AT2G25460

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009226
Physical Location & Seq
Reverse (-)
10832578 .. 10835660
3083 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25460.2

Sequence Viewer

Length: 1272 bp
ATGGCAAAGAAGGTGAGGAAGCTTCACGTGACGGTGAAGCCGGTGAGGCTTGATGGTTTACCGGCTATTCTCGGTGATGAAACGGCAGGAAAGAACCTTTCCGCGATGGTGGAGGTGAAATGGAAGGGACCTGTCTCCGGTTTTGGATTGGGCTTCGTCCCATTCTATCGTTCTAATCGACCGGTTAACCATACGAGCTCTAAACCGATTGCTTTAGGCTCAAACCACGTAGAATGGGAGGAAGAGTTCGAACGCGTTTGTTGCATTGTTGGCCCATGGAACCTCTCCTTCAATGTCTTCTACGGTGAGAATATGGACGCAAAAAACAAAAAGTCTTTGATCGGAAAAGCATCGTTGGATTTATCGGAGTTAGCGTCGAAACAAGAATCGACGGTGGAGAGAAAGCTTCCGATTCGGTCAAAGGGTTCCGTACTTTCGAAGGAAGCAACACTTGTGGTCAACGTGACTTTTTCTGAGGTAAGAACCGAACCAGACGACTTTATGCAACTTGGTCAGATCTCCGTTGACTCAGCAATTCCGAAGAAAAAGTCAACGAGTCGTCGCGGCGGTGGTAGCCATTTCGACTCTTTTTCCTCCCCAGCCACCGCGTCGAGTTCAGGCGGACGTAGCCCCGTAGTTGAAACCGGGTTATTTTCAAGCCCGGATGAGAACCAGTCTGAACCGGGTCAAAAAGCCGGGTTTAATTGGTGGAAGAGAAGACGGTTAAGCTTTTCTATGACATGGAGAAGAGAACCGAGAGAGGATGAAGTTACGAAGACATCAACAAAACCATCGGAGGAACTAGAAAAACCGGCGACGGAGATACCAATCGAGGCAAACAAATGGGTAATGAAGGATTTAGTGAGCCGCGATGGAAAATCGAAGCTGAAATCGGAAGTTTACTTAGCGTCGATTGATCAAAGAAGCGAACAAGCCGCCGGAGAAGCAGCATGCGCGGCGGTTGCGGTGGTGGTGGCTCATTGGTTCCACGCGAATCCTAAGCTCATCAATCCTTCAGGAACAGCGTTCGATTCACTAATCACGCAAGGGTCTTCTTTATGGCAATCTCTTTGCGACAAAGAATCGTATCTAAGATTGTTTCCAAACAGGCATTTCGATCTGGAAACGATCGTTTCCGCAAATTTACGACCGGTTAGGGTTTGTACCGATAAATCGTTTACCGGGTTATTCAGCCCGGAGAGATTTGCTTCGTTGGATGGTCTGATGTCGTTTGATCAGATTTGGGACGAGTTATCATTTGTCATGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

423

Amino Acids

46.87

Weight (kDa)

