MD08G1150600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009226
Physical Location & Seq
Forward (+)
15317364 .. 15320468
3105 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1150600.v1.1.491

Sequence Viewer

Length: 1905 bp
ATGAAGTGGTCGCCGTGGCCGGGTGGGGGAACAAAGAGGTTTCACGTGAAGGTGAGCCAATTGAAGCTGCAAGGGTTTAATTTTGACAAGGAGAAAGATAGAGTTGGTGTGATTGAGGTGAAATGGAAAGGGTATAAGCCTGGAGGGCCTGGAGTATTGGTTCCATTCTACAGAAGAAGTTCGTCGAGGCATCCGAAAAACTACACCAGCCATAGATTTTTGAGTCGTCAAGTCATTGAATGGGATGACGAATTTCAGAAGCTCTGTAATTTTGGGTTAAAGCAAGGTGGTAACTTTAGTCCTTGGGATTTGACTTTTACCCTGTTACACGTATATAGGAAGAGCACATCAGCTCAAGCCTATGTGGGAGCGAGTACAGAACAGAAGAGTAATATGGTGGTTTTCGGGACGGTTTCGTTGAATCTAGCAGAATTGCCATCGACAATGGATTCTCAGATTCAGAGGAAGCTCTCTGTTACTTTGAAAATGGAGGGGACGACTCCCAGCGAAGCTACCCTTTTGGTTTGTCTAAGCTTTGCTGAGGTCAGAAACTCGCATGGCTCGGCAGGACTCGGTCAAGACTCGGCCGAGTCAGACAAGGGGAAATTGACTCGAAGGGTAAGCTACTTCAGGAGCTTTAGAAAGTCAAGCAATGAGAGGAAGAGTCGTGGGGACCAAGTCACGAGTGACTGGCACGAGTCATTCTTGTGTGACTCAGAAGGGTCCCCGGGGAACGGACTCATATCCAGTGATGTCAATGCAGACTTGAATTCAAAGGGTGAGTCGGGATCTTTCCCGAGTCACGAGACTCAGTTTGACTCGGGGGAGAAGAGCTGGTTCTTGAGGAAGAGGAAGTGGTTGAATGGAAGACCGCCTAGGAGAAAAGTGGAGCTCCTTGTTGGGAAGACTAGTAGAGCTGTTGACAATAATCTCCCTGATTCTGTAAAGGAATCAAATAGTACTGCTGAATATTCTTCACAATCAGATCATGAACTTGGAAATTACACCACAAGTAGATGGGAAGAAAGGGAAGTAGAAAGCAGAGATGGGCAAGCAAAGCTCACGACCAATGTGTTCTTTGCCTCCTTTGATCAACGGAGTGAAAAGGCCGCCGGAGAGAGTGCCTGCACAGCGCTAGTTGCGGTCATTGCGCACTGGCTGCATTTGCATCAACACTTGATGCCTACAAGATCCAAATTCGACAGCCTCATCACACAAGGTTCCTCAGAGTGGCAAAAACTCTGCAGCAACGAGACCTACATAAATTTATTTCCCAACAAACACTTCGACCTTGAAACAGTTTTGGAAGCTGATCTTAGACCTCTCGATGTCTTGCCTGAGAAATCCTTTATTGGATTCTTCAGCCCCGAGAAGTTCGAGAGCTTGAAGGGACTCATGTCGTTTGATGACATATGGAATGAAATAAATAAAAACACAAAGTCTTACGAACCGAGGATATACATAGTAAGTTGGAACGACCACTTTTTCGTTCTGAAGATTGAAGCCGATGCATACTATATGATTGACTCTTTGGGCGAGAGGCTCTTTGAGGGGTGTAACCAGGCATACATACTAAAATTCGACAATTCGAGCATCATGCATGGGAAAACAAAGAAAGTAGAGGATGATTCAGAGGAAAGATTAGAAGTAATATGCAGTGGAAAAGAGTGCTGTAGACAGTTCATTAAAAGATTTCTTGCGGCAATACCACTTAAGGAGCTTGAGGAGGAAGAGAAGAAGAGTGCATCTTCTATTTTACCTCTTCACCGGCATTTGCAGATCGAGTTCCACTACACCTCCTCCTCCTCTTCGTCATCATCGTCATCGTCATCATCGTTGTCGTCCTTCTCCTCCTCCACTTTGTCGACATCTTCGACTTCTTCCACTTACTCTCTTCTCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

