BsrGI TGTACA 2 cut(s) 354, 546
BssECI CCNNGG 2 cut(s) 210, 756
BssMI GATC 4 cut(s) 214, 232, 381, 670
BssT1I CCWWGG 1 cut(s) 756
Bst6I CTCTTC 2 cut(s) 192, 242
BstAUI TGTACA 2 cut(s) 354, 546
BstBAI YACGTR 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 314
BstDEI CTNAG 4 cut(s) 321, 442, 464, 503
BstDSI CCRYGG 2 cut(s) 210, 756
BstF5I GGATG 1 cut(s) 115
BstFNI CGCG 2 cut(s) 212, 264
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 4 cut(s) 217, 235, 384, 673
BstMAI GTCTC 2 cut(s) 56, 454
BstMBI GATC 4 cut(s) 214, 232, 381, 670
BstMWI GCNNNNNNNGC 2 cut(s) 14, 322
BstNSI RCATGY 1 cut(s) 582
BstSCI CCNGG 1 cut(s) 83
BstSNI TACGTA 1 cut(s) 333
BstUI CGCG 2 cut(s) 212, 264
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuI GTATCC 1 cut(s) 525
BsuRI GGCC 2 cut(s) 222, 501
BtgI CCRYGG 2 cut(s) 210, 756
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 1 cut(s) 115
Cac8I GCNNGC 1 cut(s) 314
CciI TCATGA 1 cut(s) 747
CfoI GCGC 1 cut(s) 488
Cfr13I GGNCC 2 cut(s) 369, 428
Cfr42I CCGCGG 1 cut(s) 213
CseI GACGC 1 cut(s) 253
Csp6I GTAC 5 cut(s) 334, 355, 376, 547, 724
CviAII CATG 9 cut(s) 106, 200, 385, 572, 579, 599, 632, 748, 757
CviQI GTAC 5 cut(s) 334, 355, 376, 547, 724
DdeI CTNAG 4 cut(s) 321, 442, 464, 503
DpnI GATC 4 cut(s) 216, 234, 383, 672
DpnII GATC 4 cut(s) 214, 232, 381, 670
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 2 cut(s) 192, 242
EarI CTCTTC 2 cut(s) 192, 242
Ecl136II GAGCTC 2 cut(s) 254, 325
Eco105I TACGTA 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 756
Eco24I GRGCYC 2 cut(s) 256, 327
Eco31I GGTCTC 1 cut(s) 56
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 2 cut(s) 369, 428
Eco53kI GAGCTC 2 cut(s) 254, 325
Eco88I CYCGRG 2 cut(s) 450, 719
EcoICRI GAGCTC 2 cut(s) 254, 325
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 1 cut(s) 756
EcoT38I GRGCYC 2 cut(s) 256, 327
ErhI CCWWGG 1 cut(s) 756
FaeI CATG 9 cut(s) 109, 203, 388, 575, 582, 602, 635, 751, 760
FatI CATG 9 cut(s) 105, 199, 384, 571, 578, 598, 631, 747, 756
FauNDI CATATG 1 cut(s) 735
Fnu4HI GCNGC 2 cut(s) 6, 210
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 2 cut(s) 256, 327
Fsp4HI GCNGC 2 cut(s) 6, 210
FspBI CTAG 2 cut(s) 177, 653
GlaI GCGC 1 cut(s) 487
GluI GCNGC 2 cut(s) 6, 210
HaeIII GGCC 2 cut(s) 222, 501
HapII CCGG 3 cut(s) 66, 84, 498
HgaI GACGC 1 cut(s) 253
HhaI GCGC 1 cut(s) 488
Hin1II CATG 9 cut(s) 109, 203, 388, 575, 582, 602, 635, 751, 760
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 3 cut(s) 180, 400, 846
HpaII CCGG 3 cut(s) 66, 84, 498
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 3 cut(s) 81, 268, 355
Hpy188I TCNGA 1 cut(s) 506
Hpy188III TCNNGA 3 cut(s) 177, 450, 748
Hpy8I GTNNAC 3 cut(s) 81, 268, 355
Hpy99I CGWCG 2 cut(s) 47, 806
HpyCH4IV ACGT 3 cut(s) 45, 155, 332
HpyCH4V TGCA 4 cut(s) 8, 388, 439, 702
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 322
HpyF3I CTNAG 4 cut(s) 321, 442, 464, 503
HpySE526I ACGT 3 cut(s) 45, 155, 332