8.64

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 34 - 166 2.2e-09 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 7 cut(s) 104, 255, 564, 608, 870, 956, 992
AcsI RAATTY 1 cut(s) 1141
AcuI CTGAAG 1 cut(s) 999
AcvI CACGTG 1 cut(s) 28
AfaI GTAC 2 cut(s) 432, 1165
AfiI CCNNNNNNNGG 1 cut(s) 137
AflIII ACRYGT 1 cut(s) 253
AgeI ACCGGT 2 cut(s) 181, 1150
AgsI TTSAA 3 cut(s) 292, 641, 657
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
AluBI AGCT 6 cut(s) 22, 198, 406, 729, 886, 1003
AluI AGCT 6 cut(s) 22, 198, 406, 729, 886, 1003
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 1 cut(s) 139
AoxI GGCC 1 cut(s) 271
ApeKI GCWGC 1 cut(s) 947
ApoI RAATTY 1 cut(s) 1141
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
AsiGI ACCGGT 2 cut(s) 181, 1150
AspLEI GCGC 1 cut(s) 956
AspS9I GGNCC 2 cut(s) 128, 272
AsuC2I CCSGG 6 cut(s) 646, 662, 684, 697, 1183, 1196
AsuHPI GGTGA 6 cut(s) 25, 46, 55, 86, 127, 317
AsuII TTCGAA 2 cut(s) 249, 437
AvaII GGWCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 200
BbrPI CACGTG 1 cut(s) 28
BbsI GAAGAC 4 cut(s) 289, 724, 782, 1044
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 959
BccI CCATC 5 cut(s) 47, 100, 799, 866, 1211
BceAI ACGGC 1 cut(s) 99
BclI TGATCA 2 cut(s) 916, 1234
BcnI CCSGG 6 cut(s) 646, 662, 684, 697, 1183, 1196
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 1 cut(s) 803
BglI GCCNNNNNGGC 1 cut(s) 46
BglII AGATCT 1 cut(s) 516
BisI GCNGC 5 cut(s) 565, 868, 936, 948, 957
BlsI GCNGC 5 cut(s) 566, 869, 937, 949, 958
Bme1390I CCNGG 6 cut(s) 646, 662, 684, 697, 1183, 1196
Bme18I GGWCC 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 128, 272
BmiI GGNNCC 4 cut(s) 129, 281, 427, 986
BmrFI CCNGG 6 cut(s) 646, 662, 684, 697, 1183, 1196
BmsI GCATC 1 cut(s) 359
BpiI GAAGAC 4 cut(s) 289, 724, 782, 1044
Bpu10I CCTNAGC 1 cut(s) 999
Bpu14I TTCGAA 2 cut(s) 249, 437
BpuMI CCSGG 6 cut(s) 646, 662, 684, 697, 1183, 1196
BsaAI YACGTR 2 cut(s) 28, 229
BsaBI GATNNNNATC 1 cut(s) 827
BsaJI CCNNGG 1 cut(s) 275
BsaWI WCCGGW 3 cut(s) 137, 181, 1150
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 359, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse118I RCCGGY 5 cut(s) 40, 61, 181, 811, 1150
Bse1I ACTGG 1 cut(s) 673
Bse8I GATNNNNATC 1 cut(s) 827
BseDI CCNNGG 1 cut(s) 275
BseGI GGATG 3 cut(s) 670, 769, 1222
BseJI GATNNNNATC 1 cut(s) 827
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMII CTCAG 2 cut(s) 465, 543
BseNI ACTGG 1 cut(s) 673
BseXI GCAGC 1 cut(s) 959
BseYI CCCAGC 1 cut(s) 598
