MD00G1144300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009226
Physical Location & Seq
Forward (+)
31640722 .. 31643897
3176 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1144300.v1.1.491

Sequence Viewer

Length: 1863 bp
ATGAAGTGGTCGCCGTGGCCGTCAGGCGGAACAAAGAGGTTTCACGTGAAGGTGAGCCAGCTGAAGCTGCAAGGGTTTAATTTTGAGAAGGAGAAAGAGAGTGTTGGTGTGATTGAGGTGAAATGGAAAGGGTCTAAGCCTGGAGGGCCTGGAGTTTTGGTTCCATTCTATGGAAGAAGATCTTCCAGGCAGCCGAAAAACTACACCAGCCAGAGATTTTTGACTCGTCAAGTCATTGAATGGGATGATGAATTCCAGAGCCTCTGTAATTTTGGGTCGAAGCAAGGTGGGAACTTTAGTCCCTGGGATTTGACTTTTACCCTGTTACACGGAGAGAGTGCGGAGCAAAAGAGTAATATGGCGGTTTTCGGGACCGTTTCGTTGAACCTAGCAGAATTGGCATCGACAATGGAGTCTCAGATTCCGAGGAAGCTCTCTGTTGCTTTGAAAATGGAGGGGACGACTCCCAGCGAAGCTACCCTTTTGGTTTGTCTAATAAGCTTTGCTGAGGTGAGAAACTTGTATGACTCGGAAGCACTCGGTCAAGACTCGACCAAGTCAGACAAGGGCAAGTTGGTTCGAAGAGTAAGCTACTTCAGGAGCTTTAGGAAGTCAAGCGATGAGAGGAAGAGTCGTGAGGACCGGGGCATGAGTGACCGGGACGAGTCATTCTTGTCTGACTCAGAAGGGTCACTGGGGAATGGACTCGTTAACGGTGGTGTCAATGCAGATTTGAGTTCAAAGGGAGAGTCGGGGTCGTTCCCGAGTCATGAGACTCAGTCTGACTCGGGGCAGAAGAGTTGGCTCTCGAGGAAGAGGAAGTGGTTGAATGGAAGACCACCTAGGAGAAAAGTGGAGCCCTTTGTTGAGAAGACTAGTAGGGACAATGCAGTGCCAATTTCCGTTGACAACAATCTCAGTGATTCTATAAAGATATCAAATTGTACTGCTGAATATTCTTCACAATCAGATCATGAGCATGGAAAATGCACCACAAATTGCTGGGAAGAAAGGGAAGTAGAAAGCAGAGATGGGCAAGCAAAGCTCAAAACCAATGCGTTCTTTGCGTCCTTTGATCAACGGAGTGAAAAGGCCGCCGGAGAGAGTGCCTGCACAGCCCTTGTTGCGGTCATTGCGCACTGGCTGCATTCGCACCAACACTCAATGCCTACAAGATCCAAATTCGACAGCCTCATCACACAAGGTTCCTCAGAGTGGCAAAAACTCTGCAGCAACGAGACCTACATAAACCTATTTCCCAACAAACACTTCGACCTTGAAACGGTTTTGGAAGCTGATCTTAGACCTGTTGGTGTCTCGCCTGAGAAATCCTTTATCGGATTCTTCAGCCCGGAGAAGTTCGAGAGCTTGAAGGGACTCATGTCGTTCGACGACATATGGAATGAGATAGATAAAAACAGAGAGTTTTACGAACCGAGGATATACATAGTAAGTTGGAACGACCATTTTTTCGTGCTGAAGGTTGAAGCTGATGCATACTATATCATTGACTCTTTGGGTGAGAGGCTCTTTGAGGGGTGTAACCAGGCATACATACTAAAATTCGACGATTCGAGCATCATGCATGGGAAAACAGAGAAAGCAGAGGATGATTCAGAGGAAAGATTAGAAGTAATATGCAGTGGAAAAGAGTGTTGTAGACAGTTCATTAAAAGATTTCTTGCTGCCATACCACTTAAGGAGCTTGAGGAGGAAGAGGAAAAGAGTGCAGTTCCTATTTTACCTCTTCACCGGCGTTTGCAGATCGAGTTCCACTACACCTCCTGCCCCTCCTCCTCCTCCTCTTCTTTGTCGACCTCTGCTACTTCTTCCACTTACTCTCTCTCATCAGGTGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

