AT4G31020

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009872
Physical Location & Seq
Reverse (-)
15108101 .. 15110289
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31020.2

Sequence Viewer

Length: 885 bp
ATGGGTAATGTGACGTCAAATGTGGCGGCCAAGTTCGCATTCTTCCCGCCGGAGCCAGCAACATACGGCGTAACGAAGGATGACGAGACCGGGAAGCTGGTCTTCGCCGGAGTGTCGGCTGACAAGAACGTGGAAGTCCACCAACTCACCACAAAGTCCGGCAACAAAGTTGTTGCCACGTTTTGGAGACACCCTTTTGCGAGATTCACGCTTCTCTATTCCCATGGCAATGCCGCTGATCTTGGCCAAATGGTCGAGCTATTCATCGAACTCCGTGCTCATCTCCGCGTCAACATCATGAGTTATGATTATTCAGGATATGGAGCTTCAACAGGAAAGCCGTCTGAATTCAACACGTATTATGACATAGAGGCAGTGTACAGTTGCTTGCGGAGCGATTATGGGATCAAGCAAGAGGAGATAATTCTGTACGGTCAGTCTGTAGGAAGCGGTCCAACGTTGCACATGGCTTCTCGGTTGAAGAGGCTTAGAGGAGTTGTTCTTCACAGCGCCATTCTCTCTGGAATCAGAGTCTTGTATCCTGTCAAAATGACTCTCTGGTTCGATATATTCAAAAACATAGACAAGATTCGCCATGTCAACTCACAAGTCCTTGTCATACATGGCACAAATGACGAGATTGTTGATTTGTCTCACGGGAAACGACTGTGGGAGCTGGCGAAGGAGAAGTATGATCCGTTATGGGTGAAGGGAGGAGGGCATTGCAACCTCGAGACTTACCCTGAGTACATCAAGCACCTCAAAAAGTTCGTAAATGCAATGGAGAAACTCTCTCTAACCAATCCACCACCCAAACAACTAACTAATGAGCCCAGTATCACCGAGACTAAGCACAATCGGTGCTTGAAATTCGGGAAACGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

294

Amino Acids

33.21

Weight (kDa)

