Rh2DG017900

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009872
Physical Location & Seq
Reverse (-)
1030813 .. 1034289
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG017900.1

Sequence Viewer

Length: 672 bp
ATGGGCAATGTAACGTCTGGTGTGGCTGCGAAGTTTGCGTTCTTTCCACCAGACCCGCCGACTTATGATGTGTCGAAAGACGAAGGAGGGAAGCTTGTGTTCTCTGGAGTCTCCGGTGATAAGAACATGGATGTGCATTTGGTTGAGACCAAAGGAGGAAACAAAGTCGTTGCCACGTTTTGGAAGCACCCTTTTGCCAGGTTCACCATTCTTTACTCGCATGGCAATGCTGCTGATTTGGGTCAGATGCATGAGCTCTTCATTGAGCTCAGGGCTCACCTCAGGGTTAATATCATGAGCTATGATTATTCAGGATATGGAGCATCCAGCGGTAAGCCATCTGAGTTCAACACCTATCATGACATAGAAGCTGTGTACAATTGCTTAAAGAGCGAATATGGAGTAAAGCAAGAAGATTTGATACTTTACGGCCAATCTGTTGGAAGTGGACCAACACTGCACATGGCTTCTCGGTTAAAGAGGTTGAGAGCTGTTGTTCTCCATAGTGCAATCCTTTCAGGCATACGAGTCTTGTATAATATCAAAGTGACATTTTGGTTCGACATTTTTAAAAATATAGACAAAATACGGCATGTCACCTGTCCGGTTTTAGTTATACATGTAAGTACCTCTTCCCTTCCTCCTGTAATTTTCTTTAGTTCTGTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

223

Amino Acids

24.73

Weight (kDa)

