MD15G1155900.v1.1

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009872
Physical Location & Seq
Reverse (-)
11588152 .. 11591491
3340 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1155900.v1.1.491

Sequence Viewer

Length: 888 bp
ATGGGCAATGTTACGGCGGGAGTGGCGGCCAAGTTTGCGTTTTTCCCACCTGACCCGCCGACGTACGACGTGTACAAGGACGAGGGTGGGAAGCTGGTGTTTTCCAGCGTGACGGCGGACAAGAACATGGAGGTGCATTTGCTGGACACGAAGGGCGGGAACAAGATCGTGGCGACGTTTTGGCGGCACCCTTTAGCGCGGTTCACGCTGCTCTACTCGCATGGGAATGCGGCTGACTTGGGGCAGATGCACGAGCTCTTCATTGAGCTCAGGTCTCACCTGCGTGTCAATATTATGAGCTATGACTATTCGGGCTATGGAGCATCCACTGGTAAGCCATCTGAGTTCAACACATATTATGACATAGAAGCCGTGTACAATTGTTTAAAGAACGAGTATGAAATAAGACAGGAGGATTTGATAGTTTACGGCCAATCTGTTGGAAGTGGACCAACGCTGCACTTGGCTTCTCGCTTAAAAAATCTGAGAGCCGTTGTTCTTCACAGTGCCATCCTTTCAGGCATACGAGTCTTGTATCCTGTCAAGTTGACGTTTTGGTTCGACATTTTCAAAAATATAGACAAAATACGGCATGTCAGCTCTCCGGTTTTAGTTATTCATGGAACAGAGGATGAAATTGTTGACTTGTCCCACGGAAAGCGTTTGTGGGAACTATCCAAAGAAAAGTATGATCCATTATGGGTGAAGGGCGGAGGCCACTGCAACCTTGAAACATATCCCGAGTACATTAAGCACTTACGCAAGTTCATAAACGCAATGGAGAAACTCTCACTCACAAAACCACAACAGGCGAAGAAACAACTCACTTCTGATGGAAGCATCACAGAAGTAAAACACAACAAGTGCTTGAGATTTGGAAAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

296

Amino Acids

33.5

Weight (kDa)