Hsp92II CATG 9 cut(s) 109, 203, 388, 575, 582, 602, 635, 751, 760
HspAI GCGC 1 cut(s) 486
KspI CCGCGG 1 cut(s) 213
Kzo9I GATC 4 cut(s) 214, 232, 381, 670
LmnI GCTCC 1 cut(s) 299
LweI GCATC 2 cut(s) 455, 822
MaeI CTAG 2 cut(s) 177, 653
MaeII ACGT 3 cut(s) 45, 155, 332
MaeIII GTNAC 2 cut(s) 574, 627
MalI GATC 4 cut(s) 216, 234, 383, 672
MboI GATC 4 cut(s) 214, 232, 381, 670
MboII GAAGA 4 cut(s) 13, 179, 229, 470
MfeI CAATTG 1 cut(s) 358
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 2 cut(s) 256, 327
MluCI AATT 2 cut(s) 358, 489
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 8 cut(s) 52, 340, 360, 385, 461, 512, 683, 686
MroXI GAANNNNTTC 1 cut(s) 399
MslI CAYNNNNRTG 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 212
MspI CCGG 3 cut(s) 66, 84, 498
MspR9I CCNGG 1 cut(s) 85
MunI CAATTG 1 cut(s) 358
MvnI CGCG 2 cut(s) 212, 264
MwoI GCNNNNNNNGC 2 cut(s) 14, 322
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 1 cut(s) 756
NdeI CATATG 1 cut(s) 735
NdeII GATC 4 cut(s) 214, 232, 381, 670
NlaIII CATG 9 cut(s) 109, 203, 388, 575, 582, 602, 635, 751, 760
NmeAIII GCCGAG 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 627
NspI RCATGY 1 cut(s) 582
PaeR7I CTCGAG 1 cut(s) 450
PagI TCATGA 1 cut(s) 747
PdmI GAANNNNTTC 1 cut(s) 399
PfeI GAWTC 3 cut(s) 180, 400, 846
PflMI CCANNNNNTGG 1 cut(s) 159
PkrI GCNGC 2 cut(s) 7, 211
Ppu21I YACGTR 1 cut(s) 333
Psp124BI GAGCTC 2 cut(s) 256, 327
PspPI GGNCC 2 cut(s) 369, 428
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 5 cut(s) 335, 356, 377, 548, 725
RsaNI GTAC 5 cut(s) 334, 355, 376, 547, 724
RseI CAYNNNNRTG 1 cut(s) 204
SacI GAGCTC 2 cut(s) 256, 327
SacII CCGCGG 1 cut(s) 213
SatI GCNGC 2 cut(s) 6, 210
Sau3AI GATC 4 cut(s) 214, 232, 381, 670
Sau96I GGNCC 2 cut(s) 369, 428
ScrFI CCNGG 1 cut(s) 85
SduI GDGCHC 2 cut(s) 256, 327
SfaNI GCATC 2 cut(s) 455, 822
Sfr274I CTCGAG 1 cut(s) 450
Sfr303I CCGCGG 1 cut(s) 213
SgrBI CCGCGG 1 cut(s) 213
SinI GGWCC 2 cut(s) 369, 428
SlaI CTCGAG 1 cut(s) 450
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 3 cut(s) 364, 450, 841
SmoI CTYRAG 3 cut(s) 364, 450, 841
SnaBI TACGTA 1 cut(s) 333
Sse9I AATT 2 cut(s) 358, 489
SsiI CCGC 5 cut(s) 29, 210, 212, 262, 426
SspMI CTAG 2 cut(s) 177, 653
SstI GAGCTC 2 cut(s) 256, 327
StyD4I CCNGG 1 cut(s) 83
StyI CCWWGG 1 cut(s) 756
TaiI ACGT 3 cut(s) 48, 158, 335
TaqI TCGA 5 cut(s) 243, 250, 345, 451, 540
TaqII GACCGA 1 cut(s) 386
TasI AATT 2 cut(s) 358, 489
TatI WGTACW 3 cut(s) 354, 546, 723
TauI GCSGC 1 cut(s) 212
TfiI GAWTC 3 cut(s) 180, 400, 846
TseFI GTSAC 1 cut(s) 627
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 627
TspDTI ATGAA 5 cut(s) 104, 229, 470, 736, 764
TspGWI ACGGA 1 cut(s) 406
Van91I CCANNNNNTGG 1 cut(s) 159
VpaK11BI GGWCC 2 cut(s) 369, 428
XbaI TCTAGA 1 cut(s) 176
XceI RCATGY 1 cut(s) 582
XhoI CTCGAG 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 399
XspI CTAG 2 cut(s) 177, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.