AT2G25350

phox (PX) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005258
Physical Location & Seq
Forward (+)
10797353 .. 10801058
3706 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25350.2

Sequence Viewer

Length: 1932 bp
ATGCAAAGACGGAGTCCACCGAAACATAGACACGATGGAGCATCGCCACTTCCACTGGGAATGGATTGGAGCCCTCCACCGCGTAATTGGAATGGTAGAGATACAATTTGGCCTCATGATTTTCGAACTGGATGGAGTTATTGTGTCACTATACCATCGTGGACACTTCTTTCAAAATCGAAAAATTCAGATCCTATAGTGTTTTACAGGGTTCAGGTAAGTGTGCAATCTCCAGAAGGTGTTAGCACAATGCGAGGAATTCTGAGGAGATTTAATGATTTTGTAAAGCTTTTAGCTGATCTCAAAAGAGCATTTCCTAGGAAAAGTTTTCCATCAGCTCCTCCTAAAGGATTTTTGCGTGTCAAAAGTAGAGATATGTTAGAAGAGAGAAGGTGCTCTCTGGAGGATTGGATGACGAAGTTGTTATCTGATATAGAATTGGCAAGAAGTGTTGTAGTTGCATCCTTCCTCGAACTAGAAGCTACTGCTAGGTCTGCATGCCAAGTTGTAGATCAGAATGCTTCCGATTCCAATGATGATGGAAATAGCACAAGCTTGTCGTCTCTAGTTCACCCAAATTCTGGTGGCAGTTCTTTGCTCTCATCTGACTATGGCAGCGATACTGCTTATGAAACATCTGAGCTCGGATCAGCAAGTTTAGGTCAGGATGATGTTTCTGAAACTGATACTGGAGATCTAACTTTGGATGAAGATCTGACAAATCCAACAGAGAAGCTTGTAAAGTTTAGCATGTCCAACATTGACGAGGGACTGAGTATGAGTCAAACTATTATCGAGCAGCTAGAAGATTTTCCTAAGCACAGAGTCCATTTAGGATATGCCAATGATATCACAGAGACGAATTCGTACAATGGAAAAGCTTCTAAAGGTGTATTTCGAGCAAACAACGACTTACGGTGTCGCTCTGAATCAGAAACTAGTCACTCAGTGATGCATGATCGAAAGCTTTCTCTTGAGAGCGCTGACGGGGTTTCGTTACTAGCCGGTGAGACATCAACCTCTAGTATCTTGAGCTCCATTGTACATAGCCAGCTGGATGTTAATCATGATATATCTGTTGGTAACCTGGAGATACCGGGTAATGGACGAATAGTTCTCCCGCTGAAAATGCATAGTAAATTAAATAGGATTCTATTAACTATGAATGAGAGACTTCTCAATTCAAAAACAGACATGGAGGATCTTATAGCGAGACTAAATCAAGAGACAGCGGTGAAGGAATATCTGAACAGAAAGGTTGATGATCTGGAAGTGGAACTTGAAACTACTAAACAGAGAAACAAAGAGAATTTGGAGCAAGCTTTGATGACCGAGAGACAGAGTGTTACCAAGATGCAGTGGGATATGGAAGAACTCAGGCAAAAAACTTTTGAGATGGAACTGAAGTTAAAGTCAAAAGAGGATGGGAGTTCAGATTCTAAGACGTCAGGAAACTCAACAATCTCTGAGAGCCATGAGCTGTTACAAGAAATGGATGCCACAAAACAGCAACTGGAAGATCTTTCTAGACGGTATGTTGAACTGGAGGCAAAATCAAAGGCAGACATAAAAGTGCTTGTCAGAGAGGTCAAATCCCTGAGACGTTCACATATGGAAATGGAAAAGGAACTGACTCGATCGCTGACAGAGAAGTCTGACACTGAGAAACTCCTCCAACAGGAAAGGATAATAGTCGAGAACACGCTAGAAGCAAGAAGAAGGCTGTATAGTGACTGTGAAATTCTCCACGACAGGCTTAAAGTGAACAACACAAACTTGTCAATGGATGAGTCCAGTAACAACAGAGAAGACTTATCTGAAGTATCAAATGCCTTGCAAGATCAGATTGAGGCGCAACTGCTGTTAGGGTTTGATGAGACTGCTTCTGAGGATGAGCTGAGGAAGATAATGGCAGATATGTATGAGGACTGA