Bsh1236I CGCG 7 cut(s) 104, 255, 564, 608, 870, 956, 992
Bsh1285I CGRYCG 3 cut(s) 182, 1131, 1151
BshFI GGCC 1 cut(s) 273
BshTI ACCGGT 2 cut(s) 181, 1150
BsiEI CGRYCG 3 cut(s) 182, 1131, 1151
BsiHKAI GWGCWC 1 cut(s) 200
BslFI GGGAC 3 cut(s) 141, 143, 1259
BslI CCNNNNNNNGG 1 cut(s) 137
BsmAI GTCTC 1 cut(s) 139
BsmFI GGGAC 3 cut(s) 141, 143, 1259
BsnI GGCC 1 cut(s) 273
Bsp119I TTCGAA 2 cut(s) 249, 437
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 6 cut(s) 339, 516, 916, 1117, 1128, 1234
Bsp19I CCATGG 1 cut(s) 275
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 2 cut(s) 466, 542
BspFNI CGCG 7 cut(s) 104, 255, 564, 608, 870, 956, 992
BspLI GGNNCC 4 cut(s) 129, 281, 427, 986
BspT104I TTCGAA 2 cut(s) 249, 437
BsrFI RCCGGY 5 cut(s) 40, 61, 181, 811, 1150
BsrI ACTGG 1 cut(s) 673
BssAI RCCGGY 5 cut(s) 40, 61, 181, 811, 1150
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 6 cut(s) 339, 516, 916, 1117, 1128, 1234
BssT1I CCWWGG 1 cut(s) 275
Bst4CI ACNGT 4 cut(s) 34, 305, 394, 723
Bst6I CTCTTC 3 cut(s) 237, 707, 742
BstBAI YACGTR 2 cut(s) 28, 229
BstBI TTCGAA 2 cut(s) 249, 437
BstC8I GCNNGC 1 cut(s) 952
BstDEI CTNAG 6 cut(s) 474, 529, 904, 999, 1091, 1269
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 3 cut(s) 670, 769, 1222
BstFNI CGCG 7 cut(s) 104, 255, 564, 608, 870, 956, 992
BstHHI GCGC 1 cut(s) 956
BstKTI GATC 6 cut(s) 342, 519, 919, 1120, 1131, 1237
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 6 cut(s) 339, 516, 916, 1117, 1128, 1234
BstMCI CGRYCG 3 cut(s) 182, 1131, 1151
BstMWI GCNNNNNNNGC 9 cut(s) 46, 261, 270, 573, 627, 944, 953, 956, 962
BstNSI RCATGY 1 cut(s) 954
BstSCI CCNGG 6 cut(s) 644, 660, 682, 695, 1181, 1194
BstUI CGCG 7 cut(s) 104, 255, 564, 608, 870, 956, 992
BstV1I GCAGC 1 cut(s) 959
BstV2I GAAGAC 4 cut(s) 289, 724, 782, 1044
BstX2I RGATCY 1 cut(s) 516
BstYI RGATCY 1 cut(s) 516
BsuRI GGCC 1 cut(s) 273
BtgI CCRYGG 1 cut(s) 275
BtgZI GCGATG 2 cut(s) 119, 885
BtsCI GGATG 3 cut(s) 670, 769, 1222
Cac8I GCNNGC 1 cut(s) 952
CfoI GCGC 1 cut(s) 956
Cfr10I RCCGGY 5 cut(s) 40, 61, 181, 811, 1150
Cfr13I GGNCC 2 cut(s) 128, 272
CseI GACGC 4 cut(s) 326, 363, 597, 897
Csp6I GTAC 2 cut(s) 431, 1164
CspAI ACCGGT 2 cut(s) 181, 1150
CspCI CAANNNNNGTGG 2 cut(s) 435, 470
CviAII CATG 4 cut(s) 276, 741, 951, 1264
CviQI GTAC 2 cut(s) 431, 1164
DdeI CTNAG 6 cut(s) 474, 529, 904, 999, 1091, 1269
DpnI GATC 6 cut(s) 341, 518, 918, 1119, 1130, 1236
DpnII GATC 6 cut(s) 339, 516, 916, 1117, 1128, 1234
Eam1104I CTCTTC 3 cut(s) 237, 707, 742
