Rh6CG400000

PhoX homologous domain, present in p47phox and p40phox.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0005258
Physical Location & Seq
Forward (+)
58704323 .. 58709769
5447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG400000.1

Sequence Viewer

Length: 2214 bp
ATGCAGCAGAGACGGAGTCCTCCGAAGCACAGACACGATGGGACTTCGCCGCTGCCTCTTGGCATGGATTGGAGTCCTCCTCCCCGAAAATGGAACGGGAGGGACACAGTTTGGCCGCACGATCCTCACACAGGATGGAGTTATTGTGTCACCATTCCTTCCTGGGTTGTTCTTCCCAAATCAAGAAATTCAGACCCCGTTGTGTTCTACAGGGTTCAGGTGGGACTGCAATCACCAGATGGTATTACAACTACAAGTGGAGTGCTAAGAAGATTTAATGATTTTCTGAAGTTATTTAATGACCTTAAAAAGGCATTTCCCAAGAAAAATCTCCCACCAACTCCACCCAAGGGACTTCTACGGATGAAGAGTCAAGAATTGTTGGAAGAGAGAAGGTGCTCTTTGGAGGCCTGGATGATAAAGCTGCTATCTGATATAGATATATCAAGAAGCGTTGCAGTGGCATCCTTTCTTGAATTAGAATCTGCAGCTAGGTCCTCATTCCAAGACACAAGTCAACACAATTCAGAAGCGAATTCACTTCAGTCGCCTTCCCATCCTACCTTACCTGCCATTCTTGGTAGCTCGTCCATTACATCAGATTATGGAAGTGATACTGCTTATGAGACATCTGACCTGGGAACACCAAGGCTAGGAAGGGATGATAGTTCAGAACTGGGTATCGAAGATCTAGCATTGGATGAAGATCTAACAAGTCCAATAGAAAAGCTTGTGAAGTTTGGCATGACCAATATTGATGAGGGACTGTTTATGGGGCAGACTATACTAGAGCAACTGGAAGGCCTTCCTAAACATAAAATGCATGGGAGACATGTCAATAATGTTACAGGGAAGGACCTGTATAATGGAAGTGCTTCTAAACCTTCAATACTAGCTCGTAATGGAATGGAACTTTTCTCTCAGCCAGAGCATAGTAAAGTTTTTAGTCATGCTCGCAAGCTCTCAGATGAAAGTGTGGGAAGTGATGTAAGTTCTCTAAGAGGTAGTGATATGTCTAACTCTGGGGTTCCAAATTCAATTGGTGATGGCACCCTCGACCTTCTTGGAGGTGCTGATGTTTCAAGGATCATGGAAATGCTTGGAAGTACAGAATTACAAACTTCAGGCAGCACTCAAGTAGTTCTTCCATTGGATCAGCGTCCTAAACTGAATAGGGTTATGTTGACCATGCAGCAAAGACTAGTCACAGCTAAAACAGACATGGAAGATCTTATAGCGAGATTAAATCAAGAAATAGCAGTCAAAGATTACCTCTCAACAAAGGTCAAGGATTTGGAAGTGGAACTTGAAACTACTAGACAGAAGACTAAGGAAAACCTTCAGCAAGCTATTTTGGTTGAAAGGGAAAGGTTTACCAAAATGCAGTGGGATATGGAGGAACTTCGTCGGAAGTCATTGGAAATGGAGTTGAAGATGAAGTCTGAACAGGATAACACGTCATGCCCAGAGGCAAAAGATGATTCCACTATTCAAGAGAAGGACATATCTCCACCAGAGTTAGATTCTTCTAAGGAGCAGCTTGAGAATTTGTCAAAGCGATATGAAGAGCTAGAAGCAAAATCAAGAGCAGATATTAAAGTTCTTGTTAAAGAAGTTAAATCCTTGCGTAGTTCCCAAGCAGAACTCAGGCATGAGCTTAGTAAATCTCTGAAGGAGAAATCTGAATCTGAGAAGCTTCTCCAACGGGAACAACAGAGGAGCAAACACGCAGAAACTGCTAGAGAGAAATTGTTGCAGGAATGCAAGATTCTTCACAAACAGCTTCAGGAGTGCAATACCAATTTTCCTTCTGAAGGTGAAGATAACTTCGTTTTGGGTTCATCGATACAAGAGGCTTCAGATTTGTTGACAACATCAGACAACCGAATTAACCAACTACTTTCAGAGGTACGTTTTGTTTCTTTCCTTGTGCCTGTGACCTCTTATTTTTTGCCTTCTGCTCTCATCATTCAACACGTTGTTCAGGCACAACTCTTGGAACAAGACAACAGAGATGATGTTTCTGACGTTGGTGAAGCTGACGATGTTACAGATGATACAAGGACAACAGACGACGAGTTGAGGAAGATGCTCGTAGACATTTTTACTGAGAATGCGAGCTTGCGGAAACAGGTGAACTCTGTTATACGACACGCTCTCAAAGTGGACATCCAGTGTAAGAAAGACGATGTCCCCAACGACAAACTTCGCTAG

Protein Analysis

737

Amino Acids

83.06

Weight (kDa)

5.45

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PX PF00787 85 - 159 1.8e-13 PX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 577
AccB1I GGYRCC 1 cut(s) 1049
AccI GTMKAC 1 cut(s) 2097
AciI CCGC 3 cut(s) 50, 116, 2125
AclWI GGATC 3 cut(s) 116, 1094, 1161
AcoI YGGCCR 1 cut(s) 113
AcsI RAATTY 4 cut(s) 187, 535, 1033, 1546
AcuI CTGAAG 8 cut(s) 308, 527, 1107, 1325, 1691, 1769, 1833, 1842
AfaI GTAC 2 cut(s) 1108, 1911
AfiI CCNNNNNNNGG 6 cut(s) 90, 131, 310, 350, 653, 1814
AflIII ACRYGT 3 cut(s) 832, 1455, 1975
AgsI TTSAA 9 cut(s) 476, 888, 1038, 1083, 1310, 1361, 1432, 1493, 1973
AhlI ACTAGT 1 cut(s) 1201
AjiI CACGTC 1 cut(s) 1458
AjnI CCWGG 3 cut(s) 161, 410, 636
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 3 cut(s) 4, 620, 823
AlwI GGATC 3 cut(s) 116, 1094, 1161
AlwNI CAGNNNCTG 1 cut(s) 1736
AoxI GGCC 3 cut(s) 113, 408, 802
ApeKI GCWGC 7 cut(s) 4, 52, 424, 488, 1128, 1192, 1537
ApoI RAATTY 4 cut(s) 187, 535, 1033, 1546
Asp700I GAANNNNTTC 3 cut(s) 804, 874, 1338
AspS9I GGNCC 2 cut(s) 495, 856
AsuHPI GGTGA 6 cut(s) 142, 225, 1055, 1829, 2045, 2146
AvaII GGWCC 2 cut(s) 495, 856
BanI GGYRCC 1 cut(s) 1049
BbsI GAAGAC 1 cut(s) 1331
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 7 cut(s) 16, 39, 411, 500, 1140, 1204, 1549
BccI CCATC 5 cut(s) 32, 129, 233, 564, 1040
BciT130I CCWGG 3 cut(s) 163, 412, 638
BcoDI GTCTC 3 cut(s) 4, 620, 823
BcuI ACTAGT 1 cut(s) 1201
BfmI CTRYAG 2 cut(s) 208, 486
BfuAI ACCTGC 1 cut(s) 577
BglII AGATCT 3 cut(s) 688, 706, 1228
BisI GCNGC 9 cut(s) 5, 50, 53, 116, 425, 489, 1129, 1193, 1538
BlsI GCNGC 9 cut(s) 6, 51, 54, 117, 426, 490, 1130, 1194, 1539
Bme1390I CCNGG 3 cut(s) 163, 412, 638
Bme18I GGWCC 2 cut(s) 495, 856
BmgBI CACGTC 1 cut(s) 1458