Protein Analysis

643

Amino Acids

72.82

Weight (kDa)

5.09

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PX PF00787 85 - 158 7.7e-14 PX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1651
AatII GACGTC 1 cut(s) 1448
AccB7I CCANNNNNTGG 1 cut(s) 581
AccII CGCG 1 cut(s) 82
AciI CCGC 3 cut(s) 80, 1121, 1232
AclWI GGATC 3 cut(s) 185, 655, 1209
AcsI RAATTY 6 cut(s) 184, 258, 577, 862, 1309, 1740
AcuI CTGAAG 2 cut(s) 1424, 1839
AcyI GRCGYC 1 cut(s) 1445
AdeI CACNNNGTG 1 cut(s) 949
AfaI GTAC 2 cut(s) 869, 1044
AfeI AGCGCT 1 cut(s) 982
AfiI CCNNNNNNNGG 4 cut(s) 347, 581, 1103, 1678
AgsI TTSAA 4 cut(s) 174, 1185, 1283, 1541
AhlI ACTAGT 1 cut(s) 938
AjnI CCWGG 1 cut(s) 1086
AloI GAACNNNNNNTCC 2 cut(s) 1098, 1130
Alw21I GWGCWC 3 cut(s) 398, 645, 1037
Alw26I GTCTC 9 cut(s) 567, 851, 1004, 1165, 1207, 1220, 1330, 1594, 1871
AlwI GGATC 3 cut(s) 185, 655, 1209
AlwNI CAGNNNCTG 1 cut(s) 1513
Aor51HI AGCGCT 1 cut(s) 982
AoxI GGCC 1 cut(s) 110
ApeKI GCWGC 2 cut(s) 615, 799
ApoI RAATTY 6 cut(s) 184, 258, 577, 862, 1309, 1740
ArsI GACNNNNNNTTYG 1 cut(s) 30
Asp700I GAANNNNTTC 3 cut(s) 810, 880, 967
AspA2I CCTAGG 1 cut(s) 317
AspLEI GCGC 2 cut(s) 983, 1855
AsuC2I CCSGG 1 cut(s) 1098
AsuHPI GGTGA 3 cut(s) 563, 1019, 1246
AsuII TTCGAA 1 cut(s) 124
AvrII CCTAGG 1 cut(s) 317
BanII GRGCYC 3 cut(s) 74, 645, 1037
BbsI GAAGAC 1 cut(s) 1815
Bbv12I GWGCWC 3 cut(s) 398, 645, 1037
BbvCI CCTCAGC 1 cut(s) 1898
BbvI GCAGC 2 cut(s) 627, 811
BccI CCATC 7 cut(s) 29, 126, 163, 340, 533, 1390, 1418
BciT130I CCWGG 1 cut(s) 1088
BcnI CCSGG 1 cut(s) 1098
BcoDI GTCTC 9 cut(s) 567, 851, 1004, 1165, 1207, 1220, 1330, 1594, 1871
BcuI ACTAGT 1 cut(s) 938
BfmI CTRYAG 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 984
BglII AGATCT 3 cut(s) 694, 712, 1519
BisI GCNGC 2 cut(s) 616, 800
BlnI CCTAGG 1 cut(s) 317
BlsI GCNGC 2 cut(s) 617, 801
Bme1390I CCNGG 2 cut(s) 1088, 1098
BmiI GGNNCC 1 cut(s) 71
BmrFI CCNGG 2 cut(s) 1088, 1098
BmrI ACTGGG 1 cut(s) 65
BmsI GCATC 5 cut(s) 50, 470, 942, 1344, 1486
BmuI ACTGGG 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 1815