621

Amino Acids

69.93

Weight (kDa)

5.9

Isoelectric Point (pI)

56.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 34 - 149 7.3e-08 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 412
Acc16I TGCGCA 1 cut(s) 1137
AccB7I CCANNNNNTGG 1 cut(s) 170
AccI GTMKAC 2 cut(s) 1660, 1814
AciI CCGC 5 cut(s) 27, 341, 362, 1095, 1127
AclWI GGATC 1 cut(s) 1170
AcoI YGGCCR 1 cut(s) 17
AcsI RAATTY 3 cut(s) 251, 1181, 1562
AcuI CTGAAG 4 cut(s) 83, 580, 1330, 1499
AcvI CACGTG 1 cut(s) 46
AfaI GTAC 1 cut(s) 946
AfiI CCNNNNNNNGG 5 cut(s) 26, 170, 1126, 1215, 1282
AflII CTTAAG 1 cut(s) 1697
AgsI TTSAA 8 cut(s) 239, 385, 448, 741, 829, 1280, 1372, 1487
AhlI ACTAGT 1 cut(s) 875
AjnI CCWGG 5 cut(s) 139, 148, 185, 302, 1545
AjuI GAANNNNNNNTTGG 2 cut(s) 1253, 1285
AloI GAACNNNNNNTCC 2 cut(s) 144, 176
Alw26I GTCTC 4 cut(s) 420, 767, 1232, 1321
AlwI GGATC 1 cut(s) 1170
Ama87I CYCGRG 3 cut(s) 763, 787, 808
AoxI GGCC 3 cut(s) 17, 146, 1092
ApeKI GCWGC 5 cut(s) 67, 190, 1144, 1230, 1685
ApoI RAATTY 3 cut(s) 251, 1181, 1562
ArsI GACNNNNNNTTYG 4 cut(s) 734, 766, 1454, 1486
Asp700I GAANNNNTTC 1 cut(s) 181
AspA2I CCTAGG 1 cut(s) 842
AspLEI GCGC 1 cut(s) 1138
AspS9I GGNCC 3 cut(s) 146, 372, 640
AsuC2I CCSGG 3 cut(s) 644, 659, 1352
AsuHPI GGTGA 5 cut(s) 64, 130, 523, 1532, 1742
AsuII TTCGAA 1 cut(s) 580
AvaI CYCGRG 3 cut(s) 763, 787, 808
AvaII GGWCC 2 cut(s) 372, 640
AvrII CCTAGG 1 cut(s) 842
BanII GRGCYC 1 cut(s) 861
BbrPI CACGTG 1 cut(s) 46
BbsI GAAGAC 2 cut(s) 841, 878
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 5 cut(s) 54, 202, 1131, 1242, 1672
BccI CCATC 1 cut(s) 1025
BceAI ACGGC 1 cut(s) 4
BcgI CGANNNNNNTGC 2 cut(s) 1564, 1598
BciT130I CCWGG 5 cut(s) 141, 150, 187, 304, 1547
BclI TGATCA 1 cut(s) 1075
BcnI CCSGG 3 cut(s) 644, 659, 1352
BcoDI GTCTC 4 cut(s) 420, 767, 1232, 1321
BcuI ACTAGT 1 cut(s) 875
BfaI CTAG 4 cut(s) 389, 843, 876, 1861
BfmI CTRYAG 1 cut(s) 1228
BfrI CTTAAG 1 cut(s) 1697
BglI GCCNNNNNGGC 1 cut(s) 145
BglII AGATCT 1 cut(s) 179
BisI GCNGC 6 cut(s) 68, 191, 1095, 1145, 1231, 1686
BlnI CCTAGG 1 cut(s) 842
BlsI GCNGC 6 cut(s) 69, 192, 1096, 1146, 1232, 1687