635

Amino Acids

71.72

Weight (kDa)

7.95

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 37 - 179 5.2e-08 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1152
AccI GTMKAC 2 cut(s) 1675, 1865
AciI CCGC 4 cut(s) 872, 1110, 1142, 1700
AclWI GGATC 2 cut(s) 796, 1185
AcoI YGGCCR 2 cut(s) 17, 585
AcsI RAATTY 5 cut(s) 251, 769, 1196, 1264, 1577
AcuI CTGAAG 3 cut(s) 613, 1345, 1514
AcvI CACGTG 1 cut(s) 46
AfaI GTAC 2 cut(s) 376, 961
AfeI AGCGCT 1 cut(s) 1134
AfiI CCNNNNNNNGG 4 cut(s) 20, 26, 734, 1230
AflII CTTAAG 1 cut(s) 1712
AflIII ACRYGT 1 cut(s) 328
AhlI ACTAGT 1 cut(s) 908
AjnI CCWGG 3 cut(s) 139, 148, 1560
AjuI GAANNNNNNNTTGG 2 cut(s) 1268, 1300
AloI GAACNNNNNNTCC 2 cut(s) 144, 176
Alw21I GWGCWC 2 cut(s) 347, 894
Alw26I GTCTC 2 cut(s) 800, 1247
AlwI GGATC 2 cut(s) 796, 1185
Ama87I CYCGRG 4 cut(s) 727, 796, 820, 1367
Aor51HI AGCGCT 1 cut(s) 1134
AoxI GGCC 4 cut(s) 17, 146, 585, 1107
ApeKI GCWGC 3 cut(s) 67, 1159, 1245
ApoI RAATTY 5 cut(s) 251, 769, 1196, 1264, 1577
ArsI GACNNNNNNTTYG 4 cut(s) 767, 799, 1469, 1501
Asp700I GAANNNNTTC 1 cut(s) 178
AspA2I CCTAGG 1 cut(s) 875
AspLEI GCGC 2 cut(s) 1135, 1153
AspS9I GGNCC 3 cut(s) 146, 673, 723
AsuC2I CCSGG 3 cut(s) 21, 728, 729
AsuHPI GGTGA 4 cut(s) 64, 130, 791, 1757
AvaI CYCGRG 4 cut(s) 727, 796, 820, 1367
AvaII GGWCC 2 cut(s) 673, 723
AvrII CCTAGG 1 cut(s) 875
BanII GRGCYC 1 cut(s) 894
BauI CACGAG 3 cut(s) 682, 695, 803
BbrPI CACGTG 1 cut(s) 46
BbsI GAAGAC 2 cut(s) 874, 911
Bbv12I GWGCWC 2 cut(s) 347, 894
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 3 cut(s) 54, 1146, 1257
BccI CCATC 3 cut(s) 445, 1011, 1040
BcgI CGANNNNNNTGC 2 cut(s) 1579, 1613
BciT130I CCWGG 3 cut(s) 141, 150, 1562
BclI TGATCA 1 cut(s) 1090
BcnI CCSGG 3 cut(s) 21, 728, 729
BcoDI GTCTC 2 cut(s) 800, 1247
BcuI ACTAGT 1 cut(s) 908
BfaI CTAG 4 cut(s) 425, 876, 909, 1136
BfmI CTRYAG 3 cut(s) 169, 1243, 1672
BfoI RGCGCY 1 cut(s) 1136
BfrI CTTAAG 1 cut(s) 1712
BglI GCCNNNNNGGC 1 cut(s) 145
BisI GCNGC 5 cut(s) 68, 1110, 1160, 1246, 1701
BlnI CCTAGG 1 cut(s) 875