BmgT120I GGNCC 2 cut(s) 495, 856
BmiI GGNNCC 2 cut(s) 1029, 1051
BmrFI CCNGG 3 cut(s) 163, 412, 638
BmrI ACTGGG 1 cut(s) 686
BmsI GCATC 2 cut(s) 473, 2079
BmuI ACTGGG 1 cut(s) 686
BoxI GACNNNNGTC 1 cut(s) 513
BpiI GAAGAC 1 cut(s) 1331
BplI GAGNNNNNCTC 2 cut(s) 64, 96
BpuEI CTTGAG 2 cut(s) 1119, 1562
Bsa29I ATCGAT 1 cut(s) 1844
BsaBI GATNNNNATC 1 cut(s) 705
BsaJI CCNNGG 4 cut(s) 162, 348, 637, 647
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 6 cut(s) 90, 131, 310, 350, 653, 1814
Bse1I ACTGG 3 cut(s) 681, 801, 2173
Bse8I GATNNNNATC 1 cut(s) 705
BseBI CCWGG 3 cut(s) 163, 412, 638
BseCI ATCGAT 1 cut(s) 1844
BseDI CCNNGG 4 cut(s) 162, 348, 637, 647
BseGI GGATG 8 cut(s) 140, 369, 420, 464, 556, 667, 706, 2169
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 6 cut(s) 90, 131, 310, 350, 653, 1814
BseMII CTCAG 5 cut(s) 935, 978, 1660, 1680, 2100
BseNI ACTGG 3 cut(s) 681, 801, 2173
BseRI GAGGAG 2 cut(s) 69, 1732
BseXI GCAGC 7 cut(s) 16, 39, 411, 500, 1140, 1204, 1549
BshFI GGCC 3 cut(s) 115, 410, 804
BshNI GGYRCC 1 cut(s) 1049
BshVI ATCGAT 1 cut(s) 1844
BsiHKAI GWGCWC 1 cut(s) 401
BslFI GGGAC 6 cut(s) 55, 116, 237, 366, 777, 2177
BslI CCNNNNNNNGG 6 cut(s) 90, 131, 310, 350, 653, 1814
BsmAI GTCTC 3 cut(s) 4, 620, 823
BsmBI CGTCTC 1 cut(s) 4
BsmFI GGGAC 6 cut(s) 55, 116, 237, 366, 777, 2177
BsmI GAATGC 2 cut(s) 1766, 2119
BsnI GGCC 3 cut(s) 115, 410, 804
Bsp1286I GDGCHC 1 cut(s) 401
Bsp143I GATC 6 cut(s) 121, 688, 706, 1086, 1153, 1228
BspACI CCGC 3 cut(s) 50, 116, 2125
BspANI GGCC 3 cut(s) 115, 410, 804
BspCNI CTCAG 5 cut(s) 934, 977, 1659, 1681, 2101
BspDI ATCGAT 1 cut(s) 1844
BspLI GGNNCC 2 cut(s) 1029, 1051
BspMAI CTGCAG 1 cut(s) 490
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 3 cut(s) 116, 1094, 1161
BspQI GCTCTTC 1 cut(s) 1560
BspT107I GGYRCC 1 cut(s) 1049
BsrI ACTGG 3 cut(s) 681, 801, 2173
BssECI CCNNGG 4 cut(s) 162, 348, 637, 647
BssMI GATC 6 cut(s) 121, 688, 706, 1086, 1153, 1228
BssT1I CCWWGG 2 cut(s) 348, 647
Bst2UI CCWGG 3 cut(s) 163, 412, 638
Bst4CI ACNGT 2 cut(s) 109, 768
Bst6I CTCTTC 3 cut(s) 362, 381, 1560
BstAPI GCANNNNNTGC 1 cut(s) 1736
BstC8I GCNNGC 5 cut(s) 955, 959, 1347, 2119, 2123