Bme1390I CCNGG 8 cut(s) 141, 150, 187, 304, 644, 659, 1352, 1547
Bme18I GGWCC 2 cut(s) 372, 640
BmeT110I CYCGRG 3 cut(s) 763, 787, 808
BmgT120I GGNCC 3 cut(s) 146, 372, 640
BmiI GGNNCC 4 cut(s) 162, 373, 858, 1207
BmrFI CCNGG 8 cut(s) 141, 150, 187, 304, 644, 659, 1352, 1547
BmrI ACTGGG 1 cut(s) 704
BmsI GCATC 3 cut(s) 410, 1483, 1587
BmuI ACTGGG 1 cut(s) 704
BoxI GACNNNNGTC 1 cut(s) 1381
BpiI GAAGAC 2 cut(s) 841, 878
BpmI CTGGAG 2 cut(s) 162, 171
Bpu10I CCTNAGC 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 580
BpuEI CTTGAG 1 cut(s) 1727
BpuMI CCSGG 3 cut(s) 644, 659, 1352
BsaAI YACGTR 1 cut(s) 46
BsaI GGTCTC 1 cut(s) 1232
BsaJI CCNNGG 7 cut(s) 14, 302, 303, 425, 643, 842, 1436
BsaXI ACNNNNNCTCC 4 cut(s) 144, 174, 1766, 1796
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 170, 1126, 1215, 1282
Bse118I RCCGGY 1 cut(s) 1752
Bse1I ACTGG 2 cut(s) 699, 1145
Bse3DI GCAATG 1 cut(s) 1131
BseBI CCWGG 5 cut(s) 141, 150, 187, 304, 1547
BseDI CCNNGG 7 cut(s) 14, 302, 303, 425, 643, 842, 1436
BseGI GGATG 2 cut(s) 250, 1615
BseLI CCNNNNNNNGG 5 cut(s) 26, 170, 1126, 1215, 1282
BseMI GCAATG 1 cut(s) 1131
BseMII CTCAG 7 cut(s) 431, 498, 696, 791, 931, 1224, 1314
BseNI ACTGG 2 cut(s) 699, 1145
BseRI GAGGAG 5 cut(s) 1724, 1783, 1786, 1789, 1792
BseXI GCAGC 5 cut(s) 54, 202, 1131, 1242, 1672
BseYI CCCAGC 2 cut(s) 467, 1002
BsgI GTGCAG 2 cut(s) 1096, 1749
BshFI GGCC 3 cut(s) 19, 148, 1094
BsiHKCI CYCGRG 3 cut(s) 763, 787, 808
BsiSI CCGG 5 cut(s) 643, 658, 1098, 1352, 1753
BslFI GGGAC 6 cut(s) 285, 385, 472, 674, 896, 1389
BslI CCNNNNNNNGG 5 cut(s) 26, 170, 1126, 1215, 1282
BsmAI GTCTC 4 cut(s) 420, 767, 1232, 1321
BsmFI GGGAC 6 cut(s) 285, 385, 472, 674, 896, 1389
BsmI GAATGC 1 cut(s) 1147
BsnI GGCC 3 cut(s) 19, 148, 1094
Bso31I GGTCTC 1 cut(s) 1232
BsoBI CYCGRG 3 cut(s) 763, 787, 808
Bsp119I TTCGAA 1 cut(s) 580
Bsp1286I GDGCHC 1 cut(s) 861
Bsp143I GATC 6 cut(s) 179, 970, 1075, 1175, 1297, 1764
BspACI CCGC 5 cut(s) 27, 341, 362, 1095, 1127
BspANI GGCC 3 cut(s) 19, 148, 1094
BspCNI CTCAG 7 cut(s) 430, 499, 695, 790, 930, 1223, 1315
BspHI TCATGA 2 cut(s) 769, 973