BlsI GCNGC 5 cut(s) 69, 1111, 1161, 1247, 1702
BmcAI AGTACT 1 cut(s) 961
Bme1390I CCNGG 6 cut(s) 21, 141, 150, 728, 729, 1562
Bme18I GGWCC 2 cut(s) 673, 723
BmeT110I CYCGRG 4 cut(s) 727, 796, 820, 1367
BmgT120I GGNCC 3 cut(s) 146, 673, 723
BmiI GGNNCC 5 cut(s) 162, 674, 724, 725, 1222
BmrFI CCNGG 6 cut(s) 21, 141, 150, 728, 729, 1562
BmsI GCATC 6 cut(s) 199, 1170, 1177, 1498, 1602, 1754
BoxI GACNNNNGTC 1 cut(s) 1396
BpiI GAAGAC 2 cut(s) 874, 911
BpmI CTGGAG 2 cut(s) 162, 171
Bpu10I CCTNAGC 1 cut(s) 540
BpuEI CTTGAG 3 cut(s) 339, 862, 1742
BpuMI CCSGG 3 cut(s) 21, 728, 729
BsaAI YACGTR 2 cut(s) 46, 331
BsaI GGTCTC 1 cut(s) 1247
BsaJI CCNNGG 7 cut(s) 14, 302, 726, 727, 728, 875, 1451
BsaXI ACNNNNNCTCC 6 cut(s) 144, 174, 1781, 1784, 1811, 1814
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 26, 734, 1230
Bse118I RCCGGY 1 cut(s) 1767
Bse1I ACTGG 3 cut(s) 695, 747, 1160
Bse3DI GCAATG 2 cut(s) 658, 1146
BseBI CCWGG 3 cut(s) 141, 150, 1562
BseDI CCNNGG 7 cut(s) 14, 302, 726, 727, 728, 875, 1451
BseGI GGATG 3 cut(s) 190, 250, 1630
BseLI CCNNNNNNNGG 4 cut(s) 20, 26, 734, 1230
BseMI GCAATG 2 cut(s) 658, 1146
BseMII CTCAG 6 cut(s) 467, 531, 729, 824, 1239, 1329
BseNI ACTGG 3 cut(s) 695, 747, 1160
BseRI GAGGAG 6 cut(s) 1739, 1789, 1792, 1795, 1840, 1843
BseX3I CGGCCG 1 cut(s) 585
BseXI GCAGC 3 cut(s) 54, 1146, 1257
BseYI CCCAGC 1 cut(s) 503
BsgI GTGCAG 1 cut(s) 1111
Bsh1285I CGRYCG 1 cut(s) 588
BshFI GGCC 4 cut(s) 19, 148, 587, 1109
BsiEI CGRYCG 1 cut(s) 588
BsiHKAI GWGCWC 2 cut(s) 347, 894
BsiHKCI CYCGRG 4 cut(s) 727, 796, 820, 1367
BsiSI CCGG 4 cut(s) 20, 728, 1113, 1768
BslFI GGGAC 5 cut(s) 421, 508, 686, 709, 1404
BslI CCNNNNNNNGG 4 cut(s) 20, 26, 734, 1230
BsmAI GTCTC 2 cut(s) 800, 1247
BsmFI GGGAC 5 cut(s) 421, 508, 686, 709, 1404
BsnI GGCC 4 cut(s) 19, 148, 587, 1109
Bso31I GGTCTC 1 cut(s) 1247
BsoBI CYCGRG 4 cut(s) 727, 796, 820, 1367
Bsp1286I GDGCHC 2 cut(s) 347, 894
Bsp143I GATC 6 cut(s) 788, 985, 1090, 1190, 1312, 1779
BspACI CCGC 4 cut(s) 872, 1110, 1142, 1700
BspANI GGCC 4 cut(s) 19, 148, 587, 1109
BspCNI CTCAG 6 cut(s) 466, 532, 728, 823, 1238, 1330
BspHI TCATGA 1 cut(s) 988