BpmI CTGGAG 5 cut(s) 216, 422, 711, 1109, 1565
Bpu10I CCTNAGC 2 cut(s) 816, 1898
Bpu14I TTCGAA 1 cut(s) 124
BpuEI CTTGAG 2 cut(s) 995, 1051
BpuMI CCSGG 1 cut(s) 1098
BsaBI GATNNNNATC 2 cut(s) 711, 1062
BsaHI GRCGYC 1 cut(s) 1445
BsaJI CCNNGG 1 cut(s) 317
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 4 cut(s) 347, 581, 1103, 1678
Bse118I RCCGGY 1 cut(s) 1004
Bse1I ACTGG 6 cut(s) 60, 133, 694, 1518, 1548, 1794
Bse8I GATNNNNATC 2 cut(s) 711, 1062
BseBI CCWGG 1 cut(s) 1088
BseDI CCNNGG 1 cut(s) 317
BseJI GATNNNNATC 2 cut(s) 711, 1062
BseLI CCNNNNNNNGG 4 cut(s) 347, 581, 1103, 1678
BseNI ACTGG 6 cut(s) 60, 133, 694, 1518, 1548, 1794
BseRI GAGGAG 3 cut(s) 280, 330, 1661
BseXI GCAGC 2 cut(s) 627, 811
Bsh1236I CGCG 1 cut(s) 82
Bsh1285I CGRYCG 1 cut(s) 1640
BshFI GGCC 1 cut(s) 112
BsiEI CGRYCG 1 cut(s) 1640
BsiHKAI GWGCWC 3 cut(s) 398, 645, 1037
BsiSI CCGG 2 cut(s) 1005, 1097
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 4 cut(s) 347, 581, 1103, 1678
BsmAI GTCTC 9 cut(s) 567, 851, 1004, 1165, 1207, 1220, 1330, 1594, 1871
BsmBI CGTCTC 3 cut(s) 567, 851, 1594
BsmFI GGGAC 1 cut(s) 783
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 1 cut(s) 112
Bsp119I TTCGAA 1 cut(s) 124
Bsp1286I GDGCHC 4 cut(s) 74, 398, 645, 1037
Bsp1407I TGTACA 1 cut(s) 1042
BspACI CCGC 3 cut(s) 80, 1121, 1232
BspANI GGCC 1 cut(s) 112
BspFNI CGCG 1 cut(s) 82
BspHI TCATGA 2 cut(s) 115, 1066
BspLI GGNNCC 1 cut(s) 71
BspPI GGATC 3 cut(s) 185, 655, 1209
BspT104I TTCGAA 1 cut(s) 124
BsrFI RCCGGY 1 cut(s) 1004
BsrGI TGTACA 1 cut(s) 1042
BsrI ACTGG 6 cut(s) 60, 133, 694, 1518, 1548, 1794
BssAI RCCGGY 1 cut(s) 1004
BssECI CCNNGG 1 cut(s) 317
BssNI GRCGYC 1 cut(s) 1445
BssT1I CCWWGG 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 1088
Bst4CI ACNGT 3 cut(s) 918, 1533, 1736
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 1445
BstAUI TGTACA 1 cut(s) 1042
BstBI TTCGAA 1 cut(s) 124
BstC8I GCNNGC 3 cut(s) 499, 1052, 1320
BstEII GGTNACC 1 cut(s) 1082
BstENI CCTNNNNNAGG 2 cut(s) 345, 1676
BstFNI CGCG 1 cut(s) 82
BstH2I RGCGCY 1 cut(s) 984
BstHHI GCGC 2 cut(s) 983, 1855
BstMAI GTCTC 9 cut(s) 567, 851, 1004, 1165, 1207, 1220, 1330, 1594, 1871