8.76

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 68 - 173 1.6e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 70 - 170 1e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009872)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24320 AT2G24320 AT4G31020 AT4G31020
fragaria_vesca FvH4_1g01310 FvH4_1g01310
malus_domestica MD02G1010900.v1.1 MD15G1155900.v1.1
prunus_persica Prupe.7G259200_v2.0.a1
pyrus_communis pycom02g00980 pycom15g13990
rosa_chinensis RchiOBHm_Chr2g0086141
rosa_laevigata RLG00000015747
rosa_multiflora Rmu_sc0004510.1_g000007
rosa_roxburghii Rroxscaffold_2G00154770
rosa_rugosa Rorug01G0466100
rosa_samantha Rh2AG015900 Rh2BG015900 Rh2CG016700 Rh2DG017900
rosa_wichuraiana Rw2G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 180
AciI CCGC 2 cut(s) 56, 330
AcoI YGGCCR 1 cut(s) 430
AfaI GTAC 2 cut(s) 377, 628
AfiI CCNNNNNNNGG 1 cut(s) 180
AflIII ACRYGT 1 cut(s) 619
AgsI TTSAA 1 cut(s) 349
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 6 cut(s) 94, 256, 268, 300, 371, 491
AluI AGCT 6 cut(s) 94, 256, 268, 300, 371, 491
Alw21I GWGCWC 2 cut(s) 258, 270
Alw26I GTCTC 2 cut(s) 115, 140
AoxI GGCC 1 cut(s) 430
ApeKI GCWGC 2 cut(s) 26, 230
AspS9I GGNCC 1 cut(s) 449
AsuHPI GGTGA 4 cut(s) 128, 196, 269, 589
AvaII GGWCC 1 cut(s) 449
AxyI CCTNAGG 1 cut(s) 281
BanII GRGCYC 3 cut(s) 258, 270, 277
BarI GAAGNNNNNNTAC 2 cut(s) 405, 437
Bbv12I GWGCWC 2 cut(s) 258, 270
BbvI GCAGC 2 cut(s) 13, 217
BccI CCATC 1 cut(s) 346
BceAI ACGGC 2 cut(s) 445, 605
BciT130I CCWGG 1 cut(s) 199
BcoDI GTCTC 2 cut(s) 115, 140
BisI GCNGC 2 cut(s) 27, 231
BlsI GCNGC 2 cut(s) 28, 232
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 1 cut(s) 449
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 2 cut(s) 237, 332
BpmI CTGGAG 1 cut(s) 126
Bpu10I CCTNAGC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 140
BsaWI WCCGGW 2 cut(s) 113, 604
Bsc4I CCNNNNNNNGG 1 cut(s) 180
Bse21I CCTNAGG 1 cut(s) 281
Bse3DI GCAATG 2 cut(s) 13, 232
BseBI CCWGG 1 cut(s) 199
BseGI GGATG 2 cut(s) 136, 323
BseLI CCNNNNNNNGG 1 cut(s) 180
BseMI GCAATG 2 cut(s) 13, 232
BseMII CTCAG 3 cut(s) 283, 295, 333
BseXI GCAGC 2 cut(s) 13, 217
BsgI GTGCAG 1 cut(s) 443
BshFI GGCC 1 cut(s) 432
BsiHKAI GWGCWC 2 cut(s) 258, 270
BsiSI CCGG 2 cut(s) 114, 605
BslI CCNNNNNNNGG 1 cut(s) 180
BsmAI GTCTC 2 cut(s) 115, 140
BsnI GGCC 1 cut(s) 432
Bso31I GGTCTC 1 cut(s) 140
Bsp1286I GDGCHC 3 cut(s) 258, 270, 277
Bsp1407I TGTACA 1 cut(s) 375
BspACI CCGC 2 cut(s) 56, 330
BspANI GGCC 1 cut(s) 432
BspCNI CTCAG 3 cut(s) 282, 294, 334
BspHI TCATGA 2 cut(s) 294, 358
BspQI GCTCTTC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 140
BsrDI GCAATG 2 cut(s) 13, 232
BsrGI TGTACA 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 199
Bst6I CTCTTC 2 cut(s) 263, 637
BstAUI TGTACA 1 cut(s) 375
BstDEI CTNAG 3 cut(s) 269, 281, 342
BstF5I GGATG 2 cut(s) 136, 323
BstMAI GTCTC 2 cut(s) 115, 140
BstMWI GCNNNNNNNGC 2 cut(s) 35, 390
BstNI CCWGG 1 cut(s) 199
BstNSI RCATGY 2 cut(s) 596, 623
BstSCI CCNGG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 13, 217
BstXI CCANNNNNNTGG 1 cut(s) 440
Bsu36I CCTNAGG 1 cut(s) 281
BsuRI GGCC 1 cut(s) 432
BtsCI GGATG 2 cut(s) 136, 323
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 1 cut(s) 455
CciI TCATGA 2 cut(s) 294, 358
Cfr13I GGNCC 1 cut(s) 449
Csp6I GTAC 2 cut(s) 376, 627
CviAII CATG 8 cut(s) 127, 221, 251, 295, 359, 463, 593, 620
CviQI GTAC 2 cut(s) 376, 627
DdeI CTNAG 3 cut(s) 269, 281, 342
DraI TTTAAA 1 cut(s) 571
EaeI YGGCCR 1 cut(s) 430
Eam1104I CTCTTC 2 cut(s) 263, 637
EarI CTCTTC 2 cut(s) 263, 637
Ecl136II GAGCTC 2 cut(s) 256, 268
Eco24I GRGCYC 3 cut(s) 258, 270, 277