8.99

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 69 - 174 1.3e-06 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 70 - 177 7.2e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009872)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24320 AT2G24320 AT4G31020 AT4G31020
fragaria_vesca FvH4_1g01310 FvH4_1g01310
malus_domestica MD02G1010900.v1.1 MD15G1155900.v1.1
prunus_persica Prupe.7G259200_v2.0.a1
pyrus_communis pycom02g00980 pycom15g13990
rosa_chinensis RchiOBHm_Chr2g0086141
rosa_laevigata RLG00000015747
rosa_multiflora Rmu_sc0004510.1_g000007
rosa_roxburghii Rroxscaffold_2G00154770
rosa_rugosa Rorug01G0466100
rosa_samantha Rh2AG015900 Rh2BG015900 Rh2CG016700 Rh2DG017900
rosa_wichuraiana Rw2G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 288
Acc36I ACCTGC 1 cut(s) 288
AccB1I GGYRCC 1 cut(s) 186
AccII CGCG 1 cut(s) 199
AciI CCGC 9 cut(s) 17, 26, 56, 116, 156, 184, 199, 230, 711
AclWI GGATC 1 cut(s) 686
AcoI YGGCCR 2 cut(s) 27, 430
AfaI GTAC 4 cut(s) 65, 74, 377, 746
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 3 cut(s) 349, 571, 731
AjiI CACGTC 1 cut(s) 70
AleI CACNNNNGTG 1 cut(s) 282
AluBI AGCT 5 cut(s) 94, 256, 268, 300, 600
AluI AGCT 5 cut(s) 94, 256, 268, 300, 600
Alw21I GWGCWC 2 cut(s) 258, 270
Alw26I GTCTC 1 cut(s) 279
AlwI GGATC 1 cut(s) 686
Ama87I CYCGRG 1 cut(s) 740
AoxI GGCC 3 cut(s) 27, 430, 715
ApeKI GCWGC 2 cut(s) 208, 457
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 1 cut(s) 449
AsuHPI GGTGA 2 cut(s) 269, 715
AvaI CYCGRG 1 cut(s) 740
AvaII GGWCC 1 cut(s) 449
BanI GGYRCC 1 cut(s) 186
BanII GRGCYC 2 cut(s) 258, 270
BauI CACGAG 1 cut(s) 251
Bbv12I GWGCWC 2 cut(s) 258, 270
BbvI GCAGC 2 cut(s) 195, 444
BccI CCATC 3 cut(s) 346, 518, 827
BceAI ACGGC 6 cut(s) 30, 129, 356, 445, 476, 605
BciVI GTATCC 1 cut(s) 546
BcoDI GTCTC 1 cut(s) 279
BfuAI ACCTGC 1 cut(s) 288
BfuI GTATCC 1 cut(s) 546
BisI GCNGC 5 cut(s) 27, 185, 209, 231, 458
BlsI GCNGC 5 cut(s) 28, 186, 210, 232, 459
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 740
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 188
BmsI GCATC 3 cut(s) 237, 332, 849
BplI GAGNNNNNCTC 2 cut(s) 773, 805
Bpu10I CCTNAGC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 279
BsaJI CCNNGG 1 cut(s) 652
BsaWI WCCGGW 1 cut(s) 604
Bse1I ACTGG 1 cut(s) 334
Bse3DI GCAATG 2 cut(s) 13, 783
BseDI CCNNGG 1 cut(s) 652
BseGI GGATG 3 cut(s) 323, 510, 637
BseMI GCAATG 2 cut(s) 13, 783
BseMII CTCAG 3 cut(s) 283, 333, 476
BseNI ACTGG 1 cut(s) 334
BseXI GCAGC 2 cut(s) 195, 444
BsgI GTGCAG 1 cut(s) 443
Bsh1236I CGCG 1 cut(s) 199
BshFI GGCC 3 cut(s) 29, 432, 717
BshNI GGYRCC 1 cut(s) 186
BsiHKAI GWGCWC 2 cut(s) 258, 270
BsiHKCI CYCGRG 1 cut(s) 740
BsiSI CCGG 1 cut(s) 605
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 634
BsmAI GTCTC 1 cut(s) 279
BsmFI GGGAC 1 cut(s) 634
BsmI GAATGC 1 cut(s) 232
BsnI GGCC 3 cut(s) 29, 432, 717
Bso31I GGTCTC 1 cut(s) 279
BsoBI CYCGRG 1 cut(s) 740