BstENI CCTNNNNNAGG 3 cut(s) 129, 308, 1812
BstF5I GGATG 8 cut(s) 140, 369, 420, 464, 556, 667, 706, 2169
BstKTI GATC 6 cut(s) 124, 691, 709, 1089, 1156, 1231
BstMAI GTCTC 3 cut(s) 4, 620, 823
BstMBI GATC 6 cut(s) 121, 688, 706, 1086, 1153, 1228
BstMWI GCNNNNNNNGC 1 cut(s) 1736
BstNI CCWGG 3 cut(s) 163, 412, 638
BstNSI RCATGY 1 cut(s) 836
BstPAI GACNNNNGTC 1 cut(s) 513
BstSCI CCNGG 3 cut(s) 161, 410, 636
BstSFI CTRYAG 2 cut(s) 208, 486
BstV1I GCAGC 7 cut(s) 16, 39, 411, 500, 1140, 1204, 1549
BstV2I GAAGAC 1 cut(s) 1331
BstX2I RGATCY 3 cut(s) 688, 706, 1228
BstYI RGATCY 3 cut(s) 688, 706, 1228
Bsu15I ATCGAT 1 cut(s) 1844
BsuRI GGCC 3 cut(s) 115, 410, 804
BsuTUI ATCGAT 1 cut(s) 1844
BtrI CACGTC 1 cut(s) 1458
BtsCI GGATG 8 cut(s) 140, 369, 420, 464, 556, 667, 706, 2169
BtsI GCAGTG 2 cut(s) 465, 1391
BtsIMutI CAGTG 3 cut(s) 465, 1391, 2180
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 5 cut(s) 955, 959, 1347, 2119, 2123
CaiI CAGNNNCTG 1 cut(s) 1736
Cfr13I GGNCC 2 cut(s) 495, 856
ClaI ATCGAT 1 cut(s) 1844
CseI GACGC 1 cut(s) 1148
Csp6I GTAC 2 cut(s) 1107, 1910
CviQI GTAC 2 cut(s) 1107, 1910
DpnI GATC 6 cut(s) 123, 690, 708, 1088, 1155, 1230
DpnII GATC 6 cut(s) 121, 688, 706, 1086, 1153, 1228
EaeI YGGCCR 1 cut(s) 113
Eam1104I CTCTTC 3 cut(s) 362, 381, 1560
EarI CTCTTC 3 cut(s) 362, 381, 1560
Eco130I CCWWGG 2 cut(s) 348, 647
Eco147I AGGCCT 2 cut(s) 410, 804
Eco47I GGWCC 2 cut(s) 495, 856
Eco57I CTGAAG 8 cut(s) 308, 527, 1107, 1325, 1691, 1769, 1833, 1842
EcoNI CCTNNNNNAGG 3 cut(s) 129, 308, 1812
EcoO109I RGGNCCY 2 cut(s) 495, 856
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 3 cut(s) 161, 410, 636
EcoT14I CCWWGG 2 cut(s) 348, 647
EcoT22I ATGCAT 1 cut(s) 825
ErhI CCWWGG 2 cut(s) 348, 647
Esp3I CGTCTC 1 cut(s) 4
FalI AAGNNNNNCTT 6 cut(s) 385, 417, 1128, 1160, 1290, 1322
FaqI GGGAC 6 cut(s) 55, 116, 237, 366, 777, 2177
FblI GTMKAC 1 cut(s) 2097
Fnu4HI GCNGC 9 cut(s) 5, 50, 53, 116, 425, 489, 1129, 1193, 1538
FokI GGATG 8 cut(s) 147, 376, 427, 451, 543, 674, 713, 2156
Fsp4HI GCNGC 9 cut(s) 5, 50, 53, 116, 425, 489, 1129, 1193, 1538
GluI GCNGC 9 cut(s) 5, 50, 53, 116, 425, 489, 1129, 1193, 1538