BspLI GGNNCC 5 cut(s) 162, 674, 724, 725, 1222
BspMAI CTGCAG 1 cut(s) 1247
BspPI GGATC 2 cut(s) 796, 1185
BspQI GCTCTTC 2 cut(s) 335, 824
BspTI CTTAAG 1 cut(s) 1712
BspTNI GGTCTC 1 cut(s) 1247
BsrDI GCAATG 2 cut(s) 658, 1146
BsrFI RCCGGY 1 cut(s) 1767
BsrI ACTGG 3 cut(s) 695, 747, 1160
BssAI RCCGGY 1 cut(s) 1767
BssECI CCNNGG 7 cut(s) 14, 302, 726, 727, 728, 875, 1451
BssMI GATC 6 cut(s) 788, 985, 1090, 1190, 1312, 1779
BssSI CACGAG 3 cut(s) 682, 695, 803
BssT1I CCWWGG 2 cut(s) 302, 875
Bst2BI CACGAG 3 cut(s) 682, 695, 803
Bst2UI CCWGG 3 cut(s) 141, 150, 1562
Bst4CI ACNGT 3 cut(s) 412, 1300, 1680
BstAFI CTTAAG 1 cut(s) 1712
BstAPI GCANNNNNTGC 1 cut(s) 1159
BstBAI YACGTR 2 cut(s) 46, 331
BstC8I GCNNGC 2 cut(s) 1053, 1126
BstDEI CTNAG 9 cut(s) 453, 530, 540, 715, 810, 1225, 1316, 1338, 1898
BstDSI CCRYGG 1 cut(s) 14
BstF5I GGATG 3 cut(s) 190, 250, 1630
BstH2I RGCGCY 1 cut(s) 1136
BstHHI GCGC 2 cut(s) 1135, 1153
BstKTI GATC 6 cut(s) 791, 988, 1093, 1193, 1315, 1782
BstMAI GTCTC 2 cut(s) 800, 1247
BstMBI GATC 6 cut(s) 788, 985, 1090, 1190, 1312, 1779
BstMCI CGRYCG 1 cut(s) 588
BstMWI GCNNNNNNNGC 7 cut(s) 145, 1057, 1130, 1139, 1148, 1159, 1165
BstNI CCWGG 3 cut(s) 141, 150, 1562
BstPAI GACNNNNGTC 1 cut(s) 1396
BstSCI CCNGG 6 cut(s) 19, 139, 148, 726, 727, 1560
BstSFI CTRYAG 3 cut(s) 169, 1243, 1672
BstV1I GCAGC 3 cut(s) 54, 1146, 1257
BstV2I GAAGAC 2 cut(s) 874, 911
BstX2I RGATCY 2 cut(s) 788, 1190
BstYI RGATCY 2 cut(s) 788, 1190
BstZI CGGCCG 1 cut(s) 585
BsuRI GGCC 4 cut(s) 19, 148, 587, 1109
BtgI CCRYGG 1 cut(s) 14
BtsCI GGATG 3 cut(s) 190, 250, 1630
BtsI GCAGTG 1 cut(s) 1663
BtsIMutI CAGTG 3 cut(s) 754, 1153, 1663
Cac8I GCNNGC 2 cut(s) 1053, 1126
CciI TCATGA 1 cut(s) 988
CfoI GCGC 2 cut(s) 1135, 1153
Cfr10I RCCGGY 1 cut(s) 1767
Cfr13I GGNCC 3 cut(s) 146, 673, 723
Cfr9I CCCGGG 1 cut(s) 727
Csp6I GTAC 2 cut(s) 375, 960
CviAII CATG 5 cut(s) 557, 989, 1396, 1597, 1601
CviQI GTAC 2 cut(s) 375, 960
DdeI CTNAG 9 cut(s) 453, 530, 540, 715, 810, 1225, 1316, 1338, 1898
DpnI GATC 6 cut(s) 790, 987, 1092, 1192, 1314, 1781
DpnII GATC 6 cut(s) 788, 985, 1090, 1190, 1312, 1779