EarI CTCTTC 3 cut(s) 237, 707, 742
EciI GGCGGA 1 cut(s) 636
Ecl136II GAGCTC 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 128
Eco53kI GAGCTC 1 cut(s) 198
Eco57I CTGAAG 1 cut(s) 999
Eco72I CACGTG 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 198
EcoO109I RGGNCCY 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 275
EcoT38I GRGCYC 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 275
FaeI CATG 4 cut(s) 279, 744, 954, 1267
FaiI YATR 9 cut(s) 192, 277, 314, 503, 737, 742, 952, 1060, 1265
FalI AAGNNNNNCTT 2 cut(s) 435, 467
FaqI GGGAC 3 cut(s) 141, 143, 1259
FatI CATG 4 cut(s) 275, 740, 950, 1263
FbaI TGATCA 2 cut(s) 916, 1234
Fnu4HI GCNGC 5 cut(s) 565, 868, 936, 948, 957
FokI GGATG 3 cut(s) 677, 776, 1229
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 5 cut(s) 565, 868, 936, 948, 957
FspBI CTAG 1 cut(s) 803
GlaI GCGC 1 cut(s) 955
GluI GCNGC 5 cut(s) 565, 868, 936, 948, 957
GsaI CCCAGC 1 cut(s) 602
HaeIII GGCC 1 cut(s) 273
HgaI GACGC 4 cut(s) 326, 363, 597, 897
HhaI GCGC 1 cut(s) 956
Hin1II CATG 4 cut(s) 279, 744, 954, 1267
Hin6I GCGC 1 cut(s) 954
HinP1I GCGC 1 cut(s) 954
HincII GTYRAC 4 cut(s) 187, 460, 526, 552
HindII GTYRAC 4 cut(s) 187, 460, 526, 552
HindIII AAGCTT 3 cut(s) 20, 404, 727
HinfI GANTC 8 cut(s) 386, 412, 527, 556, 584, 994, 1031, 1082
HpaI GTTAAC 1 cut(s) 187
HphI GGTGA 6 cut(s) 25, 46, 55, 86, 127, 317
Hpy166II GTNNAC 7 cut(s) 59, 187, 460, 526, 552, 901, 1179
Hpy188III TCNNGA 2 cut(s) 1017, 1121
Hpy8I GTNNAC 7 cut(s) 59, 187, 460, 526, 552, 901, 1179
Hpy99I CGWCG 6 cut(s) 379, 394, 564, 613, 820, 913
HpyAV CCTTC 6 cut(s) 4, 118, 298, 433, 847, 1023
HpyCH4III ACNGT 4 cut(s) 34, 305, 394, 723
HpyCH4IV ACGT 4 cut(s) 27, 228, 462, 625
HpyCH4V TGCA 2 cut(s) 264, 505
HpyF10VI GCNNNNNNNGC 9 cut(s) 46, 261, 270, 573, 627, 944, 953, 956, 962
HpyF3I CTNAG 6 cut(s) 474, 529, 904, 999, 1091, 1269
HpySE526I ACGT 4 cut(s) 27, 228, 462, 625
Hsp92II CATG 4 cut(s) 279, 744, 954, 1267
HspAI GCGC 1 cut(s) 954
Ksp22I TGATCA 2 cut(s) 916, 1234
KspAI GTTAAC 1 cut(s) 187
Kzo9I GATC 6 cut(s) 339, 516, 916, 1117, 1128, 1234
Lsp1109I GCAGC 1 cut(s) 959
LweI GCATC 1 cut(s) 359
MaeI CTAG 1 cut(s) 803
MaeII ACGT 4 cut(s) 27, 228, 462, 625
MaeIII GTNAC 3 cut(s) 28, 463, 769
MalI GATC 6 cut(s) 341, 518, 918, 1119, 1130, 1236
MboI GATC 6 cut(s) 339, 516, 916, 1117, 1128, 1234
MboII GAAGA 8 cut(s) 254, 289, 553, 724, 729, 759, 787, 1044
MflI RGATCY 1 cut(s) 516
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 3 cut(s) 534, 703, 1141