HaeIII GGCC 3 cut(s) 115, 410, 804
HgaI GACGC 1 cut(s) 1148
HincII GTYRAC 3 cut(s) 518, 1185, 1869
HindII GTYRAC 3 cut(s) 518, 1185, 1869
HindIII AAGCTT 2 cut(s) 728, 1694
HinfI GANTC 8 cut(s) 16, 73, 370, 482, 1481, 1523, 1685, 1768
HphI GGTGA 6 cut(s) 142, 225, 1055, 1829, 2045, 2146
Hpy166II GTNNAC 7 cut(s) 518, 1185, 1374, 1869, 2098, 2137, 2167
Hpy188III TCNNGA 8 cut(s) 183, 374, 447, 473, 1250, 1493, 1584, 1787
Hpy8I GTNNAC 7 cut(s) 518, 1185, 1374, 1869, 2098, 2137, 2167
Hpy99I CGWCG 2 cut(s) 1410, 2078
HpyCH4III ACNGT 2 cut(s) 109, 768
HpyCH4IV ACGT 4 cut(s) 1457, 1912, 1977, 2028
HpyF10VI GCNNNNNNNGC 1 cut(s) 1736
HpySE526I ACGT 4 cut(s) 1457, 1912, 1977, 2028
Kzo9I GATC 6 cut(s) 121, 688, 706, 1086, 1153, 1228
LguI GCTCTTC 1 cut(s) 1560
LmnI GCTCC 2 cut(s) 1534, 1719
Lsp1109I GCAGC 7 cut(s) 16, 39, 411, 500, 1140, 1204, 1549
LweI GCATC 2 cut(s) 473, 2079
MaeII ACGT 4 cut(s) 1457, 1912, 1977, 2028
MaeIII GTNAC 5 cut(s) 148, 844, 1204, 1936, 2047
MalI GATC 6 cut(s) 123, 690, 708, 1088, 1155, 1230
MboI GATC 6 cut(s) 121, 688, 706, 1086, 1153, 1228
MfeI CAATTG 1 cut(s) 1038
MflI RGATCY 3 cut(s) 688, 706, 1228
MhlI GDGCHC 1 cut(s) 401
MlyI GAGTC 3 cut(s) 25, 82, 379
MmeI TCCRAC 3 cut(s) 363, 1388, 1726
Mph1103I ATGCAT 1 cut(s) 825
MroXI GAANNNNTTC 3 cut(s) 804, 874, 1338
MseI TTAA 8 cut(s) 276, 297, 306, 1244, 1596, 1608, 1617, 1890
MslI CAYNNNNRTG 1 cut(s) 1094
MspA1I CMGCKG 1 cut(s) 52
MspR9I CCNGG 3 cut(s) 163, 412, 638
MunI CAATTG 1 cut(s) 1038
Mva1269I GAATGC 2 cut(s) 1766, 2119
MvaI CCWGG 3 cut(s) 163, 412, 638
MwoI GCNNNNNNNGC 1 cut(s) 1736
NdeII GATC 6 cut(s) 121, 688, 706, 1086, 1153, 1228
NlaIV GGNNCC 2 cut(s) 1029, 1051
NmuCI GTSAC 3 cut(s) 148, 1204, 1936
NsiI ATGCAT 1 cut(s) 825
NspI RCATGY 1 cut(s) 836
PceI AGGCCT 2 cut(s) 410, 804
PciI ACATGT 1 cut(s) 832
PciSI GCTCTTC 1 cut(s) 1560
PctI GAATGC 2 cut(s) 1766, 2119
PdmI GAANNNNTTC 3 cut(s) 804, 874, 1338
PfeI GAWTC 5 cut(s) 482, 1481, 1523, 1685, 1768
PflFI GACNNNGTC 2 cut(s) 15, 2189
PkrI GCNGC 9 cut(s) 6, 51, 54, 117, 426, 490, 1130, 1194, 1539
PleI GAGTC 3 cut(s) 24, 81, 378
PpsI GAGTC 3 cut(s) 24, 81, 378
PpuMI RGGWCCY 2 cut(s) 495, 856