BstMCI CGRYCG 1 cut(s) 1640
BstMWI GCNNNNNNNGC 2 cut(s) 494, 1129
BstNI CCWGG 1 cut(s) 1088
BstNSI RCATGY 2 cut(s) 501, 754
BstPI GGTNACC 1 cut(s) 1082
BstSCI CCNGG 2 cut(s) 1086, 1096
BstSFI CTRYAG 1 cut(s) 195
BstUI CGCG 1 cut(s) 82
BstV1I GCAGC 2 cut(s) 627, 811
BstV2I GAAGAC 1 cut(s) 1815
BstX2I RGATCY 5 cut(s) 190, 694, 712, 1201, 1519
BstYI RGATCY 5 cut(s) 190, 694, 712, 1201, 1519
BsuRI GGCC 1 cut(s) 112
BtgZI GCGATG 1 cut(s) 27
BtsI GCAGTG 1 cut(s) 1364
BtsIMutI CAGTG 4 cut(s) 53, 954, 1364, 1659
Cac8I GCNNGC 3 cut(s) 499, 1052, 1320
CaiI CAGNNNCTG 1 cut(s) 1513
CciI TCATGA 2 cut(s) 115, 1066
CfoI GCGC 2 cut(s) 983, 1855
Cfr10I RCCGGY 1 cut(s) 1004
Csp6I GTAC 2 cut(s) 868, 1043
CviAII CATG 7 cut(s) 116, 498, 751, 956, 1067, 1195, 1475
CviQI GTAC 2 cut(s) 868, 1043
DraIII CACNNNGTG 1 cut(s) 949
DrdI GACNNNNNNGTC 1 cut(s) 1651
DseDI GACNNNNNNGTC 1 cut(s) 1651
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Ecl136II GAGCTC 2 cut(s) 643, 1035
Eco130I CCWWGG 1 cut(s) 317
Eco24I GRGCYC 3 cut(s) 74, 645, 1037
Eco32I GATATC 1 cut(s) 850
Eco47III AGCGCT 1 cut(s) 982
Eco53kI GAGCTC 2 cut(s) 643, 1035
Eco57I CTGAAG 2 cut(s) 1424, 1839
Eco91I GGTNACC 1 cut(s) 1082
EcoICRI GAGCTC 2 cut(s) 643, 1035
EcoNI CCTNNNNNAGG 2 cut(s) 345, 1676
EcoO65I GGTNACC 1 cut(s) 1082
EcoRI GAATTC 2 cut(s) 258, 862
EcoRII CCWGG 1 cut(s) 1086
EcoRV GATATC 1 cut(s) 850
EcoT14I CCWWGG 1 cut(s) 317
EcoT22I ATGCAT 2 cut(s) 957, 1134
EcoT38I GRGCYC 3 cut(s) 74, 645, 1037
ErhI CCWWGG 1 cut(s) 317
Esp3I CGTCTC 3 cut(s) 567, 851, 1594
FaeI CATG 7 cut(s) 119, 501, 754, 959, 1070, 1198, 1478
FalI AAGNNNNNCTT 2 cut(s) 1263, 1295
FaqI GGGAC 1 cut(s) 783
FatI CATG 7 cut(s) 115, 497, 750, 955, 1066, 1194, 1474
FauI CCCGC 1 cut(s) 1128
FauNDI CATATG 1 cut(s) 1611
Fnu4HI GCNGC 2 cut(s) 616, 800
FriOI GRGCYC 3 cut(s) 74, 645, 1037
Fsp4HI GCNGC 2 cut(s) 616, 800
GlaI GCGC 2 cut(s) 982, 1854
GluI GCNGC 2 cut(s) 616, 800
GsuI CTGGAG 5 cut(s) 216, 422, 711, 1109, 1565
HaeII RGCGCY 1 cut(s) 984
HaeIII GGCC 1 cut(s) 112
HapII CCGG 2 cut(s) 1005, 1097
HhaI GCGC 2 cut(s) 983, 1855
Hin1I GRCGYC 1 cut(s) 1445