BspLI GGNNCC 4 cut(s) 162, 373, 858, 1207
BspMAI CTGCAG 1 cut(s) 1232
BspPI GGATC 1 cut(s) 1170
BspT104I TTCGAA 1 cut(s) 580
BspTI CTTAAG 1 cut(s) 1697
BspTNI GGTCTC 1 cut(s) 1232
BsrDI GCAATG 1 cut(s) 1131
BsrFI RCCGGY 1 cut(s) 1752
BsrI ACTGG 2 cut(s) 699, 1145
BssAI RCCGGY 1 cut(s) 1752
BssECI CCNNGG 7 cut(s) 14, 302, 303, 425, 643, 842, 1436
BssMI GATC 6 cut(s) 179, 970, 1075, 1175, 1297, 1764
BssT1I CCWWGG 1 cut(s) 842
Bst2UI CCWGG 5 cut(s) 141, 150, 187, 304, 1547
Bst4CI ACNGT 4 cut(s) 376, 716, 1285, 1665
Bst6I CTCTTC 7 cut(s) 577, 623, 791, 809, 1710, 1752, 1810
BstAFI CTTAAG 1 cut(s) 1697
BstAPI GCANNNNNTGC 1 cut(s) 1144
BstBAI YACGTR 1 cut(s) 46
BstBI TTCGAA 1 cut(s) 580
BstC8I GCNNGC 3 cut(s) 59, 1038, 1111
BstDEI CTNAG 9 cut(s) 135, 417, 507, 682, 777, 917, 1210, 1301, 1323
BstDSI CCRYGG 1 cut(s) 14
BstF5I GGATG 2 cut(s) 250, 1615
BstHHI GCGC 1 cut(s) 1138
BstKTI GATC 6 cut(s) 182, 973, 1078, 1178, 1300, 1767
BstMAI GTCTC 4 cut(s) 420, 767, 1232, 1321
BstMBI GATC 6 cut(s) 179, 970, 1075, 1175, 1297, 1764
BstNI CCWGG 5 cut(s) 141, 150, 187, 304, 1547
BstPAI GACNNNNGTC 1 cut(s) 1381
BstSCI CCNGG 8 cut(s) 139, 148, 185, 302, 642, 657, 1350, 1545
BstSFI CTRYAG 1 cut(s) 1228
BstV1I GCAGC 5 cut(s) 54, 202, 1131, 1242, 1672
BstV2I GAAGAC 2 cut(s) 841, 878
BstX2I RGATCY 2 cut(s) 179, 1175
BstYI RGATCY 2 cut(s) 179, 1175
BsuRI GGCC 3 cut(s) 19, 148, 1094
BtgI CCRYGG 1 cut(s) 14
BtgZI GCGATG 1 cut(s) 633
BtsCI GGATG 2 cut(s) 250, 1615
BtsI GCAGTG 2 cut(s) 897, 1648
BtsIMutI CAGTG 5 cut(s) 692, 897, 925, 1138, 1648
Cac8I GCNNGC 3 cut(s) 59, 1038, 1111
CciI TCATGA 2 cut(s) 769, 973
CfoI GCGC 1 cut(s) 1138
Cfr10I RCCGGY 1 cut(s) 1752
Cfr13I GGNCC 3 cut(s) 146, 372, 640
CseI GACGC 1 cut(s) 1056
Csp6I GTAC 1 cut(s) 945
CviAII CATG 7 cut(s) 649, 770, 974, 980, 1381, 1582, 1586
CviQI GTAC 1 cut(s) 945
DdeI CTNAG 9 cut(s) 135, 417, 507, 682, 777, 917, 1210, 1301, 1323
DpnI GATC 6 cut(s) 181, 972, 1077, 1177, 1299, 1766
DpnII GATC 6 cut(s) 179, 970, 1075, 1175, 1297, 1764
DrdI GACNNNNNNGTC 1 cut(s) 412
DseDI GACNNNNNNGTC 1 cut(s) 412
EaeI YGGCCR 1 cut(s) 17