EaeI YGGCCR 2 cut(s) 17, 585
EagI CGGCCG 1 cut(s) 585
Ecl136II GAGCTC 1 cut(s) 892
EclXI CGGCCG 1 cut(s) 585
Eco130I CCWWGG 2 cut(s) 302, 875
Eco24I GRGCYC 1 cut(s) 894
Eco31I GGTCTC 1 cut(s) 1247
Eco47I GGWCC 2 cut(s) 673, 723
Eco47III AGCGCT 1 cut(s) 1134
Eco52I CGGCCG 1 cut(s) 585
Eco53kI GAGCTC 1 cut(s) 892
Eco57I CTGAAG 3 cut(s) 613, 1345, 1514
Eco72I CACGTG 1 cut(s) 46
Eco88I CYCGRG 4 cut(s) 727, 796, 820, 1367
EcoICRI GAGCTC 1 cut(s) 892
EcoO109I RGGNCCY 2 cut(s) 146, 723
EcoRI GAATTC 1 cut(s) 769
EcoRII CCWGG 3 cut(s) 139, 148, 1560
EcoT14I CCWWGG 2 cut(s) 302, 875
EcoT22I ATGCAT 2 cut(s) 1513, 1602
EcoT38I GRGCYC 1 cut(s) 894
ErhI CCWWGG 2 cut(s) 302, 875
FaeI CATG 5 cut(s) 560, 992, 1399, 1600, 1604
FalI AAGNNNNNCTT 4 cut(s) 501, 533, 1299, 1331
FaqI GGGAC 5 cut(s) 421, 508, 686, 709, 1404
FatI CATG 5 cut(s) 556, 988, 1395, 1596, 1600
FauNDI CATATG 1 cut(s) 1412
FbaI TGATCA 1 cut(s) 1090
FblI GTMKAC 2 cut(s) 1675, 1865
Fnu4HI GCNGC 5 cut(s) 68, 1110, 1160, 1246, 1701
FokI GGATG 3 cut(s) 177, 257, 1637
FriOI GRGCYC 1 cut(s) 894
Fsp4HI GCNGC 5 cut(s) 68, 1110, 1160, 1246, 1701
FspBI CTAG 4 cut(s) 425, 876, 909, 1136
FspI TGCGCA 1 cut(s) 1152
GlaI GCGC 2 cut(s) 1134, 1152
GluI GCNGC 5 cut(s) 68, 1110, 1160, 1246, 1701
GsaI CCCAGC 1 cut(s) 507
GsuI CTGGAG 2 cut(s) 162, 171
HaeII RGCGCY 1 cut(s) 1136
HaeIII GGCC 4 cut(s) 19, 148, 587, 1109
HapII CCGG 4 cut(s) 20, 728, 1113, 1768
HhaI GCGC 2 cut(s) 1135, 1153
Hin1II CATG 5 cut(s) 560, 992, 1399, 1600, 1604
Hin6I GCGC 2 cut(s) 1133, 1151
HinP1I GCGC 2 cut(s) 1133, 1151
HincII GTYRAC 2 cut(s) 922, 1866
HindII GTYRAC 2 cut(s) 922, 1866
HindIII AAGCTT 1 cut(s) 532
HpaII CCGG 4 cut(s) 20, 728, 1113, 1768
HphI GGTGA 4 cut(s) 64, 130, 791, 1757
Hpy166II GTNNAC 3 cut(s) 922, 1676, 1866
Hpy8I GTNNAC 3 cut(s) 922, 1676, 1866
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 5 cut(s) 43, 609, 713, 1381, 1855
HpyCH4III ACNGT 3 cut(s) 412, 1300, 1680
HpyCH4IV ACGT 2 cut(s) 45, 330
HpyF10VI GCNNNNNNNGC 7 cut(s) 145, 1057, 1130, 1139, 1148, 1159, 1165
HpyF3I CTNAG 9 cut(s) 453, 530, 540, 715, 810, 1225, 1316, 1338, 1898