Bsp1286I GDGCHC 2 cut(s) 258, 270
Bsp1407I TGTACA 2 cut(s) 72, 375
Bsp143I GATC 2 cut(s) 165, 691
BspACI CCGC 9 cut(s) 17, 26, 56, 116, 156, 184, 199, 230, 711
BspANI GGCC 3 cut(s) 29, 432, 717
BspCNI CTCAG 3 cut(s) 282, 334, 477
BspFNI CGCG 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 188
BspMI ACCTGC 1 cut(s) 288
BspPI GGATC 1 cut(s) 686
BspQI GCTCTTC 1 cut(s) 263
BspT107I GGYRCC 1 cut(s) 186
BspTNI GGTCTC 1 cut(s) 279
BsrDI GCAATG 2 cut(s) 13, 783
BsrGI TGTACA 2 cut(s) 72, 375
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 652
BssMI GATC 2 cut(s) 165, 691
BssSI CACGAG 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 251
Bst4CI ACNGT 1 cut(s) 506
Bst6I CTCTTC 1 cut(s) 263
BstAUI TGTACA 2 cut(s) 72, 375
BstDEI CTNAG 3 cut(s) 269, 342, 485
BstDSI CCRYGG 1 cut(s) 652
BstF5I GGATG 3 cut(s) 323, 510, 637
BstFNI CGCG 1 cut(s) 199
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 2 cut(s) 168, 694
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 2 cut(s) 165, 691
BstMWI GCNNNNNNNGC 4 cut(s) 23, 35, 205, 217
BstNSI RCATGY 1 cut(s) 596
BstUI CGCG 1 cut(s) 199
BstV1I GCAGC 2 cut(s) 195, 444
BstXI CCANNNNNNTGG 1 cut(s) 440
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 3 cut(s) 29, 432, 717
BtgI CCRYGG 1 cut(s) 652
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 3 cut(s) 323, 510, 637
BtsI GCAGTG 1 cut(s) 718
BtsIMutI CAGTG 3 cut(s) 327, 511, 718
BveI ACCTGC 1 cut(s) 288
CfoI GCGC 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 449
Csp6I GTAC 4 cut(s) 64, 73, 376, 745
CviAII CATG 4 cut(s) 127, 221, 593, 620
CviQI GTAC 4 cut(s) 64, 73, 376, 745
DdeI CTNAG 3 cut(s) 269, 342, 485
DpnI GATC 2 cut(s) 167, 693
DpnII GATC 2 cut(s) 165, 691
DraI TTTAAA 1 cut(s) 387
EaeI YGGCCR 2 cut(s) 27, 430
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EciI GGCGGA 2 cut(s) 131, 726
Ecl136II GAGCTC 2 cut(s) 256, 268
Eco24I GRGCYC 2 cut(s) 258, 270
Eco31I GGTCTC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 449
Eco53kI GAGCTC 2 cut(s) 256, 268
Eco88I CYCGRG 1 cut(s) 740
EcoICRI GAGCTC 2 cut(s) 256, 268
EcoT38I GRGCYC 2 cut(s) 258, 270
FaeI CATG 4 cut(s) 130, 224, 596, 623
FaqI GGGAC 1 cut(s) 634
FatI CATG 4 cut(s) 126, 220, 592, 619
FauI CCCGC 3 cut(s) 10, 63, 149
Fnu4HI GCNGC 5 cut(s) 27, 185, 209, 231, 458
FokI GGATG 3 cut(s) 310, 497, 644
FriOI GRGCYC 2 cut(s) 258, 270
Fsp4HI GCNGC 5 cut(s) 27, 185, 209, 231, 458
GlaI GCGC 1 cut(s) 198
GluI GCNGC 5 cut(s) 27, 185, 209, 231, 458
HaeIII GGCC 3 cut(s) 29, 432, 717
HapII CCGG 1 cut(s) 605
HhaI GCGC 1 cut(s) 199
Hin1II CATG 4 cut(s) 130, 224, 596, 623
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HincII GTYRAC 2 cut(s) 549, 643
HindII GTYRAC 2 cut(s) 549, 643
HinfI GANTC 1 cut(s) 528
HpaII CCGG 1 cut(s) 605
HphI GGTGA 2 cut(s) 269, 715
Hpy166II GTNNAC 7 cut(s) 73, 204, 376, 427, 449, 549, 643
Hpy188I TCNGA 3 cut(s) 343, 486, 832
Hpy188III TCNNGA 1 cut(s) 740
