FvH4_1g01310

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009872
Physical Location & Seq
Reverse (-)
654666 .. 658135
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01310.t1

Sequence Viewer

Length: 879 bp
ATGGGCAATGTAACGTCTGGTGTGGCTGCAAAGTTTGCGTTTTTTCCACCAGACCCGCCGACGTATCATGTGTCGAAAGACGAGGGAGGGAAGCTTGTGTTCTCTGGAGTCTCGGCTGATAAGAACATGGAGCCTCATTTGCTTGAGACCAAAGCCGGAAACAGAGTCGTGGCCACGTTTTGGAAGCACCCTTTTGCAAGGTTCACCATTCTTTACTCGCATGGCAATGCTGCTGATTTGGGTCAGATGCATGAGCTCTTCATTGAGCTCAGGGCTCACCTCAGGGTTAATATTATGAGCTACGATTATTCAGGATATGGAGCATCCAGCGGTAAGCCATCCGAGTTCAACACCTATCACGACATAGAAGCTGTGTACAATTGCTTGAAGACCGAATATGGAGTAAAGCAAGAAGATTTGATACTTTACGGCCAATCTGTTGGAAGCGGACCAACACTGCACATGGGTTCTCGGTTAAAGAGACTGAGAGCTGTTGTTCTTCATAGTGCAATCCTCTCAGGCATACGAGTTTTATATAATATCAAAGTGACATTTTGGTTCGACATTTTCAAAAATATAGACAAAATACGGCATGTCAGCTGCCCAGTTTTAGTTATACATGGAACAGAAGATGACATTGTCGATTTGTCCCATGGGAAGCGTCTATGGGAACTTTCCAAAGAAAAATACGACCCCTTATGGGTCAAGGGCGGAGGCCATTGCAACCTAGAAACGTATCCAGAGTACATTAAGCACTTACGCAAGTTCATCAATGCGATGGAGAAACTCTCGCTCTCGAATCCATCGAAGAAAGAACAGACTCCTACCCCGAGTATGGAATTAAAACACAACAAGTGCTTGAGATTTTCCAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

293

Amino Acids

33.07

Weight (kDa)