HpySE526I ACGT 2 cut(s) 45, 330
Hsp92II CATG 5 cut(s) 560, 992, 1399, 1600, 1604
HspAI GCGC 2 cut(s) 1133, 1151
KflI GGGWCCC 1 cut(s) 723
Ksp22I TGATCA 1 cut(s) 1090
Kzo9I GATC 6 cut(s) 788, 985, 1090, 1190, 1312, 1779
LguI GCTCTTC 2 cut(s) 335, 824
LmnI GCTCC 5 cut(s) 368, 633, 889, 897, 1717
Lsp1109I GCAGC 3 cut(s) 54, 1146, 1257
LweI GCATC 6 cut(s) 199, 1170, 1177, 1498, 1602, 1754
MaeI CTAG 4 cut(s) 425, 876, 909, 1136
MaeII ACGT 2 cut(s) 45, 330
MaeIII GTNAC 8 cut(s) 290, 324, 475, 679, 686, 710, 800, 1556
MalI GATC 6 cut(s) 790, 987, 1092, 1192, 1314, 1781
MboI GATC 6 cut(s) 788, 985, 1090, 1190, 1312, 1779
MfeI CAATTG 1 cut(s) 59
MflI RGATCY 2 cut(s) 788, 1190
MhlI GDGCHC 2 cut(s) 347, 894
MmeI TCCRAC 1 cut(s) 1451
Mph1103I ATGCAT 2 cut(s) 1513, 1602
MroXI GAANNNNTTC 1 cut(s) 178
MseI TTAA 4 cut(s) 78, 278, 1686, 1713
MslI CAYNNNNRTG 1 cut(s) 706
MspCI CTTAAG 1 cut(s) 1712
MspI CCGG 4 cut(s) 20, 728, 1113, 1768
MspR9I CCNGG 6 cut(s) 21, 141, 150, 728, 729, 1562
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 3 cut(s) 141, 150, 1562
MwoI GCNNNNNNNGC 7 cut(s) 145, 1057, 1130, 1139, 1148, 1159, 1165
NciI CCSGG 3 cut(s) 21, 728, 729
NdeI CATATG 1 cut(s) 1412
NdeII GATC 6 cut(s) 788, 985, 1090, 1190, 1312, 1779
NlaIII CATG 5 cut(s) 560, 992, 1399, 1600, 1604
NlaIV GGNNCC 5 cut(s) 162, 674, 724, 725, 1222
NmeAIII GCCGAG 3 cut(s) 542, 563, 613
NmuCI GTSAC 4 cut(s) 679, 686, 710, 800
NsbI TGCGCA 1 cut(s) 1152
NsiI ATGCAT 2 cut(s) 1513, 1602
PagI TCATGA 1 cut(s) 988
PciSI GCTCTTC 2 cut(s) 335, 824
PcsI WCGNNNNNNNCGW 4 cut(s) 191, 1817, 1832, 1871
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 7 cut(s) 421, 449, 457, 938, 950, 1356, 1628
PflFI GACNNNGTC 2 cut(s) 573, 677
PkrI GCNGC 5 cut(s) 69, 1111, 1161, 1247, 1702
PmaCI CACGTG 1 cut(s) 46
PmlI CACGTG 1 cut(s) 46
Ppu21I YACGTR 2 cut(s) 46, 331
PpuMI RGGWCCY 1 cut(s) 723
PshAI GACNNNNGTC 1 cut(s) 1396
Psp124BI GAGCTC 1 cut(s) 894
Psp5II RGGWCCY 1 cut(s) 723
Psp6I CCWGG 3 cut(s) 139, 148, 1560
PspCI CACGTG 1 cut(s) 46
PspFI CCCAGC 1 cut(s) 503
PspGI CCWGG 3 cut(s) 139, 148, 1560
PspN4I GGNNCC 5 cut(s) 162, 674, 724, 725, 1222