Eam1104I CTCTTC 7 cut(s) 577, 623, 791, 809, 1710, 1752, 1810
EarI CTCTTC 7 cut(s) 577, 623, 791, 809, 1710, 1752, 1810
EciI GGCGGA 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 842
Eco24I GRGCYC 1 cut(s) 861
Eco31I GGTCTC 1 cut(s) 1232
Eco32I GATATC 1 cut(s) 936
Eco47I GGWCC 2 cut(s) 372, 640
Eco57I CTGAAG 4 cut(s) 83, 580, 1330, 1499
Eco72I CACGTG 1 cut(s) 46
Eco88I CYCGRG 3 cut(s) 763, 787, 808
EcoO109I RGGNCCY 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 251
EcoRII CCWGG 5 cut(s) 139, 148, 185, 302, 1545
EcoRV GATATC 1 cut(s) 936
EcoT14I CCWWGG 1 cut(s) 842
EcoT22I ATGCAT 2 cut(s) 1498, 1587
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 842
FaeI CATG 7 cut(s) 652, 773, 977, 983, 1384, 1585, 1589
FalI AAGNNNNNCTT 6 cut(s) 166, 198, 465, 497, 1284, 1316
FaqI GGGAC 6 cut(s) 285, 385, 472, 674, 896, 1389
FatI CATG 7 cut(s) 648, 769, 973, 979, 1380, 1581, 1585
FauNDI CATATG 1 cut(s) 1397
FbaI TGATCA 1 cut(s) 1075
FblI GTMKAC 2 cut(s) 1660, 1814
Fnu4HI GCNGC 6 cut(s) 68, 191, 1095, 1145, 1231, 1686
FokI GGATG 2 cut(s) 257, 1622
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 6 cut(s) 68, 191, 1095, 1145, 1231, 1686
FspBI CTAG 4 cut(s) 389, 843, 876, 1861
FspI TGCGCA 1 cut(s) 1137
GlaI GCGC 1 cut(s) 1137
GluI GCNGC 6 cut(s) 68, 191, 1095, 1145, 1231, 1686
GsaI CCCAGC 2 cut(s) 471, 1006
GsuI CTGGAG 2 cut(s) 162, 171
HaeIII GGCC 3 cut(s) 19, 148, 1094
HapII CCGG 5 cut(s) 643, 658, 1098, 1352, 1753
HgaI GACGC 1 cut(s) 1056
HhaI GCGC 1 cut(s) 1138
Hin1II CATG 7 cut(s) 652, 773, 977, 983, 1384, 1585, 1589
Hin6I GCGC 1 cut(s) 1136
HinP1I GCGC 1 cut(s) 1136
HincII GTYRAC 3 cut(s) 712, 907, 1815
HindII GTYRAC 3 cut(s) 712, 907, 1815
HindIII AAGCTT 1 cut(s) 499
HpaI GTTAAC 1 cut(s) 712
HpaII CCGG 5 cut(s) 643, 658, 1098, 1352, 1753
HphI GGTGA 5 cut(s) 64, 130, 523, 1532, 1742
Hpy166II GTNNAC 4 cut(s) 712, 907, 1661, 1815
Hpy8I GTNNAC 4 cut(s) 712, 907, 1661, 1815
Hpy99I CGWCG 2 cut(s) 1394, 1571
HpyAV CCTTC 5 cut(s) 43, 82, 680, 1366, 1474
HpyCH4III ACNGT 4 cut(s) 376, 716, 1285, 1665
HpyCH4IV ACGT 1 cut(s) 45
HpyF3I CTNAG 9 cut(s) 135, 417, 507, 682, 777, 917, 1210, 1301, 1323