Hin1II CATG 7 cut(s) 119, 501, 754, 959, 1070, 1198, 1478
Hin6I GCGC 2 cut(s) 981, 1853
HinP1I GCGC 2 cut(s) 981, 1853
HindIII AAGCTT 6 cut(s) 287, 553, 734, 879, 965, 1320
HinfI GANTC 9 cut(s) 13, 527, 781, 825, 929, 1150, 1436, 1633, 1790
HpaII CCGG 2 cut(s) 1005, 1097
HphI GGTGA 3 cut(s) 563, 1019, 1246
Hpy166II GTNNAC 5 cut(s) 17, 162, 571, 1607, 1765
Hpy8I GTNNAC 5 cut(s) 17, 162, 571, 1607, 1765
HpyAV CCTTC 5 cut(s) 230, 384, 475, 1231, 1713
HpyCH4III ACNGT 3 cut(s) 918, 1533, 1736
HpyCH4IV ACGT 2 cut(s) 1445, 1603
HpyCH4V TGCA 8 cut(s) 4, 226, 461, 497, 955, 1132, 1357, 1837
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 1129
HpySE526I ACGT 2 cut(s) 1445, 1603
Hsp92I GRCGYC 1 cut(s) 1445
Hsp92II CATG 7 cut(s) 119, 501, 754, 959, 1070, 1198, 1478
HspAI GCGC 2 cut(s) 981, 1853
LmnI GCTCC 5 cut(s) 38, 69, 343, 1040, 1315
Lsp1109I GCAGC 2 cut(s) 627, 811
LweI GCATC 5 cut(s) 50, 470, 942, 1344, 1486
MaeII ACGT 2 cut(s) 1445, 1603
MaeIII GTNAC 8 cut(s) 145, 941, 996, 1082, 1345, 1482, 1730, 1796
MboII GAAGA 8 cut(s) 395, 722, 818, 1382, 1529, 1728, 1820, 1915
MflI RGATCY 5 cut(s) 190, 694, 712, 1201, 1519
MhlI GDGCHC 4 cut(s) 74, 398, 645, 1037
MlyI GAGTC 5 cut(s) 22, 790, 834, 1627, 1799
MmeI TCCRAC 3 cut(s) 749, 780, 1699
Mph1103I ATGCAT 2 cut(s) 957, 1134
MroXI GAANNNNTTC 3 cut(s) 810, 880, 967
MseI TTAA 6 cut(s) 273, 1062, 1142, 1157, 1409, 1758
MslI CAYNNNNRTG 1 cut(s) 1571
MspA1I CMGCKG 3 cut(s) 1054, 1123, 1232
MspI CCGG 2 cut(s) 1005, 1097
MspR9I CCNGG 2 cut(s) 1088, 1098
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 1 cut(s) 1088
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 2 cut(s) 494, 1129
NciI CCSGG 1 cut(s) 1098
NdeI CATATG 1 cut(s) 1611
NlaIII CATG 7 cut(s) 119, 501, 754, 959, 1070, 1198, 1478
NlaIV GGNNCC 1 cut(s) 71
NmuCI GTSAC 3 cut(s) 145, 941, 1730
NsiI ATGCAT 2 cut(s) 957, 1134
NspI RCATGY 2 cut(s) 501, 754
NspV TTCGAA 1 cut(s) 124
PaeI GCATGC 1 cut(s) 501
PagI TCATGA 2 cut(s) 115, 1066
PctI GAATGC 1 cut(s) 523
PdmI GAANNNNTTC 3 cut(s) 810, 880, 967
PfeI GAWTC 4 cut(s) 527, 929, 1150, 1436
PflFI GACNNNGTC 1 cut(s) 12
PflMI CCANNNNNTGG 1 cut(s) 581
PkrI GCNGC 2 cut(s) 617, 801