MluI ACGCGT 1 cut(s) 253
MlyI GAGTC 3 cut(s) 521, 565, 578
MmeI TCCRAC 2 cut(s) 336, 1194
MseI TTAA 3 cut(s) 186, 702, 725
MspR9I CCNGG 6 cut(s) 646, 662, 684, 697, 1183, 1196
MvnI CGCG 7 cut(s) 104, 255, 564, 608, 870, 956, 992
MwoI GCNNNNNNNGC 9 cut(s) 46, 261, 270, 573, 627, 944, 953, 956, 962
NciI CCSGG 6 cut(s) 646, 662, 684, 697, 1183, 1196
NcoI CCATGG 1 cut(s) 275
NdeII GATC 6 cut(s) 339, 516, 916, 1117, 1128, 1234
NlaIII CATG 4 cut(s) 279, 744, 954, 1267
NlaIV GGNNCC 4 cut(s) 129, 281, 427, 986
NmuCI GTSAC 2 cut(s) 28, 463
NspI RCATGY 1 cut(s) 954
NspV TTCGAA 2 cut(s) 249, 437
PaeI GCATGC 1 cut(s) 954
PcsI WCGNNNNNNNCGW 2 cut(s) 175, 371
PfeI GAWTC 5 cut(s) 386, 412, 994, 1031, 1082
PinAI ACCGGT 2 cut(s) 181, 1150
PkrI GCNGC 5 cut(s) 566, 869, 937, 949, 958
Ple19I CGATCG 1 cut(s) 1131
PleI GAGTC 3 cut(s) 521, 564, 578
PmaCI CACGTG 1 cut(s) 28
PmlI CACGTG 1 cut(s) 28
PpsI GAGTC 3 cut(s) 521, 564, 578
Ppu21I YACGTR 2 cut(s) 28, 229
PpuMI RGGWCCY 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 200
Psp5II RGGWCCY 1 cut(s) 128
PspCI CACGTG 1 cut(s) 28
PspFI CCCAGC 1 cut(s) 598
PspN4I GGNNCC 4 cut(s) 129, 281, 427, 986
PspPI GGNCC 2 cut(s) 128, 272
PspPPI RGGWCCY 1 cut(s) 128
PsuI RGATCY 1 cut(s) 516
PvuI CGATCG 1 cut(s) 1131
RsaI GTAC 2 cut(s) 432, 1165
RsaNI GTAC 2 cut(s) 431, 1164
SacI GAGCTC 1 cut(s) 200
SaqAI TTAA 3 cut(s) 186, 702, 725
SatI GCNGC 5 cut(s) 565, 868, 936, 948, 957
Sau3AI GATC 6 cut(s) 339, 516, 916, 1117, 1128, 1234
Sau96I GGNCC 2 cut(s) 128, 272
SchI GAGTC 3 cut(s) 521, 565, 578
ScrFI CCNGG 6 cut(s) 646, 662, 684, 697, 1183, 1196
SduI GDGCHC 1 cut(s) 200
SfaNI GCATC 1 cut(s) 359
SfuI TTCGAA 2 cut(s) 249, 437
SinI GGWCC 1 cut(s) 128
SphI GCATGC 1 cut(s) 954
Sse9I AATT 3 cut(s) 534, 703, 1141
SspMI CTAG 1 cut(s) 803
SstI GAGCTC 1 cut(s) 200
StyD4I CCNGG 6 cut(s) 644, 660, 682, 695, 1181, 1194
StyI CCWWGG 1 cut(s) 275
TaaI ACNGT 4 cut(s) 34, 305, 394, 723
TaiI ACGT 4 cut(s) 30, 231, 465, 628
TaqII GACCGA 1 cut(s) 405
TasI AATT 3 cut(s) 534, 703, 1141
TauI GCSGC 4 cut(s) 567, 870, 938, 959
TfiI GAWTC 5 cut(s) 386, 412, 994, 1031, 1082
Tru1I TTAA 3 cut(s) 186, 702, 725
Tru9I TTAA 3 cut(s) 186, 702, 725
TseFI GTSAC 2 cut(s) 28, 463
TseI GCWGC 1 cut(s) 947
Tsp45I GTSAC 2 cut(s) 28, 463
TspDTI ATGAA 3 cut(s) 93, 780, 866
TspGWI ACGGA 3 cut(s) 418, 511, 833
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 1 cut(s) 1141
XceI RCATGY 1 cut(s) 954
XspI CTAG 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.