AT2G27480

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011144
Physical Location & Seq
Forward (+)
11746683 .. 11748051
1369 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27480.1

Sequence Viewer

Length: 687 bp
ATGTCTTACTCAAACGCCTACGCACCGTCGGCACCGGAGCTACCAGAATCGTTCGTTCAGCAGCAGCACGATGGTGAAAGTAGATACACTTACGCGTATCCATCATATCAACCAACTCAGCAATTCAGCTCTTACTCCGGGATGTTTTCCCCGGAAACGCATCCGGAGATTGTGAGGAGCTTTGAGTCGGCGGATAGAAACCGGAGCGGATTTCTCGAAGAATCGGAGCTCCGGCAAGCGTTGTCGTTATCTGGGTACGATGGAATTAGTAACAGAACGATTAGGTTGCTGTTGTTTATTTACAAGATTCCTGTAGATTCTCTGCTTCGGCTTGGTCCTAAGGAGTATGTTGAGTTATGGAACTGCCTTGCGCAATGGCGTGCAATATTTAACAGGTACGATAGAGATAGGAGTGGAAAGATGAATTCTACCCAGCTCAGAGACGCTTTCTATAATCTCGGGTGCGTGCTTCCAACCTCGGTTCACCAGCTTATCGTATCTCAGTTTGATGATGGGACTGGCAAAACTGTTGACCTATGTTTCGACAGCTTCCTCGAGTGTGGGATGATTGTGAAGGGATTGACAGAGAAGTTCAGGGAGAACGACCCTGGTTACACAGGCTACGCAACGCTCTCTTACGATGTCTTCATGTTGATGGTCATTCCGTTCATCGCCACCTATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

228

Amino Acids

26.13

Weight (kDa)

4.94

Isoelectric Point (pI)