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 7 cut(s) 652, 773, 977, 983, 1384, 1585, 1589
HspAI GCGC 1 cut(s) 1136
Ksp22I TGATCA 1 cut(s) 1075
KspAI GTTAAC 1 cut(s) 712
Kzo9I GATC 6 cut(s) 179, 970, 1075, 1175, 1297, 1764
LmnI GCTCC 4 cut(s) 343, 600, 856, 1702
Lsp1109I GCAGC 5 cut(s) 54, 202, 1131, 1242, 1672
LweI GCATC 3 cut(s) 410, 1483, 1587
MaeI CTAG 4 cut(s) 389, 843, 876, 1861
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 4 cut(s) 324, 653, 690, 1541
MalI GATC 6 cut(s) 181, 972, 1077, 1177, 1299, 1766
MboI GATC 6 cut(s) 179, 970, 1075, 1175, 1297, 1764
MflI RGATCY 2 cut(s) 179, 1175
MhlI GDGCHC 1 cut(s) 861
MluCI AATT 9 cut(s) 79, 251, 268, 395, 897, 940, 997, 1181, 1562
MmeI TCCRAC 1 cut(s) 1436
Mph1103I ATGCAT 2 cut(s) 1498, 1587
MroXI GAANNNNTTC 1 cut(s) 181
MseI TTAA 4 cut(s) 78, 711, 1671, 1698
MslI CAYNNNNRTG 1 cut(s) 978
MspA1I CMGCKG 1 cut(s) 61
MspCI CTTAAG 1 cut(s) 1697
MspI CCGG 5 cut(s) 643, 658, 1098, 1352, 1753
MspR9I CCNGG 8 cut(s) 141, 150, 187, 304, 644, 659, 1352, 1547
Mva1269I GAATGC 1 cut(s) 1147
MvaI CCWGG 5 cut(s) 141, 150, 187, 304, 1547
NciI CCSGG 3 cut(s) 644, 659, 1352
NdeI CATATG 1 cut(s) 1397
NdeII GATC 6 cut(s) 179, 970, 1075, 1175, 1297, 1764
NlaIII CATG 7 cut(s) 652, 773, 977, 983, 1384, 1585, 1589
NlaIV GGNNCC 4 cut(s) 162, 373, 858, 1207
NmuCI GTSAC 2 cut(s) 653, 690
NsbI TGCGCA 1 cut(s) 1137
NsiI ATGCAT 2 cut(s) 1498, 1587
NspV TTCGAA 1 cut(s) 580
PaeR7I CTCGAG 1 cut(s) 808
PagI TCATGA 2 cut(s) 769, 973
PasI CCCWGGG 1 cut(s) 303
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PctI GAATGC 1 cut(s) 1147
PdmI GAANNNNTTC 1 cut(s) 181
PfeI GAWTC 5 cut(s) 421, 923, 1341, 1571, 1613
PflFI GACNNNGTC 2 cut(s) 556, 778
PflMI CCANNNNNTGG 1 cut(s) 170
PkrI GCNGC 6 cut(s) 69, 192, 1096, 1146, 1232, 1687
PmaCI CACGTG 1 cut(s) 46
PmlI CACGTG 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 46
PshAI GACNNNNGTC 1 cut(s) 1381
Psp6I CCWGG 5 cut(s) 139, 148, 185, 302, 1545
PspCI CACGTG 1 cut(s) 46
PspFI CCCAGC 2 cut(s) 467, 1002
PspGI CCWGG 5 cut(s) 139, 148, 185, 302, 1545
PspN4I GGNNCC 4 cut(s) 162, 373, 858, 1207
PspPI GGNCC 3 cut(s) 146, 372, 640