PspPI GGNCC 3 cut(s) 146, 673, 723
PspPPI RGGWCCY 1 cut(s) 723
PstI CTGCAG 1 cut(s) 1247
PsuI RGATCY 2 cut(s) 788, 1190
PsyI GACNNNGTC 2 cut(s) 573, 677
RsaI GTAC 2 cut(s) 376, 961
RsaNI GTAC 2 cut(s) 375, 960
RseI CAYNNNNRTG 1 cut(s) 706
SacI GAGCTC 1 cut(s) 894
SalI GTCGAC 1 cut(s) 1864
SapI GCTCTTC 2 cut(s) 335, 824
SaqAI TTAA 4 cut(s) 78, 278, 1686, 1713
SatI GCNGC 5 cut(s) 68, 1110, 1160, 1246, 1701
Sau3AI GATC 6 cut(s) 788, 985, 1090, 1190, 1312, 1779
Sau96I GGNCC 3 cut(s) 146, 673, 723
ScaI AGTACT 1 cut(s) 961
ScrFI CCNGG 6 cut(s) 21, 141, 150, 728, 729, 1562
SduI GDGCHC 2 cut(s) 347, 894
SfaNI GCATC 6 cut(s) 199, 1170, 1177, 1498, 1602, 1754
SfcI CTRYAG 3 cut(s) 169, 1243, 1672
SinI GGWCC 2 cut(s) 673, 723
SmaI CCCGGG 1 cut(s) 729
SmiMI CAYNNNNRTG 1 cut(s) 706
SmlI CTYRAG 4 cut(s) 354, 841, 1712, 1721
SmoI CTYRAG 4 cut(s) 354, 841, 1712, 1721
SpeI ACTAGT 1 cut(s) 908
SsiI CCGC 4 cut(s) 872, 1110, 1142, 1700
SspI AATATT 1 cut(s) 971
SspMI CTAG 4 cut(s) 425, 876, 909, 1136
SstI GAGCTC 1 cut(s) 894
StyD4I CCNGG 6 cut(s) 19, 139, 148, 726, 727, 1560
StyI CCWWGG 2 cut(s) 302, 875
TaaI ACNGT 3 cut(s) 412, 1300, 1680
TaiI ACGT 2 cut(s) 48, 333
TaqII GACCGA 1 cut(s) 563
TatI WGTACW 2 cut(s) 374, 959
TauI GCSGC 2 cut(s) 1112, 1703
TfiI GAWTC 7 cut(s) 421, 449, 457, 938, 950, 1356, 1628
Tru1I TTAA 4 cut(s) 78, 278, 1686, 1713
Tru9I TTAA 4 cut(s) 78, 278, 1686, 1713
TscAI CASTG 3 cut(s) 754, 1160, 1663
TseFI GTSAC 4 cut(s) 679, 686, 710, 800
TseI GCWGC 3 cut(s) 67, 1159, 1245
Tsp45I GTSAC 4 cut(s) 679, 686, 710, 800
TspDTI ATGAA 4 cut(s) 17, 1005, 1434, 1672
TspGWI ACGGA 2 cut(s) 750, 1111
TspMI CCCGGG 1 cut(s) 727
TspRI CASTG 3 cut(s) 754, 1160, 1663
Tth111I GACNNNGTC 2 cut(s) 573, 677
Vha464I CTTAAG 1 cut(s) 1712
VpaK11BI GGWCC 2 cut(s) 673, 723
XapI RAATTY 5 cut(s) 251, 769, 1196, 1264, 1577
XmaI CCCGGG 1 cut(s) 727
XmaJI CCTAGG 1 cut(s) 875
XmiI GTMKAC 2 cut(s) 1675, 1865
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 4 cut(s) 425, 876, 909, 1136
ZrmI AGTACT 1 cut(s) 961
Zsp2I ATGCAT 2 cut(s) 1513, 1602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.