Hpy8I GTNNAC 7 cut(s) 73, 204, 376, 427, 449, 549, 643
Hpy99I CGWCG 3 cut(s) 64, 71, 178
HpyAV CCTTC 2 cut(s) 145, 700
HpyCH4III ACNGT 1 cut(s) 506
HpyCH4IV ACGT 4 cut(s) 62, 69, 176, 551
HpyCH4V TGCA 4 cut(s) 136, 250, 460, 723
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 35, 205, 217
HpyF3I CTNAG 3 cut(s) 269, 342, 485
HpySE526I ACGT 4 cut(s) 62, 69, 176, 551
Hsp92II CATG 4 cut(s) 130, 224, 596, 623
HspAI GCGC 1 cut(s) 197
Kzo9I GATC 2 cut(s) 165, 691
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 2 cut(s) 195, 444
LweI GCATC 3 cut(s) 237, 332, 849
MaeII ACGT 4 cut(s) 62, 69, 176, 551
MaeIII GTNAC 2 cut(s) 10, 109
MalI GATC 2 cut(s) 167, 693
MboI GATC 2 cut(s) 165, 691
MboII GAAGA 3 cut(s) 250, 491, 826
MfeI CAATTG 1 cut(s) 379
MhlI GDGCHC 2 cut(s) 258, 270
MluCI AATT 2 cut(s) 379, 636
MlyI GAGTC 1 cut(s) 537
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 6 cut(s) 76, 124, 406, 622, 707, 876
MseI TTAA 3 cut(s) 386, 476, 750
MslI CAYNNNNRTG 3 cut(s) 131, 225, 282
MspI CCGG 1 cut(s) 605
MunI CAATTG 1 cut(s) 379
Mva1269I GAATGC 1 cut(s) 232
MvnI CGCG 1 cut(s) 199
MwoI GCNNNNNNNGC 4 cut(s) 23, 35, 205, 217
NdeII GATC 2 cut(s) 165, 691
NlaIII CATG 4 cut(s) 130, 224, 596, 623
NlaIV GGNNCC 1 cut(s) 188
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 1 cut(s) 596
OliI CACNNNNGTG 1 cut(s) 282
PaqCI CACCTGC 1 cut(s) 288
PciSI GCTCTTC 1 cut(s) 263
PctI GAATGC 1 cut(s) 232
Pfl23II CGTACG 1 cut(s) 63
PkrI GCNGC 5 cut(s) 28, 186, 210, 232, 459
PleI GAGTC 1 cut(s) 536
PpsI GAGTC 1 cut(s) 536
Psp124BI GAGCTC 2 cut(s) 258, 270
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 188
PspPI GGNCC 1 cut(s) 449
RsaI GTAC 4 cut(s) 65, 74, 377, 746
RsaNI GTAC 4 cut(s) 64, 73, 376, 745
RseI CAYNNNNRTG 3 cut(s) 131, 225, 282
SacI GAGCTC 2 cut(s) 258, 270
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 3 cut(s) 386, 476, 750
SatI GCNGC 5 cut(s) 27, 185, 209, 231, 458
Sau3AI GATC 2 cut(s) 165, 691
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 1 cut(s) 537
SduI GDGCHC 2 cut(s) 258, 270
SfaNI GCATC 3 cut(s) 237, 332, 849
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 3 cut(s) 131, 225, 282
SmlI CTYRAG 1 cut(s) 868
SmoI CTYRAG 1 cut(s) 868
Sse9I AATT 2 cut(s) 379, 636
SsiI CCGC 9 cut(s) 17, 26, 56, 116, 156, 184, 199, 230, 711
SspI AATATT 1 cut(s) 292
SstI GAGCTC 2 cut(s) 258, 270
TaaI ACNGT 1 cut(s) 506
TaiI ACGT 4 cut(s) 65, 72, 179, 554
TaqI TCGA 1 cut(s) 561
TasI AATT 2 cut(s) 379, 636
TatI WGTACW 3 cut(s) 72, 375, 744
TauI GCSGC 3 cut(s) 29, 187, 233
Tru1I TTAA 3 cut(s) 386, 476, 750
Tru9I TTAA 3 cut(s) 386, 476, 750
TscAI CASTG 3 cut(s) 334, 511, 725
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 2 cut(s) 208, 457
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 5 cut(s) 250, 414, 608, 648, 757
TspGWI ACGGA 1 cut(s) 669
TspRI CASTG 3 cut(s) 334, 511, 725
VpaK11BI GGWCC 1 cut(s) 449
XceI RCATGY 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.