52.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27480 AT2G27480
fragaria_vesca FvH4_3g24940
malus_domestica MD00G1075300.v1.1 MD03G1144700.v1.1 MD11G1164500.v1.1
prunus_persica Prupe.6G130400_v2.0.a1 Prupe.6G130400_v2.0.a1
pyrus_communis pycom11g13680
rosa_chinensis RchiOBHm_Chr5g0044731
rosa_laevigata RLG00000034294
rosa_multiflora Rmu_sc0000228.1_g000011
rosa_roxburghii Rroxscaffold_1G00036300
rosa_rugosa Rorug05G0217200
rosa_samantha Rh5AG300500 Rh5BG307200 Rh5CG334200 Rh5DG317300
rosa_wichuraiana Rw5G027800 Rw5G027840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 31
AccBSI CCGCTC 1 cut(s) 207
AccII CGCG 1 cut(s) 95
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 2 cut(s) 191, 207
AcsI RAATTY 1 cut(s) 424
AfaI GTAC 2 cut(s) 257, 398
AflIII ACRYGT 1 cut(s) 93
AjnI CCWGG 1 cut(s) 607
AleI CACNNNNGTG 1 cut(s) 72
AluBI AGCT 7 cut(s) 40, 129, 180, 229, 436, 490, 549
AluI AGCT 7 cut(s) 40, 129, 180, 229, 436, 490, 549
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 435
Ama87I CYCGRG 2 cut(s) 458, 554
Aor13HI TCCGGA 1 cut(s) 163
ApeKI GCWGC 2 cut(s) 61, 64
ApoI RAATTY 1 cut(s) 424
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
AspLEI GCGC 1 cut(s) 373
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 2 cut(s) 139, 152
AsuHPI GGTGA 2 cut(s) 86, 476
AvaI CYCGRG 2 cut(s) 458, 554
AvaII GGWCC 1 cut(s) 335
AxyI CCTNAGG 1 cut(s) 339
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 637
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 73, 76
BccI CCATC 5 cut(s) 65, 109, 254, 506, 649
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 1 cut(s) 609
BciVI GTATCC 1 cut(s) 108
BcnI CCSGG 2 cut(s) 139, 152
BcoDI GTCTC 1 cut(s) 435
BfmI CTRYAG 1 cut(s) 312
BfuI GTATCC 1 cut(s) 108
BisI GCNGC 2 cut(s) 62, 65
BlsI GCNGC 2 cut(s) 63, 66
Bme1390I CCNGG 3 cut(s) 139, 152, 609
Bme18I GGWCC 1 cut(s) 335
BmeT110I CYCGRG 2 cut(s) 458, 554
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 3 cut(s) 139, 152, 609
BmsI GCATC 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 637
BpuMI CCSGG 2 cut(s) 139, 152
BsaJI CCNNGG 3 cut(s) 150, 477, 607
BsaWI WCCGGW 3 cut(s) 34, 163, 201
Bse1I ACTGG 1 cut(s) 523
Bse21I CCTNAGG 1 cut(s) 339
Bse3DI GCAATG 1 cut(s) 380
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 3 cut(s) 150, 477, 607
BseGI GGATG 3 cut(s) 147, 160, 570
BseMI GCAATG 1 cut(s) 380
BseMII CTCAG 3 cut(s) 131, 451, 515
BseNI ACTGG 1 cut(s) 523
BseRI GAGGAG 1 cut(s) 190
BseXI GCAGC 2 cut(s) 73, 76
BseYI CCCAGC 1 cut(s) 432
Bsh1236I CGCG 1 cut(s) 95
BshNI GGYRCC 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 2 cut(s) 458, 554
BsiSI CCGG 6 cut(s) 35, 138, 152, 164, 202, 232
BslFI GGGAC 1 cut(s) 529
BsmAI GTCTC 1 cut(s) 435
BsmBI CGTCTC 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 529
BsoBI CYCGRG 2 cut(s) 458, 554
Bsp1286I GDGCHC 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 163
BspACI CCGC 2 cut(s) 191, 207
BspCNI CTCAG 3 cut(s) 130, 450, 514
BspEI TCCGGA 1 cut(s) 163
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 1 cut(s) 33
BspT107I GGYRCC 1 cut(s) 31
BsrBI CCGCTC 1 cut(s) 207
BsrDI GCAATG 1 cut(s) 380
BsrI ACTGG 1 cut(s) 523
BssECI CCNNGG 3 cut(s) 150, 477, 607
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 2 cut(s) 27, 529
BstC8I GCNNGC 3 cut(s) 237, 381, 467
BstDEI CTNAG 4 cut(s) 117, 339, 437, 501
BstF5I GGATG 3 cut(s) 147, 160, 570
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 373
BstMAI GTCTC 1 cut(s) 435
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 1 cut(s) 609
BstSCI CCNGG 3 cut(s) 137, 150, 607
BstSFI CTRYAG 1 cut(s) 312
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 73, 76
BstV2I GAAGAC 1 cut(s) 637
Bsu36I CCTNAGG 1 cut(s) 339
BsuI GTATCC 1 cut(s) 108
BtgZI GCGATG 1 cut(s) 655
BtsCI GGATG 3 cut(s) 147, 160, 570
Cac8I GCNNGC 3 cut(s) 237, 381, 467