9.14

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 68 - 173 5.6e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 70 - 170 5e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009872)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24320 AT2G24320 AT4G31020 AT4G31020
fragaria_vesca FvH4_1g01310 FvH4_1g01310
malus_domestica MD02G1010900.v1.1 MD15G1155900.v1.1
prunus_persica Prupe.7G259200_v2.0.a1
pyrus_communis pycom02g00980 pycom15g13990
rosa_chinensis RchiOBHm_Chr2g0086141
rosa_laevigata RLG00000015747
rosa_multiflora Rmu_sc0004510.1_g000007
rosa_roxburghii Rroxscaffold_2G00154770
rosa_rugosa Rorug01G0466100
rosa_samantha Rh2AG015900 Rh2BG015900 Rh2CG016700 Rh2DG017900
rosa_wichuraiana Rw2G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 180
AciI CCGC 4 cut(s) 56, 330, 447, 711
AcoI YGGCCR 2 cut(s) 171, 430
AfaI GTAC 2 cut(s) 377, 746
AfiI CCNNNNNNNGG 3 cut(s) 180, 700, 835
AgsI TTSAA 3 cut(s) 349, 388, 571
AluBI AGCT 7 cut(s) 94, 256, 268, 300, 371, 491, 600
AluI AGCT 7 cut(s) 94, 256, 268, 300, 371, 491, 600
Alw21I GWGCWC 2 cut(s) 258, 270
Alw26I GTCTC 3 cut(s) 115, 140, 475
Ama87I CYCGRG 1 cut(s) 829
AoxI GGCC 3 cut(s) 171, 430, 715
ApeKI GCWGC 3 cut(s) 26, 230, 600
AspS9I GGNCC 1 cut(s) 449
AsuHPI GGTGA 2 cut(s) 196, 269
AvaI CYCGRG 1 cut(s) 829
AvaII GGWCC 1 cut(s) 449
AxyI CCTNAGG 1 cut(s) 281
BalI TGGCCA 1 cut(s) 173
BanII GRGCYC 3 cut(s) 258, 270, 277
BarI GAAGNNNNNNTAC 2 cut(s) 405, 437
BbsI GAAGAC 1 cut(s) 395
Bbv12I GWGCWC 2 cut(s) 258, 270
BbvI GCAGC 3 cut(s) 13, 217, 587
BccI CCATC 3 cut(s) 346, 772, 811
BceAI ACGGC 2 cut(s) 445, 605
BciVI GTATCC 1 cut(s) 747
BcoDI GTCTC 3 cut(s) 115, 140, 475
BfaI CTAG 1 cut(s) 728
BfuI GTATCC 1 cut(s) 747
BisI GCNGC 3 cut(s) 27, 231, 601
BlsI GCNGC 3 cut(s) 28, 232, 602
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 829
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 132
BmrI ACTGGG 1 cut(s) 599
BmsI GCATC 2 cut(s) 237, 332
BmuI ACTGGG 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 395
BplI GAGNNNNNCTC 2 cut(s) 773, 805
BpmI CTGGAG 1 cut(s) 126
Bpu10I CCTNAGC 1 cut(s) 269
BpuEI CTTGAG 1 cut(s) 164
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 652
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 700, 835
Bse1I ACTGG 1 cut(s) 605
Bse21I CCTNAGG 1 cut(s) 281
Bse3DI GCAATG 3 cut(s) 13, 232, 718
BseDI CCNNGG 1 cut(s) 652
BseGI GGATG 2 cut(s) 323, 338
BseLI CCNNNNNNNGG 3 cut(s) 180, 700, 835
BseMI GCAATG 3 cut(s) 13, 232, 718
BseMII CTCAG 4 cut(s) 283, 295, 476, 531
BseNI ACTGG 1 cut(s) 605
BseXI GCAGC 3 cut(s) 13, 217, 587
BsgI GTGCAG 1 cut(s) 443
BshFI GGCC 3 cut(s) 173, 432, 717
BsiHKAI GWGCWC 2 cut(s) 258, 270
BsiHKCI CYCGRG 1 cut(s) 829
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 634
BslI CCNNNNNNNGG 3 cut(s) 180, 700, 835
BsmAI GTCTC 3 cut(s) 115, 140, 475
BsmFI GGGAC 1 cut(s) 634
BsnI GGCC 3 cut(s) 173, 432, 717
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 829
Bsp1286I GDGCHC 3 cut(s) 258, 270, 277
Bsp1407I TGTACA 1 cut(s) 375
Bsp19I CCATGG 1 cut(s) 652
BspACI CCGC 4 cut(s) 56, 330, 447, 711
BspANI GGCC 3 cut(s) 173, 432, 717
BspCNI CTCAG 4 cut(s) 282, 294, 477, 530
BspLI GGNNCC 1 cut(s) 132
BspQI GCTCTTC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 140
BsrDI GCAATG 3 cut(s) 13, 232, 718
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 1 cut(s) 605
BssECI CCNNGG 1 cut(s) 652
BssT1I CCWWGG 1 cut(s) 652
Bst6I CTCTTC 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 35
BstAUI TGTACA 1 cut(s) 375
BstDEI CTNAG 4 cut(s) 269, 281, 485, 517
BstDSI CCRYGG 1 cut(s) 652
BstF5I GGATG 2 cut(s) 323, 338
BstMAI GTCTC 3 cut(s) 115, 140, 475
BstMWI GCNNNNNNNGC 2 cut(s) 35, 139
BstNSI RCATGY 1 cut(s) 596
BstV1I GCAGC 3 cut(s) 13, 217, 587
BstV2I GAAGAC 1 cut(s) 395
BstXI CCANNNNNNTGG 1 cut(s) 440
Bsu36I CCTNAGG 1 cut(s) 281
BsuI GTATCC 1 cut(s) 747
BsuRI GGCC 3 cut(s) 173, 432, 717
BtgI CCRYGG 1 cut(s) 652
BtgZI GCGATG 1 cut(s) 791
BtsCI GGATG 2 cut(s) 323, 338
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 449
CseI GACGC 1 cut(s) 650
Csp6I GTAC 2 cut(s) 376, 745
CviAII CATG 8 cut(s) 68, 127, 221, 251, 463, 593, 620, 653
CviQI GTAC 2 cut(s) 376, 745
DdeI CTNAG 4 cut(s) 269, 281, 485, 517
EaeI YGGCCR 2 cut(s) 171, 430
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EciI GGCGGA 1 cut(s) 726
Ecl136II GAGCTC 2 cut(s) 256, 268
Eco130I CCWWGG 1 cut(s) 652
Eco24I GRGCYC 3 cut(s) 258, 270, 277
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 1 cut(s) 449
Eco53kI GAGCTC 2 cut(s) 256, 268
Eco81I CCTNAGG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 829
EcoICRI GAGCTC 2 cut(s) 256, 268
EcoT14I CCWWGG 1 cut(s) 652
EcoT22I ATGCAT 1 cut(s) 252
EcoT38I GRGCYC 3 cut(s) 258, 270, 277
ErhI CCWWGG 1 cut(s) 652
FaeI CATG 8 cut(s) 71, 130, 224, 254, 466, 596, 623, 656
FaqI GGGAC 1 cut(s) 634
FatI CATG 8 cut(s) 67, 126, 220, 250, 462, 592, 619, 652
FauI CCCGC 1 cut(s) 63
Fnu4HI GCNGC 3 cut(s) 27, 231, 601
FokI GGATG 2 cut(s) 310, 325
FriOI GRGCYC 3 cut(s) 258, 270, 277
Fsp4HI GCNGC 3 cut(s) 27, 231, 601
FspBI CTAG 1 cut(s) 728
GluI GCNGC 3 cut(s) 27, 231, 601
GsuI CTGGAG 1 cut(s) 126
HaeIII GGCC 3 cut(s) 173, 432, 717
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 650
Hin1II CATG 8 cut(s) 71, 130, 224, 254, 466, 596, 623, 656
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 4 cut(s) 108, 165, 799, 820
HpaII CCGG 1 cut(s) 156
HphI GGTGA 2 cut(s) 196, 269
Hpy166II GTNNAC 2 cut(s) 204, 376
Hpy188I TCNGA 2 cut(s) 246, 343
Hpy188III TCNNGA 5 cut(s) 105, 312, 359, 740, 796
Hpy8I GTNNAC 2 cut(s) 204, 376
Hpy99I CGWCG 1 cut(s) 64
HpyCH4IV ACGT 4 cut(s) 14, 62, 176, 734
HpyCH4V TGCA 6 cut(s) 29, 197, 250, 460, 509, 723
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 139
HpyF3I CTNAG 4 cut(s) 269, 281, 485, 517
HpySE526I ACGT 4 cut(s) 14, 62, 176, 734
Hsp92II CATG 8 cut(s) 71, 130, 224, 254, 466, 596, 623, 656
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 2 cut(s) 130, 320
Lsp1109I GCAGC 3 cut(s) 13, 217, 587
LweI GCATC 2 cut(s) 237, 332
MaeI CTAG 1 cut(s) 728
MaeII ACGT 4 cut(s) 14, 62, 176, 734
MaeIII GTNAC 2 cut(s) 10, 547
MboII GAAGA 6 cut(s) 250, 400, 425, 491, 641, 820
MfeI CAATTG 1 cut(s) 379
MhlI GDGCHC 3 cut(s) 258, 270, 277
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 2 cut(s) 379, 839
MluNI TGGCCA 1 cut(s) 173
MlyI GAGTC 3 cut(s) 117, 174, 814
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 6 cut(s) 76, 80, 144, 290, 524, 707
Mox20I TGGCCA 1 cut(s) 173
Mph1103I ATGCAT 1 cut(s) 252
MscI TGGCCA 1 cut(s) 173
MseI TTAA 4 cut(s) 288, 476, 750, 842
MslI CAYNNNNRTG 1 cut(s) 225
Msp20I TGGCCA 1 cut(s) 173
MspA1I CMGCKG 2 cut(s) 330, 600
MspI CCGG 1 cut(s) 156
MunI CAATTG 1 cut(s) 379
MwoI GCNNNNNNNGC 2 cut(s) 35, 139
NcoI CCATGG 1 cut(s) 652
NlaIII CATG 8 cut(s) 71, 130, 224, 254, 466, 596, 623, 656
NlaIV GGNNCC 1 cut(s) 132
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 547
NsiI ATGCAT 1 cut(s) 252
NspI RCATGY 1 cut(s) 596
PciSI GCTCTTC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 803
PfeI GAWTC 1 cut(s) 799
PflFI GACNNNGTC 1 cut(s) 638
PflMI CCANNNNNTGG 1 cut(s) 180
PkrI GCNGC 3 cut(s) 28, 232, 602
PleI GAGTC 3 cut(s) 116, 173, 814
PpsI GAGTC 3 cut(s) 116, 173, 814
Psp124BI GAGCTC 2 cut(s) 258, 270
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 1 cut(s) 449
PsyI GACNNNGTC 1 cut(s) 638
PvuII CAGCTG 1 cut(s) 600
RsaI GTAC 2 cut(s) 377, 746
RsaNI GTAC 2 cut(s) 376, 745
RseI CAYNNNNRTG 1 cut(s) 225
SacI GAGCTC 2 cut(s) 258, 270
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 4 cut(s) 288, 476, 750, 842
SatI GCNGC 3 cut(s) 27, 231, 601
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 3 cut(s) 117, 174, 814
SduI GDGCHC 3 cut(s) 258, 270, 277
SfaNI GCATC 2 cut(s) 237, 332
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 2 cut(s) 143, 859
SmoI CTYRAG 2 cut(s) 143, 859
Sse9I AATT 2 cut(s) 379, 839
SsiI CCGC 4 cut(s) 56, 330, 447, 711
SspI AATATT 1 cut(s) 292
SspMI CTAG 1 cut(s) 728
SstI GAGCTC 2 cut(s) 258, 270
StyI CCWWGG 1 cut(s) 652
TaiI ACGT 4 cut(s) 17, 65, 179, 737
TaqI TCGA 5 cut(s) 74, 561, 642, 797, 806
TaqII GACCGA 1 cut(s) 407
TasI AATT 2 cut(s) 379, 839
TatI WGTACW 2 cut(s) 375, 744
TfiI GAWTC 1 cut(s) 799
Tru1I TTAA 4 cut(s) 288, 476, 750, 842
Tru9I TTAA 4 cut(s) 288, 476, 750, 842
TscAI CASTG 1 cut(s) 462
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 3 cut(s) 26, 230, 600
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 250, 491, 757
TspRI CASTG 1 cut(s) 462
Tth111I GACNNNGTC 1 cut(s) 638
Van91I CCANNNNNTGG 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 449
XceI RCATGY 1 cut(s) 596
XspI CTAG 1 cut(s) 728
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.