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 1 cut(s) 449
Eco53kI GAGCTC 2 cut(s) 256, 268
Eco81I CCTNAGG 1 cut(s) 281
EcoICRI GAGCTC 2 cut(s) 256, 268
EcoRII CCWGG 1 cut(s) 197
EcoT22I ATGCAT 1 cut(s) 252
EcoT38I GRGCYC 3 cut(s) 258, 270, 277
FaeI CATG 8 cut(s) 130, 224, 254, 298, 362, 466, 596, 623
FalI AAGNNNNNCTT 2 cut(s) 616, 648
FatI CATG 8 cut(s) 126, 220, 250, 294, 358, 462, 592, 619
FauI CCCGC 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 27, 231
FokI GGATG 2 cut(s) 143, 310
FriOI GRGCYC 3 cut(s) 258, 270, 277
Fsp4HI GCNGC 2 cut(s) 27, 231
GluI GCNGC 2 cut(s) 27, 231
GsuI CTGGAG 1 cut(s) 126
HaeIII GGCC 1 cut(s) 432
HapII CCGG 2 cut(s) 114, 605
Hin1II CATG 8 cut(s) 130, 224, 254, 298, 362, 466, 596, 623
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 2 cut(s) 108, 528
HpaII CCGG 2 cut(s) 114, 605
HphI GGTGA 4 cut(s) 128, 196, 269, 589
Hpy166II GTNNAC 4 cut(s) 204, 376, 449, 667
Hpy188I TCNGA 2 cut(s) 246, 343
Hpy188III TCNNGA 4 cut(s) 105, 295, 312, 359
Hpy8I GTNNAC 4 cut(s) 204, 376, 449, 667
HpyAV CCTTC 2 cut(s) 77, 647
HpyCH4IV ACGT 2 cut(s) 14, 176
HpyCH4V TGCA 4 cut(s) 136, 250, 460, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 390
HpyF3I CTNAG 3 cut(s) 269, 281, 342
HpySE526I ACGT 2 cut(s) 14, 176
Hsp92II CATG 8 cut(s) 130, 224, 254, 298, 362, 466, 596, 623
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 2 cut(s) 13, 217
LweI GCATC 2 cut(s) 237, 332
MaeII ACGT 2 cut(s) 14, 176
MaeIII GTNAC 3 cut(s) 10, 547, 595
MboII GAAGA 3 cut(s) 250, 425, 624
MfeI CAATTG 1 cut(s) 379
MhlI GDGCHC 3 cut(s) 258, 270, 277
MluCI AATT 2 cut(s) 379, 648
MlyI GAGTC 2 cut(s) 117, 537
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 6 cut(s) 80, 149, 290, 474, 640, 651
Mph1103I ATGCAT 1 cut(s) 252
MseI TTAA 4 cut(s) 288, 386, 476, 570
MslI CAYNNNNRTG 2 cut(s) 131, 225
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 2 cut(s) 114, 605
MspR9I CCNGG 1 cut(s) 199
MunI CAATTG 1 cut(s) 379
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 2 cut(s) 35, 390
NlaIII CATG 8 cut(s) 130, 224, 254, 298, 362, 466, 596, 623
NmuCI GTSAC 2 cut(s) 547, 595
NsiI ATGCAT 1 cut(s) 252
NspI RCATGY 2 cut(s) 596, 623
PagI TCATGA 2 cut(s) 294, 358
PciI ACATGT 1 cut(s) 619
PciSI GCTCTTC 1 cut(s) 263
PflMI CCANNNNNTGG 1 cut(s) 180
PkrI GCNGC 2 cut(s) 28, 232
PleI GAGTC 2 cut(s) 116, 536
PpsI GAGTC 2 cut(s) 116, 536
PscI ACATGT 1 cut(s) 619
Psp124BI GAGCTC 2 cut(s) 258, 270
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspPI GGNCC 1 cut(s) 449
RsaI GTAC 2 cut(s) 377, 628
RsaNI GTAC 2 cut(s) 376, 627
RseI CAYNNNNRTG 2 cut(s) 131, 225
SacI GAGCTC 2 cut(s) 258, 270
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 4 cut(s) 288, 386, 476, 570
SatI GCNGC 2 cut(s) 27, 231
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 2 cut(s) 117, 537
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 3 cut(s) 258, 270, 277
SfaNI GCATC 2 cut(s) 237, 332
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 2 cut(s) 131, 225
Sse9I AATT 2 cut(s) 379, 648
SsiI CCGC 2 cut(s) 56, 330
SstI GAGCTC 2 cut(s) 258, 270
StyD4I CCNGG 1 cut(s) 197
TaiI ACGT 2 cut(s) 17, 179
TaqI TCGA 2 cut(s) 74, 561
TasI AATT 2 cut(s) 379, 648
TatI WGTACW 1 cut(s) 375
Tru1I TTAA 4 cut(s) 288, 386, 476, 570
Tru9I TTAA 4 cut(s) 288, 386, 476, 570
TscAI CASTG 1 cut(s) 462
TseFI GTSAC 2 cut(s) 547, 595
TseI GCWGC 2 cut(s) 26, 230
Tsp45I GTSAC 2 cut(s) 547, 595
TspDTI ATGAA 1 cut(s) 250
TspRI CASTG 1 cut(s) 462
Van91I CCANNNNNTGG 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 449
XceI RCATGY 2 cut(s) 596, 623
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.