9.2

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 70 - 176 2e-06 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 71 - 187 2.9e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009872)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24320 AT2G24320 AT4G31020 AT4G31020
fragaria_vesca FvH4_1g01310 FvH4_1g01310
malus_domestica MD02G1010900.v1.1 MD15G1155900.v1.1
prunus_persica Prupe.7G259200_v2.0.a1
pyrus_communis pycom02g00980 pycom15g13990
rosa_chinensis RchiOBHm_Chr2g0086141
rosa_laevigata RLG00000015747
rosa_multiflora Rmu_sc0004510.1_g000007
rosa_roxburghii Rroxscaffold_2G00154770
rosa_rugosa Rorug01G0466100
rosa_samantha Rh2AG015900 Rh2BG015900 Rh2CG016700 Rh2DG017900
rosa_wichuraiana Rw2G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 17
AccB7I CCANNNNNTGG 1 cut(s) 183
AccII CGCG 1 cut(s) 288
AciI CCGC 6 cut(s) 26, 47, 234, 286, 391, 450
AclI AACGTT 1 cut(s) 458
AclWI GGATC 2 cut(s) 413, 689
AcoI YGGCCR 2 cut(s) 27, 244
AcsI RAATTY 2 cut(s) 347, 869
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 3 cut(s) 380, 431, 749
AfiI CCNNNNNNNGG 1 cut(s) 183
AflIII ACRYGT 1 cut(s) 354
AgsI TTSAA 5 cut(s) 330, 352, 481, 574, 868
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 4 cut(s) 97, 259, 326, 676
AluI AGCT 4 cut(s) 97, 259, 326, 676
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 5 cut(s) 80, 181, 657, 728, 839
AlwI GGATC 2 cut(s) 413, 689
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 2 cut(s) 27, 244
ApoI RAATTY 2 cut(s) 347, 869
AspLEI GCGC 1 cut(s) 512
AspS9I GGNCC 1 cut(s) 452
AsuC2I CCSGG 1 cut(s) 91
AsuHPI GGTGA 3 cut(s) 139, 718, 832
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 1 cut(s) 452
BalI TGGCCA 1 cut(s) 246
BanII GRGCYC 1 cut(s) 834
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 280
BceAI ACGGC 2 cut(s) 82, 325
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciVI GTATCC 1 cut(s) 549
BcnI CCSGG 1 cut(s) 91
BcoDI GTCTC 5 cut(s) 80, 181, 657, 728, 839
BfmI CTRYAG 1 cut(s) 441
BfoI RGCGCY 1 cut(s) 513
BfuI GTATCC 1 cut(s) 549
BisI GCNGC 2 cut(s) 27, 234
BlsI GCNGC 2 cut(s) 28, 235
Bme1390I CCNGG 1 cut(s) 91
Bme18I GGWCC 1 cut(s) 452
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 91
BmrI ACTGGG 1 cut(s) 828
BmuI ACTGGG 1 cut(s) 828
BpiI GAAGAC 1 cut(s) 94
BplI GAGNNNNNCTC 2 cut(s) 776, 808
BpuMI CCSGG 1 cut(s) 91
BsaAI YACGTR 1 cut(s) 357
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 223
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse1I ACTGG 1 cut(s) 834
Bse3DI GCAATG 3 cut(s) 235, 721, 786
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMI GCAATG 3 cut(s) 235, 721, 786
BseMII CTCAG 1 cut(s) 735
BseNI ACTGG 1 cut(s) 834
BseRI GAGGAG 3 cut(s) 431, 507, 729
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 2 cut(s) 29, 246
BsiHKAI GWGCWC 1 cut(s) 280
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 4 cut(s) 50, 90, 108, 159
BslI CCNNNNNNNGG 1 cut(s) 183
BsmAI GTCTC 5 cut(s) 80, 181, 657, 728, 839
BsmI GAATGC 1 cut(s) 38
BsnI GGCC 2 cut(s) 29, 246
Bso31I GGTCTC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 280, 834
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 3 cut(s) 238, 405, 694
Bsp19I CCATGG 1 cut(s) 223
BspACI CCGC 6 cut(s) 26, 47, 234, 286, 391, 450
BspANI GGCC 2 cut(s) 29, 246
BspCNI CTCAG 1 cut(s) 736
BspFNI CGCG 1 cut(s) 288
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 1 cut(s) 54
BspPI GGATC 2 cut(s) 413, 689
BspTNI GGTCTC 1 cut(s) 80
BsrDI GCAATG 3 cut(s) 235, 721, 786
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 1 cut(s) 834
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 3 cut(s) 238, 405, 694
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 223
Bst4CI ACNGT 4 cut(s) 383, 434, 669, 882
Bst6I CTCTTC 1 cut(s) 476
BstACI GRCGYC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 357
BstC8I GCNNGC 3 cut(s) 57, 389, 678
BstDEI CTNAG 3 cut(s) 488, 744, 849
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 1 cut(s) 85
BstFNI CGCG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 3 cut(s) 241, 408, 697
BstMAI GTCTC 5 cut(s) 80, 181, 657, 728, 839
BstMBI GATC 3 cut(s) 238, 405, 694
BstMWI GCNNNNNNNGC 2 cut(s) 35, 393
BstSCI CCNGG 1 cut(s) 89
BstSFI CTRYAG 1 cut(s) 441
BstUI CGCG 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 94
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 2 cut(s) 29, 246
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 1 cut(s) 85
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 381
Cac8I GCNNGC 3 cut(s) 57, 389, 678