PscI ACATGT 1 cut(s) 832
PshAI GACNNNNGTC 1 cut(s) 513
Psp5II RGGWCCY 2 cut(s) 495, 856
Psp6I CCWGG 3 cut(s) 161, 410, 636
PspGI CCWGG 3 cut(s) 161, 410, 636
PspN4I GGNNCC 2 cut(s) 1029, 1051
PspPI GGNCC 2 cut(s) 495, 856
PspPPI RGGWCCY 2 cut(s) 495, 856
PstI CTGCAG 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 1736
PsuI RGATCY 3 cut(s) 688, 706, 1228
PsyI GACNNNGTC 2 cut(s) 15, 2189
RsaI GTAC 2 cut(s) 1108, 1911
RsaNI GTAC 2 cut(s) 1107, 1910
RseI CAYNNNNRTG 1 cut(s) 1094
SapI GCTCTTC 1 cut(s) 1560
SaqAI TTAA 8 cut(s) 276, 297, 306, 1244, 1596, 1608, 1617, 1890
SatI GCNGC 9 cut(s) 5, 50, 53, 116, 425, 489, 1129, 1193, 1538
Sau3AI GATC 6 cut(s) 121, 688, 706, 1086, 1153, 1228
Sau96I GGNCC 2 cut(s) 495, 856
SchI GAGTC 3 cut(s) 25, 82, 379
ScrFI CCNGG 3 cut(s) 163, 412, 638
SduI GDGCHC 1 cut(s) 401
SfaNI GCATC 2 cut(s) 473, 2079
SfcI CTRYAG 2 cut(s) 208, 486
SinI GGWCC 2 cut(s) 495, 856
SmiMI CAYNNNNRTG 1 cut(s) 1094
SmlI CTYRAG 2 cut(s) 1134, 1541
SmoI CTYRAG 2 cut(s) 1134, 1541
SpeI ACTAGT 1 cut(s) 1201
SseBI AGGCCT 2 cut(s) 410, 804
SsiI CCGC 3 cut(s) 50, 116, 2125
SspI AATATT 1 cut(s) 754
StuI AGGCCT 2 cut(s) 410, 804
StyD4I CCNGG 3 cut(s) 161, 410, 636
StyI CCWWGG 2 cut(s) 348, 647
TaaI ACNGT 2 cut(s) 109, 768
TaiI ACGT 4 cut(s) 1460, 1915, 1980, 2031
TaqI TCGA 3 cut(s) 684, 1056, 1844
TatI WGTACW 1 cut(s) 1106
TauI GCSGC 2 cut(s) 52, 118
TfiI GAWTC 5 cut(s) 482, 1481, 1523, 1685, 1768
Tru1I TTAA 8 cut(s) 276, 297, 306, 1244, 1596, 1608, 1617, 1890
Tru9I TTAA 8 cut(s) 276, 297, 306, 1244, 1596, 1608, 1617, 1890
TscAI CASTG 3 cut(s) 465, 1391, 2180
TseFI GTSAC 3 cut(s) 148, 1204, 1936
TseI GCWGC 7 cut(s) 4, 52, 424, 488, 1128, 1192, 1537
Tsp45I GTSAC 3 cut(s) 148, 1204, 1936
TspDTI ATGAA 6 cut(s) 380, 717, 984, 1451, 1578, 1830
TspGWI ACGGA 2 cut(s) 28, 376
TspRI CASTG 3 cut(s) 465, 1391, 2180
Tth111I GACNNNGTC 2 cut(s) 15, 2189
VpaK11BI GGWCC 2 cut(s) 495, 856
XagI CCTNNNNNAGG 3 cut(s) 129, 308, 1812
XapI RAATTY 4 cut(s) 187, 535, 1033, 1546
XceI RCATGY 1 cut(s) 836
XmiI GTMKAC 1 cut(s) 2097
XmnI GAANNNNTTC 3 cut(s) 804, 874, 1338
Zsp2I ATGCAT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.