CfoI GCGC 1 cut(s) 373
Cfr13I GGNCC 1 cut(s) 335
CseI GACGC 1 cut(s) 452
Csp6I GTAC 2 cut(s) 256, 397
CviAII CATG 1 cut(s) 649
CviJI RGCY 9 cut(s) 40, 129, 180, 229, 331, 436, 490, 549, 621
CviKI_1 RGCY 9 cut(s) 40, 129, 180, 229, 331, 436, 490, 549, 621
CviQI GTAC 2 cut(s) 256, 397
DdeI CTNAG 4 cut(s) 117, 339, 437, 501
EciI GGCGGA 1 cut(s) 206
Ecl136II GAGCTC 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 335
Eco53kI GAGCTC 1 cut(s) 229
Eco81I CCTNAGG 1 cut(s) 339
Eco88I CYCGRG 2 cut(s) 458, 554
EcoICRI GAGCTC 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 607
EcoT38I GRGCYC 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 1 cut(s) 652
FaiI YATR 7 cut(s) 106, 348, 358, 453, 538, 650, 681
FaqI GGGAC 1 cut(s) 529
FatI CATG 1 cut(s) 648
Fnu4HI GCNGC 2 cut(s) 62, 65
FokI GGATG 3 cut(s) 147, 154, 577
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 2 cut(s) 62, 65
FspI TGCGCA 1 cut(s) 372
GlaI GCGC 1 cut(s) 372
GluI GCNGC 2 cut(s) 62, 65
GsaI CCCAGC 1 cut(s) 436
HapII CCGG 6 cut(s) 35, 138, 152, 164, 202, 232
HgaI GACGC 1 cut(s) 452
HhaI GCGC 1 cut(s) 373
Hin1II CATG 1 cut(s) 652
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HinfI GANTC 5 cut(s) 47, 185, 221, 307, 317
HpaII CCGG 6 cut(s) 35, 138, 152, 164, 202, 232
HphI GGTGA 2 cut(s) 86, 476
Hpy166II GTNNAC 2 cut(s) 484, 532
Hpy188I TCNGA 2 cut(s) 226, 440
Hpy188III TCNNGA 2 cut(s) 164, 215
Hpy8I GTNNAC 2 cut(s) 484, 532
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 1 cut(s) 568
HpyCH4III ACNGT 2 cut(s) 27, 529
HpyCH4V TGCA 1 cut(s) 383
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 4 cut(s) 117, 339, 437, 501
Hsp92II CATG 1 cut(s) 652
HspAI GCGC 1 cut(s) 371
Kpn2I TCCGGA 1 cut(s) 163
LmnI GCTCC 5 cut(s) 37, 177, 204, 226, 234
Lsp1109I GCAGC 2 cut(s) 73, 76
LweI GCATC 1 cut(s) 169
MaeIII GTNAC 2 cut(s) 269, 611
MbiI CCGCTC 1 cut(s) 207
MboII GAAGA 2 cut(s) 230, 637
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 3 cut(s) 122, 264, 424
MluI ACGCGT 1 cut(s) 93
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 1 cut(s) 497
MnlI CCTC 3 cut(s) 168, 487, 563
MroI TCCGGA 1 cut(s) 163
MseI TTAA 2 cut(s) 390, 685
MslI CAYNNNNRTG 2 cut(s) 72, 653
MspI CCGG 6 cut(s) 35, 138, 152, 164, 202, 232
MspR9I CCNGG 3 cut(s) 139, 152, 609
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 29
NciI CCSGG 2 cut(s) 139, 152
NlaIII CATG 1 cut(s) 652
NlaIV GGNNCC 1 cut(s) 33
NsbI TGCGCA 1 cut(s) 372
OliI CACNNNNGTG 1 cut(s) 72
PaeR7I CTCGAG 1 cut(s) 554
PfeI GAWTC 4 cut(s) 47, 221, 307, 317
PfoI TCCNGGA 1 cut(s) 137
PkrI GCNGC 2 cut(s) 63, 66
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 607
PspFI CCCAGC 1 cut(s) 432
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 335
PspXI VCTCGAGB 1 cut(s) 554
RsaI GTAC 2 cut(s) 257, 398
RsaNI GTAC 2 cut(s) 256, 397
RseI CAYNNNNRTG 2 cut(s) 72, 653
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 2 cut(s) 390, 685
SatI GCNGC 2 cut(s) 62, 65
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 3 cut(s) 139, 152, 609
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 1 cut(s) 169
SfcI CTRYAG 1 cut(s) 312
Sfr274I CTCGAG 1 cut(s) 554
SinI GGWCC 1 cut(s) 335
SlaI CTCGAG 1 cut(s) 554
SmiMI CAYNNNNRTG 2 cut(s) 72, 653
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 3 cut(s) 122, 264, 424
SsiI CCGC 2 cut(s) 191, 207
SspI AATATT 1 cut(s) 387
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 3 cut(s) 137, 150, 607
TaaI ACNGT 2 cut(s) 27, 529
TaqI TCGA 3 cut(s) 216, 543, 555
TasI AATT 3 cut(s) 122, 264, 424
TfiI GAWTC 4 cut(s) 47, 221, 307, 317
Tru1I TTAA 2 cut(s) 390, 685
Tru9I TTAA 2 cut(s) 390, 685
TseI GCWGC 2 cut(s) 61, 64
TspDTI ATGAA 3 cut(s) 437, 637, 658
TspGWI ACGGA 1 cut(s) 654
VpaK11BI GGWCC 1 cut(s) 335
XapI RAATTY 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.