CciI TCATGA 1 cut(s) 297
CfoI GCGC 1 cut(s) 512
Cfr13I GGNCC 1 cut(s) 452
CseI GACGC 1 cut(s) 277
Csp6I GTAC 3 cut(s) 379, 430, 748
CviAII CATG 5 cut(s) 224, 298, 466, 596, 623
CviQI GTAC 3 cut(s) 379, 430, 748
DdeI CTNAG 3 cut(s) 488, 744, 849
DpnI GATC 3 cut(s) 240, 407, 696
DpnII GATC 3 cut(s) 238, 405, 694
EaeI YGGCCR 2 cut(s) 27, 244
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
Eco130I CCWWGG 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 834
Eco31I GGTCTC 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 731
EcoRI GAATTC 1 cut(s) 347
EcoT14I CCWWGG 1 cut(s) 223
EcoT38I GRGCYC 1 cut(s) 834
ErhI CCWWGG 1 cut(s) 223
FaeI CATG 5 cut(s) 227, 301, 469, 599, 626
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FatI CATG 5 cut(s) 223, 297, 465, 595, 622
FauI CCCGC 1 cut(s) 54
Fnu4HI GCNGC 2 cut(s) 27, 234
FokI GGATG 1 cut(s) 92
FriOI GRGCYC 1 cut(s) 834
Fsp4HI GCNGC 2 cut(s) 27, 234
GlaI GCGC 1 cut(s) 511
GluI GCNGC 2 cut(s) 27, 234
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 2 cut(s) 29, 246
HapII CCGG 4 cut(s) 50, 90, 108, 159
HgaI GACGC 1 cut(s) 277
HhaI GCGC 1 cut(s) 512
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 5 cut(s) 227, 301, 469, 599, 626
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HincII GTYRAC 2 cut(s) 292, 601
HindII GTYRAC 2 cut(s) 292, 601
HinfI GANTC 5 cut(s) 204, 525, 531, 553, 589
HpaII CCGG 4 cut(s) 50, 90, 108, 159
HphI GGTGA 3 cut(s) 139, 718, 832
Hpy166II GTNNAC 4 cut(s) 139, 292, 379, 601
Hpy188I TCNGA 2 cut(s) 346, 530
Hpy188III TCNNGA 5 cut(s) 298, 315, 522, 733, 874
Hpy8I GTNNAC 4 cut(s) 139, 292, 379, 601
HpyAV CCTTC 3 cut(s) 70, 676, 703
HpyCH4III ACNGT 4 cut(s) 383, 434, 669, 882
HpyCH4IV ACGT 5 cut(s) 14, 129, 179, 356, 458
HpyCH4V TGCA 3 cut(s) 463, 726, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 393
HpyF3I CTNAG 3 cut(s) 488, 744, 849
HpySE526I ACGT 5 cut(s) 14, 129, 179, 356, 458
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 5 cut(s) 227, 301, 469, 599, 626
HspAI GCGC 1 cut(s) 510
Kzo9I GATC 3 cut(s) 238, 405, 694
LmnI GCTCC 4 cut(s) 52, 323, 393, 673
MaeII ACGT 5 cut(s) 14, 129, 179, 356, 458
MaeIII GTNAC 2 cut(s) 10, 70
MalI GATC 3 cut(s) 240, 407, 696
MboI GATC 3 cut(s) 238, 405, 694
MboII GAAGA 4 cut(s) 34, 94, 493, 494
MhlI GDGCHC 2 cut(s) 280, 834
MlsI TGGCCA 1 cut(s) 246
MluCI AATT 3 cut(s) 347, 423, 869
MluNI TGGCCA 1 cut(s) 246
MlyI GAGTC 2 cut(s) 540, 547
MmeI TCCRAC 1 cut(s) 479
MnlI CCTC 8 cut(s) 364, 409, 477, 485, 707, 710, 740, 770
Mox20I TGGCCA 1 cut(s) 246
MscI TGGCCA 1 cut(s) 246
MslI CAYNNNNRTG 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 246
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 4 cut(s) 50, 90, 108, 159
MspR9I CCNGG 1 cut(s) 91
Mva1269I GAATGC 1 cut(s) 38
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 35, 393
NciI CCSGG 1 cut(s) 91
NcoI CCATGG 1 cut(s) 223
NdeII GATC 3 cut(s) 238, 405, 694
NlaIII CATG 5 cut(s) 227, 301, 469, 599, 626
NlaIV GGNNCC 1 cut(s) 54
NmuCI GTSAC 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 731
PagI TCATGA 1 cut(s) 297
PctI GAATGC 1 cut(s) 38
PfeI GAWTC 3 cut(s) 204, 525, 589
PflMI CCANNNNNTGG 1 cut(s) 183
PkrI GCNGC 2 cut(s) 28, 235
PleI GAGTC 2 cut(s) 539, 547
PpsI GAGTC 2 cut(s) 539, 547
Ppu21I YACGTR 1 cut(s) 357
Psp1406I AACGTT 1 cut(s) 458
PspN4I GGNNCC 1 cut(s) 54
PspPI GGNCC 1 cut(s) 452
RsaI GTAC 3 cut(s) 380, 431, 749
RsaNI GTAC 3 cut(s) 379, 430, 748
RseI CAYNNNNRTG 1 cut(s) 228
SatI GCNGC 2 cut(s) 27, 234
Sau3AI GATC 3 cut(s) 238, 405, 694
Sau96I GGNCC 1 cut(s) 452
SchI GAGTC 2 cut(s) 540, 547
ScrFI CCNGG 1 cut(s) 91
SduI GDGCHC 2 cut(s) 280, 834
SfcI CTRYAG 1 cut(s) 441
Sfr274I CTCGAG 1 cut(s) 731
SinI GGWCC 1 cut(s) 452
SlaI CTCGAG 1 cut(s) 731
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
Sse9I AATT 3 cut(s) 347, 423, 869
SsiI CCGC 6 cut(s) 26, 47, 234, 286, 391, 450
StyD4I CCNGG 1 cut(s) 89
StyI CCWWGG 1 cut(s) 223
TaaI ACNGT 4 cut(s) 383, 434, 669, 882
TaiI ACGT 5 cut(s) 17, 132, 182, 359, 461
TaqI TCGA 4 cut(s) 255, 267, 564, 732
TasI AATT 3 cut(s) 347, 423, 869
TatI WGTACW 2 cut(s) 378, 747
TauI GCSGC 2 cut(s) 29, 236
TfiI GAWTC 3 cut(s) 204, 525, 589
TscAI CASTG 1 cut(s) 381
TseFI GTSAC 1 cut(s) 10
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 1 cut(s) 253
TspGWI ACGGA 2 cut(s) 263, 687
TspRI CASTG 1 cut(s) 381
Van91I CCANNNNNTGG 1 cut(s) 183
VpaK11BI GGWCC 1 cut(s) 452
XapI RAATTY 2 cut(s) 347, 869
XhoI CTCGAG 1 cut(s) 731
ZraI GACGTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.