PstI CTGCAG 1 cut(s) 1232
PsuI RGATCY 2 cut(s) 179, 1175
PsyI GACNNNGTC 2 cut(s) 556, 778
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 1 cut(s) 946
RsaNI GTAC 1 cut(s) 945
RseI CAYNNNNRTG 1 cut(s) 978
SalI GTCGAC 1 cut(s) 1813
SaqAI TTAA 4 cut(s) 78, 711, 1671, 1698
SatI GCNGC 6 cut(s) 68, 191, 1095, 1145, 1231, 1686
Sau3AI GATC 6 cut(s) 179, 970, 1075, 1175, 1297, 1764
Sau96I GGNCC 3 cut(s) 146, 372, 640
ScrFI CCNGG 8 cut(s) 141, 150, 187, 304, 644, 659, 1352, 1547
SduI GDGCHC 1 cut(s) 861
SfaNI GCATC 3 cut(s) 410, 1483, 1587
SfcI CTRYAG 1 cut(s) 1228
Sfr274I CTCGAG 1 cut(s) 808
SfuI TTCGAA 1 cut(s) 580
SgrAI CRCCGGYG 1 cut(s) 1752
SinI GGWCC 2 cut(s) 372, 640
SlaI CTCGAG 1 cut(s) 808
SmiMI CAYNNNNRTG 1 cut(s) 978
SmlI CTYRAG 3 cut(s) 808, 1697, 1706
SmoI CTYRAG 3 cut(s) 808, 1697, 1706
SpeI ACTAGT 1 cut(s) 875
Sse9I AATT 9 cut(s) 79, 251, 268, 395, 897, 940, 997, 1181, 1562
SsiI CCGC 5 cut(s) 27, 341, 362, 1095, 1127
SspI AATATT 1 cut(s) 956
SspMI CTAG 4 cut(s) 389, 843, 876, 1861
StyD4I CCNGG 8 cut(s) 139, 148, 185, 302, 642, 657, 1350, 1545
StyI CCWWGG 1 cut(s) 842
TaaI ACNGT 4 cut(s) 376, 716, 1285, 1665
TaiI ACGT 1 cut(s) 48
TaqII GACCGA 1 cut(s) 530
TasI AATT 9 cut(s) 79, 251, 268, 395, 897, 940, 997, 1181, 1562
TatI WGTACW 1 cut(s) 944
TauI GCSGC 1 cut(s) 1097
TfiI GAWTC 5 cut(s) 421, 923, 1341, 1571, 1613
Tru1I TTAA 4 cut(s) 78, 711, 1671, 1698
Tru9I TTAA 4 cut(s) 78, 711, 1671, 1698
TscAI CASTG 5 cut(s) 699, 897, 925, 1145, 1648
TseFI GTSAC 2 cut(s) 653, 690
TseI GCWGC 5 cut(s) 67, 190, 1144, 1230, 1685
Tsp45I GTSAC 2 cut(s) 653, 690
TspDTI ATGAA 3 cut(s) 17, 264, 1657
TspGWI ACGGA 3 cut(s) 345, 892, 1096
TspRI CASTG 5 cut(s) 699, 897, 925, 1145, 1648
Tth111I GACNNNGTC 2 cut(s) 556, 778
Van91I CCANNNNNTGG 1 cut(s) 170
Vha464I CTTAAG 1 cut(s) 1697
VpaK11BI GGWCC 2 cut(s) 372, 640
XapI RAATTY 3 cut(s) 251, 1181, 1562
XhoI CTCGAG 1 cut(s) 808
XmaJI CCTAGG 1 cut(s) 842
XmiI GTMKAC 2 cut(s) 1660, 1814
XmnI GAANNNNTTC 1 cut(s) 181
XspI CTAG 4 cut(s) 389, 843, 876, 1861
Zsp2I ATGCAT 2 cut(s) 1498, 1587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.