Ple19I CGATCG 1 cut(s) 1640
PleI GAGTC 5 cut(s) 21, 789, 833, 1627, 1798
PpsI GAGTC 5 cut(s) 21, 789, 833, 1627, 1798
Psp124BI GAGCTC 2 cut(s) 645, 1037
Psp6I CCWGG 1 cut(s) 1086
PspEI GGTNACC 1 cut(s) 1082
PspGI CCWGG 1 cut(s) 1086
PspN4I GGNNCC 1 cut(s) 71
PstNI CAGNNNCTG 1 cut(s) 1513
PsuI RGATCY 5 cut(s) 190, 694, 712, 1201, 1519
PsyI GACNNNGTC 1 cut(s) 12
PvuI CGATCG 1 cut(s) 1640
PvuII CAGCTG 1 cut(s) 1054
RsaI GTAC 2 cut(s) 869, 1044
RsaNI GTAC 2 cut(s) 868, 1043
RseI CAYNNNNRTG 1 cut(s) 1571
SacI GAGCTC 2 cut(s) 645, 1037
SaqAI TTAA 6 cut(s) 273, 1062, 1142, 1157, 1409, 1758
SatI GCNGC 2 cut(s) 616, 800
SchI GAGTC 5 cut(s) 22, 790, 834, 1627, 1799
ScrFI CCNGG 2 cut(s) 1088, 1098
SduI GDGCHC 4 cut(s) 74, 398, 645, 1037
SfaNI GCATC 5 cut(s) 50, 470, 942, 1344, 1486
SfcI CTRYAG 1 cut(s) 195
SfuI TTCGAA 1 cut(s) 124
SmiMI CAYNNNNRTG 1 cut(s) 1571
SmlI CTYRAG 2 cut(s) 974, 1030
SmoI CTYRAG 2 cut(s) 974, 1030
SpeI ACTAGT 1 cut(s) 938
SphI GCATGC 1 cut(s) 501
SsiI CCGC 3 cut(s) 80, 1121, 1232
SstI GAGCTC 2 cut(s) 645, 1037
StyD4I CCNGG 2 cut(s) 1086, 1096
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 3 cut(s) 918, 1533, 1736
TaiI ACGT 2 cut(s) 1448, 1606
TaqI TCGA 8 cut(s) 124, 179, 471, 795, 898, 961, 1636, 1695
TaqII GACCGA 1 cut(s) 1346
TatI WGTACW 1 cut(s) 1042
TfiI GAWTC 4 cut(s) 527, 929, 1150, 1436
Tru1I TTAA 6 cut(s) 273, 1062, 1142, 1157, 1409, 1758
Tru9I TTAA 6 cut(s) 273, 1062, 1142, 1157, 1409, 1758
TscAI CASTG 4 cut(s) 60, 954, 1364, 1666
TseFI GTSAC 3 cut(s) 145, 941, 1730
TseI GCWGC 2 cut(s) 615, 799
Tsp45I GTSAC 3 cut(s) 145, 941, 1730
TspDTI ATGAA 3 cut(s) 645, 723, 1178
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 4 cut(s) 60, 954, 1364, 1666
Tth111I GACNNNGTC 1 cut(s) 12
Van91I CCANNNNNTGG 1 cut(s) 581
XagI CCTNNNNNAGG 2 cut(s) 345, 1676
XapI RAATTY 6 cut(s) 184, 258, 577, 862, 1309, 1740
XbaI TCTAGA 1 cut(s) 1526
XceI RCATGY 2 cut(s) 501, 754
XcmI CCANNNNNNNNNTGG 1 cut(s) 84
XmaJI CCTAGG 1 cut(s) 317
XmnI GAANNNNTTC 3 cut(s) 810, 880, 967
ZraI GACGTC 1 cut(s) 1446
Zsp2I ATGCAT 2 cut(